KR20200083022A - A method of preparing peptide from rice bran - Google Patents

Abstract

The present invention relates to a protein preparation method for protein extraction from rice bran. The method includes: a step of stabilizing embryo bud-removed rice bran; a rice bran powder preparation step of grinding the stabilized rice bran; a protein extraction step of adding 800 parts by weight of water to 100 parts by weight of the rice bran powder, adjusting the pH to 10, performing stirring for 60 minutes at 15 to 20 degrees Celsius, performing centrifugation for 30 minutes at 3,000 rpm, and then taking the supernatant; a protein acquisition step of adjusting the pH of the supernatant to 6, leaving it for 30 minutes at 15 to 20 degrees Celsius, performing centrifugation for 30 minutes at 3,000 rpm, and then acquiring the precipitate; and a protein washing step of performing water washing by adding distilled water to the acquired protein, performing centrifugation, and then acquiring the precipitate. The present invention corresponds to a simple and efficient method for preparing rice bran-derived protein. Accordingly, a large quantity of natural protein compositions can be effectively prepared from rice bran and the present invention can be widely used in a food industry using natural protein or a natural amino acid peptide group as well as an industry related to functional food.

Description

Protein production method for extracting protein from rice bran{A METHOD OF PREPARING PEPTIDE FROM RICE BRAN}

The present invention relates to a protein production method for extracting proteins with excellent efficiency from rice bran.

Essential amino acids are not produced by our body, so we must consume a certain amount of food daily within the recommended daily dose range. However, the content of essential amino acids among proteins absorbed through everyday food varies depending on the type of food and has different requirements for each individual according to the dietary pattern. Protein is the most important nutrient that our body needs. Of course, all nutrients are important for normal physiological activity, but protein is the most important nutrient. Protein is an enzyme that acts as a catalyst for vital activity in our body, as a hormone, as a component of muscles or nerves, and is a substance that plays many important roles, such as red blood cells, which provide oxygen.

Until now, milk, which is well known as a high protein complete food, contains about 3.4% of protein. About 80% of these are composed of a protein called casein, and the rest are made of whey protein. Whey proteins include several water-soluble proteins such as lactoalbumin, lactoglobuline, serum albumin, and immunoproteins. The protein of milk is an important ingredient with a very high nutritional function that provides a lot of essential amino acids that must be consumed by the digestion in the stomach and intestines as well as the biological function of each protein. In addition, since the digestibility of milk protein is known to be more than 97%, it is known as a protein that is more digestible than vegetable proteins such as soybeans.

On the other hand, rice bran is one of the vast amounts of biomass produced worldwide by mankind every year. Korea's rice production is estimated at about 7.3 million tons, and the production of rice bran is estimated at about 580,000 tons. Currently, rice bran is partly used for the production of rice bran oil, but part of it is used as animal feed and is mostly discarded without special use. Rice bran is known to contain about 34 to 52% soluble sugar, 15 to 20% crude fat, 11 to 15% crude protein, 7 to 11% crude fiber, 7 to 10% ash, and 14% starch. In particular, the protein content of rice bran shows a higher content than milk protein. In particular, rice bran protein is known to be hypoallergenic than other proteins. In addition, rice bran is rich in vitamins such as dietary fiber, including hemicellulose, inositol, choline, niacin (B3), tocopherol (E), thiamin (B1), and pantothenic acid (B5). have. In addition, rice bran is known to contain excessive amounts of nutritionally rich useful ingredients, but until now, it has been used only as a medium ingredient for animal feed and mushroom cultivation. It has not been developed as a source of abundant protein.

The reason for this is, firstly, the complex structural features of rice bran protein. Rice bran protein contains 37% albumin, 36% globulin, 22% glutelin and 5% prolamin. Second, rice bran protein has a very low solubility. This is because rice bran protein is strongly agglomerated and has a strong disulfide bond crosslink. Third, rice bran protein contains high phytate (1.7%) concentration and fiber content (12%). This structure provides a very difficult point in extracting and extracting protein components.

In recent years, as the national economy has developed and the national income has gradually increased, the population of the elderly has continued to increase, and the majority of people have shown considerable interest in health. I am going. Therefore, it is very meaningful to develop a stepwise extraction of nutritionally rich useful ingredients from rice bran that can be supplied from nature at a low price with poor utilization.

(Prior literature 1) KR 10-1851137B (Prior Art 2) KR 10-1628783B (Prior Art 3) KR 10-0904977B (Prior Art 4) KR 10-0503100B

The present invention is to improve the problems of the prior art as described above, a method for producing a protein from rice bran that can extract nutrients rich in rice bran, that is, protein components with excellent efficiency, and the protein produced by the manufacturing method It aims to provide.

In order to achieve the above object, the present invention provides a protein production method for extracting protein from rice bran.

Protein production method for extracting protein from the rice bran of the present invention is to add water to the rice bran powder, adjust the pH to pH 10, stir for 60 minutes at 15 to 20° C., centrifugate, and take a supernatant; And after adjusting the pH of the supernatant to pH 6, it may be to stand for 30 minutes at 15 to 20 ℃ and centrifugation to obtain a protein to obtain a precipitate.

In the protein extraction step, preferably, 800 parts by weight of water is added to the rice bran powder based on 100 parts by weight of the rice bran powder, the pH is adjusted to pH 10, stirred at 15 to 20°C for 60 minutes, and then 30 at 3,000 rpm. After centrifugation for a minute, it may be performed by taking a supernatant.

The step of obtaining the protein may be preferably performed by adjusting the pH of the supernatant to pH 6, leaving it at 15 to 20°C for 30 minutes, and then centrifuging at 3,000 rpm for 30 minutes to obtain a precipitate. have.

In addition, the protein production method for extracting protein from the rice bran may further include a step of stabilizing the rice bran and a step of preparing the rice bran powder to obtain the rice bran powder from the stabilized rice bran.

The step of stabilizing the rice bran may be performed by a method of stabilizing the rice bran by treating the rice bran with removed rice bran in an oven at 120° C. for 10 minutes, and then cooling at 0 to 4° C. for 5 minutes.

In addition, the step of producing the rice bran powder may be performed by pulverizing the stabilized rice bran using a grinder, and then passing through a 100 mesh sieve to obtain the passed rice bran powder.

In this aspect, the protein production method for extracting protein from the rice bran of the present invention comprises the steps of stabilizing the rice bran; Rice bran powder manufacturing step; Protein extraction step; Protein obtaining step; And protein washing steps.

The step of stabilizing the rice bran may be performed by treating the rice bran with the removed rice eyes in a 120° C. oven for 10 minutes and then cooling at 0 to 4° C. for 5 minutes.

The step of preparing the rice bran powder may be performed by pulverizing the stabilized rice bran using a grinder, and then passing through a 100 mesh sieve to obtain the passed rice bran powder.

In the protein extraction step, 800 parts by weight of water is added to the rice bran powder based on 100 parts by weight of the rice bran powder, the pH is adjusted to pH 10, stirred at 15 to 20°C for 60 minutes, and then at 3,000 rpm for 30 minutes. After centrifugation, it can be carried out by taking a supernatant.

The step of obtaining the protein may be performed by adjusting the pH of the supernatant to pH 6, leaving it at 15 to 20°C for 30 minutes, and then centrifuging at 3,000 rpm for 30 minutes to obtain a precipitate.

The protein washing step may be performed by adding distilled water to the obtained protein, washing with water, and centrifuging to obtain a precipitate.

The protein production method for extracting protein from the rice bran of the present invention is limited in its use, and can extract high-quality protein essential for our body from the rice bran used only as an energy source (BIOMASS) or feed. Since it is possible to extract proteins with high yield in a very effective way, it is possible to produce useful physiological substances from agricultural waste resources in this way. It will contribute to development, and the industrial effect will be very great.

1 is a flow chart for a method for producing a protein from the rice bran of the present invention according to an embodiment of the present invention.
2 is a photograph of an alkaline aqueous solution in an extraction step of performing protein extraction according to an extraction pH condition according to an embodiment of the present invention, and FIG. 3 shows extraction pH and precipitation pH according to an embodiment of the present invention. As a graph confirming the protein concentration, the horizontal axis represents the extraction and precipitation conditions, and the vertical axis represents the protein content (g) in the extract.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the scope of the present invention is not limited by the following examples.

< Example 1> Measurement of protein content extracted from rice bran according to extraction conditions

For rice bran samples, rice bran obtained from rice cultivation farms in Muan-gun, Jeollanam-do, was used.

First, after removing rice husk using a 10 mesh sieve, the separated rice bran was sieved twice with a 35 mesh sieve to separate rice eyes and impurities. In order to stabilize the rice bran from which the rice eyes were removed, the pan was spread to a thickness of 0.1 to 1.5 cm, then treated in an oven at 120°C for 10 minutes, and immediately cooled in a freezer for 5 minutes to stabilize. Stabilized rice bran was crushed with a grinder (DA 282-2, Daesung Artron, Korea) and passed through a 100 mesh (< 150 μm) sieve to produce rice bran powder and stored in a freezer.

The protein extraction process from rice bran was performed by the method shown in FIG. 2.

First, 24 mL of distilled water was added to 3 g of each rice bran, and the pH was adjusted to 8, 9, 10, 11 and 12 using 1N NaOH. The mixture was stirred at room temperature for 60 minutes, centrifuged at 3,000 rpm for 30 minutes, and then the supernatant was taken. The stirring solution before centrifugation according to each pH condition is shown in FIG. 2.

In order to compare the degree of protein extraction according to the isoelectric point precipitation pH of the protein derived from rice bran extracted under various alkali conditions, each precipitation pH per elution pH was adjusted to 2, 4 and 6 to precipitate. Specifically, the protein fraction extracted under the alkaline conditions was adjusted to pH 2, 4 and 6 using 1N HCl, left at room temperature for 30 minutes, and then centrifuged at 3,000 rpm to obtain a precipitated protein. The obtained precipitated protein was washed several times by adding 5 mL of distilled water, and then centrifuged again to obtain a precipitate, and distilled water was added to homogenize sufficiently. The homogenized aqueous protein solution was neutralized to pH 7, and was used to measure the protein content of the obtained aqueous protein solution.

The content of eluted and precipitated proteins was measured by Bradford method. First, the obtained sample was diluted 10-fold to react for 10 minutes by mixing Bradul reagent 500ul and sample 10ul, and absorbance was measured at 595nm. At this time, the protein content was calculated using a calibration curve prepared with Bovine serum albumin (BSA) as a standard product. Fig. 2 shows the calculated result.

As shown in FIG. 2, extraction yields of various forms were confirmed according to the conditions of the extraction pH and the conditions of the precipitation pH, respectively. No regularity was found in these conditions, and after extraction under the condition of pH 10, pH 6 Precipitation under conditions of was found to be about 300% to 50% better than other conditions.

Claims (4)

Stabilizing the rice bran by removing the rice bran from the rice bran treated at 120° C. for 10 minutes, and then cooling at 0 to 4° C. for 5 minutes; After crushing the stabilized rice bran using a grinder, a rice bran powder production step of passing through a 100 mesh sieve to obtain the passed rice bran powder; After adding 800 parts by weight of water based on 100 parts by weight of the rice bran powder to the rice bran powder, adjusting the pH to pH 10, stirring at 15 to 20°C for 60 minutes, and then centrifuging at 3,000 rpm for 30 minutes, Protein extraction step of taking the supernatant; After the pH of the supernatant was adjusted to pH 6, left at 15 to 20°C for 30 minutes, and then centrifuged at 3,000 rpm for 30 minutes to obtain a protein to obtain a precipitate; And washing with water by adding distilled water to the obtained protein, followed by centrifugation to obtain a precipitate.
Protein production method for extracting protein from rice bran containing. After adding water to the rice bran powder, adjusting the pH to pH 10, stirring at 15 to 20°C for 60 minutes, centrifuging, and then extracting the protein by taking a supernatant; And after adjusting the pH of the supernatant to pH 6, standing at 15 to 20°C for 30 minutes and centrifuging to obtain a protein to obtain a precipitate.
Protein production method for extracting protein from rice bran containing. According to claim 2,
In the protein extraction step, 800 parts by weight of water is added to the rice bran powder based on 100 parts by weight of the rice bran powder, the pH is adjusted to pH 10, stirred at 15 to 20°C for 60 minutes, and then centrifuged at 3,000 rpm for 30 minutes. After separation, characterized in that it is carried out by taking a supernatant,
The step of obtaining the protein is performed by adjusting the pH of the supernatant to pH 6, leaving it at 15 to 20°C for 30 minutes, and then centrifuging at 3,000 rpm for 30 minutes to obtain a precipitate.
Protein production method for protein extraction from rice bran. According to claim 2,
Stabilizing the rice bran by removing the rice bran from the rice bran treated at 120° C. for 10 minutes, and then cooling at 0 to 4° C. for 5 minutes; And crushing the stabilized rice bran using a grinder, further comprising a rice bran powder production step of passing through a 100 mesh sieve to obtain the passed rice bran powder.
Protein production method for protein extraction from rice bran.

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