KR100783205B1 - The preparation containing coriolus versicolor extract and its polysaccharides having anti-hepatocytic activity - Google Patents

Abstract

A composition comprising an extract of Coriolus versicolor and a crude polysaccharide fraction of Coriolus versicolor is provided to inhibit hepatocytic activity due to excellent antioxidative activity, thereby being usefully used as a pharmaceutical composition and a health functional food for preventing and treating liver diseases. A pharmaceutical composition for improving liver function comprises a cold water extract of Coriolus versicolor and a crude polysaccharide fraction of Coriolus versicolor as effective ingredients, wherein the weight ratio of the cold water extract of Coriolus versicolor to the crude polysaccharide fraction of Coriolus versicolor is 1:1.

Description

The preparation containing Coriolus versicolor extract and its polysaccharides having anti-hepatocytic activity}

1 is a flow chart for the manufacturing method of fingertips cold water extract.

2 is a flowchart illustrating a method for preparing a fingernail crude polysaccharide fraction.

Figure 3 is a graph showing the antioxidant activity effect of the fingerlings cold water extract and fingerlings crude polysaccharide fraction.

Figure 4 is a graph showing the hepatotoxicity inhibitory effect of the fingerlings cold water extract and fingerlings crude polysaccharide fraction.

5 is a graph showing the optimal concentration of hepatotoxicity inhibitory activity of the fingerling cold water extract and fingering crude polysaccharide fraction.

Figure 6 is a graph showing the hepatotoxicity inhibitory effect of the composition containing the fingerling cold water extract and fingering mushroom copolysaccharide fraction.

The present invention relates to a pharmaceutical composition and a dietary supplement comprising the fingering cold water extract and fingering crude polysaccharide fraction having an antioxidant activity and improving liver function as an active ingredient.

In recent years, the prevalence of various diseases has increased due to environmental pollution, the spread of new infectious diseases and immune diseases, and the increase of the elderly population. Among them, the increase in the number of adult and degenerative diseases, which frequently occur from the mid-40s, is very high. to be. Especially in Korea, metabolic and cardiovascular diseases are increasing due to the characteristics of dietary culture, stress in social life, drinking and smoking.

In Korea, cancer that occurs in multiple areas is the most important disease in Korea, but cardiovascular diseases such as heart disease and stroke, as well as metabolic diseases such as liver disease, diabetes, and obesity are becoming more serious diseases. It is becoming. On the other hand, due to the improvement of living standards according to the economic development, the interest in the health of the individual is rapidly increasing, especially in the management of adult diseases such as cardiovascular and metabolic diseases, various health maintenance methods, sports therapy, health foods and health supplements Many methods are used for prevention and treatment, such as food and preventive medicines.

Liver disease is caused by congenital factors, viral infections, or exposure to chemicals. Chronic diseases such as hepatitis B, including symptoms such as temporary and acute liver damage caused by high alcohol intake. Various outbreaks In particular, liver disease may be accompanied by symptoms such as fatty liver, cirrhosis of the liver, liver cancer, and these symptoms may cause dangerous consequences. In addition, liver disease negatively affects the function of other tissues due to deterioration of liver tissue, resulting in malnutrition, vascular disease and in some cases worsening of underlying disease.

The pathogenesis of liver disease is associated with a number of complex actions, but it is known that oxidative action in liver cells is caused by exogenous factors such as viruses and chemicals or by endogenous factors such as stress. Therefore, by ingesting a substance with high antioxidant activity, many attempts have been made to suppress liver disease by enhancing the function of hepatocytes. The most widely used food or pharmaceutical ingredients for improving liver function and treating liver disease through antioxidant activity are plant extracts and mushrooms containing a large amount of compounds of flavonoid family. Some of these materials are used for the purpose of improving liver function in folk remedies, but there are not many examples that are actually used due to lack of scientific evidence and unstable efficacy.

Coriolus versicolor is a fungus belonging to the orchard family, also called a cloud mushroom. It is a round and semicircular black or gray mushroom that grows on dead trees of coniferous and broad-leaved trees or stumps. Cloud finger mushrooms are well known for their anti-cancer activity. Especially, the functional component related to anti-cancer activity is glucan, a substance that forms the cell wall of the mushroom, and is often called polysaccharide-K (PSK). . This PSK is known to be almost nontoxic to normal cells, to show specific toxicity to cancer cells, and to activate immune cells. On the other hand, it is known that the fingering mushrooms are effective in chronic bronchitis, coronary circulation disorder, gastric ulcer, chronic hepatitis, arthritis, hypertension, but there is almost no scientific evidence.

Accordingly, the inventors have found that the cold water extract of the fingerling mushroom and the crude polysaccharide fraction of the fingerling mushroom have a high antioxidant effect and the inhibitory effect on hepatotoxicity, and the composition containing the components of the fingernail cold water extract and the fingernail crude polysaccharide fraction is liver cells. The present invention was found to be able to alleviate the toxicity and to improve liver function.

It is an object of the present invention to provide a pharmaceutical composition and a dietary supplement comprising a finger extract of cold finger mushrooms having a antioxidant activity and an effect of improving liver function, and a crude polysaccharide fraction of fingertip mushrooms as an active ingredient.

In order to achieve the above object, the present invention has an anti-oxidant activity and liver function-improving effect, including a finger extract of cold finger mushroom and crude polysaccharide fraction of finger finger mushroom as an active ingredient and a pharmaceutically acceptable carrier, excipient or diluent It provides a pharmaceutical composition.

In addition, the present invention provides a dietary supplement having antioxidant activity and liver function-improving effect including the fingerling cold water extract and the crude polysaccharide fraction of the fingerling as an active ingredient and a food supplement acceptable food supplement.

Hereinafter, the present invention will be described in detail.

The cold finger mushroom extract of the present invention is washed with water and then naturally dehydrated by washing the fingerling mushroom, and then sterile distilled water of 7 times by weight is added to extract cold water at a temperature of 4 ° C. for 72 hours, and the supernatant obtained by centrifugation is filtered through a filter paper ( 0.45 μm filter paper) was filtered to obtain sterile cold fingerling cold water extract (see FIG. 1).

On the other hand, the crude polysaccharide fraction of the fingering mushroom is put into 20 times of sterile distilled water in the washed, dehydrated and shredded fingerling mushrooms, heat-treated at 100 ℃ for 3 hours while maintaining the volume to 30% of the original volume, and extract the extract through filter paper Filtered. Ethanol (95%) was mixed in the extract to the same volume and left to stand at 4 ° C for 12 hours, to obtain a precipitate (copolysaccharide fraction) through centrifugation. The precipitate is dissolved in sterile distilled water and then heated to 80 ° C. until no ethanol odor is removed to remove ethanol. The fraction from which ethanol was removed was freeze-dried to obtain a fingerling crude polysaccharide fraction (see FIG. 2).

The pharmaceutical composition comprising the finger extract of the present invention and the crude polysaccharide fraction of the finger finger mushroom may further comprise appropriate carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical composition comprising the fingering cold water extract and fingering mushroom copolysaccharide fraction according to the present invention can be used in the form of oral forms, such as powders, granules, tablets, capsules, suspensions. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, and the like. When formulated, it is prepared using diluents or excipients such as fillers, extenders, wetting agents, and binders.

Preferred dosages of the pharmaceutical compositions of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and can be appropriately selected by those skilled in the art. Preferably, 5 mg / kg is administered daily. The frequency of administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the functional food containing the fingertips cold water extract and fingering mushroom copolysaccharide fraction may further comprise a food supplement acceptable food supplement.

The foods to which the fingerlings of the present invention can be added to the cold water extract and the fingerlings of the crude polysaccharide fraction include drinks, tea, vitamin complexes, health supplements, etc., in the form of pills, powders, granules, tablets, capsules or beverages. Can be used.

Hereinafter, the present invention will be described in detail with reference to Examples. The following Examples are presented to explain the present invention, but the present invention is not limited thereto.

<Example 1>

Preparation of fingerling mushroom cold water extract

500 g of fingerling mushrooms were washed with water and dehydrated in a certain size. The mixture was rinsed to a certain size, and 3.5 liter of sterile distilled water was added. Cold water was extracted at a temperature of 4 ° C. for 72 hours, and the supernatant obtained by centrifugation was filtered with a filter paper (0.45 μm filter paper). The freeze-drying process was carried out to obtain 55g of aseptic mushroom cold water extract.

Preparation of Crude Mushroom Polysaccharide Fractions

10 g of sterile distilled water was added to 500 g of washed, dehydrated and shredded fingernail mushrooms, heated at 100 ° C. for 3 hours while maintaining the volume at 30% of the original volume, and the extract was filtered through a filter paper (0.45 μm filter paper). To the extract, 7 liters of ethanol (95%) were mixed in the same volume and left to stand at 4 ° C for 12 hours, to obtain a precipitate (crude polysaccharide) through centrifugation. The precipitate was dissolved in 250 g of sterile distilled water and then heated to 80 ° C. until no ethanol odor was removed to remove ethanol. The ethanol-free fractions were freeze-dried to obtain 10 g of the fingerling crude polysaccharide fraction.

Example 2

Preparation of Capsule

0.05 g of the finger finger mushroom cold water extract obtained in Example 1, 0.05 g of finger finger mushroom crude polysaccharide fraction, 0.03 g of crystalline cellulose, 0.015 g of lactose, 0.005 g of magnesium stearate were mixed according to a conventional capsule preparation method and filled into gelatin capsules. To prepare a capsule.

Manufacture of tablets

The tablets were prepared by tableting 0.05g of the fingerling mushroom water extract obtained in Example 1, 0.05g of the fingerling mushroom polysaccharide fraction, 0.03g of corn starch, 0.01g of lactose, 0.005g of purified water, and 0.005g of magnesium stearate according to a conventional tablet preparation method. do.

Preparation of liquid

0.15 g of fingertip mushroom water extract obtained in Example 1, 0.15g of fingernail crude polysaccharide fraction, 0.005g of isomerized sugar, 0.005g of mannitol, and 100g of purified water were added to each component in purified water according to a conventional liquid preparation to dissolve the total 100ml. After adjusting to fill the brown bottle sterilized to prepare a liquid.

Example 3

Manufacturing of dietary supplements

0.15 g of finger finger mushroom water extract obtained in Example 1, 0.15 g of finger finger mushroom crude polysaccharide fraction, zinc oxide 0.002 g, magnesium oxide 0.005 g, magnesium stearate 0.005 g, vitamin 0.01 mg, glycerin fatty acid ester 0.005 g After mixing according to the method, to prepare a dietary supplement according to a conventional method.

Manufacture of health drinks

The fingerling mushroom water extract obtained in Example 1 0.15g, the fingerling crude polysaccharide fraction 0.15g, citric acid 0.05g, oligosaccharide 0.05g, taurine 0.05g, plum concentrate 0.002g, purified water 100g after mixing according to the conventional healthy beverage manufacturing method After stirring and heating for 2 hours, the resulting solution is filtered and obtained in a sterilized 0.5-liter container, sealed and sterilized, and then refrigerated and then used in the manufacture of a healthy beverage composition of the present invention.

Experimental Example 1

Determination of Antioxidant Activity of Cold Water Extract of Fingerling Mushroom and Crude Polysaccharide Fraction of Fingering Mushroom

In order to confirm the antioxidant activity of the cold water extract and the fingernail crude polysaccharide fraction (polysaccharide extract) of the fingerling mushroom, the cold water extract and the fingernail crude polysaccharide fraction of the fingertip mushroom prepared in Example 1 were used. picryhydarzyl (DPPH) radical scavenging activity was measured in vitro (In vitro) method to measure. DPPH is itself a very stable free radical, a purple compound that exhibits specific light absorption at 517 nm. 1 ml of DPPH solution (1.5 ⅹ 10 ^-4 M) and 0.1 ml of the finger finger mushroom cold water extract and finger finger coarse polysaccharide fractions prepared in Example 1 were mixed and left for 30 minutes, followed by a decrease in light absorption at 517 nm. Was investigated. In this experiment, BHT (butylated hydroxytoluene) was used as a positive control. By comparing the free radicals' scavenging activity by mixing the various concentrations of cold finger extracts and the finger polysaccharide fractions with DPPH solution, the ED ₅₀ (50% effective dose) values were compared. ED ₅₀ was about 12 mg / ml. On the other hand, even though the extract of the fingerlings cold water extract showed ED ₅₀ values almost equivalent to BHT as a single substance, high antioxidant activity was recognized. On the other hand, the fingernail crude polysaccharide fraction had an ED ₅₀ value of about 60 mg / ml. From the experimental results, it was confirmed that the cold water extract and the fingernail copolysaccharide fraction of the fingerling mushrooms had a certain level of high antioxidant activity. The results are shown in FIG.

Experimental Example 2

Hepatotoxicity Inhibitory Effects of Oral Mushroom Cold Water Extract and Fingernail Mushroom Polysaccharide Fraction

The inhibitory effects of hepatotoxicity on the cold water extracts of the fingerlings and the crude polysaccharide fractions of the fingerlings, which were found to have significant effects on antioxidant activity, were examined.

Female Balb / c mice (8 weeks old, 21g body weight) were prepared for the fingering mushroom cold water extract group, fingerling mushroom copolysaccharide fraction group, and 7 rats for each group as a control group. Hepatotoxicity was induced by intravenous injection of Concanavalin-A (ConA) at a dose of 10 mg / kg on day 6 after oral administration of mice once daily for 5 days. The induction of hepatotoxicity was determined by measuring the amount of GPT enzyme in the blood using a measurement kit (Dongdo Pharmaceutical). As a result of comparing and calculating relative values based on the GPT values of the normal mice, GPT values increased with time after ConA administration, and at 30 hours, the GPT value was about 2.7 times higher than that of the normal control group. As a result, mice injected with ConA intravenously after the oral administration of cold finger mushroom extract showed significantly higher hepatotoxicity inhibitory activity compared to the control group, while the crude finger polysaccharide fraction of the fingerling mushroom was lower than that of the cold finger mushroom extract. Seemed. The results are shown in FIG.

Experimental Example 3

Optimal Concentration of Hepatotoxic Inhibitory Activity by Oral Administration of Cold Water Extract of Cloudfish and Crude Polysaccharide Fraction

In order to investigate the optimal dose for the hepatotoxicity inhibitory effects of the fingerling cold water extract and the fingernail crude polysaccharide fraction, the dose of each of the cold finger mushroom extract and the fingerling crude polysaccharide fraction was Oral administration was performed for 5 days in the range of ˜10 mg / mouse, and the amount of GPT was measured from serum 30 hours after intravenous ConA injection the next day. As a result, the cold water extract and the crude polysaccharide fraction of the fingerling mushrooms did not change the hepatotoxic inhibitory effect at the amount of 5 mg / mouse or more, and it was confirmed that the dose of 5 mg / mouse was the optimal condition for the hepatotoxic inhibitory activity. The results are shown in FIG.

Experimental Example 4

Hepatotoxic Inhibitory Effects of the Compositions Containing Cold Water Extract of Cloudy Mushroom and Crude Polysaccharide Fraction of Cloudy Mushroom

In order to determine the mixing ratio of the fingerling cold water extract and the fingerling crude polysaccharide fraction that can maximize the effect of improving liver function by the composition containing the fingerling cold water extract and the fingerling crude polysaccharide fraction, it shows a high inhibitory effect on liver toxicity. The inhibitory effect on hepatotoxicity was investigated in the form of a mixture of 5 mg / mouse of cold water extract and 5 mg / mouse of crude polysaccharide fraction. As a result of the animal experiment in the same manner as in Experiment 2, it was found that the administration of a mixture of these two extracts induces a higher synergy effect than when the cold finger mushroom cold water extract and the finger finger crude crude polysaccharide fraction were administered alone. . The results are shown in FIG.

Experimental Example 5

Toxicity Test

In order to investigate the presence or absence of the toxicity of the fingerlings of the fingerlings of the fingerlings and the fingerlings of the polysaccharide fraction of Example 1 by oral administration to rats, 1g / kg, 2g / kg and 5g / kg Five oral single doses of dogs were observed for 10 days of death, general symptoms, body weight change, and autopsy findings. As a result, there were no deaths during the entire test period in all the administration groups, and no change in general symptoms observed compared to the control group. In addition, few symptoms were observed in the anatomical findings at the time of wormworm and autopsy.

As described above, the composition of the present invention can be useful as a pharmaceutical composition and health functional food for the prevention and treatment of liver disease because it has an excellent effect on improving liver function by inhibiting liver toxicity due to excellent antioxidant activity.

Claims (3)

A finger composition for improving liver function, characterized in that the fingerling cold water extract and the fingernail crude polysaccharide fraction as an active ingredient. The method of claim 1, The fingering cold water extract and fingering mushroom copolysaccharide fractions of the fingerlings, characterized in that the liver is mixed in a weight ratio of 1: 1. A healthy functional food for improving liver function, which is characterized by the fingerling cold water extract and the fingernail crude polysaccharide fraction.

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