KR20200033437A - Double microcapsule comprising complex of ulmus pumila l. pectic polysaccharide and whey protein concentrates, and preparation method thereof - Google Patents

Abstract

The present invention relates to a double microcapsule which has a core material containing gallic acid and is obtained by coating the core material with a first coating material and a second coating material sequentially. The present invention also relates to a manufacturing method thereof. The double microcapsule shows improved digestion stability.

Description

DOUBLE MICROCAPSULE COMPRISING COMPLEX OF ULMUS PUMILA L. PECTIC POLYSACCHARIDE AND WHEY PROTEIN CONCENTRATES, AND PREPARATION METHOD THEREOF}

The present invention relates to a core material containing gallic acid and a double microcapsule sequentially coated with the core material and a primary coating material and a secondary coating material and a method for manufacturing the same.

Gallic acid, natural pigments, vitamins, etc. have a great influence on the productability and sensory properties of the applied food by changing properties due to environmental factors during processing or storage for utilization. As described above, active ingredients sensitive to the external environment are mainly denatured by interactions with ingredients of food itself, including pH, oxygen, moisture, heat and light, and metal ions.

In particular, due to the instability of the active ingredient against the external environment, even when ingesting food containing it, there is a disadvantage that the bioavailability is low because the amount of the active ingredient actually absorbed into the body is small.

Accordingly, attempts have been made to increase the stability of the active ingredient, but since the emulsifier used in the process of increasing the stability is mostly a synthetic material, there is a problem in that cytotoxicity and environmental problems are additionally generated.

Accordingly, research for the use of natural materials having no toxicity while increasing the stability of the active ingredient is continuously required.

Korean Registered Patent 10-185292250

An object of the present invention is to provide a double microcapsule, the core material comprising gallic acid and the core material sequentially coated with a primary coating material and a secondary coating material.

Another object of the present invention is to provide a method for manufacturing the double microcapsules.

One aspect of the present invention for achieving the above object is to provide a double microcapsule, the core material containing gallic acid and the core material sequentially coated with a primary coating material and a secondary coating material.

In the present invention, "gallic acid (gallic acid)" is distributed freely in the roots, stems, leaves, fruits, etc. of various plants including nuts, teas, inhibits the growth of microorganisms or viruses, whitening effect, antioxidant, anti-cancer And anti-diabetic effect. However, there is a disadvantage that it is difficult to maintain effective properties until it is easily oxidized and applied to the body when it comes into contact with the external environment. In the present invention, as described above, the core material containing gallic acid, which is difficult to preserve, was sequentially coated with the primary coating material and the secondary coating material to improve the preservability, and the gallic acid is considered to be one of the materials vulnerable to the external environment. In addition to gallic acid, the invention can be applied to various active ingredients that are easily denatured because they are vulnerable to external environments such as chlorophyll and vitamins.

Encapsulation technology can significantly improve the functionality or physiological function of the core material by emitting it at a constant rate under specific environmental conditions, such as light, oxygen, and moisture, which can affect the core material. It is a technology to protect from the external environment. Encapsulation technology is used in the food industry for the purpose of maintaining intrinsic functionality from an incomplete external environment, preventing oxidation and preservation, blocking off-flavor, solidifying liquid foods, pigments, antioxidants, acids, enzymes, microorganisms, fragrance components, vitamins , Minerals, fats and oils.

In the present invention, the "double microcapsule" is to ensure that the core material is protected with a double coating material in a microcapsule, which is a fine-sized capsule having a size of 1000 μm or less. These double microcapsules can be prepared in several ways, one of which is spray drying (spray drying). The spray-drying method is a method of obtaining double microcapsules by rapidly drying by spraying fine droplets of a solvent in which a substance which is a material of a capsule is dissolved in hot air, and can obtain many double microcapsules in a short time, and emulsification. A double microcapsule can be prepared by spray drying the same suspension. The method of manufacturing the double microcapsules is not limited to the spray drying method, and a person skilled in the art may prepare the double microcapsules using an appropriate method as necessary.

In the present invention, "core material (core material)" refers to a solution, emulsion, oil, powder or the like dissolved by mixing gallic acid in the solvent or the acid itself, and is not limited to a specific form. Any substance containing gallic acid as a main component can be a central substance of the present invention. Furthermore, various active ingredients that are easily denatured because they are vulnerable to external environments such as chlorophyll and vitamins can also be applied to the present invention as a central substance.

In the present invention, "wall material" refers to a material covered with a thin layer to protect gallic acid or a central material containing gallic acid. The coating material is a material that can protect the active ingredient such as gallic acid, which is fairly unstable in the external environment, to maintain stability, and is protected from changes to other chemicals, derivatives, etc. by enzymes, acids, oxygen in the air, light, heat, etc. Can be used. In addition, the coating material may be divided into a primary coating material, a secondary coating material, and the like, and in the present invention, a double microcapsule including a primary coating material and a secondary coating material is formed, and more specifically, the coating. The substance was made to contain the root skin polysaccharide.

In the present invention, "yugeunpi" includes both the bark and the bark of the root of a tree belonging to the elm family (Ulmaceae), and is also called milky white, epithelial, breast milk, and yupi. The root can be derived from various elm trees, but is not particularly limited thereto, specifically, a bissul tree ( Ulmus pumila ), Ulmus parvifolia ), elm ( Ulmus macrocarpa ), antler ( Ulmus) laciniata), elm (Ulmus per davidiana ) can be the bark of various elm trunks and roots. In addition, yugeunpi has been used as a herbal medicine, has excellent antibacterial and bactericidal properties, is helpful in treating rhinitis and sinusitis, and is known to show an effect on inflammation. In the present invention, it was confirmed that the polysaccharide was extracted from the above root oil and the extracted root oil polysaccharide could be used as a secondary coating material of the double microcapsule by forming a complex with whey protein.

Furthermore, by using the natural root oil polysaccharide as a coating material as described above, it is possible to solve the cytotoxicity and environmental problems of the surfactant or synthetic material used as an existing emulsifier, and as a coating material of natural materials, as a cosmetic material as well as a food material. Also applicable.

Specifically, the primary coating material may include medium chain triglyceride (MCT) oil and polyglycerol polyricinoleate (PGPR).

In addition, specifically, the secondary coating material may be one containing the root muscle polysaccharide.

In an embodiment of the present invention, a primary coating material containing medium chain triglyceride (MCT) oil and polyglycerol polyricinoleate (PGPR) is mixed with gallic acid as a central material to form W / O. A primary emulsion was prepared, and a double microcapsule was prepared by mixing a secondary coating material made of a complex formed by mixing whey protein extracted with the primary emulsion with whey protein to prepare a secondary emulsion of W / O / W type. Was prepared.

In particular, in the secondary coating material, the double microcapsules containing the root muscle polysaccharides have high encapsulation efficiency (Table 3), and the storage stability is excellent (Table 4 and FIG. 5). , It was confirmed that it shows an improved digestive stability (Fig. 6).

Specifically, the secondary coating material of the present invention double microcapsules may include the root muscle polysaccharide containing 1.0% to 2.0% by weight.

In addition, specifically, the secondary coating material may include a complex of lactose polysaccharide and whey protein, and more specifically, a complex composed of a ratio of 1: 15 to 1: 30 based on weight percent of lactose polysaccharide and whey protein. It may be included.

In one embodiment of the present invention, a double microcapsule containing a secondary coating material containing 1.0% by weight to 2.0% by weight of the root muscle polysaccharide was prepared, and the root muscle polysaccharide and whey protein were 1: 15 to 1: 30 based on weight%. A double microcapsule was prepared comprising a secondary coating material comprising a composite composed of proportions.

In the case of the double microcapsule containing the above root carcass polysaccharide in the secondary coating material, it showed superior encapsulation efficiency compared to the double microcapsules not containing the root carcass polysaccharide, and the encapsulation efficiency was significant as the content of the contained root carcass polysaccharide increased. It was confirmed that the increase (Table 3). The encapsulation efficiency refers to the degree to which the coating material stably protects the core material, and in the present invention, it was confirmed that the core material protection effect of the secondary coating material containing the root muscle polysaccharide is excellent.

In addition, in the exemplary embodiment of the present invention, in the case of a double microcapsule containing the root carcass polysaccharide in the secondary coating material, the moisture content is significantly lower than the double microcapsule that does not contain the root carcass polysaccharide, thereby minimizing the deterioration caused by moisture, thereby saving the core material It was confirmed that the stability was increased (Table 4), and further, it was confirmed that the storage stability of the double microcapsules containing the root cartilage polysaccharide in the secondary coating material was remarkably excellent at all conditions of 4 ° C, 20 ° C and 40 ° C (FIG. 5).

Furthermore, in the embodiment of the present invention, in the case of the double microcapsules containing the root carcass polysaccharide in the secondary coating material, the digestion stability is improved by suppressing the release of the central substance in the acidic gastric juice compared to the double microcapsules not containing the root carcass polysaccharide. It was confirmed that the bioavailability in the small intestine was increased (FIG. 6).

Another aspect of the present invention a) preparing a primary emulsion (emulsion) by mixing a primary material and a primary material containing gallic acid; And b) preparing a secondary emulsion by mixing the primary emulsion and the secondary coating material.

Specifically, the primary coating material may be a mixture of medium chain triglyceride (MCT) oil and polyglycerol polyricinoleate (PGPR).

In addition, specifically, the secondary coating material may be one containing the root muscle polysaccharide.

In addition, specifically, the secondary coating material of the present invention double microcapsules may include a root oil polysaccharide containing 1.0% to 2.0% by weight.

In addition, specifically, the secondary coating material may include a complex of lactose polysaccharide and whey protein, and more specifically, a complex composed of a ratio of 1: 15 to 1: 30 based on weight percent of lactose polysaccharide and whey protein. It may be included.

In one embodiment of the present invention, the matters confirmed for the encapsulation efficiency, storage stability and digestion stability of the double microcapsules prepared according to the above conditions are as described above. Due to the excellent stability as described above, the double microcapsules of the present invention can be widely used in the food industry, cosmetics industry, such as various foods and food additives.

The double microcapsules of the present invention provide stability to gallic acid, which is unstable to the external environment, so that it can be maintained in a stable state for a long period of time despite changes in the surrounding environment. In addition, even in the strong acid environment of the stomach, the stability of gallic acid is maintained high, thereby lowering the amount of release from gastric juice, thereby improving digestion stability.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 shows the results of (A) Comparative Example, (B) Example 1, (C) Example 2 and (D) Example 3 W / O / W emulsion formation measured by an optical microscope.
Figure 2 shows the results of measuring the zeta potential of Comparative Examples and Examples 1 to 3.
3 shows a scanning electron microscope (SEM) photograph of Comparative Examples and Examples 1 to 3.
Figure 4 is (A) gallic acid, (B) whey protein, (C) root muscle polysaccharide, (D) Comparative Example, (E) Example 1, (F) Example 2 and (G) Example 3 FT- of each IR results are shown.
Figure 5 shows the results of observing the storage stability of Comparative Examples and Examples 1 to 3.
Figure 6 shows the results of measuring the release amount of gallic acid under the composition of artificial gastric juice of Comparative Examples and Examples 1 to 3.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, the present invention is not limited by the following examples.

Manufacturing example  One. Yoo Keun Pyo  Extraction of polysaccharides

The dried root root root skin was crushed 60 g and mixed with distilled water (1: 20, w / v). The cells were incubated at 80 ° C. for 24 hours in a shaking water bath (BS-11, Jeio tech Co., Ltd., Daejeon, Korea). After cooling to room temperature, the suspension was centrifuged at 3500 rpm for 10 minutes (Combi 408, Hanil Biomed, Gwangju, Korea). Ethanol (95%) was added to the supernatant (3: 1, v / v) to precipitate the root muscle polysaccharide, followed by Whatman No. It was filtered through 1 paper (GE Healthcare, Amersham, UK). The solid obtained after filtration was freeze-dried to obtain a polysaccharide (UPC) extracted from the root oil.

Manufacturing example  2. Yoo Keun Pyo  Polysaccharides and Whey protein  Composite production

1.0, 1.5, and 2 g of the root skin polysaccharides obtained in Preparation Example 1 were respectively dispersed in distilled water to prepare 100 g root skin polysaccharide solution. In addition, whey protein was dissolved in distilled water to prepare a 100 g whey protein solution.

The solutions were each gently stirred for 30 minutes with a magnetic stirrer, and the pH was adjusted to 6.3 through a phosphate buffer. The solutions were mixed and stirred overnight at room temperature to fully hydrate. Thereafter, 10 g of maltodextrin was mixed and powdered using a spray dryer to increase the dry substance of the resulting solution, thereby obtaining a complex (WPC-UDP) of milk root polysaccharide and whey protein. The complex of the root muscle polysaccharide and whey protein was used as a secondary coating material.

Manufacturing example  3. Double fine capsule  Produce

W / O / W emulsions were prepared in a two step procedure.

3-1. W / O type primary emulsion  Produce

The primary coating material was prepared by stirring 5 wt% of medium chain triglyceride (MCT) oil and 76 wt% of polyglycerol polyricinoleate (PGPR) until it became transparent at 350 rpm. Subsequently, gallic acid (Sigma-Aldrich Chemical Co. (St. Louis, MO, USA)) was added slowly until 19 w / w% as a central material, and stirred for a sufficient time to form a microemulsion. A primary emulsion of was prepared. MCT oil and PGPR were mixed and homogenized after mixing at different ratios. Specifically, in this example, the ratio of MCT oil: PGPR: gallic acid in the primary emulsion was applied at 19% by weight: 5% by weight: 76% by weight.

3-2. W / O / W type secondary emulsion  Produce

Step 2 was performed at 10 ° C. A DIAX 600 homogenizer (Heidolph, Kelheim, Germany) was used, and gradually added the secondary coating material (complex of milky skin polysaccharide and whey protein) obtained in Preparation Example 2 to the primary emulsion of W / O type 9400 Homogenize for 5 minutes at rpm. It was then further homogenized for 2 minutes at 12,000 rpm. In this example, the multiple emulsions prepared through the above process were applied in a ratio of 75% by weight of the primary W / O emulsion and 75% by weight of the secondary coating material.

Components of Comparative Examples and Examples according to the weight ratio of the root root polysaccharides are shown in Table 1 below. Specifically, Example 1 is a complex of 1.0% by weight of dermal polysaccharide and whey protein, Example 2 is a complex of 1.5% by weight of dermal polysaccharide and whey protein, and Example 3 is a complex of 2.0% by weight of dermal polysaccharide and whey protein. Is to be included.

Multiple emulsions
(multiple emulsion) W / O / W type secondary emulsion W / O type primary emulsion Secondary coating material Inner phase micro emulsion Outer phase Central substance Primary coating material Secondary coating material Gallic acid
(weight%) PGPR
(weight%) MCT oil
(weight%) Whey protein
(weight%) Root muscle polysaccharide
(weight%) Maltodextrin
(weight%) Distilled water Comparative Example (WPC) 19 5 76 30 - 10 Up to 100 Example 1
(WPC-UDP 1.0) 19 5 76 30 1.0 10 Example 2 (WPC-UDP 1.5) 19 5 76 30 1.5 10 Example 3
(WPC-UDP 2.0) 19 5 76 30 2.0 10 Sum (% by weight) 100 100

The formation of the W / O / W emulsions of Comparative Examples and Examples 1 to 3 prepared as described above was measured through an optical microscope, and it was confirmed that all samples exhibited a round shape and a relatively smooth surface (FIG. 1).

3-3. Spray drying  by Of double microcapsules  Produce

Comparative Examples and Examples 1 to 3 obtained in Preparation Example 3-2 were spray-dried to prepare double microcapsules.

Specifically, the conditions of the spray dryer is inlet air temperature 140 ± 5 ℃, exhaust air temperature 85 ± 5 ℃ (spray dryer SD-1000, Eyela, Tokyo, Japan), rotary atomizer 10 × 10 kPa, blower speed 0.60 m ³ / min and the pump rate was 1.0 mL / min, and the dry powder obtained at this time was collected and stored in an airtight container at -20 ° C until further analysis.

Experimental example  One. Of double microcapsules  Evaluation of physicochemical properties

1-1. Zeta potential (Zeta-potential) measurement

The zeta potential was measured to confirm the particle behavior and interaction of the double microcapsules of Comparative Examples and Examples 1 to 3 in which the type and / or content of the secondary coating material were differently prepared. Specifically, 0.3 g of the double microcapsules was diluted in 30 mL of distilled water, and the diluted double microcapsules were injected into a flow cell to form an ELSZ-1000 type zeta-potential & particle size analyzer (Phota Otsuka Electronics, Osaka, Japan). The zeta potential was measured.

The zeta potential values of Comparative Examples and Examples 1 to 3 were -37 mV, -37 mV, -38 mV, and -36 mV, respectively, and did not show a significant difference from each other (FIG. 2). In general, when the zeta potential is higher than ± 30 mV, the emulsion is considered to be stable due to the electrostatic repulsion, so the results indicate that both the comparative examples and the double microcapsules of Examples 1 to 3 are electrostatically stable.

1-2. Of double microcapsules  Particle surface observation

The surface of the particles of the double microcapsules of Comparative Examples and Examples 1 to 3, in which the type and / or content of the secondary coating material was differently observed, was observed with a scanning electron microscopy.

Specifically, the double microcapsules of Comparative Examples and Examples 1 to 3 were sprinkled on double-sided carbon tape and coated with platinum powder. Morphological characteristics of the particles were photographed at a scanning magnification of 5000 by a scanning electron microscope (S-4700, Hitachi CO, Tokyo, Japan) at an acceleration voltage of 10 kV (FIG. 3).

As shown in FIG. 3, in the case of the double microcapsules of the comparative example not containing the root carcass polysaccharide, contraction and surface depression were caused by denaturation of the protein due to high temperature in the spray drying step. On the other hand, in the case of Examples 1 to 3 containing the root sheath polysaccharide in the secondary coating material, the surface was smooth and no crack was observed.

The above results indicate that a double microcapsule having a stabilized particle surface can be prepared by including the root skin polysaccharide in the secondary coating material.

1-3. Of double microcapsules  Particle size measurement

The particle size of the double microcapsules of Comparative Examples and Examples 1 to 3, in which the type and / or content of the secondary coating material was prepared differently, was measured by Malvern Mastersizer 2000 (Malvern Instruments Ltd., Worcestershire, UK).

Specifically, a sample suspension was prepared by mixing the double microcapsules of Comparative Examples and Examples 1 to 3 in 10 mL deionized water at a ratio of 1: 400 (w / v), and particle distribution was monitored during three consecutive reads ( Table 2).

division D _[4,3] (μm) ²⁾ D ₅₀ (μm) ³⁾ D ₉₀ (μm) ⁴⁾ Span Comparative example 2.33 ± 0.02 ^d 2.79 ± 0.03 ^c 9.35 ± 0.37 ^bc 2.93 ± 0.09 ^bc Example 1 2.45 ± 0.01 ^c 3.01 ± 0.02 ^b 8.94 ± 0.05 ^c 2.56 ± 0.07 ^c Example 2 2.49 ± 0.01 ^b 3.05 ± 0.02 ^b 9.78 ± 0.34 ^b 2.80 ± 0.09 ^b Example 3 2.66 ± 0.01 ^a 3.29 ± 0.03 ^a 12.24 ± 0.41 ^a 3.33 ± 0.09 ^a 1) The result is the average of 3 measurements average ± standard deviation. Values with different characters in the same column are significantly different (p <0.05).
2) D _[4,3] : Volume-weight average diameter
3) D ₅₀ : Median particle diameter of cumulative volume distribution (50th percentile)
4) D ₉₀ : 90th percentile of the cumulative volume distribution

As shown in Table 2, in the case of Examples 1 to 3 including the root sheath polysaccharide as the secondary coating material, the D _[4,3] value was remarkably high. In addition, the value of D _[4,3] increased significantly as the concentration of root muscle polysaccharide increased in the examples.

The increase in the particle size of the double microcapsules of Examples 1 to 3 may be attributed to the formation of a thicker coating layer due to the electrostatic interaction between the anionic reactive site of the root muscle polysaccharide and the cationic site of whey protein.

1-4. Of double microcapsules  Efficiency measurement

The Folin-Ciocalteu method was carried out to measure the encapsulation efficiency of the comparative example and the double microcapsules of Examples 1 to 3 in which the type and / or content of the secondary coating material were differently prepared. The encapsulation efficiency means the degree to which the coating material stably protects the core material, and was measured through the percentage of gallic acid, which is the core material used in the double microcapsule of the present invention (Table 3).

division Encapsulation efficiency (%) Gallic acid concentration (ug / g dry powder) Comparative example 78.53 ± 1.03 ^c 793.11 ± 0.26 ^d Example 1 80.20 ± 0.34 ^b 806.22 ± 0.27 ^c Example 2 81.03 ± 0.32 ^b 809.06 ± 0.23 ^b Example 3 82.64 ± 0.81 ^a 824.99 ± 0.03 ^a

* Values with the same alphabetic character in the same column show no significant difference (p <0.05)

As shown in Table 3, Examples 1 to 3 exhibited superior encapsulation efficiency compared to Comparative Examples, and in Examples 1 to 3, the encapsulation efficiency increased significantly as the content of the root carcass polysaccharide increased. It can be said that the encapsulation efficiency is improved by electrostatic attraction between the root muscle polysaccharide and whey protein, and through the above results, it was confirmed that the double microcapsule of the present invention is excellent in stably protecting the central material gallic acid.

1-5. Of double microcapsules  Moisture content measurement

The moisture content of the double microcapsules is related to properties such as drying efficiency, powder flowability, adhesion and storage stability according to the vitrification and crystallization of the capsule. Accordingly, the moisture content of the double microcapsules of Comparative Examples and Examples 1 to 3 in which the type and / or content of the secondary coating material was differently prepared was measured. Specifically, after drying the double microcapsules of Comparative Examples and Examples 1 to 3 in a hot air oven at 105 ° C, it was calculated as the ratio of the weight reduced by drying to the total weight of the double microcapsules (Table 4.)

division Water content (%) Comparative example 2.64 ± 0.07 ^a Example 1 0.83 ± 0.51 ^b Example 2 0.43 ± 0.21 ^bc Example 3 0.30 ± 0.10 ^c

* Values with the same alphabetic character in the same column show no significant difference (p <0.05)

As shown in Table 4, the water content of the double microcapsules was significantly lower in Examples 1 to 3 than the comparative example, and in Examples 1 to 3, the water content of the double microcapsules was increased as the concentration of the root muscle polysaccharide increased. This decreased significantly.

In general, when the water content of the double microcapsules is high, the coating material changes the crystallization of the capsule, so that the core material can be released or decomposed during the storage period, resulting in low storage stability. Accordingly, the results indicate that the storage stability of the core material can be significantly maximized by lowering the moisture content of the double microcapsules by including the root skin polysaccharide in the secondary coating material.

1-6. Fourier transform infrared (FT-IR) measurement

FT-IR was measured in order to confirm the interaction between the secondary coating material, root muscle polysaccharide and whey protein. Specifically, a Combined Raman FTIR spectrophotometer (LabRam Aramis IR2, Horiba Jobin Yvon) connected to a total reflectance attenuator (FTIRATR) was used. Samples of Comparative Examples and Examples 1 to 3 double microcapsules were dried in a drying oven for 5 hours, and scanned at a range of 650 to 4000 cm ^-1 at a resolution of 8 cm ^-1 at room temperature. As a result, it was confirmed that a new peak of 1,577 cm ^-1 appeared in Examples 1 to 3 (Fig. 4).

These results indicate that a new amide bond was formed between the amino group of the whey protein and the carboxyl group of the root muscle polysaccharide during spray drying of the complex of the root muscle polysaccharide and whey protein.

Experimental example  2. Of double microcapsules  Storage stability check

The storage stability with respect to the core material was measured while storing the double microcapsules of Comparative Examples and Examples 1 to 3, which had different types and / or contents of the secondary coating material, in an incubator set at various temperatures for 20 days. Specifically, the MIR-153 incubator of Comparative Example and Examples 1 to 3 double microcapsules (0.1 g) of 4 ℃ (A), 20 ℃ (B) and 40 ℃ (C), respectively (Sanyo, Gunma, Japan) And the storage stability for the core material was measured every 5 days for 20 days. Storage stability of the core material at different temperatures is known in the conventional method (Lee, YK, Ganesan, P., Baharin, BS, & Kwak, HS (2015) .Characteristics, stability, and release of peanut sprout extracts in powdered microcapsules by spray drying.Drying Technology , 33, 1991-2001.) (Fig. 5).

As shown in Fig. 5, Examples 1 to 3 at all storage temperatures were superior in storage stability compared to Comparative Examples, and in particular, Example 3, which had the highest content of lactose polysaccharide, showed that storage stability was remarkably excellent.

The above results suggest that the double microcapsules (Examples 1 to 3) coated with a complex of root muscle polysaccharide and whey protein significantly retard the release of the core material than the double microcapsules coated with whey protein (Comparative Example).

Experimental Example 3. Confirmation of digestion stability of double microcapsules

The digestion stability of the double microcapsules was measured by measuring the release amount of gallic acid, the core material, after treating under the composition of artificial gastric juice in the double microcapsules of Comparative Examples and Examples 1 to 3 in which the type and / or content of the secondary coating material were differently prepared. Confirmed. Specifically, 0.2 g of the double microcapsules of Comparative Examples and Examples 1 to 3 were suspended in 20 mL distilled water, and then the pH of the suspension was adjusted to pH 2.0 using 0.1 M HCl, followed by 0.25 g of pepsin solution (1 g / 0.1 M HCl 25 mL) was added to form an artificial gastric juice. Thereafter, the mixture was stirred at 75 rpm in a 37 ° C. magnetic stirring water bath and the release amount of gallic acid was measured at regular intervals for a total of 2 hours (FIG. 6).

As a result of the measurement, the release amount of gallic acid after 2 hours in artificial gastric juice of Comparative Examples and Examples 1 to 3 was 8.09, 4.57, 3.47, and 2.87%, respectively, and the release amount of gallic acid, a central substance, increased the concentration of the root muscle polysaccharide. It was confirmed to be lowered according to (Fig. 6). It can be seen that it is difficult to release the core material inside the double microcapsules in acidic conditions containing pepsin as the double microcapsules coated with the root muscle polysaccharide and whey protein complex form aggregates and precipitate from the solution.

In addition, the complex of anionic polysaccharide and cationic protein (pH <pI) exhibits strong electrical cell interaction, which can induce the formation of strong electrical cell interaction between the protein and the polysaccharide under gastric conditions of acidic pH. . Therefore, the digestion stability of the double microcapsule made of a complex of whey protein and whey protein with electrostatic interaction is enhanced, and the stability of gallic acid, a core material, is further improved.

The above results suggest that the core material of the double microcapsules can be protected from the stomach through the secondary coating material containing the root carcass polysaccharide, thereby increasing the absorption in the small intestine and increasing the bioavailability. do.

The above description of the present invention is for illustration only, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present invention.

Claims (12)

The core material containing gallic acid and the core material sequentially coated with a primary coating material and a secondary coating material, a double fine capsule. According to claim 1,
The primary coating material is a medium-chain triglyceride (MCT) medium and polyglycerol polyricinoleate (PGPR, polyglycerol polyricinoleate), a double microcapsule. According to claim 1,
The secondary coating material will include the root muscle polysaccharides, double microcapsules. According to claim 1,
The secondary coating material is a double microcapsule that contains 1.0% by weight to 2.0% by weight of the root carcass polysaccharide. According to claim 1,
The secondary coating material is to include a complex of milky root polysaccharides and whey proteins, double microcapsules. According to claim 1,
The secondary coating material is a double microcapsule comprising a complex composed of a ratio of 1: 15 to 1: 30 based on the weight percent of root oil polysaccharide and whey protein. a) preparing a primary emulsion by mixing a central material containing gallic acid and a primary coating material; And
b) A method of manufacturing a double microcapsule, comprising the step of preparing a second emulsion by mixing the first emulsion and a second coating material. The method of claim 7,
The primary coating material is a mixture of medium chain triglyceride (MCT) oil and polyglycerol polyricinoleate (PGPR, polyglycerol polyricinoleate). The method of claim 7,
The secondary coating material is to include the root muscle polysaccharide, a method for producing a double microcapsules. The method of claim 7,
Wherein the secondary coating material is a root micropolysaccharide containing 1.0% to 2.0% by weight of the double microcapsules. The method of claim 7,
The secondary coating material comprises a complex of milky root polysaccharide and whey protein, a method for producing a double microcapsule. The method of claim 7,
The secondary coating material is a root micropolysaccharide and whey protein containing a complex composed of a ratio of 1: 15 to 1: 30 based on weight percent, a method for producing a double microcapsule.

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