KR20200092015A - An apparatus for sterilizing pathogenic microorganism and a system using thereof - Google Patents

Abstract

An apparatus for sterilizing pathogenic microorganisms is provided. One embodiment includes: a body; a tray installed on the body and loaded with aqueous solutions and samples therein; a driving unit installed on the body and providing rotational vibration motion to the tray; and a light source unit for irradiating sterilizing light to the sample loaded on the tray. Pathogenic microorganisms separated by rotational vibration of the sample can be killed by sterilizing light.

Description

Pathogenic microorganism sterilization device and system using the same{AN APPARATUS FOR STERILIZING PATHOGENIC MICROORGANISM AND A SYSTEM USING THEREOF}

The present invention relates to a pathogenic microorganism sterilizing device and a system using the same.

Food poisoning accidents caused by food pathogenic microorganisms are steadily increasing, and these pathogenic microorganisms are known to cause food poisoning through cross-contamination of food while being present on various surfaces within the food itself or in the cooking and storage environment of food. In addition, there are many biofilm-related infectious diseases in the food contact surfaces, such as stainless steel, plastic, and rubber, which are easily settled by microorganisms to form a biofilm.

On the other hand, high pressure treatment, electric fields treatment, ultrasonic treatment, gaseous sanitizer treatment, acid/base wash liquid to control pathogenic microorganisms present on the surface of such food and utensils to date (acid/alkali wash) treatment, chlorine treatment, and peracid sanitizer treatment have been studied.

However, in the case of electric field, ultrasonic treatment, etc., there is a disadvantage in that the area capable of sterilization is narrow in practical application, and there is a problem that only fixed equipment can be used and installation and processing costs are high. In addition, in the case of a gas sterilizer, there may be a safety problem for workers.

In addition, in the case of various chemical sterilizing disinfectants, residues may be generated after treatment, and may react with organic substances to generate harmful by-products, and may cause corrosion depending on the type of the contact surface. Due to these problems, a washing process requiring a large amount of washing water after chemical sterilization treatment is required, and in this process, pathogenic microorganism re-contamination due to reuse of washing water may occur.

Accordingly, there is a need to develop a sterilization device that can be easily used in food manufacturing processes and food service facilities and has an excellent pathogenic microorganism removal effect, and ultraviolet treatment that has low treatment cost and no harmful byproducts has been spotlighted.

However, despite the many advantages of conventional ultraviolet treatment, there is a disadvantage in that the penetration depth of ultraviolet rays is insufficient to sufficiently remove pathogenic microorganisms present in the gaps between food and utensils.

Therefore, there is a need to develop a sterilization device and a sterilization system capable of effectively sterilizing pathogenic microorganisms by an ultraviolet treatment method.

The problem to be solved by the present invention is to provide a pathogenic microbial sterilization apparatus and a system using the same, which are capable of sterilization at low cost using ultraviolet rays, but can greatly increase the sterilization effect.

In order to solve the above problems, the present invention rotates and vibrates so that the sample is not damaged, and the pathogenic microorganisms propagating between the gaps of the sample surface are detached from the sample surface without damaging the sample, and the detached pathogenic microorganisms are harmful by-products. It is intended to provide a pathogenic microbial sterilization device that can be sterilized efficiently without the occurrence of.

The present invention effectively removes pathogenic microorganisms from the sample surface without damaging the sample, and can effectively sterilize the detached pathogenic microorganisms without generating harmful by-products.

1 shows a sterilizing apparatus for pathogenic microorganisms according to an embodiment of the present invention.
2 shows a driving unit according to an embodiment of the present invention.
Figure 3 shows a tray according to an embodiment of the present invention.
Figure 4 shows a sterilizing device for microorganisms according to another embodiment of the present invention.
5 shows a sterilizing device for microorganisms according to another embodiment of the present invention.
6 is a block diagram schematically showing a sterilization system of a pathogenic microorganism according to an embodiment of the present invention.
7 to 10 show the detection results of pathogenic microorganisms according to Experimental Examples 1 to 4 of the present invention, respectively.

Hereinafter, embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only the present embodiments allow the disclosure of the present invention to be complete and to the person skilled in the art. It is provided to inform you more completely.

When an element is referred to herein as being positioned'above' or'below' another element, this means that the element is located directly above or'below' another element, or there is an additional element between them. It includes all the meanings that can be intervened. In the present specification, the term'upper' or'lower' is a relative concept set from the viewpoint of the observer, and when the viewpoint of the observer changes,'upper' may mean'lower', and'lower' means'upper'. It can also mean.

The same reference numerals in the plurality of drawings refer to elements that are substantially identical to each other. Also, terms such as'include' or'have' are intended to designate the existence of a described feature, number, step, action, component, part, or combination thereof, one or more other features, numbers, or steps. It should be understood that it does not preclude the existence or addition possibility of the operation, components, parts or combinations thereof.

One embodiment of the present invention, the body; A tray installed on the body and loaded with an aqueous solution and a sample therein; A driving unit installed in the body and giving rotational vibration to the tray; And a light source unit for irradiating the sample loaded on the tray with sterilizing light, to kill pathogenic microorganisms detached by rotational vibration of the sample by sterilizing light.

For example, it may further include an emulsifier storage unit for supplying an emulsifier to the tray on which the sample is loaded.

In addition, it may further include a detection unit for detecting pathogenic microorganisms detached from the sample.

Meanwhile, the driving unit may include a driving conversion unit that converts the rotational motion of the driving source into the rotational vibrational motion of the tray.

In another embodiment of the present invention, a system for sterilizing a pathogenic microorganism using the above-described pathogenic microorganism sterilizing apparatus, comprising: an input unit for inputting information of a sample loaded in the tray; A storage unit storing optimized conditions for dissociation of pathogenic microorganisms according to information of a sample input to the input unit; And a control unit for controlling the operation of the driving unit and the light source unit according to the information of the sample input to the input unit, wherein the control unit includes a rotation speed of a driving source included in the driving unit, a light amount of the light source unit, a light irradiation time and a wavelength. It provides a sterilization system of pathogenic microorganisms, which controls at least one of the.

At this time, further comprising an emulsifier storage unit for supplying an emulsifier to the sample loaded on the tray, the control unit can control at least one of the type and amount of the emulsifier input to the tray according to the information of the input sample. .

For example, further comprising a temperature control unit for controlling the temperature inside the tray, the control unit may control the temperature inside the tray according to the information of the input sample.

On the other hand, further comprising a detection unit for detecting pathogenic microorganisms detached from the sample, the control unit to stop the operation of the driving unit, light source unit and emulsifier storage unit when the content of the pathogenic microorganisms detected by the detection unit is less than a preset value Can.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings.

1 shows a sterilizing apparatus for pathogenic microorganisms according to an embodiment of the present invention.

Referring to FIG. 1, a sterilization device for pathogenic microorganisms includes a body 110, a tray 140, a driving unit 120, and a light source unit 160.

The body 110 has a predetermined volume and supports the tray 140 to rotate and vibrate. In the body 110, a driving source (not shown) such as a motor is accommodated therein, and a power supply line for supplying power to the driving unit 120 and the light source unit 160 is accommodated.

The tray 140 is loaded with an aqueous solution and a sample therein, and is installed on the body 110. The tray 140 rotates and vibrates by the driving unit 120 to detach pathogenic microorganisms present on the sample surface from the sample without damaging the sample. For example, the inside of the tray 140 is filled with an aqueous solution, thereby preventing the sample from being damaged when the tray 140 rotates and rotates.

The tray 140 may be installed on the body 110 as shown in FIG. 1. As another example, the tray 140 is installed inside the body 110 so that the body cover (not shown) covers the body 110 so that the tray 140 and the body 110 can be integrally moved. Easy forms can also be applied.

The tray 140 accommodates an aqueous solution and a sample therein, and is loaded on the support plate 135 to receive rotational force and centrifugal force through the support plate 135, and the position is supported by the connecting ring 137 to rotate in place. do. This allows the sample contained therein not to be destroyed and pathogenic microorganisms to be detached from the sample surface.

The tray 140 may have one accommodation space formed therein, and a plurality of trays 140 may be formed therein. The tray 140 having the plurality of accommodation spaces will be described later with reference to FIG. 3.

On the upper surface of the body 110, a drive shaft receiving hole 113 through which the drive shaft 121 of the drive source accommodated therein is exposed to the outside to be coupled with the drive conversion unit 130 is formed through. The drive shaft 121 is protruded to the upper side of the body 110 through the drive shaft accommodation hole 113, and each component of the drive conversion unit 130 is coupled to the protruded drive shaft 121.

In the four corner regions of the upper side of the body 110, a coupling protrusion 111 to which the connecting ring 137 is coupled is provided. The engaging projection 111 is provided to protrude to a certain height from the upper plate surface of the body 110, one end of the connecting ring 137 is coupled. The engaging projection 111 is coupled to one end of the connecting ring 137 to fix the position so that the tray 140 can be rotated and vibrated.

The connecting ring 137 connects the tray 140 and the body 110 to each other so that the tray 140 vibrates and rotates in place from side to side without being idle with the support plate 135. One end of the connecting ring 137 is coupled to the engaging projection 111 of the body 110, and the other end is coupled to the engaging projection 147 of the tray 140. The connecting ring 137 is provided as a member having an elastic force to elastically adjust the gap between the tray 140 and the body 110 so that the tray 140 can be vibrated from side to side.

On the upper end of the engaging projection 111, a detachment preventing projection 111a that holds the position of the connecting ring 137 is coupled so that the coupled link 137 does not escape to the outside due to vibration. The separation preventing protrusion 111a is formed to extend a certain length in the radially outer side of the coupling protrusion 111 so that when the connection ring 137 is about to escape upward due to an external impact, the connection ring 137 is contacted and detached. To prevent. A rubber cover (not shown) may be coupled to the anti-separation protrusion 111a to prevent slipping of the connecting ring 137.

The drive conversion unit 130 is connected to the drive shaft 121 to convert the rotational motion of the drive source to the rotational vibration motion of the tray 140, so that the sample loaded on the tray 140 is not damaged and pathogenic microorganisms are removed from the sample. Tally.

The light source unit 160 irradiates a sterilizing light source, for example, may irradiate ultraviolet rays, and for example, may irradiate a UV-C wavelength of 200 to 280 nm. Since the UV-C wavelength has high energy, it destroys DNA of microorganisms and has high sterilization efficiency. That is, the light source unit 160 may sterilize pathogenic microorganisms detached from the sample surface as a result of the rotational vibration of the tray 140 with a sterilizing light source. In addition, the light source 161 installed in the light source unit 160 is not particularly limited as long as a sterilizing light source such as an LED or an excimer lamp is emitted.

1, the light source unit 160 may be installed to irradiate a sterile light source from top to bottom of the tray 140. Meanwhile, when a form in which the tray 140 is installed inside the body 110 is applied, the light source unit 160 may be installed on the body cover or may be installed on the side of the tray 140 inside the body 110.

The light source unit 160 includes a support frame 151 vertically coupled to the body 110. The support frame 151 adjusts the height at which the light source 161 is supported. The support frame 151 may be provided so that the height is adjustable.

Meanwhile, an emulsifier (not shown) may be supplied to the tray 140 shown in FIG. 1. The emulsifier may be input while the tray 140 is in rotational vibration motion, or may be input in advance before the rotational vibration motion. The emulsifier includes both a hydrophilic group and a hydrophobic group in the molecule, thereby reducing the interfacial tension between the liquid and the solid.

The surface of the sample, for example, the fruit and vegetable, is hydrophobic, and the surface of the pathogenic microorganism is also hydrophobic, so the action of detaching the pathogenic microorganism from the surface of the sample supported in the aqueous solution is not easy. Therefore, the present invention allows the emulsifier having a hydrophilic group and a hydrophobic group in a molecule to be applied to a pathogenic microbial sterilization apparatus, so that the pathogenic microorganisms can be effectively detached from the sample surface.

The type of the emulsifier is an emulsifier having a hydrophilic group and a hydrophobic group in the molecule, for example, polysorbate, acrylate copolymer, trimethylpentanediol/adipic acid/glycerin crosslinked polymer, trimethylpentanediol/adipic acid copolymer, ethyl Hexyl stearate, ethylhexyl salicylate, alkyl methacrylate copolymer, octocrylene, ethylene oxide/propylene oxide block copolymer, and at least one of polyquaternium-101.

For example, the emulsifier is a GRAS (generally recognized as safe) material, including a polysorbate compound, and examples include, but are not limited to, polysorbate 20, 60, 80.

The emulsifier may be applied to 0.1 parts by weight or less per 100 parts by weight of the aqueous solution filled in the tray 140, for example, 0.001 parts by weight to 0.1 parts by weight.

In addition, the emulsifier can be used alone or in combination with the polysorbate compound, sodium lauryl sulfate as a GRAS emulsifier, wherein the sodium lauryl sulfate may be included at 25 ppm to 1000 ppm.

2 shows a driving unit according to an embodiment of the present invention.

Referring to FIG. 2, the driving unit 120 includes a driving conversion unit 130.

The drive conversion unit 130 receives the driving force of the drive source and converts the rotational motion of the drive source into a rotational vibration motion.

The drive conversion unit 130 is disposed in the upper region of the electric shaft 131 and the electric shaft 131 coupled to coincide with the axis of the drive shaft 121 and is disposed eccentrically with a certain length relative to the axis of the drive shaft 121 It includes a conversion plate 133 and a support plate 135 disposed in an upper region of the conversion shaft 133 and supporting the tray 140. The drive converting unit 130 transmits the rotational motion of the driving source to the tray 140 as a rotational vibrational movement by interlocking with the connecting ring 137 connecting the coupling protrusion 111 and the tray 140.

The electric shaft 131 is coupled to the coaxial of the drive shaft 121 and rotates in conjunction with the drive of the drive shaft 121. The electric shaft 131 accommodates the drive shaft 121 protruding outward of the body 110 inside the shaft receiving hole 131a. The electric shaft 131 rotates together when power is applied and the drive shaft 121 is driven, and transmits rotational force to the tray 140.

The conversion shaft 133 is disposed between the transmission shaft 131 and the support plate 135 and is coupled to the drive shaft 121 so as to be eccentric with respect to the axial direction of the drive shaft 121. Accordingly, the conversion shaft 133 rotates eccentrically when the drive shaft 121 is rotated, so that a larger centrifugal force acts on the tray 140 as compared to when it is rotated in the positive axis direction. The centrifugal force acting on the tray 140 acts on the sample so that pathogenic microorganisms can be separated into a diluent.

The support plate 135 supports the tray 140 so that rotational force and centrifugal force are transmitted to the tray 140 through the electric shaft 131 and the conversion shaft 133. The support plate 135 is rotatably coupled to the drive shaft 121 and fixedly coupled to the tray 140. Here, a fixing member (not shown) for fixing the combined state of the two when the tray 140 is loaded on the support plate 135 may be used.

Figure 3 shows a tray according to an embodiment of the present invention.

The tray 140 may be formed to have a plurality of accommodation spaces. The tray 140 is detachably coupled between the tray body 141 in the form of a box in which the upper area is opened, the partition wall 143 partitioning the tray body 141 into a plurality of receiving spaces, and the partition wall 143. It may include a partition 145.

The tray body 141 is made of a plastic material so as to have durability that is not damaged by rotational vibration. For example, a transparent member such as PET can be applied. The partition wall 143 divides the inner space of the tray body 141 into a plurality of spaces. The partition wall 143 may be provided in the form of a cross so that the inner space is divided into a receiving space capable of accommodating four samples, or may be provided in a straight shape capable of accommodating two samples.

In addition, a plurality of coupling grooves 144 to which the partition 145 can be detachably coupled may be provided on the upper side of the partition 143 and the tray body 141 at regular intervals. Thereby, the operator can actively adjust the size of the receiving space by adjusting the coupling position of the partition 145 according to the size of the sample. That is, the tray 140 is provided with a plurality of receiving spaces are provided differently, it can be used in exchange for the support plate 135 according to the number of samples.

The lower region of the tray body 141 is provided with a ring coupling protrusion 147 to which the connecting ring 137 is coupled. The ring engaging projection 147 is coupled to the connecting ring 137 so that the tray 140 does not rotate with the support plate 135 and vibrates from side to side and rotates in place. The ring coupling protrusion 147 is formed to extend a certain length to the lower region of the tray body 141 so that the connection ring 137 is coupled. The lower end portion of the ring coupling projection 147 is provided with a departure preventing projection 147a to prevent the separation of the connecting ring 137.

Figure 4 shows a sterilizing device for microorganisms according to another embodiment of the present invention.

Referring to Figure 4, the sterilizing device of the microorganism further includes an emulsifier storage unit 170.

The emulsifier storage unit 170 supplies the emulsifier to the tray 140 on which the sample is loaded. For example, the emulsifier storage unit 170 may supply different amounts or types of emulsifiers depending on the sample, and the amount or type may be controlled by a control unit described later. The type of the emulsifier may be applied as described above with reference to FIG. 1. In addition, the emulsifier storage unit 170, as shown in FIG. 3, the emulsifier may be classified and stored by type, such as the tray 140 described above, and a single emulsifier or a plurality of emulsifiers of a specific type according to a command signal from a control unit described later Can be put into the tray 140.

5 shows a sterilizing device for microorganisms according to another embodiment of the present invention.

Referring to Figure 5, the sterilizing device of the microorganism further includes a detection unit 180 for detecting pathogenic microorganisms detached from the sample.

The detection unit 180 may detect at least one of the type and amount of pathogenic microorganisms, for example.

Meanwhile, the apparatus for sterilizing microorganisms described above with reference to FIGS. 1 to 5 may further include a display unit (not shown). The display unit may display a driving state of a microbial sterilization device to a user. For example, the display unit, the driving time of the microbial sterilization device, the rotational speed of the driving source, the amount of light, wavelength, light irradiation time of the light source unit 160, the type and amount of emulsifier injected, and the pathogenic microorganisms detected by the detection unit 180 At least one of the type and amount of can be displayed.

In addition, the display unit may include an operation panel (not shown). The operation panel is to enable the user to drive the sterilizing device of pathogenic microorganisms, for example, may include a power button, a timer, a driving control button. The driving control button may control at least one of the rotation time of the driving source, the rotation speed of the driving source, the light amount of the light source unit 160, the wavelength, the light irradiation time, and the type and amount of the emulsifier to be input.

6 is a schematic block diagram showing a sterilization system of pathogenic microorganisms according to an embodiment of the present invention.

6, the sterilization system of pathogenic microorganisms includes an input unit 210, a storage unit 220, a control unit 230, a driving unit 240, and a light source unit 250.

The driving unit 240 and the light source unit 250 may be applied to the above-described bar with reference to FIGS. 1 to 5. In addition, the configuration not shown in FIG. 6 will be described below with reference to FIGS. 1 to 5.

In the input unit 210, information of a sample loaded in a tray (140 in FIG. 1) is input. For example, information on at least one of the type, weight, and volume of the sample may be input.

The storage unit 220 stores the optimized conditions for dissociation of pathogenic microorganisms according to the information of the sample input to the input unit 210 in advance. For example, desorption conditions suitable for the type, weight, and volume conditions of the sample are stored. The desorption condition may include at least one of a rotation time of the driving source, a rotation speed of the driving source, a light amount of the light source unit 160, a wavelength, a light irradiation time, and the type and amount of the emulsifier to be input. For example, the tally condition may be displayed on the display unit.

The control unit 230 controls at least one of the driving unit 240, the light source unit 250, and the emulsifier storage unit (170 in FIG. 4) according to the information of the sample input to the input unit 210. For example, the information of the sample input to the input unit 210 is transferred to the storage unit 220, and the storage unit 220 determines the optimal conditions for dissociation of pathogenic microorganisms according to the type of the sample input to the input unit 210. It is transmitted to the control unit 230. Accordingly, the control unit 230 may control at least one of the rotation time of the driving source, the rotation speed of the driving source, the light amount of the light source unit 250, the wavelength, the light irradiation time, and the type and amount of the emulsifier to be input.

In addition, the sterilization system of pathogenic microorganisms may further include a temperature control unit (not shown). The temperature control unit controls the temperature of the aqueous solution accommodated in the tray (140 in FIG. 1). At this time, the storage unit 220 may be stored in advance the optimized temperature conditions for the desorption of pathogenic microorganisms according to the information of the sample input to the input unit 210. Accordingly, the control unit 230 may control the aqueous solution accommodated in the tray (140 in FIG. 1) to an optimized temperature according to the sample information input to the input unit 210.

In addition, the sterilization system of pathogenic microorganisms may further include a detection unit. The detection unit detects a pathogenic microorganism detached from a sample, and for example, can detect at least one of the type and amount of the pathogenic microorganism. At this time, the storage unit 220 may store the type and risk rating of the pathogenic microorganisms, and the display unit may display at least one of the type, risk rating, and content of the pathogenic microorganism. On the other hand, when the content of the pathogenic microorganisms detected by the detection unit is less than a preset value, the control unit 230 determines that the pathogenic microorganisms are sufficiently sterilized by the light source unit 250, and the driving unit 240, the light source unit 250, and the emulsifier By stopping the operation of the storage portion, unnecessary energy consumption can be prevented.

In addition, the storage unit 220 is pre-stored the conditions for dissociation of pathogenic microorganisms optimized according to at least one of types and amounts of pathogenic microorganisms detected by the detection unit. The control unit 230, according to the information of at least one of the type and amount of pathogenic microorganisms detected by the detection unit, the rotation time of the driving source, the rotation speed of the driving source, the amount of light, wavelength, light irradiation time of the light source unit 250, to be input At least one of the type and amount of the emulsifier can be controlled under optimized conditions.

Hereinafter, the present invention will be described in detail through examples. However, the following examples are only examples for explaining the present invention in more detail and do not limit the scope of the present invention.

Preparation Example 1

Apple samples and paprika samples were inoculated with food poisoning Salmonella Typhimurium , Escherichia coli O157:H7 , Listeria monocytogenes . The specific method is as follows.

Salmonella Typhimurium , E. coli O157:H7 ( Escherichia coli O157:H7 ), Listeria monocytogenes were incubated in tryptic soy broth for 24 hours and centrifuged pellets The solution was resuspended in 0.2% peptone water, and the solution was spot inoculated and dried by dividing it into 10 sections with a volume of 0.1 ml on the surface of each sample cut into 2 cm x 5 cm.

Preparation Example 2

A pathogenic microorganism sterilizing apparatus according to an embodiment of the present invention was prepared. At this time, a tray of 13 x 13 x 13 cm ³ was filled with an aqueous solution so that all the samples were submerged.

As a light source, a krypton-chloride excimer lamp (222 nm wavelength emission) containing no mercury was used, and 110 W power was supplied.

Example 1

An apple sample inoculated with microorganisms according to Preparation Example 1 was put into a tray of a pathogenic microorganism sterilization apparatus according to Preparation Example 2.

Thereafter, the power source (motor) of the pathogenic microbial sterilization apparatus was rotated at 7000 x g to rotate the tray while rotating and simultaneously irradiating with ultraviolet rays for 7 minutes.

After treatment, the samples were stommed in a stomaching bag containing 0.2% peptone water, respectively, and selected mediums for classification, xylose lysine desoxycholate agar (XLD), sorbitol MacConkey agar (SMAC), and oxford agar base (OAB). ). At this time, water used for treatment was also plated on each selective separation medium. Then, the amount of pathogenic microorganisms present in the sample and water was measured.

Example 2

Paprika samples inoculated with microorganisms according to Preparation Example 1 were put into a tray of a pathogenic microorganism sterilization apparatus according to Preparation Example 2.

Thereafter, the power source (motor) of the pathogenic microbial sterilization apparatus was rotated at 7000 x g to rotate the tray while rotating and simultaneously irradiating with ultraviolet rays for 7 minutes.

After treatment, the samples were stommed in a stomaching bag containing 0.2% peptone water, respectively, and selected mediums for classification, xylose lysine desoxycholate agar (XLD), sorbitol MacConkey agar (SMAC), and oxford agar base (OAB). ). At this time, water used for treatment was also plated on each selective separation medium. Then, the amount of pathogenic microorganisms present in the sample and water was measured.

Example 3

An apple sample inoculated with microorganisms according to Preparation Example 1 was put into a tray of a pathogenic microorganism sterilization apparatus according to Preparation Example 2.

As an emulsifier, polysorbate 20 (TWEEN 20), a GRAS (Generally Recognized As Safe) material, was added to the tray. The emulsifier was 0.1 parts by weight per 100 parts by weight of the aqueous solution filled in the tray.

Thereafter, the power source (motor) of the pathogenic microbial sterilization apparatus was rotated at 7000 x g to rotate the tray while rotating and simultaneously irradiating with ultraviolet rays for 7 minutes.

After treatment, the samples were stommed in a stomaching bag containing 0.2% peptone water, respectively, and selected mediums for classification, xylose lysine desoxycholate agar (XLD), sorbitol MacConkey agar (SMAC), and oxford agar base (OAB). ). At this time, water used for treatment was also plated on each selective separation medium. Then, the amount of pathogenic microorganisms present in the sample and water was measured.

Example 4

Paprika samples inoculated with microorganisms according to Preparation Example 1 were put into a tray of a pathogenic microorganism sterilization apparatus according to Preparation Example 2.

As an emulsifier, polysorbate 20 (TWEEN 20), a GRAS (Generally Recognized As Safe) material, was added to the tray. The emulsifier was 0.1 parts by weight per 100 parts by weight of the aqueous solution filled in the tray.

Thereafter, the power source (motor) of the pathogenic microbial sterilization apparatus was rotated at 7000 x g to rotate the tray while rotating and simultaneously irradiating with ultraviolet rays for 7 minutes.

After treatment, the samples were stommed in a stomaching bag containing 0.2% peptone water, respectively, and selected mediums for classification, xylose lysine desoxycholate agar (XLD), sorbitol MacConkey agar (SMAC), and oxford agar base (OAB). ). At this time, water used for treatment was also plated on each selective separation medium. Then, the amount of pathogenic microorganisms present in the sample and water was measured.

Experimental Example 1

In Example 1, the amount of microorganisms detected in the sample and water for each time according to the treatment of the microorganism sterilization apparatus was measured, and the results are shown in FIG. 7.

Referring to FIG. 7, when using the pathogenic microorganism sterilizing apparatus according to an embodiment of the present invention, Salmonella typhimurium and E. coli O157:H7 on the surface of an apple sample were reduced to a detection limit after 5 minutes of treatment (6.12, respectively) Reduction of more than 6.00 units; more than about 99.9999%), Listeria monocytogenes was reduced by 4.26 units (about 99.99%) after 7 minutes of treatment.

That is, when using the pathogenic microorganism sterilizing apparatus according to an embodiment of the present invention, it was confirmed that the pathogenic microorganisms cultured on the surface of the fruits and vegetables within 10 minutes are reduced to 99.99% or more.

Experimental Example 2

In Example 2, after initiating ultraviolet irradiation, the amount of microorganisms detected in the sample and water for each time according to the treatment of the microbial sterilizer was measured, and the results are shown in FIG. 8.

Referring to Figure 8, when using the pathogenic microorganism sterilization apparatus according to an embodiment of the present invention, Salmonella typhimurium and E. coli O157:H7 on the paprika sample surface was reduced to the detection limit after 2 minutes treatment (6.13, respectively) 6.12 logarithmic reduction or lower; about 99.9999% or higher), Listeria monocytogenes decreased by 5.48 (about 99.999%) after 7 minutes.

That is, when using the pathogenic microorganism sterilizing apparatus according to an embodiment of the present invention, it was confirmed that the pathogenic microorganisms cultured on the surface of the fruits and vegetables within 10 minutes are reduced to 99.99% or more.

Experimental Example 3

In Example 3, the amount of microorganisms detected in the sample and water for each time according to the treatment of the microorganism sterilization apparatus was measured, and the results are shown in FIG. 9.

Referring to FIG. 9, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilizing apparatus, E. coli O157:H7 and Salmonella typhimurium on the surface of an apple sample were reduced to a detection limit after 2 minutes treatment ( Reduction of 6.16 and 6.05 logarithms, respectively, about 99.9999%), and Listeria monocytogenes were reduced to detection limits after 7 minutes of treatment (6.15 logarithm reduction; about 99.9999%).

That is, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, it was confirmed that the pathogenic microorganisms cultured on the surface of fruit and vegetables are reduced much faster than when the emulsifier is not used.

Experimental Example 4

In Example 4, the amount of microorganisms detected in the sample and water for each time according to the treatment of the microbial sterilizer was measured, and the results are shown in FIG. 10.

Referring to FIG. 10, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilizing apparatus, E. coli O157:H7 and Salmonella typhimurium on a paprika sample surface were reduced to a detection limit after 1 minute treatment ( Respectively 6.05, 5.95 logarithmic reduction; about 99.9999%), Listeria monocytogenes was reduced to the detection limit after 7 minutes treatment (6.11 logarithmic reduction; about 99.9999%).

That is, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, it was confirmed that the pathogenic microorganisms cultured on the surface of fruit and vegetables are reduced much faster than when the emulsifier is not used.

As described above, the detailed description of the present invention has been made by the embodiments with reference to the accompanying drawings, but the above-described embodiments are only described as preferred examples of the present invention, and the present invention is limited to the above embodiments only. It should not be understood, and the scope of the present invention should be understood in terms of the claims and their equivalent concepts described below.

For example, the drawings are schematically illustrated with each component as a subject for ease of understanding, and the thickness, length, number, etc. of each illustrated component may differ from the actual progress of drawing. In addition, the material, shape, dimension, etc. of each component shown in the above-described embodiments are not limited to one example, and various changes can be made without departing substantially from the effects of the present invention.

110: body
111: engaging projection
111a: Departure prevention member
113: Drive shaft acceptor
120: driving unit
121: drive shaft
130: drive conversion unit
131: Electric shaft
131a: Machinist
133: Conversion axis
133a: Livestock worker
135: support plate
137: Link
140: tray
141: tray body
143: bulkhead
144: joining groove
145: partition
147: ring coupling projection
147a: Deviation prevention protrusion
151: support frame
160: light source unit
161: light source
170: emulsifier storage unit
180: detection unit
210: input
220: storage
230: control
240: driving unit
250: light source unit

Claims (8)

Body;
A tray installed on the body and loaded with an aqueous solution and a sample therein;
A driving unit installed in the body and giving rotational vibration to the tray; And
A light source unit for irradiating sterilized light to the sample loaded on the tray;
Including,
A pathogenic microbial sterilization apparatus for killing pathogenic microorganisms detached by rotational vibration of the sample by a sterilizing light.
The method according to claim 1,
Further comprising an emulsifier storage unit for supplying an emulsifier to the tray on which the sample is loaded, a pathogenic microorganism sterilizing device.
The method according to claim 1,
Further comprising a detection unit for detecting the pathogenic microorganisms detached from the sample, pathogenic microorganisms sterilization apparatus.
The method according to claim 1,
The drive unit comprises a drive conversion unit for converting the rotational motion of the drive source to the rotational vibrational motion of the tray, sterilization device of pathogenic microorganisms.
In the sterilization system of pathogenic microorganisms using the sterilization device of the pathogenic microorganisms according to claim 1,
An input unit for inputting information of a sample loaded in the tray;
A storage unit storing optimized conditions for dissociation of pathogenic microorganisms according to information of a sample input to the input unit; And
A control unit for controlling the operation of the driving unit and the light source unit according to the information of the sample input to the input unit;
Including,
The control unit controls at least one of the rotational speed of the driving source included in the driving unit, the light amount of the light source unit, the light irradiation time and wavelength, the sterilization system of pathogenic microorganisms.
The method according to claim 5,
Further comprising an emulsifier storage unit for supplying an emulsifier to the sample loaded on the tray,
The control unit controls at least one of the type and amount of the emulsifier inputted to the tray according to the information of the input sample, the sterilization system of pathogenic microorganisms.
The method according to claim 5,
Further comprising a temperature control unit for controlling the temperature inside the tray,
The control unit controls the temperature inside the tray according to the information of the input sample, the pathogen microbial sterilization system.
The method according to claim 6,
Further comprising a detection unit for detecting pathogenic microorganisms detached from the sample,
The control unit stops the operation of the driving unit, the light source unit and the emulsifier storage unit when the content of the pathogenic microorganisms detected by the detection unit is less than a preset value, the system for sterilizing pathogenic microorganisms.

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