KR20200072427A - Composition for promoting sweet potato rooting containing NAA and potassium nitrate as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a composition for promoting rooting of sweet potatoes, comprising 1-Naphthaleneacetic acid (NAA) and potassium nitrate (KNO_3) as active ingredients. Since the composition of the present invention can promote rapid root development of sweet potato seedlings, the productivity of sweet potato seedlings is increased, and an effect of cost reduction can also be expected.

Description

Composition for promoting sweet potato rooting containing NAA and potassium nitrate as effective component and uses thereof}

The present invention relates to a composition for promoting rooting of sweet potatoes containing NAA and potassium nitrate as an active ingredient and uses thereof.

In general, it is known that the growth of early roots after planting has a great influence on the survival of the sweet potato seedlings as well as the growth of the sweet potato seedlings. In addition, in recent farms, the use of sweet potato virus disease free has increased significantly, and in the case of the virus disease free disease, it is multiplied by the winter shoveling method. Doing. Therefore, there is a method of increasing the temperature at night to enhance rooting and rooting, but as the temperature increases, the oil ratio is additionally generated, which is a great burden on the farm, and rapid root development and rooting of sweet potato seedlings are sweet potato seedlings (soonsun) In addition to increasing productivity in production, it has a significant impact on cost savings.

A number of studies have shown that auxin plays a leading role in plant root development, and auxin has a significant effect on the development of lateral root and root hair as well as the primary root. (Benfey et al., 2010 Plant J. 61:992-1000; Casimiro et al., 2003 Trends Plant Sci . 8:165-171). Plant rooting agents using auxin have been commercialized as Roton and have been used in various plants. On the other hand, in auxins, the effect on rooting appears to show a large difference depending on the substance (Metaxas et al., 2004 Plant Growth Regulation 44:257-266), and the present inventors use various auxins to be suitable for rooting sweet potato seedlings. It was intended to find out the type and concentration of auxin.

On the other hand, Korean Registered Patent No. 1924441 discloses a'medium for rooting pear sprouts' containing NAA (1-Naphthaleneacetic acid) and IBA (indole-3-butyric acid), and Korean Patent Publication No. 2004-0027907 Although a'lactone derivative and a plant growth regulator and rooting inducing agent using the same as an active ingredient' have been disclosed, the composition for promoting rooting of sweet potato containing NAA and potassium nitrate of the present invention as an active ingredient and its use are not described.

The present invention was derived by the above-described needs, and the present inventor treats four types of auxins (NAA, K-NAA, IBA and K-IBA) with sweet potato seedlings at various concentrations in order to promote root development of sweet potatoes. And, as a result of confirming the root development, it was confirmed that the root development of sweet potato seedlings was the best under the NAA treatment condition of 5 mg/ℓ, and the result was treated by mixing 1.8 g/ℓ of potassium nitrate with NAA at the concentration, and the NAA treatment alone. Compared with NAA and potassium nitrate, the present invention was completed by confirming that root development of sweet potato seedlings was improved by about 30% in the mixed treatment group.

In order to solve the above problems, the present invention provides a composition for promoting rooting of sweet potatoes containing NAA (1-Naphthaleneacetic acid) and potassium nitrate (KNO ₃ ) as active ingredients.

In addition, the present invention provides a method for promoting the rooting of sweet potatoes comprising the step of treating the composition for promoting rooting to sweet potato seedlings.

In addition, the present invention provides a root sprouting enhanced sweet potato seed sprout produced by a method and a method for producing a root sprout enhanced sweet potato seedling comprising the step of seedling the sweetener seedling after treating the composition for promoting rooting.

By using the composition for promoting sweet potato rooting according to the present invention, it is possible to promote the rapid root development of sweet potato seedlings, thus shortening the growth period of sweet potato seedlings (end of season), increasing productivity, and also expecting an effect of cost reduction. It is thought to be able to contribute to improving the income of sweet potato-growing farmers.

1 is a result of root development analysis of sweet potato seedlings according to the type and treatment concentration of auxin, A is NAA (1-naphthaleneacetic acid), B is K-NAA (1-naphthaleneacetic acid, potassium salt), C is IBA (indole -3-butyric acid) and D were treated with K-IBA (indole-3-butyric acid, potassium salt), and changes over time in the number of roots by concentration were observed. Each data represents mean±standard deviation.
Figure 2 is the analysis of the root development of sweet potato seedlings in NAA alone treatment and NAA and KNO ₃ mixed treatment, A is 2 days after the treatment (NT) and NAA 1.0, 2.5, 5.0 or 10.0 ㎎ / ℓ treatment alone and NAA 1.0, 2.5, 5.0, 10.0 ㎎/ℓ + KNO ₃ 1.8 g/ℓ This photograph shows the root development of the mixed treatment. B is the number of roots of each treatment group after 4 days of treatment, and each data represents the mean±standard deviation.
FIG. 3 shows that KN-NA5 (mixed with NAA 5 mg/L and KNO ₃ 1.8 g/L) selected as a sweet potato-only rooting agent is more effective in root development of sweet potato than commercially available rooting agent product Rooton. Each data represents mean±standard deviation.

In order to achieve the object of the present invention, the present invention provides a composition for promoting rooting of sweet potatoes containing NAA (1-Naphthaleneacetic acid) and potassium nitrate (KNO ₃ ) as active ingredients.

In the composition for promoting rooting of sweet potatoes according to the present invention, the NAA and potassium nitrate may be included in concentrations of 3 to 7 mg/L and 1.5 to 2.5 g/L, respectively, preferably NAA and potassium nitrate. It may be included in concentrations of 4 to 6 mg/L and 1.6 to 2.0 g/L, respectively, and more preferably, NAA and potassium nitrate may be included in concentrations of 5 mg/L and 1.8 g/L, respectively. , But is not limited to this.

The composition for promoting sweet potato rooting of the present invention is characterized in that the root development shows an excellent effect of about 30% in the mixed treatment condition of NAA and potassium nitrate compared to the treatment of NAA alone.

NAA is a synthetic auxin that has a physiological effect such as IAA (Indole-3-acetic acid), a naturally occurring auxin hormone, and is used as a plant growth regulator. Auxin is a plant hormone that plays a major role in plant growth, and the concentration that can promote root growth is less than one tenth of the concentration of stem growth. Became. As such, the function of auxin can be reversed depending on the crop or the concentration, so it is important to find a suitable treatment concentration suitable for the intended use.

In the composition of the present invention, the promotion of rooting may be preferably an increase in the number of roots of sweet potato seedlings, but is not limited thereto.

The present invention also provides a method for promoting the rooting of sweet potatoes comprising the step of treating the composition for promoting rooting of the present invention to sweet potato seedlings.

In the method for promoting rooting of sweet potatoes according to an embodiment of the present invention, the treatment is performed on the composition for promoting rooting of sweet potato seedlings containing 3 to 7 mg/ℓ of NAA and 1.5 to 2.5 g/l of potassium nitrate. It may be immersed for 7 minutes, but is not limited thereto. Sweet potato seedlings grown for 3 to 4 days after treating the composition for promoting rooting according to the present invention are characterized in that the number of roots is increased by 30% compared to the composition untreated sweet potato seedling for promoting rooting.

The present invention also provides a method for producing sweet potato seedlings with improved root development, comprising the step of seedling the sweet potato seedlings after treating the composition for promoting rooting of the present invention, and providing sweet potato seedlings with improved root development. do.

In the production method according to an embodiment of the present invention, the composition for promoting rooting includes 3 to 7 mg/L of NAA and 1.5 to 2.5 g/L of potassium nitrate, and the composition for promoting rooting is treated and Seedling of sweet potato seedlings has the effect of significantly increasing root development and shortening the growth period of the seedlings and increasing productivity.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

1. Analysis of the effect of auxin type and treatment concentration on root development of sweet potato seedlings

For sweet potato seedlings, seedlings with a size of about 25 to 30 cm, which were collected for farming from the farmhouse, were used for the experiment. The auxins used in the experiment are NAphthaleneacetic Acid (NAA), Naphthaleneacetic Acid (K-NAA), Potassium Salt (indole-3-butyric acid), IBA (indole-3-butyric acid, potassium salt), and the substance These were prepared to be 0, 0.5, 1.0, 2.5, 5.0, and 10.0 mg/l, respectively, so that one end of the resection of the seedlings collected was soaked for about 5 minutes, and then immersed in garden soil. Each of the sweet potato seedlings treated with the drug was randomly collected 5 weeks from the next day after treatment, and the number of roots generated was examined to observe the change over time, and the previously investigated seedlings were discarded and were not reused in the experiment.

2. NAA treatment and NAA and potassium nitrate (KNO ₃ ) Analysis of the effect of mixed treatment on root development of sweet potato seedlings

For sweet potato seedlings, seedlings with a size of about 25 to 30 cm, which were collected for planting at a farmhouse, were used for the experiment, and were immersed in the soil by immersion in NAA alone or a mixed solution of NAA + KNO ₃ for 5 minutes. NAA was prepared to be 0, 1.0, 2.5, 5.0 and 10.0 mg/L, respectively, and KNO ₃ was prepared to be 1.8 g/L, and used in the experiment. Analysis of the effect on root development was compared by analyzing the number of roots after extracting each sweet potato seedling after 4 days by inserting them into the topsoil for horticultural treatment.

3. NAA 5 mg/ℓ and KNO ₃ Analysis of the effect of 1.8 g/ℓ mixed treatment (KN-NA5) on sweet potato root development

For sweet potato seedlings, seedlings with a size of about 25 to 30 cm, which were collected for planting at a farmhouse, were used in the experiment, and KNO ₃ 1.8 g/ℓ + NAA 5 mg/ℓ which was the most effective for root development of sweet potatoes through previous experiments was mixed After treatment with (KN-NA5) and NAA 5 mg/ℓ alone, and a commercialized rooting agent, Roton (component name: Naphthylacetamide), the number of roots was observed over time and compared. In the treatment of KN-NA5 and NAA 5 mg/ℓ, 1 section of the sweet potato seedling was immersed for about 5 minutes, and Luton was subjected to coating processing.

Example 1. Analysis of the effect of the type and treatment concentration of auxin on the root development of sweet potato seedlings

Analysis of the effect of the type and treatment concentration of auxin on the root development of sweet potato is shown in FIG. 1. In the case of NAA treatment, an increase in the number of roots was observed at a concentration of 2.5 and 5.0 mg/L from the third day, and the root development was best at a concentration of 5.0 mg/L (FIG. 1A). However, it was confirmed that under the NAA treatment conditions of 10.0 mg/L, root development was poorer than the NAA treatment conditions of 5.0 mg/L. On the other hand, in the case of K-NAA treatment, the root development tended to be good at the concentration of 0.5 mg/ℓ on the second day of treatment and 2.5 or 5.0 mg/ℓ on the third day of treatment, but overall there was no significant difference between treatments. Did (Fig. 1B). IAA treatment showed the highest root development at a concentration of 5.0 ㎎/ℓ (FIG. 1C ), and in the K-IBA treatment group, root development was good at a concentration of 10 ㎎/ℓ until the third day and 2.5 ㎎/ℓ on the fourth day. Appeared (Figure 1D).

Looking at the differences in root development of sweet potato seedlings according to the type of synthetic auxin, overall, the root development was the best in the NAA treatment group. Next, K-IBA showed a rather high trend, but IAA treatment or K-NAA treatment And did not show a big difference.

Example 2. NAA treatment alone and NAA and KNO ₃ Analysis of the effect of mixed treatment on root development of sweet potato seedlings

In the present invention, NAA was found to be the most effective for root development of sweet potatoes (FIG. 1), and these results are metaxas that showed good root development in the order of K-IBA, IBA, IAA, and NAA in the shoveling experiment on strawberry trees. Et al. (2004 Plant Growth Regulation 44: 257-266). On the other hand, in Example 1, KNO ₃ was added to NAA, which was most effective in root development of sweet potato seedlings, to perform a comparative experiment on root development.

As a result, more roots were observed in the condition treated by mixing KNO ₃ than in NAA alone treatment (FIG. 2A ), and after 4 days of treatment, the number of roots was examined, and NAA 5 mg/ℓ and KNO ₃ 1.8 g/ℓ In the mixed treatment group, root development was about 30% higher than that of the NAA alone treatment group (FIG. 2B ). Accordingly, a mixture of NAA 5 mg/ℓ and KNO ₃ 1.8 g/ℓ (hereinafter KN-NA5) was selected as a rooting agent for sweet potato seedlings.

Example 3. Analysis of the effect of KN-NA5 on sweet potato root development

In the first and second experiments, KN-NA5, which was most effective for root development of sweet potato seedlings, and luton, which was commercialized as a rooting agent in existing horticultural crops, were compared. As a control, comparative analysis was performed including a control (NT) with no addition of auxin and a treatment with NAA 5.0 mg/L alone.

As a result, as shown in Figure 3, KN-NA5 treated group showed the best root development from the 3rd day of treatment, and on the 4th day, about 30% compared to the NAA 5.0 mg/ℓ single treatment group and about 20 compared to the Luton treatment group. It showed that the root number was as high as %, and it was confirmed that KN-NA5 is a very good rooting agent in root development of sweet potatoes.

Claims (6)

A composition for promoting rooting of sweet potatoes containing NAA (1-Naphthaleneacetic acid) and potassium nitrate (KNO ₃ ) as active ingredients. According to claim 1, wherein the NAA and potassium nitrate composition for promoting the rooting of sweet potatoes, characterized in that contained in a concentration of 3 to 7 mg/ℓ and 1.5 to 2.5 g/ℓ, respectively. A method for promoting rooting of sweet potatoes, comprising the step of treating the composition for promoting rooting of claim 1 or 2 with sweet potato seedlings. The method of claim 3, wherein the treatment is immersing sweet potato seedlings in a composition for promoting rooting for 3 to 7 minutes. A method for producing sweet potato seedlings with enhanced root development, comprising the step of seedling a sweet potato seedling after treating the composition for promoting rooting of claim 1 or claim 2. A sweet potato seed sprout with improved root development produced by the method of claim 5.

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