KR20200049437A - Composition for increase of muscular functions or exercise capacity comprising Vigna unguiculata (L.) Walp., Vicia faba L., or Dolichos lablab L. - Google Patents

Abstract

The present invention relates to a composition for preventing or treating muscular diseases containing an extract of Vigna unguiculata, Vicia faba, or Lablab purpureus as an active component. The Vigna unguiculata, Vicia faba, or Lablab purpureus increases the activity of mTOR, which is a major biomarker involved in muscular function improvement, to increase the muscle mass, thereby being able to be used to prevent or treat muscular diseases. In addition, the Vigna unguiculata, Vicia faba, or Lablab purpureus increases the activity of PGC-1α, which is a major biomarker involved in exercise performance ability, to have an effect of significantly improve exercise performance ability, thereby being able to be usefully used as the composition for preventing, alleviating or treating muscular diseases, improving muscular functions, and improving exercise performance ability.

Description

Composition for increase of muscular functions or exercise capacity comprising Vigna unguiculata (L.) Walp., Vicia faba L., or Dolichos lablab L.}

The present invention relates to a composition for the prevention or treatment of muscle diseases comprising the eastern, persimmon or violet extract as an active ingredient. In addition, the present invention relates to a composition for improving muscle function and a composition for improving exercise performance, comprising the extract of eastern, jamdu or swallow bean as an active ingredient.

In the past 50-100 years, it has been known that a decrease in human physical activity is associated with an increased incidence of metabolic diseases such as type 2 diabetes, obesity, and cardiovascular disease. Lack of physical activity ranks fourth among the causes of death reported by the World Health Organization. Due to these phenomena, organizations such as the World Health Organization, the American Heart Association, and the British Heart Foundation recommend at least 30 minutes of aerobic exercise for at least 5 days a week. In fact, exercise reduced the incidence of diabetes, obesity, breast cancer and colorectal cancer, and showed good treatment effects for depression (Am. J. Cardiol. 110: 58B68B, 2012, Br. J. Pharmacol. 170: 1153-1166, 2013).

One of the representative methods for improving energy performance by promoting energy consumption is a method of generating ATP energy by increasing fatty acid oxidation by mitochondria. It has been found that the number and ability of mitochondria responsible for this is regulated by the peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) coactivator, and that PGC-1α activity is regulated by SIRT1 (sirtulin) (Cell Metab. 1: 361-370, 2005, EMBO. J. 26: 1913-1923, 2007).

Scarpulla and his collaborators identified a protein called nuclear respiratory factor (NRF) (J. Cell. Biochem. 97: 673-683, 2006). It has been found that proteins in the NRF group bind to the promoters of various genes of mitochondria in the nucleus to activate mitochondrial replication and transcription, thereby inducing mitochondrial production. Proteins of the NRF group were found to improve expression by physically interacting with the PGC-1α coactivator (Cell 98: 115-24, 1999). In addition, AMPK, pAMPK, PPARδ, ERRα, and Tfam are known as factors related to improvement in athletic performance.

On the other hand, muscle atrophy (Muscle atrophy) is caused by a gradual decrease in muscle mass, and refers to muscle weakness and degeneration (Cell 119: 907-910, 2004). Muscle atrophy is promoted by inactivity, oxidative stress, and chronic inflammation and weakens muscle function and motor capacity (Clin. Nutr. 26: 524-534, 2007). The most important factor in determining muscle function is muscle mass, which is maintained by a balance of protein synthesis and degradation. Muscular dystrophy occurs when protein degradation occurs more than synthesis (Cell Biol. 37: 1985-1996, 2005).

Muscle size is regulated by intracellular signaling pathways that induce anabolism or catabolism within the muscle, and when there are more signaling reactions that induce synthesis than degradation of muscle proteins Muscle protein synthesis is increased, which is indicated by an increase in muscle size (hypertrophy) or an increase in muscle fiber number (hyperplasia) according to an increase in muscle protein (The Korea J. Sports Sci. 30: 1551-1561, 2011).

Muscle hypertrophy-inducing factors induce protein synthesis by phosphorylating downstream proteins from the stimulation of phosphatidylinositol-3 kinase (PI3K) / Akt pathway in muscle cells. Among them, the activity of mammalian target of rapamycin (mTOR) by PI3K / Akt signaling is recognized as a central growth signaling mechanism that integrates various growth signals in cells. Activation of mTOR contributes to muscle mass increase by inducing muscle protein synthesis by activating two downstream targets, 4E-binding protein (4E-BP1) and phosphorylated 70-kDa ribosomal S6 kinase (p70S6K) (J Biol. Chem. 278: 40717-40722, 2003, The Korea J. Sports Sci. 30: 1551-1561, 2011).

The eastern ( Vigna unguiculata (L.) Walp.), Vicia faba L., or Dolichos lablab L. is a plant in the family of Fabaceae that has 19-26% protein and various bioactivity in seeds. It contains. It is classified as a protein source food due to its high protein content (Korean J. Food Culture 28: 409-414, 2013). Physiological activities revealed to date include antioxidant (Food Chem. 101: 10-19, 2007), anti-inflammatory (Food Chem. 138: 1543-1550, 2013), and hyperlipidemia improvement (Korean J. Food Culture 28: 409-414, 2013 ; Sci. Food Agric. 95: 1207-1216, 2015), etc., but prior to the present invention, there is no known effect on the improvement of muscle function or exercise performance of eastern, jamdu, or swallow beans.

Accordingly, the present inventors conducted a study using various natural materials to develop a composition capable of improving muscle function or athletic ability, and as a result, Dongbu, Jamdu, or Swallow Soybean extract had muscle-generating activity and activity-enhancing activity Confirmed to complete the present invention.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing or treating muscle disease comprising any extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In addition, another object of the present invention is to provide a health functional food composition for preventing or improving muscle disease comprising any one extract selected from the group consisting of eastern, jamdu and swallow beans.

In addition, another object of the present invention to provide a food composition for improving muscle function comprising any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In addition, another object of the present invention is to provide a pharmaceutical composition for improving exercise performance, including any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In addition, another object of the present invention is to provide a food composition for improving exercise performance, including any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating muscle disease comprising any extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a health functional food composition for preventing or improving muscle disease comprising any extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a food composition for improving muscle function comprising any extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a pharmaceutical composition for improving exercise performance, including any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a food composition for improving exercise performance, including any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for the prevention or treatment of muscle diseases comprising any extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient.

At this time, the seeds, leaves, stems, flowers, roots, husks, fruits or mixtures of the above eastern ( Vigna unguiculata (L.) Walp.), Vicia faba L. or Swallow beans ( Dolichos lablab L.) are extracted It can be a target.

The pharmaceutical composition according to the present invention may be a composition containing any one extract selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient, and any one selected from the group consisting of eastern, jamdu and swallow beans as an active ingredient It may be a composition consisting of an extract of, or may be a composition consisting essentially of any one extract selected from the group consisting of eastern, jamdu and swallow beans as active ingredients.

In the present specification, the term 'comprising' is used in the same sense as 'including' or 'characterized by', and in the composition or method according to the present invention, specifically It does not exclude additional component or method steps not mentioned. In addition, the term 'consisting of' means excluding additional elements, steps or ingredients not separately described. The term 'essentially consisting of' means that in the scope of the composition or method, it may include substances or steps that do not materially affect the basic properties thereof, in addition to the substances or steps described. .

The eastern, jamdu or swallow beans belong to soybeans, and the components of soybeans include proteins, fats, carbohydrates, vitamins B1, B2, daidzin, genistin, and glucose. On the other hand, in the production of muscle, the supply of essential amino acids is very important. However, in the case of soybean, it is a limiting amino acid that lacks amino acids, including sulfur, such as methionine and cystine, and it is difficult to build muscle by eating only soybeans (J. Agric. Food Chem., 2001, 49 (3), pp 1069-1086).

The eastern part of the present invention ( Vigna unguiculata (L.) Walp.) Is a perennial plant belonging to the Rosaceae family and is called 'Cow pea' in the English-speaking region, and 'Gang-du' and 'Jang-du' in Asia and Korea. It is called ',' Eastern ',' Dombu ',' Donbu ',' Dombi ',' Gwangjeogi ',' Gwangjeongi ',' Juldombi ', etc., and is cultivated in warm regions. Dongbu is also used as a medicine in oriental medicine. It is known to protect the kidneys, strengthen the stomach, promote blood circulation, and is effective for diabetes, vomiting, and diarrhea. In addition, it contains high amounts of vitamins B1 and B2 and has a high lysine content, which prevents fatigue accumulation and helps collagen production, growth and development. In addition, the polyphenol content is higher than that of red beans, and it has anti-cancer effects, prevents aging, and helps circulation.

Jam beans ( Vicia faba L.) of the present invention are called by various names such as silkworm beans, small peas, and soybeans, and while having strong resistance to cold among the beans, they are very weak crops during drought and require high temperature and high humidity conditions after flowering. . Jamdu is rich in protein and dietary fiber, low in fat and highly nutritious food with vitamins A, B1, B2, C and various minerals. It is also a good source of vitamin B1 and thiamine, iron, copper, phosphorus, calcium and magnesium. Vitamin B1 in jamdu plays an important role in nervous system function and energy metabolism, iron and copper help form red blood cells, and phosphorus and magnesium help maintain strong bones. In addition, Jamdudo helps relieve inflammation such as hemorrhoids, sinusitis, otitis media, and gastritis and has the effect of reducing cholesterol in the blood. In oriental medicine, it is also used for medicinal purposes. The mature seeds and leaves of Jamdu are used as geothermal, diuretic, and antidote.

Swallow bean ( Dolichos lablab L.) of the present invention is also called migraine, stool, small bean, blackcurrant, green bean, and is a tropical origin of South America and is an annual plant in Korea. 3-O- [α-L-lamnopyranosyl- (1 → 2) -β-D-galactopyranosyl (1 → 2) as a protein, fat and major component of triterpenoid saponin -β-D-glucuronopyranosyl ((1 →)]-22-O- [2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one ( 2 '→)]-3β, 22β, 24-trihydroxyolean-12-ene-28-ol (3-O- [α-L-rhamnopyranosyl- (1 → 2) -β-D-galactopyranosyl (1 → 2) -β-D-glucuronopyranosyl (1 →)]-22-O- [2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (2 '→)]- 3β, 22β24-trihydroxyolean-12-en-28-al), lablabosides A, B, C, D, E, F, etc. In addition, the phytohemagglutinin (phytohemagglutinin) Contains phytohemagglutinin (PHA), trigonelline, 3-O-β-D-glucopyranosyl gibberellin A (3-O-β-D-glucopyranosyl gibberellin A), and the like.

As a pharmacological action, antibacterial, anti-tumor, immune-enhancing, strong blood cell, red blood cell aggregation, and antitrypsin action are known, and it is known that prolonged primitive activity of hematopoietic cells can be used for stem cell transplantation in gene therapy.

As described above, it is known that Dongbu, Jamdu, or Swallow Beans have different effects because they have different properties and properties, and the main components included are different. However, it is not known about the effect of promoting muscle formation, improving muscle function, or improving exercise performance in eastern, jamdu, and swallow beans.

In the present invention, "muscle" refers to the tendons, muscles, tendons, and "muscle function" refers to the ability to exert power by contraction of muscles, and the muscles can exert maximum contractility to overcome resistance. Includes muscle strength, the ability to have strength, muscle endurance, the ability to indicate how long or how many times a muscle can repeat contraction and relaxation at a given weight, and wits, the ability to exert strong power in a short period of time. These muscle functions are subjective to the liver, proportional to muscle mass, and "improving muscle function" means improving muscle function better.

In the present invention, "exercise performance" means that when the physical motion seen in everyday life or sports is externally divided into running, running, throwing, swimming, etc., the motion can be quickly, strongly, accurately, long, and proficient. To indicate the degree to be exercised, exercise performance is defined as factors such as muscle strength, agility, and endurance, and "improving exercise performance" means improving or improving exercise performance.

Specifically, the eastern, jamdu or swallow bean extract may be extracted using water, an organic solvent having 1 to 4 carbon atoms, or a mixture thereof as a solvent. At this time, the organic solvent is C1 to C4 lower alcohol (alcohol), acetone (acetone), ether (ether), benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), One or more selected from the group consisting of hexane, cyclohexane and petroleum ether is preferred, but is not limited thereto.

In addition, the lower alcohol used for the extraction of the eastern, jamdu or swallow beans is also more preferably ethanol or methanol, and most preferably ethanol. It is preferable to extract water, alcohol, or a mixture thereof by adding 2 to 20 times the weight of the crushed eastern, jamdu or swallow beans after crushing the dried eastern, jamdu or swallow beans during extraction, and 3 to 10 times It is more preferable to extract by adding, but is not limited thereto. When hot water is extracted, the extraction temperature is preferably 30 ° C to 100 ° C, and more preferably 60 ° C to 100 ° C, but is not limited thereto. The extraction time is preferably 1 hour to 10 hours, more preferably 2 hours to 5 hours, but is not limited thereto. Cold extraction, ultrasonic extraction or reflux cooling extraction may be used as the extraction method, and it is preferable to use a reflux cooling extraction method, but is not limited thereto. The number of extractions is preferably 1 to 5 times, and it is more preferable to repeatedly extract 2 to 3 times, but is not limited thereto.

In addition, the eastern, jamdu or swallow bean extract may be performed by subcritical fluid or supercritical fluid extraction.

In addition, the extract may be extracted at very high pressure. Preferably, it may be an ultra-high pressure condition of 100 MPa to 1000 MPa, but is not limited thereto.

The eastern, jamdu or swallow bean extract of the present invention may include a pre-treatment process such as a drying process for the raw material in order to increase extraction efficiency in the manufacture of the extract.

The extract of the present invention can be used as a liquid by filtration and / or concentration, and can be used by solidifying through a common drying process such as spray drying or freeze drying. In the drying process, dextrin may be mixed and dried before spray drying or freeze drying.

The muscle disease of the present invention is preferably a disease reported in the art as a muscle disease caused by muscle dysfunction, muscle wasting, or muscle degeneration, specifically, dystonia, muscle atrophy, and muscular dystrophy , Muscle degeneration, myasthenia, cachexia (cachexia) and sarcopenia (sarcopenia) is more preferably any one or more selected from the group consisting of, but is not limited to this.

The muscle wasting or degeneration is caused by factors such as wholly factors, acquired factors, aging, etc., and muscle wasting is characterized by gradual loss of muscle mass, weakening and degeneration of muscles, especially skeletal or veterinary muscles and cardiac muscles.

The eastern, peanut or swallow bean extract of the present invention can be used for the prevention or treatment of muscle diseases caused by muscle function degradation, muscle wasting or muscle degeneration as muscle activity can be increased as the activity of mTOR is significantly increased in muscle cells. Can be. The muscle is not limited in its kind, and muscle growth is to improve muscle performance, especially among body components, which can increase muscle mass through physical exercise and endurance enhancement, and administer substances having muscle growth effects to the body You can increase your muscle mass in the same way.

'Treatment' as used herein refers to a clinical procedure to alter the natural course of the individual or cell being treated, and may also be performed for the prevention of clinical pathology. The desirable effects of treatment include suppressing the occurrence or recurrence of the disease, alleviating symptoms, reducing any direct or indirect pathological consequences of the disease, reducing the rate of disease progression, improving the condition of the disease, improving, alleviating, or improving prognosis. Includes. The term 'prevention' also means any action that suppresses the onset of disease or delays its progression.

The dose of the eastern, peanut or swallow bean extract is preferably 1 μg / ml to 100 μg / ml, but is not limited thereto. At this time, when the eastern, jamdu or swallow bean extract is less than the weight part, the mTOR activity in muscle cells is lowered, and there is a problem that it is difficult to exert a muscle disease prevention or treatment effect, and the eastern, jamdu or swallow bean extract has the weight part If exceeded, there may be toxicity concerns, including cytotoxicity.

Pharmaceutical compositions for the prevention or treatment of muscle diseases according to the present invention are in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injectable solutions, respectively, according to a conventional method. It can be formulated and used as a formulation, and may include a suitable carrier, excipient, or diluent commonly used in the manufacture of pharmaceutical compositions for formulation.

Examples of the carrier or excipient or diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, and crude And various compounds or mixtures including vaginal cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, it can be prepared using diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc., which are usually used.

The solid preparation for oral administration may be prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in the eastern, pea or swallow bean extract. In addition, lubricants such as magnesium stearate and talc may be used in addition to simple excipients.

The solid preparation for oral administration may be prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in the eastern, pea or swallow bean extract. In addition, lubricants such as magnesium stearate and talc may be used in addition to simple excipients.

Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used as diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, may be included. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous agents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerol gelatin can be used.

The preferred dosage of the pharmaceutical composition for preventing or treating muscle diseases according to the present invention varies depending on the patient's condition, body weight, degree of disease, drug form, administration route and duration, but can be appropriately selected by those skilled in the art. However, for a desired effect, it can be administered at 0.0001 to 2,000 mg / kg per day, preferably at 0.001 to 2,000 mg / kg per day. Administration may be administered once a day, or may be divided into several times. However, the scope of the present invention is not limited by the dosage.

The pharmaceutical composition for preventing or treating muscle diseases according to the present invention can be administered to various mammals, such as rats, mice, livestock, and humans. All modes of administration can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine epidural or intracerebroventricular injection.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormonal therapy, chemotherapy, or biological response modifiers.

When the pharmaceutical composition for preventing and treating muscle diseases of the present invention is used as an external preparation for skin, fat substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents (foaming) agent), fragrance, surfactant, water, ionic emulsifier, nonionic emulsifier, filler, metal ion blocker, chelating agent, preservative, vitamin, blocker, wetting agent, essential oil, dye, pigment, hydrophilic active agent, lipophilic It may contain adjuvants commonly used in the field of skin science, such as any other ingredients commonly used in skin external preparations such as active agents or lipid vesicles. In addition, the ingredients may be introduced in an amount commonly used in the field of skin science.

When the pharmaceutical composition for preventing or treating muscle diseases of the present invention is provided as an external preparation for skin, the present invention is not limited thereto, and may be a formulation such as ointment, patch, gel, cream or spray.

In addition, the present invention provides a health functional food composition for preventing or improving muscle disease comprising any one extract selected from the group consisting of eastern, jamdu and swallow beans.

When the composition for preventing or improving muscle disease of the present invention is a food composition, it may be used for preventing or improving muscle disease due to muscle wasting or degeneration. Muscle wasting and degeneration are caused by genetic factors, acquired factors, aging, and the like, and muscle wasting is characterized by gradual loss of muscle mass, weakness and degeneration of muscles, especially skeletal or veterinary muscles and cardiac muscles. Examples of related diseases include sarcopenia, atony, muscular atrophy, muscular dystrophy, muscle degeneration, muscle spasm, muscular dystrophy, muscular atrophy sclerosis, myasthenia and cachexia ) And the like. The composition of the present invention has an effect of increasing muscle mass, and the muscle does not limit its type.

The food composition of the present invention includes all forms of functional foods, nutritional supplements, health foods, food additives and feeds, and includes animals including humans or livestock. Subject to eating. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. Drinks (including alcoholic beverages), fruits and processed foods thereof (e.g. canned fruits, canned foods, jams, marmalades, etc.), fish, meat and processed foods (e.g. ham, sausages) Corn beef, etc.), breads and noodles (e.g. udon, buckwheat noodles, ramen, spagate, macaroni, etc.), juice, various drinks, cookies, syrup, dairy products (e.g. butter, cheese, etc.), edible vegetable oil, margarine , Vegetable protein, retort foods, frozen foods, various seasonings (eg, miso, soy sauce, sauce, etc.) can be prepared by adding the above extracts of eastern, green beans or soybeans. In addition, as a nutritional supplement, but not limited to, capsules, tablets, etc. can be prepared by adding eastern, jamdu or swallow bean extract. In addition, as a health functional food, but not limited to, for example, the eastern, jamdu or swallow bean extract itself is prepared in the form of tea, juice and drink to be liquefied, granulated, encapsulated and encapsulated for drinking (health drink). Powdered can be consumed. In addition, in order to use the eastern, red bean or swallow bean extract in the form of food additives, it can be prepared and used in powder or concentrate form. In addition, it can be prepared in the form of a composition by mixing it with a known active ingredient known to have an effect of preventing muscle disease and improving muscle function with the extract of eastern, red bean or swallow bean.

When the composition for preventing or improving muscle disease of the present invention is used as a health drink composition, the health drink composition may contain various flavoring agents or natural carbohydrates, etc., as additional ingredients, as in conventional beverages. The natural carbohydrates described above include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Polysaccharides such as dextrin and cyclodextrin; Sugar alcohols such as xylitol, sorbitol, and erythritol. Sweeteners include natural sweeteners such as taumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g per 100 mL of the composition of the present invention, preferably about 0.02 to 0.03 g.

When the eastern, peanut or swallow bean extract is contained as an active ingredient in a food composition for preventing or improving muscle disease, the amount is not particularly limited to an amount effective to achieve an action for preventing muscle disease and improving muscle function, but the entire composition It is preferably 0.01 to 100% by weight relative to the total weight. The food composition of the present invention can be prepared by mixing together with the extract of eastern, red bean or swallow bean along with other active ingredients known to be effective in the composition for preventing muscle disease and improving muscle function.

In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid, salts of pectic acids, alginic acids, salts of alginic acids, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, Glycerin, alcohol or carbonic acid. In addition, the health food of the present invention may contain flesh for the production of natural fruit juice, fruit juice beverage or vegetable beverage. These ingredients may be used independently or in combination. The proportion of these additives is not critical, but is generally selected from 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the present invention provides a food composition for improving muscle function comprising the extract of eastern, jamdu or swallow bean as an active ingredient.

Examples and contents of the food composition are as described above, and the effect of improving muscle function thereof is also as described above.

In addition, the present invention provides a pharmaceutical composition for improving exercise performance, including Dongbu, Jamdu or Swallow Bean extract as an active ingredient.

Examples and contents of the pharmaceutical composition are as described above. The composition may increase the oxidative metabolic function of mitochondria, and specifically, the eastern, jamdu or swallow bean extracts of the present invention significantly increase the activity of PCG-1α in muscle cells, thereby enhancing exercise performance. Can be.

When the composition of the present invention is a pharmaceutical composition for improving athletic performance, it can be used for prevention or treatment of diseases caused by deterioration of athletic performance. Examples of diseases related to this include degenerative diseases, mitochondrial abnormalities, endurance syndrome, wheezing, lethargy, muscle abolition and depression. The composition of the present invention has an effect of improving exercise performance, and does not limit the form and type of exercise.

In addition, the present invention provides a food composition for improving exercise performance, including Dongbu, Jamdu or Swallow Bean extract as an active ingredient.

The examples and contents of the food composition are as described above, and the term 'improvement of exercise performance' in the present specification means an effect of improving muscle function such as muscle strength and endurance, preferably muscle strength or endurance. Will increase.

Therefore, the composition has an effect of increasing exercise endurance, strengthening muscle strength, enhancing balance, and / or enhancing exercise adaptation.

The present invention relates to a composition for the prevention or treatment of muscle diseases comprising the extract of eastern, pea, or swallow bean as an active ingredient, wherein the eastern, pea, or swallow bean increases the activity of mTOR, a major biomarker involved in muscle function improvement. By increasing muscle mass, it can be used for the prevention or treatment of muscle diseases, and by increasing the activity of PCG-1α, a major biomarker involved in exercise performance, has the effect of improving exercise performance. have.

Figure 1 shows the results of measuring the activity of mTOR in L6 muscle cells, eastern, jamdu, or swallow bean ethanol extract treatment.
Figure 2 shows the results of measuring the activity of mTOR in L6 muscle cells, eastern, jamdu, or swallow bean hot water extract treatment.
Figure 3 shows the results of measuring the activity of mTOR in L6 muscle cells, eastern, jamdu, or swallow bean subcritical extract treatment.
Figure 4 shows the results of measuring the activity of PGC-1α according to ethanol extract treatment of eastern, jamdu, or swallow beans in COS-7 kidney cells.
Figure 5 shows the results of measuring the activity of PGC-1α according to the treatment of eastern, jamdu, or swallow bean hot water extract in COS-7 kidney cells.
Figure 6 shows the results of measuring the activity of PGC-1α according to the sub-critical extract of eastern, jamdu, or swallow beans in COS-7 kidney cells.

Hereinafter, preferred embodiments are provided to help understanding of the present invention. However, the following examples are only provided to more easily understand the present invention, and the contents of the present invention are not limited by the following examples.

[Example]

Example 1. Preparation of eastern extract

(1) Preparation of eastern methanol extract

The dried eastern part was pulverized with a mixer, and then 100 g of the crushed eastern sample was added to 1 L of 100% methanol and repeatedly extracted three times at room temperature for 24 hours. The extracted sample was filtered under reduced pressure with Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove the solvent component to prepare an eastern methanol extract.

(2) Preparation of eastern ethanol extract

It was performed in the same manner as in Example 1- (1), except that 100% ethanol was used, and as a result, an eastern ethanol extract was prepared.

(3) Preparation of eastern ethyl acetate extract

It was carried out in the same manner as in Example 1- (1), except that 100% ethyl acetate was used, and as a result, eastern ethyl acetate extract was prepared.

(4) Preparation of eastern hexane extract

It was performed in the same manner as in Example 1- (1), except that 100% hexane was used, and as a result, an eastern hexane extract was prepared.

(5) Preparation of eastern hydrothermal extract

The dried eastern part was pulverized with a mixer, and then 100 g of the crushed eastern sample was added to 1 L of water and extracted with stirring at 80 ° C for 2 hours. Then, it was performed in the same manner as in Example 1- (1), and as a result, an Eastern hot water extract was prepared.

(6) Preparation of eastern ultra high pressure extract

The dried eastern part was crushed with a mixer, and then 76 mL of 18% ethanol was placed in a polyethylene pack, sealed, and then extracted using an ultra high pressure extraction device (Frescal MFP-7000; Mitsubishi Heavy Industries, Tokyo, Japan). The extraction conditions of the ultra-high pressure were 320 MPa and the extraction time was 5 minutes. Subsequently, it was performed in the same manner as in Example 1- (1), and as a result, an eastern ultra-high pressure extract was prepared.

(7) Preparation of eastern subcritical extract

The dried eastern part was pulverized with a mixer, and 50 g of the crushed eastern part was placed in a subcritical water reactor of a subcritical extraction device (Biovan, Gyeonggi, Korea) together with 1 L water and sealed. After sealing, the temperature of the reactor was raised to 200 ° C, and when the temperature of the reactor reached 200 ° C, the temperature was maintained for 20 minutes to extract. After 20 minutes, the extract was transferred to a storage tank supplied with cooling water, rapidly cooled to 30 ° C, and centrifuged for 30 minutes at 3,600 rpm to separate the floating residue, and only the supernatant was taken. The eastern subcritical extract was prepared by removing all solvents using a freeze dryer (ilShin Lab Co. Ltd., Seoul, Korea).

Example 2. Preparation of extract of jamdu

(1) Preparation of Jamdu methanol extract

It was carried out in the same manner as in Example 1- (1), except that Jamdu was used, and as a result, a Jamdu methanol extract was prepared.

(2) Preparation of ethanol extract from Jamdu

It was carried out in the same manner as in Example 1- (2), except that Jamdu was used, and as a result, an extract of Jamdu ethanol was prepared.

(3) Preparation of ethyl acetate extract from Jamdu

It was carried out in the same manner as in Example 1- (3), except that Jamdu was used, and as a result, ethyl acetate extract of Jamdu was prepared.

(4) Preparation of Jamdu hexane extract

It was carried out in the same manner as in Example 1- (4), except that Jamdu was used, and as a result, Jamdu hexane extract was prepared.

(5) Preparation of Jamdu hot water extract

It was carried out in the same manner as in Example 1- (5), except that Jamdu was used, and as a result, Jamdu hot water extract was prepared.

(6) Preparation of Jamdu ultra high pressure extract

Except for using jamdu, it was performed in the same manner as in Example 1- (6), and as a result, an ultrahigh pressure extract of jamdu was prepared.

(7) Preparation of subcritical extract of Jamdu

It was performed in the same manner as in Example 1- (7), except that Jamdu was used, and as a result, a subcritical extract of Jamdu was prepared.

Example 3. Preparation of swallow bean extract

(1) Preparation of swallow bean methanol extract

It was carried out in the same manner as in Example 1- (1), except that swallow beans were used, and as a result, swallow bean methanol extracts were prepared.

(2) Preparation of ethanol extract of swallow bean

It was carried out in the same manner as in Example 1- (2), except that swallow beans were used, and as a result, swallow bean ethanol extracts were prepared.

(3) Preparation of swallow bean ethyl acetate extract

It was carried out in the same manner as in Example 1- (3), except that swallow bean was used, and as a result, ethyl bean ethyl acetate extract was prepared.

(4) Preparation of swallow bean hexane extract

It was performed in the same manner as in Example 1- (4), except that swallow beans were used, and as a result, swallow bean hexane extracts were prepared.

(5) Preparation of swallow bean hot water extract

It was carried out in the same manner as in Example 1- (5), except that swallow beans were used, and as a result, swallow bean hot water extracts were prepared.

(6) Preparation of ultra-high pressure extract of swallow bean

It was carried out in the same manner as in Example 1- (6), except that the swallow bean was used, and as a result, a swallow bean ultra high pressure extract was prepared.

(7) Preparation of subcritical extract of swallow bean

It was performed in the same manner as in Example 1- (7), except that the swallow bean was used, and as a result, a swallow bean subcritical extract was prepared.

Experimental Example

(1) Analysis of muscle production activity

Preparation example

The muscle cell L6 myoblast was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and contains Dulbecco's modified Eagle's Media (DMEM; Hyclone) containing 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA). ) And cultured in a 6-well plate at 1.0 × 10 ⁵ cells / well. After incubation for 24 hours, the medium was exchanged with DMEM (Hyclone) containing 2% horse serum (HS; Hyclone) to differentiate for 6 days.

1-1) Muscle Generation Activity of Ethanol Extract from Eastern, Jamdu or Swallow Soybean

The mTOR activity of the ethanol extracts prepared in Examples 1 to 4 was evaluated using mTOR Sandwich ELISA kit (Cell Signaling Technology, Beverly, MA, USA).

The ethanol extracts prepared in Examples 1- (2), 2- (2), and 3- (2) were dissolved in DMEM (Hyclone) at a concentration of 40 ug / ml, and then treated with cells for 12 hours. After 12 hours, cells were lysed by treating the cell lysis buffer. Proteins were quantified at 1 mg / ml using the Bradford (Bio-Rad Laboratories Inc., Hercules, CA, USA) method, and then incubated at 37 ° C. for 2 hours at 50 µL by dispensing 50 μL into microwells attached with mTOR antibodies. After washing a total of 4 times with a washing buffer, the detection antibody was incubated for 1 hour at 37 ° C. After washing a total of 4 times with a washing buffer, HRP-linked secondary antibody was added and incubated at 37 ° C for 30 minutes. Finally, after washing a total of 4 times with washing buffer, the TMB substrate was placed in each well, incubated for 10 minutes at 37 ° C, and a stop solution was added to stop the reaction of TBM. After 2 minutes, absorbance was measured at a wavelength of 450 nm to measure mTOR activity. The results are shown in FIG. 1.

As a result, as shown in FIG. 1, it was confirmed that the activity of mTOR in L6 muscle cells was significantly increased ( ^* p <0.05, ^** p <0.01) by treatment of ethanol extract of eastern, jamdu or swallowtail. This means that the ethanol extract of eastern, persimmon or swallow bean of the present invention has excellent ability to increase muscle production in muscle cells.

1-2) Muscle Generation Activity of Eastern, Jamdu or Swallow Soybean Hot Water Extract

Except that the hot water extract prepared in Examples 1- (5), 2- (5), 3- (5) was used, it was performed in the same manner as in Experimental Example 1-1), and the results are shown in FIG. 2. Shown.

As a result, as shown in FIG. 2, it was confirmed that the activity of mTOR in L6 muscle cells was significantly increased ( ^* p <0.05, ^** p <0.01) by treatment of eastern, jamdu or swallow bean hot water extract. This means that the eastern, persimmon or swallow bean hot water extract of the present invention has an excellent ability to increase muscle production in muscle cells.

1-3) Muscle generation activity of subcritical extract of eastern, jamdu or swallow bean

It was carried out in the same manner as in Experimental Example 1-1) except that 5 ug / ml of the subcritical extract prepared in Examples 1- (7), 2- (7), and 3- (7) was used. The results are shown in FIG. 3.

As a result, as shown in FIG. 3, it was confirmed that the activity of mTOR was significantly ( ^** p <0.01) increased in L6 muscle cells by treatment with subcritical extracts of Jamdu or Swallow bean. This means that the sub-critical extract of Jamdu or Swallow bean of the present invention has excellent ability to increase muscle production in muscle cells.

(2) Analysis of activity to improve athletic performance

Preparation example

After incubating COS7 monkey kidney cells (ATCC) at 1.5 × 10 ⁵ cells / well in a 24-well plate, using lipofector (Aptabio, Yongin, Korea), pGL3-PGC-1α-Luc plasmid (Addgene, Cambridge, MA) , USA). After 4 hours transfection, cells were stabilized for 24 hours.

2-1) Dongbu, Jamdu, or Swallow Soybean Ethanol Extract improves exercise performance

PGC-1α activity was performed by luciferase assay to evaluate the effects of Dongbu, Jamdu or Vietbean ethanol extract on exercise performance.

The ethanol extracts prepared in Examples 1- (2), 2- (2), and 3- (2) were dissolved in DMEM (Hyclone) at a concentration of 40 ug / ml, and then treated with cells for 24 hours. After the final 24 hours, cells were washed with a phosphate buffer solution (PBS), and then lysed with NP-40 lysis buffer (Elpis, Daejeon, Korea), and luciferase assay substrate (Promega, Madison, WI, USA) was added. Then, the luciferase activity of PGC-1α was measured using a MicroLumat Plus LB 96V luminometer (Berthold, Wildbad, Germany), and the results are shown in FIG. 4.

As a result, as shown in FIG. 4, it was found that the ethanol extract of eastern, jamdu, or swallow bean significantly increased the activity of PGC-1α, a major factor involved in exercise performance, ( ^** p <0.01). Therefore, it was confirmed that the ethanol extract of Dongbu, Jamdu, or Swallow Soybean enhances athletic performance.

2-2) Dongbu, Jamdu or Swallow Soybean hot water extract's ability to improve exercise performance

Except that the hot water extract prepared in Examples 1- (5), 2- (5), 3- (5) was used, it was performed in the same manner as in Experimental Example 2-1), and the results are shown in FIG. 5. Shown.

As a result, as shown in FIG. 5, it was found that the extract of Jamdu hot water significantly increased the activity of PGC-1α, a major factor involved in exercise performance, ( ^** p <0.01). Therefore, it was confirmed that Jamdu hot water extract enhances exercise performance.

2-3) Dongbu, Jamdu or Swallow Soybean subcritical extract activity enhancing activity

It was carried out in the same manner as in Experimental Example 1-1) except that 5 ug / ml of the subcritical extract prepared in Examples 1- (7), 2- (7), and 3- (7) was used. The results are shown in FIG. 6.

As a result, as shown in FIG. 6, it was found that the subcritical extract of swallow bean significantly increases the activity of PGC-1α, a major factor involved in exercise performance, ( ^** p <0.01). Therefore, it was confirmed that the swallow bean subcritical extract enhances exercise performance.

Hereinafter, a formulation example of a composition containing the extract of the present invention will be described, but the present invention is not intended to limit it, but only to be specifically described.

Formulation Example 1: Preparation of powder

20 mg of eastern, pea or violet bean extract of the present invention

Lactose hydrate 100 mg

Talc 10 mg

The ingredients were mixed and filled in an airtight fabric to prepare a powder.

Formulation Example 2: Preparation of tablets

10 mg of eastern, pea or violet bean extract of the present invention

Corn starch 100 mg

Lactose hydrate 100 mg

Magnesium stearate 2mg

After mixing the above components, tablets were prepared by tableting according to a conventional tablet manufacturing method.

Formulation Example 3: Preparation of capsule

10 mg of eastern, pea or violet bean extract of the present invention

Microcrystalline cellulose 3 mg

Lactose hydrate 14.8 mg

Magnesium stearate 0.2 mg

After mixing the above components, a capsule was prepared by filling the gelatin capsule with a method for preparing a conventional capsule.

Formulation Example 4: Preparation of injection

10 mg of eastern, pea or violet bean extract of the present invention

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Hydrogen monohydrogen phosphate 26 mg

After mixing the above components, it was prepared with the above-mentioned ingredient content per ampoule (2 mL) according to a conventional injection preparation method.

Formulation Example 5: Preparation of liquid formulation

10 mg of eastern, pea or violet bean extract of the present invention

Isomerized sugar 10 mg

Mannitol 5 mg

Purified water

Lemon flavor

Dissolve each of the above components by adding each component to purified water according to a conventional manufacturing method, add an appropriate amount of lemon scent, add purified water, adjust to 100 mL, and sterilize to fill the brown bottle to prepare a liquid.

Formulation Example 6: Preparation of health functional food

10 mg of eastern, pea or violet bean extract of the present invention

Vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

Vitamin B ₁₂ 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ folic acid

Calcium Pantothenate 0.5 mg

Suitable amount of mineral mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium phosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 30 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for preparing a healthy food. , Granules can be prepared and used in the preparation of healthy food compositions according to conventional methods.

Formulation Example 7: Preparation of health drinks

Eastern, pea or swallow bean extract of the present invention 10 mg

Vitamin C 15 g

Vitamin E (powder) 100 g

Iron lactate 19.75 g

Zinc oxide 3.5 g

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B ₁ 0.25 g

Vitamin B ₂ 0.3 g

Purified water quantitation

After mixing the above components according to a conventional health drink manufacturing method, after stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered, obtained in a sterilized 2 liter container, sealed and sterilized, then stored in the refrigerator and then stored in the present invention. It is used for the preparation of healthy beverage compositions.

Although the above composition ratio is a mixture of components suitable for a preference drink in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

The above description of the present invention is for illustration only, and those skilled in the art to which the present invention pertains can understand that the present invention can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

As described above, since the Eastern, persimmon or swallow bean extract according to the present invention shows an effect of increasing muscle production, improving muscle endurance or athletic ability, the composition comprising the Eastern, persimmon or swallow bean extract of the present invention prevents muscle disease, Not only can it be usefully used for improvement or treatment, it can also be useful as a pharmaceutical composition for improving athletic performance, a food composition for improving athletic performance, or a food composition for improving muscle function.

Claims (12)

Pharmaceutical composition for the prevention or treatment of muscle diseases comprising the eastern extract as an active ingredient.
According to claim 1,
The extract is water, an organic solvent having 1 to 6 carbon atoms, or a mixture thereof, as a solvent, characterized in that the extraction of muscle disease prevention or treatment pharmaceutical composition.
According to claim 1,
The extract is a pharmaceutical composition for preventing or treating muscle disease, characterized in that is performed by subcritical fluid or supercritical fluid extraction.
According to claim 1,
The eastern extract is a pharmaceutical composition for the prevention or treatment of muscle disease characterized in that the extraction is performed at ultra high pressure.
According to claim 1,
The muscle disease is a pharmaceutical composition for preventing or treating muscle disease, characterized in that the muscle disease due to muscle function degradation, muscle wasting or muscle degeneration.
The method of claim 5,
The muscle disease is one or more diseases selected from the group consisting of atony, muscle atrophy, muscle dystrophy, myasthenia, cachexia and sarcopenia. Pharmaceutical composition for preventing or treating diseases.
A health functional food composition for preventing or improving muscle disease comprising the eastern extract as an active ingredient.
Food composition for improving muscle function, including the eastern extract as an active ingredient.
A pharmaceutical composition for improving exercise performance, including the eastern extract as an active ingredient.
The method of claim 9,
The improvement in exercise performance is characterized by preventing or treating one or more diseases selected from the group consisting of degenerative diseases, mitochondrial disorders, endurance, hypokinesia, lethargy, muscle destruction and depression, etc., for improving exercise performance Pharmaceutical composition.
A food composition for improving exercise performance, containing eastern extract as an active ingredient.
The method of claim 11,
The composition is a food composition for improving exercise performance, characterized in that it has an effect of increasing exercise execution time (exercise endurance), strengthening muscle strength, enhancing balance, and / or enhancing exercise adaptation.

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