KR20200064319A - Composition for skin anti-wrinkle comprising mixture of Ulmus davidiana var. japonica extract and Cornus officinalis extract as effective component - Google Patents

Abstract

The present invention relates to a composition for alleviating skin wrinkles containing a mixture of an Ulmus davidiana var. japonica extract and a Cornus officinalis extract as an active component. More specifically, the mixture of the Ulmus davidiana var. japonica extract and the Cornus officinalis extract is effective for alleviating wrinkles. Therefore, the composition of the present invention containing the Ulmus davidiana var. japonica extract and the Cornus officinalis extract can be usefully used as a material for functional cosmetics or health functional food for alleviating skin wrinkles.

Description

Composition for skin anti-wrinkle comprising mixture of Ulmus davidiana var. japonica extract and Cornus officinalis extract as effective component}

The present invention relates to a composition for improving skin wrinkles containing a mixture of elm and cornus officinalis as an active ingredient.

Skin aging refers to both the type and intangible changes that appear on the skin with age. Skin aging may be divided into endogenous physiological aging, which is naturally caused by an increase in physiological function of a living body as age increases, and exogenous photoaging, which is caused by long-term UV exposure. The clinical feature of endogenous skin aging is that the skin becomes thinner and the elasticity decreases. In addition, histological findings are that the thickness of the epidermis and dermis is thinned, blood vessels are reduced, and the number of fibroblasts in the dermis is reduced. In addition, the clinical characteristics of photoaging, which is exogenous skin aging, is that the skin becomes rough and elastic, irregular pigmentation occurs, and deep wrinkles increase.

Various causes of wrinkles have been reported. First, when the skin is exposed to excessive ultraviolet rays, collagen, which is a skin component, is accelerated, and thus the skin loses elasticity and wrinkles may be generated. In addition, if the skin becomes excessively dry due to excessive temperature change, low humidity, or wind, the skin function as a barrier to the outside is deteriorated and wrinkles may occur. In addition, when the skin is exposed to free radicals or free radicals, lipid peroxide is generated by oxidizing action, and thus, skin constituent proteins such as collagen are deformed and wrinkles may be generated. Various cosmetic compositions have been studied to suppress wrinkles caused by external factors and internal factors. In particular, in the case of functional cosmetics containing chemical components, there is a problem that it is not suitable for sensitive skin by causing skin irritation, and attention is focused on functional cosmetics derived from natural plants that have little irritation to the skin and are environmentally friendly.

On the other hand, as a prior art related to the wrinkle improvement effect, Korean Registered Patent No. 1574558 discloses a cosmetic composition for wrinkle improvement and moisturizing containing ginsenoside Rd, Rb2 and killing extract as an active ingredient, and Korean Patent Registration No. 1574248 Although a cosmetic composition for improving skin wrinkles containing an extract of Wang Ho Jang Jang as an active ingredient is disclosed, a composition for improving skin wrinkles containing a mixture of elm and cornus extract of the present invention as an active ingredient has not been disclosed.

The present invention was derived by the above-described needs, and provides a composition for improving skin wrinkles containing a mixture of elm and cornus extract as an active ingredient, wherein the mixture of elm extract and cornus extract is elm extract or cornus By confirming that the wrinkle improvement effect is enhanced compared to the extract alone, the present invention was completed.

In order to solve the above problems, the present invention provides a cosmetic composition for improving skin wrinkles containing a mixture of elm and cornus officinalis as an active ingredient.

In addition, the present invention provides a health functional food composition for improving skin wrinkles containing a mixture of elm and cornus extract as an active ingredient.

The present invention relates to a composition for improving skin wrinkles containing a mixture of elm and cornus extract as an active ingredient, wherein a mixture of elm and cornus extract extract inhibits collagenase activity compared to elm extract or cornus extract individual substances Since the effect is remarkable, the composition of the present invention containing a mixture of elm and cornus officinalis extracts as an active ingredient may be used as a cosmetic composition for improving skin wrinkles or a health functional food composition for improving skin wrinkles.

1 is a result confirming the cytotoxicity of elm extract or cornus extract. Control is an extract-free group.
2 is a result confirming the inhibitory effect of the collagen decomposition enzyme (collagenase) activity of elm extract or cornus extract. Control is a negative control treated only with collagenase extracted from Clostridium histolyticum , MI is Clostridium histolyticum ) is a positive control treated with collagenase and metalloprotease inhibitor.

The present invention relates to a cosmetic composition for improving skin wrinkles containing a mixture of elm and cornus officinalis extracts as an active ingredient.

In one embodiment of the present invention, the cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders , Oil, powder foundation, emulsion foundation, wax foundation and spray, etc., and more specifically, skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage Cream, nourishing cream, eye cream, moisturizing cream, hand cream, essence, nutrition essence, pack, cleansing foam, cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap and powder can be prepared in cosmetic formulations, It is not limited to this.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. This can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene Glycols, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, and the like.

The cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, for example, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, blowing agents ( foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives , Conventional adjuvants such as lipid vesicles, and carriers.

The present invention relates to a health functional food composition for improving skin wrinkles containing a mixture of elm and cornus officinalis extracts as an active ingredient.

The health functional food composition may be prepared in the form of powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient can be suitably used depending on the purpose of its use. In general, the health functional food composition of the present invention in the manufacture of food or beverage is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, when ingested for a long period of time for health purposes, the amount may be below the above range, and may be used in an amount above the above range within a range where there is no problem in terms of safety.

There are no particular restrictions on the type of the health functional food. Examples of foods to which the health functional food composition can be added are meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum products, dairy products including ice cream, various soups, beverages, tea Drinks, alcoholic beverages, and vitamin complexes are included, and all foods in the ordinary sense are included.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients that are conventionally added during food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink agent, natural carbohydrates or flavoring agents may be included as additional components in addition to the active ingredient. The natural carbohydrates include monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g. dextrins, cyclodextrins, etc.) or sugar alcohols (e.g. , Xylitol, sorbitol, erythritol, and the like). The flavoring agent may be a natural flavoring agent (eg, tau martin, stevia extract, etc.) and a synthetic flavoring agent (eg, saccharin, aspartame, etc.).

Various nutritional supplements, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid, in addition to the above-mentioned health functional food composition It may further contain a carbonation agent used in the beverage.

Hereinafter, the present invention will be described in more detail using examples. It is obvious to those skilled in the art that these examples are only intended to illustrate the present invention in more detail and that the scope of the present invention is not limited by them.

Example 1. Cytotoxicity of elm extract and Cornus officinalis extract

MTS(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) for the stability of elm extract and cornus extract samples -2H-tetrazolium, promega, USA) was analyzed to measure the survival rate of 293T cells, human kidney epithelial cells.

Cells were divided into 4x10 ³ cells/well in a 96-well plate, and then treated with samples according to concentration (10, 50, 100 µg/ml), and cultured at 37°C and 5% CO ₂ for 48 hours. After incubation, 20 μl of the MTS solution diluted in PBS at a concentration of 2 mg/ml was added, and cultured for 4 hours, and absorbance was measured at 490 nm with a microplate reader. Cell viability was calculated according to the following equation.

[Equation 1]

Cell viability (%) = absorbance of the sample-added group/absorbance of the control group x 100

As a result, it was confirmed that the elm extract and cornus officinalis extract are not cytotoxic, as disclosed in FIG. 1.

Example 2. Inhibition effect of collagenase activity of elm extract and Cornus officinalis extract

An EnzChek collagenase analysis kit (Molecular Probes Inc.) was used to confirm the inhibitory effect of elm extract and cornus officinalis on collagenase activity. Clostridium Histolyticum ) has the effect of inhibiting the activity of collagen degrading enzyme extracted from enzymes, substrates and concentrations of elm extract, cornus officinalis extract or mixtures thereof for 1 to 2 hours, followed by fluorescence plate reader ( Ex/Em :495/515nm) It was confirmed by measuring the absorbance. The enzyme-only treatment group was used as a control.

As a result, as shown in FIG. 2, the elm extract had a concentration-dependent inhibitory effect on collagenase activity at 10, 50, and 100 μg/ml, and the cornus extract had almost no inhibitory effect on collagenase activity at 10 μg/ml. However, when treated with 50 and 100㎍ / ㎖ it was confirmed that the concentration-dependent inhibitory effect of collagenase activity.

Claims (2)

A cosmetic composition for improving skin wrinkles containing a mixture of elm and cornus officinalis as an active ingredient. A health functional food composition for improving skin wrinkles containing a mixture of elm and cornus officinalis as an active ingredient.

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