KR20200095027A - Composition for removing elderly smell and 2-nonenal comprising extract of pepper - Google Patents

Abstract

The present invention relates to a composition for removing an elderly smell and nonenal, containing a pepper extract. The composition for removing the elderly smell, containing the red pepper extract, a persimmon leaf extract, a green tea extract, and a eucalyptus extract according to the present invention can directly remove active oxygen species (ABTS radicals) and inhibit DNA damage caused by oxidative stress. In addition, the composition for removing the elderly smell may exhibit a deodorizing effect on the elderly smell by removing nonenal, which is an aldehyde substance which is a major cause of the elderly smell. The composition for removing the elderly smell contains: the pepper extract; and equal to or more than one extract selected from the group consisting of the persimmon leaf extract, the green tea extract, and the eucalyptus extract.

Description

Composition for removing elderly smell and 2-nonenal comprising extract of pepper}

The present invention relates to a composition for removing senile odor and nonenal containing pepper extract.

Recently, Korea has been constantly in demand for various elderly etiquette products such as beauty and personal health care products for the elderly and neighbors suffering from the peculiar smell of the elderly accompanying the aging phenomenon due to the increase in the elderly population ahead of the entry of the elderly society. .

In old age, cis-9-hexadecenoic acid (C ₁₆ H ₃₀ O ₂ ), a fatty acid contained in sebum, is oxidized and decomposed, resulting in a characteristic senile odor. It is an unsaturated aldehyde material called 2-nonenal (C ₉ H ₁₆ O), and 3-hexyl-2-propenal, 3-hexylacrolein, and heptylidene Acetaldehyde (heptylideneacetaldehyde), beta-hexylacrolein (β-hexylacrolein), alpha-nonenyl aldehyde (α-nonenyl aldehyde), nonylenic aldehyde or trans-2-nonenal (trans-2-nonenal) Also called). Although 2-nonenal is produced mainly in the elderly, it can also occur in patients with chronic diseases, especially in patients with limited mobility. As the metabolism is not smooth, more waste products remain on the skin, and trans-2-nonenal, a substance created by the decomposition of unsaturated fatty acids in these sebaceous glands, is made, resulting in a pungent odor. Therefore, it is expected that preventing the oxidation of cis-9-hexadecenoic acid, a reactant discharged from the body through the sebaceous glands, will suppress senile odor, and by removing the nonenal component itself, which is the main cause of senile odor, Research is also being conducted to deodorize the smell.

Oxygen is the most important molecule indispensable for maintaining life. For example, brain cells begin to be destroyed if oxygen is not supplied for only 30 seconds. The organ that acts as a key plant that creates energy in the cell is the'mitochondria'. Oxygen carried to the mitochondria produces energy by breaking down glucose made by human intake of nutrients (carbohydrates, proteins, fats). Water, carbon dioxide, and oxygen are generated in the energy production process, and this oxygen is called a reactive oxygen species (ROS). Reactive oxygen species are produced during normal intracellular activity and are involved in a variety of biological processes including cell differentiation, gene expression, and response to cytokines. Oxidative stress is a phenomenon in which ROS in cells increases due to an imbalance between the production of ROS and the antioxidant reaction that removes it, reacts with DNA, proteins, and lipids, causing damage. This is known as a key cause of aging and heart-related diseases. .

Most reactive oxygen species are produced as by-products between mitochondrial electron transport. In addition, ROS forms an intermediate necessary for a metal catalytic oxidation reaction. The oxygen atom has two negative electrons in different orbits of the outer electron shell. This electronic structure makes oxygen susceptible to radical formation. The sequential degeneration of oxygen by the addition of electrons leads to the formation of ROS such as superoxide, hydrogen peroxide, hydroxyl radical, hydroxyl ion, and nitric oxide.

Detoxification of reactive oxygen species is of paramount importance for the survival of all aerobic organisms. Many of the defense mechanisms commonly referred to have evolved to meet this need, providing a balance between the elimination and creation of ROS. The imbalance of ROS production and removal is referred to as oxidative stress. Cells have a variety of defense mechanisms to ameliorate the harmful effects of ROS. Superoxide dismutase (SOD) catalyzes the conversion of two superoxide anions inserted into hydrogen peroxide (H ₂ O ₂ ) and oxygen (O ₂ ) molecules.

2O ₂ + 2H → H ₂ O ₂ + O ₂

In the peroxidation body of eukaryotic cells, the catalase enzyme converts H ₂ O ₂ into water and oxygen, and completes the detoxification of free radicals initiated from SOD.

2H ₂ O ₂ → 2H ₂ O + O ₂

There are many non-enzymatic small molecule antioxidants that play an important role in this detoxification. Glutathione (Glutathione) plays the most important intracellular defense role against the harmful effects of reactive oxygen species. The reaction with the ROS molecule oxidizes Glutathione, but the reduced form is regenerated in redox by the NADPH-dependent reductase. Reduced glutathione (GSH), which is a reduced form of glutathione (GSSG), is a dynamic indicator of oxidative stress in organisms.

On the other hand, pepper ( Capsicum spp . ) is native to South America and has been cultivated for a long time in the Americas. It is widely cultivated from tropical to temperate zones, and in tropical regions it is a perennial plant. It seems that it came to Korea at about the same time as cigarettes, and had a great influence on the dietary life of Koreans.

Persimmon ( Diospyros kaki ) belongs to the persimmon family persimmon tree, and most of the diospyros plants are known to be distributed over 700 species in tropical and subtropical regions. The efficacy of persimmon is effective against hangover detoxification, enuresis, intestinal function enhancement, fever and geothermal action, cough, and chronic bronchitis. Persimmon leaves are effective against gastric ulcer bleeding, scurvy, anemia, heart disease, arteriosclerosis, cerebral hemorrhage, cholesterol lowering, aging. It is known to be effective in inhibiting action, preventing hypertension, diabetes, alcohol detoxification, insomnia and prolapse.

Green tea ( Camellia sinensis O. Kuntze) is a representative plant that is widely consumed as a tea with dried leaves. It is known for its antioxidant effect, cholesterol and blood sugar lowering effect, caries prevention effect, and anticancer effect.

Eucalyptus ( Eucalyptus globulus ) is a tree mainly planted as a plantation tree, and wood is used as a building material or as a tool material. As trees grow, they absorb a lot of moisture, so plant them near your home and use them to dry the surroundings. Fresh leaves are fragrant and volatile eucalyptus is collected and used as medicine. Essential oils have a strong sterilizing effect, so they are also used as bath agents, skin disorders, and insecticides.

Sophora flavescens Solander ex Aiton ) is a plant called'the thief's wand', and is mainly used as a medicinal material by removing the root of Gosam as it is, and is prescribed for indigestion, neuralgia, hepatitis, jaundice, and hemorrhoids. In the private sector, stems and leaves are decoctioned and used as insecticides.

Accordingly, the present inventors have used an extract of Eumseong pepper widely used in Korea to develop an odor remover that can be formulated into a wide range of beauty products and personal health care products without having side effects for health and hygiene, and the pepper extract The present invention was completed by confirming the antioxidant efficacy of and the effect of removing nonenal, which is the main cause of senile odor.

Korean Patent Publication No. 10-2016-0061875

An object of the present invention is to provide a composition for removing senile odor and a composition for antioxidant.

In order to achieve the above object,

The present invention is pepper extract; And it provides a composition for removing senile odor containing one or more extracts selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

In addition, the present invention pepper extract; And it provides a cosmetic composition for removing senile odor containing one or more extracts selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

Further, the present invention is pepper extract; And it provides an antioxidant composition containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

The composition for removing senile odor containing red pepper extract, persimmon leaf extract, green tea extract, and eucalyptus extract according to the present invention can directly remove active oxygen species (ABTS radical) and inhibit DNA damage caused by oxidative stress, as well as senile By removing nonenal, an aldehyde substance that is the main cause of odor, it can exhibit a deodorizing effect on senile odor.

1 is a graph showing the result of measuring ABTS radical scavenging activity (%) according to the treatment concentration (%) of a composition for removing senile odor according to the present invention (Example 2-1).
2 is a graph showing the protective effect against oxidative DNA damage according to the treatment concentration (%) of the composition for removing senile odor according to the present invention (Example 2-1).
3 is a graph showing the effect of removing trans-2-nonenal of the composition for removing senile odor according to the present invention (Example 2-1); Ethanol treatment group (CON), trans-2-nonenal treatment group (NC) and trans-2-nonenal + Example 2-1 treatment group (product).

Hereinafter, the present invention will be described in detail.

Composition for removing senile odor

The present invention is pepper extract; And

It relates to a composition for removing senile odor containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

In the composition for removing senile odor according to the present invention, each containing 0.1 to 1.0% by weight of at least one extract selected from the group consisting of pepper extract, persimmon leaf extract, green tea extract, and eucalyptus extract based on the total weight of the composition for removing senile odor It may be, preferably each containing 0.3 to 0.7% by weight, and more preferably each containing 0.4 to 0.6% by weight.

The composition for removing senile odor according to the present invention may further include a Gosam extract, and may include 0.1 to 1.0% by weight of Gosam extract based on the total weight of the composition for removing senile odor, and preferably 0.3 It may contain to 0.7% by weight, and more preferably may be to contain 0.4 to 0.6% by weight.

The composition for removing senile odor according to the present invention may further include phytoncide, and may include 0.1 to 1.0% by weight of phytoncide based on the total weight of the composition for removing senile odor, preferably 0.3 to 0.7 It may contain weight %, and more preferably contain 0.4 to 0.6 weight %.

The composition for removing senile odor according to the present invention may preferably contain pepper extract, persimmon leaf extract, green tea extract and eucalyptus extract, and more preferably pepper extract, persimmon leaf extract, green tea extract, eucalyptus extract and sophora extract. It may include, and more preferably, pepper extract, persimmon leaf extract, green tea extract, eucalyptus extract, gosam extract, and phytoncide may be included. In this case, each of the extracts and phytoncide may be contained in an amount of 0.1 to 1.0% by weight, preferably 0.3 to 0.7% by weight, and more preferably 0.4 to 0.6% by weight based on the total weight of the composition for removing senile odor. It may contain weight percent.

In the present invention, the red pepper may be dried and used only the peel of the domestic red pepper ( Capsicum spp . ) variety of'Palkesta' in Eumseong-gun, Chungcheongbuk-do.

The term "extract" as used in the present invention refers to a preparation obtained by evaporating the leachate and concentrating the raw material to be extracted as it is or pulverizing it as a leach using an appropriate solvent, but is not limited thereto. It may be an extract obtained by, a diluted solution or a concentrate of the extract, a dried product obtained by drying the extract, and a crude product or purified product thereof. The extract can be prepared using a general extraction method, separation and purification method known in the art. The extraction method is, but not limited thereto, preferably solvent extraction, steam distillation, compression, oil absorption, volatile organic solvent extraction, or supercritical fluid extraction, hot water extraction, hot water extraction, cold sediment extraction, reflux cooling extraction, or ultrasonic wave A method such as extraction may be used, and preferably, a method of solvent extraction or steam distillation may be used. At this time, any suitable solvent that can be used to obtain the extract may be used as long as it is an acceptable solvent in the art, and water or an organic solvent may be used. For example, alcohol having 1 to 4 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether , Benzene, chloroform, ethyl acetate, methylene chloride, hexane, and various solvents such as cyclohexane may be used alone or in combination. It is not limited.

Preferably, the pepper extract, persimmon leaf extract, green tea extract, eucalyptus extract and gosam extract are each selected from the group consisting of water, anhydrous or hydrated lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, hydrocarbon-based solvents, or mixed solvents thereof. It may be extracted using one or more extraction solvents, more preferably water or one or more extraction solvents selected from anhydrous or hydrated lower alcohols having 1 to 4 carbon atoms.

In the composition for removing senile odor according to the present invention, the causative agent of the senile odor may be one or more selected from the group consisting of nonenal, octanal, nonnal, and decanal, preferably nonenal (trans -2-nonenal).

The composition for removing senile odor according to the present invention may effectively act to deodorize senile odor by removing nonenal, which is a major cause of senile odor. The removal of nonenal may be that the composition according to the present invention reacts with nonenal to form or neutralize new molecules that exhibit an unpleasant odor to reduce the concentration of nonenal, resulting in an effect of removing nonenal. It may be to indicate.

In one embodiment of the present invention, the composition for removing senile odor according to the present invention is made of nonenal, which is an aldehyde-based substance that causes senile odor than other aldehyde-based substances that cause odors generated in wastewater, sewage, manure, air conditioners, etc. It was confirmed that the concentration was specifically reduced (see Experimental Example 3).

Therefore, the composition for removing senile odor according to the present invention can also be used as a composition for removing nonenal.

The composition for removing senile odor according to the present invention is not limited in its formulation, and any formulation can be used as long as it can be used as a deodorant, for example, aerosol, stick, roll-on (Roll-On), Pump, Sheet, Patch, Solution, Suspension, Emulsion, Cream, Lotion, Gel, Powder, Soap, And liquid detergents. Among them, the aerosol or pump type is the most widely used formulation because it is easy to use and has excellent feeling of use, but it is also possible to apply the composition for removing senile odor of the present invention to other commonly manufactured deodorant formulations.

For removing aged odor Cosmetic Composition

In addition, the present invention pepper extract; And

It provides a cosmetic composition for removing senile odor containing one or more extracts selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

In the cosmetic composition according to the present invention, it may contain 0.1 to 1.0% by weight, respectively, of one or more extracts selected from the group consisting of pepper extract and persimmon leaf extract, green tea extract, and eucalyptus extract based on the total weight of the cosmetic composition. For example, it may be one containing 0.3 to 0.7% by weight, more preferably each containing 0.4 to 0.6% by weight.

The cosmetic composition according to the present invention may further include a Gosam extract, may include 0.1 to 1.0% by weight of Gosam extract, preferably 0.3 to 0.7% by weight based on the total weight of the cosmetic composition. It may be, and more preferably, it may be one containing 0.4 to 0.6% by weight.

The cosmetic composition according to the present invention may further contain phytoncide, may contain 0.1 to 1.0% by weight of phytoncide, and preferably contain 0.3 to 0.7% by weight, based on the total weight of the cosmetic composition. And, more preferably, it may be one containing 0.4 to 0.6% by weight.

The cosmetic composition according to the present invention is a paste, gel, cream, lotion, powder, solid soap, water soap, shampoo, rinse, solution, suspension, emulsion, mineral cosmetic, powder perfume, surfactant-containing cleansing or surfactant-free cleansing , Oil, powder foundation, emulsion foundation, hair dye, wax, flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, cleansing cream, cleansing foam, pack, pack base, eye cream, fragrance, ointment, cleansing water, powder , It may be one or more formulations selected from the group consisting of wax foundation and spray.

Other ingredients other than the above ingredients may be appropriately selected and blended by a person skilled in the art without difficulty according to other formulations or purposes of use.

In addition, the cosmetic composition may include fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles or commonly used in cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are made. These adjuvants are introduced in amounts commonly used in the field of cosmetology.

When using the cosmetic composition according to the present invention as an external application for skin, additionally fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, interfacial agents Activators, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or external preparations for skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used. In addition, the ingredients can be introduced in an amount commonly used in the field of skin science.

Antioxidant composition

In addition, the present invention pepper extract; And

It provides an antioxidant composition containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract, and eucalyptus extract.

The antioxidant composition according to the present invention can be used as a pharmaceutical, pharmaceutical, food and cosmetic composition.

In a specific aspect of the composition according to the present invention, the antioxidant composition may be used as a pharmaceutical composition. It can be used as a pharmaceutical composition for the prevention or treatment of diseases caused by oxides produced by free radicals, specifically cancer, arteriosclerosis, diabetes, stroke, myocardial infarction, hepatitis, nephritis, atopy, Parkinson's disease, It is effective in preventing or treating diseases caused by ultraviolet rays and radiation. The pharmaceutical composition may be administered through various routes including oral, transdermal, subcutaneous, intravenous, or intramuscular during clinical administration, and may be applied as an external preparation for skin. In addition, it can be formulated into various formulations, and when formulated, it can be formulated using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules and capsules, and such solid preparations contain at least one excipient such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. It is prepared by mixing. Liquid preparations for oral administration include suspending agents, intravenous solutions, emulsions and syrups, and other excipients, such as wetting agents, wetting agents, sweeteners, fragrances and preservatives, in addition to water and liquid paraffin, which are commonly used simple diluents. Can be included. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, Witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerol, gelatin, and the like may be used.

When using the composition of the present invention as a food additive, the pepper extract; And one or more extracts selected from the group consisting of persimmon leaf extract, green tea extract, and eucalyptus extract may be added as it is or may be used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment), and may further include food additives acceptable for food.

The food composition of the present invention may include all foods in a conventional sense, and may be mixed with terms known in the art, such as functional foods and health functional foods.

The term "functional food" of the present invention refers to a food manufactured and processed using raw materials or ingredients having functions useful for the human body according to the Health Functional Food Act No. 6727, and the term "functional" refers to the structure of the human body. And it means ingestion for the purpose of obtaining useful effects for health use such as controlling nutrients for function or physiological action.

In addition, the term "health functional food" of the present invention refers to a food manufactured and processed by extracting, concentrating, refining, mixing, or extracting, concentrating, refining, or mixing a specific ingredient contained in a food ingredient as a raw material for the purpose of health assistance, It refers to foods designed and processed to sufficiently exert biological control functions such as biological defense, biological rhythm control, disease prevention and recovery, etc. by the above ingredients. The health food composition is It can perform functions related to recovery, etc.

There is no limitation on the kind of food in which the composition of the present invention can be used. In addition, pepper extract of the present invention; And the antioxidant composition containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract, and eucalyptus extract may be prepared by mixing suitable other auxiliary ingredients and known additives that may be contained in food at the choice of those skilled in the art. have. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding the extract according to the present invention as a main component, such as juice, tea, jelly, and juice.

In addition, foods that can be applied to the present invention include, for example, special nutritional foods (e.g., formulas, infant foods, etc.), processed meat products, fish meat products, tofu, rice cakes, noodles (e.g., ramen, noodles, etc.), health supplement foods. , Seasoned foods (e.g. soy sauce, miso, red pepper paste, mixed sauce, etc.), sauces, sweets (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.) ), beverages (e.g. fruit, vegetable beverages, soy milk, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.).

When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweetening agents, flavoring agents, natural carbohydrates, and the like as an additional component, like a normal beverage. In addition to the above, the health functional food composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin. , Alcohols, carbonated beverages, and the like. In addition, it may contain flesh for the manufacture of natural fruit juice, fruit juice beverage and vegetable beverage.

Hereinafter, the present invention will be described in more detail by examples. These examples are merely for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited to these examples.

< Example 1> Red pepper, persimmon leaves, green tea, eucalyptus and Gosam Preparation of extract

The seeds of “PR Keista (Pr Keeta, Korea Dakii Co., Ltd.),” a domestic pepper variety in Eumseong-gun, North Chungcheong Province, were discarded, and only the peel was separated and air dried at 40°C. The dried red pepper peel was cut into 2-3 cm, and then pulverized to a size of 3 mm or less.

A hot water extract of hot pepper was obtained by reflux extraction at 40° C. for 2 hours with distilled water having a volume corresponding to 30 times (wt/v%) of the pulverized red pepper peel powder. The extract was concentrated under reduced pressure at 50°C or less using a rotary evaporator (Ika, China), and the concentrated solution was freeze-dried at -80°C to prepare a final red pepper extract. The final yield (yield) of the red pepper extract is 56.9%.

In addition, persimmon leaf extract, green tea extract, eucalyptus extract, and gosam extract were prepared from persimmon leaf, green tea (leaf), eucalyptus (leaf), and gosam (root) powder in the same manner as the pepper extract preparation. At this time, persimmon leaves and green tea leaves were purchased domestically from Jecheon Herb, and eucalyptus leaves were used by purchasing Portuguese acid from Herb Village Co., Ltd.

< Example 2> Preparation of composition for removing senile odor

The red pepper extract, persimmon leaf extract, green tea extract, eucalyptus extract, and gosam extract prepared in Example 1 were mixed in the ratio of Table 1 to prepare a composition for removing senile odor. At this time, the phytoncide essential oil of Table 1 below was used by purchasing 100% phytoncide essential oil from Myeongeuldam.

Unit: wt% Example 2-1 Example 2-2 A Deionization water 75.75 75.25 Ethanol (Et-OH) 20.00 20.00 TW-80 0.75 0.75 B Phytoncide essential oil 1.50 1.50 C Pepper extract 0.50 0.50 Persimmon leaf extract 0.50 0.50 Green tea extract 0.50 0.50 Eucalyptus extract 0.50 0.50 Sophora extract - 0.50

< Reference example > Statistical processing

The data of the following experimental examples were presented as mean±standard deviation (SDM). All experiments were repeated three times. A one-way analysis of variance (ANOVA) test was performed to compare the measured values between each group, and the difference was verified at a significance level of p <0.05. GraphPad Prism 5 and Microsoft Excel 2007 were used for graph evaluation and statistics.

< Experimental Example 1> ABTS Analysis of radical scavenging activity

The composition for removing senile odor of Example 2-1 was diluted with distilled water (DW) at concentrations of 100, 50, 25, 12.5 and 6.25% to prepare 1 ml each, and as a positive control (PC) standard 1 ml was prepared by diluting the used ascorbic acid with distilled water to a concentration of 25%. After mixing 7mM ABTS dissolved in distilled water and 2.45mM Potassium persulphate, it was left for 12-16 hours in the dark for stabilization. This solution (ABTS+solution) can be used for 2 days at room temperature. The mixture was diluted with PBS (0.01M, pH 7.4) so that it became 0.70±0.02 at 734 nm. Then, the diluted senile odor removal composition and ascorbic acid were mixed with 0.8 ml of ABTS+ solution in 0.2 ml each, reacted for 5 minutes at room temperature, and then measured at 734 nm for the blank. As a control, a solvent was used instead of the sample of Example 2-1, and the ABTS radical scavenging activity (%) was calculated as follows and shown in FIG. 2.

Scavenging activity(%) = (1-Absorbance of sample / Absorbance of control) × 100

As a result, as shown in FIG. 1, it was confirmed that the composition for removing senile odor of Example 2-1 has an excellent ABTS radical activity inhibitory effect compared to ascorbic acid (AA), which is a positive control (PC).

< Experimental Example 2> DNA Active protection against damage analysis

Prevention of oxidative damage to DNA by H ₂ O ₂ was performed by slightly modifying the experimental method of Tian & Hua (2005). 1 μL of plasmid PBR 322 DNA (0.5 μg/μL) contains ₃ μL of distilled water containing ₂ μL (0.08 mM) of FeSO ₄ (0.08 mM) and ₃ μL of 30% (v/v) H ₂ O ₂ and different concentrations (25, 12.5 and 6.25%) A mixture of 2 μL of the composition for removing senile odor of Example 2-1 was reacted at 37° C. for 1 hour. After that, 2 μL of 6X DNA loading dye was added. It was reacted to 0.8% agarose gel electrophoresis of 70V at room temperature. DNA bands (supercoiled, linear and open circular) were stained with ethidium bromide, and the effect of inhibiting DNA oxidation was evaluated by increasing and decreasing the supercoiled monomer based on the control value.

As a result of the protective effect of the composition for removing senile odor of Example 2-1 on DNA damage, as shown in FIG. 2, a group causing oxidative damage compared to the plasmid DNA control (Con) (Damage group, Dam) In, supercoiled DNA was completely transformed into a linear form by DNA damage. On the other hand, when the composition for removing senile odor of Example 2-1 was treated, the DNA of Supercoiled was restored in a dose-dependent manner. From these results, it was confirmed that all of the compositions for removing senile odor of Example 2-1 exhibited a DNA protection activity against DNA damage.

< Experimental Example 3> Nonenal Evaluating the effectiveness of removal

The experiment was conducted as follows to evaluate the effect of removing nonenal, an aldehyde substance that is the main cause of senile odor. Specifically, 25 µl of trans-2-nonenal was treated with 475 µl of the composition for removing senile odor, stirred for 3 hours, treated with 5 ml of ethanol, and then Pelling Solution I 150 µl, Pelling Solution II was added to 150 µl (equal amount mixing) and reacted for 15 minutes at 60°C, and the absorbance was measured.

3 is a result of a Pelling reaction by treating an elderly odor removal composition with trans-2-nonenal. Control is the pelleting reaction by treating only pure ethanol, the product is the pelleting reaction by treating the old odor removal composition in trans-2-nonenal, and the peeling reaction by treating only trans-2-nonenal in NC. It is a result showing the trans-2-nonenal activity (%) by calculating the degree of absorption compared to NC.

As shown in FIG. 3, when the composition for removing senile odor of Example 2-1 was treated, the concentration of trans-2-nonenal was decreased, and it was confirmed that senile odor can be effectively removed.

In addition, in the same manner as above, the effect of removing nonenal (=α-nonenyl aldehyde, trans-2-nonenal, trans-2-nonenal) of the composition for removing senile odor of Example 2-2 was confirmed, and , Acetaldehyde (C ₂ H ₄ O; CH ₃ CHO), propionaldehyde (C ₃ H ₆ O; CH ₃ CH ₂ CHO), n-part, known as an aldehyde-based malodorous substance generated from wastewater, sewage, manure, air conditioner, etc. Tyraldehyde (C ₄ H ₈ O; CH ₃ (CH ₂ ) ₂ CHO) iso-valeraldehyde (C ₅ H ₁₀ O; (CH ₃ ) ₂ CHCH ₂ CHO) or n-valeraldehyde (C ₅ H ₁₀ O; CH ₃ (CH ₂ ) ₃ CHO) was treated with the compositions for removing senile odors of Examples 2-1 and 2-2, respectively, and the results of measuring the aldehyde concentration (%) through a Pelling reaction are shown in Table 2 below.

density(%) Nonenal Acetaldehyde Propion aldehyde n-butyraldehyde iso-valer aldehyde n-valer aldehyde Aldehyde 100 100 100 100 100 100 Aldehyde + Example 2-1 64.2 89.4 88.4 87.6 87.4 86.2 Aldehyde + Example 2-2 53.4 87.2 86.6 86.4 86.7 85.9

As a result, as shown in Table 2, it was confirmed that the composition for removing senile odor of Examples 2-1 and 2-2 according to the present invention specifically reduced the concentration of nonenal than other aldehyde-based odor sources. I did.

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (13)

Pepper extract; And
A composition for removing senile odor containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract. The method of claim 1,
A composition for removing senile odor, comprising 0.1 to 1.0% by weight of at least one extract selected from the group consisting of pepper extract, persimmon leaf extract, green tea extract, and eucalyptus extract based on the total weight of the composition for removing senile odor The method of claim 1,
The composition for removing senile odor is a composition for removing senile odor, characterized in that it further comprises a Gosam extract. The method of claim 1,
The composition for removing senile odor is a composition for removing senile odor, characterized in that it further comprises phytoncide. The method of claim 1,
The causative agent of the senile odor is a composition for removing senile odor, characterized in that at least one selected from the group consisting of nonenal, octanal, nonnal and decanal. The method of claim 5,
A composition for removing senile odor, characterized in that the causative agent of the senile odor is trans-2-nonenal. Pepper extract; And
A cosmetic composition for removing senile odor containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract. The method of claim 7,
A composition for removing senile odor, comprising 0.1 to 1.0% by weight of at least one extract selected from the group consisting of red pepper extract and persimmon leaf extract, green tea extract, and eucalyptus extract based on the total weight of the cosmetic composition. The method of claim 7,
The cosmetic composition is a composition for removing senile odor, characterized in that it further comprises a Gosam extract. The method of claim 7,
The cosmetic composition is a composition for removing senile odor, characterized in that it further comprises phytoncide. The method of claim 7,
The cosmetic composition is a cosmetic composition for removing senile odor, characterized in that it has antioxidant activity. The method of claim 7,
The cosmetic composition is a paste, gel, cream, lotion, powder, solid soap, water soap, shampoo, rinse, solution, suspension, emulsion, mineral cosmetic, powder perfume, surfactant-containing cleansing or surfactant-free cleansing, oil, Powder foundation, emulsion foundation, hair dye, wax, flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, cleansing cream, cleansing foam, pack, pack base, eye cream, fragrance, ointment, cleansing water, powder, wax foundation And a cosmetic composition for removing senile odor, characterized in that at least one formulation selected from the group consisting of spray. Pepper extract; And
Antioxidant composition containing at least one extract selected from the group consisting of persimmon leaf extract, green tea extract and eucalyptus extract.

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