KR20210158444A - Composition for improvement, treatment or prevention of muscular disorders, or improvement of muscular functions comprising Java tumeric or xanthorrhizol - Google Patents

Abstract

The present invention relates to a new use of xanthorrhizol or Java turmeric such as Java turmeric grind or extract and relates to a composition for muscular disorder improvement, treatment, or prevention or muscular function improvement containing Java turmeric or xanthorrhizol as an active ingredient. With the composition of the present invention, the activity of mTOR involved in muscle protein synthesis can be increased and the mRNA expression of MuRF-1 and atrogin-1 involved in muscle protein breakdown can be decreased. As a result, an increase in muscle mass and muscle function improvement can be achieved to be utilized in preventing, improving, or treating various muscular disorders such as atony, muscular atrophy, muscular dystrophy, muscle degeneration, myasthenia, cachexia, and sarcopenia.

Description

Composition for improvement, treatment or prevention of muscular disorders, or improvement of muscular functions comprising Java tumeric or xanthorrhizol

The present invention relates to a composition for improving, treating or preventing muscle disease, or improving muscle function, and more particularly, Java tumeric or xanthorrhizol such as Java turmeric powder or Java turmeric extract. ) relates to a composition for improving, treating or preventing muscle disease, or for improving muscle function, containing as an active ingredient.

Muscle atrophy is caused by a gradual decrease in muscle mass, and refers to muscle weakness and degeneration (Cell, 119(7):907-910, 2004). Muscular atrophy is promoted by inactivity, oxidative stress, and chronic inflammation, and weakens muscle function and athletic performance (Clinical Nutrition, 26(5):524-534, 2007). The most important factor that determines muscle function is muscle mass, which is maintained by a balance between protein synthesis and breakdown. Muscular dystrophy occurs when protein degradation occurs more than synthesis (The International Journal of Biochemistry and Cell Biology, 37(10):1985-1996, 2005).

Muscle size is regulated by intracellular signaling pathways that induce anabolism or catabolism within the muscle. When a signal transduction reaction that induces synthesis rather than degradation of muscle protein occurs, muscle protein synthesis is increased, which appears as an increase in muscle size (hypertrophy) or an increase in the number of muscle fibers (hyperplasia) according to an increase in muscle protein (The Korea Journal of Sports) Science, 20(3):1551-1561, 2011).

Factors involved in muscle protein synthesis induce protein synthesis by phosphorylating downstream proteins starting from stimulation of phosphatidylinositol-3 kinase (PI3K)/Akt pathway in muscle cells. The activity of the mammalian target of rapamycin (mTOR) by PI3K/Akt signaling is recognized as a central growth signaling factor that integrates various growth signals in cells. mTOR contributes to muscle mass increase by inducing muscle protein synthesis by activating two factors that initiate mRNA translation, 4E-binding protein (4EBP1) and phosphorylated 70-kDa ribosomal S6 kinase (p70S6K) (The Korea Journal of Sports Science, 20(3):1551-1561, 2011; The International Journal of Biochemistry and Cell Biology, 43(9):1267-1276, 2011). On the other hand, when the forward box (FoxO), a transcription factor, moves from the cytoplasm to the nucleus, it increases the expression of atrogin-1 and MuRF1, the E3 ubiquitin ligase factors involved in protein degradation (Disease Models and Mechanisms, 6:25- 39, 2013). When their expression level increases, protein breakdown in the muscle is accelerated, resulting in a decrease in muscle mass. Therefore, promotion of mTOR activity and suppression of atrogin-1 and MuRF1 expression increases the amount of muscle protein and increases muscle mass.

Differentiation of muscle cells and muscle formation are regulated by various muscle regulatory factors. Among them, the induction of myogenin expression by the activity of MyoD is the most important factor in the fusion of myoblasts, and is involved in the formation of myotubes. The muscle fibers formed through this process are bundled to finally form a muscle (Cellular and Molecular Life Sciences, 70:4117-4130, 2013).

Java tumeric is a medicinal plant that mainly grows in tropical areas such as Indonesia, Malaysia and Thailand. Javaturmeric belongs to the Zingiberaceae family taxonomically, and the scientific name is Curcuma xanthorrhiza Roxb. Antibacterial effect (J. Microbiol., 46:228-232, 2008; Asian Pac. J. Trop. Biomed., 2:S637-S640, 2012), antioxidant effect (Evid. Based Complement) in relation to javaturmeric so far Alternat. Med. 2012:438356, 2012), anti-inflammatory effect (J. Functional Foods 30:282-289, 2017), anti-periodontal effect (J. Microbiol. Biotechnol., 28(8):1270-1281, 2018), etc. activity has been reported.

In addition, xanthorrhizol, a representative component contained in Java Turmeric, is antibacterial (Planta Med., 66:196-197; J. Antimicrobial Chemther., 57:1231-1234, 2006), anti-inflammatory (J. Environ. Patho. Toxicol. Oncol., 21:141-148, 2002), antihyperglycemia (Evid. Based Complement. Alternat. Med., 2014:205915, 2014), anti-aging (Phytother. Res., 23:1299- 1302, 2009) and the like have been reported to have activity.

However, prior to the present invention, Javaturmeric or Xanthorizol for the prevention and treatment of muscle disease, or improvement of muscle function was not known.

Accordingly, the present inventors have searched for natural substances that have excellent muscle function control activity and can be safely applied. As a result, Java tumeric or xanthorrhizol such as Java turmeric powder or Java turmeric extract is effective for muscle diseases. The present invention was completed by confirming that there is activity for improvement, treatment or prevention, or improvement of muscle function.

Accordingly, an object of the present invention is to provide a food composition for improving or preventing muscle disease, or for improving muscle function, containing Java turmeric, such as Java turmeric powder or Java turmeric extract, or xanthorisol of the following formula (1) as an active ingredient will provide

[Formula 1]

Another object of the present invention is to provide a pharmaceutical composition for treating or preventing muscle disease containing Java turmeric, such as a Java turmeric powder or a Java turmeric extract, or xanthorisol represented by the following formula (1) as an active ingredient.

[Formula 1]

Another object of the present invention is to provide a cosmetic composition for improving or preventing muscle disease, or for improving muscle function, containing Java turmeric, such as Java turmeric powder or Java turmeric extract, or xanthorisol represented by the following formula (1) as an active ingredient. will provide

[Formula 1]

Another object of the present invention is to improve or prevent muscle disease containing Java turmeric, such as Java turmeric powder or Java turmeric extract, as an active ingredient, or xanthorisol of Formula 1 below, as an active ingredient, or muscle function It is to provide a feed additive for improvement.

[Formula 1]

In order to achieve the above object, the present invention improves or prevents muscle disease containing Java tumeric such as Java turmeric powder or Java turmeric extract or xanthorrhizol of Formula 1 as an active ingredient. For, or provides a food composition for improving muscle function.

[Formula 1]

In addition, the present invention provides a pharmaceutical composition for treating or preventing a muscle disease containing Java turmeric, such as a Java turmeric powder or a Java turmeric extract, or xanthorisol represented by the following Chemical Formula 1 as an active ingredient.

[Formula 1]

In addition, the present invention provides a cosmetic composition for improving or preventing muscle disease, or for improving muscle function, containing Java turmeric, such as a Java turmeric powder or a Java turmeric extract, or xanthorisol of Formula 1 below as an active ingredient.

[Formula 1]

The present invention also provides a feed additive for improving or preventing muscle disease, or for improving muscle function, containing Java turmeric, such as a Java turmeric powder or a Java turmeric extract, as an active ingredient, or xanthorisol represented by Formula 1 below.

[Formula 1]

Javaturmeric or xanthorizole according to the present invention has an excellent effect in increasing the activity of mTOR involved in muscle protein synthesis and inhibiting the mRNA expression of MuRF1 and atrogin-1 involved in muscle protein degradation. In addition, by increasing muscle mass and improving muscle function, it is possible to prevent, treat or improve muscle function deterioration, muscle loss, etc. caused by various diseases. Since the present invention is a natural product, it can be safely used without side effects and can be used as a pharmaceutical, food, cosmetic or feed additive.

1 is a result confirming the effect of increasing the activity of mTOR in L6 muscle cells, according to the treatment with Java turmeric supercritical extract.

Hereinafter, the configuration of the present invention will be described in detail.

The present invention provides a food composition for improving or preventing muscle disease of Java tumeric or xanthorrhizol, such as a Java turmeric pulverized product or a Java turmeric extract, or for improving muscle function; A pharmaceutical composition for treating or preventing muscle disease containing Java turmeric or xanthorizole, such as Java turmeric powder or Java turmeric extract; Or Java turmeric powder or Java turmeric extract, such as Java turmeric or xanthorisol for improving or preventing muscle disease, or cosmetic composition for improving muscle function; Or Java turmeric powder or Java turmeric extract for improving or preventing muscle disease of Java turmeric or xanthorisol, or feed additive for improving muscle function; Or it provides a method for treating a muscle disease comprising applying Java turmeric or xanthorizole, such as a Java turmeric powder or a Java turmeric extract, to a human or non-human mammal.

As used herein, 'Java tumeric' belongs to the ginger family (Zingiberaceae), and is a Java turmeric pulverized product or Java tumeric extract.

In the present specification, 'Java turmeric pulverized product' refers to a method that can be easily performed by a person skilled in the art to which the present invention pertains to the dried root (rhizome) of Java turmeric, mammals including humans While this can be easily ingested, the active ingredient is easily released from the dry pulverized material in the intestine after ingestion, and as a result, it can be prepared and used in a form that can be easily absorbed into the body of a mammal. For this purpose, the shape or size of the dry pulverized particles is not limited, but as the surface of the particles is maximized, it is easier to release the active ingredient described above, so it is preferable to prepare it in the form of a fine powder as much as possible.

In the present specification, 'Java turmeric extract' is obtained by extracting dried Java turmeric roots using a suitable solvent, and an extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a prepared product thereof or It includes all forms of purified products. The method for preparing the Java turmeric extract may be obtained by extracting from Java turmeric with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, and ultra-high pressure, subcritical or supercritical fluid, but is not limited thereto. .

The Java turmeric extract of the present invention can be prepared using conventional extraction methods in the art, such as heating extraction, cold-chill extraction, ultrasonic extraction, filtration, and reflux extraction, and Java turmeric is commercially available, or Naturally harvested or grown ones can be used.

The Java turmeric extract according to the present invention can be separated using a conventional solvent according to a conventional method known in the art for preparing an extract from a natural product, that is, under conditions of conventional temperature and pressure.

As used herein, the term 'fraction' refers to a result obtained by a fractionation method for separating a specific component or a specific group from a mixture containing various components. In the present invention, it refers to the result obtained by the fractionation method for isolating a specific component or a specific group from the Java turmeric extract prepared as described above.

In order to obtain the javaturmeric fraction according to the present invention, a conventional fractionation solvent known in the art, for example, a polar solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms such as water, ethanol, and methanol, and hexane, butanol , a non-polar solvent such as ethyl acetate, chloroform, dichloromethane, or a mixed solvent thereof may be used, but is not limited thereto.

The Java turmeric fraction of the present invention may include those obtained by additionally applying a purification process. For example, a fraction obtained by passing the Java turmeric extract according to the present invention through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) The fractions obtained through various purification methods additionally carried out such as separation by ) are also included in the Java turmeric extract of the present invention.

In the present specification, xanthorisol includes a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof.

[Formula 1]

The Xanthorisol of the present invention is a representative physiologically active ingredient present in Java Turmeric, and can be obtained by separating and purifying Java Turmeric, or it can be synthesized or purchased.

In this specification, 'muscle' refers to tendons, muscles, and tendons comprehensively, and 'muscular function' means the ability to exert force by contraction of the muscle, It includes muscle strength, which is the ability to perform, muscular endurance, which is the ability to repeat contractions and relaxations for how long or how many times a muscle can repeat contractions and relaxations with a given weight, and instantaneous power, which is the ability to exert strong force in a short period of time. As used herein, the term 'improving muscle function' refers to improving muscle function by increasing muscle mass.

The present invention relates to a food composition for improving and preventing muscle disease, or for improving muscle function, containing Java turmeric or xanthorisol as an active ingredient, such as a Java turmeric pulverized product or a Java turmeric extract; pharmaceutical compositions for preventing and treating muscle diseases; A cosmetic composition for improving and preventing muscle disease, or improving muscle function; It provides a feed additive for improving or preventing muscle disease, or for improving muscle function.

In one embodiment, the Java turmeric extract may be a hot water extract, an ethanol extract, an ethyl acetate extract, a hexane extract, an ultra-high pressure extract, a subcritical extract, or a supercritical extract, but is not limited thereto.

In one embodiment, the Java turmeric extract can be obtained by extracting the root of Java turmeric with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, subcritical and supercritical fluids. It can also be obtained by extracting Java turmeric under ultra-high pressure conditions of 100 MPa or more. If necessary, it may be prepared by additionally including filtration and concentration steps according to methods known in the art.

In one embodiment, the organic solvent having 1 to 6 carbon atoms is alcohol, acetone, ether, benzene, chloroform, ethyl acetate, It may be at least one selected from the group consisting of methylene chloride, hexane, cyclohexane, and petroleum ether.

In a specific embodiment of the present invention, the present inventors repeatedly extracted the dried pulverized product of Java turmeric root at room temperature using ethanol, ethyl acetate and hexane as solvents, hot water extraction, ultra-high pressure extraction, subcritical fluid extraction, A javaturmeric extract was prepared using supercritical fluid extraction.

As a result, the activity exhibited in the muscle cells was confirmed by treating the dried Java turmeric roots with hot water, 50% ethanol, 100% ethanol, ethyl acetate, hexane, ultra-high pressure, subcritical, supercritical extract, and xanthorisol to L6 muscle cells, respectively. , it was confirmed that the protein expression of p-mTOR involved in muscle protein synthesis was significantly increased (Fig. 1, Table 1). In addition, as a result of confirming the activity displayed in the muscle cells by treatment with each of the Java turmeric extract and xanthorisol in L6 muscle cells, it was confirmed that the mRNA expression of MuRF-1 and atrogin-1, which are involved in muscle protein degradation, was inhibited ( Table 2).

Immobilization using a skin stapler Using a mouse animal model, Java turmeric powder, Java turmeric hot water extract, Java turmeric ethanol extract, and Java turmeric supercritical extract were treated. As a result, muscle strength and muscle weight were improved. Each significantly increased compared to the control group (Table 3, Table 4). In addition, it was confirmed that as a result of treatment with naanthorisol, muscle strength and muscle weight were significantly increased compared to the control group.

In the case of the food composition for preventing and improving muscle disease or improving muscle function of the present invention, it can be used for preventing or improving muscle disease due to muscle wasting or degeneration. Muscle wasting and degeneration occurs due to genetic factors, acquired factors, aging, etc., and muscle wasting is characterized by a gradual loss of muscle mass, weakness and degeneration of muscles, particularly skeletal or voluntary muscles and cardiac muscles. Examples of related diseases include atony, muscular atrophy, muscular dystrophy, muscle degeneration, myasthenia gravis, cachexia and sarcopenia. The composition of the present invention has an effect of increasing muscle mass, and the type of muscle is not limited.

The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food, food additives and feed, and includes animals including humans or livestock. target for eating. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. Common foods include, but are not limited to, beverages (including alcoholic beverages), fruits and their processed foods (eg, canned fruit, bottled, jam, marmalade, etc.), fish, meat and their processed foods (eg, ham, sausages) Corned beef, etc.), breads and noodles (eg udon noodles, soba noodles, ramen, spagate, macaroni, etc.), fruit juice, various drinks, cookies, syrup, dairy products (eg butter, cheese, etc.), edible vegetable oils and fats, margarine , vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.) can be prepared by adding a Java turmeric powder or a Java turmeric extract. In addition, the nutritional supplement is not limited thereto, but it can be prepared by adding a Java turmeric powder or a Java turmeric extract to capsules, tablets, pills, and the like. In addition, the health functional food is not limited thereto, but for example, liquefied, granulated, and It can be ingested by encapsulation and powder. In addition, in order to use the Java turmeric, the Java turmeric pulverized product or the Java turmeric extract in the form of a food additive, it may be prepared and used in the form of a powder or a concentrate. In addition, it can be prepared in the form of a composition by mixing the pulverized Java turmeric or the Java turmeric extract and a known active ingredient known to be effective in preventing and improving muscle disease, or improving muscle function.

When the composition for preventing and improving muscle disease or improving muscle function of the present invention is used as a health drink composition, the health drink composition may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional drink. . The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as taumatine, stevia extract; A synthetic sweetener such as saccharin or aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

The pulverized Java turmeric or Java turmeric extract may be contained as an active ingredient in a food composition for preventing and improving muscle disease or improving muscle function, the amount of which is used for preventing and improving muscle disease or improving muscle function. Although not particularly limited to an effective amount to achieve, it is preferably 0.01 to 100% by weight based on the total weight of the total composition. The food composition of the present invention can be prepared by mixing with other active ingredients known to be effective in preventing and improving muscle disease or improving muscle function together with the Java turmeric powder or Java turmeric extract.

In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid, pectic acid salts, alginic acid, alginic acid salts, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, It may contain glycerin, alcohol or a carbonation agent and the like. In addition, the health food of the present invention may contain fruit for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The composition for preventing and treating muscle disease of the present invention may also be a pharmaceutical composition. When the composition for preventing and treating muscle disease of the present invention is a pharmaceutical composition, it may be used for the prevention or treatment of muscle disease caused by muscle wasting or degeneration. Muscle wasting and degeneration occurs due to genetic factors, acquired factors, aging, etc., and muscle wasting is characterized by a gradual loss of muscle mass, weakness and degeneration of muscles, particularly skeletal or voluntary muscles and cardiac muscles. Examples of related diseases include atony, muscular atrophy, muscular dystrophy, muscle degeneration, myasthenia gravis, cachexia and sarcopenia. The composition of the present invention has an effect of increasing muscle mass, and the type of muscle is not limited.

The 'pharmaceutical composition for preventing and treating muscle disease of the present invention may include a pharmaceutically acceptable carrier.

The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. Carriers for parenteral administration may also include water, suitable oils, saline, aqueous glucose and glycols, and the like. In addition, it may further include a stabilizer and a preservative. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives are benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. As other pharmaceutically acceptable carriers, reference may be made to those described in the following literature (Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995).

The pharmaceutical composition of the present invention may be administered to mammals including humans by any method. For example, it may be administered orally or parenterally, and parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal. , intranasal, enteral, topical, sublingual or rectal administration.

The pharmaceutical composition of the present invention may be formulated as a formulation for oral administration or parenteral administration according to the administration route as described above. When formulated, one or more buffers (eg, saline or PBS), antioxidants, bacteriostatic agents, chelating agents (eg, EDTA or glutathione), fillers, bulking agents, binders, adjuvants (eg, aluminum hydroxide) side), suspending agents, thickening agents, wetting agents, disintegrating agents or surfactants, diluents or excipients.

Solid preparations for oral administration include tablets, pills, powders, granules, liquids, gels, syrups, slurries, suspensions or capsules, and such solid preparations include at least one excipient in the pharmaceutical composition of the present invention, for example , starch (including corn starch, wheat starch, rice starch, potato starch, etc.), calcium carbonate, sucrose, lactose, dextrose, sorbitol, mannitol, xylitol, erythritol maltitol, cellulose , methyl cellulose, sodium carboxymethyl cellulose, and hydroxypropylmethyl-cellulose or gelatin may be mixed and prepared. For example, tablets or sugar tablets can be obtained by blending the active ingredient with a solid excipient, grinding it, adding a suitable adjuvant, and processing it into a granule mixture.

In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, or syrups. In addition to water or liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, flavoring agents, or preservatives may be included. .

In addition, in some cases, cross-linked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may be added as a disintegrant, and an anti-aggregating agent, a lubricant, a wetting agent, a flavoring agent, an emulsifier, and a preservative may be additionally included. .

When administered parenterally, the pharmaceutical composition of the present invention may be formulated according to methods known in the art in the form of injections, transdermal administrations and nasal inhalants together with suitable parenteral carriers. In the case of the injection, it must be sterilized and protected from contamination by microorganisms such as bacteria and fungi. For injection, examples of suitable carriers include, but are not limited to, solvents or dispersion media containing water, ethanol, polyols (eg, glycerol, propylene glycol and liquid polyethylene glycol, etc.), mixtures thereof and/or vegetable oils. can More preferably, suitable carriers include Hanks' solution, Ringer's solution, PBS (phosphate buffered saline) with triethanolamine or isotonic solutions such as sterile water for injection, 10% ethanol, 40% propylene glycol and 5% dextrose. etc. can be used. In order to protect the injection from microbial contamination, it may further include various antibacterial and antifungal agents such as parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In addition, in most cases, the injection may further contain an isotonic agent such as sugar or sodium chloride.

In the case of transdermal administration, forms such as ointment, cream, lotion, gel, external solution, pasta, liniment, and air are included. In the above, 'transdermal administration' means that an effective amount of the active ingredient contained in the pharmaceutical composition is delivered into the skin by topically administering the pharmaceutical composition to the skin.

In the case of administration by inhalation, the compounds for use according to the invention may be administered in pressurized packs or using a suitable propellant, for example, dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. It can be conveniently delivered in the form of an aerosol spray from the nebulizer. In the case of a pressurized aerosol, the dosage unit may be determined by providing a valve to deliver a metered amount. For example, gelatin capsules and cartridges for use in inhalers or insufflators may be formulated to contain a powder mixture of the compound and a suitable powder base such as lactose or starch. Formulations for parenteral administration are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a recipe commonly known to all pharmaceutical chemistry.

When the pharmaceutical composition for preventing and treating muscle disease of the present invention contains the java turmeric extract or xanthorizol in an effective amount, it can provide a desirable preventive and therapeutic effect on muscle disease. As used herein, the term 'effective amount' refers to an amount that exhibits a higher response than that of the negative control group, and preferably refers to an amount sufficient to improve muscle function. The pharmaceutical composition of the present invention may contain 0.01 to 99.99% of java turmeric extract or xanthorisol, and the remaining amount may be occupied by a pharmaceutically acceptable carrier. The effective amount of the java turmeric extract or xanthorisol contained in the pharmaceutical composition of the present invention will vary depending on the form in which the composition is commercialized.

The total effective amount of the pharmaceutical composition of the present invention may be administered to a patient as a single dose, or may be administered by a fractionated treatment protocol in which multiple doses are administered for a long period of time. The pharmaceutical composition of the present invention may vary the content of the active ingredient depending on the severity of the disease. When administered parenterally, it is preferably administered in an amount of 0.01 to 50 mg, more preferably 0.1 to 30 mg per kg body weight per day based on the java turmeric extract or xanthorizol, and when administered orally, java turmeric Based on the extract or xanthorizol, it may be administered in 1 to several divided doses so as to be administered in an amount of preferably 0.01 to 100 mg, more preferably 0.01 to 10 mg per 1 kg of body weight per day. However, the dose of the java turmeric extract or xanthorizol is determined by considering various factors such as the age, weight, health status, sex, severity of disease, diet and excretion rate of the patient, as well as the administration route and number of treatments of the pharmaceutical composition. Since the effective dosage is determined, those of ordinary skill in the art in consideration of this can determine an appropriate effective dosage according to the specific use of the javaturmeric extract or xanthorizole for the prevention and treatment of muscle diseases. will be. The pharmaceutical composition according to the present invention is not particularly limited in its formulation, administration route and administration method as long as the effect of the present invention is exhibited.

The pharmaceutical composition for preventing and treating muscle disease of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, or biological response modifiers.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention may also be provided in the form of an external preparation containing a java turmeric extract or xanthorisol as an active ingredient.

When the pharmaceutical composition for the prevention and treatment of muscle diseases of the present invention is used as an external preparation for skin, additionally, a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, and a foaming agent (foaming agent) ), fragrance, surfactant, water, ionic emulsifier, nonionic emulsifier, filler, sequestering agent, chelating agent, preservative, vitamin, blocker, wetting agent, essential oil, dye, pigment, hydrophilic active agent, lipophilic active agent Or it may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used in external preparations for skin, such as lipid vesicles. In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

When the pharmaceutical composition for preventing and treating muscle disease of the present invention is provided as an external preparation for skin, it may be in the form of an ointment, patch, gel, cream or spray, but is not limited thereto.

The composition for preventing and improving muscle disease or for improving muscle function of the present invention may also be a cosmetic composition. The cosmetic composition of the present invention contains Java turmeric extract or Xanthorisol as an active ingredient, and a basic cosmetic composition (lotion, cream, essence, cleansing foam and cleansing water, such as face wash, pack, body oil) together with a dermatologically acceptable excipient. ), color cosmetic compositions (foundation, lipstick, mascara, makeup base), hair product compositions (shampoo, conditioner, hair conditioner, hair gel), and soap.

The excipient is not limited thereto, but may include, for example, an emollient, a skin penetration enhancer, a colorant, a fragrance, an emulsifier, a thickening agent, and a solvent. In addition, fragrances, dyes, bactericides, antioxidants, preservatives and moisturizing agents may be additionally included, and for the purpose of improving physical properties, thickeners, inorganic salts, synthetic polymer materials, and the like may be included. For example, in the case of manufacturing a face wash and soap with the cosmetic composition of the present invention, it can be easily prepared by adding Java turmeric extract or xanthorisol to a conventional face wash and soap base. In the case of preparing a cream, it can be prepared by adding Java turmeric extract or Xanthorisol to a general oil-in-water type (O/W) cream base. Here, synthetic or natural materials such as proteins, minerals and vitamins for the purpose of improving physical properties such as fragrances, chelating agents, pigments, antioxidants, and preservatives may be additionally added.

The content of the java turmeric extract or xanthorisol contained in the cosmetic composition of the present invention is not limited thereto, but is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight, based on the total weight of the composition. If the content is less than 0.001% by weight, the desired anti-aging or wrinkle improvement effect cannot be expected, and if it exceeds 10% by weight, there may be difficulties in safety or formulation.

The composition of the present invention may be added to a feed additive or a feed composition comprising the same for the purpose of preventing or improving muscle disease.

As used herein, the term 'feed additive' refers to a substance added to feed for various effects such as nutrient supplementation and weight loss prevention, enhancement of digestibility of fiber in feed, oil quality improvement, reproductive disorder prevention and fertility improvement, and prevention of high temperature stress in summer includes The feed additive of the present invention corresponds to an auxiliary feed under the Feed Management Act, and mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and complex minerals, and trace minerals such as zinc, copper, cobalt, selenium, and kerotene. , vitamins AD, E, nicotinic acid, vitamin B complex, etc., protective amino acids such as methionine and lysine, protective fatty acids such as fatty acid calcium salts, probiotics (lactic acid bacteria), yeast cultures, live bacteria such as mold fermented products, Yeast agents and the like may be further included.

In the present invention, the term 'feed' refers to any natural or artificial diet, one-meal meal, etc. or a component of the one-meal meal for animals to eat, ingest and digest, or to prevent or improve muscle disease according to the present invention. The feed containing the composition for use as an active ingredient can be prepared in various types of feed known in the art, and preferably, a concentrated feed, roughage and/or special feed may be included, but is not limited thereto.

In the enriched feed, seed fruits containing grains such as wheat, oats, and corn, bran containing rice bran, bran, barley bran, etc. It is a concentrated product obtained by concentrating the remaining starch residues, which are the main components of starch residues, which are by-products obtained from sesame cakes, sweet potatoes, potatoes, etc., fish meal, fish residues, and fresh liquids obtained from fish. Animal feed such as dried whey, yeast, fish soluble, meat meal, blood meal, cow meal, skim milk powder, milk from cheese, whey, which is the remainder of manufacturing casein from skim milk, yeast, Chlorella, seaweed, but not limited thereto.

For roughage, raw grass feed such as wild grasses, grasses and green cuttings, turnips for feed, beets for feed, root vegetables such as luther beargers, a type of turnip, raw herbs, green crops, grains, etc. are placed in a silo. Examples include, but are not limited to, silage, which is stored feed fermented with lactic acid after filling, wild grass, hay obtained by cutting and drying grass, straw of crops for breeding purposes, and leaves of legumes. Special feed includes mineral feed such as oyster shells and rock salt, urea feed such as urea or its derivative diureide isobutane, supplementing ingredients that are easily lacking when only natural feed raw materials are mixed, or added to formulated feed to increase feed storage. There are feed additives and dietary supplements, which are substances added in trace amounts, but are not limited thereto.

The feed additive for preventing or improving muscle disease according to the present invention can be prepared by adding a Java turmeric powder or a Java turmeric extract in an appropriate effective concentration range according to various feed preparation methods known in the art.

The feed additive according to the present invention can be applied without limitation as long as it is an individual for the purpose of preventing or improving muscle disease. For example, non-human animals such as cattle, horses, pigs, goats, sheep, dogs, cats, rabbits, etc., birds and fish can be applied to any object.

Hereinafter, the present invention will be described in more detail through examples.

However, the following examples are only illustrative of the present invention, and the content of the present invention is not limited to the following examples.

[Example 1] Preparation of Java Turmeric Extract

<1-1> Preparation of Java turmeric hot water extract

After the dried Java turmeric root was pulverized with a mixer, 10 g of the crushed Java turmeric root sample was added to 100 mL of water and extracted at 80° C. for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 1 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components, and then Java turmeric hot water extract was obtained.

<1-2> Preparation of Java Turmeric 50% Ethanol Extract

After grinding the dried Java turmeric root with a mixer, 10 g of the crushed Java turmeric root sample was placed in 100 mL of 50% ethanol and extracted at 40° C. for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 1 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components, and then a Java turmeric 50% ethanol extract was obtained.

<1-3> Preparation of Java Turmeric Ethanol Extract

After grinding the dried Java turmeric root with a mixer, 10 g of the crushed Java turmeric root sample was added to 100 mL of 100% ethanol and extracted at 40° C. for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 1 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components, and then a Java turmeric ethanol extract was obtained.

<1-4> Preparation of Java turmeric ethyl acetate extract

After the dried Java turmeric root was pulverized with a mixer, 10 g of the crushed Java turmeric root sample was added to 100 mL of ethyl acetate and extracted at 40° C. for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 1 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components, and then a Java turmeric ethyl acetate extract was obtained.

<1-5> Preparation of Java Turmeric Hexane Extract

After the dried Java turmeric root was pulverized with a mixer, 10 g of the crushed Java turmeric root sample was added to 100 mL of hexane and extracted at 40° C. for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 1 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components, and then a Java turmeric hexane extract was obtained.

<1-6> Preparation of ultra-high pressure Java turmeric extract

After grinding the dried Java turmeric root with a mixer, 1 g of the crushed Java turmeric root and 76 mL of 18% ethanol are placed in a polyethylene pack and sealed, followed by an ultra-high pressure extraction device (Frescal MFP-7000; Mitsubishi Heavy Industries) was extracted using Under the ultra-high pressure extraction conditions, the extraction pressure was 320 MPa and the extraction time was 5 min. The extracted sample was filtered with Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove solvent components to obtain a Java turmeric ultra-high pressure extract.

<1-7> Preparation of Java turmeric subcritical extract

After the dried Java turmeric root was pulverized with a mixer, 50 g of the crushed Java turmeric root was placed in a subcritical water reactor of a subcritical extraction device (Biovan, Gyeonggi, Korea) with 1 L water and sealed. After sealing, the temperature of the reactor was raised to 200°C, and when the temperature of the reactor reached 200°C, heating was stopped, and the temperature was maintained for 20 minutes for extraction. After 20 minutes, the extract was transferred to a storage tank supplied with cooling water and rapidly cooled to 30° C., and then centrifuged at 3,600 rpm for 30 minutes to separate floating residues, and only the supernatant was taken. By using a freeze dryer (Ilshin Lab Co. Ltd., Seoul, Korea) to remove all the solvent, a subcritical extract of Java turmeric root was obtained.

<1-8> Preparation of Java turmeric supercritical extract

The dried Java turmeric root was pulverized with a mixer, and 10 g of the crushed Java turmeric root was charged into a sample cartridge and extracted using a supercritical fluid extraction device (SFX 3560, Isco Inc., Lincoln, NE, USA). did The supercritical extraction conditions were an extraction pressure of 300 bar, an extraction temperature of 50° C., a flow rate of supercritical carbon dioxide of 60 mL/min, and an extraction time of 2 hours. When the supercritical fluid extraction was completed, the supercritical fluid state was released by lowering the pressure of the extraction device to obtain a Java turmeric root supercritical extract.

[Example 2] Effect of Java Turmeric Supercritical Extract to Promote Muscle Protein Synthesis

It is known that when mTOR protein is phosphorylated and activated, it can induce activation of proteins involved in muscle protein synthesis and increase in muscle mass in the PI3K/Akt signaling pathway in muscle cells. Accordingly, in order to confirm the muscle-generation inducing activity of Java Turmeric, the activity of mTOR was confirmed using the mTOR Sandwich ELISA kit (Cell Signaling Technology, Beverly, MA, USA).

L6 myoblasts (ATCC; Manassas, VA, USA) were mixed with Dulbecco's modified Eagle's media (DMEM; Hyclone) containing 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA) in a 6-well plate at 1 × It was cultured for 24 hours after seeding so as to become ^{10 5 cells/well.} After culturing, the medium in the well was removed, exchanged with DMEM (Hyclone) containing 2% horse serum (HS; Hyclone), and further cultured for 6 days to differentiate L6 cells into myotubes. Next, the Java turmeric supercritical extract of Example 1-8 was dissolved in DMEM (Hyclone) at concentrations of 0.5 μg/mL and 1.0 μg/mL, treated with cells and cultured for 12 hours. After incubation, cells were lysed by treatment with a cell lysis buffer. Proteins in the obtained cell lysate were quantified at a concentration of 1 mg/mL using Bradford (Bio-Rad Laboratories Inc., Hercules, CA, USA). 50 μL of cell lysate was dispensed into microwells to which anti-mTOR antibody was attached and left at 37° C. for 2 hours. After washing a total of 4 times with washing buffer, the detection antibody was treated and left at 37°C for 1 hour, washed again with washing buffer a total of 4 times, and then horseradish peroxidase ) was added to the conjugated secondary antibody and left at 37° C. for 30 minutes. Finally, after washing a total of 4 times with the washing buffer, the TMB substrate was put into each well and left at 37°C for 10 minutes, and the TMB reaction was stopped by adding a stop solution. After 2 minutes, absorbance was measured with a wavelength of 450 nm to measure the level of mTOR in myotube cells treated with Java turmeric root extract. The experiment was repeated three times in total, and the measured values obtained for each were calculated as a percentage (%) relative to the control and expressed as the mean ± standard deviation. Differences between groups were confirmed through Duncan multi-range analysis by one-way analysis of variance using the SPSS25.0 statistical package (SPSS Inc., Chicago, IL, USA). At this time, when the p value was less than 5%, it was marked as statistically significant.

As a result, as shown in FIG. 1 , it was confirmed that the activity of mTOR increased significantly ( ^** p < 0.01) compared to the control group by treatment with the Java turmeric supercritical extract. This means that the Java turmeric supercritical extract of the present invention has an excellent ability to promote muscle protein synthesis in muscle cells.

[Example 3] Effect of Java Turmeric Extract and Xanthorisol to Promote Muscle Protein Synthesis

mTOR activity was measured in the same manner as in Example 2, except that each of the Java turmeric extracts of Examples 1-1 to 1-7 was treated with 1.0 μg/mL each or xanthorizol was treated with 0.5 μg/mL. measured.

^{As a result, as shown in Table 1, it was confirmed that both the mTOR activity increased significantly (**} p < 0.01) as compared to the control group not treated with the extract according to the treatment with the java turmeric extract and xanthorisol. This means that the java turmeric extract and xanthorisol of the present invention have excellent ability to promote muscle protein synthesis in muscle cells.

experimental group Relative mTOR activity (%) Example 1-1 118.4±5.8** Example 1-2 120.6±8.2** Examples 1-3 123.7±8.4** Examples 1-4 124.9±7.5** Examples 1-5 128.1±11.6** Examples 1-6 125.5±7.3** Examples 1-7 129.2±9.6** nagging 142.3±10.2**

[Example 4] Inhibitory effect on muscle proteolysis of Java turmeric extract

L6 myoblast (ATCC), a muscle cell, was placed in a 6-well plate with DMEM (Hyclone) containing 10% FBS (Hyclone) at a concentration of 1 × 10 ⁵ cell/mL. When the cell density reached about 80-85%, the medium in the well was removed and the cells were treated with DMEM (Hyclone) containing 2% HS (Hyclone) to induce myotube differentiation. Differentiation was performed for a total of 6 days by replacing the medium with a new medium once every 2 days. After differentiation, the Java turmeric extract prepared in Examples 1-1 to 1-8 in DMEM (Hyclone) containing 50 ng/mL tumor necrosis factor alpha (TNF-α; PeproTech, Rocky Hills, NJ, USA) was dissolved in DMEM (Hyclone) at an amount of 1.0 μg/mL, respectively, and then treated with cells. After 12 hours, total RNA was isolated using TRIzol reagent (Takara, Otsu, Japan). The isolated total RNA was quantified using a nanodrop (NanoDrop 1000; Thermo Fisher Scientific Inc., Waltham, MA, USA). 16 μL of quantified RNA was analyzed using Reverse Transcriptase Premix (ELPIS-Biotech) and a PCR machine (Gene Amp PCR System 2700; Applied Biosystems, Foster City, CA, USA) at 42°C, 55 minutes and 70°C, for 15 minutes. It was synthesized as cDNA under conditions. Out of 16 µL of the generated cDNA, 1 µL of cDNA, the following specific primers (Bioneer, Daejeon, Korea) and PCR premix (ELPIS-Biotech) were used for 30 seconds at 95°C, 1 minute at 60°C, and 1 minute at 72°C. PCR was performed by repeating 30 times.

MuRF1

Forward primer: 5'-CCGGACGGAAATGCTATGGA-3' (SEQ ID NO: 1)

Reverse primer: 5'-AGCCTGGAAGATGTCGTTGG-3' (SEQ ID NO: 2)

Atrogin-1

Forward primer: 5'-GTTACTGCAACAAGGAGAATCTGTT-3' (SEQ ID NO: 3)

Reverse primer: 5'-CCGTATGAGTCTTATGTTTTGCTGG-3' (SEQ ID NO: 4)

β-Actin:

Forward primer: 5'-TGACAGGATGCAGAAGGAGATTAC-3' (SEQ ID NO: 5)

Reverse primer: 5'-TAAAACGCAGCTCAGTAACAGTC-3' (SEQ ID NO: 6)

As a result of PCR, the amplified cDNA was separated by electrophoresis on 1.5% agarose gel, and the cDNA band was confirmed using the G;BOX EF imaging system (Syngene).

The experiment was repeated three times in total, and the measured values obtained for each were calculated as a percentage (%) relative to the control and expressed as the mean ± standard deviation. Differences between groups were confirmed through Duncan multi-range analysis by one-way analysis of variance using the SPSS25.0 statistical package (SPSS Inc., Chicago, IL, USA). At this time, when the p value was less than 5%, it was marked as statistically significant.

As a result, as shown in Table 2, the mRNA expression levels of atrogin-1 and MuRF1 were significantly ^{increased ( ##} p < 0.01) compared to the control group by treatment with TNF-α, but when the Java turmeric extract was treated, TNF It was confirmed that the mRNA expression levels of MuRF1 and Atrogin-1 both decreased significantly ( ^** p < 0.01) compared to the -α-treated group. This means that the java turmeric extract of the present invention has excellent ability to inhibit muscle protein degradation in muscle cells.

experimental group Relative MuRF-1
Expression (%) Relative Atrogin-1
Expression (%) TNF-α 358.3±33.1 ^## 402.8±32.5 ^## Example 1-1 176.8±36.9** 235.4±28.7** Example 1-2 161.0±42.4** 226.9±35.5** Examples 1-3 150.7±36.8** 205.6±39.2** Examples 1-4 166.3±38.0** 230.4±30.8** Examples 1-5 146.1±49.7** 190.5±31.6** Examples 1-6 160.2±37.2** 205.0±35.1* Examples 1-7 174.8±33.2** 230.6±33.6** Examples 1-8 141.9±41.0** 180.4±38.2**

[Example 5] Xanthorizol muscle protein degradation inhibitory effect

Relative mRNA expression levels of MuRF1 and Atrogin-1 were measured under the same conditions as in Example 4, except that Xanthorisol was treated with 0.5 μg/mL.

As a result, when Xanthorizol was treated, the relative mRNA expression levels of MuRF1 and Atrogin-1 were 132.5±41.2% and 169.2±43.3%, respectively, which were significantly (** p < 0.01) decreased compared to the TNF-α treatment group. . This means that Xanthorizol of the present invention has an excellent ability to inhibit muscle protein degradation in muscle cells.

[Example 6] Muscle function and muscle mass increase effect of Java turmeric extract in animal model

<6-1> Breeding of animals and induction of muscle atrophy

As experimental animals, 7-week-old male mice (C57BL/6J; DBL, Korea) were purchased and the experiment was carried out. The breeding room environment of all animals was maintained at a temperature of 23 ± 2 °C and a relative humidity of 55 ± 10%. Before the start of the experiment, a total of 20 rats were randomly divided into 5 rats per group. After adaptation for 1 week, anesthesia was induced by intraperitoneal injection of 325 mg/kg of tribromoethanol (Sigma-Aldrich). After anesthesia, the right hindlimb gastrocnemius muscle and right sole of the rats in the muscle atrophy group and the sample administration group were cut with a stapler seam using a skin stapler (Unidus, Chungcheongbuk-do, Korea). The damage resulted in immobilization of the right hind leg, which was maintained for a week. After one week, the stapler shim fixed to the calf muscles and the soles of the feet was removed, and again for another week, 500 mg/kg of Javaturmeric dry pulverized product; 150 mg/kg of Javaturmeric hot water extract of Example 1-1; 150 mg/kg of Javaturmeric ethanol extract of Example 1-3; 150 mg/kg of the Java turmeric supercritical extract of Examples 1-8 was orally administered every day for 7 days. At this time, the normal group and the muscular atrophy group were orally administered with saline instead of the sample.

<6-2> Confirmation of muscle strength improvement effect of Java turmeric extract

After the oral administration period was over, the muscle strength of the rats was measured using a muscle strength meter (Panlab, Barcelona, Spain). The rat's tail was pulled with a constant force until the rat released the youngest member of the muscle strength meter, and a total of five consecutive tests were performed per rat.

As a result, as shown in Table 3, muscle strength was significantly ( ^# p < 0.05) decreased in the muscle atrophy group compared to the normal group, but the muscle strength was significantly ( ^** p < 0.01) was confirmed. This means that the pulverized Java turmeric and the Java turmeric extract of the present invention are excellent in the effect of increasing muscle strength reduced due to muscle atrophy.

experimental group strength (g) normal group 248.3±7.2 muscle atrophy 191.5±8.0 ^## Java Turmeric Powder 217.5±5.9** Java Turmeric Hot Water Extract 226.8±7.7** Javaturmeric Ethanol Extract 231.5±9.2** Javaturmeric Supercritical Extract 236.1±11.4**

<6-3> Confirmation of muscle weight increase effect of Java turmeric extract

After measuring muscle strength, 325 mg/kg of tribromomethanol (Sigma-Aldrich) was intraperitoneally injected into the experimental animals, and after anesthesia, the animals were sacrificed through deep blood sampling. After confirming that the heartbeat has stopped, the uninjured tibialis anterior muscle was removed from the right hind leg and the weight was measured.

As a result, as shown in Table 4, the weight of the tibialis anterior muscle of the muscular atrophy group was significantly ^{reduced ( ##} p < 0.01) compared to the normal group, but the weight was significant when treated with the Java turmeric powder and Java turmeric extract. ( ^** p < 0.01) was confirmed. This means that the pulverized Java turmeric and the Java turmeric extract of the present invention are excellent in the effect of increasing the muscle weight reduced due to muscle atrophy.

experimental group muscle weight (mg) normal group 57.9±2.1 muscle atrophy 46.0±2.4 ^## Java Turmeric Powder 52.1±3.6** Java Turmeric Hot Water Extract 54.5±2.9** Javaturmeric Ethanol Extract 55.1±3.1** Javaturmeric Supercritical Extract 56.7±3.0**

[Example 7] Effect of increasing muscle function and muscle mass of nagsol sol in an animal model

After inducing muscle atrophy in the same manner as in Example 6-1, xanthorisol was orally administered daily at a concentration of 20 mg/kg. Next, as a result of measuring muscle strength and muscle weight in the same manner as in Example 6-2 and Example 6-3, the naan-so-sol-administered group showed 27.5% of muscle strength and muscle weight compared to the muscle atrophy group, respectively ( ^** p < 0.01) and 19.2% ( ^** p < 0.01). This means that the naanthorisol of the present invention is excellent in the effect of increasing the muscle strength and muscle weight decreased due to muscle atrophy.

Hereinafter, a manufacturing example of a pharmaceutical, food or cosmetic containing the pulverized Java turmeric, the Java turmeric extract or xanthorisol as an active ingredient according to the present invention will be described, but the present invention is not intended to be limited thereto, but only a detailed description is to do The pharmaceuticals and foods of Preparation Examples 1 to 3 according to the following composition ingredients and composition ratios using the pulverized Java turmeric, Java turmeric extract or Xanthorisol, which are excellent in improving, treating or preventing muscle disease, or improving muscle function. Or the cosmetic composition was prepared according to a conventional method.

[Production Example 1] Pharmaceuticals

<1-1> Powder

After mixing 50 mg of the java turmeric extract or xanthorizol of the present invention and 2 g of crystalline cellulose, the powder was prepared by filling it in an airtight bag according to a conventional powder preparation method.

<1-2> Tablet

After mixing 50 mg of the java turmeric extract or xanthorizole of the present invention, 400 mg of crystalline cellulose, and 5 mg of magnesium stearate, tablets were prepared by tableting according to a conventional tablet manufacturing method.

<1-3> Capsules

After mixing 30 mg of the Java turmeric extract or xanthorizol of the present invention, 100 mg of whey protein, 400 mg of crystalline cellulose, and 6 mg of magnesium stearate, it was filled in a gelatin capsule according to a conventional capsule preparation method to prepare a capsule.

[Production Example 2] Food

<2-1> Manufacturing of health food

1000 mg of the pulverized Java turmeric or Java Turmeric extract of the present invention, 70 ug of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, 0.2 ug of vitamin B12, 10 of vitamin C mg, biotin 10 ug, nicotinic acid amide 1.7 mg, folic acid 50 ug, calcium pantothenate 0.5 mg, ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium monophosphate 15 mg, dibasic calcium phosphate 55 mg , 90 mg of potassium citrate, 100 mg of calcium carbonate, and 24.8 mg of magnesium chloride can be mixed and the mixing ratio may be arbitrarily modified. The granules can be prepared and used in the preparation of health food compositions according to a conventional method.

<2-2> Manufacturing of health drinks

1000 mg of Java turmeric extract of the present invention, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, and 1 g of taurine are added with purified water, and the above ingredients are mixed according to the general method for preparing a total 900 mL health drink, then about After stirring and heating at 85 for 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then stored in a refrigerator, and then used to prepare a health beverage composition.

<2-3> Chewing Gum

Chewing gum was prepared in a conventional manner by mixing 20% by weight of gum base, 76.9% by weight of sugar, 1% by weight of flavoring and 2% by weight of water and 0.1% by weight of the Java turmeric extract of the present invention.

<2-4> Candy

Sugar 60% by weight, starch syrup 39.8% by weight, and flavoring 0.1% by weight and 0.1% by weight of the Java turmeric extract of the present invention were mixed to prepare a candy in a conventional manner.

<2-5> Biscuit

1st grade strength 25.59%, 22.22% weight 1st grade, 4.80% by weight of refined sugar, 0.73% by weight of salt, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonium, 0.17% by weight of sodium bisulfite, 0.16% by weight of sodium bisulfite , rice flour 1.45% by weight, vitamin B 0.0001% by weight, milk flavor 0.04% by weight, water 20.6998% by weight, whole milk powder 1.16% by weight, milk substitute 0.29% by weight, monobasic calcium phosphate 0.03% by weight, dusting salt 0.29% by weight and spray A biscuit was prepared in a conventional manner by mixing 7.27 wt% of oil and 0.8301 wt% of the Java turmeric pulverized product or Java turmeric extract of the present invention.

[Production Example 3] Cosmetics

<3-1> Nutrient lotion (milk lotion)

Nutrient lotion was prepared according to a conventional method by using the Java turmeric extract of the present invention according to the formulation ratio of the nutrient lotion in Table 3 below.

Ingredients Preparation Example 3-1
(weight%) Javaturmeric Extract or Xanthorizole 2.0 squalane 5.0 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 liquid paraffin 0.5 Caprylic/Capric Triglycerides 5.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 Carboxyvinyl Polymer 0.1 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water to 100

<3-2> Softening lotion (skin lotion)

A softening lotion was prepared according to a conventional method by using the Java turmeric extract of the present invention according to the ratio of the softening lotion formulation in Table 4 below.

Ingredients Preparation 3-2
(weight%) Javaturmeric Extract or Xanthorizole 2.0 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water to 100

<3-3> Nourishing Cream

Nutrient cream was prepared according to a conventional method by using the Java turmeric extract of the present invention according to the formulation ratio of the nutrient cream in Table 5 below.

Ingredients Production Example 3-3
(weight%) Javaturmeric Extract or Xanthorizole 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hydrogenated castor oil 2.0 liquid paraffin 10 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 antiseptic appropriate amount pigment appropriate amount Spices appropriate amount Purified water to 100

<3-4> Massage cream

A massage cream was prepared according to a conventional method by using the Java turmeric powder or the Java turmeric extract of the present invention according to the massage cream formulation ratio shown in Table 6 below.

Ingredients Preparation 3-4
(weight%) Javaturmeric Extract or Xanthorizole 1.0 beeswax 10.0 Polysorbate 60 1.5 PEG 60 hydrogenated castor oil 2.0 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglycerides 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water to 100

<3-5> pack

A pack was prepared according to a conventional method by using the Java turmeric pulverized product or the Java turmeric extract of the present invention according to the ratio of the pack formulation in Table 7 below.

Ingredients Production Example 3-5
(weight%) Java Turmeric Powder or Java Turmeric Extract 1.0 polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservatives, Colors, Flavors appropriate amount Purified water to 100

<3-6> Gel

A gel was prepared according to a conventional method by using the Java turmeric extract of the present invention according to the gel formulation ratio shown in Table 8 below.

Ingredients Preparation 3-6
(weight%) Javaturmeric Extract or Xanthorizole 0.5 Sodium ethylenediamine acetate 0.05 glycerin 5.0 Carboxyvinyl Polymer 0.3 ethanol 5.0 PEG 60 hydrogenated castor oil 0.5 triethanolamine 0.3 Preservatives, Colors, Flavors appropriate amount Purified water to 100

As described above, the present invention is for improving, treating or preventing muscle disease containing Java tumeric or xanthorrhizol such as Java turmeric powder or Java turmeric extract, or for improving muscle function A composition is provided. More specifically, javaturmeric or xanthorizole of the present invention increases muscle mass and muscle strength by increasing the activity of mTOR and decreasing the mRNA expression levels of MuRF1 and atrogin-1, thereby improving, treating or preventing muscle disease, or muscle function. It shows an excellent effect on improvement. Accordingly, the present invention has high industrial applicability because it can provide a composition showing an excellent effect for improving, treating or preventing muscle disease, or for improving muscle function.

Claims (8)

A food composition for improving or preventing muscle disease, or for improving muscle function, comprising Java tumeric or xanthorrhizol represented by the following Chemical Formula 1 as an active ingredient.
[Formula 1]

The method of claim 1,
The Java Turmeric is a pulverized product of Java Turmeric roots,
The java turmeric extract is a food composition for improving or preventing muscle disease, characterized in that obtained by extracting the java turmeric root, or for improving muscle function. The method of claim 1,
The java turmeric extract is water, an organic solvent having 1 to 6 carbon atoms, subcritical fluid and supercritical fluid to improve or prevent muscle disease, characterized in that obtained by extracting java turmeric with one or more solvents selected from the group consisting of For, or a food composition for improving muscle function. 4. The method of claim 3,
The organic solvent is an alcohol having 1 to 6 carbon atoms, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane ( hexane), cyclohexane (cyclohexane) and petroleum ether (petroleum ether) for improving or preventing muscle disease, characterized in that at least one solvent selected from the group consisting of, or a food composition for improving muscle function. 5. The method according to any one of claims 1 to 4,
The muscle disease is characterized in that at least one selected from the group consisting of atony, muscular atrophy, muscular dystrophy, muscle degeneration, myasthenia gravis, cachexia and sarcopenia. A food composition for improving or preventing muscle disease, or for improving muscle function. A pharmaceutical composition for the treatment or prevention of muscle disease, comprising Javaturmeric or Xanthorisol represented by the following Chemical Formula 1 as an active ingredient.
[Formula 1]

A cosmetic composition for improving or preventing muscle disease, or for improving muscle function, comprising javaturmeric or xanthorisol represented by the following formula (1) as an active ingredient.
[Formula 1]

A feed additive for improving or preventing muscle disease, or for improving muscle function, comprising Java Turmeric or Xanthorisol represented by the following Chemical Formula 1 as an active ingredient.
[Formula 1]

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