KR20200048267A - The food composition for anti-obesity or anti-adult disease comprising pine nut cones by-products - Google Patents

Abstract

The present invention relates to an anti-obesity composition containing a Pinus koraiensis by-product extract. More specifically, the present invention relates to: an anti-obesity composition which uses a Pinus koraiensis by-product to increase utility value, and shortens the extraction time through steam distillation to within a few tens of minutes, thereby improving manufacturing efficiency while sufficiently extracting active components in Pinus koraiensis, and having an excellent anti-obesity effect; and functional food using the same.

Description

The composition of anti-obesity or anti-adult disease comprising pine nut cones by-products

The present invention relates to an anti-obesity or anti-inflammatory disease composition, and more particularly, to an anti-obesity or anti-inflammatory disease composition using pine tree by-products, in particular, a method for preparing a pine tree by-product extract and a pine tree by-product extract prepared accordingly. It relates to a composition for improving or preventing obesity or adult diseases.

Recently, the obesity population is increasing due to the development of industrial society and changes in the eating habits according to the improvement of living standards. The World Health Association estimates that in 2010, about 50% of Westerners are likely to become obese, and in Korea, the population of obesity is expected to increase significantly over time.

Obesity is a state in which body fat is excessively accumulated in the body, not a state of excess body weight, but a state in which fat is excessively accumulated in the body due to metabolic disorder. In other words, calorie intake exceeds the energy required for physical activity and growth, and excessive accumulation in fat tissue in the form of triglycerides causes obesity.

Obesity itself not only causes hair loss, discomfort, and inefficiency, but also causes diseases such as cardiovascular diseases such as hyperlipidemia, hypertension, arteriosclerosis, myocardial infarction, kidney failure, type 2 insulin-independent diabetes, and lung disease. And causes these so-called 5D phenomena that lead to death. Obesity is well known as a risk factor for cardiovascular diseases such as heart disease and stroke, liver disease, diabetes, cancer, and high blood pressure, as well as external problems. Obesity such as these currently affects all age groups, especially in older adults.

The mechanism that leads to obesity is due to the ingestion of excess sugars, which digests the sugars contained in food to become monosaccharides, is absorbed into the body through the small intestine, and the blood sugar rises and insulin secreted by the stimulus acts on fat cells to fatten the monosaccharides in the blood It is made to accept cells and turn them into fat. It is caused by complex causes such as other genetic factors.

In order to understand and treat diseases related to obesity, studies on fat metabolism and studies on diet, exercise therapy, behavior modification therapy, surgery therapy, and drug therapy have been actively conducted. Many studies are being conducted to have the effect of obesity.

Although anti-obesity foods or drugs are being developed for the treatment of obesity, they usually cause unpleasant sensations when taken, problems that need to be taken in excess, or are mostly chemical compounds, which can cause safety problems for the human body when administered regularly. have.

Treatment for obesity is classified into digestive control agents, appetite control agents, body fat synthesis inhibitors, and fat absorption inhibitors depending on the mechanism of action, but these chemicals treat obesity, while hypertension, vomiting, abdominal pain, gas bloating and fart, headache, There is a problem that side effects such as increased bowel movements may occur.

On the other hand, rapid post-prandial rise in blood and excessive insulin secretion due to excessive intake of carbohydrates (carbohydrates) have been reported to promote diabetes or hyperlipidemia in addition to obesity. It is thought that there is. Moreover, preventing hyperlipidemia is one of the effective methods of preventing atherosclerosis.

Therefore, a glycolytic digestive enzyme inhibitor, a monosaccharide absorption inhibitor, or an antihyperglycemic inhibitor has an effective effect as an antidiabetic agent, antihyperlipidemic agent, or anti-atherosclerotic agent.

Currently, carbohydrate digestive enzyme inhibitors used as pharmaceuticals include α-glucosidase inhibitor Acarbose (Bayer Pharmaceuticals Co., Ltd.) and postprandial hyperglycemic improver Bgribooz (A0-128: Takeda Pharmaceutical Co., Ltd.). In studies or clinical trials, a synergistic effect of post-prandial blood sugar levels was confirmed, and efficacy against anti-obesity and anti-diabetes was also reported. These have the action of excretion of cholic acid, lowering of cholesterol, lowering of triglycerides in blood, and inhibitory effects of lipase, and these are actions that inhibit the path leading to obesity by ingesting fat (triglycerides).

Since fat (triglyceride) is degraded by pancreatic lipase and absorbed through the small intestine, inhibiting lipase is believed to be useful as an anti-obesity by lowering triglyceride in the blood. In addition, it is useful as an antihyperlipidemic agent because it lowers serum lipids by inhibiting fat absorption into the intestinal tract.

Cholic acid (bile acid) adsorption and excretion agent inhibits exogenous cholesterol absorption by increasing the amount of excretion among feces by combining with cholic acid in the intestinal tract. That is, in order to compensate for the decrease in cholic acid by increasing the amount of excretion of cholic acid, catabolism from cholesterol to cholic acid rapidly progresses. It is believed that these actions lower blood cholesterol. Cholesterol is consumed by promoting the excretion of cholic acid, and promotes the breakdown of fat, which is a raw material of cholesterol.

As a medicament that has an excretion effect of cholic acid, there is cholstyramine, a hypercholesterolemia treatment agent, which is an anion exchange resin. By orally administering an anion exchange resin, the anion exchange resin adsorbs and fixes cholic acid in the intestinal circulation, hinders reabsorption of cholic acid, accelerates the conversion of cholesterol to cholic acid in the liver, and as a result, blood cholesterol concentration. It has the effect of lowering. However, cholestyramine, for example, has a dosage of 9 g suspended in 100 ml of water, so the dose is very high, and when taking it, the rough, unpleasant texture of the resin remains in the mouth, so the patient takes it very much There are difficult drawbacks.

As described above, a number of chemical synthetic compounds having respective actions have been reported, and some of them have been used as pharmaceuticals as described above, but there are many problems with both high doses and discomfort when taking them, and also because they are chemical synthetic compounds. When administered, the subject may feel anxious about the stability to the human body. In addition, although there is a great desire to prevent obesity in everyday life by mixing an anti-obesity agent with food, it has not been realized because it is a chemical synthetic compound or a large dose. There is always a need for the development of safe anti-obesity and / or anti-viral agents derived from natural products in response to such social demands.

As a natural substance derived from foods having anti-hyperglycemic activity and anti-obesity activity, chlorocitric acid and hydroxycitric acid, which are components of garcinia, have recently been known. However, nojirimycin, the active ingredient of epithelium, was too strong in activity to mix with food and drink. In addition, although it has been reported that the extract of guava leaves shows inhibitory activity against maltase and sucrase, it does not have sufficient blood glucose-suppressing activity or anti-obesity activity, and has not yet been developed as an anti-obesity agent. .

Therefore, recently, research for the development of pancreatic lipase inhibitors from foods and natural products without side effects has been conducted.

Looking at the results of the development of anti-obesity materials using natural substances, there are dietary fibers such as hydroxycitric acid (HCA), olivera, chicory, and inulin, which are involved in appetite suppression for each mechanism of action. Digestion and absorption of fat Chitosan, flovonoids, and the like are substances that inhibit. As substances that inhibit heat accumulation by inducing heat generation, there are cathepsin of red pepper, catechin of green tea, retinoic acid, and L-carnitine, conjugated linoleic acid (CLA), milk calcium and related proteins that regulate lipid metabolism. Is becoming. Herbal ingredients, fruit, onion, ginseng, green tea, etc. have been reported to be effective in weight control.

On the other hand, the pine tree is a tall tall tree belonging to the pine family and the scientific name is Pinus koraiensis Siebold & Zuccarine. Pine nuts are the best health foods, and pine nuts produced in Korea are fragrant and savory. It eats pine nut porridge when the body is weak, and is used for women's beauty, weakness, constipation, cough, and obesity.

In addition, American obesity expert Dennis Bruner has been found that pine nut extract of pine nuts is effective in treating obesity by lowering appetite. And pine nut extract inhibits fat production by regulating fat tissue related to fat differentiation, inhibits fat production process, inhibits fat accumulation, and has anti-obesity effect through suppression of fat cell differentiation.

Pine nut oil consists of unsaturated fatty acids such as palmitic acid, stearic acid, oleic acid, linoleic acid, eicosenoic acid and pinolenic acid. Oleic acid and linoleic acid.

On the other hand, edible oils using pine nuts that are currently commercially available in Korea are mostly produced by high temperature, high pressure, or solvent. Oil manufactured using high temperature and high pressure has a problem that most of its functional components are destroyed. In addition, the oil prepared by using a solvent has to further perform the solvent removal step, there is a problem that contains a lot of impurities.

As a technique for a composition using a pine tree by-product, it is disclosed in Korean Patent Registration No. 10-1787419 to use a pine tree pine by-product extract. However, this relates to a feed additive composition using pine tree pine by-product extract, and is not described as a composition for anti-obesity.

In addition, the Republic of Korea Patent No. 10-10-1413771 relates to a composition for inhibiting obesity containing pine needle extract, using a water vapor distillation extraction method, and is obtained by distillation at 80 to 150 ° C. for 3 to 4 hours. Here, pine nuts are used, and the distillation time is 3 to 4 hours, resulting in very low efficiency in terms of manufacturing due to long extraction.

Republic of Korea Registered Patent No. 10-1787419 Republic of Korea Registered Patent No. 10-1413771

The present invention for solving the above problems is to increase the usefulness of not only the pine nuts, but also the pine trees themselves, by using a by-product of the discarded pine tree.

In addition, the present invention can shorten the extraction time for pine tree by-products within 1 hour (within tens of minutes), and excellent anti-obesity and / or anti-inflammatory disease (anti-diabetic, anti-hyperlipidemic or anti-hyperlipidemic agents) It is for preparing an extract in which the effective ingredient having an effect of arteriosclerosis, etc.) is sufficiently extracted.

In addition, the present invention is to provide a food composition for anti-obesity or seongseongbyeong added to the effective component of the pine tree by-product extract.

To achieve the above object, a method for preparing a pine tree by-product extract according to an embodiment of the present invention comprises: crushing a pine tree by-product (S10); Extracting the pulverized pulverized product by steam distillation for a time in the range of 25 to 35 minutes with steam having a temperature in the range of 80 ° C to 90 ° C (S20); And cooling and condensing the extracted distillate (S30).

As an embodiment of the present invention, the pine tree by-products are at least one or more selected from branches obtained from pine trees, immature conifers, mature conifers, and roots, and extracting by the steam distillation method, the pulverized pulverized product is distilled into a distillation tank. Injecting, and supplying steam having a temperature in the range of 82 ° C to 88 ° C to the distillation tank for a time in the range of 27 to 33 minutes at a steam pressure in the range of 740 mmHg to 780 mmHg, wherein the cooling and condensation are After passing the extracted distillate through a cooler having a temperature in the range of 5 ° C to 10 ° C connected to the distillation tank, it is possible to obtain an oil or essential oil component through layer separation.

Another embodiment of the present invention is a food composition for improving or preventing obesity or adult diseases, comprising a pine tree by-product extract prepared by the above-described manufacturing method.

As described above, the present invention is characterized by using a by-product of the pine tree, and has an effect of increasing the utility value of the pine tree by-product discarded in addition to the pine tree.

In addition, the present invention shortens the extraction time by steam distillation method to within tens of minutes, thereby improving the production efficiency while sufficiently extracting the effective components in pine nuts, and having excellent anti-obesity and / or anti-infective diseases (anti-diabetic agents, anti-hyperlipidemic agents or anti- Anti-obesity or anti-inflammatory disease composition having an effect of arteriosclerosis).

In addition, the pine tree by-product extract according to the present invention has excellent alpha-glucosidase inhibitory activity, sucrose inhibitory activity, maltase inhibitory activity, and rapase inhibitory activity, so it can effectively suppress the accumulation of adipocytes. It may be used as a food composition and / or pharmaceutical composition for yongseong or seongseong.

1 is a flow chart showing a method of manufacturing a pine tree by-product extract prepared according to an embodiment of the present invention.
2 is a view showing the yield of the pine tree by-product extract (Phytocide Oil) according to the number of extraction times and extraction time of the pine tree by-products prepared according to Examples 1 to 5 of the present invention.
Figure 3 is a view showing the average value of the yield of pine tree by-product extract (Phytocide Oil) prepared according to Examples 1 to 5 of the present invention.
Figure 4 is a view confirming the effect of lipase inhibitory action according to the concentration of pine tree by-product extract prepared according to an embodiment of the present invention.

The present invention can be applied to a variety of transformations and may have various embodiments, and specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention. In the description of the present invention, when it is determined that detailed descriptions of related well-known technologies may obscure the subject matter of the present invention, detailed descriptions thereof will be omitted.

 The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this application, terms such as “include” or “have” are intended to indicate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, one or more other features. It should be understood that the existence or addition possibilities of fields or numbers, steps, operations, components, parts or combinations thereof are not excluded in advance.

Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from other components.

Throughout the specification of the present invention, when a step is said to be “on” or “before” another step, this is not only the case where one step is in direct time series relationship with the other step, but also with the mixing step after each step. Similarly, the two-step sequence can include the same rights as in an indirect time-series relationship where the time-series sequence can change.

The terms “about”, “substantially”, and the like used throughout the specification of the present invention are used in the sense or close to the value when manufacturing and material tolerances unique to the stated meaning are presented, and the present invention To aid in the understanding of the precise or absolute figures used to prevent unreasonable use by unscrupulous infringers of the disclosure, the terms “~ (steps)” or “steps” used throughout this specification are “ Does not mean "steps for."

Hereinafter, the present invention will be described in detail.

The inventors of the present invention have made great efforts to confirm the anti-obesity efficacy of the pine tree by-product extract, and to further elute functional ingredients that exert anti-obesity efficacy from the pine tree by-product extract, resulting in the present invention.

The pine tree by-product used in preparing the extract according to the present invention is called phytoncide oil, and is known to have an excellent anti-obesity effect, so it is desirable to minimize destruction of useful components and bioactive substances unique to pine trees during the extraction process.

To this end, the present invention is characterized by using a discarded pine tree by-product and shortening the extraction time to within tens of minutes.

1 is a flow chart showing a method of manufacturing a pine tree by-product extract prepared according to an embodiment of the present invention.

As shown in Figure 1, the present invention comprises a step (S10) of crushing (or washing and crushing) by-products of pine nuts, distillation of water vapor (S20), and cooling and condensation (S30).

First, the washing and crushing step (S10) is a process of washing and then crushing pine tree by-products.

The pine tree by-product used in the present invention may be used in the wild, and is not particularly limited thereto, and various known in the art may be used. For example, the pine tree by-products may use at least one selected from branches obtained from pine trees, immature, mature, and roots. Among them, it is preferable to use a by-product from which pine nuts of pine trees are shelled. Pine tree by-products are known to contain essential components such as terpene, phenolics, and tannin, as well as various components such as chlorophyll, inorganic, organic, and vitamin, and these components are related to obesity Anti-obesity and / or anti-inflammatory effects may be exhibited by exhibiting inhibitory activity on enzymes.

Washing the pine tree by-products may be selectively performed, and the washing method is not particularly limited, and various methods known in the art may be applied. For example, the pine tree by-products can be washed with running water 2-3 times as a whole. Washing the pine tree by-products is to obtain only pure pine tree extract by removing foreign substances before crushing the pine tree by-products.

The method of crushing the pine tree by-products is also not particularly limited, and includes various methods known in the art. For example, the washed pine tree by-products can be put in a grinder and crushed to a size of several centimeters. The crushing of the pine tree by-products is to ensure that the pine tree extract is effectively extracted in the extraction process by the steam distillation method described later.

Next, the steam distillation step (S20) is to extract useful components by steam distillation of the by-products washed and crushed in the step (S10).

In general, as a method of extracting a pine tree by-product, there are a solvent extraction method, a supercritical carbon dioxide extraction method, and a steam distillation method. Among them, the present invention uses a steam distillation method.

The water vapor distillation method is a method of extracting oil by generating water vapor at the bottom and separating it using a difference in boiling point between water and oil. Since it is extracted under atmospheric pressure, it prevents the extraction of unnecessary substances other than the pine tree by-product extract, and additional cost according to separation and purification The occurrence can be reduced. In addition, since the solvent is water vapor, there is an advantage that is harmless to the human body compared to the solvent extraction method. In addition, in the case of the supercritical carbon dioxide extraction method, there is a problem that the initial equipment cost is high, but on the other hand, the steam distillation method has the advantage of being able to extract at a relatively low cost.

Among them, the present invention is characterized in that the crushed pine tree Busan is extracted by steam distillation for a time in the range of 25 to 35 minutes with steam having a temperature in the range of 80 ° C to 90 ° C.

Specifically, according to the present invention, an exemplary method of extracting pine tree by-products by steam distillation may be as follows. First, by-products of pine nuts (preferably by-products after pine tree shelling are removed) are put into the extractor tank and steam is passed through the extractor tank for 25 to 35 minutes. At this time, the portion through which the steam passes is the bottom of the extractor tank, and the temperature of the steam in the interior of the extractor tank is preferably in a low temperature state of 80 ° C to 90 ° C. Then, the steam passes through the plant tissue streamline (oil bag) of the pine tree byproduct inside the extractor tank and is then discharged to the outside of the extractor tank.

In this process, if the temperature of the steam performed during distillation of the steam is less than 80 ° C, less effective component is extracted from the pine tree by-products, and when the temperature being performed exceeds 90 ° C, impurities other than the effective components of the pine tree by-products are not suitable. .

In addition, if the extraction time performed during the steam distillation is less than 25 minutes, the amount of extraction from the pine tree by-product is relatively small, and even if the extraction time exceeds 35 minutes, the increase in the extraction amount and yield of the extract is not large, so it is inefficient, and the extraction time is more In the case of being long, rather, the useful components were destroyed.

That is, as can be seen in the experimental examples described later, water vapor extraction showed a large difference in the extraction yield according to the extraction time. When extraction was performed for 60 minutes, the highest average yield, 1.377%, was obtained. However, when compared to the average yield of 30 minutes, 1.316%, there was no significant difference. In addition, considering that the yield when extracting for 20 minutes is 0.820%, and the difference between the yields after extracting for 20 minutes and 30 minutes is about 0.5%, the yield according to the time of extracting for 30 minutes and extracting for 60 minutes is It cannot be said to show a significant difference. That is, the present inventors first confirmed that the yield does not increase significantly with time even if extraction is performed for a time of 30 minutes or more. Therefore, it can be seen that proceeding with the extraction for 30 minutes shows the best results when comparing economic efficiency and energy and yield.

In addition, in the case of α-pinene, the main material of the pine tree extract, it was 55.83% when the extract was processed for 30 minutes and 57.84% when the extract was processed for 40 minutes. However, bicyclo [3.1.0] hex-2-ene, 4-Mehylene-1 (-1-Methylenthyl)-, cyclobutane, 1,2-bis (1-methylethenyl), starting from 30-minute and 40-minute extraction ) -Trans- and Caryophylene oxid are considered to be extinguished as the extraction process (or time) proceeds up to 40 minutes, so it seems to be sensitive to heat or volatilized under some influence on the extraction process. On the side, the extract of pine nuts, which was extracted for 30 minutes, showed three more components than the pine tree extract, which was processed for 40 minutes, which slightly increased the content of α-pinene, etc. It was confirmed that it disappeared. If you simply check the contents of α-pinene and β-pinene, which are representative materials of pine tree extract, the ingredients at 40-minute extraction may be considered to be more advantageous, but the physiological actions are not composed of one ingredient, but a combination of numerous ingredients As it occurs, the extract of pine nuts extracted by the water vapor extraction method is considered to be the optimal extraction time for about 30 minutes.

Further, it is preferable that the steam vapor pressure in the extractor tank embodying the water distillation method is about 760 mmHg, which is the atmospheric pressure level. When extracting essential oil components under normal pressure and / or high pressure conditions lower than atmospheric pressure (1 atm), benzaldehyde There is a disadvantage that the generation of harmful substances such as.

So, extracting by the steam distillation method, the pulverized pulverized material is introduced into a distillation tank, and steam having a temperature in the range of 82 ° C to 88 ° C is added to the distillation tank at a vapor pressure in the range of 740 mmHg to 780 mmHg for 27 minutes. It is preferred to feed for a time in the range of from 33 minutes.

Subsequently, the cooling and condensation step (S30) is to cool and condense the water vapor distillate extracted in the step (S20).

The method of cooling and condensing the water vapor distillate is not particularly limited, and various methods known in the art may be used.

Among them, the cooling and condensation, after passing the extracted distillate through a cooler having a temperature in the range of 5 ℃ to 10 ℃ connected to the distillation tank, it is possible to obtain an oil or essential oil component through layer separation. That is, the water vapor distillate obtained in step (S20) is preferably subjected to cooling at a temperature within the range of 5 ° C to 10 ° C, and more preferably, cooling at a temperature of 8 ° C. For example, a cooler is connected to the outside of the extractor tank, and the temperature when steam exits the extractor tank and passes through the cooler is preferably 5 ° C to 10 ° C. When the temperature of the steam is satisfied inside the extractor tank and the temperature of the steam outside, the extract is effectively extracted from the pine tree by-product.

As described above, the condensate obtained through the cooling and condensation step (S30) may be used as a pine tree by-product extract in the present invention. The pine nut extract obtained by the steam distillation method as described above may be obtained in the form of a clear, colorless and transparent oil (oil) having a pine nut flavor (distilled water extraction oil) through layer separation such as centrifugation.

In the present invention, in order to confirm the anti-obesity and / or anti-inflammatory effect of the oil or essential oil component thus obtained, the obesity-related enzymes α-glucosidase and sukra are prepared using pine tree by-product extract prepared according to the examples described below. Inhibitory activity was confirmed for aase, maltase, and lipase. As a result, the oil or essential oil component according to the present invention inhibits fat production by regulating adipose tissue related to fat breakdown, inhibits fat production process, inhibits fat accumulation, and suppresses fat cell differentiation to prevent anti-obesity and / Or has an stellar disease effect.

Another aspect of the present invention may be a pharmaceutical composition for the treatment or prevention of obesity or adult diseases, including the pine tree by-product extract prepared by the above-described manufacturing method. That is, according to the present invention, by effectively inhibiting lipid accumulation in adipocytes, it is possible to provide an anti-obesity or anti-obesity pharmaceutical composition comprising a pine tree by-product extract having excellent anti-obesity and / or anti-inflammatory effect.

In the present specification, adult disease is a major disease mainly occurring in adults and the elderly, and collectively refers to a disease mainly occurring after middle age. That is, it may include all diseases in which the metabolism is not smoothly performed in the body, the balance between consumption and new production is not achieved, or blood flow is not smooth due to lack of various hormones. For example, diabetes, hyperlipidemia, arteriosclerosis, and the like, may include hypertension, heart disease, kidney disease, angina pectoris, myocardial infarction, cerebral hemorrhage, encephalopathy, emphysema bronchiectasis, and various types of cancer diseases. Especially, it is preferable that it is diabetes, hyperlipidemia and / or arteriosclerosis which causes lipid accumulation in adipocytes.

In addition, the pine tree by-product extract prepared according to the present invention may be used as a health functional food for improving or preventing obesity or adult diseases.

The pine tree by-product extract contained as an active active ingredient in the dietary supplement of the present invention can be formulated with food, pharmaceutically acceptable carriers, excipients or diluents. The carrier, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose , Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, but are not limited thereto.

In addition, the dietary supplement of the present invention may include diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used, and other food and pharmaceutically acceptable additives.

As an embodiment of the present invention, the health supplements containing the pine tree by-product extract according to the present invention, respectively, according to a conventional method, powders, granules, tablets, capsules, emulsions, oral formulations such as aerosols, external preparations, suppositories or It may be formulated and used in the form of a sterile injection solution.

When formulated as a solid preparation for oral administration, tablets, pills, powders, granules, capsules, etc. are possible, and these solid preparations include at least one excipient in addition to the pine tree by-product extract, for example, starch, calcium carbonate ( It can be prepared by mixing calciumcarbonate), (sucrose or lactose, gelatin, etc ..) In addition to simple excipients, lubricants such as magnesium stearate can also be used.

When formulated as a liquid preparation for oral administration, suspensions, intravenous solutions, emulsions, syrups, etc. are possible, and in addition to the commonly used simple diluents, water and liquid paraffin, various excipients, such as wetting agents, sweeteners, fragrances, preservatives And the like.

When formulated as a formulation for parenteral administration, sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and suppositories are included. Non-aqueous solvents, suspending agents may be used, such as propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, but are not limited thereto. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerogelatin may be used, but is not limited thereto.

The preferred dosage of the food composition of the present invention varies depending on the patient's age, condition and weight, degree of obesity, drug form, administration route and duration, but can be appropriately selected by those skilled in the art. For the desired effect, the dosage of the composition of the present invention is 10 mg / kg to 5000 mg / kg per day for an adult male, preferably 20 mg / kg to 4000 mg / kg, per adult male, based on the extract content of pine tree by-product as an active ingredient.

The administration may be administered once a day, or may be divided into several times. However, the scope of the present invention is not limited by the dosage and frequency of administration.

The food composition of the present invention can be administered to various mammals, such as rats, mice, livestock, and humans, by various routes, for example, orally.

The present invention also provides the use of a pine tree by-product extract for the prevention or improvement of obesity or adult diseases, the use of a pine tree by-product extract for the production of food for the prevention or improvement of obesity or adult diseases, and a food-effective amount in mammals including humans. It provides a method for the prevention or improvement of obesity or adult diseases, including the administration of pine tree by-product extract. The present invention also provides a food composition for suppressing obesity or adult diseases containing pine tree by-product extract.

The food composition of the present invention can be used as a health functional food. "Healthy functional food" means food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act 627 of the Health Functional Food, and "functional" means the structure of the human body and It means to intake for the purpose of controlling nutrients for function or obtaining useful effects for health purposes such as physiological action.

The health functional food of the present invention may include ordinary food additives, and whether or not it is suitable as a "food additive" is a corresponding product according to the general rules and general test methods of food additives approved by the Food and Drug Administration, unless otherwise specified. It is judged according to standards and standards.

Items described in the "Food Additives Revolution" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamonic acid, natural additives such as chromosomes, licorice extract, crystalline cellulose, high-color pigments, and guar gum, L -Mixed preparations such as sodium glutamate, noodles-added alkalis, preservatives, and tar colorants.

The health functional food of the present invention can be variously used in foods and beverages for the prevention or improvement of obesity or adult diseases, such as various foods, beverages, gums, teas, vitamin complexes, health functional supplements, food additives, etc. Can be used in the form of a powder, granule, tablet, capsule or beverage. In addition to the above, it may be in any food form.

The health-sight food of the present invention may include, in the purpose of preventing or improving obesity or adult diseases, pineapple by-product extract in an amount of about 5 to 60% by weight relative to the total weight of the food. This range may vary depending on the individual's weight, age, gender, health status, diet, and severity of obesity.

For example, the health functional food in the form of tablets is granulated with a mixture of pine nuts by-product extract, excipients, binders, disintegrants, and other additives in a conventional manner, and then compressed with a lubricant or the like. It can be directly compressed. In addition, the health functional food in the form of tablets may contain a mating agent or the like as necessary, and may be coated with a suitable peeling agent if necessary.

 Among capsules of health functional food in the form of capsules, hard capsules may be prepared by filling a mixture of additives such as pine tree by-product extracts and excipients, or granular or peeled granules thereof in a conventional hard capsule, and soft capsules extracts pine tree by-products. , And a mixture with an additive such as an excipient may be prepared by filling a capsule base such as gelatin. The soft capsule agent may contain a plasticizer such as glycerin or sorbitol, a colorant, and a preservative, if necessary.

The health functional food in the form of a ring may be prepared by molding a mixture of pine tree by-product extract, excipient, binder, disintegrant, etc. in a suitable manner, and if necessary, prepare a sugar with white sugar or other suitable repellent, or starch, talc, or a suitable substance. You can also be greeted with.

The granular form of the health functional food can be prepared in a granular manner by mixing a mixture of pine tree by-product extract, excipient, binder, disintegrant, and the like, and may contain a flavoring agent and a mating agent as necessary.

The method for preparing the pine tree by-product extract described above will be described in more detail through specific examples.

<Example 1> Pine tree by-product extract 1 (extraction time 20 minutes)

The byproduct from which pine nuts were removed from the pine tree was washed and crushed. Then, 1,000 g of crushed by-products were put into an extractor distillation tank, and steam at 85 ° C. was passed through a pine tree by-product in the extractor tank for 20 minutes while maintaining a vapor pressure condition of 760 mmHg. And, the temperature when the steam passes through the cooler outside the extractor tank was set to 8 ° C, and the essential oil component obtained by separating the cooled condensate was prepared as a pine tree by-product extract according to the present invention.

16 samples were prepared to obtain the average extraction amount and the average yield, and the results shown in Table 1 and FIG. 2 shown below were obtained. In addition, the average extraction amount (g) was obtained from 16 samples and shown in FIG. 3.

Sample number Extraction frequency Total extraction amount (g) Average extraction amount per time (g) Average yield (%) One 7 57.67 8.23 0.823 2 9 66.67 7.40 0.74 3 9 66.67 7.40 0.74 4 11 80.00 7.27 0.727 5 5 42.00 8.40 0.84 6 10 72.33 7.23 0.723 7 10 91.33 9.13 0.913 8 11 80.00 7.27 0.727 9 4 30.00 7.50 0.750 10 10 75.67 7.57 0.757 11 10 83.67 8.37 0.837 12 4 34.33 8.57 0.857 13 10 97.33 9.73 0.973 14 7 51.33 7.33 0.733 15 10 98.33 9.83 0.983 16 5 49.67 9.93 0.993 Total number of extractions 132 averages 8.20 0.820

<Example 2> Pine tree by-product extract 2 (extraction time 30 minutes)

In Example 1, except that the passing time of the steam was 30 minutes, after preparing the pine tree by-product extract in the same manner as in Example 1, after obtaining the average extraction amount and average yield for this, Table 2 below 2 and 3.

Sample number Extraction frequency Total extraction amount (g) Average extraction amount per time (g) Average yield (%) One 3 53.33 17.77 1.777 2 4 42.67 10.67 1.067 3 8 112.00 14.00 1.400 4 2 20.00 10.00 1.000 5 9 90.00 10.00 1.000 6 4 53.33 13.33 1.333 7 4 54.00 13.50 1.350 8 5 66.33 13.27 1.327 9 6 75.00 12.50 1.250 10 2 31.67 15.83 1.583 11 10 119.00 11.90 1.190 12 8 115.00 14.37 1.437 13 11 148.33 13.47 1.347 14 10 125.67 12.57 1.257 15 10 142.00 14.20 1.420 16 5 65.85 13.17 1.317 Average number of extractions 101 times 13.16 1.316

<Example 3> Pine tree by-product extract 3 (extraction time 40 minutes)

In Example 1, except that the passing time of the steam was 40 minutes, after preparing the pine tree by-product extract in the same manner as in Example 1, after obtaining the average extraction amount and average yield for this, Table 3 below 2 and 3.

Sample number Extraction frequency Total extraction amount (g) Average extraction amount per time (g) Average yield (%) One 3 50.33 16.77 1.677 2 10 151.33 15.13 1.513 3 10 166.33 16.63 1.663 4 9 115.67 12.83 1.283 5 10 143.33 14.33 1.433 6 10 136.67 13.67 1.367 7 10 105.33 10.53 1.053 8 10 137.67 13.77 1.377 9 7 71.00 10.13 1.013 10 10 105.67 10.57 1.057 11 10 115.00 11.50 1.150 12 6 61.00 10.17 1.017 13 10 106.67 10.67 1.067 14 7 120.00 17.13 1.713 15 7 83.30 11.90 1.190 16 9 131.67 14.60 1.460 Average number of extractions 138 times 13.15 1.315

<Example 4> Pine tree by-product extract 4 (extraction time 50 minutes)

In Example 1, except that the passing time of the steam was 50 minutes, after preparing the pine tree by-product extract in the same manner as in Example 1, after obtaining the average extraction amount and average yield for this, Table 4 below And shown in FIGS. 2 and 3.

Sample number Extraction frequency Total extraction amount (g) Average extraction amount per time (g) Average yield (%) One 4 61.30 15.32 1.532 2 10 125.67 15.27 1.527 3 10 149.33 14.93 1.493 4 12 166.80 13.90 1.390 5 8 105.67 13.21 1.321 6 10 120.33 12.03 1.203 7 11 131.30 11.94 1.194 8 8 117.67 14.71 1.471 9 7 81.40 11.63 1.163 10 10 114.33 11.43 1.143 11 10 108.67 10.87 1.087 12 5 59.33 11.87 1.187 13 10 123.67 12.37 1.237 14 8 105.33 13.17 1.317 15 7 107.89 15.41 1.541 16 8 106.24 13.28 1.328 Average number of extractions 138 times 13.21 1.321

<Example 5> Pine tree by-product extract 5 (extraction time 60 minutes)

In Example 1, except that the passing time of the steam was 60 minutes, after preparing the pine tree by-product extract in the same manner as in Example 1, after obtaining the average extraction amount and average yield for this, Table 4 below And shown in FIGS. 2 and 3.

Sample number Extraction frequency Total extraction amount (g) Average extraction amount per time (g) Average yield (%) One 3 52.33 17.44 1.744 2 11 167.40 15.22 1.522 3 10 165.25 16.52 1.625 4 9 115.49 12.83 1.283 5 10 132.78 13.28 1.328 6 10 124.78 12.48 1.248 7 11 148.51 13.50 1.350 8 9 132.67 14.74 1.474 9 5 68.90 13.78 1.378 10 11 136.42 12.40 1.240 11 10 128.98 12.90 1.290 12 7 78.95 11.28 1.128 13 10 148.51 14.85 1.485 14 8 98.23 12.28 1.228 15 7 98.12 14.02 1.402 16 8 102.85 12.86 1.286 Total number of extractions 132 averages 13.77 1.377

<Comparative Example 1> Ultrasonic extract of half pomegranate leaves

For comparison with the above example, an ultrasonic extract of a pomegranate leaf was known to have α-glucosidase inhibitory activity related to obesity, sucrose inhibitory activity, and maltase inhibitory activity.

That is, the leaves obtained from the semi-precious stones were washed and crushed. Then, after the crushed semi-pomegranate leaf by-product 1,000 g was put into an extractor distillation tank, 2 L of distilled water was added, and extracted 10 times with an ultrasonic extractor at room temperature for 1 minute. Primary filtration using filter paper, refrigerated for 2 days, and when a precipitate formed, the final extract was obtained through secondary filtration.

< Experimental Example  1>: Comparison of extraction amount and yield according to extraction time

The water vapor extraction showed a big difference in the extraction yield according to the extraction time. When extraction was performed for 60 minutes, the highest average yield, 1.377%, was obtained. However, when compared to the average yield of 30 minutes, 1.316%, there was no significant difference. In addition, considering that the yield when extracting for 20 minutes is 0.820%, and the difference between the yields after extracting for 20 minutes and 30 minutes is about 0.5%, the yield according to the time of extracting for 30 minutes and extracting for 60 minutes is It cannot be said to show a significant difference. That is, even if extraction is performed for a time of 30 minutes or more, the yield does not increase significantly with time. Therefore, it can be seen that proceeding with the extraction for 30 minutes shows the best results when comparing economic efficiency and energy and yield.

Extraction time (min) Average extraction amount (g) yield(%) 20min 8.20 0.820 30min 13.16 1.316 40min 13.15 1.315 50min 13.21 1.321 60min 13.77 1.377

< Experimental Example  2> Analysis of useful ingredients and contents according to extraction time

Content analysis was conducted to find out the component content of the pine tree by-product extract prepared according to Examples 1 to 5 above. GC-MS equipment was used for the analysis, and the analysis conditions are as follows. The analysis results are shown in Table 7 below.

-Analyzer: AGILENT 6890/5973 GC / MSD

-Column: Innowax (60m x 0.32mm x 0.5um)

-Mobile phase gas: He

-Injection temperature: 260 ℃

-Oven temperature: 85 ℃ ~ 230 ℃ (2 ℃ / min)

-Desorption time: 1 min

Compounds (RT) 30min 40min α-pinene (9) 55.83 57.84 camphene (9.5) One 0.79 Bicyclo [3.1.0] hex-2-ene, 4-Mehylene-
1- (1-Methylethyl)-(9.802) 0.6 - β-pinene (10.9) 8.39 9.41 β-myrcene (12.068) 0.71 4.98 D-limonene (13.479) 1.82 21.22 cyclobutane,
1,2-bis (1-methylethenyl)-. Trans- (14.044) 1.02 - (+)-4-carene (17.561) 23.08 0.34 Bornyl acetate (29.591) 0.75 0.45 Tricyclo (5.4.0.0 (2,8) undec-9-ene,
2,6,6,9-tetramethyl (33.933) 0.6 0.15 α-Cubebene (35.648) 1.18 0.26 Seychellene (36.929) 2.51 0.27 Caryophyllene (37.889) 1.41 1.64 Caryophyllene oxide (46.423) 1.09 -

As shown in Table 7, the main material of the pine tree extract, α-pinene, showed 55.83% when the extract was processed for 30 minutes and 57.84% when the extract was processed for 40 minutes. In the case of β-pinene, which is the main substance of another pine tree extract, it increased slightly from 8.39% when the extract was processed for 30 minutes to 9.41% when the extract was processed for 40 minutes. And, starting from the 30-minute extraction and the 40-minute extraction, D-limonene had a large increase from 1.82% to 21.22%. Therefore, it can be seen that D-limonene increases the extraction time from 30 to 40 minutes.

However, "Bicyclo [3.1.0] hex-2-ene, 4-Mehylene-1 (-1-Methylenthyl)-", "cyclobutane, 1,2-bis (1-methylethenyl) -Trans-" and "Caryophylene oxid "Did not appear as the extraction time progressed to 40 minutes. In other words, it appears to be extinguished in the extraction process, and thus, it is considered to be volatile due to heat sensitivity or some influence on the extraction process. In other words, three types of components appeared in the pine tree extract that was extracted for 30 minutes, compared to the pine tree extract that was processed for 40 minutes, which slightly increased the content of α-pinene and β-pinene in extracts of 40 minutes or more It was confirmed that they disappeared.

According to this, if you simply check the contents of α-pinene and β-pinene, which are representative materials of pine tree extract, the components at 40-minute extraction may be considered to be more advantageous, but the physiological actions are not composed of one component, but many Since it is a combination of ingredients, when extracting pine tree by-products using a water vapor extraction method, about 30 minutes appears to be the most effective optimal extraction time.

Therefore, it was confirmed that Example 2 (extraction time 30 minutes) among the extracts of pine tree by-products prepared according to Examples 1 to 5 can obtain a sufficient amount of an extract, and that the most effective ingredients are not destroyed. . The experiment was conducted by applying the pine tree by-product extract according to Example 2 to the experimental example for confirming the inhibitory activity of various enzymes described below.

< Experimental Example  3> α-glucosidase inhibitory activity

For the obtained pine tree by-product extract, the inhibitory activity on α-glucosidase was measured by the following measuring method using yeast-derived α-glucosidase. Enzyme activity was measured by colorimetrically quantifying the increase in reducing power of glucose and fructose produced by hydrolysis of sucrose using dinitro salicylic acid. The results are shown in Table 8 below.

Method for measuring α-glucosidase inhibitory activity

-0.50 ml of 50 mM sucrose solution (50 mM potassium phosphate buffer at pH 7.0)

-α-glucosidase solution 0.25ml

-0.05ml of pine tree byproduct extract aqueous solution

-50mM potassium phosphate buffer, pH 0.77, 20ml

Specifically, the enzyme reaction solution obtained by mixing the sucrose solution with α-glucosidase, and the aqueous solution of the pine tree by-product extract prepared according to Example 2 is put in a test tube and reacted at 37 ° C. for 30 minutes. The resulting reduction stage was added with 1 ml of dinitrosalicylic acid solution (1% sodium hydroxide, 5% potassium tartrate, 0.2% phenol, 1% dinitrosalicylic acid, 0.05% sodium sulfite) and reacted at 100 ° C for 10 minutes, followed by 540 nm. Absorbance was measured at. As a control group, the potassium phosphate buffer solution was used instead of the sample solution indinitrosalicylic acid solution. In addition, as each blank, the potassium phosphate buffer was used instead of the enzyme solution (a mixture of sucrose solution, α-glucosidase solution, and pine tree by-product extract). The inhibitory activity is expressed by the inhibition rate obtained from the following equation. In addition, the ultrasonic extract (Comparative Example 1) of a half pomegranate leaf known as a substance having a plant-derived α-glucosidase inhibitory activity was also measured.

Inhibition rate (%) = {(A-B)-(C-D)} / (A-B) X 100

A: Absorbance of control solution

B: Absorbance of the control solution blank

C: absorbance of the sample solution

D: Absorbance of the sample solution blank

From the above results, the concentration of the aqueous solution of the pine tree by-product extract when the inhibition rate is 50% is determined as the IC ₅₀ value. The smaller the IC ₅₀ value, the stronger the enzyme inhibitory activity.

division Sample IC ₅₀ value (μg / ml) Example 2 Pine tree by-product extract 1.2 Comparative Example 1 Semi-pomegranate leaves (ultrasound extract) 19.0

Therefore, it can be seen that the pine tree by-product extract (Example 2) has higher α-glucosidase inhibitory activity than the semi-pomegranate leaf ultrasonic extract (Comparative Example 1), which has a relatively excellent anti-obesity effect.

< Experimental Example  4> Sucrase  Inhibitory activity

With respect to the pine tree by-product extract obtained above, using rat-derived rat small intestine acetone powder (manufactured by Sigma), it was measured by the following method for the activity of inhibiting sucrase against the small intestine-derived sucrase contained therein. Did. In addition, the enzyme activity was measured by lightening the increase in glucose produced by hydrolysis of sucrose with glucose c11-test Wako (manufactured by Wako Pure Chemical Industries Ltd.), a kit for measuring glucose. The results are shown in Table 9 below.

Method for measuring the inhibitory activity of sucrase

-0.50 ml of 50 mM sucrose solution (50 mM potassium phosphate buffer at pH 7.0)

-Rat small intestine acetone powder solution 0.25ml

-Pine tree by-product extract 0.05ml

-50mM potassium phosphate buffer, pH 7.00, 20ml

The enzyme reaction solution obtained by mixing the sucrose solution with α-glucosidase, and the aqueous solution of pine tree by-product extract prepared according to Example 2 is put in a test tube and reacted at 37 ° C. for 30 minutes. The produced glucose was measured by quantifying with glucose c11-test Wako (manufactured by Wako Junyaku), a kit for measuring glucose. As a control, the potassium phosphate buffer solution was used instead of the sample solution, dinitro salicylic acid solution. In addition, as each blank, the potassium phosphate buffer solution was used instead of the enzyme solution (a mixture of sucrose solution, α-glucosidase solution, and pine tree by-product extract). The inhibitory activity is expressed by the inhibition rate obtained from the following equation. In addition, a semi-pomegranate leaf, which is similarly known as a substance having a plant-derived sucrase inhibitory activity, was also measured for an ultrasonic extract (Comparative Example 1).

Inhibition rate (%) = {(A-B)-(C-D)} / (A-B) × 100

A: Absorbance of the control solution

B: Absorbance of the control solution blank

C: Absorbance of sample solution

D: Absorbance of the sample solution blank

From the above results, the concentration of the pine tree by-product extract when the inhibition rate is 50% is determined as the IC ₅₀ value. The smaller the IC ₅₀ value, the stronger the enzyme inhibitory activity.

division Sample IC ₅₀ value (μg / ml) Example 2 Pine tree by-product extract 900 Comparative Example 1 Semi-pomegranate leaves (ultrasound extract) 1000

Therefore, it can be seen that the pine tree by-product extract (Example 2) has higher sucrose inhibitory activity than the semi-pomegranate leaf ultrasonic extract (Comparative Example 1), which has a relatively excellent anti-obesity effect.

< Experimental Example  5> Maltase inhibitory activity

For the extract of pine tree by-products prepared according to Example 2, using rat-derived rat small intestine acetone powder (manufactured by Sigma), the inhibitory activity against maltase derived from small intestine contained therein was measured by the following method. In addition, the enzyme activity was measured by quantifying an increase in glucose produced by hydrolysis of maltose with glucose CII-Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.), a kit for measuring glucose. The results are shown in Table 10 below.

Method for measuring maltase inhibitory activity

-50mM maltose solution (using 50mM potassium phosphate buffer at pH 7.0) 0.50ml

-Rat intestine acetone powder solution 0.25 ml

-Pine tree by-product extract aqueous solution0.05ml

-50 mM potassium phosphate buffer, pH 7.00.20 ml

The enzyme reaction solution obtained by mixing the maltose solution with a rat small intestine acetone powder solution and a tamarind seed coat extract aqueous solution is put into a test tube (this is called solution A) and reacted at 37 ° C for 30 minutes. The resulting glucose was measured by quantifying with glucose CII-test Wako (manufactured by Wako Junyaku), a kit for measuring glucose. As a control, the potassium phosphate buffer was used instead of the A sample solution. In addition, as each blank, an enzyme solution (the potassium phosphate buffer solution was used instead of the pine tree by-product extract. The inhibitory activity is expressed by the inhibition rate obtained from the following equation. Also, similarly known as a substance having plant-derived sucrase inhibitory activity It was also measured for the ultrasonic extract of the pomegranate leaves (Comparative Group 1).

Inhibition rate (%) = {(A-B)-(C-D)} / (A-B) × 100

A: Absorbance of the control solution

B: Absorbance of the control solution blank

C: Absorbance of sample solution

D: Absorbance of the sample solution blank

From the above results, the concentration of pine tree by-product extract when the inhibition rate is 50% is determined as the IC ₅₀ value. The smaller the IC ₅₀ value, the stronger the enzyme inhibitory activity.

division Sample IC ₅₀ value (μg / ml) Example 2 Pine tree by-product extract 300 Comparative Example 1 Semi-pomegranate leaves (ultrasound extract) 400

Therefore, it can be seen that the pine tree by-product extract (Example 2) has a higher maltase inhibitory activity than the semi-pomegranate leaf ultrasonic extract (Comparative Example 1), which has a relatively excellent anti-obesity effect.

< Experimental Example  5> lipase inhibitory activity

With respect to the pine tree by-product extract prepared according to Example 2, a lipase inhibition test of tamarind dermal extract was performed using a pig pancreatic-derived lipase (manufactured by Sigma). The results are shown in Table 11 and Figure 4 below. Enzyme activity was measured by colorimetrically quantifying the increase in dimercaprol produced by hydrolysis of the lipase substrate dimercaprol tributyrate using 5'5-dithiobis-2-nitrobenzoic acid.

Measurement of lipase inhibitory activity

-5'5-dithiobis-2-nitrobenzoic acid solution (pH 7.5 buffer) 1.00 ml

-Lipase solution (pH 7.5 buffer) 0.05 ml

-0.10ml of pine tree byproduct extract aqueous solution

-Dimercaprol tributyrate solution 0.10ml

The enzyme reaction solution obtained by mixing the lipase solution with the pine tree by-product extract and dimercaprol tributyrate solution (sample A) is placed in a test tube and reacted at 30 ° C for 30 minutes. At the end of the reaction, 2.00 ml of an acidic anionic surfactant was added and the reaction of lipase was stopped, and absorbance was measured at 412 nm. As a control, distilled water was used instead of the liquid for sample A. In addition, as each blank, a buffer solution was used in place of the pineapple by-product extract of the enzyme solution. The inhibitory activity is expressed by the inhibition rate obtained from the following equation.

Inhibition rate (%) = {(A-B)-(C-D)} / (A-B) × 100

A: Absorbance of the control solution

B: Absorbance of the control solution blank

C: Absorbance of sample solution

D: Absorbance of the sample solution blank

From the above results, the concentration of pine tree by-product extract when the inhibition rate is 50% is determined as the IC ₅₀ value. And, the smaller the IC ₅₀ value, the stronger the enzyme inhibitory activity.

division Sample IC ₅₀ value (μg / ml) Example 2 Pine tree by-product extract 7.6

Therefore, it can be seen that the pine tree by-product extract (Example 2) has excellent lipase inhibitory activity, and thus has excellent anti-obesity effect.

In the above, the present invention has been illustrated and described in relation to a specific preferred embodiment, but the present invention may be variously modified and changed within the limits that do not depart from the technical features or fields of the present invention provided by the following claims. Being able is obvious to those of ordinary skill in the art.

Claims (3)

Crushing pine tree by-products (S10);
Extracting the pulverized pulverized product by steam distillation for a time in the range of 25 to 35 minutes with steam having a temperature in the range of 80 ° C to 90 ° C (S20); And
Cooling and condensing the extracted distillate (S30); containing, pine wood by-product extract manufacturing method.
According to claim 1,
The pine tree by-products are at least one selected from branches obtained from pine trees, immature conifers, mature conifers, and roots,
Extracting by the water vapor distillation method, the pulverized pulverized material is introduced into a distillation tank, and steam having a temperature in the range of 82 ° C to 88 ° C is added to the distillation tank at a vapor pressure in the range of 740 mmHg to 780 mmHg at 27 minutes to 33 minutes. To supply for an hour in the range of minutes,
The cooling and condensing, after passing the extracted distillate through a cooler having a temperature within a range of 5 ° C to 10 ° C connected to the distillation tank, obtains an oil or essential oil component through layer separation, Manufacturing method.
A food composition for improving or preventing obesity or adult diseases, comprising a pine tree by-product extract prepared by the method according to claim 1.

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