KR100860209B1 - A functional feed excipient using sulfur and manufacturing process of the same and a pork acquired therefore - Google Patents

Abstract

A functional feed supplement containing herbs comprising citrus peel powder, dandelion powder and the like and sulfur is provided to improve meat quality by remarkably reducing saturated fatty acid content and cholesterol content in pork when fed to a pig. A functional feed supplement contains: herbs comprising 27% by weight of citrus peel powder, 2.4% by weight of catechin powder, 4.2% by weight of dandelion powder, 6.9% by weight of Magnolia Bark powder, 4.1% by weight of Atractylodes Root powder, 4.1% by weight of Poria cocos powder, 4.1% by weight of Epimedium leaf powder, 3.5% by weight of Leonurus Leaf powder, 3.5% by weight of Perilla Leaf powder, 3.5% by weight of Areca Seed powder, 3.5% by weight of Spirodela polyrhiza powder, 3.5% by weight of Raphanus Seed, 3.5% by weight of Bambusae caulis in Taeniam, 3.5% by weight of eggplant powder, 3.5% by weight of Lotus Seed powder, 5% by weight of Licorice Root powder, 4.1% by weight of Cinnamomum Bark powder, 2.5% by weight of oyster shell powder and 3.5% by weight of Crataegus Fruit powder; and 4.1% by weight of sulfur. The functional feed supplement is fed to pigs to obtain pork in which 11% by weight of total cholesterol, 3 to 4% by weight of neutral lipid, 5 to 5.5% by weight of HDL-cholesterol and 6% by weight of LDL-cholesterol are reduced.

Description

FUNCTIONAL FEED EXCIPIENT USING SULFUR AND MANUFACTURING PROCESS OF THE SAME AND A PORK ACQUIRED THEREFORE}

The present invention relates to a functional feed additive using sulfur-containing medicinal herbs and to pigs produced and produced with feed containing the same, and more specifically, a tangerine peel (dermis) and pogongyoung (19) specifically selected 19 When mixed with various kinds of powdered herbal medicines at a predetermined ratio, sulfur powder is added and mixed at a predetermined ratio, and then added at a predetermined ratio to the feed to be fed at the time of pig breeding to feed the pigs, Functional feed additives using sulfur-containing medicinal herbs to improve the growth improvement effect and significantly reduce the unsaturated fatty acid content and cholesterol content in pork, and produce and produce pork with the added feed. It is about.

In general, pork among many kinds of livestock is cheap and contains high quality protein.

On the other hand, as the income level is advanced, consumers' meat preference is getting more attention than the quantity, and the global market is also unifying as the tariff policy is rapidly progressed.

Therefore, the qualitative differentiation of meat is urgent as the research on quality improvement is as good as securing price competitiveness in livestock production.

Meanwhile, exports of processed foods have emerged as an important industry, and in order to improve their quantity, growth-promoting antibiotics have been frequently injected into livestock feed.

In the case of raising livestock by adding antibiotics to the feed, antibiotics inevitably remain in the meat.Resistant bacteria that have increased drug resistance in the human body by continually ingesting meat containing the residual antibiotics above the allowable threshold This emergence can lead to serious side effects, including the difficulty of treating the disease. Currently, most of the bacteria scattered in domestic livestock are resistant to conventional antibiotics, and the treatment thereof becomes increasingly difficult. The antibiotic resistance expression of bacteria is expressed in almost all drugs that are used without appearing in some localized antibiotics. Genetic and genetic characterization has shown the ability to resist any drug.

The European Parliament has amended the Council Directive 70/524 / EEC (`11/23/70) of the Feed Additives (December 17, 1998) and used them for feed additions. '' Among the antibiotics allowed, four kinds of antibiotics, zinc-bacitracin, spiramycin, phosphotyrosine, and virginiamycin, were prohibited. Four antibiotics for feed additives, including Pulabophosphoripol (Fulabomycin), Monensinsodium, Salinomycin Sodium, and Avilamycin, will continue to be permitted for use, but this will also be regulated from 2006.

Therefore, in order to solve various serious problems derived from these residual antibiotics, it is urgently needed to develop a new antibiotic having a structure that does not cause resistance and understands the current mechanism of resistance. As a mixture, it shows the pharmacological action between the components and has been used for the treatment and prevention of diseases of the human body for a long time in Korea and other Asian countries. Efforts are being made.

On the other hand, as cardiovascular diseases such as obesity, hypertension, cerebrovascular disease, arteriosclerosis, etc. increase rapidly due to the increase in fat intake of meat, more people avoid foods with high saturated fatty acid and cholesterol content such as pork. have.

As a result, meat quality is improved to reduce the saturated fatty acid content and cholesterol content in meat, thereby improving consumer's preference, showing the effect of immune activity and growth and improving the productivity of livestock and raising livestock industry internationally. There is an urgent need for the development of functional feed additives to ensure competitiveness.

The present invention was developed by the necessity as described above, and its purpose is to mix 19 kinds of specially selected herbal medicines such as dermis and pogongyoung at a specific ratio, and sulfur powder in a certain ratio. When it is added and mixed, it is added to the general feed that is fed during the breeding of pigs, and when it is fed to pigs, it can greatly enhance the immune activity and growth improvement effect of pigs, and significantly increase the saturated fatty acid content and cholesterol content of pork. It is an object of the present invention to provide a functional feed additive using a herbal medicine containing sulfur, which reduces and significantly improves the meat quality of pork.

It is also an object of the present invention to provide a pork which significantly improves meat quality by significantly reducing the saturated fatty acid content and cholesterol content in pork produced by breeding pigs with the feed to which the functional feed additive is added.

In order to achieve the above object, the functional feed additive according to the present invention is 27% by weight dermis powder, catechin powder 2.4% by weight, dandelion powder 4.2% by weight, thick gourd Iii)) 6.9% by weight powder, 4.1% by weight of shovel (creation) powder, 4.1% by weight of Bokryeong powder, 4.1% by weight of trichophytic vinegar (Yin Yang-gwa) powder, motherwort powder 3.5% by weight, device powder 3.5% by weight, betel nut 3.5% by weight, frog rice (Bupyeongcho) powder 3.5% by weight, radish powder (3.5) 3.5% by weight of Genus bark powder, 3.5% by weight of eggplant powder, 3.5% by weight of lotus seed powder, 5% by weight of licorice powder, gage (桂枝) 4.1% by weight of sulfur powder in Chinese herbal medicine consisting of 4.1% by weight powder, 2.5% by weight of oyster shell powder, and 3.5% by weight of sansa powder. There is a characteristic.

In order to achieve the above another object, pork of pigs bred with the feed containing the functional feed additive using sulfur-containing herbal medicine according to the present invention is 27% by weight of dermis powder, catechin powder 2.4 weight %, Dandelion powder 4.2% by weight, thick thin powder 6.9% by weight, shoveled powder 4.1% by weight, Fuling powder 4.1% by weight, trigeminal leaf vinegar ( Yin-Yang Kwak powder 4.1% by weight, motherwort powder 3.5% by weight, soybean powder 3.5% by weight, betel nut 3.5% by weight, frog rice (Bupyeong vinegar) powder 3.5% by weight, radish seed, 3.5% by weight, reddish husk powder, 3.5% by weight, eggplant powder, 3.5% by weight, lotus seed 3.5% by weight of powder, 5% by weight of licorice powder, 4.1% by weight of gage powder, 2.5% by weight of oyster shell powder, 3.5% by sand powder Oil in Chinese Herbal Medicine Functional feed additives containing 4.1% by weight of sulfur powder were fed for 6 weeks with 0.7% by weight of general feed, compared to only customized feed, 11% of total cholesterol and neutral lipid in meat. 3% to 4%, 5% to 5.5% for HDL-cholesterol, and 6% for LDL-cholesterol.

Here, the compounding rate of each element of the general feed is as follows.

Hereinafter, preferred embodiments of the present invention will be described in more detail.

Functional feed additives using sulfur-containing medicinal herbs according to the present invention greatly improve the immune activity and growth improvement effect of livestock when fed to pigs by adding them at a predetermined ratio in a customized feed, saturated fatty acid content and cholesterol in slaughtered pork Significantly reduced content to improve meat quality.

To this end, the functional feed additive using a sulfur-containing herbal medicine according to the present invention dermis powder, catechin powder, dandelion powder, thick powder, shovel (creation) (,)) Powder, Furyeong powder, Samji-gu leaf herb powder, motherwort powder, soja powder, betel nut, frog rice (Bupyeongcho) Powder, radish seed, nachoja powder, ranch shell powder, eggplant powder, lotus seed powder, licorice Chinese herbal medicine consisting of powder, sesame powder, oyster shell powder, and sansa powder is added and mixed at a predetermined ratio, and sulfur powder is added thereto. It is made by mixing at a certain ratio.

The catechin (Catechin) of the herbal ingredients applied to the present invention occupies the highest content among the components of tea, and is added at a ratio of 2.4% by weight as a flavoring ingredient, and dermis powder is a tangerine The skin is dried and made into powder. The dermis is the better name for long-term stay. It is used to protect the stomach and to help digestion. Is added in proportion.

In addition, pogongyoung (蒲公英) of the herbal medicine applied to the present invention refers to the dandelion to lower heat poisoning and boil swelling, swelling, mastitis, sore throat, carbuncle (sentitis, pulmonary abscess, peritonitis) and write eye ocular blood, acute hepatitis, jaundice This can be used for symptoms that prevent the person from urinating due to fever. As a pharmacological action, fungi, immune function, biliary secretion, hepatoprotective action, and diuretic action have been reported and added at a rate of 4.2% by weight.

In addition, the medicinal herbs applied to the present invention is used for the treatment of medicinal herbs, kwakran, vomiting, diarrhea, etc. in herbal medicine, and dried to make powdered bark of bark It is added at the ratio of 6.9 weight%.

In addition, the creation of the herbal medicine applied to the present invention refers to the roots or stems of shovels, which are perennial plants of the Asteraceae, and are effective in sweating, diuresis, pain, health, etc. and are used for anorexia, dyspepsia, gastroenteritis, and cold. It is added as a medicine at a ratio of 4.1% by weight.

In addition, Bokryong (적용) of the herbal medicine applied to the present invention is a mushroom that is parasitic on the roots of pine trees in the ground in the form of balls or ovals, and has the effect of diuresis. It is added in the ratio of 4.1% by weight.

In addition, the yinyanggwa (한) of the herbal medicine applied to the present invention refers to trichophytic herb (三 枝 九 葉 草), blood pressure drop, coronary blood flow increase, blood sugar drop, cholesterol drop, immune function enhancement, Jinhae, expectoration, Pyeongcheon It is reported that it has the effect of sedation, fungus, anti-inflammatory action, chicken femur growth and protein polysaccharide synthesis, and is added at a ratio of 4.1% by weight.

In addition, motherwort herb (益母草) of the herbal ingredients applied to the present invention is also called hair growth herb and is used for postpartum hemostasis and abdominal pain and is a medicine that has a blood pressure-lowering, diuretic, soothing, analgesic effect. Motherwort is added at a rate of 3.5% by weight.

In addition, the element of the herbal medicine applied to the present invention refers to a perilla, it is effective in sweating, antipyretic, analgesic, enteritis, digestion, poisoning of fish meat, etc., and is added at a rate of 3.5% by weight.

In addition, betel palm (Betel palm) of the herbal ingredients applied to the present invention is used for diarrhea symptoms or insect repellent in tangerine and alkaloids, which is dried fruit powder to make betel nut at a rate of 3.5% by weight. Add.

In addition, Bupyeongcho (개구리 草) called frog rice of the herbal medicine applied to the present invention is used for diuresis, sweating, rubella toxicosis, angioedema, sweating, diuresis, persimmon, swelling, sinusitis, rhinitis, bronchial and dried powder 3.5 It is added in the ratio of weight%.

In addition, nabokja (무 子), which refers to radish among the herbal ingredients applied to the present invention, is used for gastritis and bronchitis as a dry stomach and expectorant, and the powder made by drying it is added at a rate of 3.5% by weight.

In addition, among the medicinal herbs applied to the present invention is a powder of pulverized by drying the inner skin of the cotton pad is killing (竹茹) powder is known to have the effect of stopping the heat and squirming and to break the wall, but in the present invention the ratio of 3.5% by weight Is added.

In addition, Gaza (茄子) of the herbal ingredients applied to the present invention refers to eggplant, improves anemia, bleeding symptoms, lowers the amount of cholesterol in the blood, promotes the function of the liver and pancreas, has a diuretic effect, eggplant Of scopoline, scoparon has the efficacy of jingyeong and is added in the present invention at a rate of 3.5% by weight.

In addition, among the herbal medicine ingredients applied to the present invention, lotus leaf (蓮 子 肉) refers to the seeds of lotus, and in Chinese medicine, the spleen is mostly used for fragile symptoms to stop diarrhea, and the chest beats and treats the symptoms of insomnia. It is also effective in supplementing renal function and adds 3.5% by weight to the present invention.

In addition, licorice (甘草) of the components of the herbal medicine applied to the present invention neutralizes and detoxifies, which acts to neutralize and alleviate the poison of all drugs, so it is used as an indispensable herbal medicine in herbal medicine, and in the present invention, 5 weight Add in percentage.

In addition, Gyeji (지) of the herbal medicine applied to the present invention refers to the branches of cassia and inhibits the growth of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pneumococcal pneumoniae, etc., and has a pharmacological effect of inhibiting influenza virus. It is dried, made into powder, and added at a ratio of 4.1% by weight. Mosul powder is a powder made by burning oyster shells, which is slightly cold, and used as a medicine or as a medicine for fever or thirst. It is added in% by weight. Sansa has the highest component of protein fat ash, contains vitamin C, citric acid, pentosan, oxalic acid, calcium, etc. It promotes to help digestion, and is effective in stomach pain due to food poisoning, meat poisoning, etc. In the present invention, it is added at a rate of 3.5% by weight.

On the other hand, sulfur contained in the herbal medicine of the present invention constitutes a variety of important parts of organic substances, amino acids containing sulfur such as cysteine, methionine, taurine are the main components of hormones, enzymes, coenzymes as sulfases, dermal preparations, The same applies to the preparation of a number of preparations, pesticides, solvents, in the present invention is added in a ratio of 4.1% by weight.

The functional feed additive of the Chinese herbal medicine containing sulfur mixed and added at a predetermined ratio as described above is added to the customized feed at a rate of 0.5% by weight to feed pigs for 45 days, and the functional feed additive of the present invention is not added. Compared to pigs fed under the same diet, the total cholesterol in pork decreased by 11%, triglycerides by 3-4%, HDL-cholesterol by 5-5.5%, and LDL-cholesterol by 6%.

 <Example>

1. Preparation of functional feed additive using herbal medicine containing sulfur

27 g of dermis powder, 2.4 g of catechin powder, 4.2 g of dandelion powder, 4.2 g of thick powder, 6.9 g of shochu powder, 4.1 g of shovel powder, 4.1) powder 4.1g, Samjigu leaf vinegar 4.1g, motherwort powder 3.5g, soybean powder 3.5g, betel nut 3.5g, frog rice (Bupyeong vinegar) 3.5g powder, 3.5g radish seed powder, 3.5g musk powder, 3.5g red-shell (powder) powder, 3.5g eggplant powder, lotus seed Chinese herbal medicine consisting of 3.5g powder, licorice powder 5g, cinnamon powder 4.1g, oyster shell powder 2.5g, Sansa powder 3.5g 4.1 g of sulfur powder was mixed in to prepare a functional feed additive of the present invention.

2. Preparation of extract of functional feed additive using herbal medicine containing sulfur

20 g of dried powder of the functional feed additive using a sulfur-containing herbal medicine was added to a 100% methanol solution in an amount equal to three times its weight, and allowed to stand at room temperature for 48 hours to extract the extract. 0.3 g of extract was obtained by filtration and lyophilization.

Test Example

-Antibacterial activity test of functional feed additive extract of the present invention-

(1) Disclosed strains and culture with strains

Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus KCTC 1916 Escherichia coli (Methicillin-resistant Staphylococcus aureus) as a test strain used to measure the antimicrobial activity of the functional feed additive extract of the present invention Escherichia coli) 0157 ATCC 43894, Salmonella typhimurkum Four pathogenic microorganisms of KCTC 1925 were passaged and used.

The test strains were inoculated in Tryptic Soy broth® (TSB; Difoco USA) and incubated for 24 hours at 37 ° C. incubator, and used for the experiment, using Tryptic Soy Agar® (TS; Difoco USA) medium for visual observation. It was.

(2) antimicrobial activity

Antibacterial activity was performed by investigating the change of viable cell number of each bacteria in the medium to which the functional feed additive extract using the herbal medicine containing sulfur was added.

Test strains were cultured in about 10 hours of TSB medium in about 12 hours, and then inoculated with 0.1 ml of the bacterial solution in 10 mL of TSB medium and cultured for about 12 hours. After diluting each of the disclosed strains to 108cells / ml in TSB medium added for each 10mg / ml concentration, the cells were inoculated and cultured for 24 hours, and the viable cell count of the culture was measured.

Validation of HMG-CoA Reducing Element Inhibitory Activity in the Laboratory

(1) Yeast culture and production of microsomal protein

Saccharomyces cerevisiac ATCC 96519 was purchased, and 1% glucose, 0.5% polyperpeptone, and 1% yeast extract were used to culture the strain. The medium was prepared by addition, and inoculated with the strain in the prepared medium, and pre-cultured for 24 hours under semi-anaerobic (semianaerobic) conditions of 30 ℃.

1% of the pre-cultured strain was inoculated in a fresh medium containing 3% glucose, 0.5% polypeptone, and 0.5% KH2PO4 and incubated for 15 hours at 30 ° C. After centrifugation at rpm, the collected cells were washed twice with sterile distilled water. The obtained cells were diluted to 10% (w / v) in 0.1M Triethanolamine buffer (ph 7.4) containing 20mM EDTA.

The diluted cells were homogenized using glass beads, and then centrifuged at 8,000 rpm for 15 minutes at 4 ° C. to remove mitochondria. The supernatant from which the mitochondria was removed was ultracentrifuged at 34,000 rpm for 90 minutes at 4 ° C. to obtain particulate protein.

The thus obtained particulate protein was washed with 0.1 mM triethanolamine buffer (pH 7.4) containing 20 mM EDTA and 2 mM DTT, and then stored at -70 ° C. to quantify the protein by Bradford method before use. The concentration was diluted and prepared at 10 mg / ml and used for measuring enzyme activity.

(2) Determination of inhibitory activity against HMG-CoA reducing element

Inhibitory activity against HMG-CoA reducing element was determined by modifying the spectroscopic method as follows. HMG-CoA 150mM, NADP 2M, G-6-phosphate 3M, G-6-phosphate dehydronase 2 units were prepared, 1 mg of particulate protein was added, and 100 µl (10 mg / ml) of the functional feed additive extract was added thereto. Added to maintain a final volume of 1 ml.

After reaction at 37 ° C. for 30 minutes, 20 μl of 10 mM sodium arsenate was added, and after 1 minute, 0.1 ml of 2M citrate buffer (containing 3% tungsten salt) was added and reacted at 37 ° C. for 10 minutes. .

After the reaction, the reaction solution was centrifuged at 15,000 rpm for 5 minutes to take 1 ml of supernatant, 0.2 ml of 2M Tris buffer (pH 10.6) and 0.1 ml of 2M Tris buffer (pH 8.0) were added to adjust the pH of the reaction solution to 8.0. 50 μl of 0.4M arsenate was added thereto, followed by reaction for 5 minutes to form CoA-SH, a diacid- dirhiol-arsenate complex, and 1 ml of this reaction solution was cuvetted with 1 ml. After transferring to cuvette, 20 μl of 3 mM DTNB was added, and the absorbance was measured at 412 nm.

The interpolation value was obtained by extrapolating the straight line portion from the Absorbance / Time curve, and the amount of CoA-SH was determined by opposing the following equation.

nmole / min = A (reaction) -A (control) /0.136×1.43

1.43 = dilution factor

0.136 = extinction coefficient of CoA-SH

-Verification of Meat Improvement Effect of Pork by Application of Feed Additives-

Functional feed additive using the sulfur-containing herbal medicine of the present invention prepared in the above Example was applied to the unit animal in the following manner.

(A) Test animals: 60 pigs of average weight of 45 ~ 60㎏ shall be selected for 60 days each of control and feeders, and raised for 45 days.

(B) Test feed: A dietary feed additive using the above-mentioned sulfur-containing medicinal herbs was added to the general feed at 0.7 wt%.

(C) Blood sampling and analysis: After the experiment is completed, 7 ml of the suspension is collected from the jugular veins of the control pigs and the control pigs with a heparinized syringe, and the supernatant is separated using a centrifuge. Total cholesterol, HDL-cholesterol, and triglyceride (TG) were measured by Kit (Asan Pharmaceutical, Korea), and LDL-cholesterol was determined by {Friege-wald} {total cholesterol- (HDL-cholesterol). + TG / 5)}.

Test result

(1) Test result of antimicrobial activity of pathogenic microorganisms

Extracts of functional feed additives using sulfur-containing herbal medicines according to the present invention were 4% methicillin-resistant Staphylococcus aureus (Methicillin-) of 4 pathogenic microorganisms at concentrations of 0 mg / ml, 5 mg / ml and 10 mg / ml. resistant Staphylococcus aureus, Staphylococcus aureus KCTC 1916, Escherichia coli 0157 ATCC 43894, Salmonella typhimurkum KCTC 1925, and then treated at 24 ° C at 37 ° C. After incubation for a time, the viable cell count was measured by diluting the viable cell count as a TSA plate medium.

Table 1 below shows the change in viable cell counts of test strains by the functional feed additive extract using sulfur-containing medicinal herbs. Based on this, the inhibition rate according to the concentration of the sample treated in each test strain is shown in Table 2 below. Indicated.

As shown in Table 2, the inhibition rate according to the concentration of the extract in the methicillin-resistant Staphylococcus aureus strain showed 37% inhibition when added 5mg and 87% when 10mg added. However, the Staphylococcus aureus strain showed a stronger antimicrobial activity effect than the methicillin resistant strain showing an inhibition rate of 67% at the time of addition, 100% at the addition of 10mg than when not added.

On the other hand, when the number of viable cells of Escherichia coli 0157 was observed, the inhibition rate of 51% and 75% of 5 and 10 mg, respectively, and the inhibition rate of Salmonella typhimurium were 62, respectively. % And 75% showed growth inhibition effect.

Table 1. Determination of Pathogenic Bacteria Growth Viable Counts with Functional Feed Additive Extracts

 Strain Viable cell count (CFU / mL) Sample concentration 0mg / ml 5mg / ml 10mg / ml Methicillin-resistant Staphylococcus aureus 2.1 × 10 ⁸ 1.5 × 10 ⁵ 3.1 × 10 ¹ Staphylococcus aureus KCTC 1916 1.8 × 10 ⁸ 2.1 × 10 ³ - Escherichia coli 0157 ATCC 43894 2.3 × 10 ⁸ 3.5 × 10 ³ 2.9 × 10 ² Salmonella typhimurkum KCTC 1925 1.1 × 10 ⁸ 5.9 × 10 ³ 1.2 × 10 ²

Table 2. Inhibition Rate of Pathogenic Bacteria Growth Inhibition of Functional Feed Additive Extracts

 Strain Pathogenic microbial growth inhibition rate (%) Sample concentration 5mg / ml 10mg / ml Methicillin-resistant Staphylococcus aureus 37 87 Staphylococcus aureus KCTC 1916 63 100 Escherichia coli 0157 ATCC 43894 51 75 Salmonella typhimurkum KCTC 1925 62 75

(2) Measurement result of inhibitory activity of HMG-CoA reductase

HMG-CoA reductase inhibitory activity using lovastatin (Lovastatin), which is used as a drug for cholesterol lowering by inhibiting the activity of HMG-CoA reducing element as an extract and a control of the functional feed additive using the herbal medicine containing sulfur according to the present invention Was observed.

As a result of measuring the HMG-CoA reductase inhibitory activity according to the same concentration of the extract of the functional feed additive and lovastatin, the extract of the functional feed additive using a herbal medicine containing sulfur as shown in Table 3 below, 0.5 mg, 1 mg, When 2 mg was added, the inhibitory activity was 28%, 54%, and 81%, respectively. When 0.5 mg, 1 mg and 2 mg of lovastatin were added, the inhibitory activity was 39%, 58%, and 77%, respectively. The higher the inhibitory activity was observed.

Inhibition rate of HMG-CoA reductase of extracts and lovastatin of functional feed additives using sulfur-containing herbal medicines according to the present invention was observed to have the same effect at the same concentration.

Table 3 HMG-CoA Reductase Inhibitory Activity of Extracts from Functional Feed Additives

sample Specific activity (pmoles / min / mg protein) Degree of inhibition (%) Extract 0.5mg 139.2 28 Extract 1.0mg 90.2 54 2.0mg extract 37.8 81 Lovastatin 0.5mg 119.2 39 Lovastatin 1.0mg 81.2 58 Lovastatin 2.0mg 46.1 77 Control 193.2 -

In order to observe the effect of the administration of the functional feed additive using the sulfur-containing herbal medicine according to the present invention on the serum cholesterol concentration of pigs, the ratio of the functional feed additive of the present invention prepared in Example to 0.7% by weight of general feed After supplementation, the pigs were fed to the pigs in the feed group for 45 days.

There were no significant differences in the weight gain and dietary intake of the hog pigs in the control group and the control group during the experimental period (data not shown).

Table 4 below shows the effects of serum serum cholesterol on feed by feed, and the total cholesterol in pig serum was 11% according to the feed supplemented with functional feed additive using sulfur-containing herbal medicine according to the present invention. , Neutral lipid was 3 ~ 4%, HDL-cholesterol was 5 ~ 5.5%, LDL-cholesterol was reduced by 6%.

Table 4 Effect of Pig Serum Cholesterol on Dietary Supplements with Functional Feed Additives

Control Sample salary Total Cholesterol (mg / dl) 99.4 ± 5.2 88.7 ± 5.8 Neutral Lipid (mg / ㎗) 56.1 ± 1.3 52.5 ± 2.4 HDL-cholesterol (mg / dl) 63.2 ± 4.3 58.9 ± 3.2 LDL-cholesterol (mg / dl) 25.0 ± 2.2 19.3 ± 1.8

As can be seen in the above embodiment, the functional feed additive using the sulfur-containing herbal medicine according to the present invention shows an antimicrobial activity against pathogenic microorganisms, and therefore, it is judged to have an antibiotic replacement effect when used as a feed additive for livestock and poultry. do.

In addition, in order to lower the serum cholesterol concentration, a method of inhibiting in vivo cholesterol biosynthesis has been reported as a very appropriate method, and the examination of cholesterol biosynthesis inhibitors outside the in vivo (laboratory) has been used to develop lipid metabolism control substances. HMG-CoA reductase inhibitory activity was measured as reported to be an effective and effective method, and as a result of measuring the effect of meat improvement through the serum cholesterol lowering effect by feeding pigs, sulfur-containing herbal medicine according to the present invention Inhibition rate of HMG-CoA reductase of extract and lovastatin of the functional feed additive using showed the same effect at the same concentration (Table 3).

In addition, as a result of supplementing the actual feed with a functional feed additive using a sulfur-containing herbal medicine according to the present invention to the general feed, the total cholesterol content is 22%, LDL-cholesterol as compared to the pigs that were fed only the general feed The result was 6% lower.

Taken together, these results suggest that modern cardiovascular diseases such as obesity, hypertension, cerebrovascular disease, and atherosclerosis are increasing due to increased lipid intake. Increasingly, the antimicrobial activity and meat quality improvement effect of the feeds used in this study can be applied to pig feed to increase the added value of the feed industry.

When the present invention is applied, when the functional feed additive using the herbal medicine containing sulfur is added to the general feed at a predetermined ratio, the antimicrobial activity against pathogenic microorganisms is shown, and thus it is effective to replace antibiotics when used as a feed additive for livestock and poultry. .

In addition, the HMG-CoA reductase inhibitory activity was measured, and the effect of meat improvement through the serum cholesterol lowering effect by feeding pigs, and the results of the extract of the functional feed additive and lovastatin using the herbal medicine containing sulfur according to the present invention Inhibition rate of HMG-CoA reductase has the same effect at the same concentration, and as a result of feeding the actual feed additives using a functional feed additive using a sulfur-containing herbal medicine according to the present invention to the custom feed, the control of the control broiler fed only the general feed Compared with pigs, the total cholesterol content is 11% and LDL-cholesterol is 6%, resulting in a marked improvement in the meat quality of pigs.

Therefore, pork obtained by the present invention can prevent cardiovascular diseases such as obesity, hypertension, cerebrovascular disease, arteriosclerosis, etc., and when the functional feed additive according to the present invention is applied to a pig feed, it can increase the added value to the feed industry. It works.

Claims (2)

27% by weight of dermis powder, 2.4% by weight of catechin powder, 4.2% by weight of dandelion powder, 6.9% by weight of thick powder, injected powder 4.1 wt%, Bokryeong powder 4.1 wt%, trichophytic vinegar 4.1 wt%, motherwort powder 3.5 wt%, element powder 3.5 wt%, betel nut 3.5% by weight of brown rice, 3.5% by weight of duck rice, Bupyeongcho powder, 3.5% by weight of radish (nabokja) powder, 3.5% by weight of husk shell powder, eggplant ( 3.5% by weight powder of Gaza, 3.5% by weight of lotus seed powder, 5% by weight of licorice powder, 4.1% by weight of gazette powder, shell of oyster shell Iv)) Functional feed additive using sulfur-containing medicinal herbs, characterized in that the Chinese herbal medicine consisting of 2.5% by weight powder, 3.5% by weight of Sansa powder, 4.1% by weight of sulfur powder.       27% by weight of dermis powder, 2.4% by weight of catechin powder, 4.2% by weight of dandelion powder, 6.9% by weight of thick powder, injected powder 4.1 wt%, Bokryeong powder 4.1 wt%, trichophytic vinegar 4.1 wt%, motherwort powder 3.5 wt%, element powder 3.5 wt%, betel nut 3.5% by weight of brown rice, 3.5% by weight of duck rice, Bupyeongcho powder, 3.5% by weight of radish (nabokja) powder, 3.5% by weight of husk shell powder, eggplant ( 3.5% by weight powder of Gaza, 3.5% by weight of lotus seed powder, 5% by weight of licorice powder, 4.1% by weight of cinnamon powder, shell of oyster Iv)) The functional feed additive using a sulfur-containing herbal medicine, characterized in that the sulfur powder 4.1% by weight of the Chinese herbal medicine consisting of 2.5% by weight powder, 3.5% by weight of Sansa powder 45 days with 0.7% by weight of feed While paid to pigs;       Chinese herbal medicine containing sulfur, characterized by a decrease of 11% in total cholesterol, 3 ~ 4% in neutral lipids, 5 ~ 5.5% in HDL-cholesterol and 6% in LDL-cholesterol, compared to pigs fed only feed. Pork raised and produced as a feed with functional feed additives.