KR20200068287A - Mujigae-tteok comprising beet, onion and spinach and producing method thereof - Google Patents

Mujigae-tteok comprising beet, onion and spinach and producing method thereof Download PDF

Info

Publication number
KR20200068287A
KR20200068287A KR1020180155079A KR20180155079A KR20200068287A KR 20200068287 A KR20200068287 A KR 20200068287A KR 1020180155079 A KR1020180155079 A KR 1020180155079A KR 20180155079 A KR20180155079 A KR 20180155079A KR 20200068287 A KR20200068287 A KR 20200068287A
Authority
KR
South Korea
Prior art keywords
spinach
onion
beet
rice cake
weight
Prior art date
Application number
KR1020180155079A
Other languages
Korean (ko)
Inventor
정미자
오은별
강수정
윤소연
김솔빈
Original Assignee
광주대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 광주대학교산학협력단 filed Critical 광주대학교산학협력단
Priority to KR1020180155079A priority Critical patent/KR20200068287A/en
Publication of KR20200068287A publication Critical patent/KR20200068287A/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying
    • A23L5/13General methods of cooking foods, e.g. by roasting or frying using water or steam
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/42Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/43Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives

Landscapes

  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a rainbow rice cake comprising beet, onion and spinach and a method for manufacturing the same. The rainbow rice cake comprising beet, onion and spinach of the present invention can prevent excessive insulin secretion by slowly raising blood sugar, and thus can be used as a snack for diabetics, and also has an antioxidant effect and an inhibitory effect on cranial nerve cell death, thereby having an effect of suppressing aging and preventing dementia.

Description

비트, 양파 및 시금치를 함유하는 무지개떡 및 그 제조방법 {Mujigae-tteok comprising beet, onion and spinach and producing method thereof}Rainbow rice cake containing beet, onion and spinach and its manufacturing method {Mujigae-tteok comprising beet, onion and spinach and producing method thereof}

본 발명은 비트, 양파, 시금치를 함유하는 무지개떡 및 그 제조방법에 관한 것으로, 보다 상세하게는 비트, 양파, 시금치를 함유시켜 각각 고유의 색상을 나타내도록 하고 동시에 항당뇨효과와 항산화효과를 갖도록 한 무지개떡과 이를 제조하는 방법에 관한 것이다.The present invention relates to a rainbow rice cake containing beet, onion, and spinach and a method for manufacturing the same, and more specifically, to contain beet, onion, and spinach, so as to exhibit unique colors and to have anti-diabetic and antioxidant effects at the same time. It relates to a rainbow rice cake and a method of manufacturing it.

당뇨병 환자의 급속한 증가는 전 세계적으로 공통된 보건학적 문제로 우리나라가 포함된 아시아 지역에서 가장 두드러진다. 특히 최근 들어 현대인들의 활동량이 적은 생활습관과 비만인구의 증가에 따라 제2형 당뇨병이 증가하고 있어 당뇨병은 주목해야 할 질환이 되었다.The rapid increase in diabetics is a common health problem worldwide, most notably in Asia, where Korea is included. In particular, diabetes has become a remarkable disease in recent years because type 2 diabetes is increasing due to an increase in lifestyle and obesity.

혈당이 높은 상태에서 치료를 받지 않아 고혈당인 상태가 지속되면 당뇨병으로 인한 여러가지 급, 만성 합병증이 발생하게 된다. 따라서 철저한 혈당관리는 합병증 예방을 위해 매우 중요하다.When high blood sugar level is maintained due to the lack of treatment in a high blood sugar state, various sudden and chronic complications caused by diabetes occur. Therefore, thorough blood sugar management is very important to prevent complications.

당뇨병 환자 및 당뇨 예방을 위해 식품이 혈당 수치에 미치는 영향을 수치화한 당지수(glycemic index, GI)는 인체가 50g의 당질을 얼마나 빨리 혈당으로 전환시키는지를 계량화한 수치로, GI 값이 높을수록 소화, 흡수되는 속도가 빠르고 식품 섭취 후 혈당치가 빠르게 높아진다. 한국에서 당뇨병 환자의 90% 이상을 차지하는 제2형 당뇨병 환자는 인슐린 저항성을 갖고 있어 GI가 높은 식품을 섭취할수록 더 많은 인슐린을 분비하여야 한다. 이러다 보면 인슐린을 생산해 내는 췌장이 과로하여 혈당 조절 기능이 떨어지는 악순환에 빠지게 된다. 이는 당뇨병 환자뿐만 아니라 혈당을 급격히 상승시키는 식품을 자주 섭취하는 정상인들도 당뇨병에 걸릴 위험성을 증가시킬 수 있다.The glycemic index (GI), which quantifies the effect of food on blood sugar levels to prevent diabetes and diabetes, is a measure of how quickly the body converts 50 g of sugar into blood sugar. , The absorption rate is fast and the blood sugar level increases rapidly after eating food. In Korea, type 2 diabetes, which accounts for more than 90% of diabetics, has insulin resistance, so the more GI you eat, the more insulin you need to secrete. If you do this, the pancreas that produces insulin is overworked, leading to a vicious cycle of poor blood sugar control. This may increase the risk of diabetes, not only diabetics, but also normal people who frequently eat foods that increase blood sugar rapidly.

비트에는 베타인이라는 색소가 포함되어 있어 혈관 내 독소를 배출하고, 혈당을 낮추는 효과가 있다. 또한 세포 손상을 억제하고 토마토의 8배에 달하는 항산화 작용으로 폐암, 폐렴 등 암을 예방하고 염증을 완화시킨다. 비트의 8%는 염소로 구성되어 있는데 이 염소 성분은 간 정화작용을 하고, 골격 형성 및 유아 발육에 효과가 있다. 더불어 철분과 비타민이 다량 함유되어 있어 적혈구 생성을 돕고, 혈액을 맑게 하여 월경 불순이나 갱년기 여성에게 좋다. 뿐만 아니라 위 손상을 막아주고, 위 점막을 보호해주기도 한다.Beetin contains a pigment called betaine, which releases toxins from blood vessels and lowers blood sugar. In addition, it suppresses cell damage and prevents cancer, such as lung cancer and pneumonia, and relieves inflammation with antioxidant activity that is 8 times that of tomatoes. 8% of the beet is composed of chlorine, and this chlorine component acts as a liver cleansing agent and is effective for skeletal formation and infant development. In addition, it contains a large amount of iron and vitamins to help the production of red blood cells, clear the blood is good for menstruation and menopausal women. In addition, it prevents stomach damage and protects the gastric mucosa.

양파에는 퀘르세틴 성분이 풍부한데 양파의 퀘르세틴 성분은 지방과 콜레스테롤이 혈관에 축적되는 것을 억제하여 고혈압 예방에 도움을 준다. 또한 퀘르세틴은 활성산소와 과산화지질로부터 세포가 공격당하는 것을 막아주는 역할을 하며 세포의 염증 및 상처를 회복하는 데 효과가 있다. 또한 양파에는 크롬이 풍부하게 들어있는데, 크롬은 포도당 대사의 항상성을 유지하는 미량 무기질이며, 인슐린 작용을 촉진하므로 혈당 조절에 도움이 된다.Onion is rich in quercetin, and onion quercetin helps prevent hypertension by inhibiting the accumulation of fat and cholesterol in blood vessels. In addition, quercetin plays a role in preventing cells from being attacked from free radicals and lipid peroxide, and is effective in repairing inflammation and wounds of cells. In addition, onions are rich in chromium. Chromium is a trace mineral that maintains homeostasis of glucose metabolism and promotes insulin action, which helps regulate blood sugar.

시금치에는 마그네슘이 많이 함유되어 있는데 시금치의 마그네슘은 인슐린 분비와 작용 개선으로 혈당과 혈압, 콜레스테롤 관리, 혈관 합병증 감소효과가 있다. 당뇨 합병증 치료제에 들어있는 알파리포산이 시금치에도 들어있어 시금치는 당뇨 환자에게 유익한 채소이다. 코티졸 호르몬수치를 조절해 스트레스를 줄여 정서적 안정의 효과가 있고 췌장 인슐린 분비를 조절해주는 효과가 있다. 또한 나트륨 함량을 감소시킬 수 있으며, 지방을 감소시키기 때문에 당뇨병의 큰 원인 중 하나인 비만도 개선이 가능하다.Spinach contains a lot of magnesium, but magnesium in spinach has the effect of reducing blood sugar, blood pressure, cholesterol, and vascular complications by improving insulin secretion and action. Alpha-lipoic acid, which is included in the treatment for diabetes complications, is also present in spinach, which makes it a beneficial vegetable for diabetics. It regulates cortisol hormone levels to reduce stress, thereby improving emotional stability and controlling pancreatic insulin secretion. In addition, it is possible to reduce the sodium content, and because it reduces fat, obesity, one of the major causes of diabetes, can be improved.

떡은 곡식가루를 찌거나 삶아서 만든 음식으로, 그중 백설기는 멥쌀가루에 물 또는 설탕물을 내려서 시루에 앉혀 찐 떡이다. 백설기와 같은 떡은 GI 지수가 높아 당뇨환자가 섭취하는데 어려움이 있다.Rice cakes are foods made by steaming or boiling grain flour. Among them, Baekseolgi is steamed rice cake made by pouring water or sugar water on uncooked rice flour and sitting on a siru. Rice cakes such as snow white have a high GI index, making it difficult for diabetics to consume.

대한민국 특허공개 제10-2005-0075892호Republic of Korea Patent Publication No. 10-2005-0075892 대한민국 특허공개 제10-2007-0074313호Republic of Korea Patent Publication No. 10-2007-0074313 대한민국 특허공개 제10-2014-0016025호Republic of Korea Patent Publication No. 10-2014-0016025 대한민국 특허공개 제10-2015-0140899호Republic of Korea Patent Publication No. 10-2015-0140899 대한민국 특허등록 제10-0927834호Republic of Korea Patent Registration No. 10-0927834

본 발명은 혈당을 천천히 상승시켜 과도한 인슐린 분비를 예방할 수 있어 당뇨병 환자도 섭취할 수 있고, 노화 및 다양한 질병의 원인이 되는 활성산소종을 제거하는 항산화기능와 치매예방효과도 가지는 떡을 제공하는 것을 목적으로 한다.The present invention aims to provide a rice cake having an antioxidant function and a dementia-preventing effect that can increase the blood sugar slowly and prevent excessive insulin secretion, which can be consumed by diabetic patients and removes reactive oxygen species that cause aging and various diseases. Is done.

이를 위해 본 발명에서는 비트, 양파, 시금치를 사용하여 항당뇨효과와 항산화효과를 가지면서 동시에 색감이 예쁜 무지개떡을 제조하고자 한다. To this end, in the present invention, beet, onion, and spinach are used to produce a rainbow rice cake having an anti-diabetic effect and an antioxidant effect while having a beautiful color.

상기 목적을 달성하기 위하여, 본 발명은 비트, 양파 및 시금치를 세척하고 손질하는 단계; 상기 손질된 비트, 양파 및 시금치를 건조시키는 단계; 상기 건조된 비트, 양파 및 시금치를 각각 분쇄하고 체쳐서 비트분말, 양파분말 및 시금치분말을 제조하는 단계; 3개의 용기에 각각 쌀가루 100중량부와 물 15~25중량부를 넣어 반죽하는 단계; 상기 각 용기에 설탕 14~16중량부와 비트분말 2~4중량부, 설탕 14~16중량부와 양파분말 5~10중량부, 설탕 14~16중량부와 시금치분말 7~12중량부를 각각 혼합하고 체치는 단계; 찜기에 상기 각 혼합물을 순서대로 쌓는 단계; 및 15~25분 동안 찌는 단계를 포함하는, 비트, 양파 및 시금치를 함유하는 무지개떡의 제조방법을 제공한다.In order to achieve the above object, the present invention comprises the steps of washing and grooming beet, onion and spinach; Drying the trimmed beets, onions and spinach; Crushing and sieving the dried beet, onion, and spinach, respectively, to prepare beet powder, onion powder, and spinach powder; Kneading by putting 100 parts by weight of rice flour and 15-25 parts by weight of water in each of the three containers; In each container, 14 to 16 parts by weight of sugar and 2 to 4 parts by weight of beet powder, 14 to 16 parts by weight of sugar and 5 to 10 parts by weight of onion powder, 14 to 16 parts by weight of sugar and 7 to 12 parts by weight of spinach powder, respectively And sieve steps; Stacking each mixture in order in a steamer; And it provides a method of manufacturing a rainbow rice cake containing beet, onion and spinach, including steaming for 15-25 minutes.

상기 방법은, 상기 찌는 단계 후 5~10분 동안 뜸들이는 단계를 더 포함할 수 있다.The method may further include a steaming step for 5-10 minutes after the steaming step.

또한 본 발명은 상기 방법으로 제조된 비트, 양파 및 시금치를 함유하는 무지개떡을 제공한다.In addition, the present invention provides a rainbow rice cake containing beet, onion and spinach prepared by the above method.

본 발명의 비트, 양파 및 시금치를 함유하는 무지개떡은 혈당을 천천히 상승시켜 과도한 인슐린 분비를 예방할 수 있으므로 당뇨병 환자의 간식으로 유용하게 이용될 수 있다. 또한, 본 발명의 떡은 항산화효과 및 뇌신경세포 사멸 억제효과를 가져 노화를 억제하고 치매를 예방할 수 있는 효과도 있다. Rainbow rice cake containing beet, onion and spinach of the present invention can be used as a snack for diabetics because it can prevent excessive insulin secretion by slowly raising blood sugar. In addition, the rice cake of the present invention has an antioxidant effect and an inhibitory effect on cranial nerve cell death, thereby suppressing aging and preventing dementia.

도 1은 일반 백설기와 본 발명에 따라 제조된 무지개떡의 사진이다.
도 2는 실시예 1의 무지개떡의 혈당상승 억제효과를 확인한 결과이다.
도 3은 실시예 2의 무지개떡의 혈당상승 억제효과를 확인한 결과이다.
도 4는 원료 추출물의 DPPH 라디칼 소거활성을 확인한 결과이다.
도 5는 실시예 1의 무지개떡의 DPPH 라디칼 소거활성을 확인한 결과이다.
도 6은 실시예 2의 무지개떡의 DPPH 라디칼 소거활성을 확인한 결과이다.
도 7은 본 발명의 무지개떡의 뇌신경세포 사멸억제효과를 확인한 결과이다.
도 8은 본 발명의 무지개떡의 NO 생성 억제효과를 확인한 결과이다.
1 is a photograph of a rainbow Baekteok prepared according to the present invention with a general Snow White.
2 is a result of confirming the effect of inhibiting blood sugar rise of the rainbow rice cake of Example 1.
3 is a result confirming the effect of inhibiting blood sugar rise of the rainbow rice cake of Example 2.
4 is a result confirming the DPPH radical scavenging activity of the raw material extract.
5 is a result of confirming the DPPH radical scavenging activity of the rainbow rice cake of Example 1.
6 is a result of confirming the DPPH radical scavenging activity of rainbow rice cake of Example 2.
7 is a result of confirming the brain nerve cell death inhibitory effect of the rainbow rice cake of the present invention.
8 is a result confirming the NO production inhibitory effect of the rainbow rice cake of the present invention.

본 발명의 비트, 양파 및 시금치를 함유하는 무지개떡의 제조방법은,Method of manufacturing a rainbow rice cake containing beet, onion and spinach of the present invention,

비트, 양파 및 시금치를 세척하고 손질하는 단계; 상기 손질된 비트, 양파 및 시금치를 건조시키는 단계; 상기 건조된 비트, 양파 및 시금치를 각각 분쇄하고 체쳐서 비트분말, 양파분말 및 시금치분말을 제조하는 단계; 3개의 용기에 각각 쌀가루 100중량부와 물 15~25중량부를 넣어 반죽하는 단계; 상기 각 용기에 설탕 14~16중량부와 비트분말 2~4중량부, 설탕 14~16중량부와 양파분말 5~10중량부, 설탕 14~16중량부와 시금치분말 7~12중량부를 각각 혼합하고 체치는 단계; 찜기에 상기 각 혼합물을 순서대로 쌓는 단계; 및 15~25분 동안 찌는 단계를 포함한다.Washing and grooming beets, onions and spinach; Drying the trimmed beets, onions and spinach; Crushing and sieving the dried beet, onion, and spinach, respectively, to prepare beet powder, onion powder, and spinach powder; Kneading by putting 100 parts by weight of rice flour and 15-25 parts by weight of water in each of the three containers; In each container, 14 to 16 parts by weight of sugar and 2 to 4 parts by weight of beet powder, 14 to 16 parts by weight of sugar and 5 to 10 parts by weight of onion powder, 14 to 16 parts by weight of sugar and 7 to 12 parts by weight of spinach powder, respectively And sieve steps; Stacking each mixture in order in a steamer; And steaming for 15-25 minutes.

이하 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.

먼저, 비트, 양파 및 시금치를 세척하고 손질한다.First, the beets, onions and spinach are washed and trimmed.

상기 손질된 비트, 양파 및 시금치를 건조시킨다. 손질된 비트, 양파 및 시금치를 각각 건조기 위아래로 공기가 통할 수 있도록 듬성듬성 넣어 쌓아 올리고, 60℃에서 2~3일 동안 매일 확인하면서 완전히 부서질 정도로 건조시키는 것이 바람직하다.Dry the trimmed beet, onion and spinach. It is desirable to dry trimmed beets, onions, and spinach to the top and bottom of the dryer, so that they can be aired up and down, and then piled up and dried at 60° C. for 2-3 days each day.

상기 건조된 비트, 양파 및 시금치를 각각 분쇄하고 체쳐서 비트분말, 양파분말 및 시금치분말을 제조한다. 채소에 거칠게 남아있는 줄기부분이나 이물질 제거를 위해 체치는 과정을 거치며, 시료를 체쳤을 때 떡의 식감이 더 부드러워진다.The dried beet, onion and spinach are respectively crushed and sieved to prepare beet powder, onion powder and spinach powder. In order to remove stems or foreign matter remaining rough on the vegetables, the process of sifting is performed. When the sample is sifted, the texture of the rice cake becomes softer.

3개의 용기에 각각 쌀가루 100중량부와 함께 물 15~25중량부를 넣는다. 이렇게 쌀가루에 물주기를 하여 가루가 흩어지지 않도록 한 후 체를 쳐서 입자감을 곱게 한다. 바람직하게는 체로 두 번 내린다. In each of the three containers, add 15 to 25 parts by weight of water along with 100 parts by weight of rice flour. Water the rice flour to prevent the powder from scattering, and then sieve to grind the grain. Preferably sifted twice.

상기 각 용기에 설탕 14~16중량부와 비트분말 2~4중량부, 설탕 14~16중량부와 양파분말 5~10중량부, 설탕 14~16중량부와 시금치분말 7~12중량부를 각각 혼합하고 다시 체를 친다. 이렇게 혼합한 후 체침으로써 쌀가루가 뭉치지 않고 입자감이 곱으면서 전체적으로 골고루 섞일 수 있다.In each container, 14 to 16 parts by weight of sugar and 2 to 4 parts by weight of beet powder, 14 to 16 parts by weight of sugar and 5 to 10 parts by weight of onion powder, 14 to 16 parts by weight of sugar and 7 to 12 parts by weight of spinach powder, respectively And sieve again. After mixing, the rice flour does not clump, and the grains are finely mixed, so that they can be mixed evenly.

찜기에 상기 각 혼합물을 순서대로 쌓는다. 한정되는 것은 아니나, 아래로부터 비트-양파-시금치의 순서로 3단이 되도록 쌓는 것이 색감이 우수하다. 각 단을 쌓을 때 각 단의 윗면을 평평하게 해준다.Each mixture is stacked in order in a steamer. It is not limited, but it is excellent in color when stacked in three steps in the order of bit-onion-spinach from the bottom. When stacking each level, level the top of each level.

끓는 물에 15~25분 동안 찐다. 찌기 전 윗면에 칼집을 넣는 것이 바람직하다. 찐 후 5~10분 정도 뜸을 들이는 것이 보다 바람직하다.Steam for 15 to 25 minutes in boiling water. It is desirable to put a sheath on top before steaming. It is more preferable to add moxibustion for 5-10 minutes after steaming.

이하 실시예를 통하여 본 발명을 보다 상세하게 설명한다. 이들 실시예는 본 발명을 예시하는 것으로 본 발명의 범위가 이에 한정되는 것은 아니다.The present invention will be described in more detail through the following examples. These examples illustrate the present invention, and the scope of the present invention is not limited thereto.

<< 실시예Example 1> 1>

비트, 양파 및 시금치를 세척하고 손질하였다. 손질한 비트, 양파 및 시금치를 각각 건조기 위아래로 공기가 통할 수 있도록 듬성듬성 넣고 쌓아 올리고, 60℃에서 2~3일 동안 매일 확인하면서 완전히 부서질 정도로 건조시켰다.Beets, onions and spinach were washed and trimmed. Trimmed beets, onions, and spinach were piled up and stacked to allow air to flow up and down in the dryer, respectively, and dried at 60° C. for 2 to 3 days each day.

건조된 비트, 양파 및 시금치를 분쇄하고, 체를 쳐서 채소에 거칠게 남아있는 줄기부분이나 이물질을 제거하였다.The dried beets, onions, and spinach were crushed, and sieved to remove stem parts or foreign matter remaining rough on the vegetables.

3개의 볼에 각각 멥쌀가루 100g과 미지근한 물 20g을 넣어 손으로 뭉쳤을 때 흩어지지 않을 정도로 한 후 체로 두 번 내려서 입자감을 곱게 하여 준다. Add 100 g of uncooked rice flour and 20 g of lukewarm water to each of the three balls so that they do not disperse when they are put together by hand, and then sift twice to sift the grain.

각 볼에 설탕 15g과 비트가루 3g, 설탕 15g과 양파가루 7g, 설탕 15g과 시금치가루 10g을 각각 넣고 다시 체친다. In each bowl, add 15 g of sugar and 3 g of beetroot powder, 15 g of sugar and 7 g of onion powder, 15 g of sugar and 10 g of spinach powder, and sieve again.

스테인리스 원형 틀에 아래로부터 비트-양파-시금치의 순으로 3단이 되도록 쌓았다. 이때 각 단의 윗면을 평평하게 해준다.The stainless steel round frame was stacked to be three steps in order of beet-onion-spinach from below. At this time, level the top of each stage.

윗면에 칼집을 넣고 끓는 물에 20분 동안 쪄준 후 불을 끄고 5분 동안 뜸을 들여 무지개떡을 완성하였다. 완성된 무지개떡을 도 1(우측)에 나타내었다.Put a sheath on the top side, boil it in boiling water for 20 minutes, turn off the fire, and sterilize for 5 minutes to complete the rainbow cake. The completed rainbow rice cake is shown in Fig. 1 (right).

<실시예 2><Example 2>

비트가루 4g, 양파가루 10g 및 시금치가루 12g을 사용한 것을 제외하고는 실시예 1과 동일하게 떡을 제조하였다.The rice cake was prepared in the same manner as in Example 1, except that 4 g of beet flour, 10 g of onion powder, and 12 g of spinach powder were used.

완성된 무지개떡을 도 1(우측)에 나타내었다.The completed rainbow rice cake is shown in Fig. 1 (right).

<비교예><Comparative Example>

비교예를 위하여 일반 백설기떡을 다음과 같이 제조하였다.For comparison, a general Baeksulgitteok was prepared as follows.

멥쌀가루 300g에 미지근한 물 60g을 넣어 손으로 뭉쳤을 때 흩어지지 않을 정도로 반죽하였다. 이 반죽에 설탕 45g을 섞고 체쳤다. 60g of lukewarm water was added to 300g of uncooked rice flour, and when kneaded by hand, it was kneaded so as not to scatter. Mix 45 grams of sugar with this dough and sieve.

체친 반죽을 스테인리스 원형 틀에 넣고 숟가락으로 윗면을 평평하게 해주었다.The sieve dough was placed in a stainless steel round mold and the top was flattened with a spoon.

윗면에 칼집을 넣고 끓는 물에 20분 동안 쪄준 후 불을 끄고 5분 동안 뜸을 들여 백설기를 완성하였다.Put a sheath on the top side, boil it in boiling water for 20 minutes, turn off the fire, and sterilize for 5 minutes to complete the snow white.

완성된 백설기를 도 1(왼쪽)에 나타내었다.The completed snow white is shown in FIG. 1 (left).

[[ 실험예Experimental Example ]]

통계처리 Statistics processing

하기 각 실험결과는 평균(mean)±표준편차(standard deviation, SD)로 표시하였고 실험군간 평균의 차이는 원웨이(one-way) ANOVA로 유의성을 확인한 후 던컨의 다중검정(Duncan's multiple range test)을 이용하여 사후 검정하였으며 P<0.05 수준에서 유의성의 여부를 검증하였다. 모든 통계 분석은 SPSS(statistical package for the social science) version 12.0 프로그램(SPSS Inc., Chicago, IL, USA)을 이용하여 분석하였다.Each of the following experimental results was expressed as mean±standard deviation (SD), and the difference between the groups was confirmed by one-way ANOVA and then Duncan's multiple range test. It was tested by using and tested for significance at P <0.05 level. All statistical analysis was performed using a statistical package for the social science (SPSS) version 12.0 program (SPSS Inc., Chicago, IL, USA).

<실험예 1><Experimental Example 1>

관능검사Sensory test

관능검사는 대학생 30명을 선정하여 본 실험의 목적과 평가방법에 대한 교육을 실시한 후 5점 척도법으로 실시하였다.The sensory test was conducted with a 5-point scale method after selecting 30 college students and conducting education on the purpose and evaluation method of this experiment.

관능검사는 실험시료로 실시예 1과 2의 무지개떡을 사용하고 비교를 위한 시료로 비교예의 백설기를 사용하여 총 3가지를 평가하는 설문조사 방식으로 실시하였다.The sensory test was carried out in a questionnaire method in which a rainbow rice cake of Examples 1 and 2 was used as an experimental sample, and a total of three were evaluated using the snow white group of the comparative example as a sample for comparison.

관능평가 항목은 전체적인 외관, 색상, 향미, 맛, 조직감, 종합적인 기호도에 대하여 매우 좋다(5점), 좋다(4점), 그저 그렇다(3점), 나쁘다(2점), 매우 나쁘다(1점)로 평가하였다.The sensory evaluation items are very good (5 points), good (4 points), just (3 points), bad (2 points), and very bad (1) for the overall appearance, color, flavor, taste, texture, and overall preference. Point).

관능검사에 참여한 지원자들은 설문지에 나이와 성별 등을 기록하고, 한 시료의 평가를 마칠 때마다 물로 입을 세척한 후 다음 시료를 평가하였다. Volunteers who participated in the sensory test recorded age and gender on the questionnaire, and each time the evaluation of one sample was completed, the mouth was washed with water and the next sample was evaluated.

그 결과를 하기 표 1에 나타내었다.The results are shown in Table 1 below.

시료sample 외관Exterior 색상color 향미Flavor flavor 조직감Organization 기호도Preference 비교예Comparative example 4.00±1.044.00±1.04 3.97±1.143.97±1.14 3.82±1.143.82±1.14 4.12±0.984.12±0.98 4.15±0.864.15±0.86 4.03±0.904.03±0.90 실시예 1Example 1 4.21±0.884.21±0.88 4.24±0.854.24±0.85 3.15±1.233.15±1.23 3.12±1.273.12±1.27 3.58±1.213.58±1.21 3.39±1.183.39±1.18 실시예 2Example 2 4.27±0.794.27±0.79 4.27±0.864.27±0.86 2.97±1.112.97±1.11 3.00±1.043.00±1.04 3.80±1.073.80±1.07 3.64±1.073.64±1.07

상기 표 1의 결과에서와 같이, 외관, 색상, 조직감 및 종합적 기호도에서 실시예 2가 우수하였다. As in the results of Table 1, Example 2 was excellent in appearance, color, texture, and overall preference.

<실험예 2><Experimental Example 2>

혈당상승 억제효과 확인Check the effect of suppressing blood sugar

본 발명의 무지개떡의 혈당상승 억제효과를 확인하기 위하여 다음과 같이 실험하였다.In order to confirm the effect of inhibiting blood sugar elevation of the rainbow rice cake of the present invention, experiments were conducted as follows.

실험은 10명의 피실험자를 대상으로 실시하였다. 10명의 피실험자는 실험 1일차에는 공복상태에서 비교예의 백설기를 섭취하고, 실험 2일차에는 공복상태에서 실시예 1의 떡을 섭취하며, 실험 3일차에는 공복상태에서 실시예 2의 떡을 섭취하였다.The experiment was conducted on 10 subjects. On the first day of the experiment, 10 subjects ingested the snow white of the comparative example in the fasting state, on the second day of the experiment, the rice cake of Example 1 in the fasting state, and on the third day of the experiment, the rice cake of Example 2 in the fasting state.

각 경우 떡을 섭취하기 전, 떡을 섭취하고 15분 후, 30분 후, 1시간 후, 1시간 30분 후 및 2시간 후 총 6번 혈당을 검사하였다. In each case, a total of six blood glucose levels were tested before eating rice cake, 15 minutes, 30 minutes, 1 hour, 1 hour 30 minutes, and 2 hours after eating rice cake.

비교예, 실시예 1 및 실시예 2의 떡을 섭취한 경우의 혈당변화를 하기 표 2에 나타내었다. 또한 비교예와 실시예 1의 떡을 섭취한 경우의 혈당변화를 도 2에 나타내고, 비교예와 실시예 2의 떡을 섭취한 경우의 혈당변화를 도 3에 나타내었다. Comparative Example, Example 1 and Example 2 are shown in Table 2 below the blood glucose change when the rice cake was ingested. In addition, the change in blood sugar in the case of ingesting the rice cake of Comparative Example and Example 1 is shown in FIG. 2, and the change in blood sugar in the case of ingesting the rice cake of Comparative Example and Example 2 is shown in FIG.

시료sample 공복spandrel 15분15 minutes 30분30 minutes 60분60 minutes 90분90 minutes 120분120 minutes 비교예Comparative example 97.3±4.997.3±4.9 150.5±5.8150.5±5.8 175.5±15.0175.5±15.0 138.3±6.1138.3±6.1 124.8±8.4124.8±8.4 104.3±5.1104.3±5.1 실시예 1Example 1 94.25±5.594.25±5.5 130.25±5.1130.25±5.1 159.5±6.3159.5±6.3 143.25±11.0143.25±11.0 112.5±18.8112.5±18.8 102.5±7.2102.5±7.2 실시예 2Example 2 93.0±10.793.0±10.7 122.8±6.4122.8±6.4 139.5±6.1139.5±6.1 116.8±10.7116.8±10.7 99.5±4.799.5±4.7 84.0±6.384.0±6.3

이들 결과에서 확인되는 바와 같이, 실시예 1의 무지개떡을 섭취한 경우에는 비교예의 떡을 섭취한 경우에 비해 혈당상승을 억제하는 효과가 크지 않았지만, 실시예 2의 떡을 섭취한 경우에는 비교예의 떡을 섭취한 경우에 비해 혈당상승을 현저하게 억제하였다.As can be seen from these results, when the rainbow rice cake of Example 1 was ingested, the effect of suppressing blood sugar elevation was not as large as that of the rice cake of the comparative example, but when the rice cake of Example 2 was ingested, Compared to the intake of rice cake, blood sugar elevation was significantly suppressed.

<실험예 3><Experimental Example 3>

원료 추출물의 항산화활성(DPPH 라디칼 소거활성) 확인Confirmation of antioxidant activity (DPPH radical scavenging activity) of raw material extract

본 발명의 무지개떡에 사용되는 원료인 비트, 양파 및 시금치의 항산화효과를 확인하기 위하여 DPPH 라디칼 소거활성을 다음과 같이 확인하였다.DPPH radical scavenging activity was confirmed as follows to confirm the antioxidant effect of beet, onion, and spinach, which are raw materials used in the rainbow rice cake of the present invention.

1. 추출물 시료의 제조1. Preparation of extract samples

비트, 양파 및 시금치를 건조시켜 분쇄하여 분말을 제조하였다. 각 분말시료 1g당 10㎖의 70% 에탄올을 가하여 40℃에서 유효성분을 추출한 후 강압농축하여 동결건조한 것을 시료로 사용하였다.Beet, onion and spinach were dried and ground to prepare a powder. After adding 10 ml of 70% ethanol per 1 g of each powder sample, the active ingredient was extracted at 40° C., and then concentrated and subjected to lyophilization as a sample.

2. DPPH 라디칼 소거활성 확인2. Confirmation of DPPH radical scavenging activity

시료로는 상기 제조한 비트, 양파 및 시금치 추출물 시료를 0.1mg/㎖, 0.25mg/㎖. 0.5mg/㎖, 1mg/㎖, 2.5mg/㎖, 5mg/㎖ 및 10mg/㎖ 농도로 사용하였다.As a sample, 0.1 mg/ml, 0.25 mg/ml of the prepared beet, onion, and spinach extract samples. 0.5 mg/ml, 1 mg/ml, 2.5 mg/ml, 5 mg/ml and 10 mg/ml concentrations were used.

DPPH 라디칼 소거능은 각 농도 시료 1.8㎖와 DPPH(1,1-diphenyl-2picrylhydrazyl)용액(1.5×10-4M) 1.2㎖를 잘 혼합하고 37℃에서 30분 동안 반응시킨 후 517nm에서 흡광도를 측정하였다. DPPH 라디칼 소거 활성은 대조군에 대한 시료 첨가구의 흡광도를 비교하여 다음의 식으로 계산하였고 그 결과를 하기 표 3과 도 4에 나타내었다:The DPPH radical scavenging ability was well mixed with 1.8 ml of each concentration sample and 1.2 ml of DPPH (1,1-diphenyl-2picrylhydrazyl) solution (1.5×10 -4 M), reacted at 37°C for 30 minutes, and absorbance was measured at 517 nm. . DPPH radical scavenging activity was calculated by comparing the absorbance of the sample addition port with respect to the control group, and the results are shown in Tables 3 and 4 below:

DPPH 라디칼 소거활성(%) = [1-(시료의 흡광도/대조구의 흡광도)]×100DPPH radical scavenging activity (%) = [1-(absorbance of sample/absorbance of control)]×100

농도(mg/㎖)Concentration (mg/ml) 양파onion 시금치spinach 비트beat 1010 98.6±0.298.6±0.2 96.7±1.196.7±1.1 100±1.7100±1.7 55 98.1±0.598.1±0.5 95.3±1.995.3±1.9 98.9±7.398.9±7.3 2.52.5 93.5±4.393.5±4.3 94.7±094.7±0 97.9±2.697.9±2.6 1One 83.0±1.383.0±1.3 94.0±0.494.0±0.4 97.6±2.297.6±2.2 0.50.5 73.2±5.673.2±5.6 76.6±0.376.6±0.3 96.6±1.996.6±1.9 0.250.25 63.7±7.363.7±7.3 44.1±2.644.1±2.6 96.8±1.896.8±1.8 0.10.1 43.9±6.243.9±6.2 24.1±0.424.1±0.4 70.4±0.470.4±0.4

상기 결과에서와 같이, 본 발명에 사용된 원료인 비트, 양파 및 시금치는 모두 강한 항산화효과가 있었고 농도의존적으로 항산화효과가 증가하였다. 또한 비트, 양파 및 시금치 중 비트의 DPPH 라디칼 소거능이 양파나 시금치보다 높았고, 0.1mg/㎖와 0.5mg/㎖의 저농도에서는 양파의 DPPH 라디칼 소거능이 시금치보다 높았다.As in the above results, the raw materials used in the present invention, beet, onion and spinach all had strong antioxidant effects and concentration-dependent antioxidant effects increased. In addition, the DPPH radical scavenging ability of beet, onion and spinach was higher than that of onion or spinach, and at low concentrations of 0.1mg/ml and 0.5mg/ml, the DPPH radical scavenging ability of onion was higher than that of spinach.

<실험예 4><Experimental Example 4>

무지개떡의 항산화활성(DPPH 라디칼 소거활성) 확인Confirmation of antioxidant activity (DPPH radical scavenging activity) of rainbow rice cake

본 발명의 무지개떡의 항산화효과를 확인하기 위하여 DPPH 라디칼 소거활성을 다음과 같이 확인하였다.In order to confirm the antioxidant effect of the rainbow rice cake of the present invention, DPPH radical scavenging activity was confirmed as follows.

1. 추출물 시료의 제조1. Preparation of extract samples

실험시료로 실시예 1과 2의 무지개떡을 사용하고 비교를 위한 시료로 비교예의 백설기를 사용하였다. 또한 실시예 1과 2의 무지개떡에서 비트층, 양파층 및 시금치층을 각각 따로 분리하여 비교를 위한 시료로 사용하였다.Rainbow rice cakes of Examples 1 and 2 were used as an experimental sample, and a snow white group of a comparative example was used as a sample for comparison. In addition, in the rainbow rice cakes of Examples 1 and 2, the beet layer, the onion layer, and the spinach layer were separately separated and used as samples for comparison.

각각의 시료를 건조시킨 후 분쇄하여 분말을 제조하고, 각 분말시료 4g당 40㎖의 70% 에탄올을 가하여 40℃에서 쉐이킹(shaking) 하면서 유효성분을 추출한 후 감압 농축하여 동결 건조한 것을 시료로 사용하였다.Each sample was dried and then pulverized to prepare a powder, and 40 ml of 70% ethanol per 4 g of each powder sample was added to extract the active ingredient while shaking at 40° C., then concentrated under reduced pressure to use a freeze-dried sample. .

2. 2. DPPHDPPH 라디칼 소거활성 확인 Confirmation of radical scavenging activity

시료로는 상기 제조한 실시예 1, 실시예 2 및 비교예의 추출물 시료를 0.1mg/㎖, 0.25mg/㎖. 0.5mg/㎖, 1mg/㎖, 2.5mg/㎖, 5mg/㎖ 및 10mg/㎖ 농도로 사용하였다. 또한 실시예 1과 2의 무지개떡에서 분리한 비트층, 양파층 및 시금치층 추출물 시료도 동일한 농도로 사용하였다.As a sample, 0.1 mg/ml and 0.25 mg/ml of the extract samples of Examples 1, 2 and Comparative Examples prepared above. 0.5 mg/ml, 1 mg/ml, 2.5 mg/ml, 5 mg/ml and 10 mg/ml concentrations were used. In addition, samples of beet layer, onion layer and spinach layer separated from rainbow rice cakes of Examples 1 and 2 were also used at the same concentration.

DPPH 라디칼 소거능은 상기 실험예 3과 동일하게 확인하였다.DPPH radical scavenging ability was confirmed in the same manner as in Experimental Example 3.

실시예 1의 무지개떡과 비교를 위한 비교예의 백설기, 실시예 1의 무지개떡의 비트층, 양파층 및 시금치층에 대한 결과를 하기 표 4와 도 5에 나타내었다.Table 4 and FIG. 5 show the results of the snow white of the comparative example for comparison with the rainbow rice cake of Example 1, and the beet layer, onion layer and spinach layer of the rainbow rice cake of Example 1.

농도(mg/㎖)Concentration (mg/ml) 비교예Comparative example 실시예 1Example 1 양파층Onion layer 시금치층Spinach 비트층Bit layer 1010 39.3±439.3±4 91.0±0.391.0±0.3 92.4±0.192.4±0.1 94.3±2.294.3±2.2 89.8±0.689.8±0.6 55 20.7±1.720.7±1.7 75.8±3.275.8±3.2 64.5±3.864.5±3.8 87.1±3.887.1±3.8 83.5±0.683.5±0.6 2.52.5 10.8±0.810.8±0.8 45.8±6.145.8±6.1 42.9±6.542.9±6.5 49.8±8.849.8±8.8 48.4±8.748.4±8.7 1One 5.2±1.75.2±1.7 30.8±5.530.8±5.5 38.2±6.338.2±6.3 30.4±7.230.4±7.2 21.9±1.221.9±1.2 0.50.5 2.6±0.22.6±0.2 10.2±2.910.2±2.9 12.7±1.612.7±1.6 14.2±0.714.2±0.7 8.4±0.38.4±0.3 0.250.25 0±5.60±5.6 4.6±1.24.6±1.2 0±2.20±2.2 0±0.70±0.7 9.3±09.3±0 0.10.1 0±4.70±4.7 3.2±0.33.2±0.3 0.7±1.30.7±1.3 0±4.80±4.8 5.4±15.4±1

또한 실시예 1의 무지개떡과 비교를 위한 비교예의 백설기, 실시예 2의 무지개떡의 비트층, 양파층 및 시금치층에 대한 결과를 하기 표 5와 도 6에 나타내었다.In addition, the results of the snow white of the comparative example for comparison with the rainbow rice cake of Example 1, the beet layer, the onion layer and the spinach layer of the rainbow rice cake of Example 2 are shown in Tables 5 and 6 below.

농도(mg/㎖)Concentration (mg/ml) 비교예Comparative example 실시예 2Example 2 양파층Onion layer 시금치층Spinach 비트층Bit layer 1010 39.3±439.3±4 90.2±0.590.2±0.5 94.2±0.394.2±0.3 92.3±0.492.3±0.4 89.4±0.489.4±0.4 55 20.7±1.720.7±1.7 78.8±278.8±2 60.5±2.260.5±2.2 80.3±1.380.3±1.3 86.1±3.186.1±3.1 2.52.5 10.8±0.810.8±0.8 48.5±3.248.5±3.2 40.8±0.340.8±0.3 61.2±1.561.2±1.5 84.1±2.984.1±2.9 1One 5.2±1.75.2±1.7 27.8±1.127.8±1.1 20.4±0.920.4±0.9 40.4±3.840.4±3.8 42.0±1.442.0±1.4 0.50.5 2.6±0.22.6±0.2 14.4±1.514.4±1.5 15.0±0.315.0±0.3 12.8±2.212.8±2.2 37.0±2.837.0±2.8 0.250.25 0±5.60±5.6 11.1±0.611.1±0.6 7.5±17.5±1 15.5±0.715.5±0.7 20.4±0.920.4±0.9 0.10.1 0±4.70±4.7 7.1±0.87.1±0.8 3.5±0.13.5±0.1 10.2±1.310.2±1.3 12.3±212.3±2

상기 결과에서와 같이, 본 발명의 무지개떡은 비교예에 비하여 현저하게 높은 항산화효과를 나타내었고, 실시예 1의 무지개떡보다 실시예 2의 무지개떡이 더 강한 DPPH 라디칼 소거작용을 나타내었다. As in the above results, the rainbow rice cake of the present invention showed a significantly higher antioxidant effect than the comparative example, and the rainbow rice cake of Example 2 showed stronger DPPH radical scavenging activity than the rainbow rice cake of Example 1.

이러한 결과는 원료의 항산화효과가 무지개떡에 그대로 함유되어 있기 때문으로 실시예 2의 무지개떡이 실시예 1의 무지개떡보다 더 강한 항산화효과를 나타내는 것은 원료인 비트, 양파 및 시금치의 함량이 더 높기 때문인 것으로 생각된다. These results show that the antioxidant effect of the raw material is contained in the rainbow rice cake as it is, so that the rainbow rice cake of Example 2 exhibits a stronger antioxidant effect than the rainbow rice cake of Example 1, the higher the content of raw beet, onion, and spinach. I think it is because.

<실험예 5><Experimental Example 5>

뇌신경세포 사멸 억제 효과 확인Confirmation of the effect of suppressing neuronal cell death

1. 추출물 시료의 제조1. Preparation of extract samples

시료로 실시예 2의 무지개떡 을 사용하고 비교를 위한 시료로 비교예의 백설기를 사용하였다. The rainbow rice cake of Example 2 was used as a sample, and the snow white group of the comparative example was used as a sample for comparison.

각각의 시료를 건조시킨 후 분쇄하여 분말을 제조하고, 각 분말시료 4g당 40㎖의 70% 에탄올을 가하여 40℃에서 쉐이킹(shaking) 하면서 유효성분을 추출한 후 감압 농축하여 동결 건조한 것을 시료로 사용하였다.Each sample was dried and then pulverized to prepare a powder, and 40 ml of 70% ethanol per 4 g of each powder sample was added to extract the active ingredient while shaking at 40° C., then concentrated under reduced pressure to use a freeze-dried sample. .

비교를 위하여 실험예 3에서 제조한 비트, 양파 및 시금치 추출물 시료를 사용하였다.For comparison, beet, onion, and spinach extract samples prepared in Experimental Example 3 were used.

2. 세포 배양 및 처리2. Cell culture and treatment

사람의 신경모세포종 SK-N-SH를 10% 비활성화 우태아 혈청(fetal bovine serum, FBS; Gibco-BRL, Carlsbad, CA, USA)과 1% 페니실린(Gibco-BRL)과 스트렙토마이신(Gibco-BRL)을 넣은 EMEM(Eagle's Minimum Essential Medium; ATCC, Manassas, VA, USA) 배양액으로 5% CO2가 공급되는 배양기(Thermo Fisher Scientific, Waltham, MA, USA)에서 37℃ 조건으로 배양하였다.Human neuroblastoma SK-N-SH 10% inactivated fetal bovine serum (FBS; Gibco-BRL, Carlsbad, CA, USA) and 1% penicillin (Gibco-BRL) and streptomycin (Gibco-BRL) It was cultured at 37 °C in an incubator (Thermo Fisher Scientific, Waltham, MA, USA) in which 5% CO 2 is supplied as a culture medium containing EMEM (Eagle's Minimum Essential Medium; ATCC, Manassas, VA, USA).

충분한 세포를 얻기 위해 배양용 플라스크(T75 cm2)에서 세포를 배양하고, 2~3일에 한 번씩 배지를 80% 교체하여 충분히 자랐을 때 트립신-EDTA(ethylene diamine tetraacetic acid; Gibco-BRL) 용액(1×)을 사용하여 부착된 세포를 떼어 낸 후 성장배지에 세포수를 1×104 cells/well로 조정하여 24-웰 플레이트에 세포를 깔아주고 24시간 동안 배양하여 MTT 분석과 NO 측정에 사용하였다. In order to obtain sufficient cells, the cells were cultured in a culture flask (T75 cm2), and the medium was replaced with 80% of the medium once every 2-3 days, and when it grew sufficiently, trypsin-EDTA (ethylene diamine tetraacetic acid; Gibco-BRL) solution (1 After detaching the attached cells using x), the number of cells in the growth medium was adjusted to 1×10 4 cells/well, cells were laid on a 24-well plate, and cultured for 24 hours to be used for MTT analysis and NO measurement. .

2시간 기아상태를 유지한 후 시료를 우태아 혈청을 첨가하지 않은 성장배지로 각각 125㎍/㎖와 250㎍/㎖의 농도로 희석하여 24시간 단독 처리하여 시료의 세포독성을 알아보았다. NO 도너(donor)인 SNP(sodium nitroprusside) 처리에 의해 과다하게 증가된 NO에 대항하는 시료의 뇌신경세포 보호효과 및 NO 생성 억제능을 알아보기 위해 SK-N-SH 세포에 시료를 2시간 동안 처리한 후 1mM SNP를 24시간 SK-N-SH 세포에 처리하였다. 무처리군를 대조군으로 하였고 1mM SNP 단독 처리군을 양성 대조군으로 사용하였다.After maintaining the starvation condition for 2 hours, the sample was diluted with growth medium without fetal calf serum to a concentration of 125 µg/ml and 250 µg/ml, respectively, and treated alone for 24 hours to investigate cytotoxicity of the sample. The sample was treated with SK-N-SH cells for 2 hours to find out the protective effect of NO and the inhibition of NO production in samples against excessively increased NO by treatment with SNO (sodium nitroprusside), which is an NO donor. Thereafter, 1 mM SNP was treated with SK-N-SH cells for 24 hours. The untreated group was used as a control, and the 1 mM SNP-only treatment group was used as a positive control.

3. 뇌신경세포 사멸 억제효과 확인 3. Confirmation of the effect of suppressing neuronal cell death

배양이 끝난 SK-N-SH 세포의 생존율은 MTT[3-(4,5-dimethythiazol-2-yl)-2,5-dipheyl terazolium bromid] 환원 방법을 이용하여 측정하였다. 세포 생존율을 알아보기 위해 DPBS(Dulbecco's Phosphate-Buffered Saline, 1×)을 이용하여 5 mg/㎖ MTT 용액을 제조하여 150㎕/well 처리하였고, 4시간 다시 배양하였다. 배양 이후 배양액을 제거한 뒤 DMSO(dimethyl sulfoxide; Sigma-Aldrich Co., St. Louis, MO, USA)를 200㎕/well로 넣어 섞은 후 570nm에서 흡광도를 측정하였다. 흡광도 측정시 공시료는 DMSO로 하였고, 세포의 생존율은 아래와 같이 계산하였고, 그 결과를 도 7에 나타내었다:The survival rate of SK-N-SH cells after cultivation was measured using the MTT[3-(4,5-dimethythiazol-2-yl)-2,5-dipheyl terazolium bromid] reduction method. To determine the cell viability, 5 mg/ml MTT solution was prepared using DPBS (Dulbecco's Phosphate-Buffered Saline, 1×), treated with 150 μl/well, and incubated again for 4 hours. After incubation, the culture solution was removed, and then DMSO (dimethyl sulfoxide; Sigma-Aldrich Co., St. Louis, MO, USA) was added and mixed at 200 µl/well, and absorbance was measured at 570 nm. When measuring absorbance, the test sample was DMSO, and the cell viability was calculated as follows, and the results are shown in FIG. 7:

세포 생존율(%)=[시료 처리군의 흡광도/대조군의 흡광도]×100Cell viability (%) = [Absorbance of sample treatment group/absorbance of control group] × 100

도 7의 결과에서와 같이, NO 도너인 SNP를 뇌신경세포인 SK-N-SH 세포에 처리한 결과 세포 생존율이 71.7%로 29.3%의 세포가 사멸하였으나 비트, 양파, 시금치 및 이들을 사용하여 제조한 무지개떡을 처리하였을 때 세포 생존율이 증가하였다. 세포 생존율의 증가는 양파 처리군에서 가장 높았다.As in the results of FIG. 7, when the NO donor SNP was treated to SK-N-SH cells, which are brain neurons, the cell viability was 71.7% and 29.3% of the cells were killed, but beets, onions, spinach, and these were prepared. Cell viability increased when treated with Rainbow Rice Cake. The increase in cell viability was highest in the onion treatment group.

백설기 처리군은 세포 생존율에 영향을 미치지 못하였고 비트, 양파 및 시금치 분말을 이용하여 만든 무지개떡은 과량 분비된 NO에 대항하여 세포사멸 억제효과를 나타내었다.The snow white group had no effect on cell viability, and rainbow rice cake made with beet, onion, and spinach powder showed apoptosis inhibitory effect against excessive secreted NO.

이와 같은 결과로부터 비트, 양파 및 시금치를 이용하여 제조한 무지개떡을 섭취하면 알츠하이머와 같은 치매를 예방하고 치매가 진행되는 것을 억제할 수 있을 것이라고 생각된다. From these results, it is thought that if eating rainbow rice cake prepared using beet, onion, and spinach, dementia such as Alzheimer's can be prevented and progression of dementia can be suppressed.

4. NO 생성 억제효과 확인 4. Confirm NO inhibitory effect

배양액 내 NO 농도는 100㎕ 배양액과 동량의 100㎕ 그리스시약(griess regent)[A:B=1:1, A: 0.1% N-1-naphthyl ethylenediamine dihydrochloride(Sigma-Aldrich Co.) in H2O, B: 1% sulfanilamide(Sigma-Aldrich Co.) in 5% H3PO4]을 잘 혼합하였다. 빛을 차단한 상태로 실온에 10분 동안 방치한 후 540nm에서 흡광도를 측정하였다. 작성한 표준곡선으로 NO 함량을 측정하였고, 그 결과를 도 8에 나타내었다. The NO concentration in the culture medium is the same as 100 μl culture medium and 100 μl grease reagent (A:B=1:1, A: 0.1% N-1-naphthyl ethylenediamine dihydrochloride (Sigma-Aldrich Co.) in H 2 O , B: 1% sulfanilamide (Sigma-Aldrich Co.) in 5% H 3 PO 4 ] was well mixed. Absorbance was measured at 540 nm after standing at room temperature for 10 minutes while blocking light. The NO content was measured by the prepared standard curve, and the results are shown in FIG. 8.

도 8이 결과에서와 같이, NO 도너인 SNP를 뇌신경세포인 SK-N-SH 세포에 처리한 결과 NO가 현저히 증가한 것을 알 수 있었다. 고농도 비트, 양파, 시금치 및 이들을 사용한 무지개떡을 처리하였을 때은 NO 생성이 현저하게 억제되었다.8, as shown in the results, NO was significantly increased as a result of treating SNP, a donor, to SK-N-SH cells, which are brain neurons. When the high-concentration beet, onion, spinach, and rainbow rice cake using these were treated, NO production was significantly suppressed.

Claims (3)

비트, 양파 및 시금치를 세척하고 손질하는 단계;
상기 손질된 비트, 양파 및 시금치를 건조시키는 단계;
상기 건조된 비트, 양파 및 시금치를 각각 분쇄하고 체쳐서 비트분말, 양파분말 및 시금치분말을 제조하는 단계;
3개의 용기에 각각 쌀가루 100중량부와 물 15~25중량부를 넣어 반죽하는 단계;
상기 3개의 용기에 각각 설탕 14~16중량부와 비트분말 2~4중량부, 설탕 14~16중량부와 양파분말 5~10중량부, 설탕 14~16중량부와 시금치분말 7~12중량부를 혼합하고 체치는 단계;
찜기에 상기 각 혼합물을 순서대로 쌓는 단계; 및
15~25분 동안 찌는 단계를 포함하는, 비트, 양파 및 시금치를 함유하는 무지개떡의 제조방법.
Washing and grooming beets, onions and spinach;
Drying the trimmed beets, onions and spinach;
Crushing and sieving the dried beet, onion, and spinach, respectively, to prepare beet powder, onion powder, and spinach powder;
Kneading by putting 100 parts by weight of rice flour and 15-25 parts by weight of water in each of the three containers;
14 to 16 parts by weight of sugar and 2 to 4 parts by weight of beet powder, 14 to 16 parts by weight of sugar and 5 to 10 parts by weight of onion powder, 14 to 16 parts by weight of sugar and 7 to 12 parts by weight of spinach powder, respectively, in the three containers. Mixing and sifting;
Stacking each mixture in order in a steamer; And
Method of manufacturing a rainbow rice cake containing beet, onion and spinach, including steaming for 15-25 minutes.
제1항에 있어서,
상기 찌는 단계 후 5~10분 동안 뜸들이는 단계를 더 포함하는 것을 특징으로 하는 방법.
According to claim 1,
Method comprising the step of steaming for 5-10 minutes after the steaming step further.
제1항 또는 제2항의 방법으로 제조된 비트, 양파 및 시금치를 함유하는 무지개떡.A rainbow rice cake containing beet, onion and spinach prepared by the method of claim 1 or 2.
KR1020180155079A 2018-12-05 2018-12-05 Mujigae-tteok comprising beet, onion and spinach and producing method thereof KR20200068287A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020180155079A KR20200068287A (en) 2018-12-05 2018-12-05 Mujigae-tteok comprising beet, onion and spinach and producing method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020180155079A KR20200068287A (en) 2018-12-05 2018-12-05 Mujigae-tteok comprising beet, onion and spinach and producing method thereof

Publications (1)

Publication Number Publication Date
KR20200068287A true KR20200068287A (en) 2020-06-15

Family

ID=71081453

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020180155079A KR20200068287A (en) 2018-12-05 2018-12-05 Mujigae-tteok comprising beet, onion and spinach and producing method thereof

Country Status (1)

Country Link
KR (1) KR20200068287A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050075892A (en) 2004-01-16 2005-07-25 도명호 Method for cooking silkworm bread
KR20070074313A (en) 2006-01-09 2007-07-12 나창환 Cake powder for nature grace and incense and color
KR100927834B1 (en) 2008-04-11 2009-11-23 이해극 A method for producing color caramelized rice cake and a colored caramelized rice cake produced by the above method
KR20140016025A (en) 2012-07-30 2014-02-07 이경환 The manufacturing methods germinated brown knowledge rice paper cake
KR20150140899A (en) 2014-06-09 2015-12-17 김문국 the functional health bread product method of using onion syrup and salicornia powder

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050075892A (en) 2004-01-16 2005-07-25 도명호 Method for cooking silkworm bread
KR20070074313A (en) 2006-01-09 2007-07-12 나창환 Cake powder for nature grace and incense and color
KR100927834B1 (en) 2008-04-11 2009-11-23 이해극 A method for producing color caramelized rice cake and a colored caramelized rice cake produced by the above method
KR20140016025A (en) 2012-07-30 2014-02-07 이경환 The manufacturing methods germinated brown knowledge rice paper cake
KR20150140899A (en) 2014-06-09 2015-12-17 김문국 the functional health bread product method of using onion syrup and salicornia powder

Similar Documents

Publication Publication Date Title
CN105995993A (en) Food composition applicable to diabetic and preparation method thereof
CN110973613A (en) Plant composite powder composition suitable for weight-losing crowd, product suitable for weight-losing crowd and application
KR101039640B1 (en) Method for producing rice for reducing glucose using coating mixture of mulberry leaf extract and onion juice
KR20140046232A (en) Brown rice citron flakes and manufacturing method thereof
KR20150086984A (en) Method for producing cookie using Opunita humifusa powder and cookie produced by same method
KR20200068287A (en) Mujigae-tteok comprising beet, onion and spinach and producing method thereof
MEM Shehata et al. Nutritional, sensory evaluation and biological effect on diabetic rats of cakes enhanced with pumpkin fruit and its seeds
KR101887122B1 (en) Method for preparing functional noodle comprising auricularia auricula judae and functional noodle prepared thereof
KR101586053B1 (en) method manufacturing Powder of Cudrania tricuspidata
Ijarotimi et al. Evaluation of nutrient composition, glyceamic index and anti-diabetic potentials of multi-plant based functional foods in rats
KR102430026B1 (en) A process for the preparation of cicada larva cookie containing sprout barley ginseng and cicada larva cookie containing sprout barley ginseng prepared therefrom
KR100816422B1 (en) Functional rice coated with extrcts from root of curdrania tricuspidatadietary and the preparation method of thereof
KR101341966B1 (en) Improved taste of traditional Korean Snack containing GABA rice powder and process for preparation thereof
KR20210066246A (en) Manufacturing method of rice syrup comprising Muscat Bailey A
CN105581247A (en) Bitter gourd and tartary buckwheat noodles for patients with diabetes mellitus and cancers and preparation method of bitter gourd and tartary buckwheat noodles
KR20210093479A (en) Lectin-free K-dosa dough manufacturing method
KR102366453B1 (en) Composition comprising finger finger finger No. 1
RU2753976C1 (en) Method for obtaining grain bar
KR101578666B1 (en) Manufacturing method of Korea hot pepper paste pencake using sap of Acer mono
KR20150064521A (en) Fermented composition comprising bamboo salt and rhynchosia nulubilis
KR20200074355A (en) Method for Preparating functional grain syrup comprising Cynanchi wilfordii extract and functional grain syrup preparated thereby
KR890003738B1 (en) Process for making noodle mixing kale powder
Koubala et al. Effect of the various processed food of the Far North Cameroon on the glycemic index
KR101487245B1 (en) Yakgwa comprising red ginseng and Preparation method thereof
KR102658154B1 (en) Functional rice for dropping in blood sugar level

Legal Events

Date Code Title Description
E902 Notification of reason for refusal
AMND Amendment
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
X091 Application refused [patent]
AMND Amendment
E902 Notification of reason for refusal
X601 Decision of rejection after re-examination