KR20200077684A - Flow Control type continuous microalgae cultivating device and cultivating method thereof - Google Patents

Abstract

The present invention relates to a flow control type continuous microalgae cultivating device. The continuous cultivating device manufactured to cultivate microalgae includes a partition wall means, and a cultivating unit including a cultivating chamber in which cultivating spaces are divided for each cultivating process of microalgae by the partition wall means. When the cultivation is completed step by step, the microalgae are moved from one cultivating space to the next cultivating space by the partition wall means to be provided with a next cultivation stage, and cultivation stages are subdivided according to the microalgae to provide n number of cultivating spaces.

Description

Flow control type continuous microalgae cultivating device and cultivating method thereof

The present invention relates to a continuous microalgae culture apparatus, and more specifically, the culture chambers are divided and installed into partition walls that can be opened and closed in each culture step, and when the culture chambers are inclined for each culture step, the culture walls are sequentially opened, and then each culture chamber is opened. It relates to a flow-control-type continuous microalgae culture apparatus and a culture method for allowing the microalgae contained in the flow to the next culture chamber.

In general, microalgae are the oldest living organisms on the planet, reaching thousands of species, but about 0.1% of them are known for their physiological activity, and only a few are cultivated on an industrial scale, including chlorella and spirulina. Microalgae such as (spirulina) are being developed as a variety of material materials such as dietary supplements, health supplements, aquaculture feeds, alternative medicines and energy resources.

Meanwhile, physiologically active substances produced by plants are secondary metabolites, and are known to have antioxidant, anti-inflammatory, anti-fungal, anti-cancer and anti-cancer activities that are produced to defend themselves from the outside after cell growth is completed. Carotenoids produced from plants are also representative secondary metabolites, and cases produced from microalgae have been reported. In addition, it is known that the production of physiologically active substances produced from microalgae is produced in the resting period, where growth is stopped, rather than the logarithmic growth period of the strain. For maximum production of physiologically active substances, primary culture to maximize cell culture in logarithmic period, It is essential to construct a two-stage growth of secondary culture that maximizes the production of bioactive substances using the maximum number of cells during the resting period. Therefore, the culture conditions for seed inoculation are greatly influenced by osmotic pressure and shear stress, and culture conditions for minimizing the impact on cells should be provided. In view of this, development of a continuous flow type culture apparatus is actively being conducted.

Briefly described with respect to such a conventional culture device, as shown in Figure 1, a plurality of first vertical pipe 10, a plurality of first vertical pipe 10 to supply microalgae to the bottom of the first vertical pipe 10 The first horizontal pipe 20 installed in the second vertical pipe 30 so that a plurality of second vertical pipes 30 and a plurality of second vertical pipes 30 disposed adjacent to the first vertical pipe 10 pass through ), the second horizontal pipe 40 installed at the lower end, and any one of the first vertical pipe 10 and the second vertical pipe 30 that are adjacent to each other. Accordingly, the microalgae supplied from the first horizontal tube 20 are supplied to the first vertical tube 10, and the microalgae cultured in the first vertical tube 10 is the second horizontal tube through the connection pipe 50. It may be supplied to the (40) and the second vertical pipe (30). Thereafter, when the culture is completed, it may be discharged through any one of the second vertical pipes 30.

However, in order to supply the initial microalgae to the first horizontal tube 10, to move the microalgae from the first vertical tube 10 to the second vertical tube 20, or to discharge the microalgae in which the culture is completed Since a transfer device including a pump is required, there is a problem that a manufacturing and maintenance cost of the culture device is high.

In addition, there is another problem that the quality of the final product is lowered and mass production through real-time control is difficult due to the collective movement and discharge of the microalgae of each vertical tube while ignoring it.

Republic of Korea Registered Patent No. 10-1721448 (Announcement of March 30, 2017.) Republic of Korea Registered Patent No. 10-1567140 (announced on Nov. 09, 015) Republic of Korea Registered Patent No. 10-0585216 (20006.06.08. Announcement) Republic of Korea Patent Publication No. 10-2013-0063276 (published on June 14, 2013)

The object of the present invention was devised to solve the above-described problem is that a number of culture chambers in which the surface area of the bottom increases in accordance with the cultivation stage of the microalgae are sequentially arranged, and when the culture chambers are inclined and the barrier ribs are opened sequentially, gravity is applied. It is to provide a flow-control type continuous microalgae culture apparatus and a culture method that is manufactured to enable mass production through step-by-step culture by moving the microalgae being cultured in the culture chamber to the next culture chamber.

In order to achieve the above object, the flow-controlled continuous microalgae culture apparatus according to the present invention is a continuous culture apparatus designed to cultivate microalgae, a partition wall means, and the culture spaces of the microalgae are cultured by the partition wall means. It may include; a culture unit having a culture chamber divided by each.

At this time, the partition wall means is installed to open and close the adjacent culture spaces by elevating the lifting bulkhead to move the microalgae of the culture space to the next culture space, or by pushing the microalgae while moving the moving bulkhead, the next culture in the culture space It can be installed to move to the location of the space.

In addition, the floor spaces of the culture spaces may be different for each culture process.

In addition, the partition wall means is arranged to be adjacent to the lifting partition installed to elevate while being divided into a plurality of culture spaces, and may further include a moving member installed to push the microalgae of the culture space to the next culture space.

In addition, while the culture chamber is seated, one side of the culture chamber may be further provided with a base installed to rotate axially; and a lifting means installed to axially rotate the culture chamber; At this time, the microalgae of the culture space can be moved to the next culture space by gravity.

The partition wall means may further include a magnetic body installed to move the moving partition wall arranged to be divided into a plurality of culture spaces by magnetic force.

At this time, the moving bulkhead and the magnetic material may be installed to move the microalgae by culture process, and when the microalgae are discharged, the first culture space may be moved.

On the other hand, the method for culturing a flow-controlled continuous microalgae according to the present invention is a method of culturing microalgae in a plurality of culture spaces step-by-step, in which the microalgae inoculated with seed germs are first cultured in a culture chamber by a partition wall means. Supply step of supplying; A moving and feeding step of moving the microalgae of the first culture space to the next culture space, and supplying the microalgae inoculated with the seed bacteria to the first culture space; It may include a repeating step of repeating the moving and supplying step; and the discharge step of discharging the microalgae that have been cultured in the final culture space.

At this time, the partition wall means sequentially raises and lowers the partition wall so that adjacent culture spaces pass sequentially, and the moving member is reciprocated to move the microalgae of the culture space to the next culture space or the microalgae as the moving bulkheads move. It can be moved to the next culture space by pushing.

In addition, the culture chamber is inclined, and the lifting bulkhead is raised to allow the microalgae to move from the culture space to the next culture space by gravity.

In addition, the moving bulkhead may contact the bottom of the culture chamber by a magnetic material to move or slide.

According to the present invention as described above, the initial growth of the inoculation, logarithmic growth period, at least three of the secondary metabolite production, provided with six culture spaces, for example, by providing a lighting means for each culture space, optimal for each culture space It has the effect of providing illuminance, wavelength and temperature.

In addition, by providing the LED module of a complex structure in which the LEDs for each wavelength are combined with the lighting means, the light source and cells necessary for the growth of microalgae are provided by changing and providing the optimal wavelength for each culture process. It has the effect of measuring growth, temperature, humidity, carbon dioxide, illuminance, wavelength, etc. to maintain an optimal state at all times.

In addition, when the culture is completed step by step, the microalgae are moved from the culture space to the next culture space by the partition means, and the culture of the next stage is provided, and the culture stage is subdivided according to the microalgae to provide n culture spaces. There is an effect that can be provided.

In addition, in the case of the moving bulkhead and the magnetic material, the size of the bottom surface area of the culture space can be arbitrarily changed to maximize production.

The following drawings attached in this specification are intended to illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the detailed description of the present invention, so the present invention is limited only to those described in those drawings. And should not be interpreted.
1 is a schematic view showing a conventional culture apparatus.
2 is a side view schematically showing a flow control type continuous microalgae culture apparatus according to a preferred embodiment of the present invention.
3 is a perspective view of the culture chamber shown in FIG. 2.
Figure 4 is a side view showing the operating state of the culture device shown in Figure 2;
5A to 5C are side views schematically illustrating a flow-controlled continuous microalgae culture apparatus according to another embodiment.
6 is a block diagram showing a method of culturing microalgae using the culture apparatus of FIG. 2.
7 to 10 is a view schematically showing the process of microalgae is abbreviated in the culture method shown in FIG. 6.

Hereinafter, preferred embodiments will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains can easily implement the present invention. However, in the detailed description of the operating principle of the preferred embodiment of the present invention, if it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

<Configuration>

In the flow-controlled continuous microalgae culture apparatus according to the present invention, a plurality of partitioning means are arranged at regular intervals in one culture chamber, and a plurality of culture spaces are provided, and microalgae can be proliferated in each culture step in each culture space. In one example, the partition wall means may include a plate-like and elevating partition, and a plate-shaped and movable member reciprocating in the left and right directions. In addition, as another example, the partition wall means may include a cylindrical or inverted "T" shape, a moving partition wall that moves or slides, and a magnetic body that moves the partition wall with magnetic force.

When the step-by-step propagation is completed, the culture chamber may be positioned to be inclined to move the microalgae from the culture chamber of a certain culture step to the culture chamber of the next culture step through reciprocating movement of the moving member with gravity. At this time, the culture chamber is provided with at least 4 or more culture spaces to cultivate the microalgae inoculated with seed germs, propagate the microalgae, and induce the proliferated microalgae into a resting period to generate a physiologically active substance as a secondary metabolite. Can be.

In addition, to briefly describe the culture process and method of an embodiment, the microalgae inoculated with the seed bacteria may be supplied to the primary culture space and primary culture for a certain period of time. After the primary culture, when the culture chamber is inclined, the lifting bulkhead is opened, and as the moving member reciprocates, the primary cultured microalgae can be moved from the primary culture space to the secondary culture space. Thereafter, the lifting bulkhead is closed, and the culture chamber is returned to the horizontal, and then the microalgae inoculated with the seed bacteria in the primary culture space may be supplied. After repeating such an operation or process, the microalgae move to the last culture space, and when the culture is finished, the culture chamber is inclined, and when the lifting bulkhead is opened, the moving member reciprocates and the fully cultured microalgae can be harvested. Of course, the lifting bulkhead is closed again, the operation and process of transferring the microalgae of the previous culture chamber to the next culture chamber is repeated, and by supplying the microalgae inoculated with the seed into the initial culture space, the microalgae are continuously cultured and Can be harvested. This operation or process can be repeated to cultivate microalgae in large quantities and harvest.

To briefly describe the culture process and method of another embodiment, a plurality of moving bulkheads and magnetic bodies are spaced apart from each other in a culture chamber to provide culture spaces, and microalgae inoculated with seed germs are supplied to the first culture space for a first period of time. Can be cultured. After the secondary culture, the moving bulkhead and the magnetic bodies move, so that the microalgae can be moved to the secondary culture space. At this time, another moving bulkhead and a magnetic body are disposed inside the culture chamber to prepare a primary culture space, and microalgae inoculated with seed bacteria may be supplied to the primary culture space. This operation and process is repeated, and when the last culture is completed, the moving bulkhead and the magnetic body may be moved to discharge microalgae to the outside of the culture chamber. By repeating this process, microalgae can be cultivated and harvested.

In addition, when the microalgae are cultured in the culture space of each stage, the cells proliferate, so that the culture space of the next culture stage may have a surface area of approximately 5 to 20% wider than the culture space of the previous culture stage. In addition, various culture conditions, including light source, illuminance or illumination amount, temperature, carbon dioxide, acidity and electrical conductivity (ph/Ec), may be adjusted and supplied in an optimal state according to the culture step for each culture space.

<Example>

The flow control type continuous microalgae culture apparatus according to a preferred embodiment of the present invention includes a base 1000, a culture unit 2000, and a lifting means 3000, as shown in FIGS. 2 and 4.

First, the base 1000 is a culture unit 2000 is seated, the lifting means 3000 for lifting the culture unit 2000 is installed. At this time, the base 1000 may be installed such that one portion of the culture unit 2000 is coupled to the rotation shaft 1100, and the culture unit 2000 is rotatable about the rotation shaft 1100.

On the other hand, the culture unit 2000 may be configured to generate a physiologically active substance, which is a secondary metabolite, through the growth stage of the logarithmic growth phase of the microalgae inoculated with the seed germ. The culture unit 2000 may include a culture chamber 2100 and a lighting means 2200.

Here, the culture chamber 2100, as shown in Figures 2 and 3, is manufactured to accommodate and culture the microalgae in the culture step. The culture chamber 2100 may be in the form of an approximately long length water tank. One side of the discharge portion may be installed to open and close the culture space 2110 by rotating the upper part in an axis to discharge the microalgae which have been cultured to the outside. The culture chamber 2100 may include a plurality of culture spaces 2110 and a partition wall means 2120.

The culture space 2110 may be provided in a number to accommodate microalgae in each culture stage. These culture spaces 2110 are used for three stages of culture, such as primary culture of microalgae inoculated with seed, secondary culture for propagating microalgae, and tertiary culture for producing bioactive substances as secondary metabolites. At least four or more may be provided. As an example, as shown in FIGS. 2 and 4, the culture space 2110 is provided with six of the sixth culture space 2116 in which the microalgae are finally cultured in the first culture space 2111 in which the first microalgae are cultured. Can. The culture space 2110 may be provided as necessary.

In addition, the cultivation spaces 2110 have a large surface area as the primary culture, the secondary culture, and the tertiary culture of the microalgae progress, which can be adjusted according to the installation position of the partition means 2120. For example, as the area from the first culture space 2111 to the sixth culture space 2116 increases, the bottom surface area may be increased by approximately 5 to 20%. This is to provide conditions for more cells to proliferate as they are cultured. In addition, the bottom surface area may be different for each culture space 2110 and may be different for each set.

The partition wall means 2120 may include a lifting partition wall 2121 and a moving member 2122.

The lifting bulkhead 2121 may be installed to divide the culture chamber 2100 into three or more or six, so that a plurality of culture spaces 2110 are provided. The elevating partition 2121 may be installed to elevate along the guide groove on the inner surface of the culture chamber 2100 to block or open neighboring culture chambers 2100. Therefore, when the inclined partitions 2121 descend and each culture space 2110 is blocked, the flow of microalgae in each culture space 2110 is blocked, so that the culture can proceed in each culture space 2110. In addition, when the lifting bulkheads 2121 are sequentially raised and each culture space 2110 is sequentially opened, microalgae may move from the culture space 2110 of the previous culture step to the culture space 2110 of the next culture step. At this time, the culture space 2110 may be inclined with the culture chamber 2100 inclined by the lifting means 3000 so that the microalgae move by gravity. In addition, while the neighboring previous culture space 2110 and the next culture space 2110 are opened, the moving member 2122 moves toward the next culture chamber 2100, and the microalgae of the previous culture chamber 2100 are completely cultured next. When pushed to the chamber 2100, the lifting bulkhead 2121 may descend to be installed to block the previous culture space 2110 and the next culture space 2110. For example, as shown in FIG. 2, the first and second partition walls 2121 to 6121 may be installed on the upper and lower partition walls 2121. In addition, the lifting bulkhead 2121 may be installed to descend to open the culture chamber 2100, and rise to close the culture chamber 2100.

The moving member 2122 may be installed to forcibly move the microalgae that may remain in the previous culture space 2110 according to the inclination angle of the culture chamber 2100 to forcibly move to the next culture space 2110. The moving member 2122 may be installed to reciprocate from one side of the lifting bulkhead 2121 to the other side of the lifting bulkhead 2121. For example, it may be installed to come into contact with one side of the lifting bulkhead 2121 and returning after moving to the other side of the lifting bulkhead 2121. The moving member 2122 may have a plate shape, and the lower portion may be refracted or a separate member may be attached to the lower portion to facilitate movement while sweeping the bottom of the culture chamber 2100. In addition, the moving member 2122 may be installed for every six culture spaces 2110 as an example, and may be installed for every five culture spaces 2110 excluding the last culture space 2110.

In addition, the lighting means 2200 may be installed in each culture space 2110 to provide lighting in accordance with set conditions including appropriate illumination and amount of illumination for cultivation of microalgae. The lighting means 2200 may be installed on the top and/or side and/or bottom surfaces of each culture space 2110, and may include a light source including LEDs. For example, the lighting means 2200 may include first light sources 2210 to sixth light sources 2220 installed in each of the first culture space 2111 to the sixth culture space 2116. The first light source 2210 to the sixth light source 2220 may provide different optimal lighting conditions according to the culture step of each culture space 2110. As an example, the number of LEDs may be further installed such that the illuminances of the first light sources 2210 to 6220 are sequentially increased by approximately 5 to 20%. In addition, the first light source 2210 to the sixth light source 2220 may be installed on a panel disposed on the top surface of the culture chamber 2100, or may be installed on the side surface of the culture chamber 2100, or may be installed on the bottom surface of the culture chamber. have.

In addition, the luminaire 2200 may be provided with a composite structure LED module in which LEDs for each wavelength are combined to control wavelength and illuminance optimized for microalgae growth by process by manufacturing LED designs and modules. In addition, the heat generated from the LED may be configured in a manner of controlling in conjunction with cooling water for temperature control in order to save energy.

On the other hand, the lifting means 3000 may be installed to rotate the culture chamber 2100. 2 and 4, as shown in FIGS. 2 and 4, two or more of the lifting means 3000 are disposed at a lower portion of the culture chamber 2100 at regular intervals, and the culture chamber coupled to the base 1000 by a rotating shaft 1100. It can be installed to rotate the (2100). At this time, the inclination of the culture chamber 2100 does not overflow the microalgae while the entire amount of microalgae is pushed from the culture space 2110 to the next culture space 2110 by the opening and moving member 2122 of the lifting bulkhead 2121. It may not be. The lifting means 3000 may be a general variety of devices including a cylinder and a cylinder rod, and may be installed at the lower or upper or side of the culture chamber 2100.

Meanwhile, the culture apparatus according to the present invention may further include a plurality of sensors installed to measure various culture conditions in each culture space 2110. The outputs of these sensors can be collected through the multi-channel DAC board as analog and digital outputs, and an optimized culture environment can be realized by controlling light sources, cell growth, temperature, humidity, carbon dioxide, illuminance, and wavelength according to the information. .

<Other embodiments>

Hereinafter, the flow control type continuous microalgae culture apparatus of the present invention according to another embodiment will be described in comparison with the embodiment of FIG. 2 with reference to FIGS. 5A to 5C.

5A and 5B, the culture unit 2000 includes a culture chamber 2100 in which front and rear surfaces are opened and closed, and a partition wall means 2120 installed to provide a plurality of culture spaces 2110 in the culture chamber 2100. It may include.

The culture chamber 2100 may be installed such that one surface of the sixth culture space 2116 rotates axially to discharge the microalgae pushed by the partition wall means 2120 to the outside. In addition, the culture chamber 2100 may be installed such that the front surface of the first culture space 2111 side and the rear surface of the sixth culture space 2116 side axially rotate for the movement of the endless orbit of the partition means 2120. have.

The partition wall means 2120 includes a moving partition wall 213 disposed in the culture chamber 2100 to divide the culture space 2110, and a magnetic body 2124 disposed on the outer surface of the culture chamber 2100 to move the moving partition wall 213 ).

Here, the movable partition wall 213 may be various shapes for dividing the culture space 2110, including a cylindrical shape, an inverted “T” shape, and a panel shape, and steel (movable by the magnetic force of the magnetic body 2124)鋼鐵) It can be material. At this time, the cylindrical shape reduces the friction with the bottom of the culture chamber 2100 and facilitates rolling or sliding motion, while the inverted "T" shape reduces the weight while increasing the magnetic force area with the magnetic body 2124 and reduces the weight. The frictional resistance with the bottom of (2100) can be reduced. In addition, the movable partition wall 213 and the magnetic body 2124 may be set one by one, and may be installed as many as the culture space 2110. In addition, the moving partition wall 213 may be arranged to move together by magnetic force when the magnetic body 2124 moves. In addition, as shown in the drawing, the partition wall means 2120 is installed to move in an infinite track, and for this purpose, the front and rear surfaces of the culture chamber 2100 can be axially rotated flat and then returned to the original position. At this time, the movable partition wall 213 may be installed to move while maintaining the watertightness with the culture chamber 2100.

In addition, the magnetic body 2124 may be an electromagnet, and may be installed to move in a caterpillar form separately from the moving bulkhead 213. As shown in FIG. 5C, the magnetic body 2124 may be installed to move by an iron plate 2125 which is installed and moved in the form of a caterpillar at the bottom.

In addition, the partition wall means 2120 may be installed to move each of them, so that the area of each of the culture spaces 2110 can be easily changed as necessary. In addition, the movable partition wall 213 and the magnetic body 2124 may be installed to move in one direction according to the culture step. Therefore, the partition wall means 2120, when the cultivation step is completed at the corresponding position, pushes the microalgae to the next cultivation step position, and when the final cultivation is completed, pushes the microalgae and discharges it to the outside through the open rear surface of the culture chamber 2100. It can be moved to let. For example, when the culture is over and the partition wall means 2120 moves by a predetermined distance, the first culture space 2111 may move to the position of the second culture space 2112 to become the second culture space 2112. have. As such, the partition wall means 2120 may be installed to move the microalgae together with the culture space so that the next cultivation step proceeds, and when the final cultivation step ends, the microalgae are pushed out of the culture chamber 2100. In addition, the moving bulkhead 213 and the magnetic body 2124 from which the microalgae are discharged may be arranged to move in an orbital shape to provide a first culture space 2111.

<Cultivation conditions>

Hereinafter, the culture conditions for culturing the microalgae in the flow-controlled continuous microalgae culture apparatus according to a preferred embodiment of the present invention will be described. And in the case of the numerical values presented below, there may be an error range of about 5 to 20% even if it is not timely.

The culture apparatus according to the embodiment of the present invention may be manufactured to culture the microalgae about 20 L/day. For example, when the receiving area of one culture chamber 2100 is 50 × 20 × 20 cm, microalgae of a height of about 10 cm may be cultured, and approximately 20 L of microalgae may be cultured. If the three chambers are successively blocked in the case of the culture chamber 2100 blocked by the lifting partition 2121 or the moving partition 213, the receiving area may be 50×80×20 cm. In addition, in the case of the culture chamber 2100 in which six chambers are successively blocked by the elevating partition 2121 or the moving partition 213, the chamber may be 50×140×20 cm, and the nine chambers are continuously connected to the elevating partition 2121 or In the case of the culture chamber 2100 blocked by the moving partition wall 213, it may be 50×2000×20cm. Of course, the size of the culture chamber 2100 may vary according to the culture amount of the microalgae.

In addition, the control unit measures six types of monitoring functions in real time for each culture chamber 2100, such as cell volume, temperature, cell volume, temperature, acidity (pH), carbon dioxide (CO ₂ ), illuminance, wavelength, etc. It can include four types of control functions to control carbon dioxide (CO ₂ ), temperature, roughness, and acidity (pH) in real time by analyzing the values. As an example, the temperature is allowed to operate in the range of ±0.5 ℃ of the reference value suggested for the culture conditions, the acidity (pH) is ±0.5 of the reference value, carbon dioxide (CO ₂ ) is ±0.5% of the reference value, the illuminance is the reference It can be set to operate within a range within ±2% of the value (lux). Various conditions including this are described in [Table 1] below.

division Development content Specifications Light source By using a method of mixing sunlight and LED modules suitable for growth by using optical fibers or lenses that integrate sunlight, the optimal illumination and wavelength according to the step-by-step growth are the same. Solar wavelength: 2000 nm to 10000 nm
LED wavelength: 280 nm ~ 800 nm Roughness In order to maintain proper illumination, sunlight adjusts the light filter and, in the case of LED lights, adjusts the current value applied. Measuring range: 500 ∼ 10000 lux
Precision: ±0.5 Temperature In order to control temperature according to the growth conditions, heat pads and heat generated from LEDs are mixed to increase the efficiency of the system. Resolution: 0.1 ℃
Measuring range: -40 ℃ ~ + 65 ℃
Precision: ±0.5℃ Dioxide
carbon Carbon dioxide measurement and control for microalgae growth
Carbon dioxide measurement and control for microalgae growth Measuring range: 0 to 3000 ppm
Accuracy: ±0.5% ppm
(0 ~ 3000 ppm) @10 ~ 50℃
Operating conditions: 0 ~ 50℃, 0 ~ 95% ph/EC sensor Measurement and control of ph/EC for microalgae growth Measuring range: 0 ~ 9.99dS/m
Measurement error: ±0.5 video
image Network, API, and multiple access support CCTV are used to acquire image information of a web application and a control system to monitor the culture progress or collect data. Analysis between information on the capture screen by period rather than real-time video information VGA-class support
Wired and wireless communication support
Motion capture function

Such culture conditions include, for example, a process of primary culturing microalgae inoculated with seed germs in the first culture space (2111) to the 6th culture space (2116), a secondary culture process of algebraically proliferating microalgae, and microalgae It can be divided into three stages of the tertiary cultivation process to produce an active substance that is a secondary metabolite, and can be set differently for each stage. As another example, the cultivation process of step 3 is divided into 6 processes in more detail, and the culture conditions at this time are for 6 culture chambers 2100 of the first culture space 2111 to the 6th culture space 2116. All may be set differently, and in this case, the setting conditions may be set to increase or increase by about 5 to 20% sequentially.

On the other hand, during the process of culturing microalgae inoculated with the seed, the process of algebraic proliferation of cells, and the process of generating active substances, the first culture space 2111 provides an increasingly wider receiving area. As it goes to the 6th culture space 2116, the floor surface area of 5 to 20% may be gradually increased. In addition, the lighting conditions, including the illuminance of each culture chamber 2100, may increase by 5-20% from the first culture space 2111 to the sixth culture space 2116, and the number of LED installations is also 5 ~ 20% more and more can be installed.

<Cultivation method and operation>

Hereinafter, the culture method and operation of the microalgae culture apparatus during continuous flow control according to a preferred embodiment of the present invention will be described with reference to FIGS. 6 to 10. Here, the lifting bulkhead 2121 will be described as an example. In addition, the culture chamber 2100 is provided with a first culture space (2111) to a sixth culture space (2116) to refine the three-step process of the microalgae to obtain the three-step active material in six processes, at this time The microalgae culture, migration, and operation of the culture device will be described.

First, the microalgae inoculated with the seed bacteria are supplied to the first culture space 2111 (S10). As shown in FIG. 7, microalgae accommodated in a separate supply tank can be supplied to the first culture space 2111. The microalgae at this time may be microalgae prior to the culture in which the seed bacteria were inoculated. In addition, the first culture space 2111 to the sixth culture space 2116 may be blocked by all of the first ascent partition wall 2121 to the sixth ascent partition wall 2121, and may be disposed substantially horizontally as an initial position. In addition, the microalgae of the first culture space 2111 may be cultured for a period of time in the first culture space 2111 provided with the optimum culture conditions corresponding to the control.

Next, when the culture is completed, as shown in FIGS. 8 and 9, the microalgae of the first culture space 2111 is moved to the second culture space 2112 (S20). The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. When the culture is completed (S21) by monitoring of the control unit, the culture chamber 2100 may be axially rotated around the rotation shaft 1100 by the lifting means 3000 to be positioned obliquely. Thereafter, the first elevating partition 2121 may be raised, and the microalgae of the first culture space 2111 may be moved to the second culture space 2112 by pushing the microalgae with the moving member 2122 (S22). Therefore, the microalgae can be moved by gravity from the first culture space 2111 to the second culture space 2112.

When the movement is completed, check whether the first culture space 2111 is empty (S23), lower the first elevating partition 2121 to block the second culture space 2112, and return the culture chamber 2100 to its original position. By rotating the shaft, it is possible to supply microalgae inoculated with seed bacteria to the first culture space 2111 (S10).

Next, the microalgae are cultured in the first culture space 2111 and the second culture space 2112, and when the culture is completed, the microalgae of the second culture space 2112 are moved to the third culture space 2113. It can be made (S30). The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. When the culture is completed (S31), the culture chamber 2100 can be axially rotated by the lifting means 3000 to be inclined. Thereafter, the second elevating partition 2121 may be raised, and the microalgae of the second culture space 2112 may be first moved to the third culture space 2113 by pushing the microalgae with the moving member 2122 (S32). ). Therefore, the microalgae may be moved by gravity from the second culture space 2112 to the third culture space 2113.

When the first movement is completed, check whether the second culture space 2112 is empty (S33), lower the second elevation barrier 2121 to block the third culture space 2113, and the first elevation barrier 2121 By raising the, and pushing the microalgae to the moving member 2122, the microalgae of the first culture space 2111 can be moved to the second culture space 2112 secondary (S22).

In addition, when the second movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and the first elevation barrier 2121 may be lowered to block the second culture space 2112. The culture chamber 2100 can be axially rotated to the original position, and microalgae inoculated with seed germs can be supplied to the first culture space 2111 (S10).

Next, the microalgae are cultured in the first culture space 2111 to the third culture space 2113, and when the culture is completed, the microalgae of the third culture space 2113 are moved to the fourth culture space 2114. Let (S40). The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. When the culture is completed (S41), the culture chamber 2100 by the lifting means 3000 may be positioned to be inclined while rotating. Thereafter, the third elevation partition 2121 may be raised, and the microalgae of the third culture space 2113 may be first moved to the fourth culture space 2114 by pushing the microalgae with the moving member 2122 (S42). ). Therefore, the microalgae can move from the third culture space 2113 to the fourth culture space 2114 by gravity.

When the primary movement is completed, check whether the third culture space 2113 is empty (S43), and lower the third elevation barrier 2121 to block the fourth culture space 2114, and the second elevation barrier 2121 ) And push the microalgae to the moving member 2122 to move the microalgae of the second culture space 2112 to the third culture space 2113 (S32).

In addition, when the second movement is completed, confirms whether the second culture space 2112 is empty (S33), lowers the second lift barrier 2121 to block the third culture space 2113, and the first lift barrier The microalgae of the first culture space 2111 may be moved to the second culture space 2112 for a third time by raising the 2121 and pushing the microalgae to the moving member 2122 (S22).

Then, when the third movement is completed, it is confirmed that the first culture space 2111 is empty (S23), and the first elevating partition 2121 is lowered to block the second culture space 2112. The culture chamber 2100 can be axially rotated to the original position, and microalgae inoculated with seed germs can be supplied to the first culture space 2111 (S10).

Next, the microalgae are cultured in the first culture space 2111 to the fourth culture space 2114, and when the culture is completed, the microalgae of the fourth culture space 2114 are moved to the fifth culture space 2115. Let (S50). The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. When the culture is completed (S51), the culture chamber 2100 by the lifting means 3000 may be positioned to be inclined while rotating. Thereafter, the fourth altitude partition wall 2121 is raised and the microalgae are moved to the moving member 2122 to primarily move the microalgae of the fourth culture space 2114 to the fifth culture space 2115 (S52). . Therefore, the microalgae can move from the fourth culture space 2114 to the fifth culture space 2115 by gravity.

In addition, when the first movement is completed, check whether the fourth culture space 2114 is empty (S53), and lower the fourth elevation barrier 2121 to block the fifth culture space 2115, and the third elevation barrier The microalgae of the third culture space 2113 may be secondarily moved to the fourth culture space 2114 by raising the 2121 and pushing the microalgae to the moving member 2122 (S42).

In addition, when the second movement is completed, check whether the third culture space 2113 is empty (S43), and lower the third elevation barrier 2121 to block the fourth culture space 2114, and the second elevation barrier (2121) The microalgae of the second culture space 2112 may be moved to the third culture space 2113 by pushing the microalgae to the moving member 2122 (S32).

In addition, when the third movement is completed, check whether the second culture space 2112 is empty (S33), lower the second lift barrier 2121 to block the third culture space 2113, and the first lift barrier (2121) by raising and pushing the microalgae to the moving member 2122, the microalgae of the first culture space 2111 can be moved to the second culture space 2112 four times (S22).

Then, when the fourth movement is completed, it is confirmed that the first culture space 2111 is empty (S23), and the first elevating partition 2121 is lowered to block the second culture space 2112. The culture chamber 2100 can be axially rotated to the original position, and microalgae inoculated with seed germs can be supplied to the first culture space 2111 (S10).

Next, the microalgae are cultured in the first culture space 2111 to the fifth culture space 2115, and when the culture is completed, the microalgae of the fifth culture space 2115 are moved to the sixth culture space 2116. Let (S60). The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. When the culture is completed (S61), the culture chamber 2100 by the lifting means 3000 may be positioned to be inclined while rotating. Thereafter, the fifth elevating partition 2121 may be raised and the microalgae of the fifth culture space 2115 may be first moved to the sixth culture space 2116 by pushing the fine member to the moving member 2122 (S62). Accordingly, the microalgae can move from the fifth culture space 2115 to the sixth culture space by gravity.

When the primary movement is completed, check whether the fifth culture space 2115 is empty (S63), lower the fifth elevation barrier 2121 to block the sixth culture space 2116, and block the fourth elevation barrier 2121 ) And push the microalgae to the moving member 2122 to move the microalgae of the fourth culture space 2114 to the fifth culture space 2115 (S52).

In addition, when the second movement is completed, check whether the fourth culture space 2114 is empty (S53), and descend the fourth elevation barrier 2121 to block the fifth culture space 2115, and the third elevation barrier By raising the (2121) and pushing the microalgae to the moving member 2122, the microalgae of the third culture space 2113 can be moved to the fourth culture space 2114 for a third time (S42).

In addition, when the third movement is completed, the third culture chamber 2100 is checked to see if it is empty (S43), the third lifting barrier 2121 is lowered to block the fourth culture space 2114, and the second lifting barrier ( 2121) and the microalgae of the second culture space 2112 can be moved to the third culture space 2113 by pushing the microalgae to the moving member 2122 (S32).

In addition, when the fourth movement is completed, confirms whether the second culture space 2112 is empty (S33), lowers the second lift barrier 2121 to block the third culture space 2113, and the first lift barrier (2121) by raising and pushing the microalgae to the moving member 2122, the microalgae of the first culture space 2111 can be moved to the second culture space 2112 four times (S22).

Then, when the fifth movement is completed, it is confirmed that the first culture space 2111 is empty (S23), and the first elevating partition 2121 is lowered to block the second culture space 2112. The culture chamber 2100 can be axially rotated to the original position, and microalgae inoculated with seed germs can be supplied to the first culture space 2111 (S10).

Finally, the microalgae are cultured in the first culture space 2111 to the sixth culture space 2116, and when the culture is completed, the microalgae are discharged and harvested from the sixth culture space 2116 as shown in FIG. (S70), and the microalgae from the fifth culture space (2115) to the sixth culture space (2116), the third culture space (2113) to the fourth culture space (2114), the second culture space (2112) To the third culture space 2113, the first culture space 2111 may be moved to the second culture space, and microalgae inoculated with seed germs may be supplied to the first culture space 2111. The microalgae of each culture chamber 2100 can be cultured for a period of time under a corresponding optimal culture condition. In addition, when the culture is completed in each culture chamber (2100) (S71), the culture chamber 2100 by the lifting means 3000 may be positioned to be inclined while rotating. Subsequently, the 6th elevation partition 2121 is raised to discharge the microalgae of the 6th culture space 2116 to harvest (S72 ). Therefore, the microalgae can be discharged by gravity from the sixth culture space 2116 to the outside.

When the harvesting is completed, check whether the sixth culture space 2116 is empty (S73), lower the sixth elevation barrier 2121 to block the outside and the sixth culture space 2116, and the fifth elevation barrier 2121 ) And push the non-manufactured by the moving member 2122 to first move the microalgae of the fifth culture space 2115 to the sixth culture space 2116 (S62).

In addition, when the first movement is completed, check whether the fifth culture space 2115 is empty (S63), lower the lifting bulkhead 2121 to block the sixth culture space 2116, and the fourth lifting bulkhead ( 2121) and the microalgae of the fourth culture space 2114 can be moved to the fifth culture space 2115 by pushing the microalgae to the moving member 2122 (S52).

In addition, when the second movement is completed, check whether the fourth culture space 2114 is empty (S53), and descend the fourth elevation barrier 2121 to block the fifth culture space 2115, and the third elevation barrier By raising the (2121) and pushing the microalgae to the moving member 2122, the microalgae of the third culture space 2113 can be moved to the fourth culture space 2114 for a third time (S42).

In addition, when the third movement is completed, check whether the third culture space 2113 is empty (S43), and lower the third elevation barrier 2121 to block the fourth culture space 2114, and the second elevation barrier By raising the (2121) and pushing the microalgae to the moving member 2122, the microalgae of the second culture space 2112 can be moved to the third culture space 2113 four times (S32).

In addition, when the fourth movement is completed, confirms whether the second culture space 2112 is empty (S33), lowers the second lift barrier 2121 to block the third culture space 2113, and the first lift barrier (2121) by raising and pushing the microalgae to the moving member 2122, the microalgae of the first culture space 2111 can be moved to the second culture space 2112 four times (S22).

Then, when the fifth movement is completed, it is confirmed that the first culture space 2111 is empty (S23), and the first elevating partition 2121 is lowered to block the second culture space 2112. The culture chamber 2100 can be axially rotated to the original position, and microalgae inoculated with seed germs can be supplied to the first culture space 2111 (S10).

On the other hand, to complete the cultivation and harvesting of the microalgae, the microalgae inoculated with the seed are no longer supplied to the first culture space 2111, and the microalgae of the previous culture chamber 2100 are transferred to the next culture chamber 2100. Transfer, and repeat the process of harvesting through the last culture chamber 2100.

In addition, the bottom surface area of the first culture space 2111 may be the narrowest and gradually widened, and then the bottom surface area of the sixth culture space 2116 may be the largest.

In addition, in the above-described processes (S10 to S70), the culture conditions of the culture chamber 2100, the determination of the completion of the culture, the operation of the lifting means 3000, the first lifting bulkhead 2121 to the sixth lifting bulkhead 2121 Operation and judgment of all components, including operation, etc., can be made at the control unit. In addition, when the culture time of each culture chamber 2100 is set equally, and the completion of the subsequent culture is confirmed, it can be determined that the culture of the vehicle is also completed, and the next process can be performed. In addition, regardless of the cultivation time, the next process may be carried out only after the subsequent cultivation and the cultivation of the entire vehicle are completed.

On the other hand, the above-described process may be implemented to operate the operator wirelessly through a smartphone, tablet, laptop or PC. Briefly, various information including light source, illuminance, temperature, humidity and carbon dioxide are collected and monitored by various sensors in each process cultivation, and the collected information is analyzed on a computer in real time and controlled by a control device. It can be installed to adjust the growth conditions and environment of microalgae.

In addition, the monitoring and control system can be monitored and managed by a smartphone through web-based software, and an alarm function by text (including SMS) transmission can be configured when an abnormality occurs. At this time, the control system may be configured such that the function of sending an alert when the abnormality is less than or exceeding the value set for each sensor is extended.

In addition, sensing and control can be configured through embedded systems such as light sources, cell growth, temperature, humidity, carbon dioxide, illuminance, wavelength, and video images for specialized control of microalgae for the production of bioactive materials. At this time, the output of the sensor to be used is an analog and digital output, which is integrated through a multi-channel DAC board and controls the light source, cell growth, temperature, humidity, carbon dioxide, illuminance, wavelength, etc. according to the information to realize an optimized culture environment. Can. In addition to these conditions, LED designs and modules can be manufactured to install a composite structure LED module in which LEDs for each wavelength are combined in order to control wavelength and illuminance optimized for microalgae growth for each process. In addition, the heat generated from the LED can be configured in a way to control in conjunction with cooling water for temperature control to save energy.

As described above, those skilled in the art to which the present invention pertains will understand that the present invention may be implemented in other specific forms without changing its technical spirit or essential features. Therefore, the above-described embodiments are to be understood in all respects as illustrative and not restrictive. The scope of the present invention is indicated by the claims of the patent registration to be described later rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present invention. do.

1000: base
1100: rotating shaft
2000: Culture Department
2100: culture chamber 2110: culture space
2111: 1st culture space 2112: 2nd culture space
2113: 3rd culture space 2114: 4th culture space
2115: 5th culture space 2116: 6th culture space
2120: bulkhead means 2121: lifting bulkhead
2122: moving member 2123: moving bulkhead
2124: magnetic material 2125: iron plate
2200: Lighting means
2210: 1st light source 2220: 6th light source.
3000: means of elevating.

Claims (7)

In a continuous culture device designed to cultivate microalgae,
It includes; a partition wall means (2120) and a culture unit (2000) having a culture chamber (2100) in which the culture space (2110) is divided by the culture process of the microalgae by the partition means (2120);
The partition wall means 2120 is installed to move the microalgae of the culture space 2110 to the next culture space 2110 by raising and lowering the partition wall 2121 to open and close neighboring culture spaces 2110, or move Flow control type continuous microalgae culture device, characterized in that installed to move the partition wall 213 to move the microalgae to the position of the next culture space in the culture space.
In claim 1,
The culture space (2110) is a flow control type continuous microalgae culture device characterized in that the bottom surface area is different for each culture process.
In claim 1 or 2,
The partition wall means (2120) is arranged to be adjacent to the lifting partition (2121) installed to elevate while being divided into a plurality of culture spaces (2110), and push the microalgae of the culture space (2110) to the next culture space (2110). Flow control type continuous microalgae culture device further comprising a moving member (2122) installed.
In claim 3,
When the culture chamber 2100 is seated, the base 1000 is installed such that one side of the culture chamber 2100 is rotated axially; and
Further comprising; lifting means (3000) installed to axially rotate the culture chamber 2100;
Flow control type continuous microalgae culture apparatus characterized in that the microalgae of the culture space (2110) moves to the next culture space by gravity.
In paragraph 1 or 2
The partition wall means (2120) further includes a magnetic body (2124) installed to move the moving partition wall (213) arranged to be divided into a plurality of culture spaces (2110) by magnetic force,
The moving bulkhead 213 and the magnetic body 2124 is pushed to move the microalgae for each culture process, and when the microalgae are discharged, the flow control type continuous microalgae is characterized in that it is installed to move to partition the initial culture space 2110. Culture device.
In a method of culturing microalgae step by step in multiple culture spaces,
A supply step of supplying the microalgae inoculated with the seed bacteria to the initial culture space 2110 provided in the culture chamber 2100 by the partition wall means 2120;
A moving and supplying step of moving the microalgae of the first culture space 2110 to the next culture space 2110, and supplying microalgae inoculated with seed bacteria to the first culture space 2110;
A repeating step of repeating the moving and feeding step; and
The discharge step of discharging the cultured microalgae from the end of the culture space 2110;
The partition wall means 2120 sequentially raises and lowers the partition wall 2121 so that the neighboring culture space 2110 sequentially passes, and moves the moving member 2122 reciprocally to move the microalgae of the culture space 2110 next. A method of culturing a flow-controlled continuous microalgae, characterized in that the microalgae are moved to the next culture space 2110 while moving to the culture space 2110 or the moving bulkheads 213 are moved.
In claim 6,
The culture chamber 2100 is inclined, and the lifting bulkhead 2121 is raised to move microalgae from the culture space 2110 to the next culture space 2110 by gravity, or
The moving bulkhead 213 is in contact with the bottom of the culture chamber 2100 by the magnetic material 2124 to move or slide the cloud,
Characterized in that the method of culturing a flow-controlled continuous microalgae.

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