KR102193850B1 - Flow Control type continuous microalgae cultivating device and cultivating method thereof - Google Patents

Abstract

The present invention relates to a flow control type continuous microalgae cultivation apparatus, in a continuous culture apparatus manufactured to cultivate microalgae, a partition wall means, and a culture chamber in which culture spaces are divided by the culture process of the microalgae by the partition means. By having a culture unit having; By having, when the culture is completed step by step, the microalgae are moved from the culture space to the next culture space by the barrier means, and the next stage of culture is provided, and the culture step is subdivided according to the microalgae It is to be able to provide n number of culture spaces.

Description

Flow Control type continuous microalgae cultivating device and cultivating method thereof

The present invention relates to a continuous microalgal culture apparatus, and more specifically, the culture chambers are divided into open and close partitions for each culture step, and each culture chamber is opened sequentially after the culture chambers are tilted for each culture step. It relates to a flow-controlled continuous microalgae culture apparatus and a culture method in which the microalgae contained in the cell flow to the next culture chamber.

In general, microalgae are the oldest organisms on the planet, and the number of them reaches several thousand species, but about 0.1% of them are known for physiological activity, and only a few are cultivated on an industrial scale, of which chlorella or spirulina. Microalgae such as (spirulina) are being developed as a variety of materials such as dietary supplements, health supplements, aquaculture feed, alternative medicines and energy resources.

On the other hand, physiologically active substances produced in plants are secondary metabolites, and are known as substances that produce antioxidant, anti-inflammatory, antifungal, antibacterial and anti-cancer properties to protect themselves from the outside after cell growth is completed. Carotenoids produced from plants are also representative secondary metabolites, and cases produced from microalgae have been reported. In addition, it is known that the production of physiologically active substances produced in microalgae is not produced during the logarithmic growth period of the strain, but in the resting period in which growth is stopped, so for the maximum production of physiologically active substances, the primary culture and the maximizing cell culture in the logarithmic period, It is essential to construct a two-stage growth of secondary culture that maximizes the production of bioactive substances using the maximum number of cells during the resting period. Accordingly, the culture conditions for seed inoculation are greatly affected by the osmotic pressure and shear stress, so that the culture conditions that minimize the impact on the cells must be provided, and in consideration of this, the development of a continuous flow culture device is actively progressing.

Briefly describing such a conventional culture apparatus, as shown in FIG. 1, the lower end of the first vertical pipe 10 to supply microalgae to the plurality of first vertical pipes 10 and the plurality of first vertical pipes 10 A second vertical pipe 30 installed in the first horizontal pipe 20, a plurality of second vertical pipes 30 disposed adjacent to the first vertical pipe 10, and a plurality of second vertical pipes 30 ), and a second horizontal pipe 40 installed at the bottom of each other, and a connection pipe 50 installed by connecting any one of the first vertical pipes 10 and the second vertical pipes 30 adjacent to each other. Therefore, the microalgae supplied from the first horizontal pipe 20 are supplied to the first vertical pipe 10, and the microalgae cultivated in the first vertical pipe 10 are transferred to the second horizontal pipe through the connection pipe 50. It may be supplied to 40 and the second vertical pipe (30). Thereafter, when the culture is completed, it can be discharged through any one of the second vertical pipes 30.

However, in order to supply the initial microalgae to the first horizontal pipe 10, to move the microalgae from the first vertical pipe 10 to the second vertical pipe 20, or to discharge the microalgae that has been cultured Since a transfer device including a pump is required, there is a problem that the production and maintenance cost of the culture device is largely required.

In addition, there is another problem in that the quality of the final product is lowered because the degree of cultivation of microalgae in each vertical tube is disregarded, and the quality of the final product is lowered and mass production through real-time control is difficult.

Korean Patent Registration No. 10-1721448 (announced on March 30, 2017.) Korean Patent Registration No. 10-1567140 (announcement on 09.11.015) Korean Patent Registration No. 10-0585216 (20006.06.08. Announcement) Republic of Korea Patent Publication No. 10-2013-0063276 (published on June 14, 2013)

It is an object of the present invention conceived to solve the above-described problem is that a plurality of culture chambers in which the surface area of the bottom increases according to the cultivation step of microalgae are sequentially arranged, and when the partition walls are sequentially opened as the culture chambers are inclined, Thus, by moving the microalgae being cultured in the culture chamber to the next stage of the culture chamber, it is intended to provide a flow-controlled continuous microalgal culture apparatus and a culture method manufactured to enable mass production through step-by-step culture.

In order to achieve the above object, the flow-controlled continuous microalgae culture apparatus according to the present invention is a continuous culture apparatus manufactured to cultivate microalgae, a barrier rib means, and the culture spaces by the barrier rib means a process of culturing microalgae. It may include; a culture unit having a culture chamber divided into.

At this time, the bulkhead means is installed to open and close neighboring culture spaces by raising and lowering the lifting bulkhead to move the microalgae in the culture space to the next culture space, or by pushing the microalgae while moving the moving partition wall to cultivate the next culture in the culture space. It can be installed to move to a location in space.

In addition, the culture spaces may have different bottom surface areas for each culture process.

In addition, the partition wall means is disposed to be adjacent to the lifting partition wall installed to lift while being divided into a plurality of culture spaces, and may further include a moving member installed to push the microalgae in the culture space to the next culture space.

In addition, the base is installed so that one side of the culture chamber axially rotates while the culture chamber is seated; and an elevating means installed to axially rotate the culture chamber in an oblique direction. At this time, the microalgae in the culture space may move to the next culture space by gravity.

The partition wall means may further include a magnetic body installed to move the moving partition wall arranged to be divided into a plurality of culture spaces by magnetic force.

At this time, the movable partition wall and the magnetic body may be installed to move the microalgae for each culture process by pushing the microalgae, and when discharging the microalgae, they move to partition the initial culture space.

On the other hand, the flow-controlled continuous microalgae culture method according to the present invention is a method of culturing microalgae in a plurality of culture spaces step by step, and the microalgae inoculated with the seed are placed in the first culture space provided in the culture chamber by the partition means. A supply step of supplying; A moving and supplying step of moving the microalgae in the first culture space to the next culture space and supplying the microalgae inoculated with the seed in the first culture space; It may include a repeating step of repeating the moving and supplying step; And a discharge step of discharging the cultured microalgae from the final culture space.

At this time, the bulkhead means sequentially raises the lifting bulkhead to allow the neighboring culture spaces to pass in sequence, and moves the microalgae in the culture space to the next culture space by reciprocating the moving member, or the microalgae as the moving bulkheads move. Can be moved to the next culture space.

In addition, by tilting the culture chamber and raising the lifting partition wall, the microalgae can move from the culture space to the next culture space by gravity.

In addition, the movable bulkhead can be rolled or slided by contacting the bottom of the culture chamber by a magnetic substance.

As described above, according to the present invention, by providing at least three, for example, six culture spaces of inoculation initial growth, logarithmic growth phase, and secondary metabolite production, and by installing lighting means for each culture space, it is optimal for each culture space. There is an effect that can provide illuminance, wavelength and temperature.

In addition, the lighting means is equipped with an LED module of a complex structure in which LEDs for each wavelength are combined, so that the optimum wavelength is provided for each culture process, and a number of sensors are installed in each culture space to provide light sources and cells required for microalgae growth. There is an effect of measuring growth, temperature, humidity, carbon dioxide, illuminance, wavelength, etc. to maintain an optimal state at all times.

In addition, when the culture is completed step by step, the microalgae are moved from the culture space to the next culture space by the barrier means to provide the next stage of culture, and the culture stage is subdivided according to the microalgae to provide n culture spaces. There is an effect it can provide.

In addition, in the case of the moving partition wall and the magnetic material, the size of the bottom surface area of the culture space can be arbitrarily changed, thereby maximizing the amount of production.

The following drawings appended in the present specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the detailed description of the present invention, so the present invention is limited to the matters described in such drawings. And should not be interpreted.
1 is a schematic diagram of a conventional culture apparatus.
2 is a side view schematically showing a flow control type continuous microalgal culture apparatus according to a preferred embodiment of the present invention.
3 is a perspective view illustrating the culture chamber shown in FIG. 2.
Figure 4 is a side view showing an operating state of the culture apparatus shown in Figure 2;
5A to 5C are side views schematically illustrating a flow-controlled continuous microalgal culture apparatus according to another embodiment.
6 is a block diagram showing a method of culturing microalgae using the culture apparatus of FIG. 2.
7 to 10 are diagrams schematically showing a reduction in the movement process of microalgae in the culture method shown in FIG. 6.

Hereinafter, exemplary embodiments will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present invention. However, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

<Configuration>

In the flow-controlled continuous microalgal culture apparatus according to the present invention, a plurality of partition walls are arranged at regular intervals in one culture chamber to provide a plurality of culture spaces, and microalgae can be grown in each culture space in each culture step. As an example, the partition wall means may include a plate-shaped lifting partition wall and a plate-shaped moving member reciprocating in the left and right directions. In addition, as another example, the partition wall means may include a moving partition having a cylindrical or inverted "T" shape and moving or sliding, and a magnetic material that moves the moving partition by magnetic force.

When the step-by-step growth is completed, the culture chamber may be tilted to move the microalgae from the culture chamber of one culture stage to the culture chamber of the next culture stage by reciprocating movement of the moving member along with gravity. At this time, the culture chamber is provided with at least 4 culture spaces to cultivate microalgae inoculated with the seed, to proliferate the microalgae, and to induce the proliferated microalgae to a resting period to generate physiologically active substances, which are secondary metabolites. Can be.

In addition, briefly describing the cultivation process and method of an embodiment, the microalgae inoculated with the seed may be supplied to the primary culture space to be primary cultured for a certain period of time. When the culture chamber is inclined after the first culture, the lifting bulkhead is opened, and the first cultured microalgae can be moved from the first culture space to the second culture space while the moving member reciprocates. Thereafter, the elevating partition wall is closed, the culture chamber is returned to the horizontal level, and then the microalgae inoculated with the seed can be supplied to the primary culture space. After repeating this operation or process, the microalgae are moved to the final culture space, and when the culture is finished, the culture chamber is tilted, and when the lifting partition is opened, the movable member reciprocates and the fully cultured microalgae can be harvested. Of course, the elevating partition is closed again, the operation and process of transferring the microalgae from the previous culture chamber to the next culture chamber are repeated, and the microalgae inoculated with the seed are supplied to the first culture space, thereby continuously culturing and Can be harvested. These operations or processes can be repeated to cultivate and harvest microalgae in large quantities.

Briefly explaining the culture process and method of another embodiment, a plurality of moving partitions and magnetic bodies are spaced apart from each other in the culture chamber to provide culture spaces, and microalgae inoculated with the seed are first supplied to the culture space for a certain period of time. Can be cultured. After the secondary culture, the movable partition walls and magnetic bodies move, so that the microalgae can be moved to the secondary culture space. At this time, another moving partition wall and a magnetic material are disposed inside the culture chamber to provide a primary culture space, and microalgae inoculated with the seed can be supplied to the primary culture space. These operations and processes are repeated, and when the last culture is completed, the moving partition wall and the magnetic body move, and microalgae can be discharged to the outside of the culture chamber. By repeating this process, microalgae can be cultured and harvested in large quantities.

In addition, since cells proliferate when microalgae are cultured in the culture space of each step, the culture space of the next culture step may have a bottom surface area of about 5 to 20% wider than that of the culture space of the previous culture step. In addition, for each culture space, various culture conditions including light source, illuminance or amount of illumination, temperature, carbon dioxide, acidity and electrical conductivity (ph/Ec), etc., can be adjusted to an optimal state and supplied according to the culture stage.

<Example>

The flow control type continuous microalgal culture apparatus according to a preferred embodiment of the present invention includes a base 1000, a culture unit 2000, and an elevating means 3000, as shown in FIGS. 2 and 4.

First, in the base 1000, a culture unit 2000 is mounted, and an elevating means 3000 for elevating the culture unit 2000 is installed. At this time, the base 1000 may be installed such that one portion of the culture unit 2000 is coupled to the rotation shaft 1100, and the culture unit 2000 can axially rotate around the rotation axis 1100.

Meanwhile, the culture unit 2000 may be configured to generate a physiologically active substance as a secondary metabolite through the growth stage of the logarithmic growth phase of the microalgae inoculated with the seed. The culture unit 2000 may include a culture chamber 2100 and a lighting means 2200.

Here, as shown in FIGS. 2 and 3, the culture chamber 2100 is manufactured to accommodate and culture microalgae in each culture step. The culture chamber 2100 may be in the form of a tank having a substantially long length. One side of the discharge unit may be installed to open and close the culture space 2110 by rotating the upper portion of the microalgae to discharge the microalgae to the outside. This culture chamber 2100 may be provided with a plurality of culture spaces 2110 and partition means 2120.

The culture space 2110 may be provided in plural to accommodate microalgae in each culture step. These culture spaces 2110 are for three stages of culture, such as the primary culture of microalgae inoculated with the seed, the secondary culture of proliferating microalgae, and the tertiary culture of producing physiologically active substances, which are secondary metabolites. At least four or more may be provided. As an example, the culture space 2110 is the first culture space 2111 in which microalgae are first cultured, as shown in FIGS. 2 and 4, 6 of the sixth culture space 2116 in which microalgae are finally cultured. I can. This culture space 2110 may be provided as needed.

In addition, in the culture spaces 2110, as the first culture, the second culture, and the third culture of microalgae are in progress, the bottom surface area becomes wider, and this may be adjusted according to the installation position of the partition means 2120. For example, as the first culture space 2111 goes to the sixth culture space 2116, the floor surface area may increase by about 5 to 20%. This is to prepare conditions for more cells to proliferate as they become cultured. In addition, the bottom surface area may be different for each culture space 2110, and may be different for each set by several.

The partition wall means 2120 may include an elevation partition wall 2121 and a moving member 2122.

The elevating partition wall 2121 may be provided in plural to divide the culture chamber 2100 into three or more or six to provide a plurality of culture spaces 2110. The lifting partition wall 2121 may be installed to lift along the guide groove on the inner surface of the culture chamber 2100 to block or open the adjacent culture chambers 2100. Accordingly, when the lifting partition walls 2121 descend to block the respective culture spaces 2110, the flow of microalgae in each culture space 2110 is blocked, so that culture can proceed in each culture space 2110. In addition, when the elevating partition walls 2121 rise sequentially and each culture space 2110 is opened sequentially, microalgae may move from the culture space 2110 of the previous culture step to the culture space 2110 of the next culture step. At this time, the culture spaces 2110 may be in an inclined state together with the culture chamber 2100 inclined by the lifting means 3000 so that the microalgae are moved by gravity. In addition, while the neighboring previous culture space 2110 and the next culture space 2110 are open, the moving member 2122 moves to the next culture chamber 2100 to completely culture the microalgae in the previous culture chamber 2100. When the chamber 2100 is pushed out, the lifting partition wall 2121 may be lowered to block the previous culture space 2110 and the next culture space 2110. The elevating bulkhead 2121 may include, for example, a first lifting bulkhead 2121 to a sixth lifting bulkhead 2121 as shown in FIG. 2. In addition, the lifting partition wall 2121 may be installed to descend to open the culture chamber 2100 and ascend to close the culture chamber 2100.

The moving member 2122 may be installed to forcibly move the microalgae that may remain in the previous culture space 2110 according to the inclination angle of the culture chamber 2100 to move to the next culture space 2110. The moving member 2122 may be installed so as to reciprocate from one side elevation partition wall 2121 to the other side elevation partition wall 2121. For example, it may be installed to be in contact with the one side lifting partition wall 2121 and to return after moving to the other side lifting partition wall 2121. The moving member 2122 may have a plate shape, and the lower portion may be bent or a separate member may be attached to the lower portion to facilitate movement while sweeping the bottom of the culture chamber 2100. In addition, the moving member 2122 may be installed every six culture spaces 2110, for example, and may be installed every five culture spaces 2110 except the last culture space 2110.

In addition, the lighting means 2200 may be installed to provide lighting in each culture space 2110 according to a set condition including an appropriate illuminance and an amount of illumination for cultivation of microalgae. These lighting means 2200 are installed on the upper surface and/or side and/or lower surface of each culture space 2110, and may include a light source including an LED. For example, the lighting means 2200 may include first light sources 2210 to 6th light sources 2220 installed in each of the first culture space 2111 to the sixth culture space 2116. The first to sixth light sources 2210 to 2220 may provide different optimal lighting conditions according to the cultivation step of each culture space 2110. As an example, the number of LEDs may be further installed in the first light source 2210 to the sixth light source 2220 so that the illuminance is sequentially increased by approximately 5 to 20%. In addition, the first light source 2210 to the sixth light source 2220 may be installed on a panel disposed on the upper surface of the culture chamber 2100, installed on the side of the culture chamber 2100, or installed on the lower surface of the culture chamber. have.

In addition, the lighting means 2200 may include a composite LED module in which LEDs for each wavelength are combined to control the wavelength and illuminance optimized for microalgae growth for each process by manufacturing an LED design and module. In addition, the heat generated from the LED can be configured in a manner that is controlled in conjunction with cooling water for temperature control in order to save energy.

Meanwhile, the lifting means 3000 may be installed to rotate the culture chamber 2100 axially. As shown in FIGS. 2 and 4, the lifting means 3000 is one or two or more disposed under the culture chamber 2100 at regular intervals, and a culture chamber coupled to the base 1000 by a rotating shaft 1100 It can be installed to rotate the axis (2100). At this time, the inclination of the culture chamber 2100 is that the microalgae does not overflow while the entire amount of microalgae is pushed from the culture space 2110 to the next culture space 2110 by the opening of the lifting partition wall 2121 and the moving member 2122. It may not be enough. The elevating means 3000 may be a variety of general devices including a cylinder and a cylinder rod, and may be installed at the lower, upper or side of the culture chamber 2100.

On the other hand, the culture apparatus according to the present invention may further include a plurality of sensors installed to measure various culture conditions in each culture space (2110). The outputs of these sensors are analog and digital outputs, which are integrated through a multi-channel DAC board, and according to the information, the light source, cell growth, temperature, humidity, carbon dioxide, illuminance, and wavelength can be controlled to realize an optimized culture environment. .

<Another embodiment>

Hereinafter, a flow-controlled continuous microalgal culture apparatus of the present invention according to another embodiment will be described in comparison with the embodiment of FIG. 2 with reference to FIGS. 5A to 5C.

5A and 5B, the culture unit 2000 includes a culture chamber 2100 in which the front and rear surfaces are opened and closed, and a partition wall means 2120 installed to provide a plurality of culture spaces 2110 in the culture chamber 2100. It may include.

The culture chamber 2100 may be installed such that one side of the sixth culture space 2116 side axially rotates in order to discharge the microalgae pushed by the partition wall means 2120 to the outside. In addition, the culture chamber 2100 may be installed so that the front side of the first culture space 2111 and the rear side of the sixth culture space 2116 rotate axially for movement of the partition means (2120) in a caterpillar form. have.

The partition means 2120 includes a moving partition 213 disposed in the culture chamber 2100 to divide the culture space 2110, and a magnetic material 2124 disposed on the outer surface of the culture chamber 2100 to move the moving partition 213. ) Can be provided.

Here, the moving partition wall 213 may have various shapes for dividing the culture space 2110, including a cylindrical shape, an inverted "T" shape, and a panel shape, and can be moved by the magnetic force of the magnetic body 2124 (鋼鐵) may be a material. At this time, the cylindrical shape reduces friction with the bottom of the culture chamber 2100 and facilitates rolling or sliding movement, and the inverted "T" shape increases the magnetic area with the magnetic body 2124 and reduces the weight of the culture chamber. It is possible to reduce the frictional resistance with the floor of (2100). In addition, the moving partition wall 213 and the magnetic body 2124 form a set, and may be installed as many as the number of culture spaces 2110. In addition, the moving partition wall 213 may be arranged to move together by magnetic force when the magnetic body 2124 moves. In addition, as shown in the drawing, the partition wall means 2120 may be installed to move in a caterpillar orbit, and for this purpose, the front and rear surfaces of the culture chamber 2100 may rotate flat and then return to their original position. At this time, the moving partition wall 213 may be installed to move while maintaining watertightness with the culture chamber 2100.

In addition, the magnetic body 2124 may be an electromagnet, and may be installed to move in a caterpillar shape separate from the moving partition wall 213. As shown in FIG. 5C, the magnetic body 2124 may be installed to be moved by the iron plate 2125 which is installed in a caterpillar shape at the bottom and moves.

In addition, the partition wall means 2120 may be installed to be moved so that the area of each of the culture spaces 2110 can be easily changed as needed. In addition, the moving partition wall 213 and the magnetic material 2124 may be installed to move in one direction according to the culture step. Therefore, when the cultivation step is completed at the corresponding position, the partition means 2120 pushes the microalgae to the next cultivation step position, and when the final cultivation is completed, the microalgae is pushed and discharged to the outside through the open rear side of the culture chamber 2100 Can be moved to For example, when the culture is completed and the partition wall means 2120 is moved by a certain distance, the first culture space 2111 may move to the position of the second culture space 2112 to become a second culture space 2112. have. As such, the partition wall means 2120 may be installed to move the microalgae together with the culture space to proceed with the next culture step, and to push the microalgae out of the culture chamber 2100 after the final culture step is completed. In addition, the moving partition wall 213 and the magnetic body 2124 from which the microalgae are discharged may be arranged to move in a caterpillar form to provide a first culture space 2111.

<cultivation conditions>

Hereinafter, culture conditions for culturing microalgae in a flow-controlled continuous microalgae culture apparatus according to a preferred embodiment of the present invention will be described. And, in the case of the figures presented below, there may be an error range of approximately 5 to 20% even if not indicated.

The culture apparatus according to the embodiment of the present invention may be manufactured to cultivate about 20L/day of microalgae. For example, when the receiving area of one culture chamber 2100 is 50 × 20 × 20 cm, microalgae having a height of about 10 cm may be cultured, and approximately 20 L of microalgae may be cultured. If the three chambers are continuous and the culture chamber 2100 is blocked by the lifting partition wall 2121 or the moving partition wall 213, the receiving area may be 50 × 80 × 20 cm. In addition, in the case of the culture chamber 2100 blocked by the lifting bulkhead 2121 or the moving bulkhead 213 in which six chambers are continuous, it may be 50 × 140 × 20cm, and nine chambers are continuous to the lifting bulkhead 2121 or In the case of the culture chamber 2100 blocked by the moving partition wall 213, it may be 50 × 2000 × 20 cm. Of course, the size of the culture chamber 2100 may vary depending on the amount of microalgae cultured.

In addition, the control unit measures the cell mass, temperature, cell mass, temperature, acidity (pH), carbon dioxide (CO ₂ ), illuminance, wavelength, etc. for each culture chamber 2100 in real time, and measures It can include four types of control functions that control carbon dioxide (CO ₂ ), temperature, illuminance, and acidity (pH) in real time by analyzing the value. As an example, the temperature is operated within the range of ±0.5 ℃ of the reference value suggested according to the culture conditions, the acidity (pH) is ±0.5 of the reference value, carbon dioxide (CO ₂ ) is ±0.5% of the reference value, and illuminance is standard It can be set to operate within ±2% of the value (lux). Various conditions including this are described in [Table 1] below.

division Development content Specifications Light source Optimized illumination and wavelength are the same according to step-by-step growth by using a method of mixing sunlight using an optical fiber integrated with sunlight or a lens, and an LED module suitable for growth. Sun wavelength: 2000 nm to 10000 nm
LED wavelength: 280 nm ~ 800 nm Illuminance In order to maintain proper illuminance, sunlight adjusts the optical filter, and in the case of LEDs, the applied current value is adjusted. Measuring range: 500 ∼10000 lux
Precision: ±0.5 Temperature In order to control the temperature according to the growth conditions, the efficiency of the system is increased by mixing the heat generated by the heat pad and the LED. Resolution: 0.1 ℃
Measuring range: -40 ℃ ~ + 65 ℃
Precision: ±0.5℃ Dioxide
carbon Carbon dioxide measurement and control for microalgae growth
Carbon dioxide measurement and control for microalgae growth Measuring range: 0 to 3000 ppm
Accuracy: ±0.5% ppm
(0 ~ 3000 ppm) @10 ~ 50℃
Operating conditions: 0 to 50℃, 0 to 95% ph/EC sensor PH/EC measurement and control for microalgae growth Measurement range: 0 ~ 9.99dS/m
Measurement error: ±0.5 video
image Network, API, and CCTV that can support multiple access are used to acquire image information of web applications and control systems to monitor culture progress or collect data. Analysis between the information of the captured screen by period rather than real-time video information VGA level support
Wired and wireless communication support
Motion capture function

Such culture conditions include, for example, a process of first culturing microalgae inoculated with a seed in the first culture space 2111 to 6th culture space 2116, a secondary culture process of logarithmic growth of microalgae, and microalgae. It is divided into three stages of the tertiary culture process that generates active substances, which are secondary metabolites, and can be set differently for each stage. As another example, the three-step culture process is divided into six processes in more detail, and the culture conditions at this time are for the six culture chambers 2100 of the first culture space 2111 to the sixth culture space 2116. All may be set differently, and in this case, the setting conditions may be set to rise or increase by about 5 to 20% sequentially.

On the other hand, in the first culture space 2111 to provide a wider receiving area during the process of culturing microalgae inoculated with the seed, the process of logarithmic growth in which cells proliferate, and the process of generating active substances. As it goes to the sixth culture space 2116, the floor surface area of 5 to 20% may become wider and wider. In addition, the lighting conditions including the illuminance of each culture chamber 2100 may increase by 5 to 20% from the first culture space 2111 to the sixth culture space 2116, and at this time, the number of LEDs installed is also 5 ~20% more and more can be installed.

<Cultivation method and operation>

Hereinafter, a method and operation of the flow-controlled continuous microalgae culture apparatus according to a preferred embodiment of the present invention will be described with reference to FIGS. 6 to 10. Here, the lifting partition wall 2121 will be described as an example. In addition, the culture chamber 2100 has a first culture space 2111 to a sixth culture space 2116 to subdivide the three-step process of microalgae to obtain the three-step active material into six processes. Microalgae culture and movement, and the operation of the culture device will be described.

First, the microalgae inoculated with the seed are supplied to the first culture space 2111 (S10). As shown in FIG. 7, microalgae accommodated in a separate supply tank may be supplied to the first culture space 2111. The microalgae at this time may be microalgae before the culture inoculated with the seed. In addition, the first culture space 2111 to the sixth culture space 2116 are all blocked by the first elevation partition wall 2121 to the sixth elevation partition wall 2121, and may be disposed substantially horizontally at the initial position. In addition, the microalgae in the first culture space 2111 may be cultured for a predetermined time in the first culture space 2111 provided with the corresponding optimal culture conditions by the control unit.

Next, when the culture is completed, as shown in FIGS. 8 and 9, the microalgae in the first culture space 2111 are moved to the second culture space 2112 (S20). The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. When the culture is completed (S21) by the monitoring of the control unit, the culture chamber 2100 may be rotated around the rotation shaft 1100 by the lifting means 3000 to be inclined. Thereafter, the first lifting partition wall 2121 is raised, and the microalgae in the first culture space 2111 can be moved to the second culture space 2112 by pushing the microalgae with the moving member 2122 (S22). Accordingly, the microalgae can be moved by gravity from the first culture space 2111 to the second culture space 2112.

When the movement is complete, check whether the first culture space 2111 is empty (S23), lower the first lifting bulkhead 2121 to block the second culture space 2112, and move the culture chamber 2100 to its original position. By rotating the axis, it is possible to supply the microalgae inoculated with the seed in the first culture space (2111) (S10).

Next, microalgae are cultured in the first culture space 2111 and the second culture space 2112, and when the culture is complete, the microalgae in the second culture space 2112 are moved to the third culture space 2113. Can be made (S30). The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. When the cultivation is completed (S31), the culture chamber 2100 may be rotated with the lifting means 3000 to be positioned at an angle. Thereafter, the second lifting partition wall 2121 may be raised, and the microalgae in the second culture space 2112 may be firstly moved to the third culture space 2113 by pushing the microalgae with the moving member 2122 (S32). ). Accordingly, the microalgae can be moved by gravity from the second culture space 2112 to the third culture space 2113.

When the first movement is completed, check whether the second culture space 2112 is empty (S33), lower the second elevation bulkhead 2121 to block the third culture space 2113, and the first elevation bulkhead 2121 And, by pushing the microalgae with the moving member 2122, the microalgae in the first culture space 2111 can be secondarily moved to the second culture space 2112 (S22).

In addition, when the second movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and lower the first elevation partition wall 2121 to block the second culture space 2112. The culture chamber 2100 is rotated to its original position, and the microalgae inoculated with the seed can be supplied to the first culture space 2111 (S10).

Next, microalgae are cultured in the first culture space 2111 to the third culture space 2113, and when the culture is complete, the microalgae in the third culture space 2113 are moved to the fourth culture space 2114. Make it (S40). The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. When the culture is completed (S41), the culture chamber 2100 may be positioned to be inclined while rotating by the lifting means 3000. Thereafter, the third lifting partition wall 2121 is raised, and the microalgae in the third culture space 2113 may be firstly moved to the fourth culture space 2114 by pushing the microalgae with the moving member 2122 (S42). ). Accordingly, the microalgae can move from the third culture space 2113 to the fourth culture space 2114 by gravity.

When the first movement is completed, it is checked whether the third culture space 2113 is empty (S43), the third elevation bulkhead 2121 is lowered to block the fourth culture space 2114, and the second elevation bulkhead 2121 ) And pushing the microalgae with the moving member 2122 to move the microalgae in the second culture space 2112 to the third culture space 2113 (S32).

In addition, when the second movement is completed, it is checked whether the second culture space 2112 is empty (S33), the second elevation partition wall 2121 is lowered to block the third culture space 2113, and the first elevation partition wall The microalgae in the first culture space 2111 can be moved thirdly to the second culture space 2112 by raising the 2121 and pushing the microalgae with the moving member 2122 (S22).

And when the third movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and lower the first elevation partition wall 2121 to block the second culture space 2112. The culture chamber 2100 is rotated to its original position, and the microalgae inoculated with the seed can be supplied to the first culture space 2111 (S10).

Next, microalgae are cultured in the first culture space 2111 to the fourth culture space 2114, and when the culture is complete, the microalgae in the fourth culture space 2114 are moved to the fifth culture space 2115. Make it (S50). The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. When the cultivation is completed (S51), the culture chamber 2100 may be positioned to be inclined while axially rotated by the lifting means 3000. Thereafter, the fourth elevating partition wall 2121 is raised and the microalgae in the fourth culture space 2114 can be first moved to the fifth culture space 2115 by pushing the microalgae with the moving member 2122 (S52). . Accordingly, the microalgae can move from the fourth culture space 2114 to the fifth culture space 2115 by gravity.

In addition, when the first movement is completed, it is checked whether the fourth culture space 2114 is empty (S53), the fourth elevation bulkhead 2121 is lowered to block the fifth culture space 2115, and the third elevation bulkhead The microalgae in the third culture space 2113 can be secondarily moved to the fourth culture space 2114 by raising the 2121 and pushing the microalgae with the moving member 2122 (S42).

In addition, when the second movement is completed, it is checked whether the third culture space 2113 is empty (S43), the third elevation bulkhead 2121 is lowered to block the fourth culture space 2114, and the second elevation bulkhead The microalgae in the second culture space 2112 can be moved thirdly to the third culture space 2113 by raising the 2121 and pushing the microalgae with the moving member 2122 (S32).

In addition, when the third movement is completed, it is checked whether the second culture space 2112 is empty (S33), the second elevation partition wall 2121 is lowered to block the third culture space 2113, and the first elevation partition wall By raising the 2121 and pushing the microalgae with the moving member 2122, the microalgae in the first culture space 2111 can be moved to the second culture space 2112 for the fourth time (S22).

Then, when the fourth movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and lower the first elevation partition wall 2121 to block the second culture space 2112. The culture chamber 2100 is rotated to its original position, and the microalgae inoculated with the seed can be supplied to the first culture space 2111 (S10).

Next, microalgae are cultured in the first culture space 2111 to the fifth culture space 2115, and when the culture is complete, the microalgae in the fifth culture space 2115 are moved to the sixth culture space 2116. Make it (S60). The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. When the cultivation is completed (S61), the culture chamber 2100 may be positioned to be inclined while axially rotated by the lifting means 3000. Thereafter, the fifth lifting partition wall 2121 is raised and the microalgae in the fifth culture space 2115 can be firstly moved to the sixth culture space 2116 by pushing the micro-members with the moving member 2122 (S62). Accordingly, the microalgae can move from the fifth culture space 2115 to the sixth culture space by gravity.

When the first movement is completed, it is checked whether the fifth culture space 2115 is empty (S63), the fifth elevation bulkhead 2121 is lowered to block the 6th culture space 2116, and the fourth elevation bulkhead 2121 ) And pushing the microalgae with the moving member 2122 to move the microalgae in the fourth culture space 2114 to the fifth culture space 2115 (S52).

In addition, when the second movement is completed, it is checked whether the fourth culture space 2114 is empty (S53), the fourth elevation bulkhead 2121 is lowered to block the fifth culture space 2115, and the third elevation bulkhead The microalgae in the third culture space 2113 can be moved thirdly to the fourth culture space 2114 by raising the 2121 and pushing the microalgae with the moving member 2122 (S42).

In addition, when the third movement is completed, it is checked whether the third culture chamber 2100 is empty (S43), the third lifting bulkhead 2121 is lowered to block the fourth culture space 2114, and the second lifting bulkhead ( It is possible to move the microalgae in the second culture space 2112 to the third culture space 2113 for the fourth time by raising the 2121 and pushing the microalgae with the moving member 2122 (S32).

In addition, when the fourth movement is completed, it is checked whether the second culture space 2112 is empty (S33), the second elevation bulkhead 2121 is lowered to block the third culture space 2113, and the first elevation bulkhead By raising the 2121 and pushing the microalgae with the moving member 2122, the microalgae in the first culture space 2111 can be moved to the second culture space 2112 for the fourth time (S22).

And when the fifth movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and lower the first elevation partition wall 2121 to block the second culture space 2112. The culture chamber 2100 is rotated to its original position, and the microalgae inoculated with the seed can be supplied to the first culture space 2111 (S10).

Finally, microalgae are cultured in the first culture space 2111 to the sixth culture space 2116, and when the culture is completed, the microalgae are discharged from the sixth culture space 2116 as shown in FIG. 10 to be harvested. (S70), and microalgae from the fifth culture space (2115) to the sixth culture space (2116), from the third culture space (2113) to the fourth culture space (2114), and from the second culture space (2112) The third culture space 2113 may be moved from the first culture space 2111 to the second culture space, and microalgae inoculated with the seeds may be supplied to the first culture space 2111. The microalgae in each culture chamber 2100 may be cultured for a predetermined time under the corresponding optimal culture conditions. In addition, when the culture is completed in each culture chamber 2100 (S71), the culture chamber 2100 may be positioned to be inclined while rotating axially by the lifting means 3000. Thereafter, the sixth lifting partition wall 2121 may be raised to discharge and harvest the microalgae in the sixth culture space 2116 (S72). Accordingly, the microalgae may be discharged from the sixth culture space 2116 to the outside by gravity.

When harvesting is complete, it is checked whether the 6th culture space 2116 is empty (S73), the 6th elevating bulkhead 2121 is lowered to block the outside and the 6th cultured space 2116, and the 5th elevating bulkhead 2121 ) And pushes the unmanufactured birds with the moving member 2122 to first move the microalgae in the fifth culture space 2115 to the sixth culture space 2116 (S62).

In addition, when the first movement is completed, it is checked whether the fifth culture space 2115 is empty (S63), the lifting bulkhead 2121 is lowered to block the sixth culture space 2116, and the fourth lifting bulkhead ( The microalgae in the fourth culture space 2114 can be secondarily moved to the fifth culture space 2115 by raising the 2121 and pushing the microalgae with the moving member 2122 (S52).

In addition, when the second movement is completed, it is checked whether the fourth culture space 2114 is empty (S53), the fourth elevation bulkhead 2121 is lowered to block the fifth culture space 2115, and the third elevation bulkhead The microalgae in the third culture space 2113 can be moved thirdly to the fourth culture space 2114 by raising the 2121 and pushing the microalgae with the moving member 2122 (S42).

In addition, when the third movement is completed, it is checked whether the third culture space 2113 is empty (S43), the third elevation bulkhead 2121 is lowered to block the fourth culture space 2114, and the second elevation bulkhead By raising the 2121 and pushing the microalgae with the moving member 2122, the microalgae in the second culture space 2112 can be moved fourthly to the third culture space 2113 (S32).

In addition, when the fourth movement is completed, it is checked whether the second culture space 2112 is empty (S33), the second elevation bulkhead 2121 is lowered to block the third culture space 2113, and the first elevation bulkhead By raising the 2121 and pushing the microalgae with the moving member 2122, the microalgae in the first culture space 2111 can be moved to the second culture space 2112 for the fourth time (S22).

And when the fifth movement is completed, it is possible to check whether the first culture space 2111 is empty (S23), and lower the first elevation partition wall 2121 to block the second culture space 2112. The culture chamber 2100 is rotated to its original position, and the microalgae inoculated with the seed can be supplied to the first culture space 2111 (S10).

On the other hand, to complete the cultivation and harvesting of microalgae, the microalgae inoculated with the seed are no longer supplied to the first culture space 2111, and the microalgae from the previous culture chamber 2100 are transferred to the next culture chamber 2100. And repeat the process of harvesting through the last culture chamber 2100.

In addition, the bottom surface area of the first culture space 2111 may be the narrowest and gradually widen, and the bottom surface area of the sixth culture space 2116 may be the widest.

In addition, in the above-described processes (S10 to S70), the culture conditions of the culture chamber 2100, the determination of the completion of the culture, the operation of the lifting means 3000, the first lifting partition walls 2121 to the sixth lifting partition walls 2121 The operation and determination of all components, including operations, etc., can be made by the control unit. In addition, the culture time of each culture chamber 2100 is set to be the same, and when it is confirmed that the subsequent culture is complete, it is determined that the previous culture is also completed, and the next process can be performed. In addition, regardless of the cultivation time, the next process may be performed only after both the subsequent and pre-cultures are completed.

Meanwhile, the above-described process may be implemented so that a worker can wirelessly operate through a smartphone, tablet, notebook, or PC. Briefly, in the culture of each process, various information including light source, illuminance, temperature, humidity and carbon dioxide are collected by various sensors and the progress is monitored, and the collected information is analyzed in real time in a computer and then analyzed by a control device. It can be installed to adjust the growth conditions and environment of microalgae.

In addition, the monitoring and control system can be monitored and managed with a smartphone through web-based software, and when an abnormality occurs, an alarm function by sending a text (including SMS) can be configured. At this time, the control system may be configured such that a function of sending a warning when an abnormality symptom falls below or exceeds a value set for each sensor can be expanded.

In addition, sensing and control may be possible through embedded systems such as light sources, cell growth, temperature, humidity, carbon dioxide, illuminance, wavelength, and video images for specialized control of microalgae for the production of bioactive substances. At this time, the output of the sensor used is an analog and digital output, which is integrated through a multi-channel DAC board and controls the light source, cell growth, temperature, humidity, carbon dioxide, illuminance, and wavelength according to the information to realize an optimized culture environment. I can. In addition to these conditions, it is possible to install a composite LED module in which LEDs for each wavelength are combined in order to control the wavelength and illuminance optimized for microalgae growth in each process by manufacturing LED design and modules. In addition, the heat generated from the LED can be configured in a way that is controlled in conjunction with the coolant for temperature control to save energy.

As described above, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. Therefore, the above-described embodiments are illustrative in all respects and should be understood as non-limiting. The scope of the present invention is indicated by the claims to be described later rather than by the detailed description, and all changes or modified forms derived from the meaning and scope of the claims and equivalent concepts should be interpreted as being included in the scope of the present invention. do.

1000: base
1100: rotating shaft
2000: Culture Department
2100: culture chamber 2110: culture space
2111: first culture space 2112: second culture space
2113: 3rd culture space 2114: 4th culture space
2115: 5th culture space 2116: 6th culture space
2120: bulkhead means 2121: elevating bulkhead
2122: moving member 2123: moving bulkhead
2124: magnetic material 2125: iron plate
2200: lighting means
2210: first light source 2220: sixth light source.
3000: means of lifting.

Claims (7)

In a continuous culture device designed to cultivate microalgae,
Including; a partition wall means 2120 and a culture unit 2000 having a culture chamber 2100 in which the culture spaces 2110 are divided for each culture process of microalgae by the partition means 2120,
The partition wall means 2120 is installed to open and close the neighboring culture spaces 2110 by raising and lowering the lifting partition wall 2121 to move the microalgae in the culture space 2110 to the next culture space 2110, or move It is installed to move the microalgae from the culture space to the next culture space by moving the partition wall 213,
The partition wall means 2120 further includes a magnetic body 2124 installed to move the moving partition 213 arranged to be divided into a plurality of culture spaces 2110 by magnetic force,
Flow control type continuous microalgae, characterized in that the moving partition wall 213 and the magnetic body 2124 are installed to move the microalgae for each culture process by pushing the microalgae, and when discharging the microalgae, they are installed to move to partition the first culture space 2110. Culture device.
In claim 1,
The culture spaces 2110 flow control type continuous microalgae culture apparatus, characterized in that the bottom surface area is different for each culture process.
In claim 1 or 2,
The partition wall means 2120 is arranged to be adjacent to the lifting partition wall 2121 installed to lift while being divided into a plurality of culture spaces 2110, and to push the microalgae in the culture space 2110 to the next culture space 2110. Flow control type continuous microalgae culture apparatus, characterized in that further comprising a moving member (2122) installed.
In paragraph 3,
A base 1000 installed so that one side portion of the culture chamber 2100 rotates while the culture chamber 2100 is seated; And
An elevating means (3000) installed to rotate the culture chamber (2100) obliquely to the axis; further comprising,
Flow control type continuous microalgae culture apparatus, characterized in that the microalgae in the culture space (2110) is moved to the next culture space by gravity.
delete In the method of culturing microalgae step by step in a plurality of culture spaces,
A supply step of supplying the microalgae inoculated with the seed into the first culture space 2110 provided in the culture chamber 2100 by the partition wall means 2120;
A moving and supplying step of moving the microalgae in the first culture space 2110 to the next culture space 2110, and supplying the microalgae inoculated with the seed in the first culture space 2110;
A repeating step of repeating the moving and supplying steps; and
Including; discharge step of discharging the cultured microalgae from the final culture space (2110),
The partition wall means 2120 sequentially raises the lifting partition wall 2121 so that the neighboring culture spaces 2110 pass in sequence, and the moving member 2122 reciprocates to move the microalgae in the culture space 2110 to the next. Move to the culture space 2110 or push the microalgae as the moving partitions 213 move and move to the next culture space 2110,
The culture chamber 2100 is inclined and the lifting partition wall 2121 is raised so that the microalgae are moved from the culture space 2110 to the next culture space 2110 by gravity, or
The moving partition wall 213 is in contact with the bottom of the culture chamber 2100 by a magnetic body 2124 to move by rolling or sliding,
Flow-controlled continuous microalgae culture method, characterized in that. delete

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