KR20200097005A - Composition for increase of muscle function comprising extract of juniperus rigida - Google Patents

Abstract

A composition for improving a muscle function according to the present invention includes an extract of Juniperus rigida. According to the composition for improving a muscle function derived from the present invention, the muscle function is improved and muscle damage is reduced.

Description

Composition for improving muscle function containing juniper extract {COMPOSITION FOR INCREASE OF MUSCLE FUNCTION COMPRISING EXTRACT OF JUNIPERUS RIGIDA}

The present invention relates to a composition for improving muscle function comprising an extract of Juniperus rigida, and having an effect of improving muscle function and improving muscle damage, and at the same time, relates to a composition for improving muscle function without side effects even when the dosage and duration of administration are increased. will be.

Muscle (Muscle) is a tissue produced by the development of stem cells of the mesoderm and is composed of a bundle of myofibers made up of a combination of muscle cells (myoblasts). Muscles account for 40-50% of their body weight, so they support and protect bones and internal organs, while at the same time allowing the motility of other tissues other than muscles such as the heartbeat. In addition to the protection of organs, muscles are closely related to the onset of metabolic diseases such as diabetes, obesity, and cardiovascular disease because muscles have a great influence on nutrient metabolism as well as physical activities including exercise.

Human muscles decrease by more than 1% every year from the age of 40, and by the age of 80, the level of the maximum muscle mass decreases by 50%, and the loss of muscles in old age is recognized as the most important factor in reducing the overall physical function. Therefore, the need to improve muscle function is increasing, and the development of therapeutic agents for diseases related to muscle function decline is also becoming important.

The most important factor in determining muscle function is muscle mass, which is maintained by a balance between protein synthesis and degradation. Factors involved in muscle protein synthesis induce protein synthesis by phosphorylating downstream proteins from stimulation of the PI3K (phosphatidylinositol-3 kinase/Akt pathway pathway) in muscle cells. Mammalian target (mTOR) by PI3K/Akt signaling. of rapamycin) is recognized as a central growth signaling factor that integrates various growth signals within the cell.The activation of mTOR is the two downstream targets, 4E-BP1 (4E-binding protein) and p70S6K (phosphorylated 70-). Activation of kDa ribosomal S6 kinase) induces muscle protein synthesis, contributing to the increase of muscle mass, which is indicated by an increase in muscle size (hypertrophy) or an increase in the number of muscle fibers (hyperplasia) according to the increase in protein.

Conversely, when FoxO (forhead box), a transcription factor, moves from the cytoplasm to the nucleus, it increases the expression of E3 ubiquitin ligase factors, atrogin-1 and MuRF-1, which are involved in protein degradation. In this case, protein breakdown in the muscle is promoted and muscle mass is reduced. In other words, protein degradation occurs more than synthesis, which causes muscle weakness and degeneration due to a decrease in muscle mass.

Therefore, promoting mTOR activity and suppressing the expression of atrogin-1 and MuRF-1 increase the amount of muscle protein, thereby increasing muscle mass, so mTOR, atrogin-1, and MuRF- The expression of 1 is an important factor in muscle mass regulation.

Accordingly, there is a need to develop a substance capable of increasing the expression of proteins related to muscle protein synthesis and muscle mass increase in muscle cells, and preventing and improving muscle weakness and degeneration through muscle mass control.

(Patent Document 1) KR 10-2017-0132705 A

It is an object of the present invention to provide a composition for improving muscle function comprising an extract of Juniperus rigida.

Another object of the present invention is to provide a composition for improving muscle function derived from natural products capable of increasing the expression of proteins related to muscle protein synthesis and muscle mass increase.

Another object of the present invention is to provide a composition for improving muscle function that does not have side effects and can be used periodically or for a long time.

In order to achieve the above object, the composition for improving muscle function according to an embodiment of the present invention includes an extract of Juniperus rigida.

The improvement of muscle function is to treat or prevent a disease selected from the group consisting of muscular atrophy, muscular dystrophy, sacopenia, myasthenia, and muscle degeneration.

The composition is any one selected from the group consisting of Pittosporum tobira Ait. extract, Cinnamomum japonicum Sieb. extract, Caesalpinia decapetala extract, Smilax chine L. extract, and mixtures thereof. It includes more.

The extract includes extraction using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols and mixtures thereof.

Functional food according to another embodiment of the present invention may include a composition for improving muscle function.

The external preparation for skin according to another embodiment of the present invention may include a composition for improving muscle function.

A pharmaceutical product according to another embodiment of the present invention may include a composition for improving muscle function.

Hereinafter, the present invention will be described in more detail.

The term'extract' as used herein has the meaning commonly used as a crude extract in the sugar leaf system as described above, but broadly includes a fraction obtained by additionally fractionating the extract. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (sized for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

In the present specification,'muscle' refers to tendons, muscles, and tendons generically, and'muscle function' refers to the ability to exert power by contraction of muscles, and the muscles can exert their contractile force as much as possible to overcome resistance. It includes muscle strength, the ability to be capable of, muscle endurance, the ability to express how long or how many times a muscle can contract and repeat the theory at a given weight, and quickness, the ability to exert strong power in a short time.

The composition for preventing and treating arthritis according to an embodiment of the present invention includes an extract of Juniperus rigida.

Juniperus rigida extract is also called juniper, needle juniper, and is an evergreen tall tall tree belonging to the cypress family, and is distributed throughout Korea, China, and Siberia. In Korea, it grows in a limestone area, and the circumference extends to the side. It is a small arboretum (J. conferta) with roots in the middle of it, and a small arboreous tree with a height of 15m. (J. communis), the leaf length is 4-8mm shorter than the basic species, and the fruit is 6-7mm longer than the leaves, and the juniper tree (J. communis var. montana) and the seaside juniper (Juniperus rigida Sieb.et) Zuccvar. Koreana T. Lee). It is used as a Bangpom Forest or Pungchi Forest near the coast or reclaimed land because it is resistant to cold and well withstands sea breezes. The juniper has a reddish brown bark, blooms in April, and has a dark brown ball shape and a little sweet taste. Not only can it grow well in a dry place, but it also has the characteristics that seeds can be spread by a bird frame.

In addition, the oil collected from the seeds of the juniper is known to have pharmacological effects such as diuretics, so it is used for edema, urinary tract and genital diseases, and is also used as an (pharmaceutical) insect repellent. In the West, juniper berry, the fruit of the juniper, was sealed with strong liquor to make it 3-4 times the amount of fruit, and stored for about 6 months, and then the ingredients were removed and stored for drinking or used as aroma.

As such, the existing studies using juniper are mostly for oriental medicine treatment or used as alcohol, and the effect as a composition for improving muscle function is insufficient.

The improvement of muscle function is to treat or prevent a disease selected from the group consisting of muscular atrophy, muscular dystrophy, sacopenia, myasthenia, and muscle degeneration.

Muscular atrophy is caused by a gradual decrease in muscle mass, and refers to muscle weakness and degeneration. Muscle atrophy is promoted by inactivity, oxidative stress, and chronic inflammation, which impairs muscle function and motor skills.

Muscular dystrophy is a progressive, incurable and hereditary disease in which skeletal muscles are gradually degenerated and atrophy and worsen. In muscular dystrophy, degeneration of the skeletal muscles proceeds regardless of the central nervous system and the peripheral nervous system, resulting in weakening, constriction, and deformation of the muscles, and large-changing muscular atrophy that comes from pseudo-contrast or progressive in certain muscles. Muscular dystrophy is sometimes used without distinction from muscular atrophy, but there are some differences in clinical symptoms. Muscular dystrophy occurs mainly in childhood, and muscular dystrophy occurs in adolescence. Also, muscular dystrophy occurs in the proximal muscles and muscular dystrophy occurs in the distal muscles. Muscular stiffness does not exist in muscular dystrophy, but in muscular atrophy, and muscular dystrophy has a certain heritability, but muscular atrophy has a rare genetic tendency.

Sacopenia is a disease caused by a decrease in muscle mass and mainly occurs due to a decrease in skeletal muscle mass due to aging. It is known that sarcopenia directly causes a decrease in muscle strength, and as a result, decreases and impairs various bodily functions, and increases the risk of death.

Myasthenia is a neurological disorder of the muscle, which is a disease in which the muscle is weakened and occurs when the nerve is stimulated. When nerve stimulation occurs, a chemical called acetylcholine is released from the nerve endings. When this chemical binds to the acetylcholine receptor in the fascia, muscle contraction occurs. However, if the shape of the acetylcholine receptor in the fascia is changed or other substances are blocking the acetylcholine receptor, if nerve stimulation is not transmitted properly, muscle contraction does not occur, and the muscle is weakened. Myasthenia gravis initially causes mild symptoms such as blepharoplasty (ptosis) and poor pupils, but when it gets worse, the symptoms spread throughout the body and the arms and legs do not have enough strength. Because of this, it is difficult to lift objects and fall easily, and dangerous conditions such as difficulty breathing and respiratory paralysis may appear.

The composition is any one selected from the group consisting of Pittosporum tobira Ait. extract, Cinnamomum japonicum Sieb. extract, Caesalpinia decapetala extract, Smilax chine L. extract, and mixtures thereof. It includes more.

Pittosporum tobira Ait. is an evergreen shrub of the Pittosporaceae family. It grows on the shores of Sanyana in the Adohae region in southern Korea. It grows to 2~3m in height, and the branches have no hairs, and the leaves of 4~10cm in length and 2~3cm in butterfly have a shiny dark green color and grow in a long obovate shape. The flowers that bloom in May-June with white chlorosis are bisexual and have a deep scent. Fruits are round or oval and mature around October. It has a subtle scent from its root bark, and its leaves and flowers are beautiful, so it is widely used as an ornamental plant in the southern regions. The leaves, stems, and bark of the pig tree are called chili-hyang and are used for high blood pressure, arteriosclerosis, and joint pain.

Cinnamomum japonicum Sieb. is an evergreen tree of the Lauraceae family, and mainly grows in the archipelago in southern Korea. It grows high up to 15m in height, and the bark of the mature stem is black, the young branches are green and there is no hair. Leaves are alternate, 6~15cm long, 2~5cm wide, narrow ovate or long oval, and the back side is pale white. It blooms around June as a pale yellow embrittlement, which is a benign flower. The oval fruit is about 12mm in diameter, and matures in purple black around December.

Caesalpinia decapetala (Caesalpinia decapetala) is a deciduous shrub belonging to the dicotyledonous rosewood legume family. It grows in the sun at the foot of the mountain, and is widely distributed in Korea, Japan, southern China, and India. The length is about 4m, and there are thorns bent downwards on the whole, the leaves are alternated and there are regenerated buds on the axils as pinnate compound leaves twice. Small leaves are 5-10 pairs each, elliptical, and about 1-2cm long. The fruit is a narrow fruit, flat oval, about 9cm long, about 2.7cm wide, not open. Silgori is mainly a fruit and is used in the private sector as a medicinal material such as an antipyretic or a branch medicine.

Smilax chine L. is distributed in mountainous areas nationwide, and is widely distributed in Korea, China, and Japan. According to the Korean Pharmacopoeia and Herbal Medicine (Pharmaceutical) Standards Book, it is also known as Tobokryeong or Ugye. It is a stem-root plant belonging to the lily family. It was used as a natural food preservative to preserve. In addition, the roots of blueberry vines are used to relieve symptoms such as fever, detoxification, and diuresis, improve physical strength, and as a treatment for inflammation such as dermatitis, nephritis, and cystitis.

In addition to the juniper extract, the composition for improving muscle function further comprises any one selected from the group consisting of a pig tree extract, a saengdal tree extract, a citrus tree extract, a blueberry extract, and a mixture thereof, and when used as a complex extract, the When used as a composition for improving muscle function, it can exhibit a more excellent effect.

Preferably, the composition of the present invention comprises 40 to 60 parts by weight of a pig tree extract, 40 to 60 parts by weight of a birch extract, 40 to 60 parts by weight of a citrus tree extract, and 40 to 60 parts by weight of a blueberry extract can do. When used as a complex extract within the above range, the effect of improving muscle function is further increased due to the complex action between each component.

In addition, when used as a complex extract within the above range, it exhibits an excellent effect on improving muscle function, and at the same time, makes it possible to provide a functional stone food composition having excellent preference.

The extract includes extraction using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols and mixtures thereof.

Specifically, in order to prepare a juniper extract, the step of pulverizing a natural product; Leaching the pulverized product using an organic solvent; Drying the sample after leaching; Re-leaching the dried sample using an organic solvent; Drying the sample after leaching; Leaching with water; And including the step of leaching, it is possible to obtain a natural extract.

The natural extract extracted using the organic solvent may further include performing fractionation using an organic solvent.

The method of preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and the extract may be extracted by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 mL of water and an alcohol having 1 to 6 carbon atoms, using 10 to 30 g of a pulverized product of a natural product, a reflux time of 1 to 3 hours, and an alcohol or water having 1 to 6 carbon atoms of 50 to 100%. More specifically, based on 100Ml of alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of a pulverized product of a natural product, a reflux time of 1 to 2 hours, and 70 to 90% of an alcohol or water having 1 to 4 carbon atoms.

The leaching method is performed at 15 to 30° C. for 24 to 72 hours, and water or 50 to 100% alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, it proceeds for 20 to 25° C. for 30 to 54 hours, and the extraction solvent is made of water or an alcohol having 1 to 6 carbon atoms of 70 to 80%.

The ultrasonic extraction method proceeds for a reaction at 30 to 50° C. for 0.5 to 2.5 hours, and the extraction solvent is water or an alcohol having 1 to 6 carbon atoms of 50 to 100%. Specifically, extraction is performed at 40 to 50° C. for 1 to 2.5 hours, and water or an alcohol having 1 to 6 carbon atoms of 70 to 80% is used as an extraction solvent.

The extraction solvent may be 2 to 50 times the weight of the sample, and more specifically 2 to 20 times. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and more specifically, for 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a new fractionation solvent. The fractionation solvent used for fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

Functional food according to another embodiment of the present invention may include a composition for improving muscle function.

"Functional food" as defined herein refers to a food manufactured and processed using raw materials or ingredients that have functions useful for the human body according to the Functional Food Act No.6727, and "functional" refers to the structure of the human body and It refers to ingestion for the purpose of obtaining useful effects for health purposes such as controlling nutrients for function or physiological action.

The external preparation for skin according to another embodiment of the present invention may include a composition for improving muscle function.

A pharmaceutical product according to another embodiment of the present invention may include a composition for improving muscle function.

The preferred dosage of the extract of the present invention varies depending on the condition and weight of the patient, the degree of disease, the form of the drug, the route and duration of administration, but may be appropriately selected by those skilled in the art. However, for a desirable effect, the extract is preferably administered at 0.01 mg/kg to 10 g/kg per day, preferably 1 mg/kg to 1 g/kg. Administration may be administered once a day, or may be divided several times. Therefore, the dosage does not limit the scope of the present invention in any way.

According to the composition for improving muscle function of the present invention, it may exhibit an effect of improving muscle function.

In the case of the composition for improving muscle function, an extract derived from a natural product is used to provide a composition that is effective for improving muscle function and improving muscle damage, but there is no problem of side effects, so it can be used periodically or for a long time.

1 shows the results of measuring the mRNA expression of atrogin-1 and MuRF-1 in C2C12 muscle cells according to treatment with nogan extract.
Figure 2 shows the muscle strength of the experimental animal according to the noganju extract treatment.
3 shows an increase in the weight of the right calf muscle of the experimental animal according to the treatment of juniper extract.
Figure 4 shows the results of measuring the mRNA expression of atrogin-1 and MuRF-1 according to the complex extract treatment in C2C12 muscle cells.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

[Production Example: Preparation of extract]

One. Preparation of juniper extract

After crushing dried juniper leaves and branches with a mixer, 100 g of crushed juniper powder was immersed in 1 L of 100%, 70%, 50%, or 30% ethanol and left at room temperature for 24 hours to obtain an extract. The process was repeated three times. The obtained extract was filtered under reduced pressure with Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to obtain a noganju extract (JE) from which the solvent component was removed.

2. Preparation of other natural extracts

Using the same method as the method for preparing the juniper extract (JE), pig tree extract (PE), saengdal tree extract (CE), citrus tree extract (DE), and blueberry vine extract (SE) were prepared.

3. Preparation of complex extract

The juniper extract (JE), pig tree extract (PE), rosewood extract (CE), citrus tree extract (DE), and blueberry extract (SE) were mixed within the weight range as shown in Table 1 below to prepare a composition for improving muscle function. I did.

MX1 MX2 MX3 MX4 MX5 JE 100 100 100 100 100 PE - 30 40 60 80 CE - 30 40 60 80 DE - 30 40 60 80 SE - 30 40 60 80

(Unit weight part)

[Experimental Example 1: Effect of improving muscle function of Noganzo extract]

One. Inhibitory effect of muscle protein degradation in muscle cells

Myoblasts derived from C2C12 (CRL1772 ATCC, USA) mice used in the experiment were purchased from the American type Cell Collection (ATCC, USA), and were cultured in DMEM (Dulbecco's Modified Eagle Medium) medium containing 10% FBS. At the time of differentiation, the cells were differentiated using a DMEM medium containing 2% horse serum, and cultured for an additional 6 days while changing the medium every 2 days.

Differentiated C2C12 muscle cells were treated with juniper extract (JE) and cultured for 12 hours to obtain cells. RNA was extracted from the obtained cells with a NucleoSpin RNA II kit (Macherey-Nagel, Duren, Germany). The extracted total RNA was cDNA synthesized using REVERTRA Ace qPCR RT Master[0110] Mix (TOYOBO, Osaka, Japan).

Of the synthesized cDNA, 3 μL of cDNA was mixed with specific primers (atrogin-1_F, atrogin-1_R, MuRF1_F, MuRF1_R, β-Actin_F, β-Actin_R) and PCR premix (ELPIS-Biotech), at 95°C for 30 seconds, 60 PCR was performed by repeating 1 minute at °C and 1 minute at 72°C 30 times. After PCR amplification, cDNA was separated by electrophoresis with 1.5% agarose gel, G; The cDNA band was confirmed using a BOX EF imaging system (Syngene).

As a result, it was confirmed that the mRNA expression levels of the muscle destruction proteins atrogin-1 and MuRF1 were decreased in C2C12 muscle cells as the noganju extract (JE) was treated as shown in FIG. 1. This means that noganju extract (JE) has an excellent ability to inhibit the degradation of muscle proteins in muscle cells, and it was confirmed that it is effective in improving muscle function.

2. Effect of juniper extract on improving muscle strength

As experimental animals, 7-week-old male mice (C57BL/6J; Daehan Biolink) were purchased and the experiment was conducted. A total of 28 mice were randomly assigned to 7 mice per group, and were divided into a normal group, an immobilization treatment group, a group administered with 10 μM of juniper extract (JE), and a group administered with 20 µM of juniper extract (JE).

After acclimation for 1 week, anesthesia was induced by intraperitoneal injection of 325 mg/kg tribromoethanol (Sigma-aldrich). After anesthesia, the right hind limb moves with a stapler shim using a skin stapler (Unidus, Chungcheongbuk-do, Korea) on the right hind limb of the mice belonging to the immobilization treatment group and the juniper extract (JE) 10 μM and 20 μM administration group. It was fixed so that it was not, and this state was maintained for a week. One week later, the stapler core fixed on the calf muscle and the sole of the foot was removed, and the juniper extract (JE) was administered orally daily at concentrations of 10 μM and 20 μM for a week. The normal group and the immobilized group were orally administered with saline.

After that, as a result of measuring the muscle strength of the rat using a muscle strength meter (Columbus Instrument, Columbus, OH, USA), as shown in Fig. 2, it was confirmed that the muscle strength was significantly reduced in the immobilized treatment group compared to the normal group. I did. In addition, it was confirmed that muscle strength was recovered in a concentration-dependent manner as the noganju extract (JE) was treated. This means that the noganju extract (JE) of the present invention has an excellent ability to increase muscle strength, and it was confirmed that it is effective in improving muscle function.

3. Effect of Juniper Extract on Damaged Muscle Regeneration

In the above experiment, after the muscle strength measurement was completed, 325 mg/kg tribromoethanol (Sigma-aldrich) was injected into the abdominal cavity of the experimental animal and sacrificed through cardiac blood collection after anesthesia.

After confirming that the heartbeat stopped, the calf muscle of the right hind limb physically damaged by a stapler was removed and the weight was measured.

As a result, as shown in FIG. 3, it was confirmed that the weight of the calf muscle was significantly reduced in the immobilization-treated group compared to the normal group, and the muscle weight increased in a concentration-dependent manner as the juniper extract (JE) was treated. Was confirmed.

This means that the juniper extract (JE) of the present invention has an excellent effect of promoting damaged muscle regeneration, and it was confirmed that it is effective in improving muscle function.

[Experimental Example 2: Effect of improving muscle function of complex extract]

In order to confirm the synergistic effect due to the interaction according to the use of the complex extract, the experiment was conducted in the same manner as the noganju extract (JE), which confirmed the muscle protein degradation inhibitory effect.

The results are shown in FIG. 4, and for comparison, the muscle protein inhibitory effect of 20 μM of the juniper extract (JE) is fixed to an index of 5 as a reference, and the result of using the complex extract is compared with an index of 1 to 10. Represented by The higher the number, the better the inhibitory activity, the better the muscle protein inhibitory activity.

The results are shown in the following [Table 2].

JE MX2 MX3 MX4 MX5 atrogin-1 inhibitory activity 5 4 7 7 6 MuRF1 inhibitory activity 5 3 4 6 4

(Unit: index)

Referring to [Table 2] above, it can be seen that a synergistic effect appears according to use in the complex extract. In particular, in the case of MX 3 to MX 4, it can be seen that the inhibitory effect of muscle proteins is greatly increased.

Therefore, in the case of the complex extract in the range of MX 3 to MX 4, it can be seen that synergistic effect by mixing each extract results in a synergistic effect more than a simple combination.

Accordingly, it can be seen that when the composite extract is used, it can exhibit excellent inhibitory activity against the production of muscle protein in a smaller amount. Therefore, it is possible to produce a product including a composition for improving muscle function, which can be taken for a long time with little side effects, since it can produce an effect on improving muscle function at a relatively low concentration through this.

[Experimental Example 3: palatability test]

One. Sensory evaluation test

A tea beverage was prepared by diluting MX 2 to MX 5 of JE and the complex extract. The tea drink was tasted by a tasting person, and taste and aroma were expressed in an index of 1 to 10, and the average value (rounded to 0.5) was shown in Table 3 below. The higher the number, the higher the preference.

JE MX2 MX3 MX4 MX5 flavor 5.5 4.5 6 6 5 incense 4.5 2.5 4 5 4 Overall preference (average) 5.5 4 5.5 6 5

(Unit: index)

Referring to Table 3, in the case of JE, the taste of the juniper extract was evaluated well, but its characteristic scent lowered the preference, and in the case of mixing with MX2, it can be seen that the taste of the juniper was added and the preference was further lowered. In addition, in the case of MX5 mixing, the astringent and sour scent is stronger, so it can be seen that the preference is lower. On the other hand, in the case of the complex extract MX4, it was confirmed that the palatability increased as the aroma and taste peculiar to the noganju extract were neutralized.

Therefore, in the case of the complex extract MX4, it can be provided as a functional food effective in improving muscle function with a more palatable flavor and taste.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

Claims (7)

Containing Juniperus rigida extract
Composition for improving muscle function. The method of claim 1,
The improvement of muscle function is to treat or prevent a disease selected from the group consisting of muscular atrophy, muscular dystrophy, sacopenia, myasthenia, and muscle degeneration.
Composition for improving muscle function. The method of claim 1,
The composition is any one selected from the group consisting of Pittosporum tobira Ait. extract, Cinnamomum japonicum Sieb. extract, Caesalpinia decapetala extract, Smilax chine L. extract, and mixtures thereof. Further comprising
Composition for improving muscle function. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohol and mixtures thereof
Composition for improving muscle function. Functional food comprising the composition for improving muscle function according to any one of claims 1 to 4. An external preparation for skin comprising the composition for improving muscle function according to any one of claims 1 to 4. A pharmaceutical product comprising the composition for improving muscle function according to any one of claims 1 to 4.

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