KR20200083057A - composition for improving skin inflammation - Google Patents

Abstract

The present invention relates to a cosmetic composition having an anti-inflammatory effect, and more specifically, to a cosmetic composition having an anti-inflammatory effect, containing an arctium minus extract, a lichen extract, a white leaf extract and an oat extract as an active ingredient. The cosmetic composition having an anti-inflammatory effect according to the present invention is safe for the skin and relieves skin troubles.

Description

Composition for improving skin inflammation}

The present invention relates to a cosmetic composition having an anti-inflammatory effect, and more particularly, to a cosmetic composition having an anti-inflammatory effect, which can be safely used without side effects on the skin and has a skin trouble-relieving effect.

Cosmetics are products that are used to protect the skin and to beautify and clean the skin, but their composition inevitably includes ingredients different from the purpose of skin protection. These ingredients include surfactants, preservatives, fragrances, sunscreens, and pigments, as well as several other ingredients to impart other efficacy and effectiveness. These ingredients are generally known to cause various problems such as inflammation, rash and edema on the skin. (Maibach. HI, Contact Dermatitis, 6. 369-404, 1980) In addition, sebum, sweat and cosmetic ingredients released from the body It is well known that heavy fatty acids, higher alcohols, protein components, etc. are decomposed into highly toxic substances by dermatophytes on the skin, and skin inflammation may be caused by them. .

Inflammatory reactions are manifested in five phenomena: redness, tingling, burning, swelling, and tissue changes, which are physiological reactions to protect the body from invasion and mechanical damage from harmful surroundings, such as bacteria. This inflammatory phenomenon leads to a large increase in various types of polynuclear leukocytes (PMNs) and immune substances, and these increased cells can be treated and defended by secreting inflammatory cell products, various types of proteases and cytokines. Allows you to However, this action can also cause harmful damage to adjacent tissue cells and non-cellular components. As it is EK, under proper conditions, normal function is restored after the initial state of inflammation, but if irritants that stimulate the inflammation disappear or are continuously made, chronic inflammation occurs as a result, resulting in more serious tissue damage.

Representative skin problems associated with inflammation include atopy and acne, and the description of atopy is as follows.

Atopic dermatitis is characterized by severe itching. When it is irritated to the skin, itching becomes more severe, and when it is scratched more and more, it is wounded, causing bacterial infection (yellow staphylococcus aureus, streptococcus, etc.) and inflammation. One of the important factors among atopy problems is secondary infection and superantigen toxin. 80-90% of patients with atopic skin have infections of Staphylococcus aureus and Streptococcus pyogenes on the skin, where anti-staphilococcus IgE is produced, which is a superantigen that stimulates T-cell responses It is caused and exacerbated by toxins. Staphylococcus aureus superantigen toxin selectively stimulates T lymphocytes through Langerhans intracellular antigen-presenting cells to produce interleukin-1 and TNF-alpha, and various intracellular cytokines in T-cells stimulated by them It secretes a toxin called'to cause inflammation'. In other words, the superantigen toxin promotes the secretion of interleukin-4 and interroutine-5, inhibiting the secretion of IFN-gamma, and increases the number of CLAs, eventually promoting the production of IgE.

Second, patients infected with Staphylococcus aureus secrete a substance called histamine from basophils by an IgE antibody against Staphylococcus aureus, which exacerbates atopic dermatitis.

Therefore, the use of moisturizers is often recommended to prevent skin dryness, and secondary infection and the control of the superantigens resulting therefrom are very important. In other words, atopy sees genetic defects as a basic etiology, but promotes triggering by various external acquired factors.

Atopic induced exacerbations include genetic factors, sensitive and dry skin, metabolic factors, excessive production of IgE, dysregulation of the autonomic nervous system, psychological factors, and irritating factors.

Atopy is a disease whose mechanism is never simple. From an immunological point of view, it is not a single immunological problem, but a problem with various patterns. As an example, the problem of gamma interferon, which suppresses excessive production of IgE, occurs even when the production is less than the average value, but occurs less than the interleukin-4, which has an opposite action. In some cases, even if it is normal in terms of quantity or quality, it may occur when sensitivity to interferon stimulation is low.

The key material of these various inflammatory processes is arachidonic acid, which is converted from cell membrane lipids by an enzyme called Phospholipase A2 (PLA2) activated by stimulation, and arachidonic acid is again cyclooxygenase (cyclooxygenase). , Hereinafter referred to as'') or metabolizes to inflammatory mediators prostaglandins and leulotrienes via two pathways of lipoxygenase (mediated by inflammatory responses) (Thomas, R., Rev. Phsiol.Bioche.Pharmacol., 100, 1984)

Recently, research on a method for reducing the generation of skin irritation and inflammation has been actively conducted. As a result of studies so far, tissues or plasma by nerve peptides or stimuli such as substance P secreted from neurons Various cytokines, prostaglandin E2 (hereinafter referred to as ``hereinafter'') formed by COX pathway, or by kinins such as bradykinin, which are activated in, are involved in edema formation and vasodilation, causing inflammation and pain. It is known. Accordingly, as an anti-inflammatory agent, neuropeptide antagonists including substance P, Bradkinin antagonists, COX inhibitors, etc. have been proposed, but anti-inflammatory agents known to date have most problems in terms of safety for skin and stability when included in products. Therefore, its use is limited.

In order to solve the above problems, the present invention is to provide a cosmetic composition having an anti-inflammatory effect that can be used safely without side effects on the skin and the skin trouble-relieving effect.

In order to solve the above technical problem, the present invention provides a cosmetic composition having the anti-inflammatory effect, characterized in that it comprises a ally extract, zymo extract, thyme extract and oat extract as an active ingredient.

The cosmetic composition of the present invention is safe to the skin and has an excellent anti-inflammatory effect, which is a skin trouble-relieving effect.

Hereinafter, the present invention will be described in more detail.

The cosmetic composition having the anti-inflammatory effect of the present invention is characterized in that it comprises a ally extract, zymo extract, thyroid leaf extract and oat extract as an active ingredient.

The stalk is the seed of the burdock (Arctium lappa L.), the zygote is the rhizome of the annemarrhena asphodeloides Bunge, the lateral lobe is the leaf of the Biota orientalis L., and the oat is the oat (Avena sativa L.) Is the seed.

The medicinal material may be prepared as an extract according to a conventional method, for example, distilled water, or an organic solvent such as anhydrous or water-containing lower alcohol having 1 to 4 carbon atoms and then concentrated under reduced pressure.

The stool extract, zygota extract, thyme leaf extract and oat extract are preferably included in the cosmetic composition of the present invention, respectively, at 0.0001 to 20% by weight, and more preferably, at 0.01 to 10% by weight. If the content is less than 0.0001% by weight, a clear effect cannot be expected.

The formulation of the cosmetic composition having an anti-inflammatory effect containing the above-mentioned ally extract of the present invention, zygota extract, thyme extract and oat extract as an active ingredient is not particularly limited, and may be appropriately selected according to the purpose. Specifically, the cosmetic composition may be prepared in a formulation such as softening, longevity, nourishing cream, massage cream, essence, pack, gel, lotion, ointment, patch or spray.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are only intended to illustrate the present invention and the scope of the present invention is not limited to the following examples.

[Example]

Examples 1 to 4

The dried shoots, jimo, side lobe and oats were each finely chopped. After 500 ml of ethanol was added to each fine 100 g of ginseng, zymo, hinoki, and oats, the cells were aged at room temperature for 7 days. Then, the extracts were sequentially filtered with a filter having a size of 0.4 μm, 02 μm, and 0.1 μm, and concentrated under reduced pressure to prepare extract concentrates of stool, zebra, thyme, and oat, respectively.

[Experimental Example]

Experimental Example 1. PGE ₂ production inhibitory effect

In order to confirm the skin trouble-relieving function of the anti-inflammatory effect of the allies extract, zygoma extract, thyme extract and oat extract prepared in Examples 1 to 4, a PGE2 production inhibitory effect test was conducted. (Jeffrey, K. H., Anglea, S. W., Zoe, S., and Rhia C. M., Intracellular Measurement of Prostaglandin E2: Effect of antiinflammatory drugs on cyclooxygenase activity and prostanoid expression, Analytical Biochemistry, 1999, 271, 18-28)

After macrophages RAW 264.7 cells were passaged several times, 1 x 10 ⁵ cells were placed in 24-well plates and cultured for 24 hours in a 5% CO ₂ thermostat. Thereafter, the medium was removed and treated with a new medium containing 50 µl of samples of Examples 1 to 4 prepared at 10-fold concentration (1 mg/ml) and 450 µl of LPS (1 µg/ml) at the same time, and under the same conditions as in the previous culture. Incubated in. To measure PGE ₂ after 24 hours, the culture medium was centrifuged (12,000 rpm, 3 min) to obtain a supernatant. The measurement of PGE ₂ was determined by enzymatic immunoassay (Enzyme-Linked Immunosorbent Assay, ELISA) to determine the prostaglandin inhibitory effect of herbal extracts and the results are shown in Table 1 below.

sample Inhibition rate (%) Control - Example 1 63.4 Example 2 65.8 Example 3 62.3 Example 4 52.7

As shown in Table 1, Examples 1 to 4 of the present invention showed a very strong prostaglandin (PGE ₂₎ inhibition rate, and thus it was found that the anti-inflammatory efficacy was very excellent.

[Production example]

Preparation Example 1 and Comparative Example 1

The ointment for external use on the skin was prepared according to the formulation of Table 2 using Example 1.

Composition weight% Preparation Example 1 Comparative Example 1 Extract of Example 1
Diethyl sebacate
Arrears
Polyoxyethylene oleyl ether phosphate
Sodium benzoate
vaseline 0.2
8
5
6
Proper
To 100 -
8
5
6
Proper
To 100

Preparation Example 2 and Comparative Example 2

Using Example 2, a cream was prepared according to the prescription in Table 3 below.

Composition weight% Preparation Example 2 Comparative Example 2 Extract of Example 2
Stearic acid
Cetane
Potassium hydroxide
glycerin
Propylene glycol
antiseptic
incense
Purified water 0.2
15
One
0.7
5
3
Proper
Proper
To 100 -
15
One
0.7
5
3
Proper
Proper
To 100

Preparation Example 3 and Comparative Example 3

Using Example 3, a softening makeup was prepared according to the prescription in Table 4 below.

Composition weight% Preparation Example 3 Comparative Example 3 Extract of Example 3
ethanol
Polylauric acid polyoxyethylene sorbitan
Methyl paraoxybenzoate
glycerin
13-butylene glycol
incense
Coloring
Purified water 0.1
10
One
0.2
5
6
Proper
Proper
To 100 -
10
One
0.2
5
6
Proper
Proper
To 100

Preparation Example 4 and Comparative Example 4

Using Example 4, the nutritional makeup was prepared by the prescription of Table 5 below.

Composition weight% Preparation Example 4 Comparative Example 4 Extract of Example 4
vaseline
Sesquiolein sorbitan
Polyoxyethylene oleyl ethyl
Methyl paraoxybenzoate
Propylene glycol
ethanol
Carboxyvinyl polymer
Potassium hydroxide
Coloring
incense
Purified water 0.2
2
One
One
Proper
5
3
18
0.1
Proper
Proper
To 100 -
2
One
One
Proper
5
3
18
0.1
Proper
Proper
To 100

[Experimental Example]

Experimental Example 2. Itching and atopic dermatitis improvement effect experiment

Among the patients diagnosed with atopic dermatitis, about 20 people who complained of severe itching were selected and the cosmetics prepared according to Preparation Example 2 and Comparative Example 2 were applied twice a day (4 weeks) to the affected area of both arms. The degree of itching and atopic dermatitis of the Preparation Example and Comparative Example were judged by the questionnaire and the naked eye, and the degree of itching and atopic dermatitis improvement of the Preparation Example was evaluated in three stages of distinct effect, effect, and no difference compared to the Comparative Example. It is shown in Table 10 below.

Test Items Pronounced effect (person) Effective (persons) No difference (person) Itching improvement 7 13 0 Atopic dermatitis improvement 4 14 2

As can be seen from the results in Table 6, the cosmetic preparations prepared according to Preparation Example 2 had a very excellent effect of improving itching and atopic dermatitis among 20 subjects, and did not show any side effects in the skin, so it was safe and very effective It can be seen that it is a cosmetic for improving itching and atopic dermatitis.

Claims (6)

A cosmetic composition having an itching improvement or anti-inflammatory effect, characterized in that it comprises an ally extract, zymo extract, thyroid leaf extract and oat extract as an active ingredient. According to claim 1,
Cosmetic composition, characterized in that the weight ratio of the stool extract: jimo extract: lateral leaf extract: oat extract is from 1: 0.1 to 10: 0.1 to 10: 0.1 to 10. The method according to claim 1 or 2,
The itching is cosmetic composition, characterized in that itchy scalp or atopic dermatitis. The method according to claim 1 or 2,
The extract is a cosmetic composition, characterized in that extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohol or a mixture thereof. According to claim 3,
The extract is a cosmetic composition, characterized in that extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohol or a mixture thereof. According to claim 1, The content of the active ingredient is a cosmetic composition having an anti-inflammatory effect, characterized in that 0.00001 to 20% by weight based on the total weight of the composition.