KR20160006812A - Compositions for preventing and curing diabetes comprising extract of Paeonia lactiflora - Google Patents

Abstract

The present invention relates to a pharmaceutical composition or a healthy food comprising an extract of Paeonia lactiflora PALL for preventing and treating diabetes and, more specifically, to a pharmaceutical composition comprising an extract of Paeonia lactiflora PALL, and a healthy food comprising an extract of Paeonia lactiflora PALL for preventing and treating diabetes confirmed to reduce blood sugar and increase secretion of insulin in an animal model of induced type 2 diabetes by controlling alpha-glucosidase.

Description

[0001] The present invention relates to a composition for prevention and treatment of diabetes comprising a peony extract,

The present invention relates to a composition for prevention and treatment of diabetes comprising Paeonia lactiflora extract, and more particularly to a composition for inhibiting alpha-glucosidase and a therapeutic effect for diabetes in vivo, A pharmaceutical composition useful for the prevention and treatment of type 2 diabetes, and a hypoglycemic-effective health functional food.

Due to the recent rapid improvement in living standards, the overconsumption of high calorie foods, lack of exercise, and the stress caused by the advancement of the industrial society are leading to the increasingly serious diseases. Representative adult diseases include hypertension, diabetes, obesity, and hyperlipemia. In particular, diabetes is the cause of all chronic vascular diseases.

Diabetes is the most common non-communicable disease occurring in modern people. When insulin produced in the pancreas is deficient or abnormal in function, diabetes-induced glucose is not properly stored in the liver, muscle, or adipose tissue and accumulated in the blood The fasting blood glucose level is maintained at 60 to 120 mg / dl, which is much higher than normal blood glucose levels of not more than 140 mg / dl for 2 hours after the meal. An excessive amount of blood glucose impairs the spermatogenesis, Or accumulated in the blood cholesterol or triglyceride. However, these symptoms are only acute symptoms that occur early in the onset of diabetes. Diabetes mellitus is a chronic disease that is delayed in treatment rather than the severity of the disease itself, such as complications such as blood circulation disorder, chronic fatigue, hypertension, myocardial infarction, diabetic neuropathy, retinopathy (visual disturbance, blindness, ), Cataract, nephrotic syndrome, etc., and the overall metabolic function and sensory function of the human body are deteriorated, which can cause fatal results in the past. Therefore, people with diabetic disease should slowly absorb the carbohydrates of the food they ingest and prevent the rapid increase of blood sugar. Alpha-Glucoshides, an enzyme involved in this, can be prepared by dissolving oligosaccharides, This enzyme inhibitor slows the postprandial increase in blood glucose by delaying the absorption of carbohydrates. Alpha-glucosidase inhibitors include acarbose and miglitol, which were developed by Bayer, Germany in 1977, and they are marketed as diabetes and obesity treatment drugs.

Peony is a perennial plant belonging to the family Paeoniaceae. It is native to parts of China, Siberia and Korea. The Chinese herbal medicine collection defines peel and herbal medicines as the root of Paeonia lactiflora Pallas or other herbaceous plants. These veterinary drugs and medicinal products contain monoterpene glycoside, acidic polysaccharide, tannin, etc., and have sedative action, platelet antiepileptive action, action on the circulatory system, antitumor action , Immunity enhancement, Anti-inflammatory action have been reported (Son Dong-Joo, Journal of the Korean Society of Food Science and Nutrition, 2002, 31, 511, Ibomi, Korea Pharmaceutical Association, 2008, 4, 445).

In the Oriental medicine, about 700 BC, the embarrassment of the Emperor of the Inner Circle, indicating the common consumable disease is one of the disease began to deal with diabetes. The treatment method, which administers acupuncture treatment combined with acupuncture treatment for 2 to 3 months, has been used as an old treatment method while intensively administering medicines such as chrysanthemum, rhubarb, and creation. Another folk remedy has been to use hemp, which is also called chrysanthemum and scab. Chunghwa has long been known as a covenant of diabetes because it is good at eliminating thirst due to diabetes, and it is known to protect the stomach of diabetic patients. Recently, there have been various studies on the treatment of diabetes using medicinal herbs.

Korean Patent Laid-Open Publication No. 2005-70704 discloses that the herbal extract used in Oriental medicine enhances the action of insulin in 3T3-L1 adipocytes, thereby increasing glucose uptake and remarkably suppressing alpha-glucoamylase activity. And a composition for preventing and treating diabetes mellitus containing the same as an active ingredient. Korean Patent Laid-Open Publication No. 2005-70704 discloses a composition for preventing and treating diabetes mellitus containing an extract of Yeast Extract which exhibits excellent activity to enhance the activity of AMPK (5'AMP-activated protein kinase) as an active ingredient. In addition to these, diabetes prevention and treatment methods using various herbal medicines or their extracts have been studied, and blood glucose lowering effect of the veterinary medicine has been reported. However, studies on the prevention and treatment of diabetes in peanut, which have superior anti- It is not.

Accordingly, the present inventors have repeatedly conducted studies on alpha-glucosidase inhibitory drugs. As a result, they have found that peony extracts strongly inhibit alpha-glucosidase and show excellent antidiabetic effects in diabetic animals. . Accordingly, an object of the present invention is to provide a pharmaceutical composition or health functional food for preventing and treating diabetes comprising Paeonia lactiflora extract, and a method for producing the same.

It is an object of the present invention to provide a pharmaceutical composition for preventing and treating diabetes comprising Paeonia lactiflora extract.

The pharmaceutical composition for preventing and treating diabetes mellitus has an excellent effect of inhibiting the absorption of glucose in the blood by inhibiting alpha-glucosidase and is particularly useful for the prevention and treatment of type 2 diabetes.

The peony extract may be in the form of a liquid extract, in the form of a dried powder, or in the form of a lyophilized powder.

It is an object of the present invention to provide a health functional food for hypoglycemia containing an extract of Paeonia lactiflora.

The above-mentioned health functional food for hypoglycemia has an excellent effect of inhibiting the absorption of glucose in the blood by inhibiting alpha-glucosidase, and is excellent in the blood glucose lowering effect and is useful as an antioxidant in diabetic patients, especially in patients with type 2 diabetes Is excellent.

The peony extract may be in the form of a liquid extract, in the form of a dried powder, or in the form of a lyophilized powder.

The health functional food for hypogonadism may be any one of tablet, capsule, powder, granule, liquid, and ring, and may be any one of a food, a powdered beverage, a solid, a chewing gum, a tea, a vitamin complex, And the like.

It is an object of the present invention to provide a pharmaceutical composition for the prevention and treatment of diabetes or a method for preparing a peony extract contained in a health functional food for hypoglycemic action.

The extract of Peony root can be obtained by extracting Paeonia lactiflora at 0 to 90 ° C with water, an alcohol having 1 to 5 carbon atoms or a mixed solvent thereof to prepare a peony root extract. have.

The peony extract of the present invention inhibits alpha-glucosidase and inhibits the absorption of glucose in the blood and inhibits the increase in blood glucose in vivo. Thus, it is possible to prevent and treat diabetes mellitus and its related diabetic complications, It can be used as a functional food. The peony extract of the present invention showed excellent efficacy in relation to blood glucose control, weight control, serum glucose and insulin content in a model of type 2 diabetes induction, and it was confirmed that the extract of the herb medicine had little side effects and excellent efficacy A composition for treating and preventing type 2 diabetes can be provided.

FIG. 1 is a graph showing changes in blood glucose levels in the different groups such as the group administered with the extract of peony root extract of the present invention and the group administered with the peony root extract.
FIG. 2 is a graph showing final fasting blood glucose levels in the group administered with the peony extract of the present invention and the group administered with the peony extract.
FIG. 3 is a graph showing insulin content in blood in the group administered with the peony extract of the present invention and the group administered with the peony extract, which is a comparative group.

Hereinafter, the present invention will be described in detail so that those skilled in the art can easily carry out the present invention.

The present invention relates to a peony root extract having an anti-diabetic effect, and provides a pharmaceutical or health functional food composition for the prevention and treatment of diabetes mellitus.

The peony root extract according to the present invention inhibits alpha-glucosides and inhibits the absorption of glucose in the blood, as well as the blood glucose lowering effect in the diabetic animal model, as compared with the black grape extract known as the existing blood glucose lowering effect.

The pharmaceutical composition for the treatment and prevention of diabetes having the above-mentioned composition according to the present invention comprises a peony root extract obtained by extracting with water, alcohol or a mixture thereof according to a conventional method to obtain an extract, And a peony extract in the form of a powdery dry extract.

The method for producing the above peony extract comprises the step of mixing the peony with an extraction solvent containing water, an alcohol or a mixture thereof, and extracting at 0 to 90 ° C, And then repeating extraction 2 to 5 times.

The content of alcohol in the extraction solvent is preferably in the range of 20 to 95% by weight, preferably 50 to 95% by weight, more preferably about 80% by weight, in terms of alcohol extraction efficiency. These alcohols may be used as long as they are commonly used in the art, preferably 1 to 5 carbon atoms, and the alcohol may be at least one selected from the group consisting of methanol, ethanol, isopropanol, propanol and butanol . More preferably, the alcohol may be ethanol, alcohol, or the like.

The peony root extract obtained by the above extraction process can be further roughened by fractionation and drying, and the peony root extract can be obtained in the form of a dry powdery powder by the lyophilization process during the drying process. When the lyophilization method is applied in the drying process, the loss of volatile organic substances in the extract can be reduced.

The thus obtained peony extract inhibited the absorption of glucose in the blood by inhibiting alpha-glucosidase, and confirmed the efficacy of reducing blood glucose in a diabetic animal model.

Therefore, the composition containing the peony extract can be effectively applied to the prevention and treatment of diabetes by inhibiting alpha-glucosidase.

When the composition of the present invention is used as a medicine, when the composition for the treatment and prevention of diabetes is used clinically, it may be formulated together with a carrier which is common in the pharmaceutical field to prepare a pharmaceutical agent such as tablets, capsules , Agents for oral administration such as troches, liquids, and suspensions; Injectable preparations in the form of injectable solutions or suspensions, or ready-to-use injectable dry powders which can be used as distilled water for injection for injection; Or ointments, and the like. The pharmaceutical preparations comminuted with conventional carriers may be administered orally or parenterally, for example intravenously, subcutaneously, intraperitoneally or topically.

The dosage of the composition for the treatment and prevention of diabetes according to the present invention varies from 0.01 mg / kg to 10 g / kg per day, preferably from 1 mg / kg to 1 g / kg per day ≪ / RTI > Generally, an adult dose of 10 to 500 mg, preferably 50 to 300 mg per day may be administered per day, and depending on the judgment of a doctor or pharmacist, several times a day, preferably once or six times May be administered in divided doses.

In addition, the health food refers to a food prepared by adding the above composition for treating and preventing diabetes to a general food, or encapsulated, powdered, or a suspension, and is intended to have a health-specific effect when ingested, , There is an advantage that there is no side effect that may occur when a food is used as a raw material for a long period of taking the medicine.

Specific examples thereof include various foods such as confectionery, processed foods, combination preservation, dairy products, and beverages. The shape of the food is not particularly limited, and may be a solid, semi-solid, gel, liquid, or powder.

The composition for the treatment and prevention of diabetes may be used in the form of general medicines, quasi-drugs, and health foods.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto.

Production Example 1: Preparation of peony extract

Paeonia lactiflora was obtained and pulverized. 1.8 kg of pulverized peonies were extracted with 10 ~ 20L of 80% ethanol repeatedly 2 ~ 5 times at room temperature to obtain 651 g (yield: 36.2%) of the extract.

Comparative Example 1

The extract was obtained in the same manner as in Example 1 except that Paeonia japonica was used.

Example 1: Measurement of alpha-glucosidase inhibitory activity

Alpha-glucosidase inhibitory activity of the peony root extract and peony root extract obtained in Preparation Example 1 and Comparative Example 1 was measured.

The enzyme alpha-glucosidase and para-nitrophenyl-glucopyranoside, a substrate, were purchased from Sigma, and 0.1 M phosphate buffer (pH 6.8) was directly prepared Respectively. After adding 10 쨉 l of the peony and albedo extracts to 1 쨉 l of 5 U alpha-glucosidase, 84 쨉 l of 0.1 M phosphate buffer and 5 쨉 l of 50 mM para-nitrophenyl-glucopyranoside as a substrate were added thereto, For 20 minutes.

After that, 200 μl of 2M NaOH was added to stop the reaction, absorbance was measured at 405 nm, and the concentration to inhibit 50% of alpha-glucosidase was determined based on the absorbance of DMSO instead of the extract , And the results thus measured are shown in Table 1.

IC ₅₀ ([mu] g / ml) 80% EtOH extract 38.6 ± 0.63 Peony 80% EtOH extract 25.0 + - 0.33

As shown in Table 1 showing the alpha-glucosidase inhibitory effect of the peony root extract, the peony root extract inhibited alpha-glucosidase at a lower concentration than the peony extract extract having a blood glucose lowering effect.

Example 2: Antidiabetic assessment in an animal model of type II diabetes

Long-term high-fat diets High-fat diets and low-density STZ-induced diabetic animals are widely used for type 2 diabetes studies because they cause insulin resistance but do not have diabetes (Chalkley et al., 2002) et al., 2006; Srinivasan et al., 2005).

Five-week-old ICR mice (males) were obtained from Raon Bio and purified for one week with free, regular solid feed and water. The environmental conditions of the animal room were set at 23 ± 3 ℃, 50 ± 10% of relative humidity, 12 hours of lighting time (8:00 am to 8:00 pm), 10 to 20 times of ventilation time, During the test period, the temperature and humidity of the animal room were automatically controlled by constant temperature humidification.

2) Streptozotocin (STZ) group, 3) high fat diet group, 4) control group of STZ + high fat diet group (diabetic group) and 5 groups of diabetic group ) Acarbose 20 mg / kg (positive control), 6) Buckwheat 50 mg / kg, 7) Buckwheat 100 mg / kg, 8) Peony 50 mg / kg and 9) Peony 100 mg / kg. Diabetes mellitus type 2 diabetes mellitus was induced by taking high fat diet for 3 weeks, which is the recovery period after induction of diabetes mellitus with low dose STZ. Blood glucose and body weight were measured at 1 week intervals with oral administration of each drug for 3 weeks. After the completion of the experiment, the blood was collected and serum was separated, and glucose and insulin contents in the serum were measured.

The results thus obtained are shown in Table 2 (change in body weight in an anti-diabetic animal test), and the graphs in Figs. 1, 2 and 3.

group Weight (g) Weight gain (g) Initial Final Normal diet 40.8 ± 2.56 42.7 ± 3.01 1.8 ± 1.33 STZ-treated group 40.2 ± 3.06 39.5 ± 2.59 -0.7 ± 2.42 High fat diet group 40.5 ± 0.84 43.0 ± 1.55 2.5 ± 0.84 STZ + high fat diet group 36.5 ± 4.72 35.2 ± 4.54 -1.3 ± 0.82 Acarbose 20 mg / kg group 37.8 ± 3.49 38.2 + 4.17 0.3 ± 1.51 Ginkgo 50 mg / kg group 35.3 ± 5.16 34.8 ± 4.45 -0.5 ± 1.22 Vaccine 100 mg / kg administered group 37.2 + 2.04 37.7 ± 2.34 0.5 ± 1.76 Peony 50 mg / kg administered group 40.7 ± 4.03 38.8 + 4.62 -1.8 ± 3.19 Peonies 100 mg / kg administered group 34.7 ± 4.08 36.3 ± 3.67 1.7 ± 4.03

As shown in Table 2, in the group administered with 100 mg / kg of peony and black lozenges extract, no weight loss due to diabetes was observed. In the group administered with 50 mg / kg, Respectively.

Meanwhile, as shown in FIG. 1, the blood glucose-lowering effect was somewhat weaker than that of the positive control group, but the blood glucose control effect was similar to that of the acarbose-treated group at 100 mg / kg of the peony and pine needle extract extracts. As a result of the measurement of blood glucose, it was confirmed that the peony extract-treated group was inhibited by about 10.3% more than the peony extract-treated group as shown in FIG.

As shown in FIG. 3, the insulin content in the blood was similar to that of the acarbose-treated group in the 100 mg / kg phytase extract group, and the insulin content of the peony extract-treated group was increased by about 5.3% Respectively.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (7)

A pharmaceutical composition for the prevention and treatment of diabetes, comprising a Paeonia lactiflora extract. The pharmaceutical composition for the prevention and treatment of diabetes according to claim 1, wherein the diabetes is type 2 diabetes. The pharmaceutical composition for the prevention and treatment of diabetes according to claim 1, wherein the composition for preventing and treating diabetes inhibits alpha-glucosidase and inhibits absorption of glucose in blood. The pharmaceutical composition for preventing and treating diabetes according to claim 1, wherein the peony extract is in the form of a liquid extract or a powder in which the extract of the liquid phase is lyophilized. A health functional food for hypoglycemia comprising an extract of Paeonia lactiflora. A pharmaceutical composition for preventing and treating diabetes according to claim 1, which comprises the step of extracting Paeonia lactiflora at 0 to 90 캜 with water, an alcohol having 1 to 5 carbon atoms, or a mixed solvent thereof, ≪ / RTI > A method for producing hypoglycemic health functional food according to claim 5, comprising the step of extracting Paeonia lactiflora at 0 to 90 ° C with water, an alcohol having 1 to 5 carbon atoms, or a mixed solvent thereof to prepare a peony extract ≪ / RTI >

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