KR20200047956A - Sample change detection device and sample change detection method - Google Patents

Abstract

The present invention is intended to provide an equipment capable of replacing an expensive spectrometer used for detecting changes in color and brightness. Disclosed is a sample change sensing apparatus. The sample change sensing apparatus according to the present invention may comprise: a light source unit including a light source; a sample unit positioned on an optical path by the light source, wherein a sample before a chemical reaction or a sample after the chemical reaction is disposed; a measurement unit measuring wavelength characteristics of light passing through the sample unit by including at least two light receiving elements capable of measuring a specific wavelength band having different peak values; and a detection circuit unit which senses the changes in color and brightness of the sample by using results measured by the measurement unit. The sample change sensing apparatus may further include a beam splitter uniformly transmitting the light passing through the sample unit to each light receiving element of the measurement unit by being positioned between the sample unit and the measurement unit.

Description

Sample change detection device and sample change detection method

The present invention relates to an apparatus capable of detecting a sample change and a method for detecting a sample change.

In hospitals, for qualitative / quantitative detection of specific pathogens in body fluids, samples that change color depending on the presence or absence of pathogens or samples that change brightness depending on the concentration of pathogens are frequently used. In the chemical field and the bio field, which are recently studied, there are many cases in which a sample reacts to change color or brightness. Whether or not the color of the sample has changed is currently mostly checked with the naked eye, which is inaccurate. Also, it is difficult to visually check similar colors. Most of the verification that the color or brightness of the sample has changed is using an ELISA reader or a spectrometer device, which is large, heavy, expensive, and checks and analyzes the measurement results using a computer, so it is used in medical or experimental sites. It is difficult to apply to real-time diagnosis.

In the present invention, it is intended to provide a device capable of replacing an expensive spectrometer used for detecting changes in color and brightness.

In addition, in the present invention, it is not limited to the type of the sample, and it is intended to provide a device capable of detecting a change in color and brightness of the sample.

The problems to be solved by the present invention are not limited to the problems mentioned above. Other technical problems not mentioned will be clearly understood by those skilled in the art from the following description.

The sample change detection device according to the present invention includes: a light source unit including a light source; A sample portion positioned on the optical path by the light source, and in which the sample before the chemical reaction or the sample after the chemical reaction is disposed; A measuring unit measuring at least two light-receiving elements capable of measuring a specific wavelength band having different peak values, and measuring characteristics of light passing through the sample unit; A detection circuit unit that detects a change in color and brightness of the sample using the results measured by the measurement unit; It may include.

The sample change detection device may further include a beam splitter positioned between the sample part and the measurement part to uniformly transmit light passing through the sample part to each light receiving element of the measurement part.

The sample change detection device may provide one optical path in the direction of the light source unit based on the sample unit, and an optical path in the direction of the measurement unit as many as the number of light receiving elements included in the measurement unit.

The inner surface of the sample change detection device is formed of a material capable of minimizing the loss generated while the light of the light source unit travels along the path, and the outer surface of the sample change detection device receives an external light signal. It may be formed of a material for preventing it from transmitting into the sample change detection device.

The detection circuit portion, amplifying circuit portion for amplifying the signal obtained from the measurement portion; A conversion circuit unit converting the amplified signal into a digital signal; It may include a; calculation unit for determining a change in the sample by calculating the converted signal.

The calculation unit, the magnitude relationship of at least two output values generated as a result of the sample passing through each of the at least two light-receiving elements before the chemical reaction, and the result of the sample passing through each of the at least two light-receiving elements after the chemical reaction It is possible to compare the magnitude relationship of at least two output values generated by.

The calculation unit, if the magnitude relationship between at least two output values generated for the sample before the chemical reaction and the at least two output values generated for the sample after the chemical reaction is changed, the color is changed, the magnitude relationship If there is no change, it can be determined that the brightness has changed.

Sample change detection device according to an embodiment of the present invention, a display unit for outputting a change detection result of the sample; It may further include.

A method of detecting a sample change according to another embodiment of the present invention includes: irradiating a light source with a sample before reaction; Measuring at least two output values of the first light by at least two light receiving elements capable of measuring a specific wavelength band having a different peak value for the first light passing through the sample; Irradiating the light source to the sample after reaction; Measuring at least two output values of the second light by at least two light receiving elements capable of measuring a specific wavelength band having a different peak value for the second light passing through the sample after the reaction; And comparing the magnitude relation between at least two output values of the first light and the magnitude relation between the at least two output values of the second light to determine a change result of the sample.

In the sample change detection method, comparing the magnitude relationship between at least two output values of the first light and the magnitude relationship between the at least two output values of the second light may include comparing the magnitude relationship of the first light by the at least two light receiving elements. Comparing the first step; A second step of comparing the magnitude of the second light by the at least two light receiving elements; The first step and the third step of determining whether to reverse the relationship between the second step; may further include.

The method for detecting a sample change according to the present invention may further include outputting that the color has been changed when the relationship between the first and second steps is reversed.

The method for detecting a sample change according to the present invention may further include outputting that the brightness is changed when the relationship between the first and second steps is not reversed.

According to the present invention, it is possible to provide equipment that can replace the spectrometer used to detect color and brightness changes.

According to another effect of the present invention, it is economical because it can be miniaturized at a low cost while having a similar level of detection accuracy compared to a spectrometer used for a similar purpose.

According to another effect of the present invention, since the type of the detectable sample is not limited, equipment applicable to various fields can be provided.

The effects of the present invention are not limited to the effects described above. Effects not mentioned will be clearly understood by those skilled in the art from the present specification and the accompanying drawings.

1 is a view for explaining the detection principle of the sample change detection device according to the present invention.
2 is a block diagram showing a sample change detection device according to the present invention in a block diagram.
3 is a block diagram showing the configuration of the detection circuit unit according to FIG. 2.
4 is a view showing an embodiment of a sample change detection device according to the present invention.
5 is a view for explaining a determination method in a sample change detection device according to the present invention
6 is a flowchart illustrating a sample change detection method according to the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. In addition, in the detailed description of the preferred embodiment of the present invention, when it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted. In addition, the same reference numerals are used throughout the drawings for parts having similar functions and functions.

'Including' a component means that other components may be included, not excluded, unless otherwise stated. Specifically, the terms “include” or “have” are intended to indicate that a feature, number, step, action, component, part, or combination thereof described in the specification exists, or that one or more other features or It should be understood that the presence or addition possibilities of numbers, steps, actions, components, parts or combinations thereof are not excluded in advance.

Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from other components. For example, the first component may be referred to as a second component without departing from the scope of the present invention, and similarly, the second component may be referred to as a first component.

Singular expressions include plural expressions unless the context clearly indicates otherwise. In addition, the shape and size of elements in the drawings may be exaggerated for a more clear description.

The '~ unit' and '~ module' used throughout this specification are units for processing at least one function or operation, and may mean hardware components such as software, FPGAs, or ASICs. However, '~ unit' and '~ module' are not limited to software or hardware. The '~ unit' and '~ module' may be configured to be in an addressable storage medium, or may be configured to reproduce one or more processors.

As an example, '~ unit' and '~ module' are components such as software components, object-oriented software components, class components and task components, processes, functions, attributes, It may include procedures, subroutines, segments of program code, drivers, firmware, microcode, circuitry, data, database, data structures, tables, arrays and variables. Components and functions provided by '~ unit' and '~ module' may be performed separately by a plurality of components and '~ unit' and '~ module', or may be integrated with other additional components. .

The present invention proposes a sample change detection device and a sample change detection method that can easily and economically discriminate changes in color and brightness of a sample due to chemical, bio, and biological reactions. In the present invention, a light-receiving element having a wavelength band optimized for changes in a spectrum before and after reaction of a target sample is selected, and light passing through the sample before and after the reaction is measured with a plurality of light-receiving elements to compare and analyze an output value. When the magnitude relationship between the intensity values of the output light is changed, it is possible to determine that the color is changed by moving the wavelength of the sample, and if the output is increased or decreased without changing the magnitude relationship, it can be determined that the brightness of the sample is changed. have. Using such a detection device and a detection method, it is possible to check in real time the change in color and brightness and the change in fluorescence as the sample reacts and the component changes or the concentration changes. In addition, it is possible to detect accurate color and brightness changes with a smaller amount of samples than the existing spectrometer device. It is expected that the system configuration is simple and the price of parts is low, so it has high mass productivity and great marketability.

What is described below is only an embodiment of the present invention, and is not limited to the parts disclosed in the drawings and description of the invention. Hereinafter, a technical concept and an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

1 is a view for explaining the detection principle of the sample change detection device according to the present invention. The conceptual feature of the present invention uses at least two light-receiving elements having sensitive responsiveness in light of two wavelengths having the largest difference in absorbance and transmittance. By using the at least two light-receiving elements, the output values of the sample before the reaction and the sample after the reaction, which may have two different colors or have differences in concentration, are measured, and whether the color is changed or the density is changed through the inversion of the output value. Is to detect. This is because the difference in wavelength occurs when the color of the sample changes before and after the reaction, and as a result, the relationship between the output values generated as a result of passing through the light-receiving element having a defined wavelength band will be reversed.

Referring to FIG. 1, a light receiving element capable of measuring two peak wavelength bands is provided. The light-receiving element may be a device that generates electrical output according to the intensity of light reaching. The light-receiving device indicated in red can measure a peak wavelength of 365 nm, and the light-receiving device indicated in blue can measure a peak wavelength of 525 nm. Each light-receiving device capable of measuring the peak wavelength band can detect a wavelength band in the periphery of the corresponding peak wavelength value. When light having a wavelength within a range detectable by a light-receiving element comes in, the light-receiving element can measure it to produce a corresponding output value, and when light having a wavelength outside a range that can be measured by a light-receiving element comes in, it can produce an output value. none.

It is assumed that light is irradiated to each of the light-receiving elements. If the intensity distribution of light passing through the sample is equal to A, the output of the light receiving element capable of measuring the peak wavelength band at 365 nm will be greater than the output of the light receiving element capable of measuring the peak wavelength band at 525 nm. . Conversely, if the intensity distribution of light passing through the sample is equal to B, the output size is the output of the light-receiving device capable of measuring the peak wavelength band at 525nm and the output size of the light-receiving device capable of measuring the peak wavelength band at 365nm. Will appear larger than that. That is, it is possible to easily discriminate the change in the brightness or color of the sample by using light-receiving elements having different wavelength bands.

2 is a block diagram showing a sample change detection device according to the present invention in a block diagram.

The sample change detection device 1 according to the present invention may include a light source unit 10, a sample unit 20, a measurement unit 30, a detection circuit unit 40 and a display unit 50.

The light source unit 10 may include a light source 11 that emits light. The light source unit 10 may further include a light source driving circuit 12 that drives the light source 11. The light source 10 may be an LED light source. The light source may be a light source that knows light intensity by wavelength. The light source may be a light source having a brightness peak value at one or more wavelengths in a wavelength band of the visible light region. Alternatively, the light source may be a white light source and emit white light. The light source unit 10 may be connected to one end of the sample change detection device 1.

The sample 21 may be fixed to the sample unit 20. The sample portion 20 may be located on the optical path of light generated by the light source 11. The fixed sample may be a sample before a chemical reaction. Alternatively, the fixed sample may be a sample after a chemical reaction. The sample may be a colored sample or an intangible sample. The sample unit 20 may further include a fixing device for fixing the sample so that the position of the colored or intangible sample does not change the optical path during the measurement process. The light may pass through the sample portion 20 and pass through the sample 21 fixed to the sample portion 20. The sample part 20 may be located between the path of the light source part 10 and the measurement part 30.

The measurement unit 30 may include at least two light-receiving elements having different responsive peak wavelengths within a wavelength band of the visible light region. At least two light-receiving elements may detect a specific wavelength band having a different peak value. An example of a specific wavelength band having different peak values of the at least two light receiving elements may be two wavelength bands having different colors in the visible light band. The wavelength band of at least two light-receiving elements may be a wavelength of a red region in the visible light band and a wavelength of a blue region in the visible light band. When three or more light-receiving elements are provided, the wavelength bands that can be measured by three or more light-receiving elements may be wavelength bands for measuring different color regions. However, depending on the wavelength band of the sample to be measured, the wavelength band of the light receiving element may be selected as a wavelength band capable of measuring the color of the same region while having different peak wavelength values. The light to be measured by the measurement unit 30 may be light transmitted through the sample unit 20. At least two light-receiving elements included in the measurement unit 30 may measure light intensity of a specific wavelength transmitted through the sample of the sample unit 20. The unit of intensity of light may be watts (W). The light-receiving elements of the measurement unit 30 may be connected to the ends of each path to detect different wavelength bands. The light-receiving element can detect light transmitted through the sample. In at least two light receiving elements included in the measurement unit 30, a range of wavelengths that can be received through the light receiving elements may be determined in advance. The range of wavelengths that can be received through the light-receiving element can be determined using a color filter.

The measurement unit 30 may include at least two light receiving elements. Two or more light-receiving elements included in the measuring unit may be provided, and the number of light-receiving elements and the number of output wavelengths may be formed in correspondence. For example, when there are two light-receiving elements included in the measurement unit, light transmitted by irradiating light to a sample before the reaction passes through the two light-receiving elements, so that each of the light-receiving elements can measure 2 wavelengths. Outputs may appear. In addition, the light transmitted by irradiating the sample after the reaction passes through the two light-receiving elements, so that each of the two light-receiving elements may have two output results in a wavelength band that can be measured.

That is, in the case of a measuring unit including two light receiving elements, a total of four results are obtained: two output values of reaction results of two light receiving elements for a sample before reaction and two output values of reaction results of two light receiving elements for a sample after reaction. Can be.

If three light-receiving elements are included, the reaction result output values for three light-receiving elements having different measurable wavelength bands for the sample before the reaction and the reaction result output values for the three light-receiving elements for the sample after the reaction A total of six results can be derived from three.

The detection circuit unit 40 may detect a change in color and brightness of the sample using the result measured by the measurement unit 30. The detailed structure of the detection circuit 40 will be described later in the description of FIG. 3 below. The detection circuit unit 40 may be connected to the measurement unit 30 to detect a sample change through an output value measured by the measurement unit 30.

The display unit 50 may output the result of the detection circuit unit 40.

3 is a block diagram showing the configuration of the detection circuit unit 40 according to FIG. 2. The detection circuit unit 40 may further include an amplification circuit unit 41, a conversion circuit unit 42, and a calculation unit 43. The amplifying circuit portion 41 can amplify the signal obtained by the measuring portion 30. The amplifying circuit unit 41 may be configured as a circuit capable of amplifying the output signal of the light receiving element of the measuring unit 30. The conversion circuit unit 42 may convert the signal amplified by the amplification circuit unit 41 into a digital signal. The conversion circuit 42 may convert an analog signal to a digital signal. The calculation unit 43 may calculate a sample change by calculating the converted digital signal.

The calculation unit 43 has a magnitude relationship between at least two output values generated as a result of the sample passing through each of the at least two light receiving elements before the chemical reaction, and the sample after the chemical reaction passing through each of the at least two light receiving elements. It is possible to compare the magnitude relationship of at least two output values generated as a result. That is, the calculation unit 43 may measure samples before and after the reaction in different wavelength bands, and use the measured values to determine a change in the composition and concentration of the detection substance in the sample. If there is no change in the magnitude relationship between the measured output values before and after the reaction for each wavelength band, it can be determined that the concentration of the detection substance in the sample is changed, and if the relationship between the magnitude and the magnitude of the detection substance in the sample is changed. If there is no change in the magnitude relationship between the measured output values, at least two output values generated as a result of the sample before the reaction passing through each of the at least two light receiving elements and the sample after the reaction pass through each of the at least two light receiving elements. There may be cases where at least two output values generated as a result are completely identical. When the sample is completely identical, it may mean that the sample before the reaction and the sample after the reaction are not different.

4 is a view showing an embodiment of a sample change detection device 1 according to the present invention. According to FIG. 4, the sample change detection device 1 may be formed in the shape of a slingshot. According to another embodiment of the present invention, the overall shape of the sample change detection device 1 may vary according to the number of light receiving elements included in the measurement unit 30. The sample change detection device 1 illustrated in FIG. 4 illustrates a case in which two light receiving elements 31 and 32 are included.

The sample change detection device 1 may be provided in a sealed interior. The inner surface of the sample change detection device 1 may be formed of a material capable of minimizing the loss generated while the light of the light source unit 10 progresses along the path. The outer surface of the sample change detection device 1 may be formed of a material for preventing external light signals from being transmitted into the sample change detection device 1. That is, the sample change detection device 1 does not receive any influence from the outside when the light source 11 is irradiated to the sample 21, and the surface is treated and formed so as to improve accuracy by preventing light from being lost even inside. Can be. The sample change detection device 1 may be configured to maintain a constant light path and block ambient noise signals. The sample change detection device 1 can create an optimal light path condition and a dark room environment.

The sample change detection device 1 may further include a beam splitter 60. The beam splitter 60 is located between the sample part 20 and the measurement part 30 and uniformly transmits light passing through the sample part 20 to each light receiving element of the measurement part 30. It can be done. The beam splitter 60 may split the light transmitted through the sample in two or more paths to equally match the intensity of light reaching each light receiving element.

 According to another embodiment of the sample change detection device 1, when the beam splitter 60 is not included, it is possible to compare the relationship between light and small by irradiating light to light receiving elements having different wavelength bands with a time difference.

However, in the case of using the beam splitter 60 in the sample change detection device 1, since light can be distributed at the same intensity within the same time period, at least two light receiving elements can be used at the same time, which can be much more efficient in terms of time.

In the sample change detection device 1 according to another embodiment of the present invention, the step of measuring the sample before the reaction may be omitted. In the sample change detection device 1, there may be a sample sample before the reaction, and a result output for each wavelength band by irradiating light to the sample sample before the reaction may be stored in the detection circuit unit. According to another embodiment of the present invention, if there is no need to compare the magnitude of the result by irradiating light to the sample before the reaction and the sample after the reaction, the sample after the reaction is already stored when the result of the light irradiation is already stored in the sample before the reaction Since it is only necessary to compare the relationship between light and light by irradiating light, it may be efficient in terms of time.

The light source unit 10, the sample unit 20, the measurement unit 30, and the detection circuit unit 40 included in the sample change detection device 1 may be arranged and formed in order. In addition, although the display unit 50 is shown in the form of a block diagram, it is formed on the outer surface of the sample change detection device 1 to output a result of detecting a change in color or brightness of the sample. The display unit 50 may be electrically connected to the detection circuit unit 40 to output results in real time.

The sample fixed to the sample portion 20 may be formed by being contained in any container. The sample fixing unit 22 is disposed between the light source unit 10 and the measurement unit 30 to fix the sample. The sample fixing unit 22 may be disposed between the light source unit 10 and the beam splitter 60 to fix the sample. The light transmitted from the sample unit 20 may reach the measurement unit 30 through the beam splitter 60. The intensity of light passing through the beam splitter 60 may be the same.

The intensity of light transmitted to the light receiving elements 31 and 32 included in the measurement unit 30 may be the same. The peak wavelength bands that can be measured by the respective light receiving elements 31 and 32 may have different values. The measurement unit 30 may be located at one end of the sample change detection device 1. The distance between the beam splitter 60 and each light-receiving element 31 and 32 may be formed identically. Through each of the light receiving elements 31 and 32, a sample before reaction and a measured value of the sample after reaction can be amplified, converted, and calculated through a detection circuit unit 40 connected to the measurement unit 30.

According to another embodiment of the present invention, when the light source 11 is blocked by the sample change detection device 1, it is also possible to detect a color change and an intensity change of a sample that exhibits fluorescence.

5 is a view for explaining a determination method in a sample change detection device according to the present invention.

According to FIG. 5, in the sample change detection device according to the present invention, a detection process in terms of spectrum is described.

FIG. 5 (a) is a diagram for explaining when light is transmitted through a sample before reaction, and FIG. 5 (b) is a diagram for explaining when light is transmitted through a sample after reaction.

과

는 적어도 2개의 수광소자에서 측정 가능한 빛의 피크 파장을 의미한다. 상기

과 상기

의 파장은 동일한 값을 가지지 아니하도록 구성된다. 시료의 파장 별 흡광도에 따라서 시료를 통과한 이후 적어도 2개의 수광소자로 들어가는 빛의 밝기는 차이가 있게 된다. 도 5(a)에 따르면 반응 전의 시료가 고정된 시료부(20)를 통과한 광원의 출력값은,

에서의 광의 세기가

에서의 광의 세기보다 크게 출력되는 것을 확인할 수 있다. 도 5(b)에 따르면, 반응 후의 시료가 고정된 시료부를 통과한 광원의 출력값은,

에서의 광의 세기가

에서의 광의 세기보다 크게 출력되는 것을 확인할 수 있다. 도 5에 나타나 있듯이, 반응 전의 시료와 반응 후의 시료의 파장 별 흡광도에 있어서 차이가 발생한 것을 확인할 수 있다.According to FIGS. 5 (a) and 5 (b), since the light sources used in the light source unit 10 are the same, it can be confirmed that the brightness of each light source is the same. In the sample change detection device 1, the light source uses the same wavelength. 5 (a) and 5 (b), it can be seen that there is a difference in absorbance for each wavelength of the sample. Expressed on the sample part

and

Means a peak wavelength of light that can be measured by at least two light receiving elements. remind

And remind

The wavelength of is configured not to have the same value. Depending on the absorbance for each wavelength of the sample, there is a difference in brightness of light entering the at least two light receiving elements after passing through the sample. According to FIG. 5 (a), the output value of the light source that has passed through the sample portion 20 where the sample before the reaction is fixed is

The intensity of light in

It can be seen that the output is greater than the intensity of light at. According to Figure 5 (b), the output value of the light source through the sample portion is fixed after the reaction sample,

The intensity of light in

It can be seen that the output is greater than the intensity of light at. As shown in FIG. 5, it can be confirmed that a difference occurred in absorbance for each wavelength of the sample before the reaction and the sample after the reaction.

5 is a view for explaining the detection process of the present invention to be easy to understand, and the absorbance for each wavelength of the sample before and after the reaction may or may not vary depending on the degree of reaction. 5 corresponds to a diagram showing a result of changing the absorbance for each wavelength of the sample because the color of the sample has changed.

5 corresponds to an embodiment when two light receiving elements are used. If three or more light-receiving elements are used, the magnitude of the three output values of the sample before the reaction through the three light-receiving elements and the magnitude of the three output values of the sample after the reaction through the three light-receiving elements are used. You can compare whether or not the relationship is reversed. When a plurality of light-receiving elements are used as described above, since a plurality of comparison results can be obtained compared to a case where two light-receiving elements are used, a more accurate result can be obtained. In addition, even when three or more light-receiving elements are used, beam splitters can be used to split light in three or more paths so that they can be applied with the same intensity.

6 is a flowchart illustrating a sample change detection method according to the present invention.

In the sample change detection method according to the present invention, the light source is irradiated to the samples before and after the reaction, respectively. At this time, the light source may be white light. The light source irradiated to the sample before the reaction is measured through at least two light receiving elements capable of measuring different wavelength bands. The light source irradiated to the sample after the reaction is measured through at least two light-receiving elements capable of measuring different wavelength bands. As a result of the measurement, at least two output values of the light intensity for the sample before the reaction are measured, and at least two output values of the light intensity for the sample after the reaction are measured. The magnitude relationship of at least two output values measured for the sample before the reaction is compared. In addition, the magnitude relationship of at least two output values measured for the sample after the reaction is compared. It is determined whether the relationship of the wavelength value in the sample after the reaction is reversed compared to the relationship of the output value of the sample before the reaction. At this time, if the relationship between the case and the case is inverted, the sample reacts and the color is changed to determine that the component is changed, and the corresponding result is output through the display unit. If the relationship between the large and the small size is not inverted, the sample reacts and only the brightness is changed to determine that the concentration of the sample is changed, and the corresponding result is output through the display unit.

Hereinafter, description will be made using specific values for easy understanding. If the output value measured by the sample before reaction is assumed to be 250 mW measured by the first light-receiving element and 550 mW measured by the second light-receiving element. At this time, it can be seen that the output value measured through the second light receiving element has a larger value than the output value measured through the first light receiving element. It is assumed that the output value measured by the sample after the reaction is 300 mW measured by the first light-receiving element and 600 mW measured by the second light-receiving element. At this time, it can be seen that the output value measured through the second light receiving element has a larger value than the output value measured through the first light receiving element.

That is, in the case of the sample before the reaction and the sample after the reaction, since the output value measured through the second light-receiving element has a larger value than the output value measured through the first light-receiving element, the magnitude between the output values measured by the light-receiving element is large. There is no reversal of relationship. Therefore, in this case, it can be confirmed that only changes in brightness were made through the reaction of the sample, and it was found that there was a change in the concentration of the sample. Conversely, when the sample after the reaction is measured so that the output value measured through the first light-receiving element has a larger value than the output value measured through the second light-receiving element, there is a reversal of the relationship between the output values measured by the light-receiving element. Therefore, it can be seen that the color of the sample changed through the reaction, and there was a change in the composition of the sample.

The sample change detection device 1 according to the present invention is capable of detecting color changes according to changes in the composition and concentration of a sample in any material such as ions, polymers, DNA, and bacteria. In addition, the sample change detection device 1 according to the present invention is capable of detecting not only a change in color, but also a change in optical intensity of a sample.

Therefore, according to the present invention, it is expected that it can be applied to various fields as a chemical sensor and a biosensor having low price, high accuracy, and even portability. In addition, the present invention is expected to be useful for real-time diagnosis in the medical field.

It is to be understood that the above embodiments are presented to help the understanding of the present invention, and do not limit the scope of the present invention, from which various deformable embodiments belong to the scope of the present invention. The technical protection scope of the present invention should be determined by the technical spirit of the claims, and the technical protection scope of the present invention is not limited to the literary description of the claims, but is a category in which technical value is substantially equal. It should be understood that it extends to the invention.

1: Sample change detection device
10: light source unit
20: sample part
30: measuring unit
40: detection circuit
50: display unit
60: beam splitter

Claims (12)

A light source unit including a light source;
A sample portion positioned on the optical path by the light source, and in which the sample before the chemical reaction or the sample after the chemical reaction is disposed;
A measuring unit measuring at least two light-receiving elements capable of measuring a specific wavelength band having different peak values, and measuring characteristics of light passing through the sample unit;
A detection circuit unit that detects a change in color and brightness of the sample using the results measured by the measurement unit; Sample change detection device comprising a. According to claim 1,
A sample change detection device further comprising a beam splitter positioned between the sample part and the measurement part to uniformly transmit light passing through the sample part to each light receiving element of the measurement part. According to claim 1,
The sample change detection apparatus, characterized in that the optical path in the direction of the light source unit with respect to the sample unit is provided as one, and the optical path in the direction of the measurement unit is provided by the number of light receiving elements included in the measurement unit. Sample change detection device. According to claim 1,
The inner surface of the sample change detection device is formed of a material capable of minimizing the loss that occurs while the light of the light source unit travels along the path,
The outer surface of the sample change detection device, the sample change detection device characterized in that it is formed of a material for preventing the external light signal to pass through the sample change detection device. The method according to any one of claims 1 to 4,
The detection circuit unit,
An amplifying circuit portion for amplifying the signal obtained from the measuring portion;
A conversion circuit unit converting the amplified signal into a digital signal;
A sample change detection device comprising; a calculation unit for determining a change in the sample by calculating the converted signal. The method of claim 5,
The calculation unit,
The magnitude relationship of at least two output values generated as a result of the sample passing through each of the at least two light receiving elements before the chemical reaction,
A sample change detection device for comparing the magnitude relationship of at least two output values generated as a result of the sample passing through each of the at least two light receiving elements after a chemical reaction. The method of claim 6,
The calculation unit,
If there is a change in the magnitude relationship between at least two output values generated for the sample before the chemical reaction and the at least two output values generated for the sample after the chemical reaction, the color is changed, and the magnitude relationship is not changed. In case the sample change detection device determines that the brightness has changed. The method of claim 7,
A display unit that outputs a change detection result of the sample; Sample change detection device further comprising a. Irradiating a light source with a sample before reaction;
Measuring at least two output values of the first light by at least two light receiving elements capable of measuring a specific wavelength band having a different peak value for the first light passing through the sample;
Irradiating the light source to the sample after reaction;
Measuring at least two output values of the second light by at least two light receiving elements capable of measuring a specific wavelength band having a different peak value for the second light passing through the sample after the reaction;
And comparing the magnitude relation between at least two output values of the first light and the magnitude relation between the at least two output values of the second light to determine a change result of the sample. The method of claim 9,
Comparing the magnitude relationship between the at least two output values of the first light and the magnitude relationship between the at least two output values of the second light,
A first step of comparing the relationship between the first and second light sources by the at least two light receiving elements;
A second step of comparing the magnitude of the second light by the at least two light receiving elements;
And a third step of determining whether reversal of the magnitude relationship in the first step and the second step is further included. The method of claim 10,
And outputting that the color has changed when the relationship between the first and second stages is reversed. The method of claim 10,
And outputting that the color has changed when the relationship between the first and second stages is reversed.

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