KR20190086092A - Medicinal herbs composition for enhancing immune activity - Google Patents

Abstract

The present invention relates to a medicinal herb composition for enhancing immune activity, containing Hericium erinaceus, Sparassis crispa, red ginseng, Wolfiporia extensa, Asparagus cochinchinensis, Liriope muscari, Lycium chinense, Stachys sieboldii, Rehmannia glutinosa, and honey. The medicinal herb composition for enhancing immune activity of the present invention has effects of reducing tumor necrosis factor α (TNF-α), IL-2, and IL-6, which are inflammatory cytokines, and also reducing the immunoglobulin E (IgE). In addition, the medicinal herb composition has an effect of increasing white blood cells (WBC), neutrophils, lymphocytes, and monocytes.

Description

[0001] Medicinal herbs composition for enhancing immune activity [

The present invention relates to a herbal medicinal composition for enhancing immunity activity, and more particularly, to a herbal medicinal composition for enhancing immunity activity, which comprises a medicinal herb composition for enhancing immune activity, .

As interest in health increases, interest in foods, preparations, and treatments that can improve immunity is also increasing.

Gyeongokgyo (膏) is one of the prescriptions of oriental medicine, ginseng, Baekbokryeong, raw bamboo, is composed of medicinal herbs. It is said that Kiyokoko fills the jung, sees the soul, darkens the hair, exposes the teeth, and mankind removes the white disease. It is the sucker suyunjin ji (益 寿 永 眞 膏) which puts in the gyungokgyo more than the quanmundong, the moonmundong, and the gugija. In addition to the materials that can be added to the Kwangyang High School, it is a form of enhancing the effect of enhancing the quality of the ginseng (益气 陰阴). (Kim MD, The literature study on the efficacy and manufacturing process of Gyeongoggo, J Oriental Medical Classics, 2011; 24 (2): 51-64). Gyeongokgokgo and the sucker is a representative form of gyeonggye (膏) to see that the (补).

Techniques are being developed to add functional ingredients or other medicinal materials to enhance the effectiveness of the Kyohei Kyo or the Suyu Yeonjin. Korean Patent No. 10-1198211 (Nov. 7, 2012) discloses a method for producing ginseng ginseng using sea cucumber. In the mixture of ginseng extract, red ginseng powder and white ginseng powder, Dried, and pulverized to obtain a mushroom. Korean Patent No. 10-1016195 (Feb. 23, 2011) discloses a ginseng and bokbok-gyeong, which is prepared by grinding ginseng juice, extracting ginseng juice, infusing ginseng powder and Baekbokryeong powder into the above- Which is prepared by adding the above-mentioned gumma to the process of preparing a saccharified liquid from glutinous rice by storing, boiling, cooling and boiling.

Accordingly, the inventors of the present invention have found that a herbal medicine composition comprising a herbal composition comprising mulberry, mushroom, and mugwort as an additional ingredient in a composition comprising a subsoil Youngjin (red ginseng, Baekbokgyeong, And reached the present invention.

Korean Registered Patent No. 10-1198211 (Nov. 07, 2012) Korean Patent No. 10-1016195 (Feb. 22, 2011)

It is an object of the present invention to provide a herbal medicine composition for enhancing immunity.

Another object of the present invention is to provide a method for preparing a herbal medicine for enhancing immunity.

To achieve the above object, the present invention provides a medicinal herbal composition composition for enhancing immunity activity, comprising a mushroom, a mushroom, a red mushroom, a red mushroom, a ginseng, a ginseng, a ginseng, a ginseng,

In one embodiment of the present invention, the bokryeong may be Baekbokgolryeong, Bokryeoppi, or a mixture thereof.

In one embodiment of the present invention, the fresh persimilis may be a fresh persimmon juice, guar gum, or a mixture thereof.

In one embodiment of the present invention, the herbal medicinal composition for immunostimulatory enhancement comprises 1 to 7% by weight of Herring mushroom, 0.5 to 5% by weight of mushroom, 2 to 10% by weight of red ginseng, 5 to 15% 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of base stone, 30 to 53% .

In one embodiment of the present invention, the herbal medicine composition for immunostimulatory enhancement may be fermented with a strain of Leuconostoc mesenteroides subsp. Dextranicum .

In one embodiment of the present invention, the fermentation may be carried out at 33 to 38 DEG C for 3 to 7 days.

In one embodiment of the present invention, the herbal medicinal composition for enhancing immunity may be used in cosmetics, health supplements or pharmaceutical compositions.

In one embodiment of the present invention, the herbal medicinal composition for enhancing immunity may be prepared in the form of a soft-acting preparation, a ring, a tablet or a gum.

In order to accomplish another object of the present invention, the present invention provides a method for preparing a herbal medicine for immunoactivity enhancement using a method for preparing a herbal medicine composition for enhancing immunity.

The herbal medicinal composition for immunoactivity enhancement of the present invention reduces TNF-α, IL-2 and IL-6, which are inflammatory cytokines, and inhibits the immune globulin E (Immunoglobulin E, IgE) (WBC), neutrophils, lymphocytes, and monocytes, as well as reducing the number of white blood cells (WBCs).

In addition, the herbal medicinal composition for enhancing immunity activity of the present invention is useful as a herbicide for Leuconostoc mesenteroides subsp. Dextranicum , which is excellent in acid resistance and resistance to corrosion and has antibacterial activity against harmful bacteria such as Escherichia coli . The bacteria can survive to enter the stomach through the gastric and digestive tracts to inhibit harmful microorganisms in the intestines and prevent infection with pathogenic microorganisms. Thus, . ≪ / RTI >

The herbal medicine composition for enhancing immunity of the present invention can be used in cosmetics, health supplements or pharmaceutical compositions.

The herbal medicinal composition for immunoactivity enhancement of the present invention can reduce cost and reduce environmental pollution by reducing garbage generation by utilizing costly mushroom basin, bamboo mushroom, or persimmon leaf which was previously disposed of.

1 is a phylogenetic analysis chart of 16S rRNA sequence analysis of Leuconostoc mesenteroides subsp. Dextranicum strain finally selected in the present invention.
FIG. 2 is a graph showing the effect of the immunomodulating composition of the present invention on the TNF-.alpha. Content in rats.
FIG. 3 is a graph showing the effect of the immunomodulating composition of the present invention on IL-2 content in rats.
FIG. 4 is a graph showing the effect of the immunomodulating composition of the present invention on IL-6 content in rats.
FIG. 5 is a graph showing the effect of the immunomodulating composition of the present invention on the serum IgE content in rats.
6 is a graph showing the effect of the immunomodulating composition of the present invention on the spleen weight of a mouse.
7 is a graph showing the effect of the immunomodulating composition of the present invention on leukocytes in the blood of rats.
FIG. 8 is a graph showing the effect of the immunomodulating composition of the present invention on erythrocytes in the blood of rats.
FIG. 9 is a graph showing the effect of the immunomodulating composition of the present invention on the weight gain of rats.
10 is a graph showing the effect of the immunomodulating composition of the present invention on the serum aminotransferase content in rats.

The herbal medicine composition for enhancing immunity activity of the present invention is characterized by comprising a pine mushroom, a mushroom, a red mushroom, a red mushroom, a red mushroom, a red mushroom, a bongryeong, a milky way,

In the present invention, powdery forms of Myrrhore mushroom, Myrrh mushroom, Red ginseng, Byeongryong, Chunmun-dong, Maekmundong, Gejiae and Corundum are preferably used.

In the present invention, the herbal medicine composition for immunostimulatory enhancement is characterized in that it comprises 1 to 7% by weight of Herring mushroom, 0.5 to 5% by weight of mushroom, 2 to 10% by weight of red ginseng, 5 to 15% 0.5 to 5% by weight of gypsum, 0.5 to 5% by weight of gypsum, 0.5 to 5% by weight of gypsum, 0.5 to 5% by weight of a cornerstone, 30 to 53% by weight of raw persimmon and 25 to 40% by weight of gypsum.

In the present invention, the mushroom may be used alone or in combination with a fruiting body or a stipe of mushroom.

The above-mentioned Zygomy mushroom basal part is obtained through a process of separating from fruiting body in harvested Zygomy mushroom. When the mushroom is harvested by the sawdust cultivation method, it is preferable that the base separated from the fruiting body is subjected to a pretreatment for removing sawdust by a dry mill.

In the present invention, the bokyong can be Baekbokgulryeong, Boryeonghyeok, or a mixture thereof. When Baekbokwolryeong is mixed and used, it is preferable that Baekbokryeong 3 to 13% by weight and Baeryeongpyipi 0.5 to 5% by weight based on the total weight of the composition.

In the present invention, the raw raw material can be used as raw juice, jujube or a mixture thereof. It is the raw juice of the raw persimmon which presses the fresh persimilis and exits in the liquid form, and it is the persimmon persimmon which remains as the residue by pressing. When the raw juice is mixed with rhubarb, it is preferable that the raw juice is contained in an amount of 25 to 40% by weight and 10 to 25% by weight in terms of the total weight of the composition.

In the present invention, the herbal medicine composition for immunostimulatory enhancement is characterized in that it comprises 1 to 7% by weight of Herring mushroom, 0.5 to 5% by weight of mushroom, 2 to 10% by weight of red ginseng, 3 to 13% 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of starch, 0.5 to 5% by weight of base stone, 25 to 40% by weight of raw juice, 10 to 25% And 25 to 40% by weight of corn syrup.

On the other hand, the herbal medicine composition for immunoactivity enhancement of the present invention can be fermented into a strain of Leuconostoc mesenteroides subsp. Dextranicum .

In the present invention, the fermentation is preferably carried out at 33 to 38 DEG C for 3 to 7 days, more preferably at 36 to 37 DEG C for 4 to 6 days.

The herbal medicinal composition for enhancing immunity activity of the present invention can be manufactured in the form of a soft-drink preparation, a ring, a pill or a gum.

In particular, the herbal medicinal composition for enhancing immunity activity of the present invention can be prepared into a high-dose formulation using a double boiling method.

Bokyeong is a kind of mushroom and mushroom. It is parasitic to tree roots such as pine trees. The surface of the mushrooms is reddish brown, light brown, dark brown, rusty, fleshy white and gradually turns violet. It is called Baekbokryeong which is white and Baekbokryeong which is red. The pharmacological actions of Byeongryeong are diuretic, antipyretic, intestinal relaxation, ulcer prevention, hypoglycemic action, increase in cardiac contractility, immunity enhancement, antitumor action and the like.

It is about 20% of the total weight of the temple. It has been reported that, in general, there is a tendency to abandon the goryeongbyeo, and that the extracts of the gyeongryeok shell can induce regulatory T cells in vivo to suppress allergies.

From the summer to autumn, Mushroom Mushroom grows on the trunk of broad-leaved trees such as oak, oak, and lignin, and is a woody white mushroom. Most mushrooms contain up to two active polysaccharides, while the mushrooms contain five kinds of active polysaccharides and exhibit excellent anticancer effects. In addition, it has been reported that it is effective for the prevention of dementia and for the improvement of diabetes by strengthening blood vessels surrounding the stomach wall. In addition, chronic enteritis, improvement of immune function, dementia suppression, such as the effect of low calorie and rich dietary fiber has been attracting attention as a healthy food.

The content of beta-glucan is higher than that of dry weight by more than 40%. Beta Glucan is a kind of polysaccharide that is present in cell wall, mushroom and cereal. It does not directly attack cancer cells but activates immune function of normal human cells through nonspecific immune reaction, inhibits proliferation and recurrence of cancer cells, activates macrophages It enters the body with cancer cells and promotes the secretion of various cytokines, thereby activating the immune functions of T cells and B cells, which are immune cells. Betaglucan, which is a major component of P. japonica, has limitations in effective ingestion and hydration by a general method. Therefore, it is preferable to carry out the particle size of 200 to 440 mesh when crushing the mushroom of the present invention.

The foundation stone sleep is a perennial plant belonging to the perennial plant belonging to the monocotyledonous plants of the dicotyledonous plant. It contains the component of phenylethanoid which activates the brain function, and it is rich in choline which can prevent dementia. Corneal sleep also helps prevent edema and stroke, improves blood circulation, improves cirrhosis and arteriosclerosis, and inhibits the formation of fatty liver. In addition, it has the effect of giving hemostasis and boil, and it is effective for the cold, headache, sore throat, bronchitis.

Hereinafter, the herbal composition for enhancing immunity of the present invention will be described in more detail through the following examples.

≪ Example 1 > Selection of microorganisms suitable for fermentation of herbal composition compositions

1-1. Strain isolation from natural products

A total of 17 kinds of fermented foods (6 kinds of cabbage kimchi, 3 kinds of mustard kimchi, 3 kinds of Dongchimi, bamboo kimchi, yamu kimchi, cucumber pickles, plums and yogurt) were used as samples. To separate the microorganisms from the fermented foods, each sample was decanted and stained with Lactobacilli MRS agar (Difco Co., USA), cultured at 37 ° C for 2 days, and the lactic acid bacteria were firstly isolated and isolated at random Were subcultured and stored at 4 ° C.

1-2. Isolate lactic acid bacteria suitable for fermentation starter

In order to identify acid-producing strains from MRS agar, lactic acid bacteria were isolated using MRS plate medium containing 0.006% BCP (Bromocresol Purple, Sigma, USA) as a pH indicator, and the purple medium of BCP was yellow , And then the lactic acid bacteria were isolated repeatedly until a pure strain was obtained.

1-3. Acid resistance analysis

The isolates were inoculated in a 0.05 M sodium phosphate buffer solution (pH 7.0) and a 0.05 M sodium phosphate buffer solution adjusted to pH 2.0 and 4.0, respectively, and then shake cultured at 37 ° C for 2 hours. The survival rate (%) was calculated by measuring absorbance at nm.

The pH of the stomach is pH 0.78 ~ 0.90 for fasting, but when the food is ingested, the pH of the gastric juice goes up to 2.0 ~ 3.0 and the time for food to pass is 2 ~ 4 hours. To demonstrate its effectiveness as a probiotic, it should survive at pH 3 or lower and reach the small intestine through the stomach. A strain with a survival rate of 70% or more at pH 2 was selected.

1-4. Analysis of biliary properties

In order to analyze the biliary properties, the isolates were inoculated 10% in MRS broth supplemented with 0.3% (w / v) oxgall filtered with Lactobacilli MRS broth and 0.45 μm membrane filter, incubated at 37 ° C for 8 hours, And the survival rate (%) was calculated.

1-5. Antibacterial activity against E. coli

The strains used for the detection of the antimicrobial activity were obtained by inoculating 0.2 to 2 mL of the test strain with aseptically added strains of 2-3 platinum strains cultured on a plate culture medium, inoculating the culture broth of 10 mL nutrient broth, incubated at 37 ° C. for 24 hours, And then applied to the sterilized glass plate to spread evenly over the substrate medium. The disc was assayed by Piddok paper disc agar method (disc plate method).

1-6. Identification of the selected strain (identification)

When the results of acid - fast bacillus and antimicrobial activity were combined, the 5-1 strain with the highest efficiency was finally selected and the final selected strains were analyzed by 16S rRNA partiall sequencing. FIG. 1 shows a phylogenetic analysis chart based on 16S rRNA sequence analysis of the strain finally selected in the present invention. As a result of microbial identification, the final selection strain 5-1 was found to be 99% similar to Leuconostoc mesenteroides subsp. Dextranicum strain (Korean Microorganism Conservation Center). In FIG. 1, the scale bar shows a 0.002% nucleotide difference.

≪ Example 2 > Preparation of herbal composition for enhancing immunity activity

2-1. Material preparation

Sparassis crispa was purchased from Hwasun, Korea, and Hericium erinaceus was purchased from Pyeongteck, Korea. Red ginseng radix , Poria cocos , Asparagi radix , Liriopis tuber , Lycii fructus , Stachys sieboldii Miq and Honey are herb medicine distribution centers (Hwasun, Korea) and Omni Hub (Daegu, Korea). Rehmanniae radix was purchased from Geumjang Agricultural Cooperative (Geumsan, Korea). All the ingredients were domestic.

Korean red mushroom, red mushroom, red ginseng, Byeongryeong, Chunmun - dong, Maekmundong, Gugija, ground stone, and raw persimmon were washed with an ultrasonic washing machine. The bark was peeled and divided into bokjungpip (15% of the ginseng) and Baekbokryeong (85% of the ginseng). Among the above materials, the solid materials such as Pseudomonasporium mushroom, Rosemary mushroom, Red ginseng, Baekbokryeong, Bokryeopi, Chunmundong, Maekmundong, Gugija and Tungmokwa were pulverized and finely pulverized. The raw persimmon was squeezed after crushing and separated into juice juice (juice ratio 60%) and persimmon leaf.

2-2. Preparation of herbal medicinal composition for enhancing immunity activity

The above prepared materials were mixed in the amounts shown in Table 1 to prepare a herbal medicinal composition for enhancing immunity activity.

≪ Example 3 > Preparation of herbal composition for enhancing immunity activity

The ingredients prepared in Example 2 were mixed in the amounts shown in Table 1 to prepare a herbal medicinal composition for enhancing immunity activity.

<Example 4> Preparation of herbal medicinal composition for enhancing immunity

4-1. Strain preparation

The yeast strains were prepared by inoculating the MRS broth with Leuconostoc mesenteroides subsp. Sp. Textanicum strain finally selected in Example 1 and then preincubating at 37 DEG C for 48 hours.

4-2. Preparation of herbal medicinal composition for enhancing immunity activity

The materials prepared above were mixed in the amounts shown in Table 1. The herbal composition for enhancing the immune activity was prepared by inoculating 2% (v / v) of the pre-cultured Reukono Stokes mesenteroides subspecifica textanicum strain at 37 ° C for 110 hours.

<Example 5> Preparation of herbal medicinal composition for enhancing immunity

The herbal composition for enhancing immunity activity was prepared in the same manner as in Example 4, except that the ingredients were mixed in the amounts shown in Table 1.

Example 6: Preparation of immunostimulating gypsum

The herbal composition for enhancing immunity prepared in Example 2 was kneaded well and placed in pot. The pot was placed in a water-filled pot. The temperature was maintained at 98 ~ 102 ℃ for 3 days using a temperature gauge (KP3001, Ziraffe, China), and the water was filled in the hot water tank to keep the pot to be locked at 80% or more. After completion of the first bath, the mixture was allowed to stand at room temperature for 1 day, and the first aging process was carried out. Then, a second bath was performed under the same conditions as the first bath for 1 day, followed by a second aging process under the same conditions as the first aging for 1 day to prepare an immunostimulating gland (IYG-gami) .

<Example 7> Preparation of immunostimulating gypsum

(M-IYG-gami) was prepared in the same manner as in Example 6 except that the herbal medicinal composition for enhancing immunity prepared in Example 3 was used.

<Example 8> Preparation of immunostimulating gypsum

(IYG-gami-F) was prepared in the same manner as in Example 6 except that the herbal medicinal composition for enhancing immunity prepared in Example 4 was used.

<Example 9> Preparation of immunostimulating gypsum

(M-IYG-gami-F) was prepared in the same manner as in Example 6, except that the herbal medicinal composition for enhancing immunity prepared in Example 5 was used.

&Lt; Experimental Example >

1-1. Experimental Method

1) Animals

Sprague Dawley rats weighing about 170-180 g were divided into two groups: a solid diet (Pellet, GMO, Korea) and water in a kennel (room temperature 24 ~ 26 ℃, humidity 40 ~ 60% After sufficient feeding, they were adapted to the laboratory environment for more than one week and used in the experiment. During the experiment, water and solid feed were also freely consumed.

2) induce immunodeficiency

The dose of methotrexate (MTX) (Sigma, USA) was determined at a concentration of 2 mg / Kg. The dose of methotrexate (MTX) Respectively.

3) Group separation (n = 5)

The experimental group was divided into a normal group that did not induce immune degradation (normal), a control group that was not treated after MTX immunodegradation induction (control), a test group A (IYG-gami) (IYG-gami-F) after the administration of the sample of Example 8, the experimental group C (M-IYG-gami) of the sample 7 administered with the MTX immunodegradation test, D (M-IYG-gami-F).

4) Sample administration

Each sample was prepared at a concentration of 200 mg / Kg and then administered with drinking water for the entire experimental period (20 days).

5) Measure weight and spleen weight

Body weights were measured on Day 1, Day 5, Day 10, Day 15, and Day 20 using an electronic balance (Cass, China). The spleen weight was measured by separating the spleen from the end of the experiment.

6) Blood and serological tests

Approximately 100 μl of the blood obtained by blood collection was placed in an EDTA-bottle and immediately injected into a Multi species Hematology Analyzer (950, Hemavet, USA) to measure Leukocytes, Erythrocytes, WBC, RBC, HGB and HCT. The remaining blood was separated from the serum by centrifugation at 3,500 rpm for 20 minutes on a high-speed centrifuge (Centricon T-42K, Italy). AST and ALT were measured by Dri-chem 4000i (Fujifilm corp. Japan) for serum analysis.

7) ELISA measurement

① TNF-a

TNF-α was measured using Rat TNF-α (Invitrogen, USA). After adding 100 μl of Rat TNF-α standard, serum, and control to a microplate coated with Rat TNF-α and tapping with a plate cover, it was mixed for 1 minute and left at room temperature for 2 hours. After washing 4 times with 400 μl of washing solution, 100 μl of Rat TNF-α Biontin Conjugate was added and the plate cover was covered and allowed to stand at room temperature for 1 hour. After washing 4 times with 400 ㎕ of washing solution, 100 ㎕ of Streptavidin-HRP working solution was added and the plate cover was covered and allowed to stand at room temperature for 30 minutes. After washing 4 times with 400 ㎕ of washing solution, 100 ㎕ of Stabilized Chromogen was added, and the plate cover was covered and left at room temperature (dark state) for 30 minutes. After stopping the color reaction, 100 μl of stop solution was added to the plate and OD (optical density) was measured at 450 nm using a microplate reader (EZ Read 400, Biochrom, US). A standard curve was made to assay the amount of TNF-α in the sample.

② Interleukin-2 (IL-2)

IL-2 measurements were performed using Rat IL-2 (R & D Systems, USA). 50 μl of Assy Diluent RD1-21 was added to each well of the microplate, and 50 μl of Rat IL-2 standard, control, and serum were added. After tapping with a plate cover, the mixture was mixed for 1 minute and left at room temperature for 2 hours. After washing 5 times with 400 μl of washing solution, 100 μl of Rat IL-2 conjugate was added and the plate cover was covered and allowed to stand at room temperature for 2 hours. After washing 5 times with 400 μl of washing solution, 100 μl of substrate solution was added, and the plate cover was covered and allowed to stand at room temperature (dark state) for 30 minutes. After stopping the color reaction, 100 μl of stop solution was added to the plate and OD (optical density) was measured at 450 nm with a microplate reader (EZ Read 400, Biochrom, US).

③ Interleukin-6 (IL-6)

IL-6 measurement was performed using Rat IL-6 (R & D Systems, USA). 50 μl of Assy Diluent RD1-21 was added to each well of the microplate, and 50 μl of Rat IL-10 standard, control, and serum were added. After tapping with a plate cover, the mixture was mixed for 1 minute and left at room temperature for 2 hours. After washing 5 times with 400 μl of washing solution, 100 μl of Rat IL-10 conjugate was added, and the plate cover was covered and allowed to stand at room temperature for 2 hours. After washing 5 times with 400 μl of washing solution, 100 μl of the substrate solution was added, and the plate cover was covered and allowed to stand at room temperature (dark state) for 30 minutes. After stopping the color reaction, 100 μl of stop solution was added to the plate and OD (optical density) was measured at 450 nm with a microplate reader (EZ Read 400, Biochrom, US).

④ Immunoglobulin E (Ig E)

Ig E measurements were measured using the Rat Ig E Elisa Kit (Abcam, UK). Each well of the microplate was filled with 100 μl of Ig E standard, control and serum, followed by tapping with a plate cover, followed by mixing for 1 minute and left at room temperature for 1 hour. After washing three times with 1 × Wash Buffer, 100 μl of 1X Enzyme-Antibody conjugate was added, and the plate cover was covered and allowed to stand at room temperature (dark state) for 1 hour. After washing three times with 1 × Wash Buffer, 100 μl of TMB substrate solution was added, and the plate cover was covered and allowed to stand at room temperature (dark state) for 10 minutes. After stopping the color reaction, 100 μl of stop solution was added to the plate and OD (optical density) was measured at 450 nm using a microplate reader (EZ Read 400, Biochrom, US).

1-2. Statistical processing

All measurements were expressed as mean ± standard error using the Excel statistic program (Microsoft, USA). Statistical analysis was performed using SPSS for Windows (SPSS, USA) Mann-Whitney U test was performed. The significance of each test group was tested at α = 0.05 level (P <0.05) and α = 0.01 level (P <0.01) compared to the control group.

1-3. Experiment result

1) Effect on TNF-α

TNF-α levels were significantly increased in the control group without immunodegradation than in the control group without immunodeficiency. The experimental groups A to D in which the herbal medicinal composition for immunostimulatory activity of the present invention was administered showed a significant decrease as compared with the control group (Table 2 and Fig. 2).

2. Effects on Interleukin-2 (IL-2)

IL-2 showed a significant increase in the control group as compared with the normal group, and a significant decrease was shown in the experimental group compared to the control group (Table 3 and Fig. 3).

3. Effect on Interleukin-6 (IL-6)

IL-6 showed a significant increase in the control group as compared with the normal group, and a significant decrease was observed in the experimental group compared to the control group (Table 4 and Fig. 4).

4. Effect on Immunoglobulin E (IgE)

IgE showed a significant decrease in the control group compared with the normal group, and the experimental groups B, C, and D showed a significant decrease compared to the control group (Table 5 and FIG. 5).

5. Effect on spleen weight

As a result of observing the effect of the herbal medicine composition according to the present invention on the spleen weight change, 0.83 ± 0.03 g in the normal group and 0.71 ± 0.03 g in the control group were significantly decreased compared to the control group, and the IYG In the case of M-IYG-gami group, 0.78 ± 0.06 g, 0.78 ± 0.05 g in IYG-gami-F group, 0.78 ± 0.03 g in M-IYG-gami group and 0.81 ± 0.02 g in M-IYG-gami- The IYG-gami-F group showed a significant increase (Table 6 and Fig. 6).

6. Effect on blood leukocytes content change

As a result of observing the effects of the herbal composition composition of the present invention and the fermentation thereof on the change of blood leukocytes, the IYG-gami-F group was significantly increased in WBC and lymphocytes compared to the control group (Table 7 and FIG. 7).

7. Effect on blood erythrocytes content change

As a result of observing the effect of the herbal composition composition of the present invention and the fermentation thereof on the blood erythrocytes, the MCV was significantly decreased in the experimental group and the IYG-gami group was decreased in the MCH group (Table 8 and FIG. 8).

8. Effect on weight change

As a result of observing the effects of the herbal composition composition and the fermentation of the present invention on the change in body weight, the IYG-gami-F group showed a significant decrease in the control group on the 15th day and 20th day compared to the control group, Day, and the M-IYG-ami-F and M-IYG-gami-F groups showed a significant increase at 15 days (Table 9 and Fig. 9).

9. Effect on serum aminotransferase content

This study was carried out to investigate the effects of fermented soybeans on the quality of the fermented soybeans and fermented soybeans, (AST) was 75.6 ± 3.4 U / l in the normal group, 86.6 ± 6.6 U / l in the control group, and IYG-gami group in the control group, as a result of the changes in the serum aminotransferase content 83.8 ± 3.2 U / l, 83.2 ± 4.4 U / l for the IYG-gami-F group, 88.6 ± 9.7 U / l for the M-IYG-gami group and 83.8 ± 6.3 U / l for the M-IYG- In the case of alanine aminotransferase (ALT), 43.6 ± 3.5 U / l in the normal group, 47.7 ± 4.4 U / l in the control group and 42.6 ± 3.2 U / l in the IYG-gami group I / GAMI-F group was 40.8 ± 2.7 U / l, M-IYG-gami group was 46.2 ± 7.0 U / l and M-IYG-gami-F group was 45.2 ± 1.8 U / There was no significant change in the experimental group ( Table 10 and Fig. 10).

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.

Claims (3)

Herbaceous mushroom, red mushroom, red ginseng, Byeongryong, Chunmun-dong, mammun-dong, gugija, cornstarch sleep, raw persimmon and rosemary.
The method according to claim 1,
The herbal medicine composition for immunostimulatory enhancement according to the present invention is characterized in that it comprises 1 to 7% by weight of mushroom, 0.5 to 5% by weight of mushroom, 2 to 10% by weight of red ginseng, 5 to 15% 0.5 to 5% by weight of corn syrup, 0.5 to 5% by weight of corn syrup, 0.5 to 5% by weight of cornstarch, 30 to 53% by weight of raw persimmon and 25 to 40% by weight of corn syrup.
The method according to claim 1,
Wherein the immunoactivity enhancing herbal composition is fermented with a strain of Leuconostoc mesenteroides subsp. Dextranicum .

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