KR20190090538A - Diagnostic chip for precise detection of optical information in diagnostic samples - Google Patents

Abstract

The present invention relates to a diagnostic chip for precise detection of optical information in a diagnostic sample which, by configuring a color of a boding layer bonding an upper plate and a lower plate constituting the diagnostic ship in black, can minimize a diffuse reflection phenomenon generated from the bonding layer by light irradiated for detecting optical information of the diagnostic sample. For the same, the present invention provides diagnostic chip for precise detection of optical information, which comprises: the upper plate formed with a flow channel through which the diagnostic sample is moved; a lower plate coupled to a lower portion of the upper plate; and the bonding layer formed between the upper plate and the lower plate for bonding the upper plate and the lower plate, wherein the upper plate comprises a diagnostic sample inlet which is connected to one end of the flow channel and into which the diagnostic sample is input, and a diagnostic sample movement control hole connected to the other end of the flow channel to control movement of the diagnostic sample. In addition, the color of the bonding layer is black.

Description

Diagnostic chip for precise detection of optical information in diagnostic samples

The present invention relates to a diagnostic chip for precise detection of optical information of a diagnostic sample, and more particularly, by configuring the color of the bonding layer joining the upper plate and the lower plate constituting the diagnostic chip in black. The present invention relates to a diagnostic chip for precise detection of optical information of a diagnostic sample which minimizes the diffuse reflection phenomenon generated from the bonding layer by light irradiated for information detection.

As the life expectancy of modern people increases, the types of diseases involved also vary, and various diagnostic devices and diagnostic systems have been developed for the prevention and diagnosis of diseases.

One of them, the diagnostic chip, examines or examines the presence of a single or a plurality of substances in a liquid sample, for example a urine or blood sample. Specifically, the modern diagnostic business field is integrated into one of the Point-Of-Care Testing (POCT). POCT is a test that is performed outside a centralized laboratory, and refers to equipment that can be used by the general public without expert knowledge. Currently, diagnostic fields are expanding from hospitals to field and individual.

For example, in a hospital, a patient may be required to take a large amount of antibiotics to fight infections and then take a small amount of blood to investigate whether an adequate amount of antibiotic is present in the blood, In the case of an overdose patient who is injured or unable to communicate, it is possible to quickly investigate the type of medication taken in the human body to ensure proper treatment.

In particular, rapid diagnostic tests, such as immunochromatographic assays, are used to identify diseases or detect changes in the healthcare field, and to quantitatively and quantitatively analyze trace amounts of analytes in a variety of fields, It is being developed in a simple way. In the field of health care, applications are being extended to pregnancy, ovulation, infectious diseases, drug abuse, acute myocardial infarction, and cancer.

As described above, a diagnostic chip having increased demand due to an extended application range is manufactured by various methods. For example, a bonding layer is formed between the upper plate and the lower plate by simply spraying a binder on a surface where the upper plate and the lower plate are coupled. By combining the upper plate and the lower plate thereby to produce a diagnostic chip with a flow channel formed therein.

The diagnostic sample is moved to the flow channel of the fabricated diagnostic chip, and the light is irradiated to the diagnostic chip in order to detect disease by detecting the optical information of the diagnostic sample according to the movement of the diagnostic sample. Detect.

The light irradiated to the diagnostic chip is irradiated to the bonding layer as well as the diagnostic sample. In this case, diffuse reflection may occur from the bonding layer according to the wavelength of the light irradiated to the bonding layer, which causes a problem of increasing the BG (Back groud) value. .

In addition, as the BG value increases due to the diffuse reflection of the bonding layer, a problem of inaccurate accuracy of optical information detected from the diagnostic sample occurs.

Republic of Korea Patent Publication No. 10-0998535 (Invention: Microfluidic circuit device equipped with a microfluidic channel having a nano-gap and a method of manufacturing the same, Announcement date: December 07, 2010)

The present invention minimizes the diffuse reflection phenomenon generated from the bonding layer by the light irradiated for detecting the optical information of the diagnostic sample by configuring the color of the bonding layer joining the upper plate and the lower plate constituting the diagnostic chip in black. The present invention provides a diagnostic chip for precisely detecting optical information of a diagnostic sample.

In order to solve the above problems, the present invention is formed between the upper plate and the lower plate is coupled to the lower portion of the upper plate, the upper plate is formed with a flow channel through which the diagnostic sample is moved, And a bonding layer coupling the upper plate and the lower plate, wherein the upper plate is connected to one end of the flow channel, and includes a diagnostic sample inlet through which the diagnostic sample is input, and is connected to the other end of the flow channel. The diagnostic sample movement control hole is formed to control the movement of the sample, and the bonding layer provides a diagnostic chip for precise optical information detection of the diagnostic sample, characterized in that the color of the black.

In the diagnostic chip for precise detection of diagnostic sample optical information according to the present invention, the color of the bonding layer may be formed by the bonding layer including a liquid dye or a nano pigment.

In the diagnostic chip for accurately detecting the diagnostic sample optical information according to the present invention, the diagnostic chip may further include a sticker layer disposed between the upper plate and the lower plate or on the lower surface of the lower plate, wherein the color of the sticker layer may be black. .

The diagnostic chip for precise optical information detection of the diagnostic sample according to the present invention has the following effects.

First, by forming the color of the bonding layer joining the upper plate and the lower plate constituting the diagnostic chip in black, it is possible to minimize the diffuse reflection phenomenon generated from the bonding layer by the light irradiated for detecting the optical information of the diagnostic sample. There is an advantage to that.

Second, there is an advantage that the optical information of a more accurate diagnostic sample can be detected by reducing the BG value by minimizing the diffuse reflection phenomenon.

1 to 3 are schematic diagrams of a diagnostic chip for precise detection of optical information of a diagnostic sample according to the present invention.
FIG. 4 is a graph illustrating BG values according to colors of a bonding layer included in a diagnostic chip for accurately detecting optical information of the diagnostic sample of FIG. 1.
FIG. 5 is a graph illustrating BG values according to colors of a bonding layer included in a diagnostic chip for precise optical information detection of the diagnostic sample of FIG. 1.
FIG. 6 is a diagram illustrating a graph in which BG values according to colors of a bonding layer included in a diagnostic chip for accurately detecting optical information of the diagnostic sample of FIG. 5 are adjusted based on colorlessness.

Hereinafter, preferred embodiments of the present invention in which the above-mentioned problems to be solved can be specifically realized will be described with reference to the accompanying drawings. In describing the embodiments, the same names and the same symbols are used for the same configurations, and additional description thereof will be omitted in the following.

1 to 6, a diagnostic chip for precise optical information detection of a diagnostic sample according to the present invention will be described.

As shown in Figures 1 to 6, the diagnostic chip for precise optical information detection of the diagnostic sample according to the present invention includes an upper plate 100, lower plate 200 and the bonding layer 300.

The upper plate 100 has a flow channel 400 through which the diagnostic sample S moves, the lower plate 200 is coupled to the lower portion of the upper plate 100, the upper plate ( 100 and the lower plate 200 are formed of a light transmissive material.

In addition, the upper plate 100 and the lower plate 200 is formed of a light transmissive material, the upper plate 100 and the lower plate 200 is formed to have a color, for example the upper plate The color of the 100 and the lower plate 200 may be black.

Since the upper plate 100 and the lower plate 200 are formed in black, the diffuse reflection phenomenon R generated in the bonding layer 300 may be minimized, which will be described later. .

In addition, the upper plate includes a diagnostic sample inlet 110 and the diagnostic sample movement control hole 120.

The diagnostic sample inlet 110 is connected to one end of the flow channel 400 and the diagnostic sample is introduced.

The diagnostic sample movement control hole 120 is connected to the other end of the flow channel 400 to adjust the movement of the diagnostic sample, and equipped with a diagnostic chip for precise detection of optical information of the diagnostic sample according to the present invention. The movement control hole opening and closing unit (not shown) provided in the diagnostic kit may control the movement of the diagnostic sample moving the flow channel 400 by opening and closing the diagnosis sample movement control hole 120.

As shown in FIG. 2, a sample buffer unit 410, a preprocessor 420, a test unit 430, and a reference unit 440 are formed on the flow channel 400, and the sample buffer unit 410 is provided. The preprocessing unit 420, the test unit 430, and the reference unit 440 will be described below.

The sample buffer unit 410 is formed at a position corresponding to an inlet of the diagnostic sample S into which the diagnostic sample S is inserted, in the zero region of the flow channel 400. . Sample buffer material for stabilizing the diagnostic sample (S) is formed in the sample buffer unit 410, and a plurality of sample buffer units 410 may be formed according to the type of the diagnostic sample (S). The sample buffer material may be changed according to the type and target material of the diagnostic sample (S).

The preprocessor 420 is formed at a rear side with respect to the moving direction of the diagnostic sample S from the first region of the flow channel 400, that is, the zero region of the flow channel 400.

The pretreatment unit 420 is formed with a pretreatment material for pretreatment of the diagnostic sample S, which is input from the diagnostic sample S inlet and is buffered and moved in the zero area. The pretreatment unit 420 may be formed in plural numbers, and the pretreatment material may be changed according to the type of the diagnostic sample S and the target material.

The test unit 430 is formed at a rear side with respect to the moving direction of the diagnostic sample S from the second region of the flow channel 400, that is, the first region of the flow channel 400.

The test unit 430 is formed with a test material for testing the diagnostic sample S that is preprocessed and moved in the second region, and irradiates light to the diagnostic sample S that has passed through the test unit 430. Therefore, the disease diagnosis may be performed according to the light information emitted by the diagnosis sample S. FIG.

The test unit 430 may be formed in plural numbers according to the type of the diagnostic sample S, and the test material may be changed according to the type and the target material of the diagnostic sample S. FIG.

The reference unit 440 is formed at a rear side with respect to the moving direction of the diagnostic sample S from the third region of the flow channel 400, that is, the second region of the flow channel 400.

The reference unit 440 is formed with a reference material for confirming a reference level of the diagnostic sample S that has been tested for diagnosing a disease in the third region, and the diagnostic sample (440) passed through the reference unit 440. By irradiating light to S), it is possible to check the reference level of the diagnostic sample S according to the light information I emitted by the diagnostic sample S. FIG.

The reference unit 440 may be formed in plural numbers according to the type of the diagnostic sample S, and the reference material may be changed according to the type of the diagnostic sample S and the target material.

That is, the material formed in the sample buffer unit 410, the preprocessor 420, the test unit 430, and the reference unit 440, the degree of reaction with the diagnostic sample S, and the diagnostic sample S According to the state of the, the diagnostic sample (S) generates different optical information (I) by the light irradiated to the diagnostic sample (S), and performs the diagnosis of the disease through the detected optical information (I). .

The bonding layer 300 is formed between the upper plate 100 and the lower plate 200 to combine the upper plate 100 and the lower plate 200, the color of the bonding layer 300 Is composed of black.

The color of the bonding layer 300 may be formed by including a dye such as a liquid dye or a nano pigment in the bonding layer 300.

Alternatively, the bonding layer 300 may be made black by using the bonding agent itself constituting the bonding layer 300 as black.

At this time, the upper plate 100 and the lower plate through a separate non-electric (not shown) to determine whether the bonding layer 300 formed between the upper plate 100 and the lower plate 200 is defective. The bonding state of the 200 may also be checked.

As shown in FIG. 3, the light irradiated to the diagnostic sample S to detect the optical information I of the diagnostic sample S is irradiated not only to the diagnostic sample S but also to the bonding layer 300. In this case, as the bonding layer 300 is formed in black, the diffuse reflection phenomenon R generated from the bonding layer 300 may be minimized to reduce the BG value, which will be described later.

In addition, the diagnostic chip for precise detection of the diagnostic sample optical information according to the present invention may further include a sticker layer (not shown).

The sticker layer may be disposed between the upper plate 100 and the lower plate 200 or on the lower surface of the lower plate 200, and the color of the sticker layer is black.

The sticker layer is black and disposed between the upper plate 100 and the lower plate 200 or on the lower surface of the lower plate 200, thereby minimizing diffuse reflection R generated from the bonding layer 300. To help reduce the BG value.

The BG value according to the color of the bonding layer 300 will be described with reference to FIGS. 3 to 6 as follows.

First, the color of the bonding layer 300 is blue, red, orange, orange, yellow, green, violet, pink, pink, brown, brown When the color is black or colorless, the BG value due to the diffuse reflection R generated in the bonding layer 300 is as shown in FIG. 4.

As shown in FIG. 4, the bonding layer 300 is configured with the above-described color, and the BG value due to the diffuse reflection R generated from the bonding layer 300 when the light is irradiated along the diagnostic chip is When the bonding layer 300 is blue it can be confirmed that the highest.

At this time, the higher the BG value by the bonding layer 300, the greater the noise when detecting the optical information (I) from the diagnostic sample (S).

This means that when information other than the optical information I is involved when diagnosing the presence or absence of a disease that the diagnostic sample S has through the optical information I detected from the diagnostic sample S, This is because interference with the optical information I may occur.

As shown in FIG. 5, when the color of the bonding layer 300 is composed of red, orange, yellow, green, purple, pink, brown, black, and colorless except for blue, occurs in the bonding layer 300. FIG. 6 is a graph illustrating BG values due to diffuse reflection R, and FIG. 6 is a graph in which BG values for colors of the bonding layer 300 are adjusted based on colorlessness in FIG. 5.

As shown in FIG. 5 and FIG. 6, when the color of the bonding layer 300 is black, the BG value is the lowest. Accordingly, the BG value is minimized by minimizing the diffuse reflection phenomenon (R) occurring in the bonding layer 300. Since it can be reduced, it is possible to more accurately detect the optical information (I) of the diagnostic sample (S).

Therefore, in order to minimize interference of the optical information (I) detection, it is preferable to configure the bonding layer 300 in black having the lowest BG value.

As described above, the present invention is not limited to the above-described specific preferred embodiments, and various changes and modifications may be made by those skilled in the art without departing from the scope of the present invention as claimed in the claims. And such variations are within the scope of the present invention.

100: upper plate
110: diagnostic sample inlet
120: diagnostic sample movement hole
200: lower plate
300: bonding layer
400: flow channel
410: sample buffer unit
420: preprocessor
430: test unit
440: reference unit

Claims (3)

An upper plate having a flow channel through which the diagnostic sample is moved;
A lower plate coupled to a lower portion of the upper plate; And
And a bonding layer formed between the upper plate and the lower plate to bond the upper plate and the lower plate.
The upper plate,
A diagnostic sample inlet connected to one end of the flow channel to which the diagnostic sample is input; And
And a diagnostic sample movement control hole connected to the other end of the flow channel to control movement of the diagnostic sample.
Diagnostic chip for precise optical information detection of the diagnostic sample, characterized in that the color of the bonding layer is black. The method of claim 1,
The color of the bonding layer is a diagnostic chip for precise detection of optical information of the diagnostic sample, characterized in that the bonding layer is formed by containing a liquid dye or a nano pigment. The method of claim 1,
Further comprising a sticker layer disposed between the upper plate and the lower plate or on the lower surface of the lower plate,
The diagnostic chip for the optical information precision detection of the diagnostic sample, characterized in that the color of the sticker layer is black.

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