KR20200034217A - Composition for improving atopic skin of fermented product of colostrum - Google Patents

Abstract

The present invention relates to an anti-atopy composition with a fermented product of colostrum. More specifically, the present invention relates to an anti-atopy composition in which a fermented product obtained by fermenting, as a new use of the fermented product of colostrum obtained by fermenting colostrum, colostrum using fermenting bacteria such as lactic acid bacteria can be used as a pharmaceutical composition used for the treatment and prevention or a cosmetic composition used for ameliorating atopy skin such as atopy and psoriasis.

Description

Composition for improving atopic skin of fermented product of colostrum

The present invention relates to a composition for the anti-atopy of the fermented product of colostrum, and more specifically, as a new use of the fermented product of colostrum obtained by fermenting colostrum, atopic fermentation product obtained by fermenting colostrum with fermentation bacteria such as lactic acid bacteria And an atopic composition that can be used as a cosmetic composition used to improve atopic skin such as psoriasis or a pharmaceutical composition used for treatment and prevention.

The largest organ in humans is the skin. The skin is composed of epidermis and dermis. The epidermis constantly divides the cells, creating several layers. At this time, the outermost cells die and become hard, which is one of the best defenses to protect the body from external stress, and bacteria cannot penetrate the skin.

Because the skin has sebaceous glands and sweat glands, the sebaceous glands secrete sebum, and the sweat glands secrete moisture to create a sebaceous membrane and protect the stratum corneum, which is why human skin is always moist, shiny and smooth. It becomes possible.

In the case of humans, when the moisture content of this stratum corneum reaches 20%, it is the most ideal skin, and the beauty stands out. However, when the water content falls below 10%, the keratin to protect the skin cannot be in place and falls off or opens in between, making it easier to penetrate foreign substances or bacteria, causing inflammation, diseases, allergies, etc. The probability of this occurring increases. Also, the metabolism is worsened because the metabolism cannot be smoothly performed. Moreover, people with sensitive skin are on the rise due to natural pollution, ultraviolet rays, and indoor environments. In the case of such sensitive skin, side effects occur when using a popular skin cosmetic composition, and erythema, itching, itchiness, and severe inflammation occur.

Cosmetics are intended to protect the skin from the outside, to dress and clean it, but if you look closely at the composition of the cosmetics, the ingredients essential for the formation of the product, unlike the original meaning, enter, so that the ingredients can irritate the skin. have. Examples include emulsifiers, fragrances and preservatives. Since these are mostly made by chemical reaction synthesis, in the case of sensitive skin, the probability of accompanying inflammatory reactions, allergies, and erythema increases.

Lactobacillus refers to bacteria that break down carbohydrates into lactic acid by metabolism. When the amount of lactic acid increases, the acidity decreases and lactic acid bacteria die by themselves. Therefore, fermentation time is important, and making it resistant to oxidative stress is also a method.

Lactic acid produced by lactic acid bacteria plays a huge role. It has anti-oxidant, anti-cancer, and anti-radioactive properties, and also has anti-inflammatory effects. In addition, it also has the ability to increase the water bond of the stratum corneum, improving the fine wrinkles of the skin and softening the skin.

Interleukin 1 (IL-1) is a type of cytokine that mediates inflammatory reactions and is produced by several cells, such as activated monocytes, epithelial cells, or vascular endothelial cells. Another representative inflammatory component is cytokine tumor necrosis factor-α (TNF-α).

Conversely, there are also cytokines that reduce the inflammatory response, Interleukin-10. In the case of Interleukin-10, it plays a role in suppressing the expression of enzymes related to inflammation such as inducible nitric oxide synthase (iNOS) and inducible cyclooxyganase (COX-2) present in macrophages (Niiro, Hiroaki, et al, 1995) )

Lactoferrin is present in the existing colostrum, so it has an anti-inflammatory effect. In the case of lactoferrin, it is well known that it plays a role in the regulation of Tumor Necrosis Factor-α (TNF-α) or Interleukin1 (IL-1) (Yamanaka, 2003).

In addition, colostrum contains Insulin-like Growth Factor-1 (IGF-1), which is known to promote overall growth and has the ability to regulate cell growth and amplification (Uruakpa et al. , 2002).

Casein contained in colostrum accounts for 3% of the total milk component and about 80% of the milk protein, and has been found to cause allergies to the body when ingested (G. H. Docena et al, 1996). In addition, casein is used as a cosmetic ingredient, but research has not been sufficiently conducted.

In addition, colostrum contains exfoliating ingredients (Kwon Yoo-bin, et al. 2007), and is also known to contain TGF-beta, which inhibits melanin pigments and causes a whitening effect (Martinez-Esparza M, et al) , 1997), and colostrum contains a lot of proven hyaluronic acid with excellent moisturizing effect (Pedersen, et al, 2014).

Conventionally, as a cosmetic composition using colostrum, Korean Patent No. 10-0964831 proposes a cosmetic composition for promoting keratin differentiation of skin containing colostrum as an active ingredient, wherein colostrum components have anti-inflammatory, anti-allergic, and moisturizing effects. It explains various usefulness of cosmetics application, including the efficacy of promoting colostrum differentiation of colostrum ingredients.

In addition, in Korean Patent No. 10-1003740, the new Streptococcus thermophilus strain and fermented colostrum probiotics for pigs using the same improve the growth rate of livestock, and proliferation of harmful microorganisms in the intestine such as Escherichia coli and Salmonella. It has been proposed to prevent bacterial diarrhea disease by suppressing and to reduce the use of antibiotics and improve productivity by suppressing the death of livestock. In addition, in Korean Patent Publication No. 10-2002-0021908, colostrum is a health supplement food fermented using lactic acid bacteria, which reduces the amount of harmful microorganisms present in colostrum by fermentation method using lactic acid bacteria, and uses various functional substances possessed by colostrum. Suggestions are made about supplements that can be used as is.

In addition, Korean Patent Registration No. 10-0619289 discloses a method for manufacturing whitening cosmetics in which colostrum albumin is associated with arbutin, a whitening agent, and Korean Patent Publication No. 10-2009-0071998 discloses skin exfoliation using colostrum as an active ingredient. A composition for promoting differentiation has been proposed, and US Patent No. 5750149 proposes a technique for cosmetics containing substances derived from colostrum of horses.

In the above patent invention, a technique of fermenting lactic acid bacteria of some colostrum is known, and it proposes various effects of colostrum components, a whitening effect by arbutin, and an absorption effect of colostrum's immune elements into the skin.

However, in particular, it is not considered effective to improve atopic skin, and in reality, no cosmetics or medicines have been proposed that show a proper improvement effect of atopic skin without side effects.

Korean Registered Patent No. 10-0964831 Korean Registered Patent No. 10-1003740 Korean Patent Publication No. 10-2002-0021908 Korean Registered Patent No. 10-0619289 Korean Patent Publication No. 10-2009-0071998 U.S. Patent No. 5,750,149

The present invention is to solve the problems of the prior art as described above and to provide a new use for the fermentation product obtained by fermenting colostrum components based on the results of studying the utilization of colostrum as a solution.

Therefore, an object of the present invention is to provide a use for improving the anti-atopy of the colostrum fermented products fermented colostrum.

In addition, another object of the present invention is more specifically to provide a cosmetic composition for moisturizing and anti-atopic for aerobic fermentation products of colostrum.

In addition, another object of the present invention is to provide a pharmaceutical composition for improving atopic skin against aerobic fermentation products of colostrum more specifically.

To solve the above problems, the present invention provides an anti-atopic composition containing fermented products fermented with colostrum as an active ingredient.

As a preferred embodiment of the present invention, colostrum contains an anti-atopic composition containing the aerobic fermentation product of colostrum fermented under aerobic conditions as an active ingredient.

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum can be preferably applied to the fermentation product from which fat is removed.

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum is Lactobacillus delbrueckii subsp . bulgaricus ) and Lactobacillus rhamnosus GG are more preferably fermented as a combination of one or more lactic acid bacteria and Streptococcus thermophilus .

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum may be preferably applied with the casein component removed.

According to the present invention, the anti-atopic composition may be used as an anti-atopic cosmetic composition.

In addition, the composition for anti-atopy of the present invention can be applied as a pharmaceutical composition for the treatment and prevention of atopy.

In addition, the anti-atopic composition of the present invention can be applied as a pharmaceutical composition for the treatment and prevention of psoriasis.

In addition, the composition for anti-atopy of the present invention can be applied as a health functional food for anti-atopy.

The fermentation product of colostrum according to the present invention increases the specific active ingredient contained in colostrum through fermentation, and in particular has excellent activity for improving atopic skin, so that it is particularly excellent for atopic skin compared to similar ingredients such as colostrum or milk. It shows improvement effect.

In particular, the fermented product of colostrum according to the present invention is simple and easy to manufacture, and is fermented using microorganisms conventionally used in food production, and thus has no human toxicity and high stability.

Therefore, in the case of preparing cosmetics using the fermented product of colostrum according to the present invention, it is useful as an anti-atopic cosmetic composition for improving atopic skin, unlike the effect expressed in the existing colostrum components.

In particular, according to the present invention, unlike other bacteria, the treatment of skin diseases such as atopic and psoriasis, which is difficult to treat and is a chronic problem when applied to the skin due to fermentation products selectively fermented with certain complex bacteria And has a very useful effect in prevention.

1 is an experimental example for the fermentation product of colostrum according to the present invention, as a result of comparative experiments on the cytotoxic effect of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Examples, by concentration of each sample It is a graph showing the measurement result of 12-hour cell viability through MTT assay.
Figure 2 is an experimental example for the fermentation product of colostrum according to the present invention, the results of AQP3 mRNA expression using a keratinocyte for the colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example (aquaporin experiment) results As a graph showing the results of comparative experiments for measuring the mRNA expression level by concentration of each sample.
3 is an experimental example for the fermentation product of colostrum according to the present invention, a graph showing the PCR results for each sample of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in the Examples.
Figure 4 is an experimental example for the fermentation product of colostrum according to the present invention, for each sample of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example using THP-1 cell to inhibit NO production inhibitory activity As a result of the measurement, it is a graph showing the results of comparative experiments on the inhibitory activity of NO production by concentration of each sample.
Figure 5 is an experimental example for the fermentation product of colostrum according to the present invention, prostaglandin E2 production inhibitory activity using THP-1 cell for each sample of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example As a result of the measurement, it is a graph showing the comparative actual results for the inhibitory activity of PGE2 production by concentration of each sample.
Figure 6 is an experimental example for the fermentation product of colostrum according to the present invention, IL-1β, TNF using THP-1 cells for each sample of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example -α, IL-8 is a graph showing the results of comparative experiments for measuring mRNA expression levels.
7 is an experimental example of the fermentation product of colostrum according to the present invention, as a result of measuring the hyaluronidase inhibitory activity for each sample of colostrum fermentation product and milk, milk fermentation product and colostrum prepared in the Examples, of each sample It is a graph showing the results of comparative experiments on hyaluronidase inhibitory activity by concentration.

Hereinafter, the present invention will be described in more detail as one embodiment as follows.

The present invention relates to a novel use of colostrum fermentation products as a more useful fermentation product obtained from colostrum.

Since the fermentation product obtained by fermentation of colostrum obtained in the present invention has an excellent effect of improving atopic skin, it can be used by replacing and supplementing the existing side effects drugs of chemical synthetic cosmetic compositions or steroid preparations.

According to a preferred embodiment of the present invention, as the fermented product of colostrum, an aerobic fermented product of colostrum fermented under aerobic conditions can be preferably used.

According to a preferred embodiment of the present invention, the fermentation product of colostrum may preferably be aerobic fermentation product from which fat is removed.

According to a preferred embodiment of the present invention, it is preferred that the aerobic fermentation product of colostrum contains any one or more of fermented milk, fermented broth with casein removed, fermented fermented broth, and any one of the fermented milk extract with lactic acid bacteria fermented. Can be used.

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum may be Lactobacillus streptococcus thermophilus colostrum colostrum dissolution filtrate or fermentation extract obtained by filtering the obtained lysate.

According to an embodiment of the present invention, the aerobic fermentation product of colostrum is Lactobacillus delbrueckii subsp. Bulgaricus and Lactobacillus rhamnosus GG ) and Streptococcus thermophilus ( Streptococcus thermophilus ) may be preferably used that is fermented with one or more bacteria selected from.

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum is Lactobacillus delbrueckii subsp. bulgaricus ) and one or more of Lactobacillus rhamnosus GG may be more preferably used as a fermented mixture of lactic acid bacteria and Streptococcus thermophilus .

According to a more preferred embodiment of the present invention, the aerobic fermentation product of colostrum is Lactobacillus delbrueckii subsp . bulgaricus ) and Lactobacillus rhamnosus GG and Streptococcus thermophilus are mixed and fermented with 3 types of complex bacteria.

Further in accordance with a preferred embodiment of the invention, when the fermented colostrum composite bacteria of a specific lactic acid bacterium or Lactobacillus bacteria (Lactobacillus) and Streptococcus bacteria (Streptococcus) applied to the atopic skin of natural cosmetic and medicinal compositions wherein atopic effect Invented a new use that has the effect of preventing and treating atopic dermatitis pruritus such as psoriasis.

According to one embodiment of the present invention, the fermentation product of colostrum may be preferably a milk or colostrum fermentation lysis filtrate or fermentation extract of Lactobacillus streptococcus thermophilus, which has filtered the lysate obtained after fermentation.

According to a preferred embodiment of the present invention, the fermentation product of colostrum is hydrated lactobacillus and S. pneumoniae colostrum colostrum extract (Hydrolyzed Lactobacillus / Streptococcus Thermophilus) / Colostrum Ferment Extract) can be preferably used.

The present invention can be used as a more preferable composition for improving atopic skin of fermented products that are fermented efficiently by using colostrum as a fermentation raw material, for example, by using a synergistic effect of a specific complex bacterium.

According to the present invention, such a colostrum fermentation product of colostrum, in particular, aerobic fermentation product, is a composition for improving atopic skin and exhibits an anti-atopic cosmetic composition that exhibits skin improvement effects, atopic dermatitis pruritus, psoriasis, etc. . This effect can obtain a more excellent synergistic effect by fermenting with a specific complex bacteria selectively applied.

According to a preferred embodiment of the present invention, the fermentation product of colostrum that can be used for the purpose may include any one or more of fermentation broth obtained by fermenting colostrum with the specific complex, additional fermentation broth with casein removed, and sterilized fermentation broth. You can. More preferably, the fermentation broth in which casein is removed is good.

Colostrum used as a fermentation raw material in the present invention can be preferably used milk salted within 3 days after delivery.

The present invention, colostrum used as a fermentation raw material may preferably use bovine colostrum, wherein colostrum is a concept generally including colostrum of livestock animals such as goats, goats, and the like.

According to a preferred embodiment of the present invention, in order to use colostrum, for example, colostrum is rapidly cooled and then pasteurized to prepare a fermentation raw material.

The pasteurization treatment in the present invention can be performed by a conventional pasteurization method.

According to a preferred embodiment of the present invention, colostrum can be preferably used to remove fat. Fat removal can be performed by separation by density difference.

According to a preferred embodiment of the present invention, a step of obtaining a fermentation broth by inoculating and fermenting lactic acid bacteria in the fermentation raw material obtained in the above step may be performed.

According to a preferred embodiment, the lactic acid bacteria fermentation has Lactobacillus Bulgaria kusu (Lactobacillus delbrueckii subsp. Bulgaricus), Lactobacillus ramno suspension support (Lactobacillus rhamnosus GG ) can be preferably used a complex lactic acid bacteria mixed with Streptococcus thermophilus selected from one or more lactic acid bacteria, the selective use of these specific complex bacteria is important for achieving a desirable purpose, for example, to achieve a synergistic effect Element.

According to the invention, for example Lactobacillus delbrueckii subsp . bulgaricus ) produces D-Lactic acid in the fermentation process, exhibits a detoxifying and anti-inflammatory effect, and can express an anti-atopic effect and an effect of improving skin texture.

In addition, Lactobacillus rhamnosus GG ) can be effective in preventing eczema and preventing atopy, and the gram-positive staphylococcus aureus interferes with the growth of keratinocytes and prevents epithelial cell migration and cell reproduction, re-epithelialization ( re-epithelialization).

Therefore, in the present invention, it is preferable to select and use among the two bacteria among Lactobacillus .

In addition, Streptococcus thermophilus may have a desirable effect on skin, such as the formation of a stratum corneum layer structure or an increase in the amount of ceramide necessary for this function.

In addition, according to the present invention, for example, Lactobacillus rhamnosus GG ) and Streptococcus thermophilus produce interleukin-10 as a synergistic effect due to the mixed use, and have excellent anti-inflammatory and anti-atopic effects, and prevention and treatment effects of psoriasis and thymus. Can be represented.

According to the present invention, when the complex bacteria as described above is used, it enhances the anti-inflammatory and anti-atopic effects together with colostrum lactoferrin, and exhibits a synergistic effect in the treatment of atopic skin diseases such as psoriasis and plaque.

For example, as a preferred example, the use of Lactobacillus rhamnosus GG can express the effect of preventing eczema and preventing atopy, and Gram-positive bacteria Staphylococcus aureus interferes with growth in the keratinocytes or , It can help exfoliate cell migration and cell reproduction and express an effect contributing to re-epithelialization.

In addition, Streptococcus thermophilus may have a desirable effect on skin, such as the formation of a stratum corneum layer structure or an increase in the amount of ceramide necessary for this function.

According to the present invention, Lactobacillus rhamnosus as described above GG ) and Streptococcus thermophilus can significantly increase the therapeutic activity of atopy and skin psoriasis through the fermentation of colostrum, and this atopicity is caused by several complex ingredients such as lactoferrin in colostrum. It may have a synergistic effect that further enhances the skin improvement effect.

However, the above various functional effects are assisted by the complex fermentation bacteria used, but are inherently due to the effect of the fermentation product obtained after fermentation of colostrum, and in particular, colostrum is fermented with the specific selective complex as described above. In that case, the effect appears to be a better synergistic effect.

According to the present invention, Lactobacillus delbrueckii subsp . Bulgaricu s and Streptococcus thermophilus , which are used as lactic acid bacteria, for example, provide polyamines to each other of two bacteria to protect themselves from oxidative stress. In the sense that it can be expected more favorable synergistic effect in the case of mixed use.

More preferably, Lactobacillus delbrueckii subsp . bulgaricus , Lactobacillus rhamnosus GG ) and Streptococcus thermophilus ( Streptococcus thermophilus ), when used together with the three types of bacteria may exhibit a more enhanced synergistic effect.

According to a preferred embodiment of the present invention, fermentation using the above-described complex bacteria is inoculated with the above-described complex bacteria in pasteurized colostrum, which is a fermentation raw material, and more preferably, at a temperature of 30-50 ° C. under aerobic conditions. It can be fermented for 120 minutes.

According to a preferred embodiment of the present invention, after the fermentation process, the fermentation product of colostrum can be obtained through a step of centrifuging it to obtain a fermentation broth.

According to a preferred embodiment of the present invention, the fermentation product of colostrum according to the present invention may be one in which casein is removed.

Therefore, according to a preferred embodiment of the present invention, the fermentation product of colostrum by the specific complex as described above may preferably further include the step of obtaining a fermentation extract using an enzyme of the fermentation broth.

According to the present invention, the step of obtaining a fermentation extract using an enzyme of the fermentation broth is preferably performed through a coagulation process for purifying colostrum fermentation broth, for example, which has undergone fermentation.

Therefore, according to a preferred embodiment of the present invention, the fermentation broth may include the step of obtaining a fermentation product of colostrum removed by agglutinating casein using an enzyme.

In this process, it is intended to remove those that may have a negative effect, such as casein contained in the fermentation broth obtained by fermentation of lactic acid bacteria, it is possible to increase the content of the functional component by removing the casein having a high content in the milk protein. Therefore, an enzyme is added to undergo a process of removing components such as casein through a coagulation reaction.

Therefore, according to a preferred embodiment of the present invention, after the enzyme addition, it may further include a purification process including the step of centrifuging and filtering the enzyme additive. After this process, the fermented product of colostrum can be obtained in the form of lactic acid bacteria fermented colostrum extract, which is a more refined fermentation product.

According to a preferred embodiment of the present invention, as an enzyme used for the removal of casein, for example, an enzyme complex for a coagulase role can be used, and specifically, for example, a rennet can be preferably used.

The rennet exemplified herein refers to an enzyme complex in the ruminant's stomach. That is, Rennet is a coagulase containing rennin, which is one of proteolytic enzymes, and is known to be contained with pepsin in the gastric juice of calf. It is known to have the properties of sedimentation, and cheese aged shortly using the cow's gastrointestinal enzyme rennet is known to have a good aroma and smooth mouthfeel.

It can be understood that the enzyme used in the present invention includes all of the enzymes exhibiting properties similar to the aforementioned Rennet.

According to the present invention, when an enzyme represented by such a rennet is used, it can be preferably used to remove the casein by causing agglutination reaction with casein contained in the fermented colostrum obtained by fermentation with the complex bacteria containing the lactic acid bacteria as described above.

According to a preferred embodiment of the present invention, in the enzyme addition process, preferably, the fermentation broth of colostrum obtained after fermentation of the complex bacteria may be agglutinated for 30 to 120 minutes after adding the enzyme at 30 to 40 ° C.

In addition, according to a preferred embodiment of the present invention, in the process of adding the enzyme, the process of agglutinating the components such as casein may be additionally followed by centrifugation and filtration to recover the fermentation broth. By going through this process, it is possible to obtain a fermented product of colostrum in which casein is substantially removed. The fermented product of colostrum thus obtained may be prepared, for example, in the form of lactic acid bacteria fermented colostrum extract from which casein has been removed.

Another embodiment includes a fermented product of colostrum obtained by fermenting colostrum from which fat and casein have been removed as fermentation bacteria under aerobic conditions.

According to a preferred embodiment of the present invention, when the casein is removed before fermentation as described above, and then fermented using a fermentation raw material, the casein removal process as described above may be additionally performed even after fermentation.

Therefore, according to a preferred embodiment of the present invention, the fermented product of colostrum of the present invention can be preferably obtained from the casein removed before, after or before fermentation of colostrum.

In another case, according to a preferred embodiment of the present invention, for example, the fermentation product of colostrum can be prepared as a fermentation product of colostrum containing Glycomacropeptide (GMP), for example, by treatment with rennet or by other methods. .

According to a preferred embodiment of the present invention, the fermentation product containing glycomacropeptide in the fermentation product of colostrum has not been prepared. These glycomacropeptides are known to be produced in the process of cheese making, but they are well soluble in water, so they are removed and discarded along with the byproducts of cheese making.

In general, the glycomacropeptide contains a considerable amount of sugar components, but it has the ability to neutralize the toxins of microorganisms produced by E. coli or Vibrio, has the ability to increase the activity of immunity, helps the growth of bifidobacteria in the intestine, lowers blood pressure and It has functions such as smoothing blood flow by reducing blood clotting. In addition, it prevents tooth decay from adhering to the teeth and is good for dental health.

In addition, the sialic acid component contained in the glycomacropeptide is effective in developing brain function and improving memory as a result of animal experiments.

In addition, it promotes digestive function, appetite control, pancreatic digestive enzyme secretion, and secretion of cholecystokinin in the small intestine, thereby affecting skin beauty. In addition, it can be expected to be effective in improving atopic skin.

Therefore, according to a preferred embodiment of the present invention, the fermented product of colostrum according to the present invention contains a glycomacropeptide and is useful as a health functional food. It is also useful as a pharmaceutical composition for improving the symptoms.

According to a preferred embodiment of the present invention, the aerobic fermentation product of colostrum according to the present invention may contain a glycomacropeptide in 0.001-15% by weight.

According to a preferred embodiment of the present invention, the fermented product of colostrum may be prepared through additional filtration and sterilization.

As such, the lactic acid bacteria fermented colostrum extract obtained according to the present invention may be used by removing bacteria that may be present through filtration twice or several times after preparation.

According to the present invention, the lactic acid bacteria fermented colostrum extract obtained through the above steps is a preferred example of the fermentation product of colostrum, and it was confirmed that it exhibits very useful properties different from any components derived from colostrum.

Therefore, the fermentation product of colostrum according to the present invention is a fermentation broth obtained by fermenting colostrum as a complex bacterium containing a specific lactic acid bacteria, a fermentation broth substantially removed from casein, a fermentation broth treated with fermentation broth, a fermentation product having a fermentation broth purified or colostrum fermented colostrum Any one or more of the extracts may be included. One example of the most preferred type as a fermentation product of colostrum according to the present invention may be purified lactobacillus fermented colostrum extract that does not contain casein by purifying the fermentation broth.

According to a preferred embodiment of the present invention, as described above, by fermenting colostrum with a specific complex bacterium, an excellent effect can be exhibited unlike the prior art. In particular, the specific lactic acid bacterium and the Streptococcus thermophilus bacillus of the present invention are selected and complex When used, it exhibits a synergistic effect, and thus, it is possible to obtain surprising results that can be expected to have an excellent synergistic effect exhibiting superior activity compared to the use of complex bacteria or lactic acid bacteria alone.

As described above, the fermented product of colostrum according to the present invention can obtain a particularly useful effect for improving atopic skin, and additionally, through the purification process, by eliminating the disadvantages caused by casein and the like, while increasing the content of the functional ingredient, Moisturizing and anti-atopic effects showing desirable results, and atopic dermatitis pruritus and treatment and prevention of atopic skin diseases such as psoriasis and thyroid gland can be simultaneously obtained, thereby exhibiting an unexpectedly superior synergistic effect.

On the other hand, the present invention is to prepare and replace natural chemical composition using the fermentation product of acetic acid according to the present invention in order to replace and supplement the existing cosmetic composition, which is a chemical synthetic cosmetic composition, for moisturizing and anti-atopy containing this as an active ingredient And providing a cosmetic composition.

In addition, the fermented product of colostrum according to the present invention, for example, especially colostrum fermented extract, has a surprising effect of showing synergistic effect on the treatment of psoriasis and thymus in addition to the skin moisturizing and atopy treatment effects by fermentation of colostrum.

Accordingly, the present invention includes the provision of a pharmaceutical composition for the treatment and prevention of atopy or psoriasis.

The colostrum fermentation product eliminates the disadvantages caused by casein and the like during the purification process, and at the same time increases the content of the functional ingredient, and has a superior skin moisturizing power than milk fermentation product or colostrum, and atopy such as atopic or psoriasis. It can show the effect on skin improvement.

Psoriasis is a disease of erythematous papules and plaques of various borders and sizes, covered with silver-white scales on the skin, characterized by overgrowth of the epithelium, and chronic inflammatory repetition of exacerbation and improvement. It is a skin disease.

The cause of psoriasis is generally considered to be caused by genetic factors, environmental deterioration or triggers, and immunological factors, but it is not clear. At present, psoriasis is known to cause the proliferation and inflammatory reaction of keratinocytes by immunological factors as an individual's life and environmental factors act as triggers under genetic factors. Deterioration or triggers for psoriasis include skin trauma, infections, cold and dry climates such as winter, dry skin, stress, and drugs, which are associated with skin moisturization.

Psoriasis occurs mainly in symmetry, and occurs in areas that are highly irritated, such as shin, elbow, knee, sacrum, scalp, and neck. In the beginning, psoriasis develops a small red-colored rash on the skin, which gradually grows. And a new rash like a new millet is formed around it, and when it grows, it merges with each other. In the psoriasis area, white scales such as scales appear layered on top of each other, and when the scales are removed, there is a problem of spot bleeding.

Taeseon (苔癬) refers to the condition of thickening of the skin, which is often scratched on the skin, resulting in thickening of the skin and severe wrinkles, which makes it look like an elephant skin. This is called the thyroidization phenomenon, and the rash that appears when thyroidization occurs does not cause blister formation, purulent or skin destruction, but rarely occurs blisters.

In particular, the pores of the pores are small red or gray papules protruding sharply around the pores. Appears well on people with dry skin, and is best seen on the limbs, elbows and shoulders.

According to a preferred embodiment of the present invention, unlike the existing milk or colostrum, the colostrum fermentation product of the present invention is excellent in anti-inflammatory and antibacterial effects, and contains in the colostrum fermentation product while exhibiting a skin moisturizing effect and excellent therapeutic activity of atopy and psoriasis. By remarkably improving the atopic skin environment by various ingredients, it can be suitably used as a cosmetic composition for moisturizing and anti-atopy of natural ingredients or as a pharmaceutical composition for treating and preventing atopy or psoriasis.

Therefore, according to a preferred embodiment of the present invention, a fermentation product of colostrum, more preferably, a cosmetic composition for anti-atopy comprising an aerobic fermentation product of colostrum as an active ingredient.

According to a preferred embodiment of the present invention, a fermentation product of colostrum, and more preferably a pharmaceutical composition for treating and preventing atopy containing aerobic fermentation product of colostrum as an active ingredient.

According to a preferred embodiment of the present invention, a fermentation product of colostrum, more preferably, a pharmaceutical composition for treating and preventing psoriasis containing an aerobic fermentation product of colostrum as an active ingredient.

According to a preferred embodiment of the present invention, a fermented product of colostrum, more preferably, includes a health functional food for anti-atopic that contains an aerobic fermentation product of colostrum as an active ingredient.

According to a preferred embodiment of the present invention, a fermentation product of colostrum, more preferably, a health functional food for psoriasis improvement and prevention, which contains aerobic fermentation product of colostrum as an active ingredient.

According to a preferred embodiment of the present invention, the fermentation product of colostrum in the cosmetic composition or pharmaceutical composition may be contained in an amount of 0.01 to 40% by weight based on the total weight of the composition. If it is outside the lower limit of the above content range, its effect is significantly reduced, and if it is outside the upper limit, it is difficult to manufacture into a cosmetic or pharmaceutical formulation, or it is not desirable from an economical point of view.

According to a preferred embodiment of the present invention, the fermented product of colostrum prepared by the above method may be added in various forms to a cosmetic composition or a pharmaceutical composition as, for example, a fermented colostrum extract, for example, powder, liquid, nanocapsule, It may be added in the form of a complex, and may be added in a liquid form as a preferred example.

According to the present invention, the cosmetic composition containing the fermented product of colostrum can be used as cosmetic formulations such as lotion (skin lotion), nutrient lotion, nourishing cream, massage cream, nutrient essence, cleanser, mask pack, etc. It is not intended to be used in other hair preparations or skin preparations for various functional effects.

In the case of the cosmetic composition of the colostrum fermentation product according to the present invention, the raw material is colostrum, and the fermentation bacteria used to ferment it is a natural microorganism that has been conventionally used for food production, and thus has high safety for the extract itself. It has a superior effect on the treatment and prevention of psoriasis compared to milk, fermented milk, colostrum, and the like.

Particularly, by fermenting colostrum, but using specific complex bacteria, it exhibits a remarkable atopic skin improvement effect that is more unpredictable due to the synergistic effect of selective complex fermentation compared to the use of complex bacteria of other lactic acid bacteria and other bacteria.

As described above, when the colostrum fermentation product according to the present invention is applied as a cosmetic composition for anti-atopy applied as a composition for an atopic skin improvement composition, or used as a cosmetic on the skin or as a pharmaceutical composition for the treatment and prevention of atopic or psoriasis Therefore, for example, when used as a cream or skin ointment, synergistic effects such as atopic skin improvement and atopic skin disease treatment can be expected, which is expected to be very useful as a new concept cosmetic composition or pharmaceutical composition.

The cosmetic composition or pharmaceutical composition containing the colostrum fermentation product of the present invention as an active ingredient can be applied by applying it to the skin several times at specifically 1-500 mg / ml.

According to a preferred embodiment of the present invention, more preferably 10-400mg / ml can be applied.

According to a preferred embodiment of the present invention, the skin using the cosmetic composition for anti-atopy containing the colostrum fermentation product according to the present invention as an active ingredient, or the skin for the treatment and prevention of atopy or psoriasis containing the colostrum fermentation product as the active ingredient The ointment can be prepared and applied in various contents such as 50 mg / ml, 100 mg / ml, 200 mg / ml, 400 mg / ml within the above content range.

In addition, the health food or oral pharmaceutical composition according to the present invention may be administered orally, for example, 50 mg, 100 mg, 200 mg, 400 mg, 500 mg or 1,000 mg once a day as an oral preparation.

According to a preferred embodiment of the present invention, if the colostrum fermentation product exceeds the lower limit of the above content range, its effect is significantly reduced, and if it exceeds the upper limit, it is difficult to prepare as a composition or formulation for skin application, or compared to the added amount It is not desirable from an economic point of view because the expected effect does not improve significantly.

Hereinafter, the present invention will be described in detail by examples, but the present invention is not limited by the examples.

Example 1

To prepare a fermented colostrum extract, which is a fermentation product from colostrum, a fermented colostrum extract was prepared through the following process.

Step 1: Preparation of raw materials

After rapidly cooling the colostrum of freshly squeezed cows into a constant temperature water tank, the temperature of colostrum is sterilized for 30 minutes from 63 ° C, and then the temperature is lowered to 35 ° C to prepare fermented raw materials.

Step 2: Preparation of lactic acid bacteria fermentation broth

Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842), Streptococcus thermophilus ( ATCC 19258), Lactobacillus rhamnosus GG , ATCC 53103) were inoculated into the first step fermentation material and inoculated to the aerobic fermentation material. Under conditions, fermentation (fermentation liquid) is obtained by fermentation at a temperature of 35 ° C for 60 minutes.

Step 3: Preparation of fermented colostrum extract

The fermentation broth obtained in the second step is centrifuged at 2000 rpm for 20 minutes to remove suspended matter, and the supernatant excluding the precipitate is recovered to obtain the fermented colostrum as the fermentation product of colostrum.

The fermented colostrum was adjusted to 33 ° C. and Rennet was added to aggregate for 60 minutes, and then the fermented colostrum was recovered. The fermented colostrum obtained above is centrifuged at 13000 rpm for 60 minutes to obtain the supernatant as a fermented colostrum colostrum extract.

After the second supernatant was filtered with 0.4 μm and 0.2 μm filter paper, a fermented colostrum extract was prepared as a final fermented product of lactic acid bacteria.

Examples 2-6

In Example 1, the microorganisms or the complex bacteria inoculated in the fermentation raw material of the first step were adjusted as shown in Table 1 below to prepare colostrum fermentation products in the same manner as in Example 1.

division Strain used Example 2 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842) Example 3 Lactobacillus rhamnosus GG , ATCC 53103) Example 4 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842)
+ Lactobacillus rhamnosus GG , ATCC 53103) Example 5 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842)
+ Streptococcus thermophilus (ATCC 19258) Example 6 Lactobacillus rhamnosus GG , ATCC 53103)
+ Streptococcus thermophilus ( ATCC 19258)

Example 7

The fermentation product of colostrum was prepared in the same manner as in Example 1, but using Streptococcus thermophilus (ATCC 19258) alone as a bacterium used for fermentation.

Example 8

In the method of Example 2, Lactobacillus bulgaricus ( Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842) was carried out in the same manner as in Example 2, except that Lactobacillus acidophilus (ATCC 4356) was used, to prepare a fermented colostrum extract.

Example 9

Lactobacillus delbrueckii subsp . except for using the bulgaricus, ATCC 11842), Streptococcus Thermo filler's (Streptococcus thermophilus, ATCC 19258) instead of Lactobacillus ash FIG pillar's (Lactobacillus acidophilus, ATCC 4356 on), Streptococcus Thermo filler's (Streptococcus thermophilus, ATCC 19258) And it was carried out in the same manner as in the method of the above example, to prepare a fermented colostrum extract.

Comparative Example 1-9

Fermentation was performed in the same manner as in Example 1-9, but fermentation was performed under anaerobic conditions, not aerobic conditions.

Hyaluronidase enzyme activity, IL-8 production inhibitory effect, NO production inhibitory effect, PGE2 production inhibitory effect, moisturizing activity measurement (aquaporins), moisturizing ability measurement evaluation through moisture weight change, Corneometer moisture amount to confirm the above effects Evaluation, TEWL change experiment was conducted. The following experimental example is a result value showing excellent efficacy.

Experimental Example 1: Cytotoxicity experiment

Cell viability was measured for each sample through MTT assay.

MTT assa y is a test method that is frequently used for cell proliferation or toxicity by measuring the number of living cells, and living cells are water-soluble and yellow salts of MTT in mitochondria, a blue formazan derivative that is water-insoluble by succinic dehydrogenase. It is to use the principle of reduction.

First, 6-well plates were inoculated with HaCaT cells at a density of 1x10 ^ 5 cells / well and cultured at 37 ° C for one day. After that, each sample (milk, milk fermentation, colostrum, colostrum fermentation) was treated in each well by concentration (1,10,20 ug / ml). After incubation at 37 ° C for 12 hours, the MTT solution was treated and activated for 1 hour, and then measured at 450 nm with an ELISA reader. Control was conducted with H2O2, and the cell viability was compared to the control group.

The results of the experiment are shown in Table 2 and FIG. 1, and it was confirmed that the fermented colostrum, a colostrum fermentation product, exhibits superior survival rate compared to other comparison groups.

12H   control 100  0.2    milk  1ug / ml 90  0.05  10ug / ml 118  0.1  20ug / ml 107  0.04    Milk fermentation  1ug / ml 100  0.06  10ug / ml 105  0.18  20ug / ml 94  0.1    beestings  1ug / ml 81  0.1  10ug / ml 108  0.11  20ug / ml 106  0.07    Colostrum fermentation  1ug / ml 122  0.18  10ug / ml 126  0.14  20ug / ml 125  0.26

Experimental Example 2: Confirmation of AQP3 mRNA expression using Keratinocyte

There are 13 aquaporins (AQP0-AQ P12) in mammals. When identified as skin-related cells such as human keratinocytes, melanocytes, and skin fibroblasts, a total of six AQPs 1, 3, 5, 7, 9, and 10 can be found. Among them, AQP3 is found in Epidermis and serves to permeate both water and glycerol. In conclusion, AQP3 deletion shows a normal skin structure, but has the effect of reducing moisture in the stratum corneum and reducing the permeability of water and glycerol epidermis. Accordingly, the moisturizing effect was confirmed using the expression level of AQP3 mRNA.

As an experimental method, first, dispense keratino cyte to 4x10 ^ 5 cells / well in a 6-well plate, incubate for 24 hours, and then each sample (milk, milk fermentation, colostrum, colostrum fermentation) by concentration (0, 1, 10, 100 ug) / ml). After 3 hours, cells were collected and RNA was extracted to synthesize cDNA. Real-time PCR was performed with the produced cDNA to measure the expression level of mRNA, and it showed an increase rate compared to the no-addition group.

The results are shown in Table 3 and FIG. 2, and FIG. 3 shows PCR results for each sample.

As a result of the test, no significant moisturizing effect was obtained in milk and colostrum. However, in milk fermentation and colostrum fermentation, it was confirmed that mRNA expression was increased in a concentration-dependent manner. Particularly, in 100 ug / ml of colostrum fermentation group, the mRNA expression was increased by 8 times or more compared to the non-addition group, and it was confirmed that the moisturizing effect was very excellent.

Experimental Example 3: Nitric oxide (NO) production inhibitory activity measurement

Lipopolysaccharide (LPS), which is mainly used as an inflammatory model, activates macrophages. Activated macrophages are inflammation-inducing cytokines TNF-α. IL-1β, IL-12, IFNγ increases the amount of expression. Inflammation-induced cytokines produce Nitric oxide (NO). Therefore, the anti-inflammatory effect was evaluated by reducing the amount of NO expression.

As an experimental method, 1 x 10 ^ 6 of THP-1 cells were added to each well of a 12 well plate, and LPS was mixed to a prepared sample at a concentration of 1 µg / ml and treated simultaneously with the cells to be treated at 37 ° C and 5% CO2 incubator 24 Incubated for an additional hour. After incubation, 100 μl of cell supernatant and 100 μl of Griess reagent were mixed and reacted in a 96 well plate for 10 minutes, and absorbance was measured at 540 nm. The amount of NO produced was measured in the form of NO2- present in the cell culture using Griess reagent, and sodium nitrite (NaNO2) was used as a standard.

The LPS-only treatment group induced the production of NO, and the NO production inhibitory activity for each sample was measured.

The results are indicated in FIG. 4.

Experimental Example 4: Prostaglandin (Prostaglandin E2) production inhibitory effect confirmed

We investigated the inhibitory effect of prostaglandin E2 (PGE2) production, which is known to play an important role in causing inflammation. Cyclooxygenase (COX) is an enzyme that converts arachidonic acid to prostaglandin and is classified into COX-1 and COX-2. COX-1 acts on normal biological functions, such as the formation of platelets in the body, protection of the stomach wall, and maintenance of renal function, and COX-2 forms PGE2, an inflammatory mediator. PGE2 is known to be deeply involved in cancer development, including inflammatory reactions, immune responses, and angiogenesis.

As an experimental method, 1 x 106 cells were dispensed for each well of 12 well plates of THP-1, and LPS was mixed with the prepared sample at a concentration of 1 µg / ml and treated simultaneously with the cells at 37 ° C and 5% CO2 incubator. The cells were further cultured for 24 hours. The amount of PGE2 produced was calculated and expressed by the following equation 1 using the PGE2 ELISA kit. The control group was an experimental group treated with LPS alone. The inhibition rate of PGE2 production was calculated by the following equation, and the results are shown in FIG. 5.

[Equation 1]

(In the above formula, S is the amount of PGE2 in the sample treatment, C is the amount of PGE2 in the experimental group treated with LPS alone.)

Significance was tested through test (P <0.01).

Experimental Example 5: Confirmation of inflammatory factor mRNA expression using monocyte

Increased concentrations of IL-1β, TNF-α, and IL-8 as essential factors involved in neutrophil migration in acute inflammatory reactions are used as indicators of inflammatory responses. Accordingly, the inflammatory response was confirmed by confirming a change in mRNA expression levels of IL-1β, TNF-α, and IL-8.

As an experimental method, first disperse monocyte THP-1 cells into 12 well plates to 1x10 ^ 6 cells / well, and incubate for 24 hours, and then each sample (milk, milk fermentation, colostrum, colostrum fermentation) by concentration (0, 1, 10 , 100%). After 24 hours, cells were collected and RNA was extracted to synthesize cDNA. Real-time PCR was performed with the produced cDNA to measure the expression level of mRNA, and it showed an increase rate compared to the no-addition group.

      The resulting values are indicated in FIG. 6.

Experimental Example 6: Measurement of the inhibitory effect of inflammatory enzymes (hyaluronidase) activity

Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid and causes inflammation. In addition, hyaluronic acid is a component that increases the moisturizing power, and when decomposed, it causes a decrease in moisturizing power. The measurement of hyaluronidase inhibitory activity is reacted with p-dimethylamino benzaldehyde (DMAB) added as a colorant after N-acetylglucosamine formed from sodium-hyaluronic acid (HA) added to the substrate is transformed into a glucoxazoline derivative. By measuring the degree of decrease in absorbance of the colored one, the enzyme inhibitory effect was measured.

As an experimental method, 0.05 ml of hyaluronidase (7,900 unit / mL) dissolved in 0.1 M acetate buffer (pH 3.5) and 0.1 ml of sample solution (milk, milk fermented, colostrum, colostrum fermented) were mixed for 20 minutes at 37 ° C. After the reaction, 0.1 ml of 12.5 mM calcium chloride (CaCl2) was added, and the mixture was reacted again for 20 minutes. As a control group, 0.05 ml of hyaluronidase (7,900 unit / mL) and 0.1 ml of distilled water were mixed instead of the extract of Sambaekcho, and hyaluronic acid (HA; 12 mg / mL) dissolved in 0.1M acetate buffer (pH 3.5) was added as a substrate. The mixture was reacted for another 40 minutes, and 0.1 ml of 0.4N potassium tetraborate and 0.4N NaOH solution were added to the 0.1 ml reaction mixture, heated in a water bath for 3 minutes, and completely cooled.

To the cooled reactant, 3 mL of a colorant p-dimethylamino benzaldehyde (DMAB) reagent (4 g of p-dimethylamino benzaldehyde dissolved in 350 ml of 100% acetic acid and 50 ml of 10 N hydrochloric acid) was added and reacted at 37 ° C for 20 minutes. The absorbance was then measured at 585 nm to calculate the inhibitory activity. Hyaluronidase inhibitory activity was calculated by the following equation, and the significance of the experiment was verified by Student's T test.

[Equation 2]

(In the above formula, S is the absorbance of the sample and the enzyme-containing reaction solution, C is the absorbance of the sample-free solution.)

The resulting values are indicated in FIG. 7.

Claims (16)

An atopic composition containing colostrum fermentation products as an active ingredient.
The composition according to claim 1, wherein the fermentation product of colostrum is an aerobic fermentation product of colostrum fermented under aerobic conditions.
The method according to claim 1, wherein the fermentation product of colostrum is an atopic composition, characterized in that the fat is aerobic fermentation product is removed.
The method according to claim 2, The aerobic fermentation product of colostrum is Lactobacillus delbrueckii subsp. Bulgaricus and Lactobacillus rhamnosus GG ) and Streptococcus thermophilus ( Streptococcus thermophilus ) composition for anti-atopy, characterized in that fermented with at least one selected from bacteria.
The method according to claim 2, wherein the aerobic fermentation product of colostrum is a fermentation broth, a casein-removed fermentation broth, a sterilized fermentation broth, and any one or more of the fermentation broth of the lactic acid bacteria fermented colostrum extract is purified Dragon composition.
The composition according to claim 2, wherein the aerobic fermentation product of colostrum is Lactobacillus streptococcus thermophilus colostrum colostrum dissolution filtrate or fermentation extract obtained by filtering the obtained lysate after fermentation.
The method according to claim 2, aerobic fermentation product of colostrum is not Lactobacillus Riku's (Lactobacillus delbrueckii subsp. Bulgaricus) and Lactobacillus ramno suspension support (Lactobacillus rhamnosus GG ) and Streptococcus thermophilus ( Streptococcus thermophilus ) is a composition for anti-atopy, characterized in that it is fermented as a complex.
The composition according to claim 2, wherein the aerobic fermentation product of colostrum has the casein component removed.
The composition according to claim 2, wherein the aerobic fermentation product of colostrum contains glycomacropeptide (GMP).
The composition according to claim 8, wherein the aerobic fermentation product of colostrum contains 0.001-15% by weight of glycomacropeptide (GMP).
Claim 1 to claim 10, wherein the cosmetic composition for anti-atopy containing the composition for anti-atopy according to any one of claims as an active ingredient.
Claim 1 to claim 10, wherein the pharmaceutical composition for the treatment and prevention of atopy containing an anti-atopy composition according to any one of claims as an active ingredient.
A pharmaceutical composition for the treatment and prevention of psoriasis containing as an active ingredient an aerobic fermentation product of colostrum according to any one of claims 2 to 10.
A skin ointment agent for the treatment of atopic skin diseases, which contains the aerobic fermentation product of colostrum according to any one of claims 2 to 10 as an active ingredient.
A health functional food for improving and preventing atopy containing as an active ingredient an aerobic fermentation product of colostrum according to any one of claims 2 to 10.
Claims 2 to 10, psoriasis improvement and prevention health functional foods containing the aerobic fermentation product of colostrum according to any one of the claims as an active ingredient.

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