KR20200069761A - Method of preparing onion skin extract - Google Patents
Method of preparing onion skin extract Download PDFInfo
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- KR20200069761A KR20200069761A KR1020180157188A KR20180157188A KR20200069761A KR 20200069761 A KR20200069761 A KR 20200069761A KR 1020180157188 A KR1020180157188 A KR 1020180157188A KR 20180157188 A KR20180157188 A KR 20180157188A KR 20200069761 A KR20200069761 A KR 20200069761A
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- onion
- extraction
- onion skin
- extract
- quercetin
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- 241000234282 Allium Species 0.000 title claims abstract description 59
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- 238000000034 method Methods 0.000 title claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims description 28
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- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims description 6
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- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims description 4
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- 239000004155 Chlorine dioxide Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
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- 235000014655 lactic acid Nutrition 0.000 claims description 3
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- 239000000347 magnesium hydroxide Substances 0.000 claims description 3
- 229910001862 magnesium hydroxide Inorganic materials 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 3
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
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- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
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- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
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- 238000010298 pulverizing process Methods 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
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- 230000007103 stamina Effects 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
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- 238000004724 ultra fast liquid chromatography Methods 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/51—Concentration
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/57—Chemical peeling or cleaning of harvested fruits, vegetables or other foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2250/00—Food ingredients
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- A23V2250/21—Plant extracts
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- A23V2300/00—Processes
- A23V2300/14—Extraction
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- A23V2300/00—Processes
- A23V2300/31—Mechanical treatment
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/50—Concentrating, enriching or enhancing in functional factors
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Abstract
Description
본 발명은 쿼세틴을 함유하는 양파껍질 추출물을 제조하는 방법에 관한 것으로서, 알칼리수를 이용하여 공정비용을 현저히 절감시키고, 쿼세틴을 포함하는 고형분의 수율을 증대시키는 방법에 관한 것이다.The present invention relates to a method for preparing an onion peel extract containing quercetin, and to a method for significantly reducing the process cost using alkaline water and increasing the yield of solids containing quercetin.
양파는 백합과에 속하는 다년초로서 쿼세틴(Quercetin), 루틴(rutin) 등의 플라보노이드 물질이 풍부한 대표적 식품으로 알려져 있다. 양파는 재배역사가 오래되었고, 고유한 맛과 향기를 가지고 있어 고추, 마늘 등과 함께 전 세계적으로 널리 이용되고 있는 조미채소이다. 고대로부터 양파는 스태미너 증가에 효과가 있는 것으로 알려져 있어, 고대 이집트에서는 피라미드 건설 노동자들에게 양파를 배급하여 피로를 빨리 회복하도록 하였다라는 기록이 있으며, 전통 한의약에서도 양파가 한약재로 이용되어 해열, 구충, 해독 등의 효과를 나타냈다는 기록이 있다. Onion is a perennial plant belonging to the Liliaceae family and is known as a representative food rich in flavonoid substances such as quercetin and rutin. Onion is a seasoned vegetable that is widely used all over the world along with red pepper and garlic because it has a long history of cultivation and has a unique taste and fragrance. Since ancient times, onions are known to be effective in increasing stamina, and in ancient Egypt, there is a record that onion workers were distributed to pyramids to quickly recover fatigue, and in traditional Chinese medicine, onions are used as medicinal herbs. There are records that showed detoxification and other effects.
최근의 연구에 의하면 양파가 나타내는 생리적 기능성 효과는 황을 포함한 알릴화합물(Allyl compound)과 풍부한 플라보노이드에 의한 것으로 밝혀지고 있다. 쿼세틴(Quercetin)은 양파에 포함된 주된 플라보노이드이며, 단량체인 쿼세틴 아글리콘(Quercetin aglycone)과 글루코스와 결합된 쿼세틴 4-글리코사이드(Quercetin 4-glycoside) 등이 양파에 다량 포함되어 있는 것으로 알려져 있다. 쿼세틴은 다중 페놀고리를 함유하고 있는 분자구조를 가지고 있어 항산화활성이 뛰어나고 활성산소의 산화활동을 억제하거나 제거하는 능력이 매우 강하다. 또한 콜라겐에 의해 촉진되는 혈소판의 활성을 억제시켜 혈행 개선효과를 갖고 있는 것으로 유명하다. 양파의 가식부분에는 약 0.01%의 쿼세틴이 함유되어 있는 것으로 알려져 있으며, 겉껍질로 갈수록 함량이 증가하여 양파껍질부위에는 순 무게의 약 6.5%에 달하는 쿼세틴을 함유하고 있다고 보고되어 있다. Recent studies have shown that the physiological and functional effects of onions are caused by allyl compounds containing sulfur and abundant flavonoids. Quercetin is the main flavonoid contained in onions, and is known to contain a large amount of onion, such as the monomers quercetin aglycone and quercetin 4-glycoside combined with glucose. Quacetin has a molecular structure that contains multiple phenolic rings, so it has excellent antioxidant activity and has a very strong ability to inhibit or eliminate oxidative activity of free radicals. It is also famous for inhibiting the activity of platelets promoted by collagen and improving blood circulation. It is known that the edible portion of onion contains about 0.01% of quacetin, and its content increases as it goes to the outer skin, and it is reported that the onion skin part contains about 6.5% of the net weight.
본 발명은 공정비용을 현저히 절감시키고, 쿼세틴을 포함하는 고형분의 수율을 증대시킬 수 있는 양파껍질 추출물을 제조하는 방법을 제공함에 그 목적이 있다.An object of the present invention is to provide a method for preparing an onion peel extract capable of significantly reducing the process cost and increasing the yield of solids containing quercetin.
1. 분쇄된 양파 껍질을 pH 9 내지 12의 알칼리수로 추출하는 단계를 포함하는 양파 껍질 추출물의 제조 방법.1. Method for producing onion peel extract comprising the step of extracting the crushed onion peel with alkaline water having a pH of 9 to 12.
2. 위 1에 있어서, 양파 껍질을 3 내지 10 mm의 크기로 분쇄하여 상기 분쇄된 양파 껍질을 얻는 단계를 더 포함하는 방법.2. The method of 1 above, further comprising the step of pulverizing the onion peel to a size of 3 to 10 mm to obtain the crushed onion peel.
3. 위 1에 있어서, 양파의 최외곽으로부터 2 내지 5번째 층을 채취하여 상기 양파껍질을 얻는 단계를 더 포함하는 방법.3. The method of 1 above, further comprising the step of obtaining the onion skin by collecting the 2 to 5th layer from the outermost of the onion.
4. 위 1에 있어서, 상기 추출 전에 상기 분쇄된 양파 껍질을 차아염소산수, 과산화수소, 구연산, 에탄올, 이산화염소 및 젖산으로 이루어진 군에서 선택된 적어도 하나로 세척하는 단계를 더 포함하는 방법.4. The method of 1 above, further comprising washing the crushed onion skin prior to the extraction with at least one selected from the group consisting of hypochlorous acid, hydrogen peroxide, citric acid, ethanol, chlorine dioxide, and lactic acid.
5. 위 1에 있어서, 상기 알칼리수는 탄산나트륨, 탄산수소나트륨, 염화칼슘, 수산화마그네슘 및 소성칼슘으로 이루어진 군에서 선택된 적어도 하나의 수용액인 방법.5. The method of 1 above, wherein the alkaline water is at least one aqueous solution selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, calcium chloride, magnesium hydroxide and calcined calcium.
6. 위 1에 있어서, 상기 추출은 60 내지 100℃에서 1 내지 2시간 동안 수행되는 것인 방법.6. The method according to the above 1, wherein the extraction is performed at 60 to 100° C. for 1 to 2 hours.
7. 위 1에 있어서, 상기 추출물을 진공감압, 원심분리 및 한외여과로 이루어진 군에서 선택된 하나의 방법으로 농축하는 단계를 더 포함하는 방법.7. The method of 1 above, further comprising the step of concentrating the extract by a method selected from the group consisting of vacuum decompression, centrifugation and ultrafiltration.
본 발명의 제조방법은 쿼세틴을 함유하는 양파껍질 추출물을 제조하는 방법에 관한 것으로서, 알칼리수를 이용하여 공정비용을 현저히 절감시키고, 쿼세틴을 포함하는 고형분의 수율을 증대시킬 수 있다.The manufacturing method of the present invention relates to a method for preparing an onion skin extract containing quercetin, which can significantly reduce the process cost using alkaline water and increase the yield of solids containing quercetin.
도 1은 본 발명의 일 구현예에 따른 제조방법의 개략적인 공정도이다.1 is a schematic process diagram of a manufacturing method according to an embodiment of the present invention.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 분쇄된 양파껍질을 pH 9 내지 12의 알칼리수로 추출하는 단계를 포함하는 양파껍질 추출물의 제조 방법을 제공한다.The present invention provides a method for producing an onion skin extract comprising the step of extracting the crushed onion skin with alkaline water having a pH of 9 to 12.
상기 양파껍질은 양파의 심부를 제외한 외곽층을 말하는 것으로서, 상기 제조방법은 양파의 최외곽으로부터 채취하여 상기 양파껍질을 얻는 단계를 더 포함할 수 있다. The onion peel refers to the outer layer excluding the deep portion of the onion, and the manufacturing method may further include obtaining the onion peel by collecting from the outermost portion of the onion.
상기 단계는 양파의 최외곽으로부터 1 내지 6번째, 2 내지 6번째, 1 내지 5번째 또는 2 내지 5번째 층을 채취한 것일 수 있는데, 쿼세틴 함량의 양파 외곽층 분포도를 고려할 때 바람직하게는 2 내지 5번째 층에서 채취한 것일 수 있다.The step may be a 1 to 6th, 2 to 6th, 1 to 5th, or 2 to 5th layer taken from the outermost side of the onion, and preferably 2 to 2 when considering the distribution of the onion outer layer of quercetin content. It may be from the fifth layer.
상기 제조방법은 양파껍질을 일정 크기로 분쇄하여 상기 분쇄된 양파 껍질을 얻는 단계를 더 포함할 수 있고, 상기 일정크기는 1 내지 15mm, 1.5 내지 15mm, 2 내지 14mm, 2 내지 13mm, 3 내지 13mm, 3 내지 12mm, 3 내지 11mm 또는 3 내지 10 mm의 크기, 바람직하게는 3 내지 10mm의 크기일 수 있는데, 상기 범위를 초과 또는 미달하여 너무 크거나 작은 크기로 분쇄하는 경우 추출물 내 쿼세틴을 포함하는 고형분의 함량을 오히려 떨어뜨리는 결과를 낳아 수율을 저하시킬 수 있다.The manufacturing method may further include the step of obtaining the crushed onion skin by crushing the onion skin to a certain size, and the predetermined size is 1 to 15 mm, 1.5 to 15 mm, 2 to 14 mm, 2 to 13 mm, and 3 to 13 mm. , 3 to 12mm, 3 to 11mm or 3 to 10mm, preferably 3 to 10mm in size, when exceeding or not exceeding the above range, when crushing to a size that is too large or too small, containing quercetin in the extract The yield may be lowered by causing the content of the solid content to drop.
상기 제조방법은 상기 추출 전에 상기 분쇄된 양파껍질을 차아염소산수, 과산화수소, 구연산, 에탄올, 이산화염소 및 젖산으로 이루어진 군에서 선택된 적어도 하나로 세척하는 단계를 더 포함할 수 있고, 바람직하게는 포자균의 완전한 제거의 측면에서 차아염소산수로 세척하는 단계를 더 포함할 수 있다.The manufacturing method may further include the step of washing the crushed onion skin prior to the extraction with at least one selected from the group consisting of hypochlorous acid, hydrogen peroxide, citric acid, ethanol, chlorine dioxide, and lactic acid, preferably spores. In terms of complete removal, the method may further include washing with hypochlorous acid water.
상기 알칼리수는 pH 9 내지 12의 특정 pH 범위를 가지는 것으로서, 상기 pH는 당업계의 주지된 방법을 자유로이 선택하여 설정될 수 있고, 구체적으로는 알칼리성 화합물을 물에 용해시켜 설정된 것일 수 있다.The alkaline water has a specific pH range of 9 to 12, and the pH may be set by freely selecting a well-known method in the art, and may be specifically set by dissolving an alkaline compound in water.
상기 알칼리성 화합물은 탄산나트륨, 탄산수소나트륨, 염화칼슘, 수산화마그네슘 및 소성칼슘으로 이루어진 군에서 선택된 적어도 하나일 수 있으나, 반드시 이에 제한되지 아니하고, 상기 pH 9 내지 12의 범위를 설정할 수 있는 화합물이라는 특별히 제한되지 않는다.The alkaline compound may be at least one selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, calcium chloride, magnesium hydroxide and calcined calcium, but is not limited thereto, and is not particularly limited as a compound capable of setting the range of the pH 9 to 12 Does not.
상기 pH는 본 발명의 추출에 사용되는 알칼리수의 특정 pH 범위로서, pH 9보다 낮은 pH를 갖는 경우, 알칼리성이 다소 약하여 양파껍질 내에 존재하는 세포들의 세포벽이나 세포질을 효과적으로 분해시킬 수 없어 추출 수율이 낮아지는 단점이 있고, pH 12보다 높은 pH를 갖는 경우, 알칼리성이 너무 높아 양파껍질 내 유효성분들이 변성되고, 추출물을 식품에 사용할 수 없을 정도로 인체에 유해할 수 있다는 단점이 있다.The pH is a specific pH range of alkali water used for extraction of the present invention, and when it has a pH lower than pH 9, the alkalinity is slightly weak, and thus the cell wall or cytoplasm of cells present in the onion peel cannot be effectively decomposed, resulting in low extraction yield. There is a drawback, and when it has a pH higher than pH 12, the alkalinity is too high to denature the active ingredients in the onion peel, and there is a disadvantage that the extract can be harmful to the human body to the extent that it cannot be used for food.
상기 추출은 환류추출, 냉침추출, 초음파추출 및 압착추출로 이루어진 군에서 선택된 적어도 하나의 추출법을 이용하여 추출할 수 있고, 유효성분의 최대 수율을 위하여 상기 추출을 수회 반복적으로 수행할 수 있으나, 알칼리수를 이용하여 알칼리 조건의 추출이 가능한 방법이라면 당업계에 주지된 추출방법을 선택하여 자유로이 수행할 수 있다.The extraction may be extracted using at least one extraction method selected from the group consisting of reflux extraction, cold immersion extraction, ultrasonic extraction and compression extraction, and the extraction may be repeatedly performed several times for maximum yield of active ingredients, but alkaline water If it is possible to extract the alkali conditions using a method can be freely selected by selecting an extraction method well known in the art.
상기 추출은 40 내지 120, 45 내지 115, 50 내지 110, 55 내지 105 또는 60 내지 100℃의 온도에서 수행될 수 있고 반드시 이에 제한되는 것은 아니나, 양파껍질 내 유효성분의 용해도 및 유효성분의 변성방지를 고려할 때, 바람직하게는 60 내지 100℃의 온도에서 수행되는 것이 바람직하다.The extraction may be performed at a temperature of 40 to 120, 45 to 115, 50 to 110, 55 to 105 or 60 to 100°C, but is not necessarily limited thereto, solubility of the active ingredient in the onion peel and prevention of denaturation of the active ingredient Considering, it is preferably carried out at a temperature of 60 to 100 ℃.
상기 추출은 0.5 내지 5시간, 0.6 내지 4시간, 0.7 내지 3시간, 0.8 내지 3시간, 0.9 내지 3시간, 1 내지 3시간, 1 내지 2.5시간 또는 1 내지 2시간 수행될 수 있으나, 양파껍질 내 유효성분의 용해도 및 유효성분의 변성방지를 고려할 때, 1 내지 2시간 동안 수행되는 것이 바람직하다.The extraction may be performed for 0.5 to 5 hours, 0.6 to 4 hours, 0.7 to 3 hours, 0.8 to 3 hours, 0.9 to 3 hours, 1 to 3 hours, 1 to 2.5 hours or 1 to 2 hours, but within the onion peel When considering the solubility of the active ingredient and prevention of denaturation of the active ingredient, it is preferably performed for 1 to 2 hours.
상기 제조방법은 상기 추출물을 진공감압, 원심분리 및 한외여과로 이루어진 군에서 선택된 하나의 방법으로 농축하는 단계를 더 포함할 수 있으나, 추출물 내 유효성분들을 효과적으로 농축시킬 수 있는 방법이라면 특별한 제한없이 당업계의 주지된 방법을 선택하여 수행할 수 있고, 양파껍질 내 유효성분의 변성방지를 고려할 때 진공감압 또는 원심분리 방법으로 수행함이 바람직하다.The manufacturing method may further include the step of concentrating the extract by one method selected from the group consisting of vacuum decompression, centrifugation and ultrafiltration, but if the method capable of effectively concentrating the active ingredients in the extract is sugar without any particular limitation. It can be performed by selecting a well-known method in the industry, and it is preferable to perform the vacuum reduction or centrifugation method when considering the prevention of denaturation of the active ingredient in the onion peel.
상기 농축에 있어서 진공감압법을 선택하여 수행하는 경우, 양파껍질 내 유효성분 중 일성분인 쿼세틴의 수율을 고려하는 측면에서, 쿼세틴의 화학적 구조가 깨지지 않도록 8시간 이내에 공정을 완료하는 것이 바람직하나, 반드시 이에 제한되지는 아니한다.In the case where the vacuum pressure reduction method is selected in the concentration, it is preferable to complete the process within 8 hours so that the chemical structure of quercetin is not broken in view of the yield of quercetin, which is one component of the active ingredient in the onion peel, It is not necessarily limited to this.
본 발명의 제조방법은 양파껍질 내 쿼세틴을 포함하는 다량의 유효성분이 세포벽 안 세포질에 다량 분포하는 것에 착안하여, 에탄올이나 물을 용매로 사용하여 녹여내는 방법을 사용하지 않고, 알칼리수로 세포벽과 세포질을 녹여내어 파괴함으로써 세포질 안의 단백질, 인지질, 쿼세틴 등이 물에 분산되어 나오는 방법을 고안한 것이다. 본 발명의 제조방법을 사용할 경우 유기용매를 사용하지 않기 때문에 경제성이 높고 친환경적인 쿼세틴 함유 양파껍질 추출액을 얻을 수 있으며, 추출의 속도가 매우 빠르고, 유효성분의 수율 또한 매우 높아 산업적 이용가능성이 높다.The manufacturing method of the present invention focuses on the fact that a large amount of active ingredients containing quercetin in an onion peel is distributed in a large amount in the cytoplasm inside the cell wall, without using a method of dissolving ethanol or water using a solvent, and the cell wall and cytoplasm with alkaline water. It was designed to dissolve and destroy proteins, phospholipids, and quercetin in the cytoplasm. When using the manufacturing method of the present invention, since an organic solvent is not used, it is possible to obtain an eco-friendly and eco-friendly quacetin-containing onion peel extract, the extraction speed is very fast, and the yield of the active ingredient is also very high, so industrial availability is high.
이하, 본 발명을 구체적으로 설명하기 위해 실시예를 들어 상세하게 설명하기로 한다. Hereinafter, examples will be described in detail to specifically describe the present invention.
실시예 1. 실험방법Example 1. Experimental method
양파에서 껍질을 가장 외곽층에서 바깥쪽 4번째 층까지 벗겨내어 준비한 양파껍질 1.5kg을 온도 60℃로 설정된 열풍건조기에 넣고 12시간 동안 두어 완전히 건조하였다. 건조된 양파껍질은 5mm 간격으로 날이 설치되어 있는 세절기로 한 번 길게 자른 후 90° 회전하여 다시 세절기에 통과시켜 길게 잘라냄으로써, 가로 세로가 각각 5mm인 정사각형 또는 이와 유사한 형태의 양파껍질 분쇄물을 제조하였다. 분쇄된 양파껍질을 0.01% 차아염소산나트륨 수용액에 10분간 담가 살균한 다음, 정제수로 헹궈 차아염소산나트륨을 모두 씻어내었다. 세척한 양파껍질을 적당한 크기의 추출용 부직포자루에 넣고, 이 부직포자루와 미리 제조한 pH 12 이온화칼슘 수용액 20L를 40L 추출조에 넣고 진공추출농축기(MODEL: COSMOS660, 제조원: ㈜경서산업)를 사용하여 온도 80℃, 상압조건에서 2시간동안 추출하였다. 추출이 끝난 후 부직포자루를 건져내고 추출액을 별도의 용기에 옮겨 담은 다음, 다시 40L 추출조에 상기 부직포자루와 pH 12 이온화칼슘 수용액 20L를 함께 넣고, 동일한 추출과정을 2회 반복하였다. Peel the onion from the outermost layer to the outer 4th layer and put 1.5 kg of the prepared onion peel in a hot air dryer set at a temperature of 60°C and leave it to dry for 12 hours. The dried onion peels are cut into pieces with a blade installed at intervals of 5 mm once, and then rotated by 90° to pass through the slicing machine and cut for a long time, crushing square or similar onion peels with a width of 5 mm each. Water was prepared. The crushed onion skin was immersed in a 0.01% aqueous sodium hypochlorite solution for 10 minutes, and then rinsed with purified water to wash away sodium hypochlorite. Put the washed onion peel into a non-woven sack for extraction of suitable size, and put 20 L of a pre-prepared pH 12 ionized calcium aqueous solution into a 40 L extraction tank and use a vacuum extraction concentrator (MODEL: COSMOS660, manufacturer: Gyeongseo Industries). It was extracted for 2 hours at a temperature of 80°C and atmospheric pressure. After the extraction is completed, the nonwoven sack is removed, the extract is transferred to a separate container, and then the 40 L extraction tank is put together with the nonwoven sack and 20 L of a pH 12 aqueous calcium ion solution, and the same extraction process is repeated twice.
실험예 1의 시료 제조를 위해, 상기 제조된 양파껍질 추출액을 농축조에 옮겨 담고 진공추출농축기(MODEL: COSMOS660, 제조원: ㈜경서산업)를 사용하여 60℃에서 약 8시간 동안 진공감압농축을 하였다. 농축된 양파껍질추출물을 굴절당도계(PAL-α, 제조사: ATAGO)로 측정하여 2 brix가 되었을 때 농축을 종료하였다. 제조한 양파껍질추출농축액은 분무건조기(PL-8, 제조사: OKAWARA MFG, Co. LTD,.)로 분무건조하여 분말을 제조하였는데, 이때의 조건은 atomizer speed 15,000 rpm, outlet temperature 180℃ 이었다.For the preparation of the sample of Experimental Example 1, the prepared onion peel extract was transferred to a concentration tank and vacuum decompressed and concentrated at 60° C. for about 8 hours using a vacuum extraction concentrator (MODEL: COSMOS660, manufacturer: Gyeongseo Industry). The concentrated onion peel extract was measured with a refractometer (PAL-α, manufacturer: ATAGO) to complete concentration when 2 brix was reached. The prepared onion peel extract concentrate was spray-dried with a spray dryer (PL-8, manufacturer: OKAWARA MFG, Co. LTD,.) to prepare powders. The conditions were atomizer speed 15,000 rpm and outlet temperature 180°C.
실험예 2의 시료 제조를 위해, 상기 실험예 1의 시료 제조과정에서, 추출 과정까지는 동일하게 수행한 다음, 추출액을 500ml 원심분리기에 분주하여 나눠담은 후 원심분리기(Combi 508, 제조사: 한일과학)에 넣고 15분간 3000rpm의 속도로 원심분리를 실시하였다. 원심분리가 끝난 후 상등액을 따라내고 침전물을 분리하여, 침전물에 10배가 되는 정제수를 붓고 교반하여 물에 고르게 분산하였다. 이렇게 재분산된 용액을 분무건조기로 분무건조하여 분말로 제조하였다. 도 1에 상기 과정을 모식적으로 나타내었다.For the preparation of the sample of Experimental Example 2, in the sample preparation process of Experimental Example 1, the same was performed until the extraction process, and then the extract was dispensed by dividing it in a 500 ml centrifuge and centrifuged (Combi 508, Hanil Science) And centrifuged at 3000 rpm for 15 minutes. After the centrifugation was completed, the supernatant was decanted, and the precipitate was separated, and purified water, which was 10 times as much as the precipitate, was poured and stirred to be evenly dispersed in water. The redispersed solution was spray dried to prepare a powder. The process is schematically illustrated in FIG. 1.
대조구로서 실험예 1과 추출, 농축, 분무건조 과정은 동일하지만, 용매로 일반 정제수(pH 6~7)를 사용하여 제조한 시료(대조구 1)와 용매로서 95% 에탄올을 사용하여 제조한 시료(대조구 2)를 준비하였다. The control, extraction, concentration, and spray drying processes are the same as those of Experimental Example 1 as a control, but a sample prepared using normal purified water (pH 6-7) as a solvent (Control 1) and a sample prepared using 95% ethanol as a solvent ( Control 2) was prepared.
각 시료 내 고형분의 수율은 하기 수학식 1에 의해 계산되었고, 쿼세틴 함량은 HPLC(Shimadzu Prominence UFLC, 제조사: Shimadzu)에 시료를 20㎕ injection하여 분석하였는데 자세한 분석조건 및 이동상의 Gradient 조성은 하기 표 1, 2와 같다.The yield of solids in each sample was calculated by Equation 1 below, and the quercetin content was analyzed by injecting 20 μl of the sample into HPLC (Shimadzu Prominence UFLC, manufacturer: Shimadzu).Detailed analysis conditions and gradient composition of the mobile phase are shown in Table 1 below. , Equal to 2.
[수학식 1][Equation 1]
수율 (%) = 건조중량(g) / 추출전 양파껍질중량(g)Yield (%) = dry weight (g) / onion peel weight (g) before extraction
실시예 2. 실험결과Example 2. Experimental results
하기 표 3에서 실시예 1의 실험결과를 확인할 수 있는데, 이를 참조하면, 실험예 1, 2 고형분 수율이 대조구에 비해 월등히 높음을 확인할 수 있다. 이는 알칼리수를 용매로 사용하여 양파껍질 내 세포벽과 세포질을 녹여내어 파괴함으로써, 짧은 시간 내에 세포질 내 단백질, 인지질, 쿼세틴 등의 다양한 물질을 다량 수득할 수 있게 된 것으로 판단된다.In Table 3 below, the experimental results of Example 1 can be confirmed. Referring to this, it can be seen that the Experimental Examples 1 and 2 solids yields are significantly higher than those of the control. This is believed to be able to obtain a large amount of various substances such as proteins, phospholipids and quercetin in the cytoplasm within a short time by dissolving and destroying the cell wall and cytoplasm in the onion skin using alkaline water as a solvent.
또한, 실험예 1, 2의 고형분 내 쿼세틴의 함량이 대조구 2와 유사하거나 더 높은 수준을 보이고 있는데, 이는 통상 에탄올을 용매로 사용하는 경우 에탄올의 가격이 비싸고, 유기용매를 이용한 추출에 따른 폐수처리 비용과 화재 방지 설비 구비에 따른 제반 비용이 과다한 점을 고려할 때, 단순 알칼리수로 추출하여 이와 유사하거나 높은 수준의 쿼세틴 함량을 보인다는 것은 산업적으로 매우 유의미한 결과로 판단된다.In addition, the content of quercetin in the solid content of Experimental Examples 1 and 2 is similar to or higher than that of Control 2, which is usually expensive when ethanol is used as a solvent, and wastewater treatment according to extraction using an organic solvent. Considering that the cost and the various costs associated with the provision of fire protection facilities are excessive, it is judged as a very meaningful result in the industry to have similar or high levels of quercetin extracted by simple alkaline water.
Claims (7)
Method for producing an onion peel extract comprising the step of extracting the crushed onion skin with alkaline water having a pH of 9 to 12.
The method according to claim 1, further comprising the step of obtaining the crushed onion skin by crushing the onion skin to a size of 3 to 10 mm.
The method according to claim 1, further comprising the step of obtaining the onion skin by collecting the 2 to 5th layer from the outermost of the onion.
The method of claim 1, further comprising washing the ground onion skin prior to the extraction with at least one selected from the group consisting of hypochlorous acid, hydrogen peroxide, citric acid, ethanol, chlorine dioxide, and lactic acid.
The method according to claim 1, wherein the alkaline water is at least one aqueous solution selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, calcium chloride, magnesium hydroxide and calcined calcium.
The method according to claim 1, wherein the extraction is performed for 1 to 2 hours at 60 to 100 ℃.
The method according to claim 1, further comprising the step of concentrating the extract by one method selected from the group consisting of vacuum decompression, centrifugation and ultrafiltration.
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| JP2001139486A (en) * | 1999-11-11 | 2001-05-22 | Shinji Murata | Processed material of outer skin of onion |
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| JP2003095859A (en) * | 2001-09-27 | 2003-04-03 | Sansho Seiyaku Co Ltd | Pigmentation inhibitor, method for producing the same and use thereof |
| WO2003057700A1 (en) * | 2001-12-28 | 2003-07-17 | Sansho Seiyaku Co., Ltd. | Compositions containing pigmentatin inhibitor, use thereof and process for producing the same |
| KR101878413B1 (en) | 2016-12-23 | 2018-07-13 | 김점례 | Healthy mixed drinks using onion skin and herbal ingredients and their preparation method |
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| JP2001139486A (en) * | 1999-11-11 | 2001-05-22 | Shinji Murata | Processed material of outer skin of onion |
| KR20020086117A (en) * | 2001-05-11 | 2002-11-18 | 삼조쎌텍 주식회사 | Anti-oxidant containing alkali extract of non-fat rice bran and onion peel mixture |
| JP2003095859A (en) * | 2001-09-27 | 2003-04-03 | Sansho Seiyaku Co Ltd | Pigmentation inhibitor, method for producing the same and use thereof |
| WO2003057700A1 (en) * | 2001-12-28 | 2003-07-17 | Sansho Seiyaku Co., Ltd. | Compositions containing pigmentatin inhibitor, use thereof and process for producing the same |
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