KR20200094533A - Composition for antioxidant and fatigue recovery comprising mixture of wild ginseng sprout, Codonopsis lanceolata sprout and barley sprout extract as effective component - Google Patents

Abstract

The present invention relates to a composition for antioxidant and fatigue recovery composition including the mixture of a wild ginseng sprout extract, a Codonopsis lanceolata sprout extract, and a barley sprout extract as active ingredients. More specifically, the wild ginseng sprout extract, the Codonopsis lanceolata sprout extract, and the barley sprout extract are extracted through hot water extraction and alcohol extraction. Antioxidant activities of the wild ginseng sprout extract, the Codonopsis lanceolata sprout extract, and the barley sprout extract are proved by 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) and 2.2-azino-bis(3-rthylbenzthiazoline-6-sulfonic acid) (ABTS) test methods. Moreover, in a forced swimming test of an animal model injected with the mixture of the wild ginseng sprout extract, the Codonopsis lanceolata sprout extract, and the barley sprout extract, a swimming time of an injected group is greater than that of a comparative group not injected with the same or a positive comparative group (taurine and red ginseng), thereby proving that the composition has significant fatigue recovery effect.

Description

Composition for antioxidant and fatigue recovery comprising mixture of wild ginseng sprout, Codonopsis lanceolata sprout and barley sprout extract as effective component}

The present invention relates to a composition for anti-oxidation and fatigue recovery, which contains a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley as an active ingredient.

Due to the busy work and daily life of modern people, cases of physical and mental fatigue are increasing. In general, fatigue is a condition in which work efficiency decreases when work is performed, a state of confusion of homeostasis, or a state of restraining of the cerebral cortex is defined as'reduction of ability to perform physical and mental activities'. . Therefore, the fatigue phenomenon felt by an individual can be largely divided into mental and physical fatigue. When the signal of fatigue comes, the body must take a break and seek recovery, but in a busy modern society, it is not easy to keep this cycle of fatigue and recovery properly. Accumulation of fatigue caused by overworking can cause chronic fatigue and cause diseases such as peptic ulcer, hypertension and diabetes. For the recovery of fatigue, temporary fatigue recovery effect can be expected through the intake of a fatigue recovery agent along with rest therapy.

Frequently, nutritional tonic alteration agents or fatigue recovery agents are foods or medicines that help the body vitalize by supplying nutrients to the body and release harmful substances to the human body.Taurine, vitamin B, red ginseng, etc. Caffeine anhydride, nicotinamide, etc. are known. However, most nutritious tonicity agents contain caffeine, which is helpful for temporary fatigue recovery, but the side effects are concerned, so it is urgent to develop a fatigue recovery or improvement agent using natural ingredients.

In addition, the demand for functional food related to fatigue recovery is increasing due to the heavy work of modern people.

On the other hand, in general, Korean wild ginseng (Korean panax ginseng) is artificially germinated from natural wild ginseng seeds or seedlings grown as wild as possible close to the growth environment of wild ginseng, and deeply sprayed in the shaded and humid places in the deep mountains and left in the natural environment. After years to decades of neglect after leaving it unattended, it refers to the ginseng, but the exact definition of the wild ginseng remains unclear. In other words, mountain ginseng is also called camphor ginseng, elder, and camphor ginseng. It is said that the thickness and length of the roots, the presence or absence of torso, the number of brainheads according to age, and the lifespan vary depending on soil and climatic conditions, and has a stronger scent than ginseng. According to the analysis results of ginseng and wild ginseng, ginsenosides Rh1 and Rg3 are not found in ginseng, but some small amounts are detected in ginseng wild ginseng. There are reports. Due to this, the wild ginseng is considered to have excellent high added value in terms of efficacy and marketability compared to the saturated ginseng market in terms of market competitiveness, and it is thought that there is an alternative material and commerciality. It has been reported that the effect is increased.

In addition, Deodeok is a perennial vine plant with dicotyledonous plants and campanula flowers. The scientific name is Codonopsis. lanceolata , distributed in Korea, Japan, China, and the Far East of Russia. It is a plant native to moist grasslands, forests, or valleys. It is mainly edible and is also called sasam or white ginseng. The root of Deodeok is thick like a bellflower, and when you cut a plant, white juice appears. Leaves are misaligned, and at the end of short branches, four leaves approach each other and appear to run together, and they are 3-10 cm long and 1.5-4 cm wide, long oval. The leaf edges are flat, the front is green, and the back is white. In August-September, bell-shaped frequent flowers hang from the end of short branches downward. The calyx is sharply divided into 5 pieces, green, 2-5cm long, and 6-10cm butterfly. The corolla is 2.7~3.5cm long, and the ends are divided into 5 pieces, curled backwards, light green on the outside, and purple spots on the inside. Fruits ripen in September. Plant young leaves in spring and roots in autumn.

In addition, Sprout Barley has been known as a food source that has a high nutritional content as well as a high content of inorganic components such as calcium, magnesium, and potassium, and also has antioxidant, anti-inflammatory, and anti-cancer functions. Eggplant contains functional secondary metabolites, such as saponarin, lutonarin, and isovitexin, and the industrial applicability thereof is increasing as a material for health functional foods and pharmaceuticals.

As a prior art related to antioxidant and fatigue recovery, Korean Patent Registration No. 1882468 discloses a method for producing a fermented goat's ginseng lactic acid bacterium that increases Rg3, Rh1, compound K content and antioxidant efficacy by fermentation of separated lactic acid bacteria, and Korean Patent No. 1817053 Antioxidant, anti-cancer, anti-obesity or immune-enhancing composition comprising Hodeuk extract as an active ingredient is disclosed, but a composition for antioxidant and fatigue recovery comprising a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley of the present invention as an active ingredient No disclosure has been made.

The present invention was derived by the above-mentioned needs, the present invention provides a composition for antioxidant and fatigue recovery comprising a mixed extract of sprouted wild ginseng, sprouted sprout and sprouted barley, and sprouted wild ginseng, sprouted sprout and sprouted barley. In the forced swimming test of an animal model, each of which was extracted with hot water and alcohol, and confirmed the antioxidant activity of the sprouted wild ginseng, sprouted deer and sprouted barley extracts by DPPH and ABTS test methods, and the mixed extract of sprouted wild ginseng, sprouted deer and sprouted barley was administered. , The present invention was completed by confirming that there is a remarkable fatigue recovery effect from that the swimming time is longer than that of the control or positive control group (taurine and red ginseng) in which the mixed extract of the present invention was not administered.

In order to achieve the above object, the present invention provides a health functional food composition for antioxidant and fatigue recovery, which includes a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley.

In addition, the present invention provides a method for recovering fatigue comprising administering the composition to a non-human subject.

The present invention is derived by the above-mentioned needs, the present invention relates to a composition for antioxidant and fatigue recovery comprising a mixed extract of sprouted wild ginseng, sprouted sprout and sprouted barley, sprouted wild ginseng, sprouted sprout and sprouted barley extract This antioxidant activity is excellent, and in the forced swimming test of the animal model to which the mixed extract of sprouted ginseng, sprouted sprout and sprouted barley was not administered, the control group containing the mixed extract of sprouted wild ginseng, sprouted sprout and sprouted barley according to the present invention, or It was confirmed that there was a significant fatigue recovery effect from the longer swimming time compared to the positive control group (taurine and red ginseng).

1 is a result of measuring the swimming time by the forced swimming test method after administering the mixed extract of sprouts ginseng, sprouts and ducks of the present invention for 2 weeks. A is a negative control group, nothing is administered, B is a group in which 10 mg/kg of a complex extract containing a mixed extract of sprouted ginseng, sprouted duck and sprouted barley according to the present invention, and C is sprouted wild ginseng according to the present invention , 30 mg/kg of the complex extract containing the mixed extract of sprout and sprout barley, D is the group of 100 mg/kg of the complex extract containing the mixed extract of sprout red ginseng, sprout sprout and sprout sprout according to the present invention And, E is a group in which 1000 mg/kg of a compound extract containing a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley according to the present invention is administered, F is a positive control group, and 1000 mg/kg of taurine is administered, and G is positive. As a control group, red ginseng was administered at 200 mg/kg. *, **, *** is a group in which 100 or 1000 mg/kg of a complex extract containing a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley according to the present invention is compared to a negative control group, and 1000 mg/kg of the positive control taurine The forced swimming time of the administered group was statistically significantly increased. * means p<0.05, ** means p<0.01, and *** means p<0.001.

The present invention relates to a health functional food composition for anti-oxidation and fatigue recovery, which contains a mixed extract of sprouts ginseng, sprouts, and sprouts as an active ingredient.

The sprouted wild ginseng, sprouted duck and sprouted barley mixed extract may be prepared by a method including the following steps, but is not limited thereto:

(1) a step of extracting each of the extracting solvent to each of the sprouted wild ginseng, sprouted duck or sprouted barley dry powder;

(2) filtering the sprouted wild ginseng, sprouted duck or sprouted barley extract obtained in step (1); And

(3) The step of preparing the extract by concentrating and drying the filtered sprouted wild ginseng, sprouted duck or sprouted barley extract in step (2) under reduced pressure.

In the step (1), the extraction solvent is preferably selected from water, a lower alcohol of C ₁ to C ₄ or a mixture thereof, more preferably water or ethanol, but is not limited thereto.

In the above manufacturing method, any conventional methods known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, may be used as the extraction method. The extraction solvent is preferably extracted by adding 1 to 20 times the weight of dried sprouts ginseng, sprouts, and sprouts barley, more preferably 5 to 15 times. The extraction temperature is preferably 4 to 100°C, but is not limited thereto. In addition, the extraction time is preferably 0.5 to 10 hours, more preferably 0.5 to 6 hours, and most preferably 4 hours, but is not limited thereto. In the above method, the vacuum concentration in step (3) is preferably a vacuum reduced pressure concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably dried under reduced pressure, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

The fatigue is characterized by fatigue caused by muscle fatigue or stress caused by exercise, and muscle fatigue may be manifested as a feeling of fatigue, endurance, improvisation, or lethargy.

Health functional food composition for fatigue recovery according to an embodiment of the present invention is dried Omija, bokbunja fruit, goji berry fruit, bee-box fruit, Cornus officinalis, roasted maca powder, geonji hwang, ginseng ginseng seeds, ginseng root, in addition to the active ingredients It may further include one or more selected from zinc oxide, L-arginine, terrestrial trilobite, and twin cricket.

The composition is preferably prepared in any one formulation selected from powders, granules, pills, tablets, capsules, candy, syrups and beverages, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method. The mixing amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). In general, the health functional food composition of the present invention in the manufacture of food or beverage is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There are no particular restrictions on the type of the health functional food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum products, dairy products including ice cream, various soups, beverages, tea Drinks, alcoholic beverages, and vitamin complexes are included and include all healthy foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients that are commonly added in food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink agent, natural carbohydrates or flavoring agents may be included as additional components in addition to the active ingredient. The natural carbohydrates include monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, etc.), or sugar alcohols ( Xylitol, sorbitol, erythritol, etc.) are preferred. The flavoring agent may be a natural flavoring agent (eg, tau martin, stevia extract, etc.) and a synthetic flavoring agent (eg, saccharin, aspartame, etc.).

Various nutritional supplements, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonic acid It may further contain a carbonation agent used in beverages. The proportion of the components to be added is not particularly important, but it is generally selected from the range of 0.01 to 0.1 parts by weight with respect to 100 parts by weight of the health functional food composition of the present invention.

It also relates to a method for recovering fatigue, comprising administering a composition according to the invention to a non-human subject.

Hereinafter, the present invention will be described in more detail using examples. It is obvious to those skilled in the art that these examples are only intended to illustrate the present invention in more detail and that the scope of the present invention is not limited by them.

Example One. Sprout Mountain Ginseng , Sprout And Preparation of sprout barley extract and Sprout Mountain Ginseng Preparation of composites comprising, sprouts and sprout barley

For each of dry sprouted ginseng, sprouted duck or sprouted barley dry powder, water and ethanol (alcohol) were added as an extraction solvent, extracted, filtered and concentrated to dryness under reduced pressure.

In addition, in order to prepare a complex comprising sprouted wild ginseng, sprouted sprouts, and sprouted barley, dried Schisandra chinensis, Bokbunja fruit, Goji berry fruit, bee-box fruit, Cornus officinalis fruit, geonji-hwang, ginseng seed ginseng seed, ginseng root, trilobite top, twin After mixing cricket, ginseng ginseng ginseng extract, bud deodeok extract and bud barley extract at the ratios shown in Table 3, extracting hot water, preparing a mixed extract through filtration and concentration, and roasting maca powder in the prepared mixed extract , Zinc oxide, L-arginine, and mixed with steamed glutinous rice powder to complete the composite.

Example 2. Sprout Mountain Ginseng , Sprout And antioxidant activity of Sprout barley extract

Antioxidant effect (free radical scavenging rate) using DPPH(2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl) method and ABTS(2.2-azino-bis(3-rthylbenzthiazoline-6-sulfonic acid) method The free radical scavenging (%) was confirmed at a wavelength of 520 nm by comparing the degree of decrease in absorbance due to the reduction of DPPH compared to the untreated group.

In addition, 7 mM ABTS (2.2-azino-bis (3-rthylbenzthiazoline-6-sulfonic acid) and 2.4 mM potassium persulfate) were mixed at a final concentration and allowed to stand for 24 hours in a cow at room temperature to form ABTS+. , Absorbance at 760 nm was measured.

As a result, as disclosed in Tables 1 and 2 below. Antioxidant activity of the sprout sprout, sprout barley and sprout wild ginseng extract was confirmed.

Antioxidative effect of hot water and alcoholic extract of Sprout wild ginseng, Sprout deodeok and Sprout barley, confirmed by DPPH test method material DPPH-SC ₅₀ (µg/ml) material DPPH-SC ₅₀ (µg/ml) Sprout extract 458.67 Sprout Hot Water Extract 789.70 Sprout Barley Extract 836.92 Sprout barley hot water extract 1436.43 Sprout wild ginseng alcohol extract 1268.31 Sprout wild ginseng hot water extract 989.91

Antioxidative effect of hot water and alcoholic extracts of Sprout wild ginseng, Sprout deodeok and Sprout barley confirmed by ABTS test method material ABTS-SC ₅₀ (µg/ml) material ABTS-SC ₅₀ (µg/ml) Sprout extract 690.80 Sprout Hot Water Extract 904.68 Sprout Barley Extract 655.22 Sprout barley hot water extract 961.51 Sprout wild ginseng alcohol extract 793.73 Sprout wild ginseng hot water extract 864.41

Example 3. Sprout Mountain Ginseng , Sprout And the sprouted barley mixed extract, the antioxidant activity of the complex and the forced swimming test of the animal model administered with the complex

A composite comprising a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley of the present invention was prepared (Table 3), and the antioxidant activity of the complex was confirmed (Table 4).

Ingredients and blending ratio of the complex comprising the sprouted wild ginseng, sprouted duck and sprouted barley extract of the present invention No. ingredient Mixing ratio (% by weight) One Dried omija 6 2 Fruit fruit 6 3 Wolfberry fruit 6 4 Bee-box fruit 4 5 Cornus officinalis 8 6 Roasted maca powder 35 7 Guernsey 4 8 Gatsilsaesamsam seeds 4 9 Ginseng root 3 10 Zinc oxide One 11 L-arginine One 12 Trilobite 5 13 Cricket Cricket 4 14 Sprout Mountain Ginseng 0.2 15 Sprout barley 3 16 Sprout 5 17 Steamed rice 4.8

Antioxidant Effect of the Composite Composition Containing Sprout Mountain Ginseng, Sprout Deodeok and Sprout Barley Extracts of the Present Invention material DPPH-SC ₅₀ (µg/ml) ABTS-SC ₅₀ (µg/ml) Composite composition 755.063 729.26

In addition, after administering the prepared complex to the animal model mouse for 2 weeks, forced swimming was performed according to the forced swimming method. At this time, taurine and red ginseng were administered as positive controls.

As a result, as shown in FIG. 1, it was confirmed that the swimming time of the animal model mouse administered with the complex containing the active ingredient of the present invention was significantly increased compared to the negative control without administration of anything.

Claims (5)

A health functional food composition for antioxidant and fatigue recovery, which contains a mixed extract of sprouted wild ginseng, sprouted duck and sprouted barley as an active ingredient. According to claim 1, The sprouts ginseng, sprouts, and sprouts barley mixed extract is water, C ₁ ~ C ₄ lower alcohol or a mixture thereof, and extracted with a solvent, characterized in that the mixed extract and antioxidant and fatigue meeting Taking health functional food composition. According to claim 1, In addition to the active ingredients, dried Schisandra chinensis, bokbunja fruit, goji berry fruit, bee-box fruit, Cornus officinalis fruit, roasted maca powder, dried sage, ginseng ginseng seeds, ginseng root, zinc oxide, L-arginine, trilobite vinegar Health functional food composition for antioxidant and fatigue recovery, characterized in that it further comprises at least one selected from above-ground and twin-star cricket. According to claim 1, The composition is a powder, granules, pills, tablets, capsules, candy, syrup and beverages, health functional food composition for antioxidant and fatigue recovery characterized in that it is prepared in any one formulation selected from. A method for recovering fatigue comprising administering a composition according to claim 1 to a non-human subject.

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