CN107875038B - Olive oil microemulsion preparation and application thereof as whitening sunscreen agent - Google Patents

Abstract

The invention relates to an olive oil microemulsion preparation and application thereof as a whitening sun-screening agent, wherein the olive oil microemulsion preparation comprises the following components in percentage by mass: olive oil: 20-35%; rutin: 5-8%; soybean lecithin: 15-20%; span-80: 8-10%; anhydrous ethanol: 15-18%; glycerol: 5-8%; the balance being deionized water.

Description

Olive oil microemulsion preparation and application thereof as whitening sunscreen agent

Technical Field

The invention belongs to the field of whitening sun protection, and particularly relates to an olive oil microemulsion preparation and application thereof as a whitening sun protection agent.

Background

The microemulsion is an isotropic and thermodynamically stable dispersion system formed by oil, water, a surfactant and a cosurfactant spontaneously, the particle size of liquid drops is about 10-100nm, and the microemulsion is pure in appearance, excellent in stability, good in skin feel and efficient in drug carrying property and is widely applied to the fields of medicines, foods, cosmetics and the like. The microemulsion is helpful for improving the solubility of active substances and improving the stability of substances which are easy to oxidize and decompose. In addition, the microemulsion can realize the targeting of the active ingredients in the human digestive tract environment, thereby achieving the slow release and controlled release, enabling the nutrient ingredients to be more easily absorbed and utilized and improving the bioavailability thereof. However, cosmetic-grade microemulsions have high requirements for components such as surfactants, and the type and amount of the surfactant are strictly controlled from the viewpoint of safety.

Olive oil is known as "liquid gold" because of its unique flavor, rich nutrients and active ingredients, and has the health-care functions of promoting bone growth and development of nervous system, beautifying face, resisting senility and improving digestive system function. In addition, it also has effects of preventing cardiovascular diseases, preventing cancer, and preventing diabetes and Alzheimer's disease. Because the olive oil mainly consists of long-chain fatty acid and has larger molecular weight, the microemulsion which is uniform, stable and has certain water dilutability is not easy to form.

Rutin, also called rutin, is a flavonoid bioactive substance, and has the effects of maintaining and enhancing capillary resistance, reducing permeability, promoting cell proliferation, resisting inflammation, resisting allergy, promoting urination, reducing blood lipid, etc.

The rutin molecular structure has two stronger conjugated systems, so the rutin sunscreen composition has stronger ultraviolet absorption performance and is an ideal natural broad-spectrum sunscreen agent.

The soybean lecithin is an essence extracted from soybeans, and has certain emulsibility and dispersibility due to the fact that a molecular structure contains both hydrophilic groups and hydrophobic groups, and is a pure natural surfactant.

The invention provides a formula of a cosmetic-grade olive oil microemulsion preparation, which realizes multiple effects of sun protection, oxidation resistance, inflammation resistance and the like by adding rutin which is a natural plant extract, and is expected to be developed and used for preparing a whitening sun-screening agent.

Disclosure of Invention

The invention provides an olive oil microemulsion preparation, which is characterized by comprising the following components in percentage by mass: olive oil: 20-35%; rutin: 5-8%; soybean lecithin: 15-20%; span-80: 8-10%; anhydrous ethanol: 15-18%; glycerol: 5-8%; the balance being deionized water.

The invention provides a preparation method of the olive oil microemulsion preparation, which is characterized by comprising the following steps:

(1) putting soybean lecithin, span-80, rutin and absolute ethyl alcohol into a container, uniformly stirring at 50-55 ℃, and carrying out ultrasonic treatment for 3-5min to obtain a uniformly mixed solution A;

(2) mixing olive oil and glycerol to obtain solution B;

(3) and (3) mixing the solution A obtained in the step (1) and the solution B obtained in the step (2) with deionized water at room temperature, and performing high-speed shearing for 5-10 min by using a high-speed shearing machine, wherein the shearing speed is 5000-8000 rpm, so as to obtain the olive oil microemulsion preparation.

The percentage of the dosage of the olive oil, the rutin, the soybean lecithin, the span-80, the absolute ethyl alcohol, the glycerol and the deionized water in the total feeding mass of the microemulsion preparation is that the olive oil: 20-35%; rutin: 5-8%; soybean lecithin: 15-20%; span-80: 8-10%; anhydrous ethanol: 15-18%; glycerol: 5-8%; the balance being deionized water.

Another embodiment of the invention provides an application of the olive oil microemulsion preparation in preparing a whitening sunscreen agent.

Another embodiment of the present invention provides the use of the above olive oil microemulsion preparation for enhancing the ultraviolet absorption effect of a whitening sunscreen agent.

The invention discloses a whitening sun-screening agent, which is characterized by comprising an olive oil microemulsion preparation.

Compared with the prior art, the invention has the advantages that: (1) the olive oil microemulsion preparation has strong absorption capacity on ultraviolet rays (UVA and UVB wave bands), the absorption capacity on the ultraviolet rays (UVA and UVB wave bands) is superior to that of rutin, and the absorption capacity on the UVA is superior to that of a sun-screening agent (4-methylbenzylidene camphor pills), so that the olive oil microemulsion preparation can be developed and used for preparing a whitening sun-screening agent; (2) the olive oil microemulsion is emulsified by utilizing the natural surfactant, the nonionic surfactant and the alcohol, the stability of the olive oil microemulsion is ensured by the screened emulsifier in composition and proportion, the emulsifying characteristic of the olive oil is effectively improved, the prepared olive oil microemulsion is golden yellow, clear and transparent, the particle size is about 10-100nm, and the prepared olive oil microemulsion has high centrifugal stability and storage stability; (3) the method for preparing the olive oil microemulsion preparation has the advantages of simple steps, easiness in operation and low equipment requirement, and therefore, has a wide application prospect.

Drawings

FIG. 1 TEM image of product A

FIG. 2 microemulsion particle size distribution diagram of product A

FIG. 3 TEM image of product I

FIG. 4 particle size distribution diagram of product I

Detailed Description

In order to facilitate a further understanding of the invention, the following examples are provided to illustrate it in more detail. However, these examples are only for better understanding of the present invention and are not intended to limit the scope or the principle of the present invention, and the embodiments of the present invention are not limited to the following.

The preparation method comprises the following steps:

(1) putting soybean lecithin, span-80, rutin and absolute ethyl alcohol into a container, uniformly stirring at 50-55 ℃, and carrying out ultrasonic treatment for 3-5min to obtain a uniformly mixed solution A;

(2) mixing olive oil and glycerol to obtain solution B;

(3) preparing colostrum: and (3) mixing the solution A obtained in the step (1) and the solution B obtained in the step (2) with deionized water at room temperature, and performing high-speed shearing for 5-10 min by using a high-speed shearing machine, wherein the shearing speed is 5000-8000 rpm, so as to obtain the olive oil microemulsion preparation.

Example 1

Olive oil: 35g of a soybean milk powder; rutin: 5g of the total weight of the mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 12g of a mixture; according to the preparation method, the olive oil microemulsion preparation (hereinafter referred to as product A) is prepared.

Example 2

Olive oil: 35g of a soybean milk powder; rutin: 8g of the total weight of the mixture; soybean lecithin: 20g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 8g of the total weight of the mixture; deionized water: 1g of a compound; according to the preparation method, the olive oil microemulsion preparation (hereinafter referred to as product B) is prepared.

Example 3

Olive oil: 20g of the total weight of the mixture; rutin: 8g of the total weight of the mixture; soybean lecithin: 20g of the total weight of the mixture; span-80: 8g of the total weight of the mixture; anhydrous ethanol: 15g of the total weight of the mixture; glycerol: 8g of the total weight of the mixture; deionized water: 21g of a basic amine; according to the preparation method, the olive oil microemulsion preparation (hereinafter referred to as product C) is prepared.

Example 4

Olive oil: 20g of the total weight of the mixture; rutin: 5g of the total weight of the mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 8g of the total weight of the mixture; anhydrous ethanol: 15g of the total weight of the mixture; glycerol: 5g of the total weight of the mixture; deionized water: 32g of a mixture; according to the preparation method, the olive oil microemulsion preparation (hereinafter referred to as product D) is prepared.

Example 5

Olive oil: 30g of the total weight of the mixture; rutin: 6g of a mixture; soybean lecithin: 18g of a mixture; span-80: 9g of a mixture; anhydrous ethanol: 16g of a mixture; glycerol: 6g of a mixture; deionized water: 15g of the total weight of the mixture; according to the preparation method, the olive oil microemulsion preparation (hereinafter referred to as product E) is prepared.

Example 6

Olive oil: 35g of a soybean milk powder; rutin: 2g of the total weight of the mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 15g of the total weight of the mixture; the product F is prepared according to the preparation method.

Example 7

Olive oil: 35g of a soybean milk powder; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 17g of a basic amine; the product G is prepared according to the preparation method.

Example 8

Olive oil: 35g of a soybean milk powder; rutin: 10g of a mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 7g of a mixture; the product H is prepared according to the preparation method.

Example 9

Peanut oil: 35g of a soybean milk powder; rutin: 5g of the total weight of the mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 12g of a mixture; the product I is prepared according to the preparation method.

Example 10

Soybean oil: 35g of a soybean milk powder; rutin: 5g of the total weight of the mixture; soybean lecithin: 15g of the total weight of the mixture; span-80: 10g of a mixture; anhydrous ethanol: 18g of a mixture; glycerol: 5g of the total weight of the mixture; deionized water: 12g of a mixture; the product J is prepared according to the preparation method.

Example 11 testing of the olive oil microemulsion formulation according to the invention for the absorption of the ultraviolet in the UVA and UVB bands

Respectively weighing 10mg of each of the products A-J prepared in the embodiments 1-10, respectively dissolving the products A-J in 25mL of absolute ethyl alcohol to prepare a solution with the concentration of 400 mu g/mL, respectively taking 1mL of the solution out of the solution, and diluting the solution to prepare a solution with the concentration of 40 mu g/mL; preparing rutin and sunscreen agent (4-methylbenzylidene camphor ball) ethanol solution with concentration of 40 μ g/ml by the same method; the solutions prepared above were subjected to ultraviolet spectrometry, and absorbance values were recorded, with the results shown in table 1.

TABLE 1

The experimental result shows that when the olive oil microemulsion preparation does not contain rutin, the olive oil microemulsion preparation almost has no absorption capacity on ultraviolet rays in UVA and UVB wave bands; when the product contains 5-8% of rutin, the absorption capacity of the product to ultraviolet rays in UVA and UVB bands is remarkably enhanced and is far higher than that of pure rutin which is equivalent to the amount of microemulsion to the ultraviolet rays, and the absorption capacity of the product to the UVA is higher than that of a sun-screening agent; when the content of rutin is less than 5%, the absorption capacity of rutin to ultraviolet rays is greatly reduced, and when the content of rutin is more than 8%, the absorption capacity of rutin to ultraviolet rays is not obviously increased. When soybean oil or peanut oil is used to replace olive oil, the obtained microemulsion cannot achieve the effect of the olive oil microemulsion, probably because the olive oil adopted by the invention forms the best match with other components, so that the whole microemulsion is changed in the aspect of physical and chemical properties, and the absorption capacity of the microemulsion on ultraviolet rays is improved. Therefore, when the rutin content in the olive oil microemulsion preparation is 5-8%, the absorption effect on ultraviolet rays is optimal. The olive oil microemulsion preparation prepared by the invention can be used for preparing whitening sunscreen agents.

Claims (2)

1. The application of an olive oil microemulsion preparation in preparing a whitening sun-screening agent comprises the following components in percentage by mass: olive oil: 20-35%; rutin: 5-8%; soybean lecithin: 15-20%; span-80: 8-10%; anhydrous ethanol: 15-18%; glycerol: 5-8%; the balance of deionized water;

the preparation method comprises the following steps:

(1) putting soybean lecithin, span-80, rutin and absolute ethyl alcohol into a container, uniformly stirring at 50-55 ℃, and carrying out ultrasonic treatment for 3-5min to obtain a uniformly mixed solution A;

(2) mixing olive oil and glycerol to obtain solution B;

(3) and (3) mixing the solution A obtained in the step (1) and the solution B obtained in the step (2) with deionized water at room temperature, and performing high-speed shearing for 5-10 min by using a high-speed shearing machine, wherein the shearing speed is 5000-8000 rpm, so as to obtain the olive oil microemulsion preparation.

2. The whitening sunscreen agent is characterized by comprising an olive oil microemulsion preparation, wherein the olive oil microemulsion preparation comprises the following components in percentage by mass: olive oil: 20-35%; rutin: 5-8%; soybean lecithin: 15-20%; span-80: 8-10%; anhydrous ethanol: 15-18%; glycerol: 5-8%; the balance of deionized water;

the preparation method comprises the following steps:

(1) putting soybean lecithin, span-80, rutin and absolute ethyl alcohol into a container, uniformly stirring at 50-55 ℃, and carrying out ultrasonic treatment for 3-5min to obtain a uniformly mixed solution A;

(2) mixing olive oil and glycerol to obtain solution B;

(3) and (3) mixing the solution A obtained in the step (1) and the solution B obtained in the step (2) with deionized water at room temperature, and performing high-speed shearing for 5-10 min by using a high-speed shearing machine, wherein the shearing speed is 5000-8000 rpm, so as to obtain the olive oil microemulsion preparation.

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