KR20150020804A - Light-permeable diagnostic kit and reader having the same - Google Patents

Abstract

The present invention relates to a permeable diagnostic kit and a reader with the same. The permeable diagnostic kit comprises: a diagnostic strip where a sample pad and a signal detecting pad are included on one surface of a supporting body; and a diagnostic kit case which accommodates the diagnostic strip, and has windows corresponding to the signal detecting pad at both sides thereof. The supporting body, or part of or the entire supporting body area corresponding to the signal detecting pad is transparent. Therefore, the permeable diagnostic kit can quantitatively calculate even the amount of a target material with a low concentration only by measuring permeability according to the concentration of the target material without the need to focus like the conventional image sensor.

Description

A transmission type diagnostic kit and a reader including the same,

The present invention relates to a disease diagnostic apparatus widely used for in vitro diagnosis, and more particularly, to a LFA (lateral flow assay) type diagnostic strip. More specifically, The present invention relates to a transmission type diagnostic kit and a reader including the same.

An apparatus for examining or examining the presence of a single or multiple substances in a liquid sample, for example a urine or blood sample, is referred to as a diagnostic kit. Specifically, the modern diagnostic business field is integrated into one of the Point-Of-Care Testing (POCT). POCT refers to equipment that can be used by a general public who does not have expertise in a centralized laboratory. Currently, diagnostic fields are expanding from hospitals to field and individual.

In particular, the rapid diagnostic kits typified by immunochromatographic analysis are used to identify diseases or detect changes in the healthcare field, and they can be used for qualitative and quantitative analysis of trace analytes in a variety of fields, It is being developed in a simple way. In the field of health care, applications are being extended to pregnancy, ovulation, infectious diseases, drug abuse, acute myocardial infarction, and cancer.

1 is a perspective view showing the structure of a LFA (lateral flow assay) type diagnostic strip using an immunochromatographic method. 1, a general immunochromatography diagnostic strip used for immunochromatographic analysis comprises a support 10 in the form of an elongated rectangle made of an adhesive plastic material, The conjugate pad 40, the signal detection pad 30, and the absorption pad 50, which are arranged substantially in sequence from the sample pad 20 side.

In this way, in the LFA diagnostic strip using the conventional immunochromatographic method, the detection antibody polymerized with the labeling substance is accumulated in advance in the dry state on the conjugation pad 40, and the sample passes through the sample pad 20, (40), the conjugate dissolves and reacts with the analyte in a liquid phase. This reaction takes place when the sample solution moves under fluid flow caused by the capillary phenomenon, and the sandwich bond forms an immunoconjugate between the conjugate and the analyte (antigen). The immunoconjugate moves to the signal detection pad 30 on the membrane and is captured by the immobilized antibody that specifically reacts with the analyte to cause a reaction proportional to the analyte concentration. The color of the detection region 31 existing in the signal detection pad 30 is changed according to the degree of the reaction, and the user makes a diagnosis by confirming the change of the color thus changed. The change of the color can be visually recognized or the magnitude of the fluorescence signal generated in proportion to the degree of the reaction can be measured by a camera or the like. The analyte that has passed through the detection region 31 can be confirmed to have undergone a normal lateral flow assay by way of the control region 32 and the analyte can be detected on the absorption pad 50 Absorbed.

FIG. 2 is a cross-sectional view and a partially enlarged view showing the structure of a conventional general LFA type diagnostic strip. As shown in FIG. 2, the conventional signal detection pad 30 has a support plate 33 at the lower end thereof And the support 10 is bonded thereto. There has also been an attempt to further enhance the color change in the detection area 31 of the signal detection pad 30 by adding white paper 11 to the back of the support 10.

However, these conventional methods have an advantage of being able to confirm the test results simply, but it is difficult to obtain the detection result when the concentration or the amount of the analyte is small, and the degree of subtle color change is accurately and consistently It is difficult to confirm it, so it is used only for qualitative purposes. In other words, it is difficult to quantitatively analyze the concentration or amount of the analyte in an absolute amount, and thus it can not be utilized for purposes other than a simple qualitative test such as a diagnosis of pregnancy.

Furthermore, there has also appeared a technique of acquiring image information for the detection region 31 using an image sensor that photographs the detection region 31, and converting the digital image signal into a digital signal for quantitative analysis. However, this technique requires the use of an image sensor in order to acquire image information. It is difficult to focus the image sensor, the optical system configuration for processing image information becomes large, and quantitative analysis The algorithm is complicated and the reliability is low.

In addition, an attempt has been made to implement a reader system for quantitative analysis by an optical method for measuring fluorescence using a fluorescent substance as a labeling material. However, the problem is that the configuration of the fluorescence measuring optical system is difficult and expensive, and the cost of the fluorescent labeling material is high have.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a transmission type diagnostic kit which does not need to focus on a conventional image sensor.

The present invention is intended to quantitatively calculate the amount of a target substance at a low concentration by simply measuring the permeability according to the concentration of the target substance.

In addition, the present invention aims to obtain the accurate diagnosis result more easily by continuously scanning the detection area and the verification area of the diagnostic strip.

According to an aspect of the present invention, there is provided a transmission type diagnostic kit comprising: a diagnostic strip having a sample pad and a signal detection pad on one surface of a support; And a diagnostic kit case which houses the diagnostic strip and has windows corresponding to the signal detection pads on both sides, wherein all or part of the support region corresponding to the support or the signal detection pad is transparent.

Here, the support is preferably a transparent substrate.

The signal detection pad may be entirely or partially transparent.

In addition, the signal detection pad may be formed of a nitrocellulose membrane.

Preferably, the signal detection pad includes a detection area and a verification area, and the window is smaller than the area of the signal detection pad and larger than the area including the detection area and the verification area.

According to another aspect of the present invention, there is provided a transmission type diagnostic kit comprising: A linear light source positioned at one side of the transmission type diagnostic kit; And a photodetector positioned on the other side of the transmission type diagnostic kit.

Here, the light source is preferably a laser.

Further, the present invention may further comprise a collimator positioned between the transmission type diagnostic kit and the light source, and the collimator may have a slit having a size corresponding to the detection area or the verification area of the signal detection pad Do.

The collimator may have a slit having a smaller size than the detection area or the verification area of the signal detection pad.

It is also preferable that the present invention further comprises a moving means for moving the transmission type diagnostic kit in a horizontal direction.

In addition, the moving means can move the transmission type diagnostic kit one or more times by reciprocating.

In addition, the moving means may move the transmission type diagnostic kit by the window length of the transmission type diagnostic kit.

Preferably, the photodetector includes a processing unit for calculating a transmittance through which the light emitted from the light source passes through the detection region of the transmission type diagnostic kit.

The details of other embodiments are included in the detailed description and drawings.

The present invention can produce a transmission type diagnostic kit characterized in that all or part of the support of the diagnostic strip is transparent and a window is provided on both sides of the case accommodating the diagnostic strip.

Such a transmission type diagnostic kit can quantitatively calculate the amount of a target substance at a low concentration only by measuring the transmittance according to the concentration of the target substance without having to focus on the same as with the conventional image sensor.

In addition, the transmission type diagnostic kit reader according to the present invention is characterized by further comprising moving means for moving the transmission type diagnostic kit in the horizontal direction. By continuously scanning the detection region and the control region of the diagnostic strip, It can be obtained more easily.

1 is a perspective view showing the structure of a conventional LFA (lateral flow assay) type diagnostic strip,
2 is a cross-sectional view and a partially enlarged view showing the structure of a conventional general LFA type diagnostic strip,
FIG. 3 is a cross-sectional view showing the structure of a transmission type diagnostic kit according to a preferred embodiment of the present invention,
FIG. 4 is a cross-sectional view showing an example in which a support in a transmission type diagnostic kit according to the present invention includes a transparent support and an opaque support,
5 is a cross-sectional view for explaining an example of the lengths of the transparent support portion and the opaque support portion of the support in the transmission type diagnostic kit according to the present invention,
6 is a cross-sectional view and a partially enlarged view showing an example of a cross-sectional configuration of a diagnostic strip in a transmission type diagnostic kit according to the present invention,
FIG. 7 is a cross-sectional view and a partially enlarged view showing an example in which a transparent plate exists between a support of a diagnostic strip and a signal detection pad in a transmission type diagnostic kit according to the present invention,
FIG. 8 is a schematic diagram showing a configuration of a transmission type diagnostic kit reader according to another preferred embodiment of the present invention,
FIG. 9 is a photograph showing an example in which the detection regions of the transmission type diagnostic kit according to the present invention have reactive substance concentrations,
10 is a graph showing an example in which different transmittances are measured according to the concentration of a reactant from the detection region of FIG.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.

3 is a cross-sectional view showing a structure of a transmission type diagnostic kit 1 according to a preferred embodiment of the present invention.

The diagnostic kit 1 according to the present invention shown here comprises a diagnostic strip 100 and a diagnostic kit case 200 accommodating the same. The diagnostic kit 1 according to the present invention can be a disease diagnostic device that can be used for in vitro diagnosis, and can be a detector of an analyte, an immunochromatographic analyzer or an immunity reaction tester. Preferably, the analyte is LFA lateral flow assay type strips or cartridges.

The diagnostic strip 100 is basically provided with a sample pad 120 and a signal detection pad 130 on one surface of a support 110. That is, the diagnostic strip 100 includes all common structures used in immunochromatographic analysis. The diagnostic strip 100 includes an elongated rectangular shaped support 110 made of an adhesive plastic material and a sample pad 120, a conjugate pad 140, A signal detection pad 150, and an absorption pad 150. [ The sample pad 120 absorbs the liquid sample (or analytical sample) to be analyzed and ensures uniform flow of the liquid sample. The conjugate pad 140, which is partially overlapped with the other end of the sample pad 120, includes a flowable conjugate that specifically binds the analyte contained in the liquid sample, 120 passes through the conjugate pad 140 in the X direction, resulting in a specific binding between the analyte and the flowable conjugate. The sample pad 120 and the signal detection pad 130 disposed at a position subsequent to the conjugate pad 140 are provided with a detection zone 131 and a contrast zone control zone 132. [0034] FIG. At this time, the detection region 131 is an area for confirming whether or not an analyte exists in the liquid sample, and the verification region 132 is an area for checking whether or not the liquid sample has normally passed through the detection region 131 . In the present invention, the signal detection pad 130 may be a reaction detection unit in which an immune reaction occurs when a sample is developed. The reaction detection unit includes a detection region 131 and a non-reaction region 132, It is possible to further include a background area as a background area. The absorbing pad 150 may be disposed at a position subsequent to the signal detecting pad 130, that is, at a position adjacent to the other end of the supporting body 110. [ This absorbent pad 150 absorbs the liquid sample that has passed through the signal detection pad 130 and helps capillary flow of the liquid sample on the immunochromatographic diagnostic strip 100.

The diagnostic kit case 200 accommodates the above-described diagnostic strip 100, and its shape and size are not particularly limited and include all types known in the art. A sample injection port 230 may be provided on one side of the diagnostic kit case 200 and the upper case 210 and the lower case 220 may be separated from each other.

In the diagnostic kit 1 having the structure as described above, the entirety or a part of the support 110 is transparent and the diagnostic kit case 200 is positioned at a position corresponding to the signal detection pad 130 And has windows 211 and 221 on both sides thereof. As an example, the present invention may be such that the entire support 110 is transparent (see FIG. 3).

In addition, the diagnostic kit case 200 has windows 211 and 221 made of open windows or transparent windows on both sides thereof. That is, the upper case 210 has an upper window 211 and the lower case 220 has a lower window 221 corresponding to the upper window 211. It is preferable that the windows 211 and 221 are formed at positions facing each other corresponding to the signal detection pad 130. The diagnostic kit case 200 is intended to transmit light from one side of the diagnostic strip 100 to the other side and the windows 211 and 221 are formed on all or part of the cases 210 and 220 And the diagnostic kit case 200 itself may be transparent.

Since the conventional support 10 is made of an opaque material as shown in FIGS. 1 and 2, and the case for accommodating the conventional support 10 has a window only on one side thereof, the conventional support 10 There was no need to be transparent.

However, the support 110 according to the present invention is characterized in that all or a part of the support 110 is made transparent as described above, through which the light can pass through the diagnostic strip 100. In addition, the diagnostic kit case 200 according to the present invention also has windows 211 and 221 on both sides thereof, through which the light can pass through the diagnostic kit case 200. Therefore, since the portion corresponding to at least the signal detection pad 130 is transparent on the support 110 and the diagnostic kit case 200 corresponding thereto also has the windows 211 and 221, The light of the light source which has flowed into one side of the diagnostic kit 1 can be transmitted to the other side through the diagnostic kit 1. That is, the light emitted from the light source can pass through the signal detection pad 130 including the detection region 131, and in this process, the amount of light transmitted through the detection region 131 And the amount of the analyte can be quantitatively analyzed by detecting the amount of the light.

The present invention is characterized in that all or a part of the support 110 of the diagnostic strip 100 is transparent and windows 211 and 221 are provided on both sides of the case 200 accommodating the diagnostic strip 100. [ The diagnostic kit 1 can be provided. Such a transmission type diagnostic kit 1 has the effect of quantitatively calculating the amount of analyte at a low concentration by measuring the transmittance according to the concentration of the analyte without the need to focus on the same as with the conventional image sensor.

It will also be apparent to those skilled in the art that diagnostic kits of the type comprising only the diagnostic strip 100 according to the present invention and not including the diagnostic kit case 200 may also be included in the present invention.

4 is a cross-sectional view showing an example in which the support 110 in the transmission type diagnostic kit 1 according to the present invention includes the transparent support portion 111 and the opaque support portion 112, and FIG. 5 is a cross- Sectional view for explaining an example of the length of the transparent supporting part 111 and the opaque supporting part 112 of the supporting body 110 in the transmission type diagnostic kit 1. FIG.

Another feature of the present invention is that the entire area of the support 110 corresponding to the signal detection pad 130 is transparent or the support 110 corresponding to the signal detection pad 130 ) Region is transparent (see Fig. 5). Here, the region of the support 110 corresponding to the signal detection pad 130 means a position where the signal detection pad 130 is mounted on the support 110. That is, the portion of the support 110 which contacts the signal detection pad 130 is composed of the transparent support portion 111 having the same or similar size as the signal detection pad 130.

The support 110 or a part thereof may be made of a transparent material such as glass, transparent polymer or transparent plastic, and when the entire support 110 is made of a transparent substrate, it is made of a single material, . Further, when a part of the support 110 is transparent, there is an advantage that the light is not diffused or refracted to a part of the opaque part and the straightness is excellent. That is, when the support 110 includes the transparent support 111 having a size corresponding to the signal detection pad 130, the light is not diffused or refracted toward the opaque supporter 112, .

The signal detection pad 130 may include a detection area 131 and a verification area 132 as described above so that the windows 211 and 221 are spaced apart from the area of the signal detection pad 130 (D2), which is smaller than the area D1 and is larger than the area surrounded by the detection area 131 and the verification area 132 (see FIGS. 4 and 5). That is, both the detection area 131 and the signal detection pad 130 that does not include the verification area 132 are limited by the sizes of the windows 211 and 221. It is preferable that light passing through the kit 1 according to the present invention is transmitted through a minimum area, and it is more preferable to transmit the light through at least the detection area 131 and the check area 132. As described above, when the lengths of the windows 211 and 221 are minimized while including at least the detection region 131 and the verification region 132, when moving the diagnostic kit 1 according to the present invention as described below, The distance can be reduced. In addition, by placing the upper case 210 or the lower case 220 instead of the windows 211 and 221 as close as possible to the vicinity of the detection region 131 and the verification region 132, And it is easy to confirm the result.

The windows 211 and 221 are provided to support the edge of the support 110 so that the support 110 can be physically supported by the diagnostic kit case 200 and the detection areas 131 And the reference area 132. In this case,

6 is a cross-sectional view and a partial enlarged view showing an example of a sectional configuration of the diagnostic strip 100 in the transmission type diagnostic kit 1 according to the present invention. Sectional view and a partial enlarged view showing an example of a transparent plate 133 being present between the support 110 and the signal detection pad 130 of the liquid crystal display device 100 shown in FIG.

The signal detection pad 130 of the diagnostic strip 100 according to the present invention may be in contact with the support 110 and the signal detection pad 130 and the support 110 may be exposed to light It is preferable that it is made of a material that can permeate. That is, the signal detection pad 130 may be entirely or partially transparent. The signal detection pad 130 may be made of a variety of transparent materials known in the art. Among them, the nitrocellulose membrane is more preferable because it has strong affinity for nucleic acids and proteins .

The signal detection pad 130 and the support 110 may be fixed to each other by a separate adhesive or a pressure sensitive adhesive The signal detection pad 130 has a function of supporting the signal detection pad 130 more firmly.

FIG. 8 is a schematic diagram showing a configuration of a transmission type diagnostic kit reader according to another preferred embodiment of the present invention.

Another embodiment of the present invention shown here includes the above-described transmission type diagnostic kit; A linear light source 300 positioned at one side of the transmission type diagnostic kit; And a photodetector (400) located on the other side of the transmission type diagnostic kit. In FIG. 8, a diagnostic strip 100 or a signal detection pad included therein is shown instead of the diagnostic kit 1 according to the present invention.

The light source 300 includes various light sources known in the art and is preferably a transmissive linear light source, for example, a linear laser light source may be used. When the label material is gold nanoparticles in the detection region 131, the gold nanoparticles are visually red. Therefore, in order to improve the measurement sensitivity, a green light source (absorption wavelength of gold nanoparticles) .

The photodetector 400 corresponds to the light source 300 and detects light emitted from the light source 300. The photodetector 400 may be a PD (photo detector), although not limited thereto.

The light source 300 and the photodetector 400 are positioned so as to face each other with respect to the diagnostic kit (or the diagnostic strip 100) according to the present invention, To reach the photodetector (400).

Since the transmission type diagnostic kit reader according to the present invention can quantitatively analyze analytes by the transmittance of light transmitted through the diagnostic kit according to the present invention, there is no need to focus on the image sensor as in the conventional image sensor, The composition can be minimized, and the analyte can be quantitatively analyzed easily and easily by the permeability.

In addition, the diagnostic kit reader according to the present invention may further include a diffuser 500 for uniformly distributing light between the light source 300 and the diagnostic kit.

In addition, it is preferable to make the spot size of the light source smaller than the detection area by using a lens or a slit because the light transmission characteristic of a local part of the detection area can be accurately detected.

The diagnostic kit reader according to the present invention further includes a collimator 600 positioned between the transmission type diagnostic kit (or the diagnostic strip 100) and the light source 300, It is possible to have a slit 610 of a size corresponding to the detection region 131 and / That is, the slit 610 passes through the slit 610 so that the light reflected on the detection area 131 and / or the verification area 132 is the same size as the detection area 131 and / As shown in FIG. Since the transmittance in the detection region 131 and the check region 132 is important for diagnosis of the analyte, the transmitted light can be concentrated on this portion.

It is further preferable that the collimator 600 has a slit having a smaller size than the detection area 131 and / or the verification area 132 of the signal detection pad 130. If the light transmitted through the detection area 131 and / or the verification area 132 is larger than the detection area 131 and / or the verification area 132, the detection area 131 and / (For example, increase in transmittance) may occur in the transmittance in the region 132, which is not preferable.

In addition, the diagnostic kit reader according to the present invention may further include a moving unit 700 for moving the transmission type diagnostic kit (or the diagnostic strip 100) in the horizontal direction. The moving means 700 includes all the various means known in the art and can be, for example, a scanner, with or without a diagnostic kit according to the present invention. The horizontal direction refers to the longitudinal direction of the diagnostic kit (or diagnostic strip 100) according to the present invention, the direction in which the analyte flows, the direction from the detection region 131 to the contrast region 132, ), A direction perpendicular to the light emitted therefrom, or a similar direction.

According to the moving means 700, the transmission type diagnostic kit (or the diagnostic strip 100) according to the present invention can be moved in the direction perpendicular to the light source 300 or the light emitted therefrom, It is possible to transmit light successively and / or in the reverse order to the detection region 131 and the verification region 132 in a sequential and / or reverse order, I have. It is also possible to comparatively analyze the transmittances of the detection region 131 and the verification region 132 using the transmittance information on the background region between the detection region 131 and the verification region 132.

It is preferable that the moving means 700 move the transmission type diagnostic kit 1 by the length of the windows 211 and 221 of the transmission type diagnostic kit 1. Since the detection area 131 and the verification area 132 are both included in the length of the windows 211 and 221 and the area of the windows 211 and 221 that is outside the length of the windows 211 and 221 is blocked by the case 200, It is possible to detect a change in the transmittance of a large width by the case 200, and it is easy to confirm the result if the window 200 is moved only by the same or similar length as the length of the windows 211 and 221.

The moving unit 700 can move the transmission type diagnostic kit 1 more than once by reciprocating. In this case, it is possible to obtain two or more transmissions of the detection area 131 and the verification area 132 And it is preferable to average them to obtain more accurate results with no error.

The diagnostic kit reader or the photodetector 400 according to the present invention may be configured such that the light emitted from the light source 300 passes through the detection region 131 and / or the verification region 132 of the transmission type diagnostic kit Or may include a processing unit 800 that compares the transmittance passing through the detection region and the background region (non-reaction region).

FIG. 9 is a photograph showing an example in which the detection regions of the transmission type diagnostic kit according to the present invention have mutual reactant concentrations. FIG. 10 is a graph Fig.

As shown in Fig. 9, diagnostic strips each containing three analytes having different concentrations were prepared. That is, a stock solution (# 2), a 1/10 dilution solution of the stock solution (# 3) and a 1/50 dilution solution (# 5) of the stock solution were prepared. Using the diagnostic kit reader according to the present invention, The results are shown in FIG. 10.

As shown in FIG. 10, it was found that the output characteristics (transmittance) of the PD were different according to the concentration of the analyte, and it was confirmed that the accurate concentration thereof can be quantitatively calculated up to 1/50 dilution of the stock solution.

Although the present invention has been shown and described with respect to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the following claims It will be apparent to those skilled in the art.

1: Diagnostic Kit
100: diagnostic strip
110: Support
111: transparent support
112: opaque support
120: Sample pad
130: Signal detection pad
131: detection area
132:
133: transparent plate
140: Conjugation pad
150: Absorption pad
200: Case
210: upper case
211: upper window
220: Lower case
221: Lower window
230: inlet
240:
300: Light source
400: detector
500: diffuser
600: collimator
610: slit
700: Moving means
800:

Claims (13)

A diagnostic strip having a sample pad and a signal detection pad on one side of a support; And
And a diagnostic kit case accommodating the diagnostic strip and having windows corresponding to the signal detection pads on both sides,
Wherein all or a part of the support region corresponding to the support or the signal detection pad is transparent.
The method according to claim 1,
Wherein the support is a transparent substrate.
The method according to claim 1,
Wherein the signal detection pad is entirely or partially transparent.
The method according to claim 1,
Wherein the signal detection pad is made of a nitrocellulose membrane.
The method according to claim 1,
Wherein the signal detection pad includes a detection area and a verification area,
Wherein the window is smaller than the area of the signal detection pad and larger than the area including the detection area and the verification area.
A transmission type diagnostic kit according to any one of claims 1 to 5;
A linear light source positioned at one side of the transmission type diagnostic kit; And
And a photodetector positioned on the other side of the transmission type diagnostic kit.
The method according to claim 6,
Wherein the light source is a laser.
The method according to claim 6,
Further comprising a collimator positioned between the transmission type diagnostic kit and the light source,
Wherein the collimator has a slit having a size corresponding to a detection area or a verification area of the signal detection pad.
9. The method of claim 8,
Wherein the collimator has a slit smaller in size than the detection area or the verification area of the signal detection pad.
The method according to claim 6,
Further comprising moving means for moving the transmission type diagnostic kit in a horizontal direction.
11. The method of claim 10,
Wherein the moving means reciprocates the transmission type diagnostic kit one or more times.
11. The method of claim 10,
Wherein the moving means moves the transmission type diagnostic kit by a window length of the transmission type diagnostic kit.
The method according to claim 6,
Wherein the photodetector includes a processor for calculating a transmittance of light emitted from the light source through the detection region of the transmission type diagnostic kit.

Images