KR102204874B1 - An apparatus for sterilizing pathogenic microorganism and a system using thereof - Google Patents

Abstract

A device for sterilizing pathogenic microorganisms is provided. In one embodiment, the body; A tray installed in the body and loaded with an aqueous solution and a sample therein; A driving unit installed on the body and providing a rotational vibration motion to the tray; And a light source unit for irradiating sterilizing light to the sample loaded on the tray, and may kill pathogenic microorganisms desorbed by the rotational vibration of the sample by the sterilizing light.

Description

Pathogenic microorganism sterilization device and system using the same {AN APPARATUS FOR STERILIZING PATHOGENIC MICROORGANISM AND A SYSTEM USING THEREOF}

The present invention relates to an apparatus for sterilizing pathogenic microorganisms and a system using the same.

Food poisoning accidents caused by food pathogenic microorganisms are steadily increasing, and these pathogenic microorganisms are known to cause food poisoning through cross-contamination of foods while being present on various surfaces in the food itself or in the cooking and storage environment of food. In addition, there are many materials such as stainless steel, plastic, and rubber that are easy to form a biofilm by microorganisms settling in the food contact surface, and thus biofilm-related infectious diseases also occur.

Meanwhile, high pressure treatment, electric fields treatment, ultrasonic treatment, gaseous sanitizer treatment, acid/base wash solution to control pathogenic microorganisms existing on the surface of such foods and appliances to date. (acid/alkali wash) treatment, chlorine treatment, and peracid sanitizer treatment have been studied.

However, in the case of electric field, ultrasonic treatment, etc., there is a disadvantage in that the area available for sterilization treatment is narrow in practical application, and only the use of fixed equipment is possible, and installation and treatment costs are high. In addition, in the case of gas sterilizers, safety problems for workers may arise.

In the case of various chemical sterilization and disinfectants, residues may be generated after treatment, and harmful by-products may be generated by reacting with organic substances, and corrosion may be caused depending on the type of contact surface. Due to this problem, a washing process that requires a large amount of washing water after treatment with a chemical disinfectant disinfectant is required, and in this process, a problem of re-contamination of pathogenic microorganisms due to reuse of washing water may occur.

Accordingly, there has been a need to develop a sterilizer that can be easily used in food manufacturing processes and catering establishments and has an excellent effect of removing pathogenic microorganisms, and ultraviolet (ultraviolet) treatment with low treatment cost and no harmful by-products is in the spotlight.

However, in spite of the many advantages of conventional ultraviolet treatment, there is a disadvantage in that the penetration depth of ultraviolet rays is low, so that pathogenic microorganisms existing in the gaps of food and appliances cannot be sufficiently removed.

Therefore, there is a need to develop a sterilization device and a sterilization system capable of efficiently sterilizing pathogenic microorganisms using an ultraviolet treatment method.

The problem to be solved by the present invention is to provide an apparatus for sterilizing pathogenic microorganisms and a system using the same, which can greatly increase the sterilization effect while sterilizing treatment is possible at low cost using ultraviolet rays.

In order to solve the above problems, the present invention rotates and vibrates so as not to damage the sample, so that pathogenic microorganisms that propagate between the gaps on the sample surface are separated from the sample surface without damaging the sample, and the separated pathogenic microorganisms are harmful by-products. It is intended to provide a pathogenic microorganism sterilization device that can efficiently sterilize without the occurrence of

The present invention effectively desorbs pathogenic microorganisms from the surface of a sample without damaging the sample, and can efficiently sterilize the desorbed pathogenic microorganisms without generating harmful by-products.

1 shows an apparatus for sterilizing pathogenic microorganisms according to an embodiment of the present invention.
2 shows a driving unit according to an embodiment of the present invention.
3 shows a tray according to an embodiment of the present invention.
Figure 4 shows a microbial sterilization apparatus according to another embodiment of the present invention.
Figure 5 shows a microbial sterilization apparatus according to another embodiment of the present invention.
6 is a block diagram schematically showing a system for sterilizing pathogenic microorganisms according to an embodiment of the present invention.
7 to 10 show the detection results of pathogenic microorganisms according to Experimental Examples 1 to 4 of the present invention, respectively.

Hereinafter, embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only this embodiment makes the disclosure of the present invention complete, and the contents of the present invention are provided to those of ordinary skill in the art. It is provided to inform you more completely.

In this specification, when an element is referred to as being positioned'above' or'below' another element, it means that the element is positioned directly above or'below' another element, or an additional element is placed between those elements. It includes all meanings that can be intervened. In this specification, the term'upper' or'lower' is a relative concept set at the observer's point of view, and if the observer's point of view is different,'upper' may mean'lower', and'lower' means'upper'. It could mean.

The same reference numerals in the plurality of drawings refer to substantially the same elements. In addition, terms such as'include' or'have' are intended to designate the existence of a feature, number, step, action, component, part, or combination thereof to be described, and one or more other features, numbers, or steps. It is to be understood that it does not preclude the possibility of addition or presence of, operations, components, parts, or combinations thereof.

An embodiment of the present invention, the body; A tray installed in the body and loaded with an aqueous solution and a sample therein; A driving unit installed on the body and providing a rotational vibration motion to the tray; And a light source unit for irradiating sterilizing light to the sample loaded on the tray, and killing pathogenic microorganisms desorbed by the sterilizing light by the rotational vibration of the sample.

For example, it may further include an emulsifier storage unit for supplying an emulsifier to the tray in which the sample is loaded.

In addition, it may further include a detection unit for detecting the pathogenic microorganism to be released from the sample.

Meanwhile, the driving unit may include a driving conversion unit that converts a rotational motion of a driving source into a rotational vibrational motion of the tray.

Another embodiment of the present invention is a system for sterilizing pathogenic microorganisms using the aforementioned device for sterilizing pathogenic microorganisms, comprising: an input unit for inputting information on a sample loaded on the tray; A storage unit for storing a desorption condition of pathogenic microorganisms optimized according to information of a sample input to the input unit; And a control unit for controlling the operation of the driving unit and the light source unit according to the sample information input to the input unit, wherein the control unit includes a rotational speed of the driving source included in the driving unit, an amount of light of the light source unit, a light irradiation time and wavelength. It provides a system for sterilizing pathogenic microorganisms, controlling at least any one of them.

At this time, it further includes an emulsifier storage unit for supplying an emulsifier to the sample loaded on the tray, and the control unit may control at least one of the type and amount of the emulsifier injected into the tray according to the input sample information. .

For example, a temperature control unit for controlling a temperature inside the tray may be further included, and the control unit may control the temperature inside the tray according to the input sample information.

On the other hand, it further comprises a detection unit for detecting the pathogenic microorganisms desorbed from the sample, the control unit to stop the operation of the driving unit, the light source unit and the emulsifier storage unit when the content of the pathogenic microorganism detected by the detection unit is less than a preset value. I can.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings.

1 shows an apparatus for sterilizing pathogenic microorganisms according to an embodiment of the present invention.

Referring to FIG. 1, an apparatus for sterilizing pathogenic microorganisms includes a body 110, a tray 140, a driving unit 120, and a light source unit 160.

The body 110 has a predetermined volume and supports the tray 140 to rotate and vibrate. The body 110 accommodates a driving source (not shown) such as a motor therein, and a power supply line for supplying power to the driving unit 120 and the light source unit 160 is accommodated.

The tray 140 is to be loaded with an aqueous solution and a sample therein, and is installed on the body 110. The tray 140 rotates and vibrates by the drive unit 120 to remove pathogenic microorganisms present on the sample surface from the sample without damaging the sample. For example, since the inside of the tray 140 is filled with an aqueous solution, damage to the sample can be prevented when the tray 140 rotates and vibrates.

The tray 140 may be installed on the body 110 as shown in FIG. 1. As another example, the tray 140 is installed inside the body 110 so that a body cover (not shown) covers the body 110 so that the tray 140 and the body 110 can be moved integrally, so that it is portable. An easy form may be applied.

The tray 140 accommodates aqueous solutions and samples therein, is loaded on the support plate 135 and receives rotational force and centrifugal force through the support plate 135, and the position is supported by the connecting ring 137 to rotate and vibrate in place. do. As a result, the sample contained therein is not destroyed and pathogenic microorganisms can be separated from the sample surface.

The tray 140 may have one receiving space formed therein, and a plurality of trays 140 may be formed. The tray 140 having the plurality of accommodation spaces will be described later with reference to FIG. 3.

A drive shaft receiving hole 113 is formed through the upper surface of the body 110 to expose the drive shaft 121 of the drive source accommodated therein to be coupled to the drive conversion unit 130 to the outside. The drive shaft 121 protrudes upward of the body 110 through the drive shaft receiving hole 113, and each component of the drive conversion unit 130 is coupled to the protruding drive shaft 121.

A coupling protrusion 111 to which the connecting ring 137 is coupled is provided in the four corner regions of the upper side of the body 110. The coupling protrusion 111 is provided so as to protrude from the upper plate surface of the body 110 to a predetermined height, and one end of the connection ring 137 is coupled. The coupling protrusion 111 is coupled to one end of the connection ring 137 and fixes the position so that the tray 140 can be rotated and vibrated.

The connecting ring 137 interconnects the tray 140 and the body 110 so that the tray 140 vibrates left and right in place and rotates without being idle together with the support plate 135. One end of the connecting ring 137 is coupled to the engaging protrusion 111 of the body 110, and the other end is coupled to the annular engaging protrusion 147 of the tray 140. The connecting ring 137 is provided as a member having an elastic force so that the distance between the tray 140 and the body 110 is elastically adjusted so that the tray 140 can be vibrated left and right.

At the upper end of the coupling protrusion 111, a detachment preventing protrusion 111a that holds the position of the coupling ring 137 is coupled so that the coupled connection ring 137 is not separated from the outside by vibration. The separation prevention protrusion 111a is formed to extend a certain length outward in the radial direction of the coupling protrusion 111 so that when the connecting ring 137 is about to be separated in the upward direction due to an external impact, the connecting ring 137 is contacted and supported and separated. Prevent. A rubber cover (not shown) may be coupled to the separation prevention protrusion 111a to prevent slipping of the connection ring 137.

The drive conversion unit 130 is connected to the drive shaft 121 to convert the rotational motion of the drive source into the rotational vibrational motion of the tray 140, so that pathogenic microorganisms are not damaged from the sample loaded on the tray 140. Let's get rid of it.

The light source unit 160 irradiates a sterilizing light source and, for example, may irradiate ultraviolet rays, and, for example, may irradiate a UV-C wavelength of 200 to 280 nm. Since the UV-C wavelength has high energy, it has high sterilization efficiency by destroying DNA of microorganisms. That is, the light source unit 160 may sterilize pathogenic microorganisms desorbed from the sample surface as a result of the rotational vibration of the tray 140 with a sterilizing light source. In addition, the light source 161 installed in the light source unit 160 is not particularly limited as long as it emits a sterilizing light source such as an LED or an excimer lamp.

As shown in FIG. 1, the light source unit 160 may be installed to irradiate the sterilizing light source from the top to the bottom of the tray 140. Meanwhile, when the tray 140 is installed inside the body 110, the light source unit 160 may be installed on the body cover or installed on the side of the tray 140 inside the body 110.

The light source unit 160 includes a support frame 151 vertically coupled to the body 110. The support frame 151 adjusts the height at which the light source 161 is supported. The support frame 151 may be provided so that the height is adjustable.

Meanwhile, an emulsifier (not shown) may be supplied to the tray 140 shown in FIG. 1. The emulsifier may be added while the tray 140 is rotating or vibrated, or may be pre-injected prior to the rotary vibration. The emulsifier may include both a hydrophilic group and a hydrophobic group in the molecule, thereby reducing the interfacial tension between the liquid and the solid.

Since the surface of a sample, for example, a fruit or vegetable, has hydrophobicity and the surface of pathogenic microorganisms is also hydrophobic, it is not easy to remove pathogenic microorganisms from the surface of the sample supported in the aqueous solution. Accordingly, in the present invention, an emulsifier having a hydrophilic group and a hydrophobic group in the molecule is applied to a pathogenic microorganism sterilization apparatus, so that the pathogenic microorganism can be effectively desorbed from the sample surface.

The type of emulsifier is an emulsifier having a hydrophilic group and a hydrophobic group in the molecule, for example, polysorbate, acrylate copolymer, trimethylpentanediol/adipic acid/glycerin crosspolymer, trimethylpentanediol/adipic acid copolymer, ethyl It may include at least one of hexyl stearate, ethylhexyl salicylate, alkyl methacrylate copolymer, octocrylene, ethylene oxide/propylene oxide block copolymer, and polyquaternium-101.

For example, the emulsifier is a GRAS (generally recognized as safe) material, and includes a polysorbate compound, and includes, but is not limited to, polysorbate 20, 60, and 80, for example.

The emulsifier may be applied in an amount of 0.1 parts by weight or less per 100 parts by weight of the aqueous solution to be filled in the tray 140, for example, 0.001 parts by weight to 0.1 parts by weight.

In addition, the emulsifier may use sodium lauryl sulfate, which is a GRAS emulsifier, alone or together with the polysorbate compound, and in this case, the sodium lauryl sulfate may be contained in an amount of 25 ppm to 1000 ppm.

2 shows a driving unit according to an embodiment of the present invention.

Referring to FIG. 2, the driving unit 120 includes a driving conversion unit 130.

The drive conversion unit 130 receives the driving force of the driving source and converts the rotational motion of the driving source into a rotational vibrational motion.

The drive conversion unit 130 is disposed in an upper region of the transmission shaft 131 and the transmission shaft 131 coupled to coincide with the axis of the drive shaft 121 and is arranged eccentrically with a predetermined length with respect to the axis of the drive shaft 121 It includes a conversion shaft 133 and a support plate 135 disposed in the upper region of the conversion shaft 133 and supporting the tray 140. The drive conversion unit 130 interlocks with the connection ring 137 connecting the coupling protrusion 111 and the tray 140 to transmit the rotational motion of the driving source to the tray 140 as a rotational vibrational motion.

The transmission shaft 131 is coupled on the coaxial of the drive shaft 121 and rotates in conjunction with the drive of the drive shaft 121. The transmission shaft 131 accommodates the drive shaft 121 protruding to the outside of the body 110 in the shaft receiving hole 131a. When power is applied to the transmission shaft 131 and the driving shaft 121 is driven, the transmission shaft 131 rotates together and transmits the rotational force to the tray 140.

The conversion shaft 133 is disposed between the transmission shaft 131 and the support plate 135 and is coupled with the drive shaft 121 so as to be eccentric to a predetermined length with respect to the axial direction of the drive shaft 121. Thereby, the conversion shaft 133 rotates eccentrically when the drive shaft 121 rotates so that a greater centrifugal force acts on the tray 140 compared to when it rotates in the positive axis direction. Centrifugal force acting on the tray 140 acts on the sample so that pathogenic microorganisms can be separated into a diluent.

The support plate 135 supports the tray 140 so that rotational force and centrifugal force are transmitted to the tray 140 through the transmission shaft 131 and the conversion shaft 133. The support plate 135 is rotatably coupled to the drive shaft 121 and fixedly coupled to the tray 140. Here, when the tray 140 is loaded on the support plate 135, a fixing member (not shown) for fixing the combined state of the two may be used.

3 shows a tray according to an embodiment of the present invention.

The tray 140 may be formed to have a plurality of accommodation spaces. The tray 140 is a box-shaped tray body 141 with an open upper area, a partition wall 143 that divides the tray body 141 into a plurality of accommodation spaces, and is detachably coupled between the partition wall 143 It may include a partition (145).

The tray body 141 is made of a plastic material and is provided to have durability that is not damaged even in rotational vibration. For example, a transparent member such as PET may be applied. The partition wall 143 divides the inner space of the tray main body 141 into a plurality of spaces. The partition wall 143 may be provided in a cross shape so that the inner space is divided into a receiving space capable of accommodating four samples, or may be provided in a straight shape capable of accommodating two samples.

In addition, the partition wall 143 and the upper side of the tray body 141 may be provided with a plurality of coupling grooves 144 to which the partitions 145 can be detachably coupled at regular intervals. Accordingly, the operator can actively adjust the size of the receiving space by adjusting the coupling position of the partition 145 according to the size of the sample. That is, a plurality of trays 140 are provided so that the receiving spaces are provided differently, and may be used by being replaced with the support plate 135 according to the number of samples.

The lower region of the tray main body 141 is provided with a ring coupling protrusion 147 to which the connection ring 137 is coupled. The ring coupling protrusion 147 is coupled with the connecting ring 137 so that the tray 140 vibrates left and right without rotating together with the support plate 135 and rotates in place. The ring coupling protrusion 147 is formed to extend a predetermined length to the lower region of the tray main body 141 so that the connection ring 137 is coupled. The lower end of the ring coupling protrusion 147 is provided with a separation preventing protrusion 147a that prevents the separation of the connecting ring 137.

Figure 4 shows a microbial sterilization apparatus according to another embodiment of the present invention.

4, the microbial sterilization device further includes an emulsifier storage unit 170.

The emulsifier storage unit 170 supplies an emulsifier to the tray 140 in which the sample is loaded. For example, the emulsifier storage unit 170 may supply different amounts or types of emulsifiers depending on the sample, and the amount or type may be controlled by a controller to be described later. The type of the emulsifier may be applied as described above with reference to FIG. 1. In addition, the emulsifier storage unit 170 may be classified and stored for each type of emulsifier, as in the tray 140 described above with reference to FIG. 3, May be put into the tray 140.

Figure 5 shows a microbial sterilization apparatus according to another embodiment of the present invention.

Referring to FIG. 5, the microbial sterilization apparatus further includes a detection unit 180 for detecting pathogenic microbes that are desorbed from the sample.

The detection unit 180 may detect, for example, at least one of the type and amount of pathogenic microorganisms.

Meanwhile, the apparatus for sterilizing microorganisms described above with reference to FIGS. 1 to 5 may further include a display unit (not shown). The display unit may display the operating state of the microbial sterilization device to the user. For example, the display unit is the driving time of the microbial sterilization device, the rotational speed of the driving source, the amount of light of the light source unit 160, the wavelength, the light irradiation time, the type and amount of the emulsifier injected, and the pathogenic microorganism detected by the detection unit 180. At least one of the type and amount of can be displayed.

In addition, the display unit may include an operation panel (not shown). The operation panel is capable of driving the sterilization device for pathogenic microorganisms by the user, and may include, for example, a power button, a timer, and a driving control button. The driving control button may control at least one of a rotation time of the driving source, a rotation speed of the driving source, an amount of light of the light source unit 160, a wavelength, a light irradiation time, and a type and amount of an emulsifier to be introduced.

6 is a schematic block diagram showing a system for sterilizing pathogenic microorganisms according to an embodiment of the present invention.

Referring to FIG. 6, the system for sterilizing pathogenic microorganisms includes an input unit 210, a storage unit 220, a control unit 230, a driving unit 240, and a light source unit 250.

The driving unit 240 and the light source unit 250 may apply the above description with reference to FIGS. 1 to 5. In addition, a configuration not shown in FIG. 6 will be described below with reference to FIGS. 1 to 5.

The input unit 210 inputs information on a sample loaded on a tray (140 in FIG. 1). For example, information on at least one of the type, weight, and volume of the sample may be input.

The storage unit 220 stores in advance conditions for desorption of pathogenic microorganisms optimized according to the information of the sample input to the input unit 210. For example, desorption conditions suitable for the type, weight, and volume conditions of the sample are stored. The desorption condition may be at least one of a rotation time of the driving source, a rotation speed of the driving source, an amount of light of the light source unit 160, a wavelength, a light irradiation time, and a type and amount of an emulsifier to be introduced. For example, the removal condition may be displayed on the display unit.

The control unit 230 controls at least one of the driving unit 240, the light source unit 250, and the emulsifier storage unit (170 in FIG. 4) according to the sample information input to the input unit 210. For example, the information of the sample input to the input unit 210 is transferred to the storage unit 220, and the storage unit 220 determines the conditions for desorption of pathogenic microorganisms optimized according to the type of sample input to the input unit 210. It is transmitted to the control unit 230. Accordingly, the control unit 230 may control at least one of the rotation time of the driving source, the rotation speed of the driving source, the amount of light of the light source unit 250, the wavelength, the light irradiation time, and the type and amount of the emulsifier to be introduced.

In addition, the system for sterilizing pathogenic microorganisms may further include a temperature control unit (not shown). The temperature control unit controls the temperature of the aqueous solution contained in the tray (140 in FIG. 1). In this case, the storage unit 220 may pre-store an optimized temperature condition for desorption of pathogenic microorganisms according to information of a sample input to the input unit 210. Accordingly, the control unit 230 may control the aqueous solution accommodated in the tray (140 in FIG. 1) to an optimized temperature according to the information of the sample input to the input unit 210.

In addition, the system for sterilizing pathogenic microorganisms may further include a detection unit. The detection unit detects pathogenic microorganisms that are desorbed from the sample, and may detect at least one of the types and amounts of pathogenic microorganisms, for example. In this case, the storage unit 220 may store the type and risk level of the pathogenic microorganism, and the display unit may display at least one of a type, a risk level, and a content of the pathogenic microorganism. On the other hand, when the content of the pathogenic microorganism detected by the detection unit is less than a preset value, the controller 230 determines that the pathogenic microorganism has been sufficiently sterilized by the light source unit 250, and the driving unit 240, the light source unit 250 and the emulsifier By stopping the operation of the storage unit, unnecessary energy consumption can be prevented.

In addition, the storage unit 220 stores in advance conditions for desorption of pathogenic microorganisms optimized according to information of at least one of the types and amounts of pathogenic microorganisms detected by the detection unit. According to at least one of the types and amounts of pathogenic microorganisms detected by the detection unit, the control unit 230 may perform the rotation time of the driving source, the rotation speed of the driving source, the amount of light of the light source unit 250, the wavelength, the light irradiation time, and At least one of the type and amount of the emulsifier can be controlled under optimized conditions.

Hereinafter, the present invention will be described in detail through examples. However, the following examples are only examples for explaining the present invention in more detail, and do not limit the scope of the present invention.

Preparation Example 1

Food poisoning bacteria Salmonella Typhimurium , E. coli O157:H7 ( Escherichia coli O157:H7 ), Listeria monocytogenes were inoculated into apple samples and paprika samples. The specific method is as follows.

Salmonella Typhimurium , E. coli O157:H7 ( Escherichia coli O157:H7 ), Listeria monocytogenes were incubated in tryptic soy broth for 24 hours and then centrifuged. It was resuspended in 0.2% peptone water, and the solution was spot inoculated and dried on the surface of each sample cut into 2cm x 5cm by dividing into 10 zones with a volume of 0.1ml.

Preparation Example 2

An apparatus for sterilizing pathogenic microorganisms according to an embodiment of the present invention was prepared. At this time, a 13 x 13 x 13 cm ³ volume tray was filled with an aqueous solution sufficient to submerge all samples.

As a light source, a krypton-chloride excimer lamp (222 nm wavelength emission) containing no mercury was used, and 110 W power was supplied.

Example 1

The apple sample inoculated with microorganisms according to Preparation Example 1 was put into the tray of the pathogenic microorganism sterilization apparatus according to Preparation Example 2.

Thereafter, the power source (motor) of the pathogenic microorganism sterilization device was rotated at 7000 x g, and the tray was rotated and vibrated while UV irradiation was performed for 7 minutes.

After treatment, the sample is stomached in a stomaching bag containing 0.2% peptone water, and each selective fractionation medium is XLD (xylose lysine desoxycholate agar), SMAC (sorbitol MacConkey agar), OAB (oxford agar base). ) Was smeared. At this time, water used for treatment was also dispensed and smeared in each selective fractionation medium. Thereafter, the population of pathogenic microorganisms present in the sample and water was measured.

Example 2

The paprika sample inoculated with microorganisms according to Preparation Example 1 was added to the tray of the pathogenic microorganism sterilization apparatus according to Preparation Example 2.

Thereafter, the power source (motor) of the pathogenic microorganism sterilization device was rotated at 7000 x g, and the tray was rotated and vibrated while UV irradiation was performed for 7 minutes.

After treatment, the sample is stomached in a stomaching bag containing 0.2% peptone water, and each selective fractionation medium is XLD (xylose lysine desoxycholate agar), SMAC (sorbitol MacConkey agar), OAB (oxford agar base). ) Was smeared. At this time, water used for treatment was also dispensed and smeared in each selective fractionation medium. Thereafter, the population of pathogenic microorganisms present in the sample and water was measured.

Example 3

The apple sample inoculated with microorganisms according to Preparation Example 1 was put into the tray of the pathogenic microorganism sterilization apparatus according to Preparation Example 2.

As an emulsifier, polysorbate 20 (TWEEN 20), which is a GRAS (Generally Recognized As Safe) material, was added to the tray. The emulsifier was 0.1 parts by weight per 100 parts by weight of the aqueous solution filled in the tray.

Thereafter, the power source (motor) of the pathogenic microorganism sterilization device was rotated at 7000 x g, and the tray was rotated and vibrated while UV irradiation was performed for 7 minutes.

After treatment, the sample is stomached in a stomaching bag containing 0.2% peptone water, and each selective fractionation medium is XLD (xylose lysine desoxycholate agar), SMAC (sorbitol MacConkey agar), OAB (oxford agar base). ) And smeared. At this time, water used for treatment was also dispensed and smeared in each selective fractionation medium. Then, the amount (population) of pathogenic microorganisms present in the sample and water was measured.

Example 4

The paprika sample inoculated with microorganisms according to Preparation Example 1 was added to the tray of the pathogenic microorganism sterilization apparatus according to Preparation Example 2.

Polysorbate 20 (TWEEN 20), a GRAS (Generally Recognized As Safe) material as an emulsifier, was added to the tray. The emulsifier was 0.1 parts by weight per 100 parts by weight of the aqueous solution filled in the tray.

Thereafter, the power source (motor) of the pathogenic microorganism sterilization device was rotated at 7000 x g, and the tray was rotated and vibrated while UV irradiation was performed for 7 minutes.

After treatment, the sample is stomached in a stomaching bag containing 0.2% peptone water, and each selective fractionation medium is XLD (xylose lysine desoxycholate agar), SMAC (sorbitol MacConkey agar), OAB (oxford agar base). ) Was smeared. At this time, water used for treatment was also dispensed and smeared in each selective fractionation medium. Thereafter, the population of pathogenic microorganisms present in the sample and water was measured.

Experimental Example 1

In Example 1, the amount of microorganisms detected in the sample and water for each time according to the microbial sterilization device treatment was measured, and the results are shown in FIG. 7.

Referring to FIG. 7, when using the pathogenic microorganism sterilization apparatus according to an embodiment of the present invention, Salmonella typhimurium and E. coli O157:H7 on the surface of an apple sample were reduced to the detection limit after 5 minutes treatment (each 6.12, Reduction of more than 6.00 units; more than about 99.9999%), Listeria monocytogenes decreased by 4.26 units (about 99.99%) after 7 minutes treatment.

That is, in the case of using the pathogenic microorganism sterilization apparatus according to an embodiment of the present invention, it was confirmed that the pathogenic microorganisms cultured on the surface of fruit or vegetables were reduced to 99.99% or more within 10 minutes.

Experimental Example 2

In Example 2, after starting the ultraviolet irradiation, the amount of microorganisms detected in the sample and water for each time according to the treatment of the microbial sterilizer was measured, and the results are shown in FIG. 8.

8, when using the pathogenic microorganism sterilization apparatus according to an embodiment of the present invention, Salmonella typhimurium and E. coli O157:H7 on the paprika sample surface were reduced to the detection limit after 2 minutes treatment (each 6.13, 6.12 logarithm or more reduction; about 99.9999% or more), Listeria monocytogenes decreased by 5.48 (about 99.999%) logarithm after 7 minutes treatment.

That is, in the case of using the pathogenic microorganism sterilization apparatus according to an embodiment of the present invention, it was confirmed that the pathogenic microorganisms cultured on the surface of fruit or vegetables were reduced to 99.99% or more within 10 minutes.

Experimental Example 3

In Example 3, the amount of microorganisms detected in the sample and water for each time according to the microbial sterilization device treatment was measured, and the results are shown in FIG. 9.

Referring to FIG. 9, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, E. coli O157:H7 and Salmonella typhimurium on the surface of an apple sample were reduced to the detection limit after 2 minutes treatment ( 6.16, 6.05 log reduction; about 99.9999%), and Listeria monocytogenes decreased to the detection limit after 7 min treatment (6.15 log reduction; about 99.9999%).

That is, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, it was confirmed that pathogenic microorganisms cultured on the surface of fruits and vegetables were reduced much faster than when the emulsifier was not used.

Experimental Example 4

In Example 4, the amount (population) of microorganisms detected in the sample and water for each time according to the microbial sterilization device treatment was measured, and the results are shown in FIG. 10.

Referring to FIG. 10, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, E. coli O157:H7 and Salmonella typhimurium on the paprika sample surface were reduced to the detection limit after 1 minute treatment ( The number of logarithms decreased by 6.05 and 5.95, respectively; about 99.9999%), and Listeria monocytogenes was reduced to the detection limit after 7 minutes treatment (6.11 log reduction; about 99.9999%).

That is, when the emulsifier according to an embodiment of the present invention is applied to a pathogenic microorganism sterilization device, it was confirmed that pathogenic microorganisms cultured on the surface of fruits and vegetables were reduced much faster than when the emulsifier was not used.

As described above, a detailed description of the present invention has been made by an embodiment with reference to the accompanying drawings, but the above-described embodiment has been described with reference to a preferred example of the present invention, so that the present invention is limited to the above embodiment. It should not be understood, and the scope of the present invention should be understood as the following claims and equivalent concepts.

For example, the drawings schematically show each component as a subject to aid understanding, and the thickness, length, number, etc. of each component shown may be different from the actual one in the progress of drawing creation. In addition, the material, shape, dimensions, etc. of each constituent element shown in the above embodiments are not particularly limited as an example, and various changes can be made without substantially departing from the effects of the present invention.

110: body
111: coupling protrusion
111a: separation prevention member
113: drive shaft receiving hole
120: drive unit
121: drive shaft
130: drive conversion unit
131: transmission shaft
131a: shaft bearing ball
133: conversion axis
133a: shaft bearing ball
135: support plate
137: connection
140: tray
141: tray body
143: bulkhead
144: coupling groove
145: partition
147: ring bonding protrusion
147a: departure prevention protrusion
151: support frame
160: light source unit
161: light source
170: emulsifier storage unit
180: detection unit
210: input unit
220: storage unit
230: control unit
240: drive unit
250: light source unit

Claims (8)

Body;
A tray installed in the body and loaded with an aqueous solution and a sample therein;
A driving unit installed on the body and providing a rotational vibration motion to the tray;
A connecting ring that interconnects the tray and the body and is an elastic member; And
A light source unit for irradiating sterilizing light onto the sample loaded on the tray;
Including,
The drive unit includes a drive conversion unit for converting the rotational motion of the drive source into the rotational vibrational motion of the tray,
The drive conversion unit includes a support plate for supporting the tray,
The tray is supported by the linking ring to rotate and vibrate in place, the linking ring interlocks with the drive conversion unit to transmit the rotational motion of the drive source to the tray as a rotational vibrating motion,
Pathogenic microorganism sterilization apparatus for killing pathogenic microorganisms desorbed by the rotational vibration of the sample by sterilizing light.
The method according to claim 1,
Further comprising an emulsifier storage unit for supplying an emulsifier to the tray in which the sample is loaded, pathogenic microorganism sterilization device.
The method according to claim 1,
A device for sterilizing pathogenic microorganisms, further comprising a detection unit for detecting pathogenic microorganisms that are desorbed from the sample.
The method according to claim 1,
The drive conversion unit,
A transmission shaft coupled to coincide with the axis of the drive shaft;
A conversion shaft disposed in an upper region of the transmission shaft and disposed to be eccentric with a predetermined length with respect to the axis of the drive shaft; And
A support plate disposed in an upper region of the conversion shaft and supporting the tray;
Containing, pathogenic microorganism sterilization device.
In the sterilization system for pathogenic microorganisms using the sterilization device for pathogenic microorganisms according to claim 1,
An input unit for inputting information of a sample loaded on the tray;
A storage unit for storing a desorption condition of pathogenic microorganisms optimized according to information of a sample input to the input unit; And
A control unit for controlling the operation of the driving unit and the light source unit according to the information of the sample inputted to the input unit;
Including,
The control unit controls at least one of a rotation speed of a driving source included in the driving unit, an amount of light of the light source unit, a light irradiation time, and a wavelength.
The method of claim 5,
Further comprising an emulsifier storage unit for supplying an emulsifier to the sample loaded on the tray,
The control unit controls at least one of a type and an amount of an emulsifier injected into the tray according to the input sample information.
The method of claim 5,
Further comprising a temperature control unit for controlling the temperature inside the tray,
The control unit controls the temperature inside the tray according to the input sample information.
The method of claim 6,
Further comprising a detection unit for detecting the pathogenic microorganisms desorbed from the sample,
The control unit stops the operation of the driving unit, the light source unit, and the emulsifier storage unit when the content of the pathogenic microorganism detected by the detection unit is less than a preset value.

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