KR20190099959A - Composition for Preventing, Improving or Treating of muscular disease containing Codium SPP. algae extract - Google Patents

Abstract

The present invention relates to a composition for preventing, alleviating or treating muscular diseases containing a Codium spp. marine algae extract. More specifically, the present invention relates to a pharmaceutical composition for preventing or treating muscular diseases and a food composition for preventing or alleviating muscular diseases containing the Codium spp. marine algae extract as an active component. The composition containing the Codium spp. marine algae extract of the present invention can not only be usefully used for preventing, alleviating or treating muscular diseases, but also can be usefully used as the food composition for enhancing exercise ability and a composition for strengthening muscles.

Description

Composition for Preventing, Improving or Treating muscular disease containing Codium SPP. algae extract}

The present invention relates to a composition for preventing, ameliorating or treating muscle diseases comprising an extract of seaweed in the corium, and more particularly, a pharmaceutical composition for preventing or treating muscle diseases comprising the extract of seaweed in the corium as an active ingredient, It relates to a food composition for prevention or improvement.

Skeletal muscles make up the largest part of the body, accounting for 40-50% of the total body weight, and play an important role in various metabolic functions in the body, including energy homeostasis and heat generation. Muscle size is controlled by intracellular signaling pathways that induce anabolism or catabolism in muscle. If more signaling reactions that induce synthesis occur than muscle protein breakdown, muscle protein synthesis is increased, resulting in hypertrophy or hyperplasia, which increases muscle size. Journal of Sports Science, 20 (3): 1551-1561, 2011).

Human muscle decreases by more than 1% per year after age 40, and by age 80, 50% of the maximum muscle mass is reduced, and muscle loss in old age is recognized as one of the most important factors that impair overall physical function. As aging progresses, the body shape changes such as muscle and fat content and skeletal distortion. Obesity prevalence due to the decrease in old age has been continuously increasing at the level of more than 30% worldwide. Abnormal insulin secretion can cause muscle development problems due to poor energy supply to cells, leading to an increase in myopathy in diabetics than the general population. In addition, a decrease in muscle causes more arthritis, back pain, and chronic pain, and may also worsen urinary incontinence caused by abdominal obesity, and injuries due to fractures may lead to death and increase death in old age. Muscular dystrophy is a major cause of poor quality of life associated with various diseases. Muscular dystrophy is known to be closely related to senile chronic diseases such as osteoporosis, insulin resistance, and arthritis, and can prevent a decrease in physical activity due to aging through prevention or improvement of myopathy.

The global market for progressive ataxia and muscle weakness treatments amounted to about $ 14 billion in 2011, and is expected to grow to $ 23.5 billion in 2017, with a compound annual growth rate of 9.4%. Increasing mitochondrial production, muscle protein degradation inhibitory, anti-inflammatory drugs, etc. have been suggested as a treatment for muscle reduction, but there is no clear drug.

In addition, the proposed diet to prevent myopathy requires 25-30 g of high quality protein per meal. This means that 4-5 eggs or about 120 g of chicken breasts should be consumed every meal. Therefore, although many people have recently selected protein supplements as an alternative, protein supplements can cause excessive protein intake and have a high potential for side effects. In addition, if you have a kidney disease can not diet high protein and kidney function also decreases with aging, so other alternatives to high protein intake is required to prevent myopathy.

Therefore, the present inventors conducted a study using various natural materials to develop a composition for strengthening muscle, and as a result, MHC binding index and mRNA expression in auditory extracts of seaweeds in the codium (Codium) were myoblasts and myotubes. The present invention was completed by confirming the effect of increasing the diameter of the root canal cells.

Accordingly, an object of the present invention is to provide a composition for preventing, improving or treating muscle diseases, which comprises an algae extract of the genus Codium as an active ingredient.

In addition, another object of the present invention to provide a composition for strengthening muscles comprising a seaweed extract of the genus (Codium) as an active ingredient.

In addition, another object of the present invention is to provide a food composition for improving athletic performance comprising an extract of seaweed in the genus (Codium) as an active ingredient.

In order to achieve the above object, the present invention provides a composition for the prevention, improvement or treatment of muscle diseases comprising a seaweed extract of the genus (Codium) as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a composition for muscle strengthening comprising an extract of seaweed in the genus (Codium) as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a food composition for improving athletic performance comprising an extract of seaweeds of the genus (Codium) as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for preventing, improving or treating muscle diseases, which comprises an algae extract of the genus Codium as an active ingredient.

The composition according to the present invention may be a pharmaceutical composition or a food composition, the pharmaceutical composition may be a composition comprising a seaweed extract of the genus Cordium as an active ingredient, a composition consisting of a seaweed extract of the genus Cordium as an active ingredient It may be, or may be a composition consisting essentially of seaweed extract of the cordium as an active ingredient.

As used herein, the term 'comprising' is used in the same sense as 'including' or 'characterized by', and specifically, in the composition or method according to the present invention, Does not exclude additional components or steps of the method and the like that are not mentioned. In addition, the term 'consisting of' means to exclude additional elements, steps, or components that are not separately described. The term 'essentially consisting of' means that in the scope of the composition or method, it may include the substances or steps described, as well as the substances or steps which do not substantially affect the basic properties thereof. .

The “codium seaweed” of the present invention is a green algae group and is widely distributed all over the world, and is widely used for food in Korea, China, Japan, as well as the Philippines and Hawaii. It contains a lot of sugar, protein, and fiber. It contains a lot more minerals than land plants, so it is rich in calcium, potassium and iron. The height is 10-30cm, the thickness is 1.5-3mm, and the lower side is thicker. The branches are split into deer horns, grow straight, and reach the same height, forming a fan shape. The surface is smooth like a velvety, and under a microscope there is an irregular tangled texture of colorless transparent threads inside the branches. Young ones have hairs on the front, especially on top, but fall later and leave hairless marks. The whole body is a structure in which several cell nuclei are contained in one cell because they are all connected without a membrane between cells. Spore containing pouches are club-shaped, 5-7 times the thickness, the top is sharp. In the past, it was used as a roundworm medicine because of the antiparasitic component, and the water-soluble extract is known to have strong antibiotic action against bacteria.

In the present invention, the algae in the corium is not limited thereto, but may be any one selected from the group consisting of hearing (Codium fragile), Mongwoo auditory (Codium contractum), broad hearing (Codium latum) and supine hearing (Codium coactum). .

In the present specification, 'muscle' refers to tendons, muscles, and tendons in general, and 'muscle function' means the ability to exert strength by contraction of muscles, and the muscles exert maximum contraction force to overcome resistance. Muscle endurance, the ability to express how long or how many times a muscle can repeat contraction and relaxation at a given weight, and quickness, the ability to exert a strong force in a short time. This muscle function is dominated by the liver and is proportional to muscle mass.

"Muscle disease" of the present invention means a disease caused by muscle wasting or degeneration. Examples of such diseases include sarcopenia, atony, muscular atrophy, muscular dystrophy, muscle degeneration, myopathy, muscular dystrophy, muscular atrophy, myasthenia and cachexia Can be mentioned. Muscle wasting is characterized by gradual loss of muscle mass, weakness and degeneration of muscles, particularly skeletal or veterinary and cardiac muscle. Muscle wasting and degeneration are caused by genetics, acquired factors, and aging. The composition of the present invention has a muscle increasing effect, the muscle does not limit its kind. Muscle gain is to improve the performance of the muscles, especially among the body components, can increase the muscle mass through physical exercise and endurance improvement, and can increase the muscle mass by administering a substance having a muscle increasing effect in the body.

The composition for preventing, improving or treating muscle diseases of the present invention may contain a seaweed extract of the genus Cordium alone or may contain one or more active ingredients exhibiting a function similar to the extract of the seaweed of the genus Cordium. Inclusion of additional components may further enhance the muscle disease improving effect of the composition of the present invention. When the ingredient is added, skin safety, ease of formulation, and stability of the active ingredients may be considered.

Corium algae extract of the present invention may include a pretreatment process such as a drying process for the raw material in order to increase the extraction efficiency in the preparation of the extract.

Corium algae extract of the present invention can be extracted by a known natural product extraction method. Preferably it may be extracted with one or more solvents selected from the group consisting of water, organic solvents having 1 to 6 carbon atoms and subcritical or supercritical fluids. The organic solvent having 1 to 6 carbon atoms has 1 to 6 carbon atoms (alcohol), acetone (acetone), ether (ether), benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride, hexane, cyclohexane, and petroleum ether, but is not limited thereto.

In addition, the seaweed extract of the cordium of the present invention can be obtained by extracting and purifying the seaweeds of the cordium using purified water, ethanol and subcritical water or supercritical carbon dioxide suitable for food processing, or by directly compressing the seaweeds in the cordium It can be obtained by separating and purifying from the oil thus obtained. For example, the extract can be obtained by extracting algae in the cordium under ultra-high pressure conditions of 100 Mpa or more. Preferably, it may be an ultrahigh pressure condition of 100 MPa to 1000 MPa, but is not limited thereto.

The extract of the present invention may be used as a liquid by filtration and / or concentration, and may be used by solidifying through a conventional drying process such as spray drying or lyophilization. In the drying process, it may be dried by mixing the spray drying or dextrin before lyophilization.

Corium algae extract of the present invention can be used as an effective component of the pharmaceutical composition because it has an excellent effect of increasing the diameter of the muscle cells.

In addition, the algae extract of the genus Cordium of the present invention has an excellent effect of increasing the diameter of the muscle cells can be used as an effective component of the food composition.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention may further include a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may further include, for example, carriers for oral administration or carriers for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid and the like. Carriers for parenteral administration may also include water, suitable oils, saline, aqueous glucose, glycols, and the like. In addition, stabilizers and preservatives may be further included. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. Other pharmaceutically acceptable carriers may be referred to those described in the following documents (Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995).

The pharmaceutical composition of the present invention can be administered to any mammal, including humans. For example, it can be administered orally or parenterally, parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intradural, intracardiac, transdermal, subcutaneous, intraperitoneal , Intranasal, intestinal, topical, sublingual or rectal administration.

The pharmaceutical composition of the present invention may be formulated into a preparation for oral or parenteral administration according to the route of administration as described above. When formulated, one or more buffers (e.g. saline or PBS), antioxidants, bacteriostatic agents, chelating agents (e.g. EDTA or glutathione), fillers, extenders, binders, adjuvants (e.g. aluminum hydroxide) Side), suspending agents, thickening humectants, disintegrants or surfactants, diluents or excipients.

Solid form preparations for oral administration include tablets, pills, powders, granules, solutions, gels, syrups, slurries, suspensions, or capsules, and the like may include at least one excipient in the pharmaceutical composition of the present invention. For example, starch (including corn starch, wheat starch, rice starch, potato starch, etc.), calcium carbonate, sucrose, lactose, dextrose, sorbitol, mannitol, xylitol, erythritol maltitol, It can be prepared by mixing cellulose, methyl cellulose, sodium carboxymethyl cellulose and hydroxypropylmethyl-cellulose or gelatin. For example, tablets or dragees can be obtained by combining the active ingredients with solid excipients and then grinding them and adding suitable auxiliaries and then processing them into granule mixtures.

In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Liquid preparations for oral use include suspensions, solutions, emulsions, or syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, or preservatives, in addition to water or liquid paraffin, which are commonly used simple diluents. .

In addition, in some cases, crosslinked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may be added as a disintegrant, and may further include an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, a preservative, and the like. .

When administered parenterally, the pharmaceutical compositions of the present invention may be formulated according to methods known in the art in the form of injections, transdermal and nasal inhalants with suitable parenteral carriers. Such injections must be sterile and protected from contamination of microorganisms such as bacteria and fungi. Examples of suitable carriers for injections include, but are not limited to, solvents or dispersion media comprising water, ethanol, polyols (e.g., glycerol, propylene glycol and liquid polyethylene glycols, etc.), mixtures thereof and / or vegetable oils Can be. More preferably, suitable carriers include Hanks solution, Ringer's solution, phosphate buffered saline (PBS) containing triethanol amine or sterile water for injection, 10% ethanol, 40% propylene glycol and 5% dextrose Etc. can be used. In order to protect the injection from microbial contamination, various antibacterial and antifungal agents such as parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like may be further included. In addition, the injection may in most cases further comprise an isotonic agent such as sugar or sodium chloride.

In the case of transdermal administrations, ointments, creams, lotions, gels, external preparations, pasta preparations, linen preparations, air rolls and the like are included. As used herein, "transdermal administration" means that the pharmaceutical composition is applied to the skin topically so that an effective amount of the active ingredient contained in the pharmaceutical composition is delivered into the skin.

For inhalational administrations, the algae extract of the genus Cordium of the present invention is pressurized pack using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. Alternatively, it can be delivered conveniently from the nebulizer in the form of an aerosol spray. In the case of a pressurized aerosol, the dosage unit can be determined by providing a valve to deliver a metered amount. For example, gelatin capsules and cartridges for use in inhalers or blowers can be formulated to contain a mixture of the compound and a suitable powder base such as lactose or starch. Formulations for parenteral administration are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a prescription generally known in all pharmaceutical chemistries.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention may provide a desirable muscle disease prevention and treatment effect when an effective amount of seaweed extract in the cordium is included. In the present specification, the 'effective amount' refers to an amount that exhibits a higher response than the negative control, and preferably refers to an amount sufficient to increase muscle mass or improve muscle function. The pharmaceutical composition of the present invention may contain 0.01 to 99.99% of the algae extract of the cordium, and the remaining amount may be occupied by a pharmaceutically acceptable carrier. The effective amount of the seaweed extract of the genus Cordium included in the pharmaceutical composition of the present invention will vary depending on the form of the composition and the like.

As used herein, "treatment" refers to a clinical procedure to alter the natural process of the individual or cell being treated, and may also be performed for the prevention of clinical pathology. Desirable effects of treatment include: inhibiting the occurrence or recurrence of the disease, alleviating symptoms, reducing any direct or indirect pathological consequences of the disease, decreasing the rate of disease progression, improving the disease state, improving, alleviating or improving the prognosis, and the like. Include. The term "prevention" also means any action that inhibits the onset or delays the progression of a disease.

The total effective amount of the pharmaceutical composition of the present invention may be administered to a patient in a single dose and may be administered by a fractionated treatment protocol which is administered in multiple doses for a long time. . The pharmaceutical composition of the present invention may vary the content of the active ingredient depending on the extent of the disease. When administered parenterally, it is preferably administered in an amount of 0.01 to 50 mg, more preferably 0.1 to 30 mg per kg of body weight per day, based on the seaweed extract in the cordium, and when used orally, the seaweed extract in the cordium It can be administered in divided doses one to several times to be administered in an amount of preferably 0.01 to 100 mg, more preferably 0.01 to 10 mg per kg body weight per day. However, the dose of seaweed extract in the cordium is effective for the patient in consideration of various factors such as the age, weight, health condition, sex, severity of the disease, diet and excretion rate, as well as the route of administration and the number of treatments of the pharmaceutical composition. Since the dosage is determined, one of ordinary skill in the art will be able to determine the appropriate effective dosage for the specific application for the prevention and treatment of muscle disease in the Cordium seaweed extract. The pharmaceutical composition according to the present invention is not particularly limited to its formulation, route of administration and method of administration as long as the effect of the present invention is shown.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy or biological response modifiers.

The pharmaceutical composition for preventing and treating muscle diseases of the present invention may also be provided in a formulation of an external preparation containing an extract of seaweed in the cordium as an active ingredient.

When the pharmaceutical composition for preventing and treating muscle diseases of the present invention is used as an external preparation for skin, it is additionally used as a fatty substance, an organic solvent, a dissolving agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, and a foaming agent. agents), fragrances, surfactants, water, ionic emulsifiers, nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic active agents, lipophilic It may contain adjuvants commonly used in the field of dermatology, such as active agents or any other ingredients commonly used in external preparations for skin, such as lipid vesicles. The ingredients may also be introduced in amounts generally used in the field of dermatology.

When the pharmaceutical composition for preventing or treating muscle diseases of the present invention is provided as an external preparation for skin, it may be a formulation such as, but not limited to, ointment, patch, gel, cream or spray.

The composition for preventing or improving muscle diseases of the present invention may also be a food composition. When the composition for preventing or improving muscle diseases of the present invention is a food composition, it may be used for preventing or improving muscle diseases due to muscle wasting or degeneration. Muscle depletion and degeneration occur due to genetic factors, acquired factors, aging, etc., muscle depletion is characterized by a gradual loss of muscle mass, weakening and degeneration of muscles, especially skeletal or veterinary and cardiac muscles. Examples of related diseases include sarcopenia, atony, muscular atrophy, muscular dystrophy, muscle degeneration, muscular dystrophy, muscular dystrophy, muscular atrophy, ataxia, dystonia and cachexia ), And the like. The muscle does not limit its kind.

The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food, food additives and feed, and includes animals such as humans or livestock. It is for eating. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. General foods include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned foods, jams, marmalade, etc.), fish, meat and processed foods (e.g. hams, sausages) Cornbread, etc.), breads and noodles (e.g. udon, soba noodles, ramen, spagate, macaroni, etc.), fruit juices, various drinks, cookies, malts, dairy products (e.g. butter, cheese), edible vegetable oils, margarine , Plant protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.) can be prepared by adding the above seaweed extract of the genus Cordium. In addition, it is not limited to this as a dietary supplement can be prepared by adding a seaweed extract of the cordium in capsules, tablets, pills and the like. In addition, as a health functional food, but not limited to, for example, liquefied, granulated, encapsulated and powdered so that the algae extract itself in the cordium in the form of tea, juice and drinks to drink (healthy beverages) Can be consumed. In addition, in order to use the seaweed extract in the cordium in the form of a food additive, it can be prepared in powder or concentrate form. In addition, it can be prepared in the form of a composition by mixing with a seaweed extract of the cordium and known active ingredients known to be effective in preventing muscle diseases and improving muscle function.

When the composition for preventing or improving muscle diseases of the present invention is used as a health beverage composition, the health beverage composition may contain various flavors or natural carbohydrates, etc. as additional components, as in general beverages. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Polysaccharides such as dextrin, cyclodextrin; Sugar alcohols such as xylitol, sorbitol, and erythritol. Sweeteners include natural sweeteners such as taumartin, stevia extract; Synthetic sweeteners such as saccharin and aspartame; The proportion of said natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

When the seaweed extract in the cordium is contained as an active ingredient of a food composition for preventing or improving muscle diseases, the amount thereof is not particularly limited to an amount effective to achieve the action for preventing muscle diseases and improving muscle function, but the total weight of the total composition It is preferably from 0.01 to 100% by weight relative to. The food composition of the present invention may be prepared by mixing with the seaweed extract of the cordium with other active ingredients known to be effective in the composition for preventing muscle diseases and improving muscle function.

In addition to the above, the health food of the present invention is various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, Glycerin, alcohol or carbonation agent, and the like. In addition, the health food of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage or vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In another aspect, the present invention provides a composition for muscle strengthening comprising seaweed extract of the genus Codium as an active ingredient.

As used herein, the term "muscle strengthening" means that muscle mass is increased or muscle loss is suppressed.

Coral seaweed extract of the present invention is excellent in the effect of increasing the binding of MHC (Myosin heavy chain) can be used as an effective component of the pharmaceutical composition.

In addition, the seaweed extract of the cordium of the present invention is excellent in the effect of increasing the binding of MHC (Myosin heavy chain) can be used as an effective ingredient of the food composition.

The preparation method of the seaweed extract in the cordium is as described above, carriers that can be included in the pharmaceutical composition comprising the seaweed extract in the cordium as an active ingredient, the formulation of the pharmaceutical composition, the route of administration and dosage, etc. The administration method is as described above.

The pharmaceutical composition according to the present invention may be a composition comprising an algae extract of the genus Cordium as an active ingredient, or may be a composition consisting of an algae extract of the genus Cordium as an active ingredient, or an extract of the algae of Cordium as an active ingredient The composition may consist essentially of this.

Corium algae extract for preparing the food composition is as described above. Moreover, it is as having mentioned above about the illustration and content of the said food composition.

In another aspect, the present invention provides a food composition for improving athletic performance comprising a seaweed extract of the genus (Codium) as an active ingredient.

As used herein, the term 'exercise enhancement' refers to an effect of improving muscle function, such as muscle strength and muscular endurance, preferably muscle strength or muscular endurance may be increased, degenerative disease, mitochondrial disorders, endurance disorder, quickness It may be to prevent or ameliorate one or more symptoms selected from the group consisting of hypothyroidism, lethargy, muscle discard and depression.

Corium algae extract for preparing the food composition is as described above. Moreover, it is as having mentioned above about the illustration and content of the said food composition.

Therefore, the present invention confirmed the effect on the muscle cells of the algae extract of the cordium, the algae extract of the cordium increased the binding index and mRNA expression of MHC in myoblasts and myotubes and increased the diameter of the myotubes Since it can be usefully used as a composition for preventing, improving or treating muscle diseases, food composition for enhancing athletic performance, and composition for strengthening muscles.

1 is a graph showing the binding index (fusion index) and mRNA expression of MHC (myosin heavy chain) according to concentrations when the auditory extract was treated to myoblasts.
Figure 2 shows the differentiation of myoblasts into myotubes and induction of atrophy, the results of confirming the change in the diameter and MHC (myosin heavy chain) mRNA expression according to the concentration when the auditory extract was treated.
Figure 3 shows the result of confirming the diameter of the cells after the treatment of the auditory extract to muscle atrophy-induced myotubes.
4 is a result of confirming the MHC mRNA expression change after the treatment of the auditory extract to muscle atrophy-induced myotube cells.
5 is a graph showing the binding index of MHC when the auditory, mongwoori, wide auditory, supine hearing auditory extracts were treated in the process of differentiating myoblasts into myotubes.

Hereinafter, the present invention will be described in detail.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

<Example 1>

Effect of Auditory Extracts on Differentiation of Muscle Cells

The extract of Codium fragile was purchased from Korea Plant Extract Bank and used as a sample. Each sample is extracted with 95% ethanol or water.

After the hearing extract was treated, the ATP in myosin was decomposed to measure the binding index of the myosin heavy chain (MHC), which is involved in muscle contraction or relaxation (fusion index, number of nuclei in MHC / number of total nuclei).

MHC binding index measurement was performed as follows. C2C12 myoblasts were treated with 1 or 2 μg / ml of auditory extracts and cultured for 2 days, and then fixed with 4% formaldehyde. Permeabilization with 0.1% triton X-100 PBS, then blocked with 5% bovine serum albumin for 1 hour, overnight at 4 ° C. with total MHC antibody, 2 Primary antibody (Alexa Fluor 488-2 secondary antibody, Cell Signaling Biotechnology) was attached for 2 hours at room temperature. After DAPI staining, the images were obtained under a microscope (Olympux IX71). Fusion index was calculated by dividing the number of nuclei in MHC by the total number of nuclei.

As a result, as shown in Figure 1, when the hearing extract was treated compared to the control (untreated) control, the binding index of MHC was found to be high, the binding index was increased with the concentration of the extract .

<Example 2>

Effects of Auditory Extracts on MHC Expression in Muscle Cells

After culturing C2C12 muscle cells, the auditory extracts were treated with 1, 5, 10 μg / mL, incubated for 24 hours, washed the cells with PBS, cells were obtained, and RNA extraction was performed according to the manufacturer's instructions. total RNA was extracted using kit (NucleoSpin RNA plus, MN). Next, cDNA was synthesized by reverse transcription of 1 μg of RNA using 1 mM dNTP and oligo dT (0.5 μg / μl), and then mixed with 1 unit of Tag DNA polymerase, 0.2 mM dNTP, X10 reaction buffer, and 100 pmol of primer. Was made to be 25 μl. PCR was performed on the mixed samples to measure the mRNA expression level of MHC (myosin heavy chain).

As a result, as shown in Figure 2, when the auditory extract was treated compared to the control (untreated) control, the expression level of MHC mRNA was found to increase, the expression level of mRNA was increased according to the concentration of the extract Appeared.

Primer Sequence Sequence SEQ ID NO: MHC2a Sense AAG CGA AGA GTA AGG CTG TC One Anti-sense GTG ATT GCT TGC AAA GGA AC 2 18s RNA Sense GTA ACC CGT TGA ACC CCA TT 3 Anti-sense CCA TCC AAT CGG TAG TAG CG 4

<Example 3>

Effect of Auditory Extracts on Myotubes

Muscle cells were differentiated into root canal cells and then treated with auditory extracts, and changes in cell size and expression of MHC mRNA were confirmed. To this end, C2C12 myoblasts were first differentiated into DMEM containing 2% horse serum for 4 days, and then treated with TNFα 25 ng / ml for 24 hours to induce aging myoatrophy and simultaneously extract auditory extracts 1.0, 5.0 And treated with a concentration of 10.0 μg / ml confirmed the muscle atrophy relief effect.

More specifically, it was performed as follows. Myoblasts from C2C12 mice (CRL1772; American type cell collection, USA) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) medium with 10 (v / v)% FBS and 95% in 6-well plates. After reaching confluence, the cells were transferred to DMEM medium containing 2 (v / v)% horse serum and cultured for 4 days to differentiate into myotubes. Thereafter, TNFα 25 ng / ml was treated for 24 hours to induce aging myotrophy, and at the same time, the auditory extract was treated with 1.0, 5.0, and 10.0 μg / ml concentrations, and then fixed with 4% formaldehyde. Permeabilization with 0.1% triton X-100 PBS, then blocked with 5% bovine serum albumin for 1 hour, overnight at 4 ° C. with total MHC antibody, 2 Primary antibody (Alexa Fluor 488-2 secondary antibody, Cell Signaling Biotechnology) was attached for 2 hours at room temperature. After the DAPI staining, images were obtained under a microscope (Olympux IX71), and the diameter of the myotube was calculated using iSolution software.

As a result, as shown in Figure 3, when treated with TNF-α, the diameter of the cell was reduced. When treated with auditory extracts, the diameter of the cells appeared to increase.

In addition, as a result of confirming the expression change of MHC mRNA in the same manner as in Example 2, as shown in Figure 4, when treated with the auditory extract compared to the case of TNF-α, it appears that the amount of MHC mRNA expression is increased. MRNA expression level increased with the concentration of extract.

<Example 4>

Effects of Seaweed Extracts from Cordium on Myotubes

In addition to hearing, the extracts of the algae in the same cordium, Codium contractum, Codium latum, and Codium coactum extracts were examined on the binding index of MHC in root canal cells. Each extract was purchased from Korea Plant Extract Bank and used as a sample. Each sample was extracted with 95% ethanol or water.

Binding index was measured in the same manner as in Example 1, using muscle cells treated for 48 hours by adding 10 μg / mL of each extract to DMEM containing 2% horse serum.

As a result, as shown in Figure 5, compared with the control (control) untreated, the hearing, Mongwoori hearing, wide hearing and lying hearing when the extract was confirmed, the binding index of MHC was confirmed to increase.

As described above, since the algae extract in the cordium shows an effect of increasing the binding index and mRNA expression of MHC in the myoblasts and the root canal cells and increasing the diameter of the root canal cells, the composition comprising the algae extract of the cordium of the present invention Not only can be usefully used for preventing, improving or treating muscle diseases, but can also be usefully used as a food composition for enhancing athletic ability or a composition for strengthening muscles.

<110> KOREA FOOD RESEARCH INSTITUTE <120> Composition for Preventing, Improving or Treating of muscular          disease containing Codium SPP. algae extract <130> NP17-0117 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MHC2a sense primer <400> 1 aagcgaagag taaggctgtc 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MHC2a anti-sense primer <400> 2 gtgattgctt gcaaaggaac 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 18sRNA sense primer <400> 3 gtaacccgtt gaaccccatt 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 18sRNA anti-sense primer <400> 4 ccatccaatc ggtagtagcg 20

Claims (10)

A composition for the prevention, improvement or treatment of muscle diseases, comprising seaweed extract of the genus (Codium) as an active ingredient.
The composition of claim 1, wherein the extract is an extract by at least one solvent selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, a subcritical fluid, a supercritical fluid, and mixtures thereof.
The method of claim 1, wherein the muscle disease is sarcopenia, atony, muscular atrophy, muscular dystrophy, muscle degeneration, muscular dystrophy, muscular dystrophy, muscular dystrophy, atrophic atrophy And cachexia (cachexia) composition, characterized in that at least one selected from the group consisting of.
Composition for strengthening muscles comprising seaweed extract of the genus (Codium) as an active ingredient.
The composition of claim 4, wherein the muscle strengthening means that an increase in muscle mass or a decrease in muscle is suppressed.
The composition according to any one of claims 1 to 5, wherein the composition is a pharmaceutical composition or a food composition.
According to claim 1 or 4, Codium (Codium) seaweed is selected from the group consisting of hearing (Codium fragile), Coum contractum, Codium latum and Codium coactum The composition, characterized in that any one.
Food composition for improving athletic performance comprising the algae extract of the genus (Codium) as an active ingredient.
The method of claim 8, wherein the exercise ability is a food composition, characterized in that the increase in muscle strength or endurance.
The method of claim 8, wherein the exercise ability improvement is to prevent or improve at least one symptom selected from the group consisting of degenerative diseases, mitochondrial disorders, endurance, improvisation, lethargy, muscle discard and depression, etc. Composition.

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