KR20190074722A - Novel shiga toxin producing Escherichia coli specific bacteriophage ECOH7 and antibacterial composition comprising the same - Google Patents

Novel shiga toxin producing Escherichia coli specific bacteriophage ECOH7 and antibacterial composition comprising the same Download PDF

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KR20190074722A
KR20190074722A KR1020170176292A KR20170176292A KR20190074722A KR 20190074722 A KR20190074722 A KR 20190074722A KR 1020170176292 A KR1020170176292 A KR 1020170176292A KR 20170176292 A KR20170176292 A KR 20170176292A KR 20190074722 A KR20190074722 A KR 20190074722A
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김영주
장봉근
한범구
김현일
박철세
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Abstract

The present invention relates to a novel bacteriophage ECOH7 specific to Shiga toxin-producing Escherichia coli and, more specifically, to an antibacterial composition comprising the bacteriophage ECOH7 specific to Shiga toxin-producing Escherichia coli. According to the present invention, the bacteriophage ECOH7 shows extremely high specificity to Shiga toxin-producing Escherichia coli; may be transfected into Shiga toxin-producing Escherichia coli to propagate inside the Shiga toxin-producing Escherichia coli, thereby showing excellent bacteriolytic activities; and is highly resistant to physicochemical stimuli. In addition, the bacteriophage ECOH7 of the present invention does not transfect hosts other than bacteria, such as humans, animals, and plants, and thus serves to address the problems associated with antibiotic-resistant bacteria due to overuse and misuse of antibiotics, the problems associated with antibiotics remaining in food, and the problems associated with a broad range of hosts. Thus, the bacteriophage ECOH7 may be widely used in the fields relating to antibiotic compositions, feed additive compositions, feed, disinfectants, or detergents.

Description

신규한 시가톡신 생성 대장균 특이 박테리오파지 ECOH7 및 이를 포함하는 항균 조성물{Novel shiga toxin producing Escherichia coli specific bacteriophage ECOH7 and antibacterial composition comprising the same}A novel cytotoxin-producing E. coli-specific bacteriophage ECOH7 and an antimicrobial composition comprising the same, wherein the microorganism is Escherichia coli specific bacteriophage ECOH7 and antibacterial composition comprising the same,

본 발명은 신규한 시가톡신 생성 대장균 특이 박테리오파지 ECOH7에 관한 것으로서, 더욱 상세하게는 상기 시가톡신 생성 대장균 특이 박테리오파지 ECOH7을 포함하는 항균 조성물에 관한 것이다.The present invention relates to a novel cytotoxin-producing E. coli-specific bacteriophage ECOH7, and more particularly to an antimicrobial composition comprising the above-mentioned cytotoxin-producing E. coli-specific bacteriophage ECOH7.

시가톡신 생성 대장균(Shiga Toxin-producing E. coli, STEC)은 단백질 독소인 시가톡신(Shiga toxin)이라는 독소를 만들어내 질병을 유발한다. 상기 시가톡신 생성 대장균에는 시가 유사독소(Shiga-like toxin, SLT)인 베로독소(Verotoxin)을 생산하는 베로독소 생성 대장균(Verocytotoxic E. coli, VTEC) 및 장관출혈성 대장균(Enterohemorrhagic E. coli, EHEC)이 속한다.Shiga Toxin-producing E. coli (STEC) produces toxins called Shiga toxins, a protein toxin, that cause diseases. The cigar toxins produced E. coli is similar to Shiga toxin (Shiga-like toxin, SLT) of Vero Vero toxin producing E. coli produce toxins (Verotoxin) (Verocytotoxic E. coli, VTEC) and secretary hemorrhagic Escherichia coli (Enterohemorrhagic E. coli, EHEC) .

시가톡신 생성 대장균 중 장관출혈성 대장균은 베로독소(Verotoxin)를 생성하여 대장점막에 궤양을 유발하여 조직을 짓무르게 하고 출혈을 유발한다. 상기 장관출혈성 대장균은 혈청형에 따라 O26, O103, O104, O146 및 O157 등이 있으며, 대표적인 장관출혈성 대장균은 O157:H7이 있다. 장관출혈성 대장균 O157:H7에 감염된 개체의 치사율은 1,000명 당 6 내지 7명 정도로 낮은 편이나, 전염성이 강해 짧은 시간에 퍼지며, 소량의 균이 감염이 되더라도 병원성을 나타낼 수 있어 위해성은 높다고 할 수 있다. 장관출혈성 대장균 O157:H7 감염을 유발하는 원인식품의 경우 광범위하게 분포하기 때문에 환자와 보균자의 분변으로부터 직·간접으로 오염되는 식품이면 모두 원인식품이 될 수 있으며, 축산물, 육류 가공품, 햄, 치즈, 소시지, 채소샐러드, 분유, 두부, 음료수, 어패류, 도시락, 급식 등이 주요 원인식품이다.Shiga toxin-producing Escherichia coli produces verotoxin, which causes ulceration in the large intestine mucosa, causing irritation and bleeding. The intestinal hemorrhagic Escherichia coli has O26, O103, O104, O146, and O157 according to the serotype, and representative intestinal hemorrhagic Escherichia coli is O157: H7. The mortality rate of individuals infected with intestinal hemorrhagic Escherichia coli O157: H7 is as low as 6 to 7 per 1,000, but it is highly contagious and spreads in a short time, and even if a small amount of bacteria infects, the risk is high and the risk is high . Foods that cause intestinal hemorrhagic Escherichia coli O157: H7 infection are widely distributed, so any food contaminated directly or indirectly from the feces of patients and carriers may be the causative food and may be caused by livestock products, meat products, ham, cheese, Sausage, vegetable salad, powdered milk, tofu, beverage, seafood, lunch, lunch, etc. are the main causes of food.

상기 장관출혈성 대장균 O157:H7에 감염된 경우 주요 증상으로는 혈변, 발열, 구토, 설사와 복통이 가장 흔한 증상이며, 심각하게는 적혈구와 신장에 손상을 일으킬 수 있다. 대부분의 장관출혈성 대장균 O157:H7 감염은 7 내지 10일 정도면 회복되지만, 감염자 중 약 15%는 용혈성요독증후군(Hemolytic Uremic Syndrome, HUS)으로 발전하기도 하며, 대장균 감염에 민감한 사람들 즉, 임산부, 면역취약자, 영유아, 노인 등은 심각한 합병증에 걸릴 위험성이 높다.The major symptom of infection with the intestinal hemorrhagic Escherichia coli O157: H7 is the most common symptoms of stool, fever, vomiting, diarrhea and abdominal pain, and can seriously damage red blood cells and kidneys. Most intestinal hemorrhagic Escherichia coli O157: H7 infections are recovered by about 7 to 10 days, but about 15% of infections develop into Hemolytic Uremic Syndrome (HUS), and people who are susceptible to E. coli infection, such as pregnant women, Vulnerable children, infants, and elderly people are at high risk for serious complications.

한편, 박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지의 활용이 크게 주목을 받고 있으며, 자연 친화적 방식의 선호로 인하여 박테리오파지에 대한 관심은 어느 때보다 높다고 할 수 있다. 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다. 이러한 박테리오파지가 여러 세균성 질병을 대상으로 활발히 연구되고 있으며, 박테리오파지를 이용한 대장균 O157:H7의 제어는 안전한 식품에 의한 질병의 위험을 감소할 수 있는 방안이 될 수 있을 것으로 판단된다.On the other hand, bacteriophage refers to a bacterium-specific virus that infects a specific bacterium to inhibit and inhibit the growth of the infected bacterium. Bacteriophages have the ability to kill bacteria by infecting bacteria, multiplying inside bacterial cells, and destroying the cell walls of the host bacteria when the progeny bacteriophages come out of the bacteria after proliferation. The bacteriophage infection method of bacteriophage is highly specific, so the type of bacteriophage that can infect a specific bacterium is limited to some. That is, certain bacteriophages can infect only a specific category of bacteria, which causes certain bacteriophages to kill only certain bacteria and not other bacteria. Recently, the use of bacteriophage has attracted much attention as a countermeasure against bacterial diseases, and interest in bacteriophage is higher than ever due to preference of nature friendly method. Bacteriophage is attracting attention as an anti-bacterial agent again with the possibility of development as a substitute for existing antibiotics. These bacteriophages are actively studied for various bacterial diseases, and control of E. coli O157: H7 using bacteriophage may be a way to reduce the risk of disease caused by safe foods.

이에 본 발명자들은 박테리오파지를 기존 항생제의 대체 물질로 활용하기 위하여, 하수로부터 시가톡신 생성 대장균 특이 박테리오파지를 분리하였고, 분리된 박테리오파지의 형태적 및 유전학적 특성 분석을 통해 시가톡신 생성 대장균을 선택적으로 사멸시킬 수 있음을 확인함으로써 본 발명을 완성하게 되었다.Therefore, in order to utilize bacteriophage as a substitute for existing antibiotics, the present inventors have isolated a specific bacteriophage of ciguatoxin-producing E. coli from sewage and selectively analyzed the morphological and genetic characteristics of the isolated bacteriophage to kill the cytotoxin-producing E. coli The present invention has been completed.

따라서 본 발명의 목적은, 시가톡신 생성 대장균에 특이적인 사멸능을 갖고, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 ECOH7을 제공하는 것이다.Accordingly, an object of the present invention is to provide a bacteriophage ECOH7 having a killing activity specific to a cytotoxin-producing E. coli and consisting of the nucleotide sequence shown in SEQ ID NO: 1.

본 발명의 다른 목적은 상기 박테리오파지 ECOH7을 포함하는 항생용 조성물을 제공하는 것이다.Another object of the present invention is to provide an antibiotic composition comprising the bacteriophage ECOH7.

본 발명의 또 다른 목적은 상기 박테리오파지 ECOH7을 포함하는 사료 첨가제를 제공하는 것이다.It is a further object of the present invention to provide a feed additive comprising the bacteriophage ECOH7.

본 발명의 또 다른 목적은 상기 박테리오파지 ECOH7을 포함하는 소독제를 제공하는 것이다.It is still another object of the present invention to provide a disinfectant containing the bacteriophage ECOH7.

본 발명의 또 다른 목적은 상기 박테리오파지 ECOH7을 포함하는 세척제를 제공하는 것이다.It is still another object of the present invention to provide a cleaning agent comprising the bacteriophage ECOH7.

본 발명의 또 다른 목적은 상기 박테리오파지 ECOH7을 개체에 투여하는 단계를 포함하는 시가톡신 생성 대장균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.It is still another object of the present invention to provide a method for preventing or treating an infectious disease caused by a cytotoxin-producing E. coli comprising the step of administering the bacteriophage ECOH7 to an individual.

상기 목적을 달성하기 위하여, 본 발명은 시가톡신 생성 대장균에 특이적인 사멸능을 갖고, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 ECOH7을 제공한다.In order to accomplish the above object, the present invention provides bacteriophage ECOH7 having a killing activity specific to a cytotoxin-producing E. coli and comprising the nucleotide sequence shown in SEQ ID NO: 1.

또한 본 발명은 상기 박테리오파지 ECOH7을 포함하는 항생용 조성물을 제공한다.The present invention also provides an antibiotic composition comprising the bacteriophage ECOH7.

또한 본 발명은 상기 박테리오파지 ECOH7을 포함하는 사료 첨가제를 제공한다.The present invention also provides a feed additive comprising the bacteriophage ECOH7.

또한 본 발명은 상기 박테리오파지 ECOH7을 포함하는 소독제를 제공한다.The present invention also provides a disinfectant comprising the bacteriophage ECOH7.

또한 본 발명은 상기 박테리오파지 ECOH7을 포함하는 세척제를 제공한다.The present invention also provides a detergent comprising the bacteriophage ECOH7.

또한 본 발명은 상기 박테리오파지 ECOH7을 개체에 투여하는 단계를 포함하는 시가톡신 생성 대장균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.The present invention also provides a method for preventing or treating an infectious disease caused by a cytotoxin-producing E. coli comprising the step of administering the bacteriophage ECOH7 to an individual.

본 발명에 따른 박테리오파지 ECOH7은 시가톡신 생성 대장균에 대한 특이성이 매우 높고, 시가톡신 생성 대장균에 감염하여 시가톡신 생성 대장균 내에서 증식함으로써 용균 활성이 우수하며, 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 ECOH7은 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는바, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 분야에서 다양하게 활용될 수 있다.The bacteriophage ECOH7 according to the present invention has a very high specificity to the cytotoxin-producing E. coli and has an advantage of being excellent in lytic activity and strong against physicochemical stimulation by proliferation in the cigatotoxin-producing E. coli by infection with the cytotoxin-producing E. coli. In addition, since the bacteriophage ECOH7 of the present invention is not infected with any host other than bacteria such as human, animal, and plant, problems of antibiotic resistant bacteria caused by abuse of antibiotics, antibiotic residue in food, A composition for feed addition, a feed, a disinfectant or a detergent.

도 1은 시가톡신 생성 대장균 O157:H7에 대해 특이적인 박테리오파지의 스크리닝 결과를 나타낸 도이다.
도 2는 본 발명에 따른 박테리오파지 ECOH7의 시가톡신 생성 대장균 O157:H7에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 3은 본 발명에 따른 박테리오파지 ECOH7의 전체 유전자를 분석한 결과를 나타낸 도이다.
도 4는 전자 현미경을 통해 본 발명에 따른 박테리오파지 ECOH7의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 5는 SDS-PAGE를 통해 본 발명에 따른 박테리오파지 ECOH7의 단백질 패턴을 확인한 결과를 나타낸 도이다.
도 6은 본 발명에 따른 박테리오파지 ECOH7의 pH 안정성을 확인한 결과를 나타낸 도이다.
도 7은 본 발명에 따른 박테리오파지 ECOH7의 열 안정성을 확인한 결과를 나타낸 도이다.Brief Description of the Drawings Fig. 1 is a diagram showing a screening result of bacteriophage specific for the cytotoxin-producing E. coli O157: H7.
FIG. 2 is a graph showing the results of confirming the activity of the bacteriophage ECOH7 according to the present invention for the dissociation of the cyanotic toxin-producing E. coli O157: H7.
FIG. 3 is a graph showing the results of analysis of whole genes of bacteriophage ECOH7 according to the present invention.
FIG. 4 is a graph showing the morphological characteristics of the bacteriophage ECOH7 according to the present invention through an electron microscope.
FIG. 5 is a graph showing the result of confirming the protein pattern of the bacteriophage ECOH7 according to the present invention through SDS-PAGE. FIG.
FIG. 6 is a graph showing the pH stability of the bacteriophage ECOH7 according to the present invention.
7 is a graph showing the results of checking the thermal stability of the bacteriophage ECOH7 according to the present invention.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 시가톡신 생성 대장균(Shiga Toxin-producing Escherichia coli, STEC)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 ECOH7을 제공한다.The present invention relates to a method for producing Shiga toxin-producing Escherichia Bacteriophage ECOH7 comprising the nucleotide sequence shown in SEQ ID NO: 1, which has a killing activity specific to E. coli , STEC.

본 발명에서, 용어 “시가톡신 생성 대장균(Shiga Toxin-producing Escherichia coli, STEC)”은 시가톡신(Shiga toxin)을 생성하거나, 시가 유사 독소(Shiga-like toxin, SLT)인 베로독소(Verotoxin)를 생성하는 대장균을 의미한다.In the present invention, the term " Shiga Toxin-producing Escherichia coli , STEC) "refers to Escherichia coli that produces Shiga toxin or produces Verotoxin, a Shiga-like toxin (SLT).

본 발명의 시가톡신 생성 대장균은 장관출혈성 대장균(Enterohaemorrhagic E. coli, EHEC), 장병원성 대장균(Enteropathogenic E. coli, EPEC) 및 베로독소 생성 대장균(Verocytotoxic E. coli, VTEC)으로 구성된 군으로부터 선택된 1종 이상일 수 있으며, 바람직하게는 장관출혈성 대장균 O157:H7일 수 있다.The cytotoxin-producing Escherichia coli of the present invention can be used for enterohaemorrhagic E. coli , EHEC), Enteropathogenic E. coli (EPEC), and Verocytotoxic E. coli (VTEC), and preferably can be at least one selected from the group consisting of intestinal hemorrhagic Escherichia coli O157: H7 .

본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.In the present invention, the term bacteriophage refers to a bacterium-specific virus that infects a specific bacterium and inhibits and inhibits the growth of the bacterium, and includes a single or double-stranded DNA or RNA as a genetic material.

본 발명의 박테리오파지 ECOH7은 시가톡신 생성 대장균(Shiga toxin producing E. coli, STEC)에 대해 용균활성이 탁월하다. The bacteriophage ECOH7 of the present invention is excellent in lytic activity against Shiga toxin producing E. coli (STEC).

본 발명의 박테리오파지 ECOH7은 178 내지 189 nm의 크기로, 정이십면체의 머리를 가지며 수축형 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속함을 확인하였으며, 단백질 패턴 분석 결과 90, 80, 55, 40, 21, 17 및 14 kDa 크기의 주요 단백질을 포함한다. 또한 상기 박테리오파지 ECOH7은 열 및 pH에 대한 안정성이 우수하고, 세균 사멸능이 탁월하다는 특성을 가지고 있다.The bacteriophage ECOH7 of the present invention was found to belong to Myoviridae having a size of 178 to 189 nm and having a truncated sagittal head and a contraction tail, and the protein pattern analysis revealed that 90, 80, 55, 40, 21, 17 and 14 kDa major proteins. The bacteriophage ECOH7 has excellent stability against heat and pH, and has an excellent bactericidal activity.

본 발명의 박테리오파지 ECOH7은 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 ECOH7은 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.The bacteriophage ECOH7 of the present invention may include the nucleotide sequence shown in SEQ ID NO: 1 as whole or part of the entire gene. In addition, the bacteriophage ECOH7 of the present invention may comprise the nucleotide sequence shown in SEQ ID NO: 1 and the functional equivalent of the nucleotide sequence. The functional equivalent means at least 70% or more, preferably 80% or more, more preferably 90% or more, still more preferably 95% or more of the nucleotide sequence shown in SEQ ID NO: 1 as a result of modification or substitution of the nucleotide sequence. Or more and having substantially the same physiological activity as the nucleotide sequence shown in SEQ ID NO: 1.

상기와 같은 시가톡신 생성 대장균 특이적 용균활성, 내산성, 내염기성 및 내열성은 본 발명의 박테리오파지 ECOH7을 시가톡신 생성 대장균에 의한 감염성 질병의 예방 및 치료용 조성물, 및 상기 박테리오파지 ECOH7을 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용이 가능하게 한다.The above-mentioned ciguatoxin-producing Escherichia coli-specific lytic activity, acid resistance, basic basicity and heat resistance can be obtained by a composition for preventing and treating infectious diseases caused by cytotoxin-producing Escherichia coli of the bacteriophage ECOH7 of the present invention and a composition containing the bacteriophage ECOH7 When applied to various products, it is possible to apply various temperature and pH ranges.

본 발명의 박테리오파지 ECOH7은 하수처리장 생물반응조, 도계장 및 양계농장 부근의 하수로부터 시료를 채취하여 분리한 박테리오파지로서, 박테리오파지 ECOH7로 명명하고 2017년 9월 13일에 한국미생물보존센터에 기탁번호 KFCC11732P로 기탁하였다.The bacteriophage ECOH7 of the present invention is a bacteriophage isolated from a sewage treatment plant bioreactor, a slaughterhouse and a poultry farm by collecting a sample, and named as bacteriophage ECOH7 and deposited on September 13, 2017 with the deposit number KFCC11732P Respectively.

또한, 본 발명은 상기 박테리오파지 ECOH7을 포함하는, 항생용 조성물을 제공한다. In addition, the present invention provides an antibiotic composition comprising the bacteriophage ECOH7.

본 발명에서, 용어 항생용 조성물은 약제 형태로 동물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다. In the present invention, the term antibiotic composition refers to a preparation which can be provided to an animal in the form of a drug to kill bacteria, and is collectively referred to as an antiseptic, a bactericide, an antibiotic and an antibacterial agent.

본 발명의 박테리오파지 ECOH7은 기존 항생제에 비하여 시가톡신 생성 대장균에 대한 특이성이 매우 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.Since the bacteriophage ECOH7 of the present invention is highly specific for the ciguatoxin-producing E. coli compared to the existing antibiotics, it does not kill useful bacteria, can kill only specific pathogens, and does not induce drug resistance or resistance, Can be used as a new antibiotic with a long life cycling.

또한, 본 발명은 상기 박테리오파지 ECOH7을 포함하는, 사료 첨가용 조성물을 제공한다.In addition, the present invention provides a composition for feed addition comprising the bacteriophage ECOH7.

양돈, 축산, 수산업, 반려동물 산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 동물에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞여 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 ECOH7을 이용할 수 있다.Feed antibiotics used in swine, livestock, fisheries and companion animal industries are used for the prevention of diseases. Antibiotic administration for prevention is problematic because it increases the possibility of resistant bacteria and antibiotics remaining in animals can be transmitted to humans . If antibiotics are absorbed into the body through meat, they may cause antibiotic resistance and spread the disease. In addition, since there are many kinds of antibiotics mixed with feeds and there is a problem that the probability of occurrence of multidrug-resistant bacteria increases, it is possible to solve the problem caused by the use of existing antibiotics while being more environmentally friendly, and as a new feed additive antibiotic substance, the bacteriophage ECOH7 Can be used.

또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조 상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량%, 바람직하게는 0.1 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.In addition, the present invention can provide a feed comprising the feed composition, and the feed of the present invention can be prepared by separately preparing bacteriophage as a feed additive and mixing the feed into the feed, . The bacteriophage in the feed of the present invention may be in a liquid or dry state, preferably in the form of a dried powder. Drying methods include, but are not limited to, air drying, natural drying, spray drying and freeze drying. The bacteriophage of the present invention may be mixed in powder form in a composition ratio of 0.05 to 10% by weight, preferably 0.1 to 2% by weight of the feed weight. In addition to the bacteriophages of the present invention, the feed may further include conventional additives that can enhance the preservability of the feed.

본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis), 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 동물의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae) 및 사카로미세스 세레비지에(Saccharomyces cerevisiae)로 구성된 군으로부터 선택될 수 있다.In the composition for feed addition of the present invention, microorganisms other than non-pathogenic microorganisms may be further added. Examples of microorganisms that can be added include Bacillus subtilis capable of producing proteolytic enzymes, lipolytic enzymes and glycosyltransferases, Lactobacillus strains having physiological activity and organic degradation ability under anaerobic conditions such as those above, sp.), increases the weight of the animal (Aspergillus in Aspergillus duck, showing the effect of increasing the milk production of the milk to increase the absorption of digested food party oryzae , and Saccharomyces cerevisiae .

본 발명의 박테리오파지 ECOH7을 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.Examples of the feedstuffs containing bacteriophage ECOH7 of the present invention include cereals, rootstocks, food processing buses, algae, fibers, pharmaceuticals, and oils, fats and oils, pastes, But are not limited to, proteins, mineralogy, oils, mineralogy, unicellular proteins, zooplankton, and remnants.

본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.The composition for feed addition of the present invention may contain a binder, an emulsifying agent, a preservative, etc. added to prevent deterioration of quality. An amino acid, a vitamin, an enzyme, a probiotic, a flavoring agent, A nitrogen compound, a silicate, a buffer, a coloring agent, an extracting agent, and an oligosaccharide. In addition, a feed mixture may be further included.

또한, 본 발명은 상기 박테리오파지 ECOH7을 포함하는, 소독제를 제공한다.The present invention also provides a disinfectant comprising the bacteriophage ECOH7.

시가톡신 생성 대장균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 ECOH7을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.The disinfectant containing the bacteriophage ECOH7 of the present invention having a specific killing activity against the cytotoxin-producing E. coli can be effectively used as a disinfectant for hospitals and healthcare to prevent hospital infection and can be used as a disinfectant for general living disinfectants, foods, Disinfection of various kinds of growing equipment such as poultry houses, housing buildings such as pens, shaft bodies, water, litter, stairs, transportation vehicles, tableware, and the like.

또한, 본 발명은 상기 박테리오파지 ECOH7을 포함하는, 세척제를 제공한다.The present invention also provides a detergent comprising the bacteriophage ECOH7.

본 발명의 박테리오파지 ECOH7은 시가톡신 생성 대장균에 특이적 사멸능을 가지므로, 상기 시가톡신 생성 대장균에 노출되었거나 노출될 가능성이 있는 동물의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.Since the bacteriophage ECOH7 of the present invention has a specific killing effect on the cytotoxin-producing E. coli, it can also be used for washing the surface of skin or parts of the body of an animal which is exposed or exposed to the above-mentioned cytotoxin-producing E. coli .

또한, 본 발명은 상기 박테리오파지 ECOH7을 개체에 투여하는 단계를 포함하는, 시가톡신 생성 대장균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.The present invention also provides a method for preventing or treating an infectious disease caused by a cytotoxin-producing E. coli, which comprises the step of administering the bacteriophage ECOH7 to an individual.

본 발명의 방법은 시가톡신 생성 대장균에 의한 감염성 질병, 예컨대 세균성 식중독, 출혈성 대장염, 용혈성 요독증후군 또는 혈전성 혈소판 감소성 자반증이 발생한 개체에 박테리오파지 ECOH7을 투여하는 단계를 포함한다. The method of the present invention comprises the step of administering bacteriophage ECOH7 to an infectious disease caused by cytotoxin-producing E. coli, such as bacterial food poisoning, hemorrhagic colitis, hemolytic uremic syndrome or thrombotic thrombocytopenic purpura.

본 발명에서, 용어 "개체"는 인간을 포함한 동물, 바람직하게는 인간, 가축류 또는 반려동물을 말하며, 이에 제한되는 것은 아니다.In the present invention, the term "individual" refers to an animal, including a human, preferably a human, livestock or companion animal, but is not limited thereto.

상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 인간과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 조류, 어류, 곤충류, 포유류를 모두 포함하는 개념이며, 상기 가축류는 돼지, 소, 닭, 말, 오리 및 개로 구성된 군에서 선택될 수 있으나, 이에 제한되는 것은 아니다.The term "livestock" is a concept that refers to useful animals that are refined and improved by humans to co-live with humans, including birds, fishes, insects, and mammals. The livestock are pigs, , Horse, duck, and dog, but is not limited thereto.

본 발명의 방법에서 상기 박테리오파지 ECOH7 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 개체의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.In the method of the present invention, the bacteriophage ECOH7 or the composition may be administered to an animal in the form of a pharmaceutical preparation, or may be administered through a method of ingesting the bacteriophage ECOH7 or a mixture thereof in an animal feed or drinking water.

본 발명의 방법에서 상기 박테리오파지 ECOH7 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.In the method of the present invention, the administration route of the bacteriophage ECOH7 or composition can be administered through various routes of oral or parenteral administration, as long as it can reach the target tissues, and specifically, oral, rectal, topical, intravenous, Intravenous, intramuscular, intraarterial, transdermal, intranasal, inhalation, and the like.

본 발명의 방법에 투여되는 상기 박테리오파지 ECOH7의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.It will be apparent to those skilled in the art that the appropriate total daily dose of the bacteriophage ECOH7 administered to the method of the present invention may be determined by treatment within the scope of sound medical judgment. The specific therapeutically effective amount for a particular individual will depend upon the type and extent of the response to be achieved, the age, weight, general health, sex and diet of the patient, the time of administration, the route of administration and the fraction of the route of administration, It is preferable to apply differently depending on various factors including drugs used or co-used and similar factors well known in the medical field.

본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.Terms not otherwise defined herein have meanings as commonly used in the art to which the present invention belongs.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

실시예Example 1.  One. 신규한New 박테리오파지의 분리 및 배양 Isolation and culture of bacteriophage

1-1. 박테리오파지의 스크리닝 및 단일 박테리오파지의 분리1-1. Screening of bacteriophages and isolation of single bacteriophages

대장균 O157:H7에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 반점 용균반 분석을 수행하였다. 시료는 박테리오파지가 존재할 것으로 예상되는 하수처리장 생물반응조, 도계장 및 양계농장 부근의 하수로부터 시료를 채취하였으며, 박테리오파지 분리에 사용된 대장균 O157:H7은 (주)옵티팜에서 분리 및 동정한 균주를 사용하였다.Bacterial plaque assay was performed to screen and isolate E. coli O157: H7 specific bacteriophages. Samples were collected from sewage treatment plant bioreactors, slaughterhouses and poultry farms where bacteriophages are expected to be present, and E. coli O157: H7 used for bacteriophage isolation was isolated and identified from Opti Pharm Co., Ltd. .

구체적으로, 채취한 시료를 균질화 한 후 대장균 O157:H7과 함께 35℃에서 하룻밤 동안 진탕배양 하였다. 상기 배양액을 4,000 rpm으로 20분간 원심분리한 후, 상등액을 공극(pore) 크기가 0.2 μm인 필터를 이용하여 여과하여 시료 여과액을 얻었다. 대장균 O157:H7를 용균할 수 있는 박테리오파지가 존재하는지 스크리닝을 수행하였고, 그 결과는 도 1에 나타내었다.Specifically, the collected sample was homogenized and incubated with E. coli O157: H7 at 35 占 폚 overnight with shaking. The culture was centrifuged at 4,000 rpm for 20 minutes, and the supernatant was filtered using a filter having a pore size of 0.2 μm to obtain a sample filtrate. Screening was carried out to confirm the presence of bacteriophage capable of lysing E. coli O157: H7, and the results are shown in Fig.

전배양된 대장균 O157:H7 150 μL을 취해서 멸균된 튜브에 넣고, 3 mL의 LB 탑 아가[LB 배지(트립톤 10 g/L, 효모 추출물(yeast extract) 5 g/L, NaCl 10 g/L) + 0.6% 아가]를 첨가하여 잘 혼합한 후 미리 만들어 둔 LB 아가 플레이트에 중층하였다. 실온에서 30분간 방치한 후 준비된 샘플 여과액을 각각 10 uL씩 점적한 후 실온에서 30분 동안 방치하였고, 이를 35℃에서 하룻밤 동안 배양한 후 박테리오파지의 유무를 확인하였다.150 μL of pre-cultured E. coli O157: H7 was placed in a sterilized tube and 3 mL of LB topa agar [LB medium (10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L of NaCl ) + 0.6% agar] was added thereto, and the mixture was well mixed and then layered on the previously prepared LB agar plate. After incubation at room temperature for 30 minutes, 10 μL of the prepared sample filtrate was added to each well, and the mixture was allowed to stand at room temperature for 30 minutes. After incubation at 35 ° C. overnight, bacteriophage was observed.

박테리오파지의 존재를 확인한 시료로부터 박테리오파지를 순수 분리하기 위해서 소프트 아가 오버레이(soft agar overlay)법을 이용하였다. 박테리오파지가 존재하는 것으로 확인된 시료를 식염수로 적절히 희석하여 각 희석단의 시료 희석액 100 μL를 취하고, 대상 세균(대장균 O157:H7) 배양액 150 μL에 혼합한 후 0.6% LB 탑 아가 3 mL을 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% LB 아가 플레이트에 중층하여 방치한 후 35℃에서 24시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후, 이를 선택하여 400 μL의 SM 완충액(NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-HCl(pH7.5) 50 mL)에 현탁하고, 실온에서 4시간 동안 방치하였다. 상기 박테리오파지액 100 μL를 0.6% LB 탑 아가가 포함된 세균 배양액 500 μL와 혼합한 뒤 150 mm 지름의 LB 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, LB 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간 동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 4,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.45 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 2에 나타내었다.A soft agar overlay method was used to isolate the bacteriophage from the sample which confirmed the existence of the bacteriophage. A sample of which the bacteriophage was confirmed to be present was diluted appropriately with saline, 100 μL of sample dilution at each dilution stage was taken, mixed with 150 μL of the bacterial culture (E. coli O157: H7), and 3 mL of 0.6% LB topa After well mixing, the mixture was allowed to stand in a 2% LB agar plate which had been previously prepared, and then incubated at 35 ° C for 24 hours. After culturing, the plate was selected, and 400 μL of SM buffer solution (NaCl 5.8 g / L; MgSO 4 7H 2 O 2 g / L; 1M Tris-HCl (pH 7.5) 50 mL) was added to each plate Suspended and allowed to stand at room temperature for 4 hours. 100 .mu.L of the bacteriophage solution was mixed with 500 .mu.L of a bacterial culture solution containing 0.6% LB top agar, and the mixture was divided into 150-mm-diameter LB plates and cultured at 35.degree. After completion of the incubation, 15 mL of SM buffer was added to the LB plate, and the bacteriophage solution was recovered while slowly stirring at room temperature for 4 hours. Then, the supernatant was centrifuged at 4,000 rpm for 20 minutes to filter out the supernatant. The solution was recovered. FIG. 2 shows the result of checking the thus obtained final bacteria bacteriophage.

도 2에 나타낸 바와 같이, 2 내지 3 mm의 크기를 가지는 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 대장균 O157:H7에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.As shown in Fig. 2, a bacteriophage forming a fungus having a size of 2 to 3 mm was confirmed, and it was confirmed that the bacteriophage was a bacteriophage having a solubility specific to E. coli O157: H7.

1-2. 박테리오파지의 대량 배양 및 정제1-2. Bulk culture and purification of bacteriophages

상기 실시예 1-1에서 확인한 대장균 O157:H7 특이적 박테리오파지를 대량 배양하기 위하여, 실시예 1-1에서 회수한 최종 박테리오파지 배양액을 1.0x107 PFU/mL로 희석한 후 전배양된 대장균 O157:H7 1.0x109 CFU/mL과 혼합하여 MOI(Multiplicity of infection)가 0.01이 되도록 35℃에서 120 rpm으로 9시간 동안 배양하였다. 배양이 종료된 후 배양액 전량을 6,000 rpm으로 30분간 원심분리하여 상등액을 수득하였고, 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다. 분리된 박테리오파지를 "박테리오파지 ECOH7(Bacteriophage ECOH7)"로 명명하고, 2017년 9월 13일 한국미생물보존센터(Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하여 기탁번호 KFCC11732P를 부여받았다.In order to mass-culture the E. coli O157: H7 specific bacteriophage identified in Example 1-1, the final bacteriophage culture recovered in Example 1-1 was diluted to 1.0 x 10 7 PFU / mL, and the pre-cultured E. coli O157: H7 1.0 × 10 9 CFU / mL and cultured at 35 ° C. and 120 rpm for 9 hours so that the MOI (multiplicity of infection) was 0.01. After the culture was completed, the whole culture was centrifuged at 6,000 rpm for 30 minutes to obtain a supernatant, which was then filtered with a 0.2 μm filter to recover the final bacteriophage culture. The isolated bacteriophage was named "Bacteriophage ECOH7" and deposited with the Korean Culture Center of Microorganisms (Seoul, Korea) on September 13, 2017, and received the deposit number KFCC11732P.

실시예Example 2. 박테리오파지  2. Bacteriophage ECOH7의Of ECOH7 전체 유전자 분석 Whole gene analysis

박테리오파지 ECOH7의 전체 유전자를 분석하기 위해, 박테리오파지 ECOH7 배양액에 20 mM EDTA, 50 μg/mL의 프로테나아제 K, 0.5%(w/v) SDS를 가한 후, 50℃에서 1시간 동안 정치하였다. 동량의 페놀-클로로포름-이소프로필 알코올(25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액 회수하였다. 회수된 상등액을 동일 부피의 페놀-클로로포름-이소프로필 알코올(25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액 회수하고, 상기 상등액에 3M 소듐 아세테이트를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 1시간 동안 정치하였다. 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리하여 침전물 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA 펠렛을 회수하였다. 회수한 펠렛을 최종 부피가 50 μL이 되도록 TE 완충액(Tris-EDTA, pH8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 PacBioRSII를 이용하여 유전자 시퀀싱을 시행하였다(마크로젠, 한국). 시퀀싱 결과로 얻은 박테리오파지 ECOH7의 유전자 맵은 도 3에 나타내었다.To analyze the entire gene of bacteriophage ECOH7, 20 mM EDTA, 50 μg / mL proteinase K and 0.5% (w / v) SDS were added to the bacteriophage ECOH7 culture medium and left at 50 ° C. for 1 hour. The mixture was mixed with an equal amount of phenol-chloroform-isopropyl alcohol (25: 24: 1) and centrifuged at 12,000 rpm for 10 minutes at room temperature to recover the supernatant. The recovered supernatant was mixed with an equal volume of phenol-chloroform-isopropyl alcohol (25: 24: 1) and centrifuged at 12,000 rpm for 10 minutes at room temperature. The supernatant was recovered and 3M sodium acetate was added to the supernatant in a total volume of 10 % (v / v). The mixture was mixed with 2 volumes of cold 95% ethanol, and the mixture was allowed to stand at -20 DEG C for 1 hour. After the reaction was completed, the precipitate was collected by centrifugation at 12,000 rpm for 10 minutes at 0 ° C., washed twice with cold 70% ethanol, and then the ethanol was completely removed to recover the DNA pellet. The recovered pellet was dissolved in TE buffer (Tris-EDTA, pH 8.0) to a final volume of 50 μL, and the DNA concentration was measured. Gene sequencing was performed using PacBioRSII for whole gene analysis (Macrogen, Korea). The gene map of bacteriophage ECOH7 obtained as a result of sequencing is shown in Fig.

도 3에 나타낸 바와 같이, 박테리오파지 ECOH7의 전체 유전자의 크기는 85,260 bp이며, GC함량이 38.9%, 전체 ORFs의 수는 117개임을 확인하였다. 분석한 박테리오파지 ECOH7의 전체 유전자 서열을 서열번호 1로 나타내었다. As shown in FIG. 3, it was confirmed that the size of the whole gene of bacteriophage ECOH7 was 85,260 bp, the GC content was 38.9%, and the total ORFs were 117. The entire gene sequence of the analyzed bacteriophage ECOH7 is shown in SEQ ID NO: 1.

실시예Example 3. 박테리오파지  3. Bacteriophage ECOH7의Of ECOH7 형태학적 특성 Morphological characteristics

박테리오파지 ECOH7의 형태학적 특성을 알아보기 위해, 박테리오파지 ECOH7의 순수배양액을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 탄소-코팅 운모 플레이트(carbon-coated mica plate(ca. 2.5 x 2.5 mm))에 적가한 후, 10분간 적응시킨 후 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30 내지 60초 동안 염색하고 건조시킨 후, 투과전자현미경(Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 검경하였으며, 그 결과를 도 4에 나타내었다.To determine the morphological characteristics of bacteriophage ECOH7, the pure culture medium of bacteriophage ECOH7 was diluted in 0.01% gelatin solution and fixed with 2.5% glutaraldehyde solution. This was applied to a carbon-coated mica plate (ca. 2.5 x 2.5 mm), followed by a 10-minute adaptation and washing with sterile distilled water. The resultant was stained with 2% Uranyl acetate for 30 to 60 seconds, and dried using a transmission electron microscope (Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 400,000), and the results are shown in FIG.

도 4에 나타낸 바와 같이, 박테리오파지 ECOH7은 전체 길이 178 내지 189 nm의 크기로, 정이십면체의 머리를 가지며 수축성 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속함을 확인하였다.As shown in Fig. 4, the bacteriophage ECOH7 was found to belong to the Myoviridae, which is 178 to 189 nm in total length, and has a truncated dichroic head and a contractile tail.

실시예Example 4. 박테리오파지  4. Bacteriophage ECOH7의Of ECOH7 단백질 패턴 분석 Protein pattern analysis

박테리오파지 ECOH7의 단백질 패턴을 분석하기 위해, 1.0x1010 PFU/mL로 준비된 박테리오파지 ECOH7 40 μL를 10 μL의 5X 로딩 완충액과 혼합하고, 10분간 끓인 다음 12.5% SDS-PAGE를 수행하였다. SDS-PAGE 수행 후 단백질 전기영동 결과를 도 5에 나타내었다.To analyze the protein pattern of bacteriophage ECOH7, 40 μL of bacteriophage ECOH7 prepared with 1.0 × 10 10 PFU / mL was mixed with 10 μL of 5X loading buffer, boiled for 10 minutes, and then subjected to 12.5% SDS-PAGE. The results of SDS-PAGE and protein electrophoresis are shown in FIG.

도 5에 나타낸 바와 같이, 박테리오파지 ECOH7은 90, 80, 55, 40, 21, 17 및 14 kDa 크기의 주요 단백질을 가지는 것을 확인하였다.As shown in FIG. 5, bacteriophage ECOH7 has 90, 80, 55, 40, 21, 17 and 14 kDa major proteins.

실시예Example 5. 박테리오파지  5. Bacteriophage ECOH7의Of ECOH7 다양한 세균에 대한 감염성 조사 Investigation of infectivity against various bacteria

박테리오파지 ECOH7이 다양한 세균에 대하여 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양받은 균주를 대상으로 소프트 아가 오버레이법을 통해서 감염성을 확인하였다. 그 결과를 표 1에 나타내었다.In order to confirm that bacteriophage ECOH7 exhibits lytic activity against various bacteria, the strains isolated from OptiParm Co., Ltd. and purchased from the microorganism resource center were examined for infectivity through soft agar overlay method. The results are shown in Table 1.

Figure pat00001
Figure pat00001

표 1에 나타낸 바와 같이, 대장균 및 다른 미생물을 대상으로 감수성 검사를 진행한 결과, 박테리오파지 ECOH7은 장관출혈성 대장균인 O157:H7 및 시가톡신 생성 대장균(STEC)에 대해서 용균활성을 나타내는 것을 확인하였다. 그러나, 비병원성 대장균, Salmonella Typhimurium, Bacillus cereus, Listeria monocytogenes, Lactobacillus plantarum 등에는 용균활성이 없는 것을 확인하였다. As shown in Table 1, the susceptibility test of E. coli and other microorganisms showed that bacteriophage ECOH7 exhibited lytic activity against O157: H7 and E. coli producing stigma E. coli (STEC), which are intestinal hemorrhagic Escherichia coli. However, non-pathogenic Escherichia coli, Salmonella Typhimurium , Bacillus cereus , Listeria monocytogenes, Lactobacillus plantarum And the like.

따라서, 본 발명의 박테리오파지 ECOH7은 시가톡신 대장균(STEC)에 특이적으로 용균활성을 나타내는 박테리오파지임을 확인하였다.Therefore, it was confirmed that the bacteriophage ECOH7 of the present invention is a bacteriophage exhibiting a lytic activity specifically against the cytotoxin Escherichia coli (STEC).

실시예Example 6. 박테리오파지  6. Bacteriophage ECOH7의Of ECOH7 pH 안정성 조사 pH stability investigation

박테리오파지 ECOH7의 pH 안정성을 확인하기 위하여, 다양한 pH 범위 (pH2, 3, 4, 5, 6, 7, 8, 9, 10, 11)에서 박테리오파지의 수를 측정하였다. 소듐 아세테이트 완충액, 소듐 포스페이트 완충액, Tris-HCl을 이용하여 각각의 pH에 맞게 조제한 후 1 mL로 분주하여 준비하였다. 1.0x108 PFU/mL로 준비한 박테리오파지 ECOH7 배양액 10 μL을 상기 용액에 첨가하여 2시간 동안 상온에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 타이터(titer) 측정을 수행한 결과를 도 6에 나타내었다.The number of bacteriophages was measured in various pH ranges (pH 2, 3, 4, 5, 6, 7, 8, 9, 10, 11) in order to confirm the pH stability of bacteriophage ECOH7. Sodium acetate buffer, sodium phosphate buffer, and Tris-HCl were added to each pH-adjusted solution, and the mixture was divided into 1 mL. 10 μL of the bacteriophage ECOH7 culture prepared in 1.0 × 10 8 PFU / mL was added to the above solution and left at room temperature for 2 hours. The reaction solution was allowed to stand, and the reaction solution was diluted stepwise, and a titer measurement was performed by soft agar overlay method. The result is shown in FIG.

도 6에 나타낸 바와 같이, 박테리오파지 ECOH7은 pH 3 내지 11의 범위에서 2시간 동안 안정한 것을 확인하였다. 따라서, 박테리오파지 ECOH7은 pH 안정성이 우수한 박테리오파지임을 알 수 있다.As shown in Fig. 6, it was confirmed that bacteriophage ECOH7 was stable for 2 hours in the range of pH 3 to 11. Therefore, it can be seen that bacteriophage ECOH7 is a bacteriophage having excellent pH stability.

실시예Example 7. 박테리오파지  7. Bacteriophage ECOH7의Of ECOH7 열 안정성 조사 Thermal stability investigation

박테리오파지 ECOH7의 열 안정성을 확인하기 위하여, 다양한 온도 범위 (40℃, 50℃, 60℃, 70℃)에서 박테리오파지의 수를 측정하였다. 박테리오파지 ECOH7 배양액(1.0x108 PFU/mL) 5 mL을 멸균된 튜브에 분주한 후 박테리오파지를 첨가하여 2시간 동안 각 온도에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 타이터 측정을 수행한 결과를 도 7에 나타내었다.In order to confirm the thermal stability of bacteriophage ECOH7, the number of bacteriophages was measured in various temperature ranges (40 ° C, 50 ° C, 60 ° C, 70 ° C). 5 mL of bacteriophage ECOH7 culture (1.0 x 10 8 PFU / mL) was dispensed into sterile tubes and bacteriophage was added and left at each temperature for 2 hours. After allowing to stand, each reaction solution was diluted stepwise, and the result of performing a tester measurement by soft agar overlay method is shown in FIG.

도 7에 나타낸 바와 같이, 박테리오파지 ECOH7은 40 내지 50℃의 범위에서 60분까지 안정한 것을 확인하였다. 따라서 상기 박테리오파지 ECOH7은 내열성이 우수한 박테리오파지임을 알 수 있다.As shown in Fig. 7, it was confirmed that the bacteriophage ECOH7 was stable up to 60 minutes in the range of 40 to 50 占 폚. Therefore, it can be seen that the bacteriophage ECOH7 is a bacteriophage having excellent heat resistance.

실시예Example 8. 박테리오파지  8. Bacteriophage ECOH7의Of ECOH7 세균  Germ 사멸능Destructiveness 조사 Research

박테리오파지 ECOH7의 세균 사멸능을 확인하기 위하여, 배양 시간에 따른 세균의 배양 상태를 조사하였다. 구체적으로, 600 nm에서 흡광도가 0.5인 세균 배양액(대장균 O157:H7) 10 mL을 준비하였다. 상기 세균 배양액에 1.0x1010 PFU/mL의 박테리오파지 ECOH7 배양액 1 mL을 첨가하여 MOI(Multiplicity of infection)가 10이 되도록 접종하였다(실험군 1). 박테리오파지 ECOH7이 접종된 세균 배양액을 3 시간 동안 배양하였으며, 1 시간 단위로 파장 600 nm에서의 흡광도를 측정하였다.To determine the bactericidal activity of bacteriophage ECOH7, the culture conditions of bacteria were investigated according to the incubation time. Specifically, 10 mL of a bacterial culture solution (E. coli O157: H7) having an absorbance of 0.5 at 600 nm was prepared. 1 ml of 1.0 × 10 10 PFU / ml bacteriophage ECOH7 culture was added to the bacterial culture to inoculate MOI (multiplicity of infection) of 10 (experimental group 1). Bacterial culture inoculated with bacteriophage ECOH7 was incubated for 3 hours and the absorbance at 600 nm was measured in 1 hour.

실험군 2는 실험군 1과 동일하게 세균 사멸능을 조사하되, 세균 배양액에 1.0x109 PFU/mL의 박테리오파지 ECOH7 배양액 1 mL을 첨가하여 MOI가 1이 되도록 접종하였다. 대조군은 박테리오파지 ECOH7 배양액을 첨가하지 않은 세균 배양액을 사용하였다. 박테리오파지 ECOH7의 세균 사멸능 조사 결과는 표 2에 나타내었다.Experimental group 2 was inoculated with 1 × 10 9 PFU / mL bacteriophage ECOH7 medium (1 mL) to the culture medium. As a control group, a culture solution of bacteriophage ECOH7 was used. The results of the bacteriocidal activity of bacteriophage ECOH7 are shown in Table 2.

Figure pat00002
Figure pat00002

표 2에 나타낸 바와 같이, 박테리오파지 ECOH7을 첨가한 실험군 1 및 2는 대조군보다 흡광도가 낮게 측정되었다. 상기 결과는 대장균 O157:H7에 대한 박테리오파지 ECOH7의 사멸능이 우수함을 의미한다.As shown in Table 2, the experimental groups 1 and 2 to which bacteriophage ECOH7 was added had lower absorbance than the control group. This result means that the killing effect of bacteriophage ECOH7 on E. coli O157: H7 is excellent.

종합적으로 본 발명자들은 박테리오파지 ECOH7가 시가톡신 생성 대장균에 대한 특이성이 매우 높고, 시가톡신 생성 대장균에 감염하여 시가톡신 생성 대장균 내에서 증식함으로써 용균 활성이 우수하며, 물리화학적 자극에 강하다는 장점을 가지고 있음을 확인하였다. 또한 본 발명의 박테리오파지 ECOH7은 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서 본 발명의 박테리오파지 ECOH7은 항생용 조성물, 사료첨가용 조성물, 사료, 소독제 또는 세척제 분야에서 다양하게 활용될 수 있다.In general, the present inventors have the advantage that the bacteriophage ECOH7 is highly specific for the cytotoxin-producing Escherichia coli, has an excellent lytic activity by being infected with the cytotoxin-producing Escherichia coli and proliferating in the cytotoxin-producing Escherichia coli, and is resistant to physicochemical stimulation Respectively. In addition, the bacteriophage ECOH7 of the present invention is not infected with any host other than bacteria such as human, animal, and plant. Therefore, it is possible to solve problems of antibiotic resistant bacteria caused by misuse of antibiotics, residual antibiotics in food, have. Therefore, the bacteriophage ECOH7 of the present invention can be used in various fields in antibiotic composition, composition for feed addition, feed, disinfectant or detergent.

이하, 제제예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 제제예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 제제예에 의해 제한되는 것으로 해석되지 않는다.Hereinafter, the present invention will be described in more detail with reference to formulation examples. The formulation examples are for illustrating the present invention only, and the scope of the present invention is not construed as being limited by formulation examples.

제제예Formulation example 1. 사료 첨가용 조성물 1. Composition for Feed Additive

박테리오파지 ECOH7 1x107 PFU/mLBacteriophage ECOH7 1x10 7 PFU / mL

비타민 믹스 100 mgVitamin mix 100 mg

미네랄 믹스 100 mgMineral mix 100 mg

스테아린산 마그네슘 2 mgMagnesium stearate 2 mg

통상의 사료 첨가제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.The above components are mixed and prepared according to a conventional feed additive manufacturing method.

제제예Formulation example 2. 소독제 조성물 2. Disinfectant composition

박테리오파지 ECOH7 1x107 PFU/mLBacteriophage ECOH7 1x10 7 PFU / mL

에탄올 적량Ethanol quantity

멸균 증류수 적량Sterilized distilled water suitable amount

pH 조절제 적량pH adjuster

통상의 소독제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.The above components are mixed and prepared according to a conventional disinfectant preparation method.

제제예Formulation example 3. 세척제 조성물 3. Cleaner composition

박테리오파지 ECOH7 1x107 PFU/mLBacteriophage ECOH7 1x10 7 PFU / mL

에탄올 적량Ethanol quantity

효소 복합물 적량Enzyme complex

멸균 증류수 적량Sterilized distilled water suitable amount

통상의 세척제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.The above components are mixed and prepared according to a conventional method for producing a detergent.

이상, 본 발명내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적인 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의해 정의된다고 할 것이다. Having described specific portions of the present invention in detail, those skilled in the art will appreciate that these specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

한국미생물보존센터Korea Microorganism Conservation Center KFCC11732PKFCC11732P 2017091320170913

<110> Optipharm.CO.,LTD <120> Novel shiga toxin producing Escherichia coli specific bacteriophage ECOH7 and antibacterial composition comprising the same <130> 1-66P <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 85260 <212> DNA <213> Unknown <220> <223> Bacterinphage ECOH7 <400> 1 ccccttaaca ttttcttaac aatttcttaa caatccctac atagttatgc ttttaaggca 60 cttgttagag acttgttagc cccttgttaa atctctgtta agaccacgta aaagccttgt 120 aagagtcttt agcatagcct ttaaagcctc tgtaaagctc tataaagagg tatctataca 180 gttggggtgt aattctagga agagacttaa aagagcttac aaggagctat agagagcttt 240 taagagggct atttagagaa tcactaagag ggaaagggaa cgataaggaa gagatagtga 300 ccatgttaat agcttgttaa aaagattaaa cacttgttag aaacttatta attaccttaa 360 caaaataact cttaaaaatt aacttgttag ctcttgttag tcatctgtta agattctgta 420 aagcactatc aagccttgtt aagaaactgt tacgttcttg ttagttatgt taagaatctg 480 ttaagacctt tggagagttg ttaagaagat gtgagaactg ttaagagatt gttaaggata 540 ttttaaaatt ttgcaaaggg ggtgttaagg agctgttaag ggctaccaag cagatataca 600 tcctagacag atatttttca gttccttcaa agaccctaaa aaatgacttg atagtcctat 660 tttataaaaa ttttaagatt ctgaataatt acctagacag tcttttaaaa atacaggcat 720 aaacaggctt ataagatacc ttgttagtac cgtgttagac cttgtgagat aaggtctgta 780 aagatattcc tgttaagttt gttaaaacag tctatataga ggcttagtag tgcctagtga 840 acacctatag agagtatcat agctgacata gagattaagg ggtgtataga ggcttctaag 900 agcgtataga gagatggtta aggtgattgt ataggttgtt aagatggctt gttagaagag 960 tgtttagaag gcttattaaa gggctattta gaagggttat acagaggggc tattaagacc 1020 actagatagt tactaagata ctatatagta ttatataact atatagagag taagattaag 1080 aaggttgtta agaaggatag atattttatt cacatatgat ggtgacccag aggttgggac 1140 aaccgataaa attttataaa aattaaaaag cccccaatta aggaggctct taagatattt 1200 taaatcagtg tttccagaac tgtttttagt gatgacttgg ttgtctgata aaaatgagta 1260 aagccattgt tgttatcaaa tacctgcatc atgatatcat ctcccttgat tgggtacact 1320 cggatatcat agtggaaacc attaaagaca caagagccat tacagaatgc actaaagtca 1380 tccactgaga tatactcttc atgtcctgtg atttttatac agataccttt actcggtctc 1440 ttaggattaa tatcttccat cacagacata ttctgtccac caagagatac catagtgacc 1500 actttacggt tcttctgttt aaagtacatc ttaactttct gcaaagtgtc ttcatagaac 1560 ggataagact tctctacaaa gattccttca agtggctctt taccattttc aacttctctg 1620 gtatgccgta ctgcaaagat aatttgttgg aaccagtcaa gattttcttc ggagagtgct 1680 tctcgaacat cactccagtc aagaactagg tagttgcctt caacttcttt taagttatct 1740 ttttccatat ctatcacacc attatcgtaa aacttggtaa gctggtactg caaacttgtt 1800 gctattgttc ctaataaggt cttcttctgt tactaagaat gtaatcccac ataagtgcgt 1860 atagtggctg tcatactcaa atattttaag taagtattta ccatcatgat aaacagattc 1920 tactacacct tttgcaacaa tctttccagt cttgaagtct ttaagagggt atgtattacc 1980 aactttaggg actgttttca cttcaggttc ttttagcttc tcactagctt ctacatcacc 2040 tagtaagtta gaactttcaa aagctctgaa tctcatataa gcttctgaac attcccagat 2100 aaaacaatgt acaagaacct tagcagcatg tcttgagcaa ttcaactcat cacagatttc 2160 tttgtacatt tcatctttag ttgaattggt aagcattgct tttcgaactg tttctttaat 2220 tttcataagc tctccgaata accctattaa gcatctctaa agtcagcttt ccagattcta 2280 aatgtggcac aataagtttt aaagcttttt caacatcatg cacaacatct attatacctt 2340 ctgcataggc ttctacaagg atatctttta cattttgtga tttacctttt tgtggtatca 2400 ctagacgcat atcaaactca tttgcttgag gatggttctt accttgccaa taaccatctt 2460 tgttaaagct gatgtagtca ccgtggtaaa aatgtacacc attagcttta aacttctcca 2520 gcacaaggca ttgaattggg tattcagtat tccactccct gactcctata accttcactg 2580 ttgcgatgta ctcctgtgtt tttgcatcta tgatgtcaag ttcttgacca gctatgattt 2640 gcatattcgc taccttctaa gttctacaaa ggggctatca agtaacccct caagttatta 2700 ctcagacttt tcagtcttct tgtcggattt cttactagtc ttcttctcag ctttctgctc 2760 aggctccttc tcagaagttg ccttgataac catatcaaga agctctgata agtcactctt 2820 cagtaaagta acaggctgct cgttgtagaa gtttttgtca agataaactt tgaactcttc 2880 tatagaagga aatccaaaaa ttggtacagg tgtcttaatc attacttagt ctctccatag 2940 gcatctttta agattgcctt gttaatcatt tcagcgattg caaaacgaac acatacagct 3000 ataattgtag gttgcattct ttacttacct cttcaagttt aacctcagaa tccttaagtc 3060 gtttcttatg aactctgaac cttattagtg agatgatgat aacagctaaa ggaacagcat 3120 acaagtacca aacagcatat acctttgcag taatctgtgt aatggtcaag tcttgagcta 3180 tacaactgat tgctgatact gccagtaaca ttgctaagaa gaacgccagt accataacaa 3240 tacttaggca tcctctagca tggtgttcat gcttaatgca cttattcttt tcgtcaacaa 3300 ccctgttgta gtcatttgta aagtctctgt agaaagcttt caagtattct tgagtgtagc 3360 cttcaaagca acctgtacca taccaataat caccaccaaa atgtgctatg ataccagtat 3420 ctccatttgg tttagttact ctcaaagatg tgtgatgacg agtatcttga tagtctattt 3480 tgtatctttc ttcgtaaatc atttcccaac ccctctcaat cgcttgttcc attcaaggtc 3540 aataaacctt tgcaggactt tccctctagg tgttacacct tttaaatctg catcgtaaca 3600 atggtaaaag tcaccttcag cgtgtttacc atagcatttt ctgatattct ggcttctgcg 3660 atactgttct aacaaggaca gtttgtagta atcttcaact gtagttagtt ttttcattct 3720 ttcaccgtag tgtttctcca gaaactaaaa agggaactac ctctcgatag ctcccatcat 3780 aatcaatatt gtatactcgg tcaagagtat tttttaggca gtaatgcgct acgcttgtta 3840 ctctcttcac tcaatagtgc gataaattca tcacctttcc ctttccacgg actgaatgta 3900 gggatgtgct ctctgatagc ctcaatcact gttttcaggg cattgttctt taaccaccaa 3960 tcagacactg ccgaagctac gatgtagtaa gcaccgtcat ctgtgaagat tggctcagag 4020 atacatggtg aaatcatagt ggtgatgata gagccattat tcatgtaata tgttcttttt 4080 tcaaccttat caagaagctt gtgaaggtct gcaaagagac ttaccacctc tttatggtca 4140 atatcttttt caagatttgc tctatacttt aatagtgcag catcaacctc tttatcatta 4200 aatttgaagt actggacttc ttcattcaca catacactga tgttgttggt gtttatcgtc 4260 aatttgcaca gtttgactgg agagttaatg ttggtctgaa aaagtgctac acagccttct 4320 acatttccga aatgcttgac taatgtaact tctggttgga tatgctcatg ttcttctgac 4380 attacagttt cctcattaaa tactttttaa gttgtgtgtt aggtttctca aaaacttcta 4440 tagaagatat cttaacagac tcaccacgca taccagtatg tttgaatatg tagtactcta 4500 acatccctat ggactttgct ggtaagtcat gcttgacata caccgttaca gaatcttcaa 4560 attcaacaac ttttgtacca tctttgtagt tgaactcttt aggatggttg taattgtcta 4620 tgtcatacat taaaaagagt cttttccgtt gcatatcaag cttgtttgtt aaagactctt 4680 tgatatctga gaagtttttg taaactgtga catggagctt actcatcgtt aaactctctg 4740 acgaagaaat agttgacttt gttattacct accataaggt caaagcaggt attttcaaaa 4800 ggtttgttgt aggcatcgta tgaaatatat gggtcagcta cttgcttagt gcttaacttc 4860 ttacttgatg ggtttaagag gccaccatac agcttcttga tagcctcttc tacaacagac 4920 cagataattt cgtcagtaat ttcttcgtac aggtcaagtt cacgctctag tgagaagtca 4980 caaataggga aaataacctg tactttactc attaacgaat aacctgtgtg acttgaattg 5040 cttcagttac tgataagtca cctactttct cttctttgat aaaggtgaag gtgagttcaa 5100 caggttcttg ctcttcttgc tcaactggaa tgccattaag ctcatcaaag attgattgag 5160 ttgtccagtc ttctccacca tctggatagt ggattgcata aagaggaaat tccacgtcat 5220 accagccaaa tacagggcaa ccgtctacat catcacgtac tcgtcgaaga gtgttgcctg 5280 attctgtaaa tcgaaggctg agattaccat tacactcaaa aattactgca tcagcaggcc 5340 caacattttc tcgaccatca tgaccaaatg tgtcaacctt tacttgaatc tcgttattca 5400 taaaaacctc tctctgcttg ttcgattttt gctcttaaat tttcgcaatg ctgtagatgg 5460 tagttagctt cttgttcatg tgttgctaac tgcctttcca gaacagtttt atagtaacct 5520 gcaagttgct cttttgtcaa tgggtgctct gaaataatat caagtccact ttcgccaaga 5580 gggttgaatt gtccatgctc attgacatgg taagtttcaa acttgaacag accgttgtta 5640 gcagattcaa taggtgagag ttcagccaac tcaacagtga acattgtgtg taccctatca 5700 ccaactgtaa caatgttact accaatgatt ctcaccattg agccattgcg agtgtactta 5760 aaagttccaa agtcactagc ccaaagtttc atagttttct ccttagaaaa taaaaaggct 5820 cccgaaggag cctatgaaga tattactttg ggtatacact gtcaagataa atgtcagctt 5880 ccattctacg tctgtttttc aggccgtttg aagtgacctt cttacctttg actgtaacct 5940 tgttccacca ttgcatagct tctgcacaac ctaccttatt accatcattg tggcgcttga 6000 taaatgtaga atcctgcata gctgtgatac cgatgttgta tgtttcactt acaagtgcat 6060 cgaactcgtt ctgagtagtt ggagccttga tagctttatt cactgctgca acgaatttct 6120 caacatctgc gagaagatac tgttcagctt gttcagccgt gattttcata cccatcttaa 6180 caggttttcc gtcaatacgg attgtaccat acccgattgt tgggattccg gcagaatctt 6240 tgtaagcctc taacttcaga ccttcaaaga acttaatggc ttctaagcct tttcttgaga 6300 gttgcattat gcctcccctg ctgggttaac tgtaactgtt gctgcattag atgtcacaga 6360 gccacctgca ccagtaacta cacaggtgta gcttcctgaa tcagcagttg ttgcaccttc 6420 tttggtgtaa gttgcagtag ttgcatcgag gatgtcttcc tcacctttct tccattggta 6480 acctgtcgca cctgttgcca caacattcag tgttaaggtc tcaccttcag tgatttcttg 6540 attggctggt tgctgagtaa ttgctggtgg cttaatagca tctttcaact tagcattcag 6600 cattgagaac ggcttaactc ttgccagcca ttcacagtaa attgtgtcaa catctttacc 6660 attgataatt gcatattgta agtccatgaa gaagtcagaa gttctcattt gagcaccaat 6720 gctgtaaagc agctcatcac taaatggaac tttatagtct ggtttgtagt caaacttctt 6780 aacttctgca atgtcagctt caggccaata cgaactatag gtgagtggta aaatagactc 6840 ttgatatgtt ctaaaatcat atctcttacc agtctttaca tttgcaatga agcccttcac 6900 aaactctttg aagtctgggt aagtctttgc tttaatcatt tctttttagc ctctaaatgt 6960 gcgtttagtc taaaataacc tttgtggtgt ggagatacat caccaacaac aacaccttta 7020 accttataac ctttaccaat tacatactgg atatccataa ggaatctacc aagaccaaca 7080 atatggaaca ttggatataa tagattctca gagtctggaa tattgatttc aggtttgtag 7140 gtgcgttgtt cgattgttgg tgtaatatct tcttcaggcc atttactgag tctccatgtc 7200 aaaggtgttg ggataacatc attaaggttg tatctttcac cattttgcac ggaattgata 7260 taggattgta ggaagcaata aaagtctttt gcactcttag ctttcattaa atatccttat 7320 aaatagttaa aggggcatca gcccctattc acacttaagc tttggtctta agtaggttct 7380 taagtttttt aagattcata ctctcatcaa acttaaaacc aaatacattg acataatcaa 7440 tgaactcgga tttcttactg agtgacaatg cgtaattcat atcaaagttc ttacttgttt 7500 ctgcgtctgg actagagttc acatcaaatg agatttctgt aggctcttgc ttactttttc 7560 tcatattatc agtcaacaaa catgtatagt ttgctggata gaagtttaca ctaccatcaa 7620 cgtagctgta cttattatta tggataaggt tggaaaatac cttaaggaat gtttctgagt 7680 cgtggcaaac aagtttgaat tggctgctct ctccaagtgc aactaatgag tacggacttt 7740 cactatcgaa gtcatagttt ggattcgatg tcgtcactgt tacacgcatc aagttattag 7800 caatagtctt ctgtttaaaa gaatttggaa catagtgaag acctctacca cactgattga 7860 ctagctcttt aagtaggtca tttgcatacg gtgaattaat tgatacagag ccatcactat 7920 tgtaattaaa acttactgtt aacatttttc agttctcctg cgatttataa ttttgctgca 7980 atagagaaac gaagtaaact cactggaaga aaccattaag tgttgttcac tagattgagc 8040 cattgccctg aaatacattc cagtcaaagc atcatagtga ctattctttc caattgaggt 8100 tatatagcca tgactctttg cagtttgata taaatctata atatctgcga tttctaattg 8160 ggaactacac atagataatc ctcttgtctt ctgtaatttt gaaagaacag gtacagacca 8220 atttcagagg atgaatagta gattaactct accctttttg attccttaca gtgattggta 8280 ttcttacctt tcttaggttt atttgatttt ctcattaagt ctcctttgta gaacaaggca 8340 gacctacagc tttaagtaag atggtacagt aatccataca acaagtcaag aactattgca 8400 agttaaaaaa gttgttgaca aggtacttga caaggtgtag gcttataaat acttagaaga 8460 taacttaaaa ggttcctcta aaaaggctat ttaaacagat atctttctgg atatctatct 8520 tttatcttaa aagcctttta agaggtctta taagaggcat accaatgaaa aacagaacaa 8580 acaaaggcca gtttaaaaaa ggccagtctg gaaacccatc tgggagacca aaaggctctc 8640 gtaacaaaag ctcactcgta aaagctcaac tgaccattga taattctgct gagtttgctg 8700 caaagctgtt tgaggcgatt gttacaaggg acgctgctaa gcttgcagag ttcggtttga 8760 cgactgccga tgtaaacctg aagtcgatga tggaagctgg taaaactatc atgacccact 8820 cagcaggtga gatgaaagcg attgcagcag acactaagaa gacccctgat aatggtggtc 8880 agtcacagac agataacaaa ccaacgttct ctgctgtagc gactctcaaa aaataatttt 8940 aaaaggtgtt gacagactct acagagttgc tctaaagtct gtcacatcaa acaacaaatg 9000 agagaagaga gtaaaatatg agcgaattat ttaaacatgc gcaccttcat gcaggtcgaa 9060 ctgaaaatgg tgctgtaaac catacttcat caatgtctgc tcttgtggac ttttacaaag 9120 ctgctggttc cagccgtagc aatgtagaaa tcttaccaga cctgttctac aaagctttgc 9180 gtgaggatgt tgatgttgca gttcgtattt tactgcatat gcgagatgta cgagaaggta 9240 tgggtgagcg taaagctttc cgaactgttt tacttcaagc gattgaagat aaagttttag 9300 agcctacaca ggttcttcgc attatggata agattgcaga acttggtcgt tttgatgact 9360 tcaaaatctt cgtaggtact cgttttgaga cagatgcctt caaacattta gaagcagcat 9420 tactagaccc tgcaacagca ggtttagcgg ctaagtggtt accacgagta aaaccacgcc 9480 acaaacagtt tgtaaaacgt ttctgtaagt ttgcaaactt aagtgagaag gagtatcgca 9540 cactgttatc tgcactgtct gatacggttg agcaaaaaat ctctgctaat gagtttggta 9600 agattgacta cagcaagatt ccttcactcg ctgctgcacg ttaccaaaag ctctttaacc 9660 gtaaagatgg ggaacgttac aaagcttaca tcgagtccct ctcaaaaggt gaggttaaga 9720 ttaacgctgg tgctgtttac ccatacgatg tgattaaatc tatcaagtat ggtaatgcag 9780 atgttgctaa tgagcagtgg aaagcactac caaactggat ggcagaaggt gagaacatct 9840 tgtgtatgac tgatgtttca agctcaatgt cttgggtgaa tcttggttca atcactgccc 9900 ttgatattgg tgtatcactt gctttgtatg tagcagaacg caacactggt tgctttaaga 9960 atgagttaat ggtttactca acaaaccctc acttcatcga actgagtggt gatttacgaa 10020 accgtcatcg tcaggtaatg cgtcatgttg aatatggttc aactaactta caagcagctt 10080 ttgaccgtat tcttgaggta ggtaagcgaa acaacttgac tcagaaagat atgccaagta 10140 agcttattat cttctctgat atggagttca ctcaggttga tggagctaac ggtcgtacaa 10200 actttgaagc aattcagagt aagtacaaaa aagctggata cgaaatgcca caactagtat 10260 tctggtactt agcaaaccgt aatggtactt gcgaagtatc tgttaaggat aatggtgtag 10320 cgatggtatc agggttctct ccagccactt taaaagctct gcttggtggt gagaagtttg 10380 acccaatcag cgtaatgctc aaagcagtaa tgattgaccg ttacatctgg taaaaagttt 10440 taaaaagggt attgacaatg tgtttgatac cctttaatat gttctacata gaaacgaaat 10500 gagagctttt ctaagatact gaaaaatatt ttaaaaaagt tcttgacaac cactaaaaaa 10560 taatgttaaa gtggttacat agagtttgaa aagtttatct ctgtttagct cagcttggta 10620 gagcgttccg tttggggcgg taaggccgga ggttcaagtc ctccaacaga gaccaaatta 10680 atgttccagt agacaaaatg gtaaagtcac cactctttca aagtggatat ttgagggttc 10740 aaatcccttc tggaacgcca gttttgacag aagaccaatt gcagcaaact taatcttatt 10800 catctgacac gaaatcggac aaagaagaga tttggtctgg tcattaagaa ttgcgggtat 10860 agagaaaggg cgtctcacat gtctcattag catggtatcg gcaggttcga ctcctgcacc 10920 cgcctccaaa tttacagaag accgtttaca gcaatattta ttaaattttt agcggaaaaa 10980 ttgaaagata cggtctggta atactaaaca tggttaggaa gcacataagg tatgtgcggt 11040 cgcctgttaa gcgaatggca cagggttcga atccctgact aaccgccaaa tttaaatgtg 11100 tcgttatccc gtagatggta gcggtgggga ctgtaaatcc cttgtcattg agactcggta 11160 ggttcgactc ctacacggca caccaaataa aggttatgta gtttaatctg gttaaaatac 11220 tcccctgtca cgggagatga tgagggttca aatcccttcg taaccgccaa attataataa 11280 gtatttcaaa atgatgtttg agagggctat ttagcccctc ccttaaagag ttcttacgag 11340 tattctttaa aggagcagaa ggccaaattc agcaagttac ttaaaatttc aagccaattt 11400 aattttgaaa aatttaaaac ttggtctggc tcaacaattt tacagaagac cgtttacagc 11460 aaaacttaaa caatctattt ctccggtaaa gaaaaggcga aggttcgatt ccttcactcg 11520 gcagatgtcg agttggtgta atggtagcac ttaagatgat aaacaacggt ctggtaatta 11580 tctttgtagg tgagctatta agcttacctg tgagtatgcg accacaaagg gtgaaagggt 11640 aaattctaga tttaaaccca acaaggcaca tcgctacaga ccttaagcaa gtctgggtag 11700 cttatctaca aagataattt aatggaagtg tagcagaatg gtgatgcggc agacttttaa 11760 tctgacaggc gatgggttcg aatccctcca cttctaccaa tatggttcag tcgcagataa 11820 ggtaatgcag gggtctcata agccctatga atgtgggttc gattcccatc tgaacctcca 11880 agctggtata gttaagaagg ttataacact cccctgataa gggaacatcg gtggttcgat 11940 tccacctacc agtaccaatt tcaataaagt tgttgacatt gagatgtgac tactttatag 12000 taactgaaaa tgattcagca tatgtataat tcaaacacca atacatgagc attagacgta 12060 atgcaatgtg gggaaatttg ttgtacatat tctgaatagt tttcgttgcg tagcgtctat 12120 tttgcaaatt taaaataaat gcaaacgaca atgtttttct ggcagtagct tgataggcta 12180 aacaccagtg aggtcttcca atccctcatc aaagaatttg gcgtactagc ccacggtatg 12240 attaataagg tgggcaacat tttaaagggt atcccttatt gggcttggct taaatgcaat 12300 gagtaccctt taaaatgtta attacggggc gtatctcagc ggtcttctaa accgccgtta 12360 accgagtaag tggagtatgc gggttcgagt cctgccgcct cgaccaattt agttagcaga 12420 ttgcttagac gaccttatcg ggtctccgtg taaggaggtg gtctgtatct catgttttcc 12480 agaacatgta aataatctga gaaggggctt tacagtgttg tgaaaatacg gtaagcttcg 12540 aattacaata tgggacgatg ttaagactct aaggcatgag caacggcttc caacaccgtt 12600 tcaaaggggt tcaactcctc cgtcccatgc caaagtctta ttaggggtag gtagcggcta 12660 atggtagcca aactgtcttg aaaacagttg ccactgtaga gatacggtga gggttcgact 12720 cctttactta ccgcctaata agatttacac cgtccatgct tgctcgcatt ttcggtttat 12780 ccatcgtgta ttgtcgtaca cactgataaa gcacctagta ggtgtcacgg agaagagata 12840 atatcaagct ctccaaaggt tctagtcacc ggattaaaca agactatgca aagacctatt 12900 taggtctttt tagagggctt ctaaggttat tacagcttgt tgtggaacct tgttgcttag 12960 gttcttagaa gttctctaaa aagatttaat gggagattga cggtaattgg taaacctacc 13020 atccttagaa gttggtgttt gagggttcga atcccttgtc tcctaccaaa ttaatgcagg 13080 tgtagcaaaa tggttatgcg gctgactctt aatcagtaag acgatgggtt caattccctc 13140 cacctgtacc aaacattggg gatgtagttt acatggttaa aacataagtt ttgcaaactt 13200 aagtacaggg ttcaattccc tgcttctcca ccaattagtg catccatagt ttaaacggga 13260 aaattacagt cttccaaact gaggttgagg gttcgattcc ctctggatgc tccaaattaa 13320 tgctcctatc gtataattgg ctattacggt tgccttgtaa gcaacttatc agggttcgaa 13380 tccttgtggg agcaccaatt cagaggtcaa gtgaaagacc gctggtgtca actgaagacc 13440 gtaacaaatt ccacggagtt gagttagcgg cacaacttca gacctcttcc atactcgctt 13500 agtttatatg gtaaaacatc acccttacaa gatgaagaaa aaggttcaag tcctttagtg 13560 agtaccatgt tccagtatcc caattggcag aggatgcaag ctcaaacctt gtactagtga 13620 cggttcgaat ccgtcttgga acaccaattt tgcaggatta gttcaaatgg atagagcaac 13680 agtctacgaa gctgttaata ggggttcgaa tcccttatcc tgcgccaagt taaagaggct 13740 atttagatga gatatttgaa gtatgcttca tggattttct tagctttgtt agaaccattt 13800 gcagcaatct tagcagttat cttagcacct tttgtagttc cattctacag tgagaagaaa 13860 ggacatctac ctttcgggtt cagatggatg gaaacttatg acaacccaat tgatggcgat 13920 gaaggtcatg tcaaaagatg ggctaagatt agaaagattg gtaagcttgg tatctatatg 13980 cagagagtcg gttggctctg gagaaacaaa gcttataact tctcttacca tgtgttagga 14040 agagatgtaa aagatgttac taagtggaaa ggtaatatca atgtaagttc taaccctgaa 14100 gataatcaga caggttatct cctaatgtgg aacagtaatg cttgggggtt attcgctttt 14160 gtaccatcaa ttaaaatctt tggtaaacaa ttctactgga gaatttatgt tggatggaaa 14220 cttaaaagtg ttgtcccaga agaaagagca ttctcaaggg aaagagttat gttggcattc 14280 tttatccatc cactaagaaa ataaagattt aaaggggatt agtttacaag gttaaaacct 14340 cggtctttga aatcgaagaa gttggttcaa ttccaacatc ccccgccaat gctccattac 14400 tccaattggc agagaggcca gacttaaaat ctgtgttatg tatcggttcg aatccgatat 14460 ggagtaccaa atttagcggt atagcataac tggcaatgca gcagtctctg aagctgtctt 14520 attaaggttc aaatccttat gccgctgcca cttctaagga ttcttacgag agtccttaaa 14580 tgtggcctta tcataaatgg taatgaccca tgctgtgaac atggtctata cgggttcaaa 14640 tcccgtaggt caccccaatt tatagtccaa gtagcttata tggttaaagc gcgtgtctga 14700 aaaacatgag aagagggttc aaatcccact gggctaccaa tttcaaaggt gcttaatgaa 14760 agagatgaca gaacaaggta aggagatttt taatctctta aaaaccggta gagggttttc 14820 taatcccctt attactggtg cagcagttct cggtggaacc gtagctgcct ctacatcact 14880 tgtaagctct attagctctg taacagaccc tacaataaag gataaacttg ttgctgctgg 14940 acttacaaca gttctgctta acagctttac aacgagtctg acaagcacta catcaaccac 15000 taagacattg gcagattacg gtcaaaagtc cattgatgag ttttcatcac gtatgcaggt 15060 agcaaaagga tattccaatg ttatgggtgc agcaggagag caagtcggtt gcacaccatt 15120 tagtggtatt atgggtgttg ctacagagta cggtcaaaaa gctattgaca caattaacag 15180 tacactagat ggtgttaatg atgtattaag tgacttacaa gatgctattg ataaaggtct 15240 tgatactgtt tctgatttag ctaatcaagc tgttagtaag attaatgaag gtatctcgaa 15300 gattacagct tatgcagatg aagttgtgca gatgattgaa gaagaagctg cccttattgc 15360 agagtacctt aaaacgaata tcaatgggtt cttagcaggt atcttaccag actggtttga 15420 tgatgcttgt aaaactggtg taattgacac tattgcaaca ccagaaatga agaacgcatt 15480 acagaaataa tggaagatta accctaaaag gtaagggagc agtttgctaa actgccagta 15540 gctgagaaat cggtgtacca gttcaagtct ggtatcttcc tccaatttga atccgtgaca 15600 gaaatggcta tgtacctgtc tgcaaaacag gtttataagg gttcgagtcc cttcggattc 15660 tccaagttat tttactctct ctcaattaaa ataataataa tgccctgcta taagtattct 15720 tcctttcgct accgaagagt atttttagac agggcttttt tacagagtta tactttataa 15780 gaagacttat acagtatgtc tctgacgtac attgtggttt ctccttgacg gtctctgtac 15840 cgtctttttt aaggagaaac atggttaact atttggagaa aacataatga gcgagaccgt 15900 ttataaagaa ctttatgaag ctaataagaa gctagaattt atgcagaata ctatcatggc 15960 aattgcagac agattatctg tagcaacagg tattgatatt aaagaagcgt ctatggatgc 16020 acttcttgac gctgttgatg caaagttcga agttaagaaa gaagagactg ctacagactc 16080 tgaataattc tatttgcgga ggtgcttaat ggacttaaat gctgttaagc agaagcgagt 16140 ggaagatgtt aggaaagtcc tagctggaga gttggggctt tctgatgaag taaaagaaat 16200 tatcaaatca ttcggtaaag acccctctaa attccttcca actcaaattc tgactttatt 16260 aagatacaca ccagaccaag ttagacttat cttcaaattg atgactgata agaactatgt 16320 agcccctcag ccgggttctc aagaggtctt tttaaatact aatgctgact tggttctcta 16380 tggtggtgct gcgggtgctg gtaagactgc tgcattgttg atggattctt taagatttat 16440 tgaagaccct aactataatg ctgtgtattt ccgtcgaaac acgacacagt tacaaggtgg 16500 tttatggcct gctgcaaaga aactatttgg taagtttggt ggggttcctc acgagcagaa 16560 gatgactatc acattccctt ctggggccac tatcaagttt acctacctag aacttgaaaa 16620 gcacgctgaa ggtcatcagg gtattgaata ctcagctatt tactttgacg aaggtacgca 16680 cttctctgct tcacagattt catacctaca gactcgtcta cgttctgggg ctgaaggtga 16740 ttcatacatg aagatttcta tgaacccaga cagagaccat tttatttacg attgggtaga 16800 gccattctta gatgaagaag gttacccaga ccctgaaaag tgtggtcgta ttcgttggta 16860 tgtaatgaat gatggtgtta tggtttctga ttgggagaga gacaagattc ttgaaatgtt 16920 tcctcttgag attcctcaaa catatacatt catctctggt acgattgatg ataacccaat 16980 tcttgacttc ttagaaccta aatatcgtgg taagttggaa aacaacaccc ctgtaaacgt 17040 tgcaagactt cgtttcggta actggaaggc tcgtgcagaa ggttcaaact attggcaaag 17100 acaatggtgt gaaattgttg attcacttcc agaagatgta ttcgatgtca gagcatggga 17160 cttagcagca actttaccat ctgagattaa ccctaatcca gactggacag caggtgttaa 17220 gatgggtaaa tctaaaaaag acggttgcta ttatatcatt gatgtagtaa gatttagaga 17280 tagaccctct ggagtcgaaa cacaaattaa tttgactgct gaaagtgacg gtaagcgaac 17340 tggtattttt atccctcaag acccaggcgc tgctggtaaa tcctacgcaa catccctcat 17400 caggaaactt gccgagaaag gttatcgagc aagggctaaa ccaacaaata aagataaagt 17460 tacccgattt gcggggtttt ctgctgcttc tgaagctgga cttgtaaaag tcttgagagg 17520 tagttggaac gaagcttact tccaagaact tgaaggtttt tgtggtgatg gtaaaactaa 17580 agatgaccaa gtggatgcta ccagtgatgc tttcaacagt cttaacgaag ttaaattatt 17640 caagccacca tcaatgggtg ctcacacaga cttagtgaga ggaaacccat atgaggggct 17700 tagacgttga tagctaggtg agaagaatgg cagatattac agaaacacaa gaaagcttac 17760 caccatttag aatgggtgaa gtaggttctt tgggtctgaa ggttaagaat ggaagaatct 17820 atgaagaacc tcgtcaggca ctaaggttcc ctgaaagtat taaaactttc caattaatga 17880 tgcgtgaccc tgctgtagca gcatctgtaa atattattaa gatgtttgtc agaaaagtca 17940 actggagatt cgtacctcca aagggaaaag agcaagaccc taaaatgctt gaaagagcag 18000 acttctttaa ttctttaatg gatgacatgg agcatgattg ggcagatttt attaactctg 18060 taatgtcatt ctgcacttat gggttctgtg ttaacgaaaa ggtttataag aaacgtcagg 18120 gtaaaaaagg aaagtatcag tcaaaatttg atgatggtct aattgggtgg gctaaattac 18180 caatcagaaa ccagtcaaca cttgataagt ggtattttga tgaagacttt agaaaagtta 18240 ctggtgtcag acaaaatcta agaaatgttt cacatattgc tggagcaatt aatcttggag 18300 aaagaccact aacaagaaaa cttccacgag ctaaattcat gctgtttaag tatgatgatg 18360 agtatggtaa cccagaaggt cgttcaccat tacttaatgc ttatgtaccg tggaagtata 18420 aagtacagat tgaagagtac gaagctgttg gtgtttcaag agacttggta ggtatgccaa 18480 agattggttt accaccagat tatcttgatg aaaatgcaga gcctgaaaag aaagctttcg 18540 tacaatactg caaaactgtt gttaatgata tgattgctaa tgacagagca ggtttaatct 18600 ggcctagata tatcgaccca gatactaaag aggatatttt tgagttctca ttagtttcta 18660 gacagggtgc taaagcgtat gacacaggtt ctatcattga cagatattct aagcagatta 18720 tgatggcatt tatgtcagat gttcttgcta tgggtcagtc aaaatatggt tcattctctc 18780 ttgcagattc taagacaagt ctattagcga tgtcagtaga tattctgctg aagcaaatta 18840 agaacgtaat taaccgtgat ttagttgcac agacttatgc tcttaatatg tgggatgacg 18900 aagaacatgt acaaatcaca tatgatgata tcgaaactcc agaccttgaa gcaattggtt 18960 cttatattca gaagactgtt gcagtaggtg ctttggaagt tgacaaagag ctatctaaca 19020 aacttagaga gcatattggt ctccctcctg ctgatgagtc tcagccagta tctgaaaagc 19080 tttctccaaa tagccaaagc cgctcaggag atggttataa gaccgctgga gaaggtactg 19140 caaagacacc ttcagcgaaa gacccaagca cagcaaataa ggcaaataaa taatggctga 19200 aattatctct atttcaaatg ctacacgagt gcattcgtat aggggtgtcc ttatcattac 19260 tgataagcta tctgtagagg ctggctcaag ggtcagcctt tcaggttatg ttagtgatgg 19320 tggagtctct gacgttttca ctatttgcag gttacttgat gcaccaatga gtggaaaacc 19380 gtttatttca ggaaattgta gtgaaattgt taaaattcca tttgacagtt catgcctttt 19440 gggtgtaaag ttatataact gcgagaataa acgtatcaat gttaatagca ttgaagccgc 19500 tttcattacc ctcgacactg catttcaatc cccaatgaca gttaacaaag aaacaaacag 19560 acttgaatac attttttcac aaaatgatta caaagtactt gtcaaaggta aagtatatga 19620 tatgattgta aatgtggtag atgaatctgg taaccattca acagtcctta aacaaaaagt 19680 aaggtttaat taatgggaac attaactatt gatggtaaga ataaaatcct cgccacgcta 19740 accccaacga ctattatttt acacaatgta gacccaacgg cagaccctac agcaaataag 19800 gttactcagc cagtggctat ttatttttct gaaccggata atggcttaat tgcctcagaa 19860 gacacagtta atattactgt tccagcttct gcaacggtct cacactatag cttgtgggat 19920 gctaacgata aatgcgtggc aactggtgct cttagcaaac ctcaattctt tgctgaagaa 19980 ggtatctatg ttatctcttc agtctctgta gatttaaaca aatagggtga gtaaaaatga 20040 gttcgaatat cttcagactt gctgatagat tattcaacca acctttacta gccactgaat 20100 cattagctca ctcagcagca acttatgtga ataacagatt gctgggtgat gtccaagcag 20160 cagtaaactt tgataaaccc aaaggtgaag caagaagtct tttaaaagta aaagatgaca 20220 ttgctattat tcctattatg ggtggtttaa cccatcgtat gacattcatt gatgcaatgt 20280 gtacaggtgg attaagctct tatgaaggtt tgcgtagagg ctttgacgaa gctttagcag 20340 atgagtcaat taagactatc gttctgcata ttgattctgg tggtggtgaa gcttcaggtt 20400 gctttgaatt agcacgtcac attatggctt caagaggcca aaagaaaatt attgcttatg 20460 tagatgagtt cgcttgttcc gctgcatatg ctcttgcatc ttctgctgaa gaaattattg 20520 catcaccaga tgcagatgtt ggttctattg gtgtaatcat ggttcatcag gaattaacta 20580 aggcatttga aaagaatggc gtaacaatta acgtcatcaa agctggtgag tttaaaggta 20640 tgggttcacc attccaagca ctctcagaag aaagcaaaga aagacttcaa aagagaatta 20700 atgataccta cgcaaccttt acaggttttg tagctgaatc tcgtaatctc tctgaagaag 20760 ctgtaaagaa tactgaggcg aatgtttatt ctgctcagga agctcttgaa cttggtttaa 20820 ttaactcaat catgtctcaa gatgatttct taaattactt acaaggttct gaagaggctc 20880 ctgtaagttt aaacgttaac aattcaggtg aagaaatgac tgaacaagaa aagcaagaac 20940 tagaagcttt gcgtcttcag gttgctcaaa tgaaagctaa agaacaggaa gctgctttgt 21000 cagatttgac taataagatt tctgcttctg ctgaagcttt tggatttgat gcaaaagaag 21060 ctgcaacgac tattttaggt gctggtcttg ataaccctct gagtgttctg tttatgaatg 21120 ctatggaagg tgctaaccag aaacttaatg aaactatcgc atcccatgct tctgcaatgg 21180 aagaaaaaga atcagaaatt accaagctga aagaaactgc tggtgctgtt cttgaacatt 21240 ccaacgctat ggaagaagtg ggtaatgacg gcgaagctga tttggttgaa gaagaaaaag 21300 aaccagctaa gaatgcttcc gaagacaccg ctgaacaacg caaactggct ctccagaatg 21360 ctctaaaatc tcttatcaaa taaggaacac aacaatggca tatcaaggtt ttactaagtt 21420 aggtaaaaga gaacctctga atgatatcat tctttgggaa caggttaccc caacaggcca 21480 ctctcgtaaa gagtacaccc cacaggcatc tacagaatat cgtgtaggtg aagttctgaa 21540 agcagacggt actaaggtta cagcagggca agaagctcag gctgactctg tatgtatcgt 21600 taacttctac gcagactcac aactgtctta tcatggtcag ttgaaagttg ttggtattta 21660 ccgcgacgca gaactgaaag acatgcttac tcttgaagca agcgttgacg ctgccgaagt 21720 caagaaagct ctggctgcca aaggtattga tttcgtacca actggcctgt aataaaaata 21780 ataagacatt ctggagaatt ttacaatgtt gactaattct gaaaaaagca gatttttcct 21840 tgctgacctg actggtgaag tccagtctat cccaaatact tatgggtata tttccaactt 21900 aggtctgttc cgttcagcac caatcaccca aactactttc ctgatggatt tgactgattg 21960 ggatgttagc ttgcttgatg cggtagaccg tgatagccgt aaagcagaga ctagcgcacc 22020 tgagcgtgtt cgtcaaatca gcttcccaat gatgtacttc aaagaagttg aaagcatcac 22080 tcctgatgaa attcagggtg tacgtcagcc aggcactgca aacgaactga ctactgaagc 22140 tgtagtacgt gctaagaagc tgatgaagat tcgtaccaag ttcgatatta ctcgtgagtt 22200 cctgtttatg caagctctga agggtaaagt tgtcgatgct cgtggtactc tgtacgctga 22260 cctgtacaag cagttcgacg ttgagaagaa gactgtttac ttcgaccttg ccaaccctaa 22320 tgctgacatc gacgcttcta tcgaagaact gcgtatgcac atggaagatg aagctaagac 22380 tggcactgta atcaacggtg aagaaattca cgtagtcgtt gaccgtgtat tcttcagcaa 22440 actggttaag caccctaaga ttcgtgacgc ttatcttgca cagcagactc cgctggcttg 22500 gcaacagatt actggttctc tgagaactgg tggtactgac ggcgttcagg ctcatatgaa 22560 caccttctac tacggtggtg ttaagtttgt ccagtacaac ggtaagttca aagacaagcg 22620 tggtaaggtt cacactctgg ttggcattga tggtgtagca gcaactgttg gtgttggtca 22680 tgccttccct aacgtatcta tgctgggtga agctaacaac atcttcgaag tggcatatgg 22740 cccatgtcct aagatgggtt atgcaaatac acttggtcag gaactgtacg tatttgaata 22800 cgaaaaagac cgtgacgaag gtattgactt cgaagctcac tcttacatgc tgccatactg 22860 cactcgtcct cagttgctgg tagatgttcg cgctgacgct caaggtggct aataaactta 22920 ggagggttat gaatgtgtta tacaggcgac ccagccaata accctcttga tagagtaaga 22980 atcctctgca cagacactaa taatgatgaa attcttattg agcagtctgt gctagagtgg 23040 ttctatcaag aatctggaaa ggatgaaaag aaagcagcca tcaaagctct taaatattta 23100 ctctttcaag tagccaagat gggagatgag aaggttggtg gtgtttactt acgtaactct 23160 tccagattca aatctctgaa agctgtttat gacgaccttg ttaaaagctc tgtttcagga 23220 ctaccctatg caggtggtat taaccagtgc gacattgata tgcgtcgtca gaatccttgc 23280 tctgtcaaga aatacacaga atatggtgat gctgccagat acgaaggcag agattactgc 23340 aaccgtgtta atggcgtatt tattatcgag cgagatgaat aatggttaaa agggttattc 23400 accccgctag agcaaaatta gtcggggcta tgaagaactt gcaaacggct aatgctcaag 23460 ttgggtattt tcaagaacaa ggtcaacata gctctggttt ttcttatcct gctttaatgt 23520 atttacaaga agttattggg gttccttcag cttctggtaa agtatatcgt aggttgtttg 23580 aaatcactat gatgctaaac aaacagacct tgttagagca gactaagaag aatctatata 23640 agcaacttag cagtctcaac acagaccctt caaatacctt agaagcattt gcaaagaatg 23700 ctcaaaaggc aattaaaaga ggttttggta attctgctat cctccctcct aacgcacctt 23760 ccacagtcaa gaaaaaaggc tttaatgcac ctcttgttga gacaggtgac ttaagagata 23820 gccttgctta taaaatttct actaagaagg gtattaagaa atgagactct taaacagaca 23880 cagctttata gtaaagcgta aagtctctga agacggttat tataatgatg atggtgattg 23940 ggtagcttca caagatattg tagaggttaa ctgcaaaggt aatatccagc catatatcaa 24000 aggttctgta aagaatggta cacagattgc tttaccggaa ggtattaggc ttaccgatac 24060 aagaatcctg tataccacat ataaacttag aacttcagat gatgtagagt ggaatgagtc 24120 tgacattgtt atgattgatg gtcatgagta tgaagtattt atgactatgg attggtcaca 24180 gcaattagcc catacgtccc attatgaata tatcattatt agaagggata aaatgaatgc 24240 agttagaaac agcagaactt gaaaaaggtc tagttagaac cttagtggat gttattggtc 24300 acagactaac tcgtgataaa aataatagac caaatgtaat tagagcttac ccttctgata 24360 actcaaatga caaaggttta aaacctgacc agccatttat taccgtatat tgtcaagatg 24420 ctgcaacacc ttatggttgg gttcttgata agtttgttga ggatgatgta gtttgctaca 24480 gaattgcttt tcagattcct gtactaatta cggtgaatgg taaaggtgct cacagtatta 24540 tgcttgagct taaacaacga ttagagatga gttcagtcag ggatttaatt cttgaagaaa 24600 caggggcaac agtcttagac actggagcta tcccgaatga ctatacttat cttaatacag 24660 atttcgaaaa ttctgcgcct cttgttgtaa ctcttgtaaa aaattcagtc ctgaaagatg 24720 aacgtggagg tattattgaa cgtgtcattg ttgatggtga attagtttac gaagaaggac 24780 aagagccacc agaatatact atccatctag atgtagactc caaaggggta aaataaatgt 24840 ggaatccaat tgttaatgta gatattacat tgaacaccgc aggaactaca agagaaggtt 24900 ttgggctacc actattttta gcttcaacag ataactttga agaaagagtc cgtggttaca 24960 cttccttaac tgaagttgct gaagatttcg atgaaaactc tgctgcatat aaggctgcta 25020 aacaactttg gagtcagact cctaaagtaa cccagcttta tattggtaga cgtgctatgc 25080 agtacactgt atcaattcct gactctgtta ctgaaagtac cgattattct ttgactgtag 25140 ctgtgagcgg tggaatctct cagccattcc agtatacttc aaagacatca gatactgctg 25200 agaaggtgtt acaacagctt aaaacacaga ttgaagctga cccaacaatt aaagataagg 25260 tcactgtgaa tgtaactggt gctggcggtt cagccacaat ggtaatcacc aaagctggtg 25320 tgaacgactt cgtgaaggtt acttctacag cacaaacagt gtcaattgca agtacaactg 25380 ctgacacagc atctactgct ttggcagcta ttgaagctta ttcaactgac tggtatttca 25440 ttgcagcaga agacagaact caacagtttg tcttagcaat ggcttctgag attcaagctc 25500 gtaagaagat tttctttaca gctaactctg atgtaacagc actgcaaggt acagaattag 25560 ctagtgcaaa tgatgttcca gcacagcttg ctaagagtca gtacactcgt accgtttgct 25620 tgtggcatca cacggcagca gaagactatc cagaaatggc atacattgct tatggtgctc 25680 catatgatgc aggttcgatt gcttggggca atgcacagct aactggtgta gctgcttctc 25740 tacagccagc taataagaga cctctgacaa gtattcagaa gtcagcttta gatgcacgtc 25800 actgtaactt tattgacctt gatggtggtg ttccagtggt tcgtagaggg attacttctg 25860 gtggggaatg gattgatatc atccgtggtg ttgactggtt agaatcagac ctgaaaactt 25920 ctctgagaga cttgctaatt aaccagaagg gtggtaagat tacttatgat gatactggta 25980 ttacccgtat tcgtcaagtc attgaaacct ctctgcaaag agcagtcaac agaaacttcc 26040 tgtcatctta cacagttaat gttcctaaag cctctcaagt tgctttagca gacaagaaag 26100 ctcgtatcct gaaagatgtt accttctcag gtatcttagc aggggctatc ttggatgttg 26160 acttgaaagg tacagtggct tacgaataat agaggtaaat tggaatggct atgtatcagc 26220 aatattcccc taaagacgtt gtatgtagct ggaacggcat tgctattgaa ggctttgctc 26280 cagattcatt cttacgtcta cagagaacat caccacttat cacaccagtt gtaggtgcgg 26340 gtggtcaagt tgctctgaca agaaatgcag acaagacagg tactattgag attgagctaa 26400 tgcagacttc tctctctaat cagatgcttt ctgcaattca agctaaacaa gacaatatgg 26460 aacttgaaga agatatctct tctaacttcg taatctacga cccatcaggc tctgttctgg 26520 caactggtat taatgcttgg ttgcaggaat taccgcaggt tgaacttggt cgtgaccaga 26580 actctaaaac ttggattttt ggttgtgaga agctagacta cacttctaca attccagcgt 26640 caagtgttta ataaatccta taagggggag actttaaaag gtcttcccct tttttgtttc 26700 ttttaaaagt attaaggaat cacaatgaaa acagaatcta gagtaattaa tggtaagaaa 26760 gtaaatatca ttctgcttgg ggcaagagat ggtattaaga tgtctatgaa gttgggtaaa 26820 attgttgttc caacctttgc acagatgcta tcaagtctga ctgataaaga taagaaagaa 26880 gctccaatgg ttccatttaa agaacttgtt gaagcttgtt ttgacagaat tgaagaaatt 26940 aaccttgaag aaatggctaa cttgttattt caaggggcaa ctgttgatga cttcccactt 27000 aatattgata cgtacttcca agcaaactac ggtgagttta ttgattactt agcatttgcg 27060 ctggaggcaa acttcggaag ttttttcgaa gcaagcattt tcaaaagcct aacttctcag 27120 taaacatggg taacactcta cagacaccac taactgatgc tgctgtagag gcaacctatg 27180 aagaagcaga cgagatgaaa tttgtgcttg ctatttatgg tatggaaggg tgtaaagaaa 27240 cacttgacca actctttgct atgacattct ctgatttatt atcattgaga caatttcttg 27300 aaattcagag gtcgtataaa gaggaaattg cttacaacga acttagaaga gcaggaaaaa 27360 tgtaatggca caatatacag ttgatagctt cattgtggaa cttggtttca gtgaaaaggt 27420 aattaaaggc ttgcaaagag ttgagaagat gtctatgcaa gccgctcagc gtattgagcg 27480 aaatatgaat aaagcctttg atgtgaagcc taataaaagt tctcaggaag cacttaatag 27540 aattgtaaaa aatgctcagt ctgcttcggg cagaatcaat aaagcactca acagttcctt 27600 gaaccttgat tcacaaggtg tgaaatctct taagaaactt gaaactcaag caaaaaagac 27660 tgcaaagggt attaacaagt ccttaagaga tgctatgaag gttgacggta aaatcactat 27720 taagacaggt agagggagag gtggacgaaa tatccctcct gttggtggtg gagcacccag 27780 aggtccgaga gtagatgttg ctcagagaca aatggaaaga atgtttaaca acaacttcta 27840 ttcagggtta actcgtagac tggaaacaat tggtggtcaa ggtaaccaga tggcagcttc 27900 tttcagagga agcttacaaa gtatctataa taaatacaag ggtactggta aggttggtga 27960 gtatgagatg gaagttaaaa agctcatcga cgtaaccaaa cgttgggtta ttgcagaaaa 28020 tgctagacta aaatcagtta aagaagcagc ttggctacag gacagagcta actcatcact 28080 acgtcaatta gttggtggat ttgtttcagc ttatgcttta ctggaactat cacaaaagac 28140 tattgaggcg ggtgtaaaaa gacaatctgc acaattagcc tctacagcta tctttggagc 28200 agatacccag caagccagaa tgtttgctgc atcattcgca caccagattg gtcagaacta 28260 tacagatacc atgaagcagt actcaaactt tgctgctggt gctcaaccaa cgctaggttt 28320 tcaaggtact caagagttct ataagaacgc tgcaatgttt gcccgtatca gaggtgctac 28380 cgatgaagac ttgaaaggta tcatggttgc attccagcag atggcatcaa aaggtaaagt 28440 tcaggctgaa gagcttcgtg gacagttagg tgaccgttta gcaggtgctg tgcagctatt 28500 cgctgatgcc attgggaaga ctccacaaga acttgataag atgatgcaag acggtaaact 28560 tcttgctcaa gacgttctgc ctaaagtgtc tgaaagaatg gctgagcttg tcaaacaagc 28620 aggtggtatg aacgccgtat ctaagcagac tgctacgtca atgggtcaag ctaaggcaat 28680 gtgggataac acacttgtag cactgtttaa caattctagt gagggtattt cacagttatc 28740 taactctgtt gcaatgttct tacaaggttc tatggggtct acagaagctt taggtgttgt 28800 gattggtaac ctgttaaaag gtgctagtaa cctacttgac tttgttacag acttcatgta 28860 cagagtctct gcactatact actatgcaag agcttggtat aaagaccttg acagtagtca 28920 gcaaaaacta gtcaaaagtg ctggtgaatt tctgggaaca gtcactatga ttggtggtgc 28980 tgtagcaacg gtagctaaaa tggcaaagct tatcactgga ttctttgata cagcaattgt 29040 gagaaaaatc cttcagaggc ttggtattga ggttgcagaa aaagcagcac cacgagcagc 29100 accattactt gcatctccag ttggtgttgc agcagcaacg cttgcgctat ctaagtcttc 29160 tgaccctaat gctggaaaga gattcaatga agctaatatc actaatcctt tcaatgaagc 29220 tgttgcaaat attacaaacc caaaaagacc aatgttcttt gatgagaatg gtcaacttaa 29280 gtttgcacag tacactcaag atatggaagg taacagaaag ctaattgaca atggtctatc 29340 taattgggat attctcatgg agaagttatc agcatctctt gataattttg ccaataagtt 29400 taatcagaca ccaatgatga tgacaccttc tggtttacct atgcagacta aacaaaccct 29460 gaatgttact ttcaaacttg atggtaaaca aattgctact aagatggtgg atattactga 29520 caagaatcaa gaagacattc ttctaagttc aagctatcca gaggaagaat aatgttatgg 29580 gattctaata tgcaaatcaa atatagtggc aaagatggca tctatttcca cttaagagat 29640 aatgtagatg ccttcttaac cttatcagca actgaaaaca tggaatttga cagccctatg 29700 caggtaacta cacagaacat gcaatcaggg caaaccgtca cagataatgt tcaaagagca 29760 cccagaacaa tcactattag tggtgtcgtt gtagttggct atgaaggaag cttattatta 29820 actcgtcagg gtcaattagt agaaaacttc atcgacactc tggaaaactg gcgtgaccag 29880 aagcagatta tttcggttat ctgtaaagac ggaatcaaga ttgatgattc cattattacg 29940 agctttaaag cctctaaaga tgttggtatt tcaaatggtc taagaatcca actaaccttt 30000 caggaaatta acttcaaagc tgttgtgggg caaactgata tctctgctgc aactggaaag 30060 gccgctacta cgaatgatgg tggtgcaact tctaagaaga atactggcaa cactacgaca 30120 agtttaggca atggtaaatt aaactgccag ttattatttg acctaaacgc caatggtgta 30180 agggaactta ctagtgctga agataaggct cttggaaaat gttcaatgtc tgcaaagaca 30240 agaaaaggtg ttaccacatt tagtgacgag gctgaaaaaa atgccggagc agcgttaaat 30300 aggactgctg gaactggaaa ggcattacaa aagcactcag tgaatccgaa taagaaggag 30360 acttattaat gtcacaatat attcctgttc ctgatacaga atggtctaca caaactgtaa 30420 ctctggatgg cactgtcttt gtaattgagc taaagtataa agaaaggctt gacagatggt 30480 tcttgacgct atctgatgtt gatggtaatg tattattaca tgaaaagaaa tgccttgcag 30540 accaatcaat cacaggacgc tatgtaattc cttcattagc tggagagctt tttgttgaac 30600 gaatgtacgg tactgattta caacctacca gaaataactt cggaagagaa aaggcatttg 30660 agcttaatta ttacactcaa gaagatatga gattaatgga gaacttataa tgtctgtaaa 30720 agatagcact gctggggctt cttttaggtg ctatcaattg gctgtaggta gtgaaactac 30780 agcctttaat gataaaccta caagccatgc taaagattct atgcaaatgg actatttcga 30840 caacttacaa ttcacttgta atgtgtctta tacgtctcag aaaaataaag taacctctga 30900 cgataccact tttgaaatct acaaccttaa taaagagatg agagccaaat tcaaaaccgt 30960 tggtgcaaca gttatgctca gagcaggtta taccactggt tttaaaaggg actcaaacgg 31020 tgaccttatt attgaatatg acaacctccc attaatttat ctaggcacta ttgagtatgc 31080 ttatacctat aagcgtggtg ttgatatgat tacgaaggtt atctgttcca atgataaaat 31140 ggaaagaacc acgattaaga catcaatttc ttataaagca gggacaacac gtaaaagtgt 31200 gattagagat ttagttagca gattaggctt ctcacttatt gatgaagacc tttcaagtat 31260 tgatggttac acttacaaga atggttttag tgtttggggg agtgttgcag aggcacttac 31320 aaaggtttgt gaagaaagta gtctacgttg gtatacattt aacaagcaaa tccgagtagt 31380 cccttttaat gctaaagcta gacaactttc ttgggaaatt tacccatata acgttattga 31440 ctctttgcaa ggttactaca gaagaactag aaaggttctg aaaaaagaga ataagacagt 31500 tattaaagtt aagactggag ttcgttgcaa aatccattta gatgggagaa ttaagatggg 31560 tgataacatc actatcagag aaagtgaaga ttttgaaggt cagtatcgag taaaaggtct 31620 ttctcataat cttgacttta ctggtggtgc ttggacaact gaactagatt tagagaaggt 31680 ggaataatga agtcaccagt tactagaatg tctgggtatg tttcagaatg tcttgatgaa 31740 tttagaaaag aactgtatac tggattacca gctattatcc agtcttttga ttcaaagact 31800 caaacagcca ctgttaagcc actttactct attaacggtt tgcctatgcc agagattact 31860 ggtgtacctg ttcaattccc aagtggtggt ggagcatctt taacattccc cgtaaaaact 31920 aatgacagat gctggttagc tttctcaatg ttacctttag atgacttcgt tgtcaatgac 31980 aagaatgttc agatggaaac aaacatgaga aggacgcacg atatttcaga ctgtgtagct 32040 ttcgtaggca tctgcacaag aacacagaat tttaaaccag acccaacagc agtcagactc 32100 cattttggtg actctgtatt aagaatcact gatgatggta atttctactt tgaaggtgat 32160 gtacacattt ctaaaaactt atatgtaaca gaagaagtac atggttcaga ttttatcagt 32220 gacacgactg gtgtgagctt taatgaacac acgcaccatt attactggac agaccctgct 32280 ggtgaggctg atactacaga ggcacaataa tgaaaacaga ctttgcatta aatctaggtg 32340 gtgactatgt tgccacttta ggttcagatt cagtgtatgt ggctcatggt gatttaaaga 32400 ttactggtaa ccaaattaga attatcccag aggatgataa agctactcag gttgctcaaa 32460 gacttcatat tagatgcctt ctaagggctg gtgaagtctt ctttaataca tctgctgggt 32520 tcccatattt acaacttgcc aaatttaaac agaaaacttc tatctttgat aattatatga 32580 aagcctacct tgttgaaaca agagatgtat ctaacatcta taactattct tcttcaatgg 32640 acaatgctca aagaaaagtg actgttaatt ttgatgcaac cactacaaca gatattttaa 32700 cagacattac gcaagaggtt aatatctaat ggcaggacta actacaacag gattacaaac 32760 tctaagatat caggagattt ttgataatat caaatcaaga cttcttagag atatttcacc 32820 aaaccttgac gtttctgaag acagtcaatt aggactcttc ctagcttcaa ttgcaaggtc 32880 tttagcagat actcatgaga ttctatcaga agtctatgac ggtggaacaa ttgacaaggc 32940 cgagggtttt aaccttgacg atattacagc tttgaacgct gtctacagat atgttgctca 33000 ggcaacaaga ggtcaagttg agtttactgg gactactggt gctaccattc cttcaacaac 33060 aagattaaga agtactgccg gaaacatctt ttatccagtt aataacatca cccttactcc 33120 atcatattgt gttgaagcta cacttgaagt taactcttta cgaactgatg cagattatgt 33180 tattattatt gataacgtca ttttctcata taaaccaacc tcattagaca caatcaccac 33240 gctgctaaca aaattagcag aagctattaa tggtggtatt gtagcaaaag cagaagtcat 33300 taatgatgga gccgcattaa gagtctataa agacgaaggt gatatcattg caagaacaaa 33360 ccctatggtt gtcactgcaa caacgtttct gacctttaca aagattacaa ctatttcaga 33420 tgtagtagct gaagaggtgg gtgctacccc tgctttggct ggtacgctta ttgagattga 33480 gacaactgtt gatggtcttg acagtattta taacagatat gacttaacaa ctggtagaaa 33540 cgaagaaact gacacagaac ttagacagag atatttagag tccttagcag ttactggtgt 33600 gggtactttg gatgcaattg tagctgctgt taaaagagtt caaggtgtct cagatgcttc 33660 aggtgttgaa aatgatactg aagagacaaa ctcagaaggt cttccaccaa aatcttttaa 33720 gattgtcgta gttggtggtc aaaatgataa tgttgctcag gcaatctggg atacaaaacc 33780 tgctggtatt agggcttatg gctcaatttt tggtacagcc catgatttag gtggtttggc 33840 acataatatt tattttagca gaccaacacc aaaatacgca tttgttaagg tgtcttactc 33900 actatacgac gaagaaaact taacaatccc tgaagaggat atcagagaca gcattgtaca 33960 aggtattaat gcctacggaa gaactcttaa ggttggtaat gacgttatcc ctaacagaat 34020 ctatggacat atctatgacg taattaaagg tattgagatt aatgaagtta aagtggcatt 34080 atcaaataac caatcagttc cacctagtga cgggcagtat actacagcaa gaattactgt 34140 tgacggtgac caatatactg tttgggaaag tagccagtat accatcacta aggagtaata 34200 atgtttgaaa agattgatag tgtttattat aaaacacttg atgaaaggac tgtaacacag 34260 tttaaagata aatttatcta tacaagtctc ttgaaagcaa tcactgatga gctacaaaca 34320 ctagaggatg tttgctggca gatgcacaca gagagaaata tcaggacgtc agtaggtcaa 34380 caacttgata atattggctc actgattaaa gttcctagac ctttaggtgc tgatgatgaa 34440 acatacagag ctatgcttta tatccaaatc ttcctaagac gttctgatac tactccaaca 34500 ttcttgcaaa aagcaattat gacattgtat ggtgcaactt tctctcagat ttttgagcat 34560 attactccta agactggtgg tatcgttatc cgtattaata ctagaaaaag cgttcttgat 34620 gctgcatata cactgtcaaa gatttctgca acaaccactg gttccgctgt tatcttaaga 34680 gatgttactt taaatgggac tgcttggaca cctgtagagg ttgctgattc atcacttgct 34740 attgttgatg ataaagagaa ctggtttgta actgatgcta acaggggtct ggtaacaaat 34800 aacactggtg gctctctgga gtccaaccta gtagggagtc ttgcagatgc tggtgttatt 34860 gatggttact ttaaggttga cagaacagca aacccacaga gcgatgatta tatgactgta 34920 gataggtcca ataatagtgg ctatttcatt gctggtaaag aagctattgc aggtggtgca 34980 tatggaatca tggctgaggt tgcacaaatt gtcaaaggca gaaaagataa atcacaacaa 35040 gaaggaaatt cttaatggca gttctaaatt ggtctacaaa cgaagtagat actgatggta 35100 atcagttaaa agttttacca ccaccggaag ttcaagagac aggtctttta aagggtgagc 35160 ctatgggtag acagtggttc aactatatta tcaactacct gctgaataaa gtaaatggca 35220 ctgttggtga agttcgttca tatgcaactg agcaaccaga tttagtggct aatggctgga 35280 atctcattaa gtctgagaca gggactgcat caacaagtac aagaaacctt tacacatatg 35340 aatatgtagg agcttaataa tggcagtagg tgaaattcaa attagtgcct tgccacaagc 35400 ggccttacca gttgatttga gtgatatctt ccaccttaag caaggtgttg aggataaaag 35460 atgtacgctt gagcagttgt tagcacctca cgcatctttg aggaataacc ctcatggtgt 35520 aactaaatca caagtaggtc ttgataatgt tattaactct cttcaattag ttgcagctaa 35580 taacttgtca gatattgtaa gtgttgctga ggcaagagca aatctacaaa ttatgtcgtc 35640 agaggaagtg aataacttaa ttcagcaaca tattgacgac aaaagtaacc cacacaatac 35700 aacgaaagca caggttggtt taagtaatgt tcagaactgg acaacatcta atctgtacaa 35760 tgaagatgct gataagtatg ctaccgcgaa agcagttaat aatttataca aagctatcca 35820 agcgtcatat ccagtaggga ctattcatct atctgttaac tctgctaacc catccactta 35880 cttgctttgt ggaggtactt gggagttagt ttctaaaggt agagcactag ttggttatga 35940 tacagattct agacctgttg gttcaacgtt tggttctcag actgtagcat taacaaataa 36000 taatttacca gcacatactc actctatcta tctaacaggt ggtggtcata cacactctgc 36060 aagtgtatct atctctagtt ttgattatgg ttcaaaaagt acatcaactt ttgattacgg 36120 tacaaaaacg actaatagtg ctggtgctca cactcacacg tttagtggca caacaagtaa 36180 tgctggtaac cacaaccata gagtccctat gaggggtaat gataggggtg gcacaaacgc 36240 tatcactgca tctgctgatg ctggtgttgg taatgctatg tatactgatt tagctggtgc 36300 tcacacccat agcttctcag gtacaactgc atcatctggt gctcatagcc atactgtcgc 36360 aattggtgct cacagccaca ctgttaatat tggttcgcac agtcatacag gcactgttac 36420 tgtttctagc tctgagcata cacactcagg gacatctggt tctgttggtg gtggtcaagc 36480 tttcagtgtt gagcagccat cttttgtagt gtatgtgtgg cagagaacag cttaatttct 36540 ttacaggggc tataaagccc cttttaagag gaaaattcat aatggcagat tacaaattga 36600 gtcaattaaa ctcaatcgac acaattcgtt cagaagacct attgcacatc agagttaaga 36660 aaagacctga aatgttgggt gacgaagacc gtagaatgac ctatcaagac ttcttagctt 36720 ccttcaaact agaaaggttt gttcagattg ctgggagtac tatggttggg gatttaggta 36780 ttgtcaagtt actttacggt ggtaaggcag tatttgaccc aacaggctct tctgagatta 36840 ctattgggga tattttaaag acttttaaaa ttaactcaaa tgggcttaaa ctcactattg 36900 cagatgccac aaggtcggca actgtttatc atactttgaa taaaccaagc cctaatgaac 36960 ttggtatgag aactaatgaa gagaatgatg caagatatgc aaggcttgct gttacaaaca 37020 cattctctgg aactcagaac attcaaggtg atgctaactt acttcgtctt agaaaccaga 37080 atgcaaataa tgcgcaatat attgagggtg tgaacttaga tggttcagct agatggtggg 37140 ttggtattgg tagtaatggc tctgatgaag taaaactgta caataacaaa tacaactcag 37200 ctttgactat tgcaagcaat atctctgtta ataagtcttt agcaatcact ggacaagttc 37260 aaccttcaga tttctctaac ttagatgcta gatacttcac tcagacagtt gctaatcaga 37320 gatttgcaca gttagctggc aataatactt ttacaggtgc taacacattt aggactatga 37380 gtgttatttc aaatgctgct gctatcacac tgcaatgtgc atcagcaaac gaatccctgt 37440 atctacttgc aaaagattat gaaggtggaa acttatggta tcttggtaaa ggtagttcag 37500 gtgctggtgt aactatctac aactatacca ctgacacctc tttggtctta gatgctgctg 37560 gagtatcagc aaataaaaat gtcagaatca ctggacaagt tcaaccttca gattgggcta 37620 acattgactc tagatatatt ccggcagcaa cattaagtac aattgcaaga actaatgcaa 37680 ggaatacttt taatggtgta cagacagttg ttacggataa tgaaggtctg attgttaaaa 37740 actctactca gaatagacca ttatatattc gtggtgtgga cactactaat gtatcaagat 37800 ggtggattgg tgttggtggt gctgatacga atgatgttac cctaaataac agttattctg 37860 gaacccaatt ggttctcgga aatacaacat catacattaa caaaacattg actattgctg 37920 gacaagttca accttcagat ttctctaact tagatgctag atactttacg caaagtgcta 37980 gtgatagcag atacctaaga atcagaagta ctagttttaa tttaggaaac ggtgaaaagt 38040 gggctaaaat cgctactgtt gtaatgccac aatcaacatc tacggcagtc attgaggttt 38100 ttggaggtgc tggttttaat gcgaataacc cataccaagc aggtaagtgt gaaattgttt 38160 tacgaacgtc aaataataat acaaaggatt taaatgctgt agcttggaga acagcagata 38220 atgccctcat cacggatatt ggttatatta acacttctgg tgacacttat gatatttact 38280 gtaaggttgg tggctaccaa aattctacgg tatctagggt tcaatcttct agtaatgcaa 38340 gtgtacagtt gtttgaagtc ccgcaaacat ttgatgatgc tccacaaggt attgtgaagg 38400 gtacacttgc aaggtactac acaagtttgc aaaaaccaac cccttcagat attggtgcat 38460 acactaaagc agagactgac cagaaaattg cagaagcgat tagtgactct acagacctga 38520 ataaaatcta tccagtaggt attgtgacgt ggtttaacag caatgttaac cctaatacag 38580 cacttcctgg gttaacttgg acgtacctga acaatggtgt tggcagaact attagaattg 38640 cagcagcaaa tggttcagat gttgctacaa ctggtggttc agattctgta acgttatctg 38700 ttggtaactt accttcacac acgcacagtt tctctgctac cacttcgacg tttgattatg 38760 gcactaagca cacagatggt cagggtgctc acgaccatga tagaggtaat atggaaattc 38820 atggtgattt tggtttcttc aggagtgaca catcaagctt ctacactgct agtgggtctt 38880 ttgctatctc aggttcacaa gcatctagag gatacacagg taataacttc acttacggta 38940 ctcctgttaa tttctatgcc tctagaactt ggacaggtag aacaagttgg gtaggtgctc 39000 atacacatgg tgtaggtatt ggtgctcata accatacggt aagtggtaac actggtggta 39060 caggttctgg ttcagcattt agtgttacta accagttcta caagttaatg gcttgggtaa 39120 gaactgctta atcccttatt gactgattgt taagatggtg ttaatattct ttatgggtat 39180 tctcaccatc ttggctgatg aggtgattaa atgcctacaa tcctagcaat tcttctaaaa 39240 aatctaggta gcttcttctg gaaactcatt ttatcccttc taagtgaata catgattgag 39300 aaagtgttct ttaggcttgc aagatacctt gcgagtaaaa cagacacacc tattgatgat 39360 gagttcgtag ataacttaga gaaagctttt aagggggaga ataaatgaag tggctagagg 39420 aagcttttaa aaataatatt ggtgcaattg tagttggtat ttttagtgtt attgggatgt 39480 ataccacgat gcaagtttca agtggtaaac aagaagtgtc tgtctcaaca aagttacagc 39540 agctagataa ttattcaaaa agtaattact cagctattcg tgatttacag tctgacatga 39600 gactgctaca gctagggatg gagaaccaga aagtccagtt agagaatgtt aagggtgaga 39660 acgcaaaact tactaagact cttgataaat tctctgatag tgtgaacaat ctggctcaat 39720 cagtatcagc cctccaagct attactgaaa agaacacaaa gaatactgaa aagtaaagtt 39780 taaggctcct ttttacggga gccttttttg ttttatttac ctactgacaa aggtgcttca 39840 atctttcctg catgttggta attgttaata ccaccaataa agtcacttgc agtgagatgt 39900 tttaagtcac ttaaagtgtt aagtgggata ccaatctcaa aagttggtgc atggaactct 39960 tcattgttca tcagttcata aacctgtttc atatggttct gataaatctg agtatctcca 40020 aagacaccta tcaagtaccg tggagtatat ccagtcatct tacaaagaac ttccagaata 40080 aaaccgtaag atgcaatatt aaatggaagt cctaaaaagg tgtctactga acgctgatac 40140 cattgtaagt caacttcacc ctcgttagta atataaatct gaaacagaac atgacaaggt 40200 gctagagcca ttgagtttgc tgcaatatct gccgcattcc aagcattaac aagcatgtaa 40260 cggtctgtga tatcaccctt catcttcgtt actaaggtct ctaactggtc tacaacacaa 40320 ccgttatgac cttcaaagtt tctccactga acaccataaa ttcttccacc cgcatcttct 40380 aaccaatctt gttcagaaga gtaattagag cttaaccagc gtttaaaatc atctgaccag 40440 atagtccagc gttctccatc attctcaccc caagtacggt aacggagttc acccagctta 40500 ttctcaccat tcaggaacca taaagcttcc ccgataactt gccgtgtaaa cacctgttta 40560 gatgttaaga gcgggaaacc agttcgcata tcaaaacgaa actgaggtgg agcaaatgca 40620 gagataacat ctccagttcg tgtagtacgc agttcaccaa ctgataaaac atggttcagg 40680 atatttttgt aactcaaatc tgcttgtgac atatcaaaag tgtcctttta cttgtggaac 40740 ataaatttca aaagttgctt taccatcctc tgatggtttt gactgtacct ttgtaaatac 40800 acggttatca tacagcttct caaagaagtt ttcaaaaggt aaatgcacag tggcctcttc 40860 agtaacttta tggaaaactg tatgaaacac tacatcagca tacggtaaag cattgacaag 40920 gacacctgca ccaccaatta caaagacatc ttcatcaata gagctatcaa gatacttcaa 40980 aaatgctcca aatgactctt tactggcttt agcatacatt acctcatctt taccaaagtc 41040 tatgcctaag tatggtacag agtttgttaa gattaggttt gcacgttctg gtaaaggttt 41100 actacccaga gtcttaaaag tctcattccc catcactact aagttatttt tagtgagtct 41160 cttaaactct tgcatatcct gtttatgtcg aggccacggc ataccagttg gagtaccaaa 41220 ctcaccattt tcaccacttg caaagattaa tttaatcatt ttgtaaaccc tttcttaatg 41280 aaccagttga taacattctt cacagcttta ttgctatggt agattaggtg tggtgtaggc 41340 tggtatgaac cataggccca gaatgataga tgctcaccac aaccaacaca tgtagtactt 41400 tcttcagcta tatgaccatt aacaatcaca tgaacttcac cttgcaagtc tgtacaccca 41460 caatgtgggc aacggatagg tgttccatca gactcatagc agtgttcaat tttcatttat 41520 ttaacctcaa aaactggctc aagaaccaat ataaagtcta cacaatcatc ttcttcatcc 41580 agaataccat caaagttctt aggaacgaag tcgaagaggc tcataacaaa acagtcagaa 41640 gtaaccctga tagttcctcc catgttctct acagtcaaca ctgtacgact ttcttttacc 41700 tctctcagaa gattactcac aaagtcatct aagaagtact tcatcagtga cttaaaacct 41760 ttagggtaat ctgtaccaga gtatgtttca atctggggta caagcttaac atttatacta 41820 gtctttgtta agaacgtgct agagaacatg ctatcaggta acttacaaac aatctttcca 41880 gtaccttcat gctcaatctc aacaaaacca gcttcgtcgc cacatgtaac tattttaaaa 41940 gatgcttcat tctcatgctg tgaagccata aactttttaa gcatcacttt aaggaaatct 42000 gcatgagcat cttgtaagta gatatcaatc atagttaatc acctattatg ggaacagaca 42060 cttgatgtca tcatttttga aaacaaaact tgtgtagttg tgaccatcaa catccagcca 42120 aacagcctga acatctacac catcaagatt gtggtaaagc tctttgatga agtggtcttt 42180 cacaacctga ctactagttg tttccagctt aatgtttgag attgctgaac ctacagccat 42240 ataacccatg acacgtttct tctcagtatg cttaatctta ccatctttgt aagccataat 42300 gagattggaa agaatcttca caccataata cttttcaagg tgagtaggta caccgtaaaa 42360 acctaatgcg aagttttcag agtggataat gttttcacgt ttcatattaa tctgccaacc 42420 ttaaattaaa attaacacca aatttttcac atacttccaa cataaactca agagagacat 42480 taccagtgag gttgattatg ttggatacac gagcttgaga gataccacaa atcttagcaa 42540 cttgctcttg agaaagacct ctggatttaa tctctctctt aaaacgatgt gcaacgaagt 42600 ctctcatttc gtctacatcc attggacaaa tataactctc catttcagct tctctgtcag 42660 cctcccaatc atcttgtgga gcataagggt caaaaacctc attcatcttt cttcttccgt 42720 ttacgtgaag catcaatcaa tgaatctatg tttgtagctc taaccttttc acgttcatcg 42780 ttaagataat catcaagagc cttctcaatc tgtgaatcat cttgacagat aactgtaaag 42840 actgtaaagc catctatatc atcaattatc ttaccataac cgcttctggg atgtaacttt 42900 ttaagagcca tatcaaatac ctgtgcagct tctgttttct aagaaatact caacaccata 42960 catagctttt tgcatcaagt cttctttaaa ctgaatacga tgattgagca tagcctgtac 43020 atcaatatct tcatggtgct tctctttaat gtttgcagat tgcaggaaat actctgcaag 43080 aacacgatac tgatagtcag ttaccttatc agtcatctct gttaaagact cttcagagat 43140 tggtttaagt aactcaccat tagccaaagc aacaccttta acaatctgac aagtgtcttc 43200 tggaacacct gataagtcca catttgggag ttctgttgca ttgactggta aattcattga 43260 cataaccatt gcagcagcta ataaaatctt tttcataatg acctcaaagt tatattaaat 43320 tttgtattac attgagtata aaaataagct ataacacctg atttgtcaac agattttata 43380 gcttatttgg cagatttttt ttataaggct actgccatcc ttccatacca aaccatacca 43440 gacatagcca tacccgaccg tacctcgcct cacaacactt aaaaatcctc ttagaagact 43500 cttaagtgat gtaatcgaat tttaatgtag ctccgattaa ctacccatac ctcacctcat 43560 ccaaccatac catgccttac cagaccgcac cttaccttat taaaagaccc tatttaaaga 43620 ccttttaaaa aggtgagtct cattttaaat gtagctgaga ctaactaccc atactgtacc 43680 gcacctgacc cgacctgacc agacctaaca ttaccgtgcc acaccgtacc gaacagaaca 43740 ttgccatacc tcgcatcact taaaaaccct tttaaaagac tcttaagtga tgctggtgag 43800 tacccgtcgg tttatgctgc tcagtatact caccaaattt tctatgtcaa caacttttat 43860 tcataatccc taataactag tccgacaggg aactgtaaag aacccttacg agtcatcttc 43920 tggaactgta ccgttaaagg cttcccgata aactcttcag gatgctcagc aagatactgt 43980 ttcttctcat gagtagtctt ccatgagaca tctacaaaga cgttaggaag agtctctaca 44040 acgaacttgc catgaccacg cttatcagtc tttacaccag tcactttaaa ctcttcagtg 44100 tgcatcttct tgtgcttaat caagaagtat gaacggtgac aacactcata gaaggagtct 44160 tcagagattg aacggtacat tgcaccttca aacttagctt caacccactt atcatgagct 44220 tcatcaaact cttcccaaga atttacacga cgagacttaa caggaacaac tttacagccg 44280 tctctgaagt cattcaatgg tgaagtctca ataatatctc tacgctcagg ccacgtctta 44340 gtactatcac agatatcgta ccagtaaaac ttgagcaagt gtctgtcagg attgtcttca 44400 tttttaatca tggagacgat atcttctaag tcccacccat gagcataaat ctctccatca 44460 aattcatcaa catctttgtg gagccttagc attgccataa tgtcaggtat tagttcggca 44520 gggattttat aaacagtatt ttcacgagaa tatgctgtaa atacactgcc aatacttaag 44580 tgtcttgaaa ttctacaacg gacaccatca agttttggtt gagcatcagc agggaacttc 44640 agatactttg catgactaac ctttgcagca tcatgagcaa gctgcacacc taccttctca 44700 gtattctgtg cagactcttt tgtgtaagca tagcctttgc ggtcaacctg ctttttatac 44760 ttagcagcaa cttcaaagag agcttgctgt tcagcgttac gctcattctt tttaccgatg 44820 tttttaggtt ctgctgtata ctcttcaaac atcatcttgc cattttcttt gccatacgtg 44880 gtaacaactt tgtcacctac agcgacacat gaccagacgt taaaagagcc atctttattt 44940 tgtttgtaca gtattgtcat tttattcctc tgagtgatac caagtattgc aagcattaca 45000 tgcgtaaagc tctaagtcat ttttatctgg tttatgaaaa cagtttgctc cgcagtggca 45060 acgaaacggc tttccaccaa ctgtcaggat gaaggattga acctttccat caggagtccg 45120 tacagcattt ttatgcttgt cttctttatt catttgtgct attgcctttg tcagatttca 45180 tataggacat gttactatgc cttttaataa aatgtacaac gcattttggt gtatcttttg 45240 tgtctttgat gtgcaatcta tcgaaactgt gtactgtgaa ggttgcatct gtcattgtct 45300 gcttaggttt cattttcttt taccaccaat ctctacgtca ataacttgaa catcaccagc 45360 aacatcaaac ttatgaatga tgctttccac agtgtaagtc caaccctcaa tgcgaaccaa 45420 ctcaccttct cgtgggacga ttgggttacg ttgagctacg gttgtagtgg agtgaacaac 45480 atcataacag tagatatagt ttactaggac ttccatatct tcaccttaaa agaataagtc 45540 agatgctttt gggttgatta aactcatgca gttttgaagc atggcttcat tgtacccatc 45600 ttggcaaact acgtagagtt tatcgaaagt ttcttttgat gctgggtaga ctgataactc 45660 ctggcataca ctaagatacc attagtaatc taagcctctg ctccaagcgt cagcgattag 45720 ggctgtagct ttgtctttgc ttctgataac ttcatcagac ttttcaattt tactgttcac 45780 gatgttctac ctctctgtac gttcgtcgat agtatatgat aagctgtgct aactcgttgc 45840 ttttaacatc tgaacgtagc agttgccaat caaaaccttt accgactctt ccatataagc 45900 taaataattt tactttgcgt ttcattttgg tactcccaat actcagaggc caatactaat 45960 acgttcaccc ttgaggactc ctgtacggtc tcttgcaatc ttgccgtcaa caataaggat 46020 atgcgcagac cttaaagatt cactattaaa accttttacg aacttgctag gcatcactac 46080 aatgtacatc ttctgaatca actctggagt gtagcagtta tcatgaagtt gctgaataac 46140 gtctgcaagt gtcatttttg atgtctccaa tattcaatat ctacaagttc ttgtgcaatg 46200 cttttcagat tacctttcaa gaaaaccttc tcagaacctg aaagagatga attgtcaatt 46260 aacctgttca tcctctcaaa caacttctgt ttacggttct tgatagtttc ctttgaagca 46320 cctttcatca tcgctgcatg ggttcttcca cggtggctca taagccctcc tatataagat 46380 attgtataag gtactcttcc ctgagcacct tgtcaactta gttttgcaaa ctctccgtgg 46440 agtttcacag cagcttcaca gtaagcttta tgggcttctt caggtgtgtc atggtgacct 46500 aaaaacatct tcttaccttt ataacttatc tgtgcgaccc acttaccaga cttcttctta 46560 aggcttacac ccttgtaccc agatttattg gtggctagtt tacctttatt ccaagcactt 46620 tggaagtcat ctgcttccct taagtttgca atcctgttgt caagagagtc tccgttgata 46680 tggtcaatga taccttttgg catcttgcca tatacataaa gccatgcgag tctgtgtgct 46740 ttatgtgctt tcttgtcaaa atagatacta atctgtttat gaccagttga atcagtctgg 46800 atattacctg ccacctttcc tgcataatta ctgttaaaaa cactaacagc tcttgattta 46860 ccttctctac gaagccaagt aaagacccct gtttcggggt catagtgcag cagctccttt 46920 aaacgttttt gtaaaattaa atccattaat caatgtacct ccaaccagct cttaccaacc 46980 tttgctgtac cagttacaag tgtttctttc ctcaaaccta agttttttga agcttgtcca 47040 tacatccagt cagcaattgc tttcatatcc tgtgtcatac cctctggaca ttcccaacta 47100 ttttcatcgt gatatgccag aagtaacctt gctccgatag ctggttttcg agcattcagt 47160 tttgtcaggc catcttcaca ggcttttctt gctgctaggt tgatagcctc attctgaatc 47220 tgtgcttccg aactcatcag gaggtagttc agtagtttat gtggagattt gcaccatatc 47280 catgctccag cgaccctgat ataaccacct ttagcaattg aagaattctt cccaaaaacc 47340 tcttccagag cctttttaga cgccttaaaa tcggcttcta agctatcaag taacttctta 47400 atctttggca gacgcatata gtaagtttgt ttagtcaatg caccctcttc cgtagatgat 47460 gccttgatag tttttgcaaa cttctcgtct ccggctccaa ataacagggc gtagatgcca 47520 tttttgctct tcttacgacc ttttgtaatc tcgtgaagaa gttcctcatc ctgtgtctcc 47580 ctgcaacgaa caatatcctc ttctttgtta agaccaaagt aaatactgtt cagtgtatgc 47640 gcatcagtcc ctgtgtagac ttcatagagg tcattttcag catcataacg cagatacttg 47700 tcagtttctg ggttaagata cttgtcaaga tgtttacagt agtatctacc atcatcttgc 47760 ttagtgaact ccactgactc tttaccctca gttaccgctt tagtgaagtc ttcatcaccc 47820 ataaagttac aaaggagaac tagctgggct gagttttggt caacagaaac aatgttagtc 47880 ccctcttcac aaatccacac tgccctcata ggtgctccgt agacagcagc accagacggt 47940 acgtttacaa taccatactg tgtcatacgt ccagttgaag taccaaacac cattgcacca 48000 gcactaaggc gaccatcagg acgaatctgg ttcaaccaac ctttttcatc atcctttgag 48060 ttctcaatag ttctgcgtcg gtgcatcaaa gtatagtact ttgcaatctt ctgtccaagt 48120 tcaccttcaa tcgtatcata agatgactca gtaagctttg gtgaagtacg gattaagcaa 48180 ggctcaagca aatctgtgta cttcttcaca gaccagttat gctcaatgta ctgtatacct 48240 tcatgttcaa cataactcaa accacaccgc tcaaccattt cctgccactt aggatgtttt 48300 gtaatcatct ttttattgtc tttgaaacga caaactttga cagggcgacc gtctgagtct 48360 ttcttgtagt tccagtcatc tggaatccaa ccaactgatt tcaagtagtc tttaacaacg 48420 gctacctgag tcatacgaga aacttcaaac tcaattggtg tatatggtgc atcaatcaaa 48480 cctgtgtagc gacttgactc aagttcaaag tggttaacaa cgtgactgtt atagtacttc 48540 acagtcttga ctgtttttac tggtttccag tctttgcatt tcttaccaat ctttgcgttc 48600 aactcattgc aaattgcacg agcatccttc attgctacaa aaccttcctt gtactcttca 48660 ccagttacag tgttggttgg tgtatagcaa tttctctttt caatgttaaa aatctttgta 48720 gttggcttac caaatggttt aatttcgtat gtctgcatct caccattacg tacctgctga 48780 cgatactttg tcttagggta cttggtaatt ctcttcagtc catctgcatg accaaatgtt 48840 tcaacatact cattccaagc ttttgcaaac tcttctccag tgactttacc tttggtctta 48900 atagttggag gcaggtgtgg ttcaacctct gaagcaagct cattagtcaa cttgtcaagt 48960 tcctttacat ggaactccat aagctcttta tcagctttcc agccattgat agcttgttga 49020 ctcatccaga aagatgtttc tttagctcgc atataggttt cataagtgtc tatgccacac 49080 ttctttagct tcagatattc attatccagt gcgcgtttag ttttggcgtt aatacggata 49140 tcttctacaa cacgagtgaa gatttctgca ttccacacac cccaatgttc aatctctggt 49200 ttacggacac caacacgagc accccatgca gccaaaccat gagcaccttt ataacccttt 49260 ggagttggtc tatccatcca ctgaacacga gactggataa gagaatcctg aaagaaatta 49320 ctccacggct tgcactttgg attatcaaag ttccataaat caggtgcaat gtgattgaaa 49380 acccaccagt cataacccag accattatgg atgcaaagtc gttttgcctt taacgcaaac 49440 tcaatacctt cacgtaagcc acccttgatg tacttggtat acttgtgacc gaggataggt 49500 tcatctgtaa agacccatac aggtggctct tcatcgtcag atttatagtc tgcgaaagcc 49560 attacgtgca ctttagtgaa ctcaaggagt aaaccatcag tttctgtatc accaaccaaa 49620 tgtaagtttt taaaatctac gttttccatt tttactccct ctaaaatcat taacctcata 49680 cattactgca aaaaattata aaaagcaagt tgacaatgca cttgacaagg tgttaatctt 49740 tgcgaagtgg gtttttactt aaaaggttac ttaacagttt aaatatacag atactttaaa 49800 actcttaaaa gattatttaa tagcttttaa aaagctttaa agtataacgt atagaatacg 49860 ttaagataaa agattaaaac ttaatagtta cttaaaagag gcttacatgc agaaaatgtt 49920 tgttcaccca gacatagctg actttgttag agctttgcag aagttggaag aacttgacat 49980 tgctgctcaa cgggaatatg ctcaccatca caggagaatg gttgatatac aacatgaaat 50040 agagttgtct gaaaactatg aagatgactt aaaatgctct gtatttgagc atatgaaaga 50100 tgttgctaca gcgagacgta aagctaaaga cacagttgct ttactagatt ctcttaaaaa 50160 gaggttgcaa agtggtacag acctgtgtaa tctagcatca ctgattaacg atgttgaaac 50220 ttcttgggat aggcactatt atccacgttc tgaaaagaca cttgactttt cttcatccga 50280 aaacttaaaa tgttctagaa agaaactcaa ccaactgaga gagaaataat atgaatatca 50340 atgtagaaaa gatgcttgaa gaaaaaggta ttgacgaagc gttcttaatc aatgccgtag 50400 aaagcatggc tatgattctt cgtggcaaca actacacaga acagtttatg cctgcaagct 50460 ataacttgcc taaagataag tctgataacg gtgcttttgc agaacaggtt aatatgatgc 50520 tgtgtgataa aacacttgct ttagcatcta tcgttgtagg tgttgatgct cttgcagagt 50580 tcatttttta tgagatgcag aaaaatgact atattgccat gacctctgaa gaaaaagact 50640 cgctactgct cttaggttat gatgagctag ttaacctgtt aatttcgtca acgagtgctg 50700 ttatggctgc tcttgagaag cgaatggaag agaagctgtc acgatatgaa aagcttgatt 50760 ggggtgtaga agagttaaac ccacgaatca aagagattat ccagcaaacc aaagattctt 50820 tggaagaagc tgtgaaaaat gattctgtag ctgacataga gccacttcag gtgaaagttg 50880 cattgatgat tccagccatc tccacgatga tgaatatcgt tgcactaatc cagctttcac 50940 agatgatggg cacaagcatt gagttcattg agagtatgac tggtggtgtg agtatgcaag 51000 caatcaatga tgttctggtg agcattggtg cttcaatcat tgaagagaag ctgcgcatta 51060 actttggtga tgattttgtg aagcaaatct cagctatggc tgaaaaagag tattcacagg 51120 ctaatactga ccaactgcac taagtcttta taaattttac aagcctcctt agttgggggc 51180 tttttagtaa ttaagggaga taaaatgagt aatattatcg catttactgg aaaggcacgt 51240 tctggaaaag acacctcatg ttctattgtg aagaatatct tagaggatga gtacggctac 51300 aatgttgcag taatggccta tgcagacaac cttaaactct ctgcttcaaa gatatttgac 51360 ctgacttgga atgacctgta cggtgaaact aaagagactc cacaggtttt tgatttatca 51420 tactctgaac ttatgcctaa agttacggag gctatcgagt tcaccttcag agatgaacgt 51480 tatcatatgg actttaagct tatgtcagag ttaaccggac gattagtcat ggagcttaag 51540 aaggtcgcta aaccgactct attgacacgt ctagggttta gtaaaaagta taaattctca 51600 tcaaggcaaa ttcaacagat ttggggtact gaagtcatcc gtaaagttat gggtgacaaa 51660 ttctgggcta aagaccttga aaaacgaatg gctcgcttct ttgaatcttg ttcacttatg 51720 aatcaagagg gtgttgtttt aatcagtgac ttaaggtttg actctgaagc ggagtggctg 51780 agtagatttg cacaccagac tattgaagta aaaagagaca atgtggataa ggtttcatca 51840 catgtttcag aaaatgggat ttctaccaaa tatgcgcgtg acattatcca taataatggt 51900 actcttgcag accttgaaag taagctaaga gctatcctta aaatttaaaa gagagcatga 51960 agatgagagt aaaagataat ttcaaagtta ttgaccatcg tttagtggaa ctctcatctc 52020 tttccaatga ggtaatgatt gaacgtctta agagagttga atcacgaagg aaagagattg 52080 cagatgaaat ccatgaactg gacaagattg aaaatggatt gaaggcagaa ctacaacgaa 52140 gaggtgctaa tgtctaaagg tcgtaaattg aaagaggctg gtcagtttgt tggtcattgt 52200 gcatgtccac gttgtggttc atcagatgct ggttcaatct accaccatga cgatgattct 52260 tattcgatga cttgctttag ctgtaacaaa ggtttcccag agtgggattt tgataaagga 52320 cagattgtga gcacttattc tactggttca gataataaaa accgtacttt ccgtgggatg 52380 gatttagacg atgtaaaaga aaaccttgaa gcaatggact tgaaagacag gaagattcct 52440 gcaaaagttc ttgaacgctt aggtatcaag gtcgacattg acagtgatgg tgaaattgat 52500 gcacatttct atccgactta caaacgtaat gaagatggca agctagaaca tgttggctac 52560 cgtgttcgtc accgttaccc agaagaccat ccaaaagaac acctacgtgg taagttaaaa 52620 gacttttcag gtggtgttgg agacattaaa ggtgaactgg caatgttcgg ttcatggatt 52680 gctccagaag gtggcaaccg tctattcatc tgggaaggtg agatggagtg tgctacagca 52740 atctacatga cttctctggc aattaaagac aagtctcgtc gtaagaacta ctgtcacgta 52800 tctgttccat caggtgcaaa cattaagtct atcaaagaca actatcagta cattacatca 52860 tttgatgaga tttacttgtg ctttgataac gatgaagcag gtgctaaagc aacaaaagag 52920 gctgctggca tcctacctat tgagaaggtt cgtttattcc agtatccaga aggtgtaaaa 52980 gaccttaatg aatggtggac aaagttctat aaagagaaag acacagttct ggaaggattt 53040 aagcagcgta tctacaatgc acctcgttat tgccctgctg gtatcaagaa ctttgcagat 53100 ggttttgagg caatgaagaa tcgtggtcag attccattga ttcctttccc agaatctttc 53160 ggggatttga acagactaac ttacggtggt tatggtttag gagagattac aactctggca 53220 gcaccatctt cagtaggtaa atcagcttac actcgtgaga tgatttattc agcttggaag 53280 gaaaccgatt acaatattgg tgtaattccg gtagaagata cttacgaaga acttatggaa 53340 atgctctgtg caatccacct gagcaagcag atttctgaga ttccttatga tgaacgtgat 53400 tgggatgaac taaagagagc acacgcagaa ctatctaaag gtcgtcgtat ccatatcgtt 53460 gaccatcaag gggcaattga ccaagataac ctgctggaat ttgttgacta tcttgttaat 53520 agtttagact gtaagattat cattcttgac cctattacgt tggctctgtc acgttcagat 53580 actgatgaag aggaagtttt gtctgagcta ttgcgtcgtt gcaaacgcta ccagtatgca 53640 caggtaaacg tctgtcacgt tcgtaagagt gcaggtggtc agaaggctaa ctctgaaggt 53700 ggggatatct ctgaagagga tattaaaggt tctggtgcgt atttccagat ttctatgaac 53760 aacatcctgt taatgcgtaa caaggttgac ccagaccctg ttaagaaaaa cttgacaaaa 53820 atcaagctaa ctaaatgtcg tcggcatggt aagtcaacgg gtattgctgg tcatacttgg 53880 tacaatccag atacaggacg cctcatcaaa gcatctggtt gtggagttga cattgatggt 53940 gcagcagaaa atattcgcca acagtttggt attggtgaag tggaagacca ttacgatgac 54000 tctttgccac attatgagga tgaagtgttt gaccgtgaga ctggtgaagt ctatactgaa 54060 gagcagcgtc aaagctcaac aattccacct gtattaagtg aggatgcaga tgactgccca 54120 ttcgaaactg agtgatagtt ataaaagaga ggggttcata cccttctttg aagaaaaaag 54180 tttaaataaa tttcaggaag agtgtttgac aaagattgat aggttctata gaatgctcct 54240 catgaaatgt gatggggagg ttgctataaa agaagattcc taccaaagca cgattacagt 54300 catcattaca ctgcctcagc ataatattag ttggctgttt ataattaaag aaaatgtgtt 54360 tgaatatcaa gtatatcgga gaataccatg aaacactcta aagcatttga aaaagttttt 54420 ggggattcct taaaagccac tgctggaaaa ccagcaaaat actatgaaga aaaacgtgta 54480 agaactggaa aaactgcacg taaagcagct tctaaagata agcacaactt ccagtaatta 54540 gtatttgaca atagagtatc aacaaactag aattggtact cttcataaat tgagatagag 54600 gtttaaataa tgtctaaagt tgttaaaatg aaagctccgg tagagaaata caatggtact 54660 gaacgccaaa ctctgcgata cctgctgaaa gatgtctggt tttattacct gaacacttca 54720 ccacgtccgg gaaaaggtaa gtctattgat aagaaattcc caggcaaaga ctgtaactac 54780 agcgtatcaa ttctggcaga agacggtaac aagctgttta aagaatttac taagtctaag 54840 aaaaacccag aaggttggga taaagttact actgaagcag ttgatgcaga tgacttcgaa 54900 gagaagtttg gttgcaaacc accatttgaa gcagatactt actacatctt gaaagtaagt 54960 cgtgcagcag cttataaaga tggtgctgta tggacagcta aacagtcatt ccctgtaatg 55020 ctgattgaag aagtaaatgg taagcgtgta gctgttaagc agccgatgaa gaaaatcaaa 55080 gctcaagcat ctgataaaca tgaagatgac aagaactatg atgtaattca tccagatatt 55140 gcagttggta acggttcttt tggtagcgtg attctttcta ctcacttcta cacttttgag 55200 aacaatgttc tgacaaaacc tattcaggaa cagtttatca ttgatactct tgtaccttac 55260 actggtggta acggtgctaa cggagaacct gaactggatg aagacgaact ggctatgctt 55320 ggtcttgatg gtgtcgaaga taacggtgaa atcactgaag aagacgcaac agaccataaa 55380 tcttcgaatg attctgatga tggtgatgat gaagatttgc cagacccaga tgacgaagaa 55440 gatgaagact tcgatacaga agattaatct ctaaagttac tttaaagccc tgtactccgt 55500 atggggcttt ttcatatgga gagccataat ggagaagtac acattaacaa aacttcccga 55560 ttcagttaca catgttttta ttgactctga cagtattgcc tataaaggtg cttgtgtagt 55620 tgagaaagca aagtataaat atgtcaataa actcacagca gaagagtctg agccatttga 55680 taatgcgaaa gatgctgcaa gatggttagc agaccagaga atccttgtgg aagagcttgg 55740 cctgacattt gatgaagatg aatgggaaag acagacttgg aaagaagcta agagtgaaaa 55800 agaagctatc atggctactc aacaggtgct tcaggaatgg cttaaggttg ttggtaaaga 55860 gagaacttgg gtaggttact taacagagaa aggtgtgcat aaacacaaag acattaaagg 55920 tcttgagcac caatatcaag gtaaccgcaa agatgctgtc acaccaacac acttagttgc 55980 ttgtcgtgag tatcttctgt caagaccaga gttcaagctg attcttgatg gatttgaagc 56040 tgactctatc gtaattgcta aagctgaaaa gatgggtaaa aaggctgcct taatgagtat 56100 tgataaagac cttcgacaag ctgaaggtac ttactgcatt gacatgacct atgaaaaatc 56160 acctttaatt tttattgctg ataacaatgt tggtgaaatc tgggattgtc caataaaatc 56220 gacaccaaaa gctaagaaga cagttggggt aggttttaaa tttctttgtt accaagctgt 56280 ggctggtgat aatgctgata attattttgg tttaaagggc gttggtaagg ctactgtaat 56340 gaaagccctt gaagggaaaa caacttacaa agagtgtctt gatgctatct atgaacttta 56400 tgctaagaaa gagtcataca cttatgtctc gtgggatggt caaacaatta ccagaacacc 56460 tttagagtta atgcagcaac actttttctt agcttatcag gaaagaaaca agaaagatga 56520 tttcactttt aagaaatata attgggagcc ttgtatgaat gaagattaaa ggatttggta 56580 attgccccac ctatggtcat tgggtatctt tatgtgggga agttgacccc actaaacatt 56640 ttggttttgt ctatttagta cactgtaaga aaaccggaca atattatata ggaaggaaac 56700 agtttaacag tgttaccaaa agaaaagttg ctggaaagac tcggaagaag gtagtcacta 56760 aggagagtga ttggatgact tatgagactt cttctgagta tattaaaaaa gatattgaga 56820 gctttggaaa agaatttttt gacttttaca tcattcaaac ctactacaca aaaggtggcc 56880 tagtctatgg tgaagcaaac cttcaacata aattcgatgt aatgacaaaa aggattgact 56940 cgaagctcag actcttctac aatgccaata ttgcagcaat taagtttatt actaaagaaa 57000 cttatgaaga tgctgaaaaa agaatccata aggtaatgaa agcaaattgt gcttgataac 57060 tattgagaga gaataaaatg tttaacaaaa gtaaagctgt gagccatgta gcaaaagttg 57120 acagcaagat tgaagaactt gagcgcattc ttgcaaatgc cagagagtca ataattaaag 57180 aagttgaagc tgttgaatct caaatgcagc acctaatgct taaacgtcaa gagttacgtg 57240 agcatctgga atatattgaa acacgagagc taaaagttaa cagattttta aggagccaaa 57300 atgtttaact caagagagtc agtaaaaaac tggaaccttc gttgtggaaa cactcaaaag 57360 caaccttaca gtgatgagta ttgggaatct ttaaaatctc aatctctgtg tatgcttgaa 57420 gaagcaaaag agcttgtaaa agcaattgaa gagaaagacc ccattgagac actggatgct 57480 caggcagatt tgcaatatgt tcttgatggc ttgatttatc tgtcacaaca tgaccataat 57540 ggcgctatga aagctgtttg ccataataat gacctgaagt acacagacga ctatgaagaa 57600 gctttaaaac gtcttgcaga tattgagaag cgtactggtc aagagtgtat catcagaatg 57660 tcagtagttg atggtaaaga atggtatgca attgttcgtg cagccgatgg gaagattatg 57720 aagcaatcta atctccctaa agttcagctt ggtgaataca ttgtagaact tgaaagccaa 57780 gaactttttg tggtagtatc tgacacatgc gttatctgca aaggtattgt atgtagctta 57840 aaggatttgg gtgtagatgg ttttgtagaa gttaatccaa ttacctctaa agcagataaa 57900 gatttctgta aagagaatgg actatggatt gcagatattg tctactatga tggtgagcag 57960 ttccatgtaa cctcataccc gaaactgaat tatgatgcta ataacctgaa gtgctggtta 58020 aaaggggttg gttataatgg attcacagaa cattaataaa gaaggtgtgg ctcagaagag 58080 ccacttctca aactacaata tttctatgac ggtgtttatg aatgacccgt tacttgagaa 58140 gtatggtgag actccagata cacttctgga taatgaacaa gttttaaaag cagtcctgta 58200 caaatacggg attgatattg agaaagagta ttcttttgaa atctgtcaac acaggaatac 58260 ttttggtaaa gttgtaatgg ctccactctt catgggtgta gaaaggactg actatggttg 58320 gttatatcta aaaagaaact tggagaaata ccgtgtctaa agcaaaaaag ttatcttatg 58380 atgacattat ctcaggtgct aaattaggtg ttgatagtat cggacaagat gttaagcacg 58440 gagacacagt tatgtactgt gacgaccgaa gaggcagaag tccaatcttg tttggaagag 58500 ttgtttgcaa gatgcgaggt aattatgttg tttcagatat ggatgtcaac gtcgcacaaa 58560 agctcaaaac aatcatggat agcaagattt catcatggct cttaaattgt atgcacgcat 58620 cgtcaatcac aaaggtaagt gataagtttt acgatatgtg gcagaatgag caaattttca 58680 agatttaaac tagggagcct cttcggaggc tcttttcatc tgtaggattt aaaaatgatt 58740 aagacaatta aaaaatcaaa cggtacagta gtaagctttg acccagaaag actgaataaa 58800 tgggcatcat gggcagataa gcgtggaatt atctggtcag aagtcactat ggaagctatg 58860 aaacgtgtct atgaaggttg caccacaaaa gagatgcacc aagccatgat tgatgtttgt 58920 gttgataagc aaactcaaga gtactcagat atggctggac ggctacttct gggaattatc 58980 tacaaagaag cctttggagg ttttactaag gttcctacgc tggttacctt cgttaaaaat 59040 atggagagag caggactttg ggagaagatg gattattcac aggaagagct tgaatacctg 59100 caaggttaca tcgtgcactc aaaagatatc tcttacggtt atgcagtctt gaaacagttc 59160 agagacaagt atggtatccg tgatattaaa actggaaggc tttttgagtc acctcaattt 59220 atgtttatgg gtatggctat gaaagccttt gagaagcaac caaagcaccg tagactgcaa 59280 gatgttatca agctgtacac ttacctatct gacctgaaga ttaacgctcc tacgccttat 59340 ttaaacggtt taagagcaac taaatcaggc tatgcgtcat gctgtttgat taaggcaaat 59400 gatactgctg aatcacttgg tattgctgca aaggttgctt atgacatgac tacaaagcaa 59460 gctggtattg gtatgttgat ggagactcgc actattggtg atggtatccg tcaaaatact 59520 attgagcaca tgggtaagct accttattac aagcttgtac gttcatctgt agaggcaaac 59580 aaacagaaga gtcgtggtgg ttcagctaat aacttctaca ctgctctaga cccgcagatt 59640 gaagatttac tgcgtttgaa gcaccctaca acggttcctt ctaaacgtat taatgagatg 59700 gactactcat tcggtacaaa cgattatttc tggcagtgtg ttcagtatga tacagattgg 59760 ttgctattct cttacaaaga tgcaccaaaa ctctatgaca tgttctacac agcatctgct 59820 gatgagtttg ctatggcagt tggtcatgca gtacattcag gtgttaaaca cagacgagtc 59880 aaggctcgtg aaattgctaa gctgtttatt caacagcgtt atgctacagg tcgtgtgtat 59940 ccattcttca ctaacaatgc aaacacacat acaccattta aagagccttt gaagatgtca 60000 aatctttgca tggaaattgt gttgccagtg tacggctttg agaaagagac agacctttac 60060 agagacgatg ctgtgaagga ggatggtgag gtagctcttt gcttcctagc tagtttggtt 60120 gcagggagaa tttcagaaga tgaatacgct gacgttgctt attatgctct tgcaatggtt 60180 gactccgtta ttgaccttat ggattatccg tatccgtcga tgcgtaacca tgttcagaag 60240 cgtcgctctg ttggcattgg ccttacaaat gtggctcatt accttgcgaa gaactacgtg 60300 aactactctt cacgagcagg taaaacgaaa cttcatgaac ttgcagagat gcactcttac 60360 tatctgcatg aagcctctct gagacttgct aaagagcgtg gcgttccaga gtacatgaag 60420 ttcactaagt atcctgaagg ctgggttcct ccaaagacag ctaacaagaa gattgatgaa 60480 aagcatgatg caaaactacg atatgattgg gatgacttag cacaacgtat caaagaaaat 60540 ggtggaatcc gaaactctgt attagaagct tacatgccta atgagagttc ttcactagca 60600 actaatacga caaatggctt gtacccaatt cgtgacttta ttttaactaa aaagtctgca 60660 actggtaacg tactgtttat tgttccagat tatgaagaac tgaagtatgt ctatgaaatt 60720 gcttgggata ttgacacctt tgatatgatt gattgttatg caattgttca aaagttcact 60780 ggtcaggcta tctcttcaga cttctatgtt gactatgcaa agtctaagaa ggtatcattg 60840 gctcaagctt tgaagtacat gatttatgcc aactcggtag gtatgaaaac catgtactac 60900 cttaacagtc gcattggtgt aggtaaatct gcactgcaag atgcttattg cgagggttgt 60960 ggtgtttagt tttaacaact atgagggtcg taaaagaccc tctaaaaata actttggaga 61020 gaccatgaaa gatttaatag aaaaacatga gcgacctata tacttgctcc ataagcctcg 61080 gaaaacgatg tactatgtga gtagcacaga catgatgata aaacaaaatg atgactcatg 61140 ggtcgatggc gtctcttaca tctttacagc agatggtaaa atctacgcaa gaccttatga 61200 gatgtttaac aaagaaaact gggaagtttt agacagaaaa caagccctag aaatgataaa 61260 gaaaggagaa atcacgctat gattaaccag cacccaatct ttttaggtgg tgagagaaaa 61320 acttttgact cacttaataa acactaccca aaaatctttg aactttataa acaacaaaaa 61380 gcacaagact ggtcagaaga tgagttccct tttgaacaat cacgtcttga ttttgagagt 61440 gtcccagcat caatgtcagg tgtaatgctt gagattctta agtggcagtg ggaagcagat 61500 acccaagttg ctaagagctt ggcatttgcc tttgcaccct ttatctctga tgacatctat 61560 gcaactgcaa ttatgaagca gtctgagatt gaaaacctac atgctcttac ttactcagag 61620 attgtaaggc aatgtattaa aaaccctgaa accatcttag atgagattaa ccagaatgtt 61680 gctgtgcaag accgattaaa aactgtgaat cgtgttcttg aagaattact ggatgaaggt 61740 ataaactacc gtctgagtta tgtgcgtgat tcacttctgg acaaagaccc tttacacttc 61800 cataaagtga ttctgaaagg tctatttgca gtgactgcac tggaaggtat atcttttatg 61860 gcatcctttg catgtacttt tgcacttgat gctcaagata aatttcaggg tattgctcaa 61920 gctgtccaga aaattatgct tgacgaaatc cttcacacta aaattgatat tgaagtttta 61980 aaagaaactt taagagatga tgagtggcag aaagcttttc aacaaattct tccagagatt 62040 aaagtaatct tagatgaagt agttgaaagt gaagagaaat ggtcgtatta tatcttctcc 62100 gaaggacgtg ctgtagttgg cctaaataca aagcttcttc atgagtgggt ttactataat 62160 gctgccccac tgtatgatat gtttggcatt caaagagatt ttgtagctcc taaagaacca 62220 cctttgaaat atatgattaa gaagatggaa attgataaag agcagaatgc taatcaggag 62280 caacagaatg gcgcgtacct attgaatact gttgtagatg atttgaatag tggattttta 62340 gaggttcctt aatgacttat gtaatttact ccaaagctgg atgccctcag tgtgagactg 62400 caaagaattt tgcaaaagct cgtggtattg gccatgttgt gagaatgtta gggcaggatt 62460 atgaactatc agacctgatg gagattgcac agatgccagt tcgtcagatg ccattcatca 62520 tgaaaactga tggacaaaac ctaaaacctg ttgggacgct acagaatttt atggcagagg 62580 tgaataatgc ttaaacgcat ttgggaaggt ttggttgttg atgcaccagc tattgtgatt 62640 ggtatgctta ttgttaacct atttactgac tttgaacaag gttcattgtt tggagccatg 62700 ttactatggg ttatctttga aatattagag atacagttag gtatcactga aaaactaaga 62760 aaactctttg tgaagttttc taaaaagatt taaaatgaaa gggtctcttc ggagaccttt 62820 ttagcatgta aaggggcaat aatgaacaaa gtacagatta ttaaaaagag tggctcactc 62880 gaagaacctg atatcaaaaa agttttagca gcagtcacaa agtcagctaa tagggttggt 62940 tataaagagc ttccaccaga tgttactcaa gctcttgagt cagcatttat gaggattctg 63000 gtaaaatcta ctaagcagaa taatttactc gtttcagtaa atgatattca cagtattgtt 63060 gagggtgctt tggcagaagt caatcacgag atttatgagt cttactcaac atatagaaat 63120 taccgtaaag aagttgctca aaattgggat gaactttacc agaagaccaa agatacactc 63180 ttcttaggtg acagagagaa tgctaacttt gacagcagtt taatttctac aaaaggttca 63240 attattcgtg gttatctgac taaagaaatc tttaaacagt accatttaac accagaggaa 63300 cttgaagcca ttgaaaaagg gtttatctat atccatgatt taagagacct aatttttggt 63360 ggtatcaact gttgcctgtt tgacattggt aaagtcctga aaggtggctt tgaaatgtct 63420 ggcatcgaat attgtgaacc aaagtctgta ctgtcagcct tacaggttat tggtgatgta 63480 gttctttcag caactgcaca gcaatttggt ggctttactt tagcagagat tgataaggta 63540 cttgtaccgt atgctaagaa gtctctacgc tatcatgctg agaaagcagc atcttatggt 63600 attcctaaag aacattacca caattatgtc atggagcagc tacagattga attaactcaa 63660 ggcttccagt cactagagat gaaactgaat actgtaccat gtagtcgtgg ggattttgca 63720 ttcacaactt tgacattcgg gttacttgac tcagatatgt ccaatgaaga taaccgactg 63780 caatacatga ttgcaagtac tctcctagat gttcgtatga atggacaagg caagagcaag 63840 aaacctgttg tattccctaa actggtttat atctatgacc agaagagaca taatgagaat 63900 atctgtcaag gccaactgta cagcaaagct attgagtgtt gctctaaagc gatgtatcca 63960 gattttttaa gtgtatctgg tcatggtgct gtagcagagg cttttgagcg ttctggtaag 64020 gtaatttcac cgatgggtta acaagctcta tagctcatct aaaacgtgcc taaacaggga 64080 aactctacag gtgtagacaa tcctgtgcta aatgatgtac gatgtaagaa actatataac 64140 ataggagtct tacatgccaa ataacttcga acatgaattt gaatacatct aaatgccgaa 64200 cgactaaccg tgatgaatgt agcggtgtag ggtcaagcga ctcgaaatgg tacgctactt 64260 agaaataagt agaagatata gtctggactt actggcgaca gtaagcagct tgaataaagc 64320 ggggtaagct tagcgaactt acctgaacaa caagtgtaga gcatttttat caccatacca 64380 taacgaatat ggtgaagagt tttatgtagg tcgtgcaaac attggtgctg tatcattgaa 64440 cttaccaatg atttaccagt attctaaaga gaatggttta gatttctgga aagagcttga 64500 taagtaccta gagatgattc gtagtttcca taagaaacgc tatgaaatga ttgccaatat 64560 gccagcaagt tctaaccctc ttgcatttac acaaggtggt ctgtacaaag gaactaagaa 64620 gcctactgac agggttggtt gggatattgt gaagtccttc acagcttctt ttggggttac 64680 tgcgcttgat gagctatctg ttcttgctga aggtaaacga cttcatgaag ttggaagctg 64740 tagttttgca tacgatgttc tggaatacat caacatgaaa actgaagagt ttaaaaatga 64800 agatggcttc ttatatgcag tttatggcac tccagcagaa tcactttgtg gaactcagct 64860 aaaacagttc agagatatgt ttggtgttat caaaggtgtt tctgataagg aatactttac 64920 aaacagtttc catatgaatg ttgcagcaga tattacacca tttgagaagc aggacttaga 64980 agaacctttc ttccatatct gtagaggtgg tagaatccag tatgtcagag tagctaaccc 65040 agaaaactta ccagcactta aaagttgtat tacaagaggg atgttgaaag gtttctatca 65100 gggacttaac tttgacctag caatctgtga acattgtggt aacagaccaa aggctgatgt 65160 tgaagaatgt gaggtttgcc attcacacga tatctctgtg attaacaggg tgtgcgggta 65220 cttgtcattc acaaaaatta aaggtcagtc tcgaatgaat gacgctaaga tggcagaaat 65280 taaggatagg gtttctatgt aaaacttgac atggtggtgt gagtcttgat aggcttgcat 65340 caccttttta tttggagtta cattatggac ttgtggcttt atgtagcagt ttgtgggatt 65400 gtatgtctga taatcttatt tattcttgga gtaatgcgat ttagagacat catacagtca 65460 gattttttgc ctgagtttgc catgatagtt ggggttatta ccctttgtgt cttggcagtg 65520 ttctattcat acaaaactgt gatatggatg gaggcaaaca attgtgtaaa aactcatcag 65580 catcgtgagg ttactcattt tgtgagtgta cttagtggta gcaccactat acttgtccca 65640 caaacttctg tagagtatct ttacacatgt gataatggta aaactatatg gtggaattaa 65700 caatgacaac actcttaaaa gtattgatgc ccatactaca tatagcagtc ttcttctttg 65760 gtattagtgt tatggtatgg ggcttttcag acccacaatg gagtttttca tatcatggtc 65820 agatggactt atggtcatgc ttcaaaccat ttttagggtt agctatagca ttcagtgcat 65880 taccaactaa ggtgaaatta tgattaagtt gaaccaaaaa cagctagagt ggattaaaga 65940 ctacgcctca gagtgtggct cttgtgaaaa gaaccaccta agatactcaa cattccatac 66000 gaccttcaca ttgtatattg gtgacaatgt tctaagtgac tccgtagaag ataatgtagc 66060 attacctaat gagttgcttg acaaattagc tgtagtcact ggaacttggt ctgaagaaga 66120 cggtcatgaa ttatctaacg atgttgtctt ctacagtctt gaaaacatta tgaatccaga 66180 gtacattatg ttaatgactt gtgcacaaga ctgtgtgcca ttacaaaact tcattaaaga 66240 acactgtgaa gagtttatta ctaaacaggt tccttgtcag gtggtgtttg aatgagtaaa 66300 aggcttaaat aatattggtg aggtaactag atgaattaca tggagattag accatttgac 66360 acagctaatg gtgaaggggt tcgtgtaagc ctcttcgtag ctggctgcaa acatcactgt 66420 gaaggctgct ttaacaggga gtcttggaaa tttaatgctg gtaaagagtt tacttatgca 66480 aacctctacg gcatcattaa gttaatggat gataatgcta tcagtggact gtcaatactt 66540 ggtggggagc ctctggatga cagaaacatt caagaggtca ctaatatatg caagcatatt 66600 aaaaccgttt atccagagaa gtctatctgg ctctggacag gttttcagtt acacgaaaaa 66660 attcacctag atgtgatgaa atacgttgac gtggtgattg atggtaagta tgattcatct 66720 aaaccaacag ttaagccgta tcgtggttct gataaccaaa acctctggag aaaagagtat 66780 gggtggcaag gtgactgtca gtggagagcc gagtaagtct cgtcgtaaag tgggttacag 66840 aaaaggtgag ataatggttc tttcttttaa cagtgcttta ttgggtactt ataaaattaa 66900 gaccgagcta cttgatgaat ggagaaagta tacaaactcc cttttcggta atgttggaat 66960 ggaagcctta gcagagattg agaaggctgg aacatttgaa gttgttggta aaggtaacta 67020 cacaatgttc tacaaaacaa atttaaacgt tggtgacagt attgttggtg tcactgaaga 67080 agaacttaaa acattttgtg aaaagattga gagagatgcg aaatgggact ctggaacagt 67140 tttaaatcaa aagtaaaagc agcactagca tcattaggca tactcactgg cattgatgtg 67200 actcatctta tgcagaactt taagatggat gagaagctgg ctaatgagat tatgaaaagt 67260 atctatgtag gccgtggcaa aggtggtaag aaacaagccc accgtccgtc tggtgcagca 67320 gctattaaac gagcagctaa gaaagctcgt aaccgtaaac gtaataagaa ggctaaataa 67380 tcatgagcaa agtttataac actcgtaaat tacagatttt cgtactgtgc cagtttatgg 67440 ctaaagaata taacttctac tattgtggta caggttttat tagcgatgat gatggctcat 67500 acctcccatt taaagaagct gtgaaactct ttaatgaaga gaaaagctct aagaaagata 67560 ttgaaaaggt taagctaacc tacagcaaga aagataagaa gattatctgc ctagataact 67620 ttgtgaaagt tactggagaa gcaaaagagt ttatggatga gagtgaaatc tcattcacaa 67680 gcatcttgaa agtagctcag taaacagata ctataagggg actgtaaagg ttcccttttt 67740 attttggaga taatatgtat ctgtcaaatc tgaaacgttc tgttgcaatg tcagtgttaa 67800 gactcagttt tgaggaacgt caagagttca ttgactccca ccaatacgac ccttccaact 67860 ccaatcacat gattctctgg aatcgtgaga gtactcgtga aagagcatta tttcgatatt 67920 atccacacta caccatagac aacctgtatg aatgttttgt tgtgaagaac accattaaag 67980 tactcaataa actttgcaga tacacaggca gtcaatcctt cacattaggc caccacaaac 68040 ctgttacaaa aggtggtgaa catcactgtg caaactggtt tattcagact aaagctgata 68100 accagaagca aggagatagc ctcctaagta tccctaagat gacctatgaa gagcaagaga 68160 gatatatcaa aaataatatg ccagatgtgc ttgacaacaa ctatacagat ttggcaatat 68220 ctctcctgtt gaagttcgag atagtttata gggcaactta caatggctaa agaaaagtgg 68280 gagattttac cattagtcag tgaaggcggt aatggttgtg aaatgtacat gatacgtggt 68340 catgttccag agccaattgc acttgagatg gtaaataatt atacagatgg ttcctgcaaa 68400 gacttaggag aaccaactgt caagcagcaa tgggtaaagc ctgtgccaga tagtacagga 68460 aactgtagtg tactttatca cgttgtagac cctgtaaaat gcaagtctgc aatggcagta 68520 actaacgtaa cttttgattg agagagaaaa ttatgaaaac atctatacgt gttacagttc 68580 attcaccaac taaaggaact catgaagaag agtttaacat catccaattt ccttctggtg 68640 agattggtgg acacttttca ccagagtttg ttgattttac tgcttatgca gcatcatcta 68700 tcaacaatgt gattatgatt gtaaaaggtt atgataagga cacattgttt gctgtggcac 68760 ttgctaaaga agcaattgat gatttagtac ctcataagtt tgctatgaag actgctatct 68820 tttactattt gccaaatgca cgttacgacc gtcacatgtt taaaggtgat gcagcagctt 68880 tgaaagtgtt tgcacaacag gttaatgcaa tgggttttga tgcagtctgt gcagttgacc 68940 ctcacagcta tgtaccagat aacctgttta actgcttcca gagtattcct caaaaggaaa 69000 ttgcagtcca ctatgcaaat gacccacgca ttgatttctt ggtagcccca gatgcaggtg 69060 cagctaagaa gactgaagag actgctaaag aggtggataa accatacatc acaatgtcta 69120 aagtacgtaa ccttaagact ggtgaaatta ctggtatgcg aatccttgat gatgttgatt 69180 tgaccgataa aactgtcatg attctcgatg atatctgtga tggtggtcga accttcatag 69240 aagcagctaa acatcttcgt gaagcaggtg caaaacgtgt ggaactctat gtaactcatg 69300 gcattttctc taaaggtgtt gaaaacttac ttgacaatgg tattgaccat atctacacta 69360 caaactcttt aggggaagct aaagaccgtg gcttaacaca ttatgcccaa gttactgtaa 69420 ttaccattga ttgaaacttt atagggggtt tcaaagcccc attttgagag agattaaaaa 69480 gatgactaaa tcactttatg cagtaccagc aggtttaaac gcggatgctt acaaatctgg 69540 ccatatttat cagtatccta gtgcaacaga gtacctaatg ttcaacctga caccacgtag 69600 tgacaaatgg tttaacagcc ctttagcaat tgacggtgta gtggcttttg gtattcaacg 69660 ttttgttaaa gattacctga tagaccactg gaacgccacc ttctttgaac gtgacaaaaa 69720 agaagcaatt gacgaaatct tagaagtcat gaacggtgtt ctgggtaaag atgctattgg 69780 tcgagaacat tgggaagcac tccatgactt aggttatcta ccagttgaag tatacgctgt 69840 agaagaaggc acagttgttc ctatgcgtgt accaatgatt gtcttccaga acactgtctc 69900 aggtttccat tgggtagcgg ggtatctgga agatgctttc tctgctgaga tttggaaggc 69960 ttgtaccatt gcaactattg cattgcacta caaacgtatc tgtaagaagt ggtctgacct 70020 tacttgtgac aacgacttgc atttgcctta tcagtgccat gactttgcta tgcgtggtat 70080 gtccggcttt accgatgacg catttaacgc tgtaggccac ttaaccagct ttaaaggaac 70140 tgatagcttc cctgctgtgt atacagctaa acgcatctat ggacagtcct acccaatctc 70200 tgatattggt agctctgtac cagccactga acactctgta atgtgtgcaa acattgcttg 70260 ggaaggtggt agtgagttga ttgaagaaga aagacgcttt aaaggtgagt tacaaacctt 70320 ccgtcgtttc ttaacagaaa cttacccaac tggtattgca agtgttgttt cagacactta 70380 taacttctgg agaactgtat cagtcatatt accagaactc cgtaaagaga ttatggaacg 70440 taatggcaaa ctagtaattc gtcctgattc tggagaccct gtacatattg tcacaggata 70500 caaagcaatc catttagagt gtgctaaaaa ggcttattac gaacacctaa gtaagctgga 70560 agccagcgac acaatgctga atgctgttct gaacatgaag cttgaaaaca tcaattatgg 70620 tattgctgga tggctactgt cagaaggtta cgaaatggtt gttgacagag aagactttga 70680 agttgctgat acagtgctgt tgaaaaatgc ttatatggtt ggttctgcaa acgttgtaac 70740 acgtcctgta gcggagattg atggagctat caagacactg tataacatct ttggaggaac 70800 tatcaactct aaaggtttta aagtactgga tgagcatatc gggcttattt atggtgactc 70860 tatcacgttg gaacgtgcaa acgaaatact gaagcgtttg tatgaaatgg gttttgcaag 70920 ctctaacgta gtatttggtg taggttctta cacttaccag tacatgactc gtgacacttt 70980 tgcatttgct gtcaaagcaa ctcttgcaag tattggtggt aaagagatta tgcttgcaaa 71040 agaccctaaa acagatagtg gtgttaagaa gtctgctttt ggtggtgtag cacctatgtg 71100 ggatggtgaa aatcttaaag ctgtagatgg ctatggattc cagagctttg cagatgcact 71160 tgaacaccca gcttgtgctt tacgtctagt ctttagcgac tctgagcagt tcggttacac 71220 aactcttggt gatattcgaa ataatattga caagcagctt taaaagtata tgataagagg 71280 ctcctacggg agccttttta atttctggag aagattatga aaatcaaaga gatgaacatc 71340 aacattgtct tagaagagcg ttgggagaac atcaagaagc ctgaaaatgg tcataagttc 71400 ctcaataaaa tcttagtagc agctaaagaa gaactgactg gcaagattgc agcagcaatc 71460 acaataaagg tctgtgtaaa aggtcttcca gacaatcacc aatttgcact tgacgagttt 71520 aaagagagct tctacaatcc aaacaaacag atgcttgaaa gtaactttgc agtgtctaca 71580 agtatcgtcc atgacagaag ctttatcctt tacaagaata tgagaggtga gtcatgcaag 71640 catattggat agaaattttg ctatcacttg gtagtgtagc agtgtttgtt tatcttcttt 71700 gtaagtactt tgcagagtac aagaagtgtg attactgcaa cggagaaggg tatacaagag 71760 caggttgttg ccctatgtgt ggcggttctg gtaaaatgtt taataagtaa tttaacagta 71820 aactaagagg aaagtattat gcgcatggta aatgaccacg cagaagtgat taagagttca 71880 acttctttag agacgtctca agcacagatt acaatgacac ctgagatgtt cagccttttg 71940 agttctggtg tatatacctt taaagaaagg gcagtgattc gtgaactgtc atgtaacgca 72000 gtagatgctc agaaagaagc tggaaaagag aacatcccgt tccatgtgca tttacctact 72060 cgttttgagc cttactttga agttcgtgat tttggaactg gattgactca tgataaagtt 72120 atgagtttgt acctaaacta cggggcttct acgaagaatg actctaatga ctacattggt 72180 gcaatgggta tcggctcaaa atccccattt gcaattgctc agtcattcac agtatccagc 72240 tatgttgacg gtgttgttaa caagtactct gtttaccttg agaatggtat ccctcaagta 72300 actaagctaa ccaccaaccc aacgaatgaa cctaatggtt tagctgtacg tgtagcagtt 72360 gctgaccatc gtatttcaaa gttctttgaa gaggctggta atgtatactc atactttgct 72420 gtaaaaccag aaagtaatat tgagtatgac gatgtattag cagatatgaa cgtcattgcc 72480 cgtgaagaag gtgtctatga tgccatgatt cataagcaaa gctggcgttc tagtggtaac 72540 aggacagagt ttaatgtggt gatgggtaac attgcctacc ctgttaatat ggaagcatta 72600 cttggtgatg atttcttcaa agtattgcca gaatttttcc gtaggagcgt agaccttgta 72660 aacatctaca tgcctattgg ttcagttgct attgcagctt ctcgtgaagc attgcagatg 72720 aatgacacga caaaaaatgt tatcattgag gctactaaaa agataactga agcaattaca 72780 aaggatgtta tcaagaaagt taatagccaa cctacactca tggatgctgc acaggcttat 72840 gctgagttac gtttgaactc acgagagatg tttaatgctg tgtgtccaaa gctagagtgg 72900 ggaggcgtta agcttgattc tctggaagaa gaattactaa acattcgtcg tggaattatc 72960 tacgcagaag acggctcagt tgtctatgaa cgcgatggta aaggtaatat taaggttgat 73020 aggaacggaa acaacatccc taaagtagat tatctttata atccagttgc ttatgttaag 73080 tttaactctt tagagagtaa gattcgtgca acagcacttt cctacactca agaggcgagt 73140 atgttcaata tctttggtgc aatgcgcaaa agccaaattg aacagttttt gtttgttatc 73200 aatgaccgtc gcaataaaaa tggtactgag aagactgtag gacgcaatca gattctgcgt 73260 ggtgcatgtc gagactatgc cagtgagtca agtctgtttc acagatacaa tggtattgtc 73320 tttgtattct cgactgaaaa agagttagac gacctaatca atctgcacaa acttgataaa 73380 agcttgttaa agattgtgaa gatgtctgat aaggaacacc attatcagcg taaagaagct 73440 gtaagaggtg ttgtaaaact ttggaaagct gttccagcag aaggtgtagc ttcctataaa 73500 gaggtttcag aggactttga tacaattgaa gaacctcagt tttatattaa ggcagtaggc 73560 gacacagttg atggcgaatg tttttgctca tctccagaag atgtagctaa gtcagttgcc 73620 aatgtcattg gaaagacagt atatgttttc cgaaaagcaa attggaaaaa gataccagaa 73680 gactggattg aagtagatga gaagctcttg aacgacagct taactgatgt tcattggatt 73740 aatcataaca gatatatgac acgtatctat atgaatggtg tcctcgacct tacaagcagt 73800 tggattattg ccagaaactt tacgttcaat aacagaaaga tttctcgtgg ttattgttat 73860 tcacgagata caaacaaaac tatcttctta gaaggtaatg aagatgctgt tgaggcaatg 73920 tttggtaaaa tccaatacgt tgctgcaccg tttgcataca cttacactat tagtaggttg 73980 caaactttga aagattgcct tgacaatgat acaaagctgt acaagaaaat taagaaagct 74040 ggtgaccgta tggtcatcaa ggtgacaaac tatctttcaa aaagaaaaca agaaaacttc 74100 ttgctttctc atttagattg gaataaagtg tcacctattg aagtgagtaa gttcttaggc 74160 tttgatgtga agtgtgttcc agaaggaact acaatttacg attaaagtgt ttgacaaggg 74220 gcttcaaagc cccttagaat agccttacaa aatgatttat taactaacaa gagagagtaa 74280 taagatgact actaaaacta aagcacagat tgacgcagaa atttacaaac tggttaaaga 74340 aggcaagctg actaaaacag ctattgcaca gaagttcaat acttcaaccc gttcagttgg 74400 tcgtgctgta gagcgtcatg aagcaacctt aaagggtaag aaaaagacag cttcgacacc 74460 agctacgaag actttgaagc aagttgctaa gacctttaag aagaaagctg gcaaaccagt 74520 tgaaaaggtc gttaaagaca ctgtacagaa agttccagta aataaactgc acgaagctat 74580 gcagaaagat gacaagattg agtacatgat tactggcgac tctgtaatta tgacttacgg 74640 ttcagaatct gaaattgttg agtctactca tccgaactat caagagattg tagtccatgt 74700 tgtgaaaggt gagttcaaga aagctttcga actgatgaac attcgtaaat ctatcgaaaa 74760 cttcactcag ggtgctatca caatcaaagg tgacaagcta ttctacggtg ctgttgaaat 74820 gcgttctact ctggttgacc gtatccttca catgatgaag actggtgata aaggttttga 74880 acgacttgta atgttcttcg aaaaactgat ggaaaaccca tctaaagact ctgtagaaca 74940 actttgggga ttcgtatctc accttgatgt tgaaattgat gaagaaggct acatcattgg 75000 ttggaagaaa gtctctactc gtgaaggcaa gctggttgac tctcacacct acaaagtacc 75060 taatgattta ggtaacattg tagaaatgcc acgttggatg gttgataaca accgtaacgt 75120 aacttgctct cagggtcttc atgttggtgc ttgggattat gttcgttgct tctcaggtga 75180 tacaatcctg aaggttcgtg tccatccacg agatgttgta tctgttccga ctgattacaa 75240 cgatatgaag atgcgttcat cccgttatga agttgcagca atcgttgata atcaacgtaa 75300 agtgttgaaa gcatgggacg gtaagactga agctttgcat gtcatcgtcg gtactgctgg 75360 tgaactcatc tcccaacgta aacgtgaaat ctaataagta atttcttaaa aggctgcttc 75420 ggcggccttt tttatttgta ttttgtgcag agtgctgtat aattgttgtc acgataaact 75480 aaacaggaga accaatgaag aaattgattc taggattgtg cttaatgttt acagcacatt 75540 tatcttatgc agttgactgc ccagagctat caattagcca aaaagttaac atgttaaaag 75600 cttaccagta tggtgagaac aacatgggga aaggctgggg tattactcta gcagctatag 75660 ccttacaaga gtcagagtta ggtctgaagg tagagaataa aaagacccat gactatggta 75720 ttttccagaa tcacttgaag actgttgtaa agcgtaacaa aattagtcct aatgtggcta 75780 aaaagaaact cttaaaagat tttgactatg cagcaaaaga gacacataaa gagcttgagt 75840 tttggactaa ggtacatggt cacccgaagt caaagaagac tttacaaaaa gttttagcat 75900 catataatgc tgggtattcg tacaaaatcc ctaaagctaa gaaatactct caagatgtct 75960 ataacaatat gaaagttatt gctcaatgtg aatttgcaac aaacatttct aaggtaaatc 76020 atgaaaaaat taagaaagcc tgatgaagtc ctgtgccacg cttatgactt gcatcctcat 76080 gagttaggtc ttgattcctg tgtatggact ccagaacagt gcagggattt tgaagacact 76140 gcaagagaag ttgtatgttc acttgaagag ttccacacat cagaaccaat tgtgaacgtt 76200 gtggataaag aaactggaca agctataggt gtaagtcgtg atagtttagt catagtcaat 76260 aaagaccttg tagaaaaagg taacctcatc ttagcagata ttgatggtgt tcttacaaat 76320 tttaatcacg aagattgctc aacggaattg actgatgggt cattctcaca gtacactaat 76380 cttcttgact ctgtaagagc aaaaccaaca tatgttttta gtattattga tgcaattgct 76440 aatcatgcta ccattggact cttgacagct agaggtgaat ctcagagaat acctactgag 76500 atgtttttaa ggcataacat agagcatgat tacatgcttt ttatgcgtgg ttttggaact 76560 aactctataa gtgcagaaag tttaaaagct aggatgattc agtcttgcat tcttccttac 76620 tttaatatag tatgctttat agaagataca gagaagaatg ttcagaaggt aaacagaatc 76680 cttccacaca ttaaaaccat gttagctaaa cattgagaga aaaacttatg aacaatatca 76740 ttacggtagc actggacgat acgacaaaca aatctgtagt agttcgtaaa ttgattcaag 76800 gtaactttga aggtaaaatt tttcgtgctg tcaatgtaaa agctgatggc agcattcgtg 76860 aatatcgtgc attgttgaac gttaagaagc acgtaaaagg tgttggttca acgactgcac 76920 acaaagaaaa cctgatgact atctatgata tgggtaaggc ttcagagtta ggtgctgaag 76980 gtatcgttaa agaaggtgct ccgtatcgct ctttcaatct ggaaactact cttatgcttt 77040 cctttacgag tggttctaaa acaatcactt atctctttac tgatgctgca acggtatctg 77100 ctattaagga tggcactgtc aaagctggcg tagttgctgc tgcaaaagct tcttcgatgg 77160 ctgcaaatat ccttgctaaa gtcctcggtt aaggttaaga tacaggctcc ttcgggagcc 77220 tttttcattt caggagattg ttaaatgaca tttaaagagt tctgtcaagc tactttcata 77280 attgttttct tagttggggc aggtgtctgg ggaggatact cttacagaga ctaccaagtt 77340 gctgaagctg agctaaataa tgagaaacta ataagtgttg ctaaagatgc ttatcaggaa 77400 ggtttagcca cactcagcac caattacaaa aatgatttga aagacgtgct tgctaagaat 77460 aagcatacaa aagaggtact aacgtatgaa aagactaagc cagagtttta taatgtttgt 77520 gttactgata attatgtcag ggtgttcaac gaacaaagtg aacagtacat tcaaaaactc 77580 ccaagtaagt gagagtgaca agtacactca agaagattca agattcactg tcaaaggtaa 77640 tactggtagt gatgtggcat cagcccttga gttttaccgt gatggttttt accagtgcac 77700 aatcaaagct aataacctta ttgatatgat tttattagga aacaaacagc aatgacagag 77760 aatgaagaca ctttctatgt agaagggtac ttactgctac cacgaccaaa agaaacttat 77820 atgcgggttg atttctcacc aaccattatg gataacgtga tgtgtcatat ctttatgcaa 77880 ggtgtcacag cacaacttaa gcatgttggt aaagagtgca aaataaaggt tgacactcat 77940 ccagaaatca aagagaatca ctacacatgg ttcttaccag actctaaaga aatcttagca 78000 gttcttaaaa cgaggaaatg attatgcaat tccagccaac aaatcgtggg ttttcttatg 78060 cagaatttaa agatgctaaa ggagagattt gtactttaca agaaagttca tcatatgaac 78120 catgtatctg gttaggtgct aaagaggtct ctgttcagta cctagatggt ggctggaaaa 78180 cacttgatat cccagcatta gtaggcaaag attttgtagg taaccaaaga atgaaactca 78240 accaagaaca ggtgagagag ttattaccat accttcagta ctttgctgaa aatggtgaat 78300 taccagcaga ggaacttaca aatgcagatt aatggaagag attttgtagc cgtttactac 78360 gagaacgata aagaagttgg cgtagcacaa gtatcttatg gcaatggtaa gtggttgtat 78420 gggacaatcg cagtagtcgg aacaagaagt gatacaaaaa cttttaaaga ctgtgttgac 78480 cttcttgaag aatccatcca caatcattgg tgtctgatat ggatgactga caacgaagtg 78540 atagaacgct tcaaaaagat tggtattaac attgatagca ttgagcatgt tgatttatac 78600 gagctaactg agaaggtaaa ctatgaaagt aatactagcc agagataaaa agacccgtaa 78660 gattgtacgt tcagcagtat tcatagggag agatgaagtc gtcccgtttg caggggaaga 78720 tgtgttaact tataagaagg gcttaaaacg taaattcagt gaccgtttac gtggcttcat 78780 ctctgaacca ttagaagatt tcaaaagtcg ctgtggcgaa ggcatcatca ttcaagaggc 78840 aattattcat ggctgatttc tgcaaagatt gtgctatcga aatgttcgga cgtgatacag 78900 gtgacttaaa aggtctcatc actgaagatg actttaaagc tggttatgca atgccagtaa 78960 tctgtgaagg ttgtggatgt atctgggtag accatgaagg acaacgtgta aaaccttcag 79020 aagataaaga atcttgggag agatgttaaa tgggtattgt aaaaatcatc aatggggata 79080 tcttctcagc atttgataaa ggtaagtttg atattattgg tcacggttgc aactgtatga 79140 atttaatggg tgcaggtatc gctgacaaga tttctaaact ttatccaaaa gcatatgaga 79200 ccgatacaga agtttatctg tatgcaggtg gcataggcca caaaccttgt gaaaatttac 79260 tagggaattt ctctgtagca cgtttaaaac aaggccgtat agctaacctt tacactcagc 79320 ttaagactgg taaagatgcc cgatacagtg ctttagagtc atctttgaaa caacttaaca 79380 gatactgtga agtcaatcag ttgaagaaag ttgggttacc tatgattggt gcaggtatcg 79440 gtggactaga ccctcaagcc gtcgcagtta tcattaatca ggtgatgaag agtgtagatg 79500 tgtatctgta tgtctacgaa ggcgagatgt accacaagtt acgctcaggt tggaagaact 79560 actgtgaacc agaatacttt gcaggtgtag taatgttcac agatgataca gttaccctct 79620 tcagacgaag aaaaggtaag atacatcaaa gtaaccctcc ggttgagaag atgtctctaa 79680 gtaacgctct agttacccac ctgtcgaaga gcaatcacag aattgcagta acatttggca 79740 gtgatgcaga tacatatatc tatgcaagaa ctgatgagga tgttgaggaa attttctctt 79800 caccggaagt taccttctta gacgcaaaga actaagaaac tctgtagaat attcacaata 79860 gactaagcct ttcttatctg gtaaattttt caggtaagga gggctttttc atttcaggat 79920 tgtgcaggta gagttctgta gagaaatctg tagagaaaat tttgaactcc aaagagaaaa 79980 ttcattttca aactgtgcag gtaatctcca aagagctttt tcattttcaa aacgtgcaga 80040 ccccgctcag gtcgggcaag aaaaatcatt ttagaattgt gcaggtaggt gggtgcaggt 80100 ggggatgtcg ccatttctca caaggctacc acatctggaa ataaaagtca acagaaaaat 80160 ttgtagcgga ttgaaaaata attcttgatt gtagttctgt tttgtggtag tcgcgtgcgc 80220 ccgtttcatt acagtttttt gcaaaaatat ttttaaaatt tttcttgact ctctgactat 80280 ggttatttaa tatttgcatc aacgggggcg atgacctccc agctaactaa ccttaaacta 80340 ggagattcaa aaatggctta tcgtgcacct aaattcatca acaaagacaa ctttcgcaac 80400 gtactggaga aggtacttga tgaaaaattt aacggtaaaa taattgttgt tcatgcgttc 80460 aatttcaagt atgatatcaa cgggaataaa atcaaccatt acacggcaac catgttagat 80520 gatacgctat caagtgaaaa agccgtactg aatgcactgg caggacgcgg aaaggtatta 80580 gtaagatgcg ataagagacg ttatcaaggc ggcgcatatg gttacgatga tgctgtttat 80640 catcttgaaa atatgggcta ttcagtggag aaagctggag cgtctcaaat aatcggtagt 80700 gatggttatg tgacaatttt caaaattaac tgataaagta ctttacaagg ggcttatagt 80760 tcgataaagt aagccccaga taaagagctt taccactaaa tcctaaattg gagattcaaa 80820 aatggctact atcaaaaacg ttgtaattcc tgtaaaaact cgtgatgacg ctcgcatcat 80880 ggcgaaaaag ttaggcggga aagtggttga caacggaaaa caatctgctg taagatgggg 80940 tgtgaaggct gataaacagt tgaagttaaa aaattcacca ataaatcttt ttacatgtgt 81000 aaacactatc ggaaagacaa atgtatacac gaaaaaggca tatattagac gcgtagcctt 81060 aacatctcca attcgtacaa tgagaggtta tgcaaagctg aaaattagct gataaagtac 81120 tttacaaggg gcttaaaagt tggtatctta agccccagat aaagagcttt accactcaat 81180 cctaaactgg agattcaaaa atgaaattat caaataaagt tcgtgaaaat ctaagcaaat 81240 tactcaacaa aattgatttc attgaaacat caagaaatag ccatgatgtt atggaatggt 81300 tacacggcaa aataaaaggt cgtcgtgtag tgatttgctt atggaatagt tgcagggagt 81360 ataaaaaacc tgtattgcaa gttaatatct atgatgatac tcttaaaagt cctgtcaaat 81420 ctaaaagcga tttactagat tcttatgaga taactgtaaa tggcaaagta tcacgtaaag 81480 aggaagcacc atgttaatga ctggtatcac tgcaatcatt gccattttcg cgctctataa 81540 ggcatataaa gcctataatc tagcaaataa ggcaatcaca cagcaggtta gcaataacct 81600 tgtaatgagc tttctagagc ggttgagcga tgaacaatta aagcggttag aaatgagctt 81660 cagattcagg gcgagaactt atcaaatcag cgacattttc aaagatgatt ttcaattagt 81720 cgatgattat aattctctga taaatgcttt aaatattagt gacttaaaag actatcatac 81780 tgtaattgtt caagagttga gcaaacgaaa acaaaactgt tgacactgat ttttagatag 81840 attaaattac acatcaacgg ggaggcaata aacaccccac taaataaagt ccttaaacta 81900 ggagattcta aaatgtatac tactaacaac ggtcgcactt taaatgtcac tctacgtcac 81960 tatgtagacg gtgtgatgca ttttgaggat ttacaggctg agcaacatat cttagattgg 82020 caagtcgcag gactccaaaa aactgctacc ggatacggca aaaagattcc aactagctgg 82080 aaagtatact atgaaggacg tttgcgcaga atttatcagg atgtatgtag caatagcaca 82140 tcaagttaca tcatagtaaa aggtaaaaaa ctgcatttag tataaagtac tttacagggg 82200 gcttattagt ctgtacagta agccccagat aaagagcttt accactcaat cctaaattgg 82260 agattctaaa atggctatta ataatcgtga gttgtcaatc ttaaaagcac gtttaaccgt 82320 taaccgaatc aatgttatta catcatcagc accggatgaa acattgcaca acattatcgg 82380 aaagattcaa agcgttatct tagacgttga aaacgtaaaa aactcattgg ctgacgttgc 82440 agcaggtatc acgctagacg gtgcacaata tgaaatggtt gacatgttag gcaaatctaa 82500 ggtaatgaat aaagagttag acttgaaaat gtttagattt gccgtgaaag tgtggctatc 82560 tgttgagtat gatgctaatt ttgcaatcgc tgatttcttt gccacttggt tacaacgtaa 82620 tttggcaaat catgactttc gtgacatctg cgacgcaatt tatgcagaac tctaaaaatt 82680 ttattgacac tgatttttag atagattaaa ttacacatca acggggaggg aatgaccttc 82740 cccactaagt aaagtcctta aactaggaga ttcaaaatgg ctattatcaa cggtttaacc 82800 attgaaacca cgcacatcaa agacattaaa gttggtgatg tagttctttc ccacggcgta 82860 gaaaaaacag tcactgcaaa ggatatcaaa gatgattctt tcatgggtag aacactcttc 82920 ggtgattctt attgcttagg ttatcttgca gttttaaaag ttgtcaaaaa ttaactgata 82980 aagtacttta caaggggctt atagttcgat aaagtaagcc ccagataaag agctttacca 83040 ctcaatccta aactggagat tctaaaatgg cttatgtaac cgtaattacc gataaagctg 83100 gctcatcttg gtcaactcaa gtaagtgata agatgtcacc tatgcagtgc ctaaaatact 83160 ttgaacagtg gaacaaaggc gagggtgtaa gcccctttca agtgatgcaa atcatccata 83220 cagataacga agggaacaaa acgaccttga acagtgaata ctacgcaagc cgttttgaaa 83280 cgagaagtaa aacaatgaag cttttacgtg aatccggcta ttctcatatt gccgcgctaa 83340 tctgggatga cctgctaaaa agtcagcgta tcagctatgt aaagcctgaa aaaatcttca 83400 taagttaatc aataacttac aaaaaacttt taaaaaagtg ttgacaatac ccattgacat 83460 tggtaacttt gttcgagggg ggttatctaa aagggattca cttaatagat tctttataga 83520 taaacttaaa aaattgcttg caaaggcaaa tcaaaacaag taaattacta atcaacggga 83580 agggattaac cttcccacta agtaaagtcc ttaaactagg agattcaaaa tgaacaagtt 83640 tcaagctatc aactatattc gctcatccaa cgttatgact aagcccgtta aagatacctt 83700 cgaatttcgt tgcaacggtg tacactttgc aactatcact aaatcagaaa acggggctta 83760 ttatatacat cgtcgtaatg cctctactgt tgtagtctct cactttatgc aagctgtagc 83820 agaactgtta ccgctattct taggtattta cttagatgag tgcaaaaacg ttcgcaacca 83880 cgttaaagac cttttaaatg gttataaaat ggcttatgaa cgttctatca agaaccttaa 83940 caagttctat aaagcccctg aaaatactgt ttcaatggct tacagtgtaa gcggtgagtt 84000 ggttagcctc tttgaagtgg aagaaatcgc gttgcatgac ttacgcccta gcggttactc 84060 taagacacta gaggaaggtt taacgttcaa tatcaagaac ttagaaaaaa ctttaaaaga 84120 tattgaaaaa gatattgacg agacaatcaa aaatctgtaa attgtcaatc aacgggggat 84180 aacctccccc actaaacaaa atcctaaact ggagttatca agatggaaaa cgtagaatta 84240 accacacttt tacaaattgc aaccgacaaa gaagatgtag cattattcca ccgtatcgaa 84300 agcgatttgt tcaatggtga aatcattaaa gaatcttacg aagttgtcgg attcaaagat 84360 ggtaaagcat ttggcttttt tgacctcgac aaaagcaaag cggttacaat gtacgatgtt 84420 ctatcttgtg aaattctgac aaaatacaac gaaaaaaggc ttgcaaccta cgccaatatg 84480 cagtaaatta ctaatcaacg gggagggaat gaccttccca ctaaacaaag tcctaaactg 84540 gagaatctaa attatgtcaa ctatccgcgt aactgttaat gcttatggct gccacaaact 84600 tgatgcaaaa ttcgatatcg gtggaacaga taacaccttt gaccttatca aaatgctgat 84660 tcagcaggtg ataccagatt ataaccctac tgatgacttc tggaaagatg ccgtaaaaga 84720 agccataaac gaggaatctc actaccatta ttggggtgac ttgattgtct ctctgtacta 84780 cagcaaacaa ggttacaact aatcgacgca gcggggctaa atagccccag atacctgaaa 84840 agctccttct cataggggct tttttacgtc tataaagaga ctctatacaa tccctttcct 84900 ttccttccta gaatctatcc cgtaccttaa aagcaataac tctatagctt actctatagc 84960 cccttatcaa atcaacttaa atataagccc ataacccgaa agggaagggc aaccatttaa 85020 acgctctata aagccctata acagccttat caacctaact taagcaatca tattaaccca 85080 ctctaaaaag ccctttatag agctttctag agccttaaaa gaatcttata agggtgtgag 85140 ctttatcatc tcgttagcta tcttgtgaac tactaaaaag ggcttgttag tgtcttaaaa 85200 gtatgttata ggggctataa agttactttg aagtagctta aaagttacgt aaatgctagc 85260 85260 <110> Optipharm.CO., LTD <120> Novel shiga toxin producing Escherichia coli specific          bacteriophage ECOH7 and antibacterial composition comprising the          same <130> 1-66P <160> 1 <170> KoPatentin 3.0 <210> 1 <211> 85260 <212> DNA <213> Unknown <220> <223> Bacterinphage ECOH7 <400> 1 ccccttaaca ttttcttaac aatttcttaa caatccctac atagttatgc ttttaaggca 60 cttgttagag acttgttagc cccttgttaa atctctgtta agaccacgta aaagccttgt 120 aagagtcttt agcatagcct ttaaagcctc tgtaaagctc tataaagagg tatctataca 180 gttggggtgt aattctagga agagacttaa aagagcttac aaggagctat agagagcttt 240 taagagggct atttagagaa tcactaagag ggaaagggaa cgataaggaa gagatagtga 300 ccatgttaat agcttgttaa aaagattaaa cacttgttag aaacttatta attaccttaa 360 caaaataact cttaaaaatt aacttgttag ctcttgttag tcatctgtta agattctgta 420 aagcactatc aagccttgtt aagaaactgt tacgttcttg ttagttatgt taagaatctg 480 ttaagacctt tggagagttg ttaagaagat gtgagaactg ttaagagatt gttaaggata 540 ttttaaaatt ttgcaaaggg ggtgttaagg agctgttaag ggctaccaag cagatata 600 tcctagacag atatttttca gttccttcaa agaccctaaa aaatgacttg atagtcctat 660 tttataaaaa ttttaagatt ctgaataatt acctagacag tcttttaaaa atacaggcat 720 aaacaggctt ataagatacc ttgttagtac cgtgttagac cttgtgagat aaggtctgta 780 aagatattcc tgttaagttt gttaaaacag tctatataga ggcttagtag tgcctagtga 840 acacctatag agagtatcat agctgacata gagattaagg ggtgtataga ggcttctaag 900 agcgtataga gagatggtta aggtgattgt ataggttgtt aagatggctt gttagaagag 960 tgtttagaag gcttattaaa gggctattta gaagggttat acagaggggc tattaagacc 1020 actagatagt tactaagata ctatatagta ttatataact atatagagag taagattaag 1080 aaggttgtta agaaggatag atattttatt cacatatgat ggtgacccag aggttgggac 1140 aaccgataaa attttataaa aattaaaaag cccccaatta aggaggctct taagatattt 1200 taaatcagtg tttccagaac tgtttttagt gatgacttgg ttgtctgata aaaatgagta 1260 aagccattgt tgttatcaaa tacctgcatc atgatatcat ctcccttgat tgggtacact 1320 cggatatcat agtggaaacc attaaagaca caagagccat tacagaatgc actaaagtca 1380 tccactgaga tatactcttc atgtcctgtg atttttatac agataccttt actcggtctc 1440 ttaggattaa tatcttccat cacagacata ttctgtccac caagagatac catagtgacc 1500 actttacggt tcttctgttt aaagtacatc ttaactttct gcaaagtgtc ttcatagaac 1560 ggataagact tctctacaaa gattccttca agtggctctt taccattttc aacttctctg 1620 gtatgccgta ctgcaaagat aatttgttgg aaccagtcaa gattttcttc ggagagtgct 1680 tctcgaacat cactccagtc aagaactagg tagttgcctt caacttcttt taagttatct 1740 ttttccatat ctatcacacc attatcgtaa aacttggtaa gctggtactg caaacttgtt 1800 gctattgttc ctaataaggt cttcttctgt tactaagaat gtaatcccac ataagtgcgt 1860 atagtggctg tcatactcaa atattttaag taagtattta ccatcatgat aaacagattc 1920 tactacacct tttgcaacaa tctttccagt cttgaagtct ttaagagggt atgtattacc 1980 aactttaggg actgttttca cttcaggttc ttttagcttc tcactagctt ctacatcacc 2040 tagtaagtta gaactttcaa aagctctgaa tctcatataa gcttctgaac attcccagat 2100 aaaacaatgt acaagaacct tagcagcatg tcttgagcaa ttcaactcat cacagatttc 2160 tttgtacatt tcatctttag ttgaattggt aagcattgct tttcgaactg tttctttaat 2220 tttcataagc tctccgaata accctattaa gcatctctaa agtcagcttt ccagattcta 2280 aatgtggcac aataagtttt aaagcttttt caacatcatg cacaacatct attatacctt 2340 ctgcataggc ttctacaagg atatctttta cattttgtga tttacctttt tgtggtatca 2400 ctagacgcat atcaaactca tttgcttgag gatggttctt accttgccaa taaccatctt 2460 tgttaaagct gatgtagtca ccgtggtaaa aatgtacacc attagcttta aacttctcca 2520 gcacaaggca ttgaattggg tattcagtat tccactccct gactcctata accttcactg 2580 ttgcgatgta ctcctgtgtt tttgcatcta tgatgtcaag ttcttgacca gctatgattt 2640 gcatattcgc taccttctaa gttctacaaa ggggctatca agtaacccct caagttatta 2700 ctcagacttt tcagtcttct tgtcggattt cttactagtc ttcttctcag ctttctgctc 2760 aggctccttc tcagaagttg ccttgataac catatcaaga agctctgata agtcactctt 2820 cagtaaagta acaggctgct cgttgtagaa gtttttgtca agataaactt tgaactcttc 2880 tatagaagga aatccaaaaa ttggtacagg tgtcttaatc attacttagt ctctccatag 2940 gcatctttta agattgcctt gttaatcatt tcagcgattg caaaacgaac acatacagct 3000 ataattgtag gttgcattct ttacttacct cttcaagttt aacctcagaa tccttaagtc 3060 gtttcttatg aactctgaac cttattagtg agatgatgat aacagctaaa ggaacagcat 3120 acaagtacca aacagcatat acctttgcag taatctgtgt aatggtcaag tcttgagcta 3180 tacaactgat tgctgatact gccagtaaca ttgctaagaa gaacgccagt accataacaa 3240 tacttaggca tcctctagca tggtgttcat gcttaatgca cttattcttt tcgtcaacaa 3300 ccctgttgta gtcatttgta aagtctctgt agaaagcttt caagtattct tgagtgtagc 3360 cttcaaagca acctgtacca taccaataat caccaccaaa atgtgctatg ataccagtat 3420 ctccatttgg tttagttact ctcaaagatg tgtgatgacg agtatcttga tagtctattt 3480 tgtatctttc ttcgtaaatc atttcccaac ccctctcaat cgcttgttcc attcaaggtc 3540 aataaacctt tgcaggactt tccctctagg tgttacacct tttaaatctg catcgtaaca 3600 atggtaaaag tcaccttcag cgtgtttacc atagcatttt ctgatattct ggcttctgcg 3660 atactgttct aacaaggaca gtttgtagta atcttcaact gtagttagtt ttttcattct 3720 ttcaccgtag tgtttctcca gaaactaaaa agggaactac ctctcgatag ctcccatcat 3780 aatcaatatt gtatactcgg tcaagagtat tttttaggca gtaatgcgct acgcttgtta 3840 ctctcttcac tcaatagtgc gataaattca tcacctttcc ctttccacgg actgaatgta 3900 gggatgtgct ctctgatagc ctcaatcact gttttcaggg cattgttctt taaccaccaa 3960 tcagacactg ccgaagctac gatgtagtaa gcaccgtcat ctgtgaagat tggctcagag 4020 atacatggtg aaatcatagt ggtgatgata gagccattat tcatgtaata tgttcttttt 4080 tcaaccttat caagaagctt gtgaaggtct gcaaagagac ttaccacctc tttatggtca 4140 atatcttttt caagatttgc tctatacttt aatagtgcag catcaacctc tttatcatta 4200 aatttgaagt actggacttc ttcattcaca catacactga tgttgttggt gtttatcgtc 4260 aatttgcaca gtttgactgg agagttaatg ttggtctgaa aaagtgctac acagccttct 4320 acatttccga aatgcttgac taatgtaact tctggttgga tatgctcatg ttcttctgac 4380 attacagttt cctcattaaa tactttttaa gttgtgtgtt aggtttctca aaaacttcta 4440 tagaagatat cttaacagac tcaccacgca taccagtatg tttgaatatg tagtactcta 4500 acatccctat ggactttgct ggtaagtcat gcttgacata caccgttaca gaatcttcaa 4560 attcaacaac ttttgtacca tctttgtagt tgaactcttt aggatggttg taattgtcta 4620 tgtcatacat taaaaagagt cttttccgtt gcatatcaag cttgtttgtt aaagactctt 4680 tgatatctga gaagtttttg taaactgtga catggagctt actcatcgtt aaactctctg 4740 acgaagaaat agttgacttt gttattacct accataaggt caaagcaggt attttcaaaa 4800 ggtttgttgt aggcatcgta tgaaatatat gggtcagcta cttgcttagt gcttaacttc 4860 ttacttgatg ggtttaagag gccaccatac agcttcttga tagcctcttc tacaacagac 4920 cagataattt cgtcagtaat ttcttcgtac aggtcaagtt cacgctctag tgagaagtca 4980 caaataggga aaataacctg tactttactc attaacgaat aacctgtgtg acttgaattg 5040 cttcagttac tgataagtca cctactttct cttctttgat aaaggtgaag gtgagttcaa 5100 caggttcttg ctcttcttgc tcaactggaa tgccattaag ctcatcaaag attgattgag 5160 ttgtccagtc ttctccacca tctggatagt ggattgcata aagaggaaat tccacgtcat 5220 accagccaaa tacagggcaa ccgtctacat catcacgtac tcgtcgaaga gtgttgcctg 5280 attctgtaaa tcgaaggctg agattaccat tacactcaaa aattactgca tcagcaggcc 5340 caacattttc tcgaccatca tgaccaaatg tgtcaacctt tacttgaatc tcgttattca 5400 taaaaacctc tctctgcttg ttcgattttt gctcttaaat tttcgcaatg ctgtagatgg 5460 tagttagctt cttgttcatg tgttgctaac tgcctttcca gaacagtttt atagtaacct 5520 gcaagttgct cttttgtcaa tgggtgctct gaaataatat caagtccact ttcgccaaga 5580 gggttgaatt gtccatgctc attgacatgg taagtttcaa acttgaacag accgttgtta 5640 gcagattcaa taggtgagag ttcagccaac tcaacagtga acattgtgtg taccctatca 5700 ccaactgtaa caatgttact accaatgatt ctcaccattg agccattgcg agtgtactta 5760 aaagttccaa agtcactagc ccaaagtttc atagttttct ccttagaaaa taaaaaggct 5820 cccgaaggag cctatgaaga tattactttg ggtatacact gtcaagataa atgtcagctt 5880 ccattctacg tctgtttttc aggccgtttg aagtgacctt cttacctttg actgtaacct 5940 tgttccacca ttgcatagct tctgcacaac ctaccttatt accatcattg tggcgcttga 6000 taaatgtaga atcctgcata gctgtgatac cgatgttgta tgtttcactt acaagtgcat 6060 cgaactcgtt ctgagtagtt ggagccttga tagctttatt cactgctgca acgaatttct 6120 caacatctgc gagaagatac tgttcagctt gttcagccgt gattttcata cccatcttaa 6180 caggttttcc gtcaatacgg attgtaccat acccgattgt tgggattccg gcagaatctt 6240 tgtaagcctc taacttcaga ccttcaaaga acttaatggc ttctaagcct tttcttgaga 6300 gttgcattat gcctcccctg ctgggttaac tgtaactgtt gctgcattag atgtcacaga 6360 gccacctgca ccagtaacta cacaggtgta gcttcctgaa tcagcagttg ttgcaccttc 6420 tttggtgtaa gttgcagtag ttgcatcgag gatgtcttcc tcacctttct tccattggta 6480 acctgtcgca cctgttgcca caacattcag tgttaaggtc tcaccttcag tgatttcttg 6540 attggctggt tgctgagtaa ttgctggtgg cttaatagca tctttcaact tagcattcag 6600 cattgagaac ggcttaactc ttgccagcca ttcacagtaa attgtgtcaa catctttacc 6660 attgataatt gcatattgta agtccatgaa gaagtcagaa gttctcattt gagcaccaat 6720 gctgtaaagc agctcatcac taaatggaac tttatagtct ggtttgtagt caaacttctt 6780 aacttctgca atgtcagctt caggccaata cgaactatag gtgagtggta aaatagactc 6840 ttgatatgtt ctaaaatcat atctcttacc agtctttaca tttgcaatga agcccttcac 6900 aaactctttg aagtctgggt aagtctttgc tttaatcatt tctttttagc ctctaaatgt 6960 gcgtttagtc taaaataacc tttgtggtgt ggagatacat caccaacaac aacaccttta 7020 accttataac ctttaccaat tacatactgg atatccataa ggaatctacc aagaccaaca 7080 atatggaaca ttggatataa tagattctca gagtctggaa tattgatttc aggtttgtag 7140 gtgcgttgtt cgattgttgg tgtaatatct tcttcaggcc atttactgag tctccatgtc 7200 aaaggtgttg ggataacatc attaaggttg tatctttcac cattttgcac ggaattgata 7260 taggattgta ggaagcaata aaagtctttt gcactcttag ctttcattaa atatccttat 7320 aaatagttaa aggggcatca gcccctattc acacttaagc tttggtctta agtaggttct 7380 taagtttttt aagattcata ctctcatcaa acttaaaacc aaatacattg acataatcaa 7440 tgaactcgga tttcttactg agtgacaatg cgtaattcat atcaaagttc ttacttgttt 7500 ctgcgtctgg actagagttc acatcaaatg agatttctgt aggctcttgc ttactttttc 7560 tcatattatc agtcaacaaa catgtatagt ttgctggata gaagtttaca ctaccatcaa 7620 cgtagctgta cttattatta tggataaggt tggaaaatac cttaaggaat gtttctgagt 7680 cgtggcaaac aagtttgaat tggctgctct ctccaagtgc aactaatgag tacggacttt 7740 cactatcgaa gtcatagttt ggattcgatg tcgtcactgt tacacgcatc aagttattag 7800 caatagtctt ctgtttaaaa gaatttggaa catagtgaag acctctacca cactgattga 7860 ctagctcttt aagtaggtca tttgcatacg gtgaattaat tgatacagag ccatcactat 7920 tgtaattaaa acttactgtt aacatttttc agttctcctg cgatttataa ttttgctgca 7980 atagagaaac gaagtaaact cactggaaga aaccattaag tgttgttcac tagattgagc 8040 cattgccctg aaatacattc cagtcaaagc atcatagtga ctattctttc caattgaggt 8100 tatatagcca tgactctttg cagtttgata taaatctata atatctgcga tttctaattg 8160 ggaactacac atagataatc ctcttgtctt ctgtaatttt gaaagaacag gtacagacca 8220 atttcagagg atgaatagta gattaactct accctttttg attccttaca gtgattggta 8280 ttcttacctt tcttaggttt atttgatttt ctcattaagt ctcctttgta gaacaaggca 8340 gacctacagc tttaagtaag atggtacagt aatccataca acaagtcaag aactattgca 8400 agttaaaaaa gttgttgaca aggtacttga caaggtgtag gcttataaat acttagaaga 8460 taacttaaaa ggttcctcta aaaaggctat ttaaacagat atctttctgg atatctatct 8520 tttatcttaa aagcctttta agaggtctta taagaggcat accaatgaaa aacagaacaa 8580 acaaaggcca gtttaaaaaa ggccagtctg gaaacccatc tgggagacca aaaggctctc 8640 gtaacaaaag ctcactcgta aaagctcaac tgaccattga taattctgct gagtttgctg 8700 caaagctgtt tgaggcgatt gttacaaggg acgctgctaa gcttgcagag ttcggtttga 8760 cgactgccga tgtaaacctg aagtcgatga tggaagctgg taaaactatc atgacccact 8820 cagcaggtga gatgaaagcg attgcagcag acactaagaa gacccctgat aatggtggtc 8880 agtcacagac agataacaaa ccaacgttct ctgctgtagc gactctcaaa aaataatttt 8940 aaaaggtgtt gacagactct acagagttgc tctaaagtct gtcacatcaa acaacaaatg 9000 agagaagaga gtaaaatatg agcgaattat ttaaacatgc gcaccttcat gcaggtcgaa 9060 ctgaaaatgg tgctgtaaac catacttcat caatgtctgc tcttgtggac ttttacaaag 9120 ctgctggttc cagccgtagc aatgtagaaa tcttaccaga cctgttctac aaagctttgc 9180 gtgaggatgt tgatgttgca gttcgtattt tactgcatat gcgagatgta cgagaaggta 9240 tgggtgagcg taaagctttc cgaactgttt tacttcaagc gattgaagat aaagttttag 9300 agcctacaca ggttcttcgc attatggata agattgcaga acttggtcgt tttgatgact 9360 tcaaaatctt cgtaggtact cgttttgaga cagatgcctt caaacattta gaagcagcat 9420 tactagaccc tgcaacagca ggtttagcgg ctaagtggtt accacgagta aaaccacgcc 9480 acaaacagtt tgtaaaacgt ttctgtaagt ttgcaaactt aagtgagaag gagtatcgca 9540 cactgttatc tgcactgtct gatacggttg agcaaaaaat ctctgctaat gagtttggta 9600 agattgacta cagcaagatt ccttcactcg ctgctgcacg ttaccaaaag ctctttaacc 9660 gtaaagatgg ggaacgttac aaagcttaca tcgagtccct ctcaaaaggt gaggttaaga 9720 ttaacgctgg tgctgtttac ccatacgatg tgattaaatc tatcaagtat ggtaatgcag 9780 atgttgctaa tgagcagtgg aaagcactac caaactggat ggcagaaggt gagaacatct 9840 tgtgtatgac tgatgtttca agctcaatgt cttgggtgaa tcttggttca atcactgccc 9900 ttgatattgg tgtatcactt gctttgtatg tagcagaacg caacactggt tgctttaaga 9960 atgagttaat ggtttactca acaaaccctc acttcatcga actgagtggt gatttacgaa 10020 accgtcatcg tcaggtaatg cgtcatgttg aatatggttc aactaactta caagcagctt 10080 ttgaccgtat tcttgaggta ggtaagcgaa acaacttgac tcagaaagat atgccaagta 10140 agcttattat cttctctgat atggagttca ctcaggttga tggagctaac ggtcgtacaa 10200 actttgaagc aattcagagt aagtacaaaa aagctggata cgaaatgcca caactagtat 10260 tctggtactt agcaaaccgt aatggtactt gcgaagtatc tgttaaggat aatggtgtag 10320 cgatggtatc agggttctct ccagccactt taaaagctct gcttggtggt gagaagtttg 10380 acccaatcag cgtaatgctc aaagcagtaa tgattgaccg ttacatctgg taaaaagttt 10440 taaaaagggt attgacaatg tgtttgatac cctttaatat gttctacata gaaacgaaat 10500 gagagctttt ctaagatact gaaaaatatt ttaaaaaagt tcttgacaac cactaaaaaa 10560 taatgttaaa gtggttacat agagtttgaa aagtttatct ctgtttagct cagcttggta 10620 gagcgttccg tttggggcgg taaggccgga ggttcaagtc ctccaacaga gaccaaatta 10680 atgttccagt agacaaaatg gtaaagtcac cactctttca aagtggatat ttgagggttc 10740 aaatcccttc tggaacgcca gttttgacag aagaccaatt gcagcaaact taatcttatt 10800 catctgacac gaaatcggac aaagaagaga tttggtctgg tcattaagaa ttgcgggtat 10860 agagaaaggg cgtctcacat gtctcattag catggtatcg gcaggttcga ctcctgcacc 10920 cgcctccaaa tttacagaag accgtttaca gcaatattta ttaaattttt agcggaaaaa 10980 ttgaaagata cggtctggta atactaaaca tggttaggaa gcacataagg tatgtgcggt 11040 cgcctgttaa gcgaatggca cagggttcga atccctgact aaccgccaaa tttaaatgtg 11100 tcgttatccc gtagatggta gcggtgggga ctgtaaatcc cttgtcattg agactcggta 11160 ggttcgactc ctacacggca caccaaataa aggttatgta gtttaatctg gttaaaatac 11220 tcccctgtca cgggagatga tgagggttca aatcccttcg taaccgccaa attataataa 11280 gtatttcaaa atgatgtttg agagggctat ttagcccctc ccttaaagag ttcttacgag 11340 tattctttaa aggagcagaa ggccaaattc agcaagttac ttaaaatttc aagccaattt 11400 aattttgaaa aatttaaaac ttggtctggc tcaacaattt tacagaagac cgtttacagc 11460 aaaacttaaa caatctattt ctccggtaaa gaaaaggcga aggttcgatt ccttcactcg 11520 gcagatgtcg agttggtgta atggtagcac ttaagatgat aaacaacggt ctggtaatta 11580 tctttgtagg tgagctatta agcttacctg tgagtatgcg accacaaagg gtgaaagggt 11640 aaattctaga tttaaaccca acaaggcaca tcgctacaga ccttaagcaa gtctgggtag 11700 cttatctaca aagataattt aatggaagtg tagcagaatg gtgatgcggc agacttttaa 11760 tctgacaggc gatgggttcg aatccctcca cttctaccaa tatggttcag tcgcagataa 11820 ggtaatgcag gggtctcata agccctatga atgtgggttc gattcccatc tgaacctcca 11880 agctggtata gttaagaagg ttataacact cccctgataa gggaacatcg gtggttcgat 11940 tccacctacc agtaccaatt tcaataaagt tgttgacatt gagatgtgac tactttatag 12000 taactgaaaa tgattcagca tatgtataat tcaaacacca atacatgagc attagacgta 12060 atgcaatgtg gggaaatttg ttgtacatat tctgaatagt tttcgttgcg tagcgtctat 12120 tttgcaaatt taaaataaat gcaaacgaca atgtttttct ggcagtagct tgataggcta 12180 aacaccagtg aggtcttcca atccctcatc aaagaatttg gcgtactagc ccacggtatg 12240 attaataagg tgggcaacat tttaaagggt atcccttatt gggcttggct taaatgcaat 12300 gagtaccctt taaaatgtta attacggggc gtatctcagc ggtcttctaa accgccgtta 12360 accgagtaag tggagtatgc gggttcgagt cctgccgcct cgaccaattt agttagcaga 12420 ttgcttagac gaccttatcg ggtctccgtg taaggaggtg gtctgtatct catgttttcc 12480 agaacatgta aataatctga gaaggggctt tacagtgttg tgaaaatacg gtaagcttcg 12540 aattacaata tgggacgatg ttaagactct aaggcatgag caacggcttc caacaccgtt 12600 tcaaaggggt tcaactcctc cgtcccatgc caaagtctta ttaggggtag gtagcggcta 12660 atggtagcca aactgtcttg aaaacagttg ccactgtaga gatacggtga gggttcgact 12720 cctttactta ccgcctaata agatttacac cgtccatgct tgctcgcatt ttcggtttat 12780 ccatcgtgta ttgtcgtaca cactgataaa gcacctagta ggtgtcacgg agaagagata 12840 atatcaagct ctccaaaggt tctagtcacc ggattaaaca agactatgca aagacctatt 12900 taggtctttt tagagggctt ctaaggttat tacagcttgt tgtggaacct tgttgcttag 12960 gttcttagaa gttctctaaa aagatttaat gggagattga cggtaattgg taaacctacc 13020 atccttagaa gttggtgttt gagggttcga atcccttgtc tcctaccaaa ttaatgcagg 13080 tgtagcaaaa tggttatgcg gctgactctt aatcagtaag acgatgggtt caattccctc 13140 cacctgtacc aaacattggg gatgtagttt acatggttaa aacataagtt ttgcaaactt 13200 aagtacaggg ttcaattccc tgcttctcca ccaattagtg catccatagt ttaaacggga 13260 aaattacagt cttccaaact gaggttgagg gttcgattcc ctctggatgc tccaaattaa 13320 tgctcctatc gtataattgg ctattacggt tgccttgtaa gcaacttatc agggttcgaa 13380 tccttgtggg agcaccaatt cagaggtcaa gtgaaagacc gctggtgtca actgaagacc 13440 gtaacaaatt ccacggagtt gagttagcgg cacaacttca gacctcttcc atactcgctt 13500 agtttatatg gtaaaacatc acccttacaa gatgaagaaa aaggttcaag tcctttagtg 13560 agtaccatgt tccagtatcc caattggcag aggatgcaag ctcaaacctt gtactagtga 13620 cggttcgaat ccgtcttgga acaccaattt tgcaggatta gttcaaatgg atagagcaac 13680 agtctacgaa gctgttaata ggggttcgaa tcccttatcc tgcgccaagt taaagaggct 13740 atttagatga gatatttgaa gtatgcttca tggattttct tagctttgtt agaaccattt 13800 gcagcaatct tagcagttat cttagcacct tttgtagttc cattctacag tgagaagaaa 13860 ggacatctac ctttcgggtt cagatggatg gaaacttatg acaacccaat tgatggcgat 13920 gaaggtcatg tcaaaagatg ggctaagatt agaaagattg gtaagcttgg tatctatatg 13980 cagagagtcg gttggctctg gagaaacaaa gcttataact tctcttacca tgtgttagga 14040 agagatgtaa aagatgttac taagtggaaa ggtaatatca atgtaagttc taaccctgaa 14100 gataatcaga caggttatct cctaatgtgg aacagtaatg cttgggggtt attcgctttt 14160 gtaccatcaa ttaaaatctt tggtaaacaa ttctactgga gaatttatgt tggatggaaa 14220 cttaaaagtg ttgtcccaga agaaagagca ttctcaaggg aaagagttat gttggcattc 14280 tttatccatc cactaagaaa ataaagattt aaaggggatt agtttacaag gttaaaacct 14340 cggtctttga aatcgaagaa gttggttcaa ttccaacatc ccccgccaat gctccattac 14400 tccaattggc agagaggcca gacttaaaat ctgtgttatg tatcggttcg aatccgatat 14460 ggagtaccaa atttagcggt atagcataac tggcaatgca gcagtctctg aagctgtctt 14520 attaaggttc aaatccttat gccgctgcca cttctaagga ttcttacgag agtccttaaa 14580 tgtggcctta tcataaatgg taatgaccca tgctgtgaac atggtctata cgggttcaaa 14640 tcccgtaggt caccccaatt tatagtccaa gtagcttata tggttaaagc gcgtgtctga 14700 aaaacatgag aagagggttc aaatcccact gggctaccaa tttcaaaggt gcttaatgaa 14760 agagatgaca gaacaaggta aggagatttt taatctctta aaaaccggta gagggttttc 14820 taatcccctt attactggtg cagcagttct cggtggaacc gtagctgcct ctacatcact 14880 tgtaagctct attagctctg taacagaccc tacaataaag gataaacttg ttgctgctgg 14940 acttacaaca gttctgctta acagctttac aacgagtctg acaagcacta catcaaccac 15000 taagacattg gcagattacg gtcaaaagtc cattgatgag ttttcatcac gtatgcaggt 15060 agcaaaagga tattccaatg ttatgggtgc agcaggagag caagtcggtt gcacaccatt 15120 tagtggtatt atgggtgttg ctacagagta cggtcaaaaa gctattgaca caattaacag 15180 tacactagat ggtgttaatg atgtattaag tgacttacaa gatgctattg ataaaggtct 15240 tgatactgtt tctgatttag ctaatcaagc tgttagtaag attaatgaag gtatctcgaa 15300 gattacagct tatgcagatg aagttgtgca gatgattgaa gaagaagctg cccttattgc 15360 agagtacctt aaaacgaata tcaatgggtt cttagcaggt atcttaccag actggtttga 15420 tgatgcttgt aaaactggtg taattgacac tattgcaaca ccagaaatga agaacgcatt 15480 acagaaataa tggaagatta accctaaaag gtaagggagc agtttgctaa actgccagta 15540 gctgagaaat cggtgtacca gttcaagtct ggtatcttcc tccaatttga atccgtgaca 15600 gaaatggcta tgtacctgtc tgcaaaacag gtttataagg gttcgagtcc cttcggattc 15660 tccaagttat tttactctct ctcaattaaa ataataata tgccctgcta taagtattct 15720 tcctttcgct accgaagagt atttttagac agggcttttt tacagagtta tactttataa 15780 gaagacttat acagtatgtc tctgacgtac attgtggttt ctccttgacg gtctctgtac 15840 cgtctttttt aaggagaaac atggttaact atttggagaa aacataatga gcgagaccgt 15900 ttataaagaa ctttatgaag ctaataagaa gctagaattt atgcagaata ctatcatggc 15960 aattgcagac agattatctg tagcaacagg tattgatatt aaagaagcgt ctatggatgc 16020 acttcttgac gctgttgatg caaagttcga agttaagaaa gaagagactg ctacagactc 16080 tgaataattc tatttgcgga ggtgcttaat ggacttaaat gctgttaagc agaagcgagt 16140 ggaagatgtt aggaaagtcc tagctggaga gttggggctt tctgatgaag taaaagaaat 16200 tatcaaatca ttcggtaaag acccctctaa attccttcca actcaaattc tgactttatt 16260 aagatacaca ccagaccaag ttagacttat cttcaaattg atgactgata agaactatgt 16320 agcccctcag ccgggttctc aagaggtctt tttaaatact aatgctgact tggttctcta 16380 tggtggtgct gcgggtgctg gtaagactgc tgcattgttg atggattctt taagatttat 16440 tgaagaccct aactataatg ctgtgtattt ccgtcgaaac acgacacagt tacaaggtgg 16500 tttatggcct gctgcaaaga aactatttgg taagtttggt ggggttcctc acgagcagaa 16560 gatgactatc acattccctt ctggggccac tatcaagttt acctacctag aacttgaaaa 16620 gcacgctgaa ggtcatcagg gtattgaata ctcagctatt tactttgacg aaggtacgca 16680 cttctctgct tcacagattt catacctaca gactcgtcta cgttctgggg ctgaaggtga 16740 ttcatacatg aagatttcta tgaacccaga cagagaccat tttatttacg attgggtaga 16800 gccattctta gatgaagaag gttacccaga ccctgaaaag tgtggtcgta ttcgttggta 16860 tgtaatgaat gatggtgtta tggtttctga ttgggagaga gacaagattc ttgaaatgtt 16920 tcctcttgag attcctcaaa catatacatt catctctggt acgattgatg ataacccaat 16980 tcttgacttc ttagaaccta aatatcgtgg taagttggaa aacaacaccc ctgtaaacgt 17040 tgcaagactt cgtttcggta actggaaggc tcgtgcagaa ggttcaaact attggcaaag 17100 acaatggtgt gaaattgttg attcacttcc agaagatgta ttcgatgtca gagcatggga 17160 cttagcagca actttaccat ctgagattaa ccctaatcca gactggacaga caggtgttaa 17220 gatgggtaaa tctaaaaaag acggttgcta ttatatcatt gatgtagtaa gatttagaga 17280 tagaccctct ggagtcgaaa cacaaattaa tttgactgct gaaagtgacg gtaagcgaac 17340 tggtattttt atccctcaag acccaggcgc tgctggtaaa tcctacgcaa catccctcat 17400 caggaaactt gccgagaaag gttatcgagc aagggctaaa ccaacaaata aagataaagt 17460 tacccgattt gcggggtttt ctgctgcttc tgaagctgga cttgtaaaag tcttgagagg 17520 tagttggaac gaagcttact tccaagaact tgaaggtttt tgtggtgatg gtaaaactaa 17580 agatgaccaa gtggatgcta ccagtgatgc tttcaacagt cttaacgaag ttaaattatt 17640 caagccacca tcaatgggtg ctcacacaga cttagtgaga ggaaacccat atgaggggct 17700 tagacgttga tagctaggtg agaagaatgg cagatattac agaaacacaa gaaagcttac 17760 caccatttag aatgggtgaa gtaggttctt tgggtctgaa ggttaagaat ggaagaatct 17820 atgaagaacc tcgtcaggca ctaaggttcc ctgaaagtat taaaactttc caattaatga 17880 tgcgtgaccc tgctgtagca gcatctgtaa atattattaa gatgtttgtc agaaaagtca 17940 actggagatt cgtacctcca aagggaaaag agcaagaccc taaaatgctt gaaagagcag 18000 acttctttaa ttctttaatg gatgacatgg agcatgattg ggcagatttt attaactctg 18060 taatgtcatt ctgcacttat gggttctgtg ttaacgaaaa ggtttataag aaacgtcagg 18120 gtaaaaaagg aaagtatcag tcaaaatttg atgatggtct aattgggtgg gctaaattac 18180 caatcagaaa ccagtcaaca cttgataagt ggtattttga tgaagacttt agaaaagtta 18240 ctggtgtcag acaaaatcta agaaatgttt cacatattgc tggagcaatt aatcttggag 18300 aaagaccact aacaagaaaa cttccacgag ctaaattcat gctgtttaag tatgatgatg 18360 agtatggtaa cccagaaggt cgttcaccat tacttaatgc ttatgtaccg tggaagtata 18420 aagtacagat tgaagagtac gaagctgttg gtgtttcaag agacttggta ggtatgccaa 18480 agattggttt accaccagat tatcttgatg aaaatgcaga gcctgaaaag aaagctttcg 18540 tacaatactg caaaactgtt gttaatgata tgattgctaa tgacagagca ggtttaatct 18600 ggcctagata tatcgaccca gatactaaag aggatatttt tgagttctca ttagtttcta 18660 gacagggtgc taaagcgtat gacacaggtt ctatcattga cagatattct aagcagatta 18720 tgatggcatt tatgtcagat gttcttgcta tgggtcagtc aaaatatggt tcattctctc 18780 ttgcagattc taagacaagt ctattagcga tgtcagtaga tattctgctg aagcaaatta 18840 agaacgtaat taaccgtgat ttagttgcac agacttatgc tcttaatatg tgggatgacg 18900 aagaacatgt acaaatcaca tatgatgata tcgaaactcc agaccttgaa gcaattggtt 18960 cttatattca gaagactgtt gcagtaggtg ctttggaagt tgacaaagag ctatctaaca 19020 aacttagaga gcatattggt ctccctcctg ctgatgagtc tcagccagta tctgaaaagc 19080 tttctccaaa tagccaaagc cgctcaggag atggttataa gaccgctgga gaaggtactg 19140 caaagacacc ttcagcgaaa gacccaagca cagcaaataa ggcaaataaa taatggctga 19200 aattatctct atttcaaatg ctacacgagt gcattcgtat aggggtgtcc ttatcattac 19260 tgataagcta tctgtagagg ctggctcaag ggtcagcctt tcaggttatg ttagtgatgg 19320 tggagtctct gacgttttca ctatttgcag gttacttgat gcaccaatga gtggaaaacc 19380 gtttatttca ggaaattgta gtgaaattgt taaaattcca tttgacagtt catgcctttt 19440 gggtgtaaag ttatataact gcgagaataa acgtatcaat gttaatagca ttgaagccgc 19500 tttcattacc ctcgacactg catttcaatc cccaatgaca gttaacaaag aaacaaacag 19560 acttgaatac attttttcac aaaatgatta caaagtactt gtcaaaggta aagtatatga 19620 tatgattgta aatgtggtag atgaatctgg taaccattca acagtcctta aacaaaaagt 19680 aaggtttaat taatgggaac attaactatt gatggtaaga ataaaatcct cgccacgcta 19740 accccaacga ctattatttt acacaatgta gacccaacgg cagaccctac agcaaataag 19800 gttactcagc cagtggctat ttatttttct gaaccggata atggcttaat tgcctcagaa 19860 gacacagtta atattactgt tccagcttct gcaacggtct cacactatag cttgtgggat 19920 gctaacgata aatgcgtggc aactggtgct cttagcaaac ctcaattctt tgctgaagaa 19980 ggtatctatg ttatctcttc agtctctgta gatttaaaca aatagggtga gtaaaaatga 20040 gttcgaatat cttcagactt gctgatagat tattcaacca acctttacta gccactgaat 20100 cattagctca ctcagcagca acttatgtga ataacagatt gctgggtgat gtccaagcag 20160 cagtaaactt tgataaaccc aaaggtgaag caagaagtct tttaaaagta aaagatgaca 20220 ttgctattat tcctattatg ggtggtttaa cccatcgtat gacattcatt gatgcaatgt 20280 gtacaggtgg attaagctct tatgaaggtt tgcgtagagg ctttgacgaa gctttagcag 20340 atgagtcaat taagactatc gttctgcata ttgattctgg tggtggtgaa gcttcaggtt 20400 gctttgaatt agcacgtcac attatggctt caagaggcca aaagaaaatt attgcttatg 20460 tagatgagtt cgcttgttcc gctgcatatg ctcttgcatc ttctgctgaa gaaattattg 20520 catcaccaga tgcagatgtt ggttctattg gtgtaatcat ggttcatcag gaattaacta 20580 aggcatttga aaagaatggc gtaacaatta acgtcatcaa agctggtgag tttaaaggta 20640 tgggttcacc attccaagca ctctcagaag aaagcaaaga aagacttcaa aagagaatta 20700 atgataccta cgcaaccttt acaggttttg tagctgaatc tcgtaatctc tctgaagaag 20760 ctgtaaagaa tactgaggcg aatgtttatt ctgctcagga agctcttgaa cttggtttaa 20820 ttaactcaat catgtctcaa gatgatttct taaattactt acaaggttct gaagaggctc 20880 ctgtaagttt aaacgttaac aattaaggtg aagaaatgac tgaacaagaa aagcaagaac 20940 tagaagcttt gcgtcttcag gttgctcaaa tgaaagctaa agaacaggaa gctgctttgt 21000 cagatttgac taataagatt tctgcttctg ctgaagcttt tggatttgat gcaaaagaag 21060 ctgcaacgac tattttaggt gctggtcttg ataaccctct gagtgttctg tttatgaatg 21120 ctatggaagg tgctaaccag aaacttaatg aaactatcgc atcccatgct tctgcaatgg 21180 aagaaaaaga atcagaaatt accaagctga aagaaactgc tggtgctgtt cttgaacatt 21240 ccaacgctat ggaagaagtg ggtaatgacg gcgaagctga tttggttgaa gaagaaaaag 21300 aaccagctaa gaatgcttcc gaagacaccg ctgaacaacg caaactggct ctccagaatg 21360 ctctaaaatc tcttatcaaa taaggaacac aacaatggca tatcaaggtt ttactaagtt 21420 aggtaaaaga gaacctctga atgatatcat tctttgggaa caggttaccc caacaggcca 21480 ctctcgtaaa gagtacaccc cacaggcatc tacagaatat cgtgtaggtg aagttctgaa 21540 agcagacggt actaaggtta cagcagggca agaagctcag gctgactctg tatgtatcgt 21600 taacttctac gcagactcac aactgtctta tcatggtcag ttgaaagttg ttggtattta 21660 ccgcgacgca gaactgaaag acatgcttac tcttgaagca agcgttgacg ctgccgaagt 21720 caagaaagct ctggctgcca aaggtattga tttcgtacca actggcctgt aataaaaata 21780 ataagacatt ctggagaatt ttacaatgtt gactaattct gaaaaaagca gatttttcct 21840 tgctgacctg actggtgaag tccagtctat cccaaatact tatgggtata tttccaactt 21900 aggtctgttc cgttcagcac caatcaccca aactactttc ctgatggatt tgactgattg 21960 ggatgttagc ttgcttgatg cggtagaccg tgatagccgt aaagcagaga ctagcgcacc 22020 tgagcgtgtt cgtcaaatca gcttcccaat gatgtacttc aaagaagttg aaagcatcac 22080 tcctgatgaa attcagggtg tacgtcagcc aggcactgca aacgaactga ctactgaagc 22140 tgtagtacgt gctaagaagc tgatgaagat tcgtaccaag ttcgatatta ctcgtgagtt 22200 cctgtttatg caagctctga agggtaaagt tgtcgatgct cgtggtactc tgtacgctga 22260 cctgtacaag cagttcgacg ttgagaagaa gactgtttac ttcgaccttg ccaaccctaa 22320 tgctgacatc gacgcttcta tcgaagaact gcgtatgcac atggaagatg aagctaagac 22380 tggcactgta atcaacggtg aagaaattca cgtagtcgtt gaccgtgtat tcttcagcaa 22440 actggttaag caccctaaga ttcgtgacgc ttatcttgca cagcagactc cgctggcttg 22500 gcaacagatt actggttctc tgagaactgg tggtactgac ggcgttcagg ctcatatgaa 22560 caccttctac tacggtggtg ttaagtttgt ccagtacaac ggtaagttca aagacaagcg 22620 tggtaaggtt cacactctgg ttggcattga tggtgtagca gcaactgttg gtgttggtca 22680 tgccttccct aacgtatcta tgctgggtga agctaacaac atcttcgaag tggcatatgg 22740 cccatgtcct aagatgggtt atgcaaatac acttggtcag gaactgtacg tatttgaata 22800 cgaaaaagac cgtgacgaag gtattgactt cgaagctcac tcttacatgc tgccatactg 22860 cactcgtcct cagttgctgg tagatgttcg cgctgacgct caaggtggct aataaactta 22920 ggagggttat gaatgtgtta tacaggcgac ccagccaata accctcttga tagagtaaga 22980 atcctctgca cagacactaa taatgatgaa attcttattg agcagtctgt gctagagtgg 23040 ttctatcaag aatctggaaa ggatgaaaag aaagcagcca tcaaagctct taaatattta 23100 ctctttcaag tagccaagat gggagatgag aaggttggtg gtgtttactt acgtaactct 23160 tccagattca aatctctgaa agctgtttat gacgaccttg ttaaaagctc tgtttcagga 23220 ctaccctatg caggtggtat taaccagtgc gacattgata tgcgtcgtca gaatccttgc 23280 tctgtcaaga aatacacaga atatggtgat gctgccagat acgaaggcag agattactgc 23340 aaccgtgtta atggcgtatt tattatcgag cgagatgaat aatggttaaa agggttattc 23400 accccgctag agcaaaatta gtcggggcta tgaagaactt gcaaacggct aatgctcaag 23460 ttgggtattt tcaagaacaa ggtcaacata gctctggttt ttcttatcct gctttaatgt 23520 atttacaaga agttattggg gttccttcag cttctggtaa agtatatcgt aggttgtttg 23580 aaatcactat gatgctaaac aaacagacct tgttagagca gactaagaag aatctatata 23640 agcaacttag cagtctcaac acagaccctt caaatacctt agaagcattt gcaaagaatg 23700 ctcaaaaggc aattaaaaga ggttttggta attctgctat cctccctcct aacgcacctt 23760 ccacagtcaa gaaaaaaggc tttaatgcac ctcttgttga gacaggtgac ttaagagata 23820 gccttgctta taaaatttct actaagaagg gtattaagaa atgagactct taaacagaca 23880 cagctttata gtaaagcgta aagtctctga agacggttat tataatgatg atggtgattg 23940 ggtagcttca caagatattg tagaggttaa ctgcaaaggt aatatccagc catatatcaa 24000 aggttctgta aagaatggta cacagattgc tttaccggaa ggtattaggc ttaccgatac 24060 aagaatcctg tataccacat ataaacttag aacttcagat gatgtagagt ggaatgagtc 24120 tgacattgtt atgattgatg gtcatgagta tgaagtattt atgactatgg attggtcaca 24180 gcaattagcc catacgtccc attatgaata tatcattatt agaagggata aaatgaatgc 24240 agttagaaac agcagaactt gaaaaaggtc tagttagaac cttagtggat gttattggtc 24300 acagactaac tcgtgataaa aataatagac caaatgtaat tagagcttac ccttctgata 24360 actcaaatga caaaggttta aaacctgacc agccatttat taccgtatat tgtcaagatg 24420 ctgcaacacc ttatggttgg gttcttgata agtttgttga ggatgatgta gtttgctaca 24480 gaattgcttt tcagattcct gtactaatta cggtgaatgg taaaggtgct cacagtatta 24540 tgcttgagct taaacaacga ttagagatga gttcagtcag ggatttaatt cttgaagaaa 24600 caggggcaac agtcttagac actggagcta tcccgaatga ctatacttat cttaatacag 24660 atttcgaaaa ttctgcgcct cttgttgtaa ctcttgtaaa aaattcagtc ctgaaagatg 24720 aacgtggagg tattattgaa cgtgtcattg ttgatggtga attagtttac gaagaaggac 24780 aagagccacc agaatatact atccatctag atgtagactc caaaggggta aaataaatgt 24840 ggaatccaat tgttaatgta gatattacat tgaacaccgc aggaactaca agagaaggtt 24900 ttgggctacc actattttta gcttcaacag ataactttga agaaagagtc cgtggttaca 24960 cttccttaac tgaagttgct gaagatttcg atgaaaactc tgctgcatat aaggctgcta 25020 aacaactttg gagtcagact cctaaagtaa cccagcttta tattggtaga cgtgctatgc 25080 agtacactgt atcaattcct gactctgtta ctgaaagtac cgattattct ttgactgtag 25140 ctgtgagcgg tggaatctct cagccattcc agtatacttc aaagacatca gatactgctg 25200 agaaggtgtt acaacagctt aaaacacaga ttgaagctga cccaacaatt aaagataagg 25260 tcactgtgaa tgtaactggt gctggcggtt cagccacaat ggtaatcacc aaagctggtg 25320 tgaacgactt cgtgaaggtt acttctacag cacaaacagt gtcaattgca agtacaactg 25380 ctgacacagc atctactgct ttggcagcta ttgaagctta ttcaactgac tggtatttca 25440 ttgcagcaga agacagaact caacagtttg tcttagcaat ggcttctgag attcaagctc 25500 gtaagaagat tttctttaca gctaactctg atgtaacagc actgcaaggt acagaattag 25560 ctagtgcaaa tgatgttcca gcacagcttg ctaagagtca gtacactcgt accgtttgct 25620 tgtggcatca cacggcagca gaagactatc cagaaatggc atacattgct tatggtgctc 25680 catatgatgc aggttcgatt gcttggggca atgcacagct aactggtgta gctgcttctc 25740 tacagccagc taataagaga cctctgacaa gtattcagaa gtcagcttta gatgcacgtc 25800 actgtaactt tattgacctt gatggtggtg ttccagtggt tcgtagaggg attacttctg 25860 gtgggatatg gattgatatc atccgtggtg ttgactggtt agaatcagac ctgaaaactt 25920 ctctgagaga cttgctaatt aaccagaagg gtggtaagat tacttatgat gatactggta 25980 ttacccgtat tcgtcaagtc attgaaacct ctctgcaaag agcagtcaac agaaacttcc 26040 tgtcatctta cacagttaat gttcctaaag cctctcaagt tgctttagca gacaagaaag 26100 ctcgtatcct gaaagatgtt accttctcag gtatcttagc aggggctatc ttggatgttg 26160 acttgaaagg tacagtggct tacgaataat agaggtaaat tggaatggct atgtatcagc 26220 aatattcccc taaagacgtt gtatgtagct ggaacggcat tgctattgaa ggctttgctc 26280 cagattcatt cttacgtcta cagagaacat caccacttat cacaccagtt gtaggtgcgg 26340 gtggtcaagt tgctctgaca agaaatgcag acaagacagg tactattgag attgagctaa 26400 tgcagacttc tctctctaat cagatgcttt ctgcaattca agctaaacaa gacaatatgg 26460 aacttgaaga agatatctct tctaacttcg taatctacga cccatcaggc tctgttctgg 26520 caactggtat taatgcttgg ttgcaggaat taccgcaggt tgaacttggt cgtgaccaga 26580 actctaaaac ttggattttt ggttgtgaga agctagacta cacttctaca attccagcgt 26640 caagtgttta ataaatccta taagggggag actttaaaag gtcttcccct tttttgtttc 26700 ttttaaaagt attaaggaat cacaatgaaa acagaatcta gagtaattaa tggtaagaaa 26760 gtaaatatca ttctgcttgg ggcaagagat ggtattaaga tgtctatgaa gttgggtaaa 26820 attgttgttc caacctttgc acagatgcta tcaagtctga ctgataaaga taagaaagaa 26880 gctccaatgg ttccatttaa agaacttgtt gaagcttgtt ttgacagaat tgaagaaatt 26940 aaccttgaag aaatggctaa cttgttattt caaggggcaa ctgttgatga cttcccactt 27000 aatattgata cgtacttcca agcaaactac ggtgagttta ttgattactt agcatttgcg 27060 ctggaggcaa acttcggaag ttttttcgaa gcaagcattt tcaaaagcct aacttctcag 27120 taaacatggg taacactcta cagacaccac taactgatgc tgctgtagag gcaacctatg 27180 aagaagcaga cgagatgaaa tttgtgcttg ctatttatgg tatggaaggg tgtaaagaaa 27240 cacttgacca actctttgct atgacattct ctgatttatt atcattgaga caatttcttg 27300 aaattcagag gtcgtataaa gaggaaattg cttacaacga acttagaaga gcaggaaaaa 27360 tgtaatggca caatatacaga ttgatagctt cattgtggaa cttggtttca gtgaaaaggt 27420 aattaaaggc ttgcaaagag ttgagaagat gtctatgcaa gccgctcagc gtattgagcg 27480 aaatatgaat aaagcctttg atgtgaagcc taataaaagt tctcaggaag cacttaatag 27540 aattgtaaaa aatgctcagt ctgcttcggg cagaatcaat aaagcactca acagttcctt 27600 gaaccttgat tcacaaggtg tgaaatctct taagaaactt gaaactcaag caaaaaagac 27660 tgcaaagggt attaacaagt ccttaagaga tgctatgaag gttgacggta aaatcactat 27720 taagacaggt agagggagag gtggacgaaa tatccctcct gttggtggtg gagcacccag 27780 aggtccgaga gtagatgttg ctcagagaca aatggaaaga atgtttaaca acaacttcta 27840 ttcagggtta actcgtagac tggaaacaat tggtggtcaa ggtaaccaga tggcagcttc 27900 tttcagagga agcttacaaa gtatctataa taaatacaag ggtactggta aggttggtga 27960 gtatgagatg gaagttaaaa agctcatcga cgtaaccaaa cgttgggtta ttgcagaaaa 28020 tgctagacta aaatcagtta aagaagcagc ttggctacag gacagagcta actcatcact 28080 acgtcaatta gttggtggat ttgtttcagc ttatgcttta ctggaactat cacaaaagac 28140 tattgaggcg ggtgtaaaaa gacaatctgc acaattagcc tctacagcta tctttggagc 28200 agatacccag caagccagaa tgtttgctgc atcattcgca caccagattg gtcagaacta 28260 tacagatacc atgaagcagt actcaaactt tgctgctggt gctcaaccaa cgctaggttt 28320 tcaaggtact caagagttct ataagaacgc tgcaatgttt gcccgtatca gaggtgctac 28380 cgatgaagac ttgaaaggta tcatggttgc attccagcag atggcatcaa aaggtaaagt 28440 tcaggctgaa gagcttcgtg gacagttagg tgaccgttta gcaggtgctg tgcagctatt 28500 cgctgatgcc attgggaaga ctccacaaga acttgataag atgatgcaag acggtaaact 28560 tcttgctcaa gacgttctgc ctaaagtgtc tgaaagaatg gctgagcttg tcaaacaagc 28620 aggtggtatg aacgccgtat ctaagcagac tgctacgtca atgggtcaag ctaaggcaat 28680 gtgggataac acacttgtag cactgtttaa caattctagt gagggtattt cacagttatc 28740 taactctgtt gcaatgttct tacaaggttc tatggggtct acagaagctt taggtgttgt 28800 gattggtaac ctgttaaaag gtgctagtaa cctacttgac tttgttacag acttcatgta 28860 cagagtctct gcactatact actatgcaag agcttggtat aaagaccttg acagtagtca 28920 gcaaaaacta gtcaaaagtg ctggtgaatt tctgggaaca gtcactatga ttggtggtgc 28980 tgtagcaacg gtagctaaaa tggcaaagct tatcactgga ttctttgata cagcaattgt 29040 gagaaaaatc cttcagaggc ttggtattga ggttgcagaa aaagcagcac cacgagcagc 29100 accattactt gcatctccag ttggtgttgc agcagcaacg cttgcgctat ctaagtcttc 29160 tgaccctaat gctggaaaga gattcaatga agctaatatc actaatcctt tcaatgaagc 29220 tgttgcaaat attacaaacc caaaaagacc aatgttcttt gatgagaatg gtcaacttaa 29280 gtttgcacaga tacactcaag atatggaagg taacagaaag ctaattgaca atggtctatc 29340 taattgggat attctcatgg agaagttatc agcatctctt gataattttg ccaataagtt 29400 taatcagaca ccaatgatga tgacaccttc tggtttacct atgcagacta aacaaaccct 29460 gaatgttact ttcaaacttg atggtaaaca aattgctact aagatggtgg atattactga 29520 caagaatcaa gaagacattc ttctaagttc aagctatcca gaggaagaat aatgttatgg 29580 gattctaata tgcaaatcaa atatagtggc aaagatggca tctatttcca cttaagagat 29640 aatgtagatg ccttcttaac cttatcagca actgaaaaca tggaatttga cagccctatg 29700 caggtaacta cacagaacat gcaatcaggg caaaccgtca cagataatgt tcaaagagca 29760 cccagaacaa tcactattag tggtgtcgtt gtagttggct atgaaggaag cttattatta 29820 actcgtcagg gtcaattagt agaaaacttc atcgacactc tggaaaactg gcgtgaccag 29880 aagcagatta tttcggttat ctgtaaagac ggaatcaaga ttgatgattc cattattacg 29940 agctttaaag cctctaaaga tgttggtatt tcaaatggtc taagaatcca actaaccttt 30000 caggaaatta acttcaaagc tgttgtgggg caaactgata tctctgctgc aactggaaag 30060 gccgctacta cgaatgatgg tggtgcaact tctaagaaga atactggcaa cactacgaca 30120 agtttaggca atggtaaatt aaactgccag ttattatttg acctaaacgc caatggtgta 30180 agggaactta ctagtgctga agataaggct cttggaaaat gttcaatgtc tgcaaagaca 30240 agaaaaggtg ttaccacatt tagtgacgag gctgaaaaaa atgccggagc agcgttaaat 30300 aggactgctg gaactggaaa ggcattacaa aagcactcag tgaatccgaa taagaaggag 30360 acttattaat gtcacaatat attcctgttc ctgatacaga atggtctaca caaactgtaa 30420 ctctggatgg cactgtcttt gtaattgagc taaagtataa agaaaggctt gacagatggt 30480 tcttgacgct atctgatgtt gatggtaatg tattattaca tgaaaagaaa tgccttgcag 30540 accaatcaat cacaggacgc tatgtaattc cttcattagc tggagagctt tttgttgaac 30600 gaatgtacgg tactgattta caacctacca gaaataactt cggaagagaa aaggcatttg 30660 agcttaatta ttacactcaa gaagatatga gattaatgga gaacttataa tgtctgtaaa 30720 agatagcact gctggggctt cttttaggtg ctatcaattg gctgtaggta gtgaaactac 30780 agcctttaat gataaaccta caagccatgc taaagattct atgcaaatgg actatttcga 30840 caacttacaa ttcacttgta atgtgtctta tacgtctcag aaaaataaag taacctctga 30900 cgataccact tttgaaatct acaaccttaa taaagagatg agagccaaat tcaaaaccgt 30960 tggtgcaaca gttatgctca gagcaggtta taccactggt tttaaaaggg actcaaacgg 31020 tgaccttatt attgaatatg acaacctccc attaatttat ctaggcacta ttgagtatgc 31080 ttatacctat aagcgtggtg ttgatatgat tacgaaggtt atctgttcca atgataaaat 31140 ggaaagaacc acgattaaga catcaatttc ttataaagca gggacaacac gtaaaagtgt 31200 gattagagat ttagttagca gattaggctt ctcacttatt gatgaagacc tttcaagtat 31260 tgatggttac acttacaaga atggttttag tgtttggggg agtgttgcag aggcacttac 31320 aaaggtttgt gaagaaagta gtctacgttg gtatacattt aacaagcaaa tccgagtagt 31380 cccttttaat gctaaagcta gacaactttc ttgggaaatt tacccatata acgttattga 31440 ctctttgcaa ggttactaca gaagaactag aaaggttctg aaaaaagaga ataagacagt 31500 tattaaagtt aagactggag ttcgttgcaa aatccattta gatgggagaa ttaagatggg 31560 tgataacatc actatcagag aaagtgaaga ttttgaaggt cagtatcgag taaaaggtct 31620 ttctcataat cttgacttta ctggtggtgc ttggacaact gaactagatt tagagaaggt 31680 ggaataatga agtcaccagt tactagaatg tctgggtatg tttcagaatg tcttgatgaa 31740 tttagaaaag aactgtatac tggattacca gctattatcc agtcttttga ttcaaagact 31800 caaacagcca ctgttaagcc actttactct attaacggtt tgcctatgcc agagattact 31860 ggtgtacctg ttcaattccc aagtggtggt ggagcatctt taacattccc cgtaaaaact 31920 aatgacagat gctggttagc tttctcaatg ttacctttag atgacttcgt tgtcaatgac 31980 aagaatgttc agatggaaac aaacatgaga aggacgcacg atatttcaga ctgtgtagct 32040 ttcgtaggca tctgcacaag aacacagaat tttaaaccag acccaacagc agtcagactc 32100 cattttggtg actctgtatt aagaatcact gatgatggta atttctactt tgaaggtgat 32160 gtacacattt ctaaaaactt atatgtaaca gaagaagtac atggttcaga ttttatcagt 32220 gacacgactg gtgtgagctt taatgaacac acgcaccatt attactggac agaccctgct 32280 ggtgaggctg atactacaga ggcacaataa tgaaaacaga ctttgcatta aatctaggtg 32340 gtgactatgt tgccacttta ggttcagatt cagtgtatgt ggctcatggt gatttaaaga 32400 ttactggtaa ccaaattaga attatcccag aggatgataa agctactcag gttgctcaaa 32460 gacttcatat tagatgcctt ctaagggctg gtgaagtctt ctttaataca tctgctgggt 32520 tcccatattt acaacttgcc aaatttaaac agaaaacttc tatctttgat aattatatga 32580 aagcctacct tgttgaaaca agagatgtat ctaacatcta taactattct tcttcaatgg 32640 acaatgctca aagaaaagtg actgttaatt ttgatgcaac cactacaaca gatattttaa 32700 cagacattac gcaagaggtt aatatctaat ggcaggacta actacaacag gattacaaac 32760 tctaagatat caggagattt ttgataatat caaatcaaga cttcttagag atatttcacc 32820 aaaccttgac gtttctgaag acagtcaatt aggactcttc ctagcttcaa ttgcaaggtc 32880 tttagcagat actcatgaga ttctatcaga agtctatgac ggtggaacaa ttgacaaggc 32940 cgagggtttt aaccttgacg atattacagc tttgaacgct gtctacagat atgttgctca 33000 ggcaacaaga ggtcaagttg agtttactgg gactactggt gctaccattc cttcaacaac 33060 aagattaaga agtactgccg gaaacatctt ttatccagtt aataacatca cccttactcc 33120 atcatattgt gttgaagcta cacttgaagt taactcttta cgaactgatg cagattatgt 33180 tattattatt gataacgtca ttttctcata taaaccaacc tcattagaca caatcaccac 33240 gctgctaaca aaattagcag aagctattaa tggtggtatt gtagcaaaag cagaagtcat 33300 taatgatgga gccgcattaa gagtctataa agacgaaggt gatatcattg caagaacaaa 33360 ccctatggtt gtcactgcaa caacgtttct gacctttaca aagattacaa ctatttcaga 33420 tgtagtagct gaagaggtgg gtgctacccc tgctttggct ggtacgctta ttgagattga 33480 gacaactgtt gatggtcttg acagtattta taacagatat gacttaacaa ctggtagaaa 33540 cgaagaaact gacacagaac ttagacagag atatttagag tccttagcag ttactggtgt 33600 gggtactttg gatgcaattg tagctgctgt taaaagagtt caaggtgtct cagatgcttc 33660 aggtgttgaa aatgatactg aagagacaaa ctcagaaggt cttccaccaa aatcttttaa 33720 gattgtcgta gttggtggtc aaaatgataa tgttgctcag gcaatctggg atacaaaacc 33780 tgctggtatt agggcttatg gctcaatttt tggtacagcc catgatttag gtggtttggc 33840 acataatatt tattttagca gaccaacacc aaaatacgca tttgttaagg tgtcttactc 33900 actatacgac gaagaaaact taacaatccc tgaagaggat atcagagaca gcattgtaca 33960 aggtattaat gcctacggaa gaactcttaa ggttggtaat gacgttatcc ctaacagaat 34020 ctatggacat atctatgacg taattaaagg tattgagatt aatgaagtta aagtggcatt 34080 atcaaataac caatcagttc cacctagtga cgggcagtat actacagcaa gaattactgt 34140 tgacggtgac caatatactg tttgggaaag tagccagtat accatcacta aggagtaata 34200 atgtttgaaa agattgatag tgtttattat aaaacacttg atgaaaggac tgtaacacag 34260 tttaaagata aatttatcta tacaagtctc ttgaaagcaa tcactgatga gctacaaaca 34320 ctagaggatg tttgctggca gatgcacaca gagagaaata tcaggacgtc agtaggtcaa 34380 caacttgata atattggctc actgattaaa gttcctagac ctttaggtgc tgatgatgaa 34440 acatacagag ctatgcttta tatccaaatc ttcctaagac gttctgatac tactccaaca 34500 ttcttgcaaa aagcaattat gacattgtat ggtgcaactt tctctcagat ttttgagcat 34560 attactccta agactggtgg tatcgttatc cgtattaata ctagaaaaag cgttcttgat 34620 gctgcatata cactgtcaaa gatttctgca acaaccactg gttccgctgt tatcttaaga 34680 gatgttactt taaatgggac tgcttggaca cctgtagagg ttgctgattc atcacttgct 34740 attgttgatg ataaagagaa ctggtttgta actgatgcta acaggggtct ggtaacaaat 34800 aacactggtg gctctctgga gtccaaccta gtagggagtc ttgcagatgc tggtgttatt 34860 gatggttact ttaaggttga cagaacagca aacccacaga gcgatgatta tatgactgta 34920 gataggtcca ataatagtgg ctatttcatt gctggtaaag aagctattgc aggtggtgca 34980 tatggaatca tggctgaggt tgcacaaatt gtcaaaggca gaaaagataa atcacaacaa 35040 gaaggaaatt cttaatggca gttctaaatt ggtctacaaa cgaagtagat actgatggta 35100 atcagttaaa agttttacca ccaccggaag ttcaagagac aggtctttta aagggtgagc 35160 ctatgggtag acagtggttc aactatatta tcaactacct gctgaataaa gtaaatggca 35220 ctgttggtga agttcgttca tatgcaactg agcaaccaga tttagtggct aatggctgga 35280 atctcattaa gtctgagaca gggactgcat caacaagtac aagaaacctt tacacatatg 35340 aatatgtagg agcttaataa tggcagtagg tgaaattcaa attagtgcct tgccacaagc 35400 ggccttacca gttgatttga gtgatatctt ccaccttaag caaggtgttg aggataaaag 35460 atgtacgctt gagcagttgt tagcacctca cgcatctttg aggaataacc ctcatggtgt 35520 aactaaatca caagtaggtc ttgataatgt tattaactct cttcaattag ttgcagctaa 35580 taacttgtca gatattgtaa gtgttgctga ggcaagagca aatctacaaa ttatgtcgtc 35640 agaggaagtg aataacttaa ttcagcaaca tattgacgac aaaagtaacc cacacaatac 35700 aacgaaagca caggttggtt taagtaatgt tcagaactgg acaacatcta atctgtacaa 35760 tgaagatgct gataagtatg ctaccgcgaa agcagttaat aatttataca aagctatcca 35820 agcgtcatat ccagtaggga ctattcatct atctgttaac tctgctaacc catccactta 35880 cttgctttgt ggaggtactt gggagttagt ttctaaaggt agagcactag ttggttatga 35940 tacagattct agacctgttg gttcaacgtt tggttctcag actgtagcat taacaaataa 36000 taatttacca gcacatactc actctatcta tctaacaggt ggtggtcata cacactctgc 36060 aagtgtatct atctctagtt ttgattatgg ttcaaaaagt acatcaactt ttgattacgg 36120 tacaaaaacg actaatagtg ctggtgctca cactcacacg tttagtggca caacaagtaa 36180 tgctggtaac cacaaccata gagtccctat gaggggtaat gataggggtg gcacaaacgc 36240 tatcactgca tctgctgatg ctggtgttgg taatgctatg tatactgatt tagctggtgc 36300 tcacacccat agcttctcag gtacaactgc atcatctggt gctcatagcc atactgtcgc 36360 aattggtgct cacagccaca ctgttaatat tggttcgcac agtcatacag gcactgttac 36420 tgtttctagc tctgagcata cacactcagg gacatctggt tctgttggtg gtggtcaagc 36480 tttcagtgtt gagcagccat cttttgtagt gtatgtgtgg cagagaacag cttaatttct 36540 ttacaggggc tataaagccc cttttaagag gaaaattcat aatggcagat tacaaattga 36600 gtcaattaaa ctcaatcgac acaattcgtt cagaagacct attgcacatc agagttaaga 36660 aaagacctga aatgttgggt gacgaagacc gtagaatgac ctatcaagac ttcttagctt 36720 ccttcaaact agaaaggttt gttcagattg ctgggagtac tatggttggg gatttaggta 36780 ttgtcaagtt actttacggt ggtaaggcag tatttgaccc aacaggctct tctgagatta 36840 ctattgggga tattttaaag acttttaaaa ttaactcaaa tgggcttaaa ctcactattg 36900 cagatgccac aaggtcggca actgtttatc atactttgaa taaaccaagc cctaatgaac 36960 ttggtatgag aactaatgaa gagaatgatg caagatatgc aaggcttgct gttacaaaca 37020 cattctctgg aactcagaac attcaaggtg atgctaactt acttcgtctt agaaaccaga 37080 atgcaaataa tgcgcaatat attgagggtg tgaacttaga tggttcagct agatggtggg 37140 ttggtattgg tagtaatggc tctgatgaag taaaactgta caataacaaa tacaactcag 37200 ctttgactat tgcaagcaat atctctgtta ataagtcttt agcaatcact ggacaagttc 37260 aaccttcaga tttctctaac ttagatgcta gatacttcac tcagacagtt gctaatcaga 37320 gatttgcaca gttagctggc aataatactt ttacaggtgc taacacattt aggactatga 37380 gtgttatttc aaatgctgct gctatcacac tgcaatgtgc atcagcaaac gaatccctgt 37440 atctacttgc aaaagattat gaaggtggaa acttatggta tcttggtaaa ggtagttcag 37500 gtgctggtgt aactatctac aactatacca ctgacacctc tttggtctta gatgctgctg 37560 gagtatcagc aaataaaaat gtcagaatca ctggacaagt tcaaccttca gattgggcta 37620 acattgactc tagatatatt ccggcagcaa cattaagtac aattgcaaga actaatgcaa 37680 ggaatacttt taatggtgta cagacagttg ttacggataa tgaaggtctg attgttaaaa 37740 actctactca gaatagacca ttatatattc gtggtgtgga cactactaat gtatcaagat 37800 ggtggattgg tgttggtggt gctgatacga atgatgttac cctaaataac agttattctg 37860 gaacccaatt ggttctcgga aatacaacat catacattaa caaaacattg actattgctg 37920 gacaagttca accttcagat ttctctaact tagatgctag atactttacg caaagtgcta 37980 gtgatagcag atacctaaga atcagaagta ctagttttaa tttaggaaac ggtgaaaagt 38040 gggctaaaat cgctactgtt gtaatgccac aatcaacatc tacggcagtc attgaggttt 38100 ttggaggtgc tggttttaat gcgaataacc cataccaagc aggtaagtgt gaaattgttt 38160 tacgaacgtc aaataataat acaaaggatt taaatgctgt agcttggaga acagcagata 38220 atgccctcat cacggatatt ggttatatta acacttctgg tgacacttat gatatttact 38280 gtaaggttgg tggctaccaa aattctacgg tatctagggt tcaatcttct agtaatgcaa 38340 gtgtacagtt gtttgaagtc ccgcaaacat ttgatgatgc tccacaaggt attgtgaagg 38400 gtacacttgc aaggtactac acaagtttgc aaaaaccaac cccttcagat attggtgcat 38460 acactaaagc agagactgac cagaaaattg cagaagcgat tagtgactct acagacctga 38520 ataaaatcta tccagtaggt attgtgacgt ggtttaacag caatgttaac cctaatacag 38580 cacttcctgg gttaacttgg acgtacctga acaatggtgt tggcagaact attagaattg 38640 cagcagcaaa tggttcagat gttgctacaa ctggtggttc agattctgta acgttatctg 38700 ttggtaactt accttcacac acgcacagtt tctctgctac cacttcgacg tttgattatg 38760 gcactaagca cacagatggt cagggtgctc acgaccatga tagaggtaat atggaaattc 38820 atggtgattt tggtttcttc aggagtgaca catcaagctt ctacactgct agtgggtctt 38880 ttgctatctc aggttcacaa gcatctagag gatacacagg taataacttc acttacggta 38940 ctcctgttaa tttctatgcc tctagaactt ggacaggtag aacaagttgg gtaggtgctc 39000 atacacatgg tgtaggtatt ggtgctcata accatacggt aagtggtaac actggtggta 39060 caggttctgg ttcagcattt agtgttacta accagttcta caagttaatg gcttgggtaa 39120 gaactgctta atcccttatt gactgattgt taagatggtg ttaatattct ttatgggtat 39180 tctcaccatc ttggctgatg aggtgattaa atgcctacaa tcctagcaat tcttctaaaa 39240 aatctaggta gcttcttctg gaaactcatt ttatcccttc taagtgaata catgattgag 39300 aaagtgttct ttaggcttgc aagatacctt gcgagtaaaa cagacacacc tattgatgat 39360 gagttcgtag ataacttaga gaaagctttt aagggggaga ataaatgaag tggctagagg 39420 aagttttaa aaataatatt ggtgcaattg tagttggtat ttttagtgtt attgggatgt 39480 ataccacgat gcaagtttca agtggtaaac aagaagtgtc tgtctcaaca aagttacagc 39540 agctagataa ttattcaaaa agtaattact cagctattcg tgatttacaga tctgacatga 39600 gactgctaca gctagggatg gagaaccaga aagtccagtt agagaatgtt aagggtgaga 39660 acgcaaaact tactaagact cttgataaat tctctgatag tgtgaacaat ctggctcaat 39720 cagtatcagc cctccaagct attactgaaa agaacacaaa gaatactgaa aagtaaagtt 39780 taaggctcct ttttacggga gccttttttg ttttatttac ctactgacaa aggtgcttca 39840 atctttcctg catgttggta attgttaata ccaccaataa agtcacttgc agtgagatgt 39900 tttaagtcac ttaaagtgtt aagtgggata ccaatctcaa aagttggtgc atggaactct 39960 tcattgttca tcagttcata aacctgtttc atatggttct gataaatctg agtatctcca 40020 aagacaccta tcaagtaccg tggagtatat ccagtcatct tacaaagaac ttccagaata 40080 aaaccgtaag atgcaatatt aaatggaagt cctaaaaagg tgtctactga acgctgatac 40140 cattgtaagt caacttcacc ctcgttagta atataaatct gaaacagaac atgacaaggt 40200 gctagagcca ttgagtttgc tgcaatatct gccgcattcc aagcattaac aagcatgtaa 40260 cggtctgtga tatcaccctt catcttcgtt actaaggtct ctaactggtc tacaacacaa 40320 ccgttatgac cttcaaagtt tctccactga acaccataaa ttcttccacc cgcatcttct 40380 aaccaatctt gttcagaaga gtaattagag cttaaccagc gtttaaaatc atctgaccag 40440 atagtccagc gttctccatc attctcaccc caagtacggt aacggagttc acccagctta 40500 ttctcaccat tcaggaacca taaagcttcc ccgataactt gccgtgtaaa cacctgttta 40560 gatgttaaga gcgggaaacc agttcgcata tcaaaacgaa actgaggtgg agcaaatgca 40620 gagataacat ctccagttcg tgtagtacgc agttcaccaa ctgataaaac atggttcagg 40680 atatttttgt aactcaaatc tgcttgtgac atatcaaaag tgtcctttta cttgtggaac 40740 ataaatttca aaagttgctt taccatcctc tgatggtttt gactgtacct ttgtaaatac 40800 acggttatca tacagcttct caaagaagtt ttcaaaaggt aaatgcacaga tggcctcttc 40860 agtaacttta tggaaaactg tatgaaacac tacatcagca tacggtaaag cattgacaag 40920 gacacctgca ccaccaatta caaagacatc ttcatcaata gagctatcaa gatacttcaa 40980 aaatgctcca aatgactctt tactggcttt agcatacatt acctcatctt taccaaagtc 41040 tatgcctaag tatggtacag agtttgttaa gattaggttt gcacgttctg gtaaaggttt 41100 actacccaga gtcttaaaag tctcattccc catcactact aagttatttt tagtgagtct 41160 cttaaactct tgcatatcct gtttatgtcg aggccacggc ataccagttg gagtaccaaa 41220 ctcaccattt tcaccacttg caaagattaa tttaatcatt ttgtaaaccc tttcttaatg 41280 aaccagttga taacattctt cacagcttta ttgctatggt agattaggtg tggtgtaggc 41340 tggtatgaac cataggccca gaatgataga tgctcaccac aaccaacaca tgtagtactt 41400 tcttcagcta tatgaccatt aacaatcaca tgaacttcac cttgcaagtc tgtacaccca 41460 caatgtgggc aacggatagg tgttccatca gactcatagc agtgttcaat tttcatttat 41520 ttaacctcaa aaactggctc aagaaccaat ataaagtcta cacaatcatc ttcttcatcc 41580 agaataccat caaagttctt aggaacgaag tcgaagaggc tcataacaaa acagtcagaa 41640 gtaaccctga tagttcctcc catgttctct acagtcaaca ctgtacgact ttcttttacc 41700 tctctcagaa gattactcac aaagtcatct aagaagtact tcatcagtga cttaaaacct 41760 ttagggtaat ctgtaccaga gtatgtttca atctggggta caagcttaac atttatacta 41820 gtctttgtta agaacgtgct agagaacatg ctatcaggta acttacaaac aatctttcca 41880 gtaccttcat gctcaatctc aacaaaacca gcttcgtcgc cacatgtaac tattttaaaa 41940 gatgcttcat tctcatgctg tgaagccata aactttttaa gcatcacttt aaggaaatct 42000 gcatgagcat cttgtaagta gatatcaatc atagttaatc acctattatg ggaacagaca 42060 cttgatgtca tcatttttga aaacaaaact tgtgtagttg tgaccatcaa catccagcca 42120 aacagcctga acatctacac catcaagatt gtggtaaagc tctttgatga agtggtcttt 42180 cacaacctga ctactagttg tttccagctt aatgtttgag attgctgaac ctacagccat 42240 ataacccatg acacgtttct tctcagtatg cttaatctta ccatctttgt aagccataat 42300 gagattggaa agaatcttca caccataata cttttcaagg tgagtaggta caccgtaaaa 42360 acctaatgcg aagttttcag agtggataat gttttcacgt ttcatattaa tctgccaacc 42420 ttaaattaaa attaacacca aatttttcac atacttccaa cataaactca agagagacat 42480 taccagtgag gttgattatg ttggatacac gagcttgaga gataccacaa atcttagcaa 42540 cttgctcttg agaaagacct ctggatttaa tctctctctt aaaacgatgt gcaacgaagt 42600 ctctcatttc gtctacatcc attggacaaa tataactctc catttcagct tctctgtcag 42660 cctcccaatc atcttgtgga gcataagggt caaaaacctc attcatcttt cttcttccgt 42720 ttacgtgaag catcaatcaa tgaatctatg tttgtagctc taaccttttc acgttcatcg 42780 ttaagataat catcaagagc cttctcaatc tgtgaatcat cttgacagat aactgtaaag 42840 actgtaaagc catctatatc atcaattatc ttaccataac cgcttctggg atgtaacttt 42900 ttaagagcca tatcaaatac ctgtgcagct tctgttttct aagaaatact caacaccata 42960 catagctttt tgcatcaagt cttctttaaa ctgaatacga tgattgagca tagcctgtac 43020 atcaatatct tcatggtgct tctctttaat gtttgcagat tgcaggaaat actctgcaag 43080 aacacgatac tgatagtcag ttaccttatc agtcatctct gttaaagact cttcagagat 43140 tggtttaagt aactcaccat tagccaaagc aacaccttta acaatctgac aagtgtcttc 43200 tggaacacct gataagtcca catttgggag ttctgttgca ttgactggta aattcattga 43260 cataaccatt gcagcagcta ataaaatctt tttcataatg acctcaaagt tatattaaat 43320 tttgtattac attgagtata aaaataagct ataacacctg atttgtcaac agattttata 43380 gcttatttgg cagatttttt ttataaggct actgccatcc ttccatacca aaccatacca 43440 gacatagcca tacccgaccg tacctcgcct cacaacactt aaaaatcctc ttagaagact 43500 cttaagtgat gtaatcgaat tttaatgtag ctccgattaa ctacccatac ctcacctcat 43560 ccaaccatac catgccttac cagaccgcac cttaccttat taaaagaccc tatttaaaga 43620 ccttttaaaa aggtgagtct cattttaaat gtagctgaga ctaactaccc atactgtacc 43680 gcacctgacc cgacctgacc agacctaaca ttaccgtgcc acaccgtacc gaacagaaca 43740 ttgccatacc tcgcatcact taaaaaccct tttaaaagac tcttaagtga tgctggtgag 43800 tacccgtcgg tttatgctgc tcagtatact caccaaattt tctatgtcaa caacttttat 43860 tcataatccc taataactag tccgacaggg aactgtaaag aacccttacg agtcatcttc 43920 tggaactgta ccgttaaagg cttcccgata aactcttcag gatgctcagc aagatactgt 43980 ttcttctcat gagtagtctt ccatgagaca tctacaaaga cgttaggaag agtctctaca 44040 cctatcagtc tgcatcttct tgtgcttaat caagaagtat gaacggtgac aacactcata gaaggagtct 44160 tcagagattg aacggtacat tgcaccttca aacttagctt caacccactt atcatgagct 44220 tcatcaaact cttcccaaga atttacacga cgagacttaa caggaacaac tttacagccg 44280 tctctgaagt cattcaatgg tgaagtctca ataatatctc tacgctcagg ccacgtctta 44340 gtactatcac agatatcgta ccagtaaaac ttgagcaagt gtctgtcagg attgtcttca 44400 tttttaatca tggagacgat atcttctaag tcccacccat gagcataaat ctctccatca 44460 aattcatcaa catctttgtg gagccttagc attgccataa tgtcaggtat tagttcggca 44520 gggattttat aaacagtatt ttcacgagaa tatgctgtaa atacactgcc aatacttaag 44580 tgtcttgaaa ttctacaacg gacaccatca agttttggtt gagcatcagc agggaacttc 44640 agatactttg catgactaac ctttgcagca tcatgagcaa gctgcacacc taccttctca 44700 gtattctgtg cagactcttt tgtgtaagca tagcctttgc ggtcaacctg ctttttatac 44760 ttagcagcaa cttcaaagag agcttgctgt tcagcgttac gctcattctt tttaccgatg 44820 tttttaggtt ctgctgtata ctcttcaaac atcatcttgc cattttcttt gccatacgtg 44880 gtaacaactt tgtcacctac agcgacacat gaccagacgt taaaagagcc atctttattt 44940 tgtttgtaca gtattgtcat tttattcctc tgagtgatac caagtattgc aagcattaca 45000 tgcgtaaagc tctaagtcat ttttatctgg tttatgaaaa cagtttgctc cgcagtggca 45060 acgaaacggc tttccaccaa ctgtcaggat gaaggattga acctttccat caggagtccg 45120 tacagcattt ttatgcttgt cttctttatt catttgtgct attgcctttg tcagatttca 45180 tataggacat gttactatgc cttttaataa aatgtacaac gcattttggt gtatcttttg 45240 tgtctttgat gtgcaatcta tcgaaactgt gtactgtgaa ggttgcatct gtcattgtct 45300 gcttaggttt cattttcttt taccaccaat ctctacgtca ataacttgaa catcaccagc 45360 aacatcaaac ttatgaatga tgctttccac agtgtaagtc caaccctcaa tgcgaaccaa 45420 ctcaccttct cgtgggacga ttgggttacg ttgagctacg gttgtagtgg agtgaacaac 45480 atcataacag tagatatagt ttactaggac ttccatatct tcaccttaaa agaataagtc 45540 agatgctttt gggttgatta aactcatgca gttttgaagc atggcttcat tgtacccatc 45600 ttggcaaact acgtagagtt tatcgaaagt ttcttttgat gctgggtaga ctgataactc 45660 ctggcataca ctaagatacc attagtaatc taagcctctg ctccaagcgt cagcgattag 45720 ggctgtagct ttgtctttgc ttctgataac ttcatcagac ttttcaattt tactgttcac 45780 gatgttctac ctctctgtac gttcgtcgat agtatatgat aagctgtgct aactcgttgc 45840 ttttaacatc tgaacgtagc agttgccaat caaaaccttt accgactctt ccatataagc 45900 taaataattt tactttgcgt ttcattttgg tactcccaat actcagaggc caatactaat 45960 acgttcaccc ttgaggactc ctgtacggtc tcttgcaatc ttgccgtcaa caataaggat 46020 atgcgcagac cttaaagatt cactattaaa accttttacg aacttgctag gcatcactac 46080 aatgtacatc ttctgaatca actctggagt gtagcagtta tcatgaagtt gctgaataac 46140 gtctgcaagt gtcatttttg atgtctccaa tattcaatat ctacaagttc ttgtgcaatg 46200 cttttcagat tacctttcaa gaaaaccttc tcagaacctg aaagagatga attgtcaatt 46260 aacctgttca tcctctcaaa caacttctgt ttacggttct tgatagtttc ctttgaagca 46320 cctttcatca tcgctgcatg ggttcttcca cggtggctca taagccctcc tatataagat 46380 attgtataag gtactcttcc ctgagcacct tgtcaactta gttttgcaaa ctctccgtgg 46440 agtttcacag cagcttcaca gtaagcttta tgggcttctt caggtgtgtc atggtgacct 46500 aaaaacatct tcttaccttt ataacttatc tgtgcgaccc acttaccaga cttcttctta 46560 aggcttacac ccttgtaccc agatttattg gtggctagtt tacctttatt ccaagcactt 46620 tggaagtcat ctgcttccct taagtttgca atcctgttgt caagagagtc tccgttgata 46680 tggtcaatga taccttttgg catcttgcca tatacataaa gccatgcgag tctgtgtgct 46740 ttatgtgctt tcttgtcaaa atagatacta atctgtttat gaccagttga atcagtctgg 46800 atattacctg ccacctttcc tgcataatta ctgttaaaaa cactaacagc tcttgattta 46860 ccttctctac gaagccaagt aaagacccct gtttcggggt catagtgcag cagctccttt 46920 aaacgttttt gtaaaattaa atccattaat caatgtacct ccaaccagct cttaccaacc 46980 tttgctgtac cagttacaag tgtttctttc ctcaaaccta agttttttga agcttgtcca 47040 tacatccagt cagcaattgc tttcatatcc tgtgtcatac cctctggaca ttcccaacta 47100 ttttcatcgt gatatgccag aagtaacctt gctccgatag ctggttttcg agcattcagt 47160 tttgtcaggc catcttcaca ggcttttctt gctgctaggt tgatagcctc attctgaatc 47220 tgtgcttccg aactcatcag gaggtagttc agtagtttat gtggagattt gcaccatatc 47280 catgctccag cgaccctgat ataaccacct ttagcaattg aagaattctt cccaaaaacc 47340 tcttccagag cctttttaga cgccttaaaa tcggcttcta agctatcaag taacttctta 47400 atctttggca gacgcatata gtaagtttgt ttagtcaatg caccctcttc cgtagatgat 47460 gccttgatag tttttgcaaa cttctcgtct ccggctccaa ataacagggc gtagatgcca 47520 tttttgctct tcttacgacc ttttgtaatc tcgtgaagaa gttcctcatc ctgtgtctcc 47580 ctgcaacgaa caatatcctc ttctttgtta agaccaaagt aaatactgtt cagtgtatgc 47640 gcatcagtcc ctgtgtagac ttcatagagg tcattttcag catcataacg cagatacttg 47700 tcagtttctg ggttaagata cttgtcaaga tgtttacagt agtatctacc atcatcttgc 47760 ttagtgaact ccactgactc tttaccctca gttaccgctt tagtgaagtc ttcatcaccc 47820 ataaagttac aaaggagaac tagctgggct gagttttggt caacagaaac aatgttagtc 47880 ccctcttcac aaatccacac tgccctcata ggtgctccgt agacagcagc accagacggt 47940 acgtttacaa taccatactg tgtcatacgt ccagttgaag taccaaacac cattgcacca 48000 gcactaaggc gaccatcagg acgaatctgg ttcaaccaac ctttttcatc atcctttgag 48060 ttctcaatag ttctgcgtcg gtgcatcaaa gtatagtact ttgcaatctt ctgtccaagt 48120 tcaccttcaa tcgtatcata agatgactca gtaagctttg gtgaagtacg gattaagcaa 48180 ggctcaagca aatctgtgta cttcttcaca gaccagttat gctcaatgta ctgtatacct 48240 tcatgttcaa cataactcaa accacaccgc tcaaccattt cctgccactt aggatgtttt 48300 gtaatcatct ttttattgtc tttgaaacga caaactttga cagggcgacc gtctgagtct 48360 ttcttgtagt tccagtcatc tggaatccaa ccaactgatt tcaagtagtc tttaacaacg 48420 gctacctgag tcatacgaga aacttcaaac tcaattggtg tatatggtgc atcaatcaaa 48480 cctgtgtagc gacttgactc aagttcaaag tggttaacaa cgtgactgtt atagtacttc 48540 acagtcttga ctgtttttac tggtttccag tctttgcatt tcttaccaat ctttgcgttc 48600 aactcattgc aaattgcacg agcatccttc attgctacaa aaccttcctt gtactcttca 48660 ccagttacag tgttggttgg tgtatagcaa tttctctttt caatgttaaa aatctttgta 48720 gttggcttac caaatggttt aatttcgtat gtctgcatct caccattacg tacctgctga 48780 cgatactttg tcttagggta cttggtaatt ctcttcagtc catctgcatg accaaatgtt 48840 tcaacatact cattccaagc ttttgcaaac tcttctccag tgactttacc tttggtctta 48900 atagttggag gcaggtgtgg ttcaacctct gaagcaagct cattagtcaa cttgtcaagt 48960 tcctttacat ggaactccat aagctcttta tcagctttcc agccattgat agcttgttga 49020 ctcatccaga aagatgtttc tttagctcgc atataggttt cataagtgtc tatgccacac 49080 ttctttagct tcagatattc attatccagt gcgcgtttag ttttggcgtt aatacggata 49140 tcttctacaa cacgagtgaa gatttctgca ttccacacac cccaatgttc aatctctggt 49200 ttacggacac caacacgagc accccatgca gccaaaccat gagcaccttt ataacccttt 49260 ggagttggtc tatccatcca ctgaacacga gactggataa gagaatcctg aaagaaatta 49320 ctccacggct tgcactttgg attatcaaag ttccataaat caggtgcaat gtgattgaaa 49380 acccaccagt cataacccag accattatgg atgcaaagtc gttttgcctt taacgcaaac 49440 tcaatacctt cacgtaagcc acccttgatg tacttggtat acttgtgacc gaggataggt 49500 tcatctgtaa agacccatac aggtggctct tcatcgtcag atttatagtc tgcgaaagcc 49560 attacgtgca ctttagtgaa ctcaaggagt aaaccatcag tttctgtatc accaaccaaa 49620 tgtaagtttt taaaatctac gttttccatt tttactccct ctaaaatcat taacctcata 49680 cattactgca aaaaattata aaaagcaagt tgacaatgca cttgacaagg tgttaatctt 49740 tgcgaagtgg gtttttactt aaaaggttac ttaacagttt aaatatacag atactttaaa 49800 actcttaaaa gattatttaa tagcttttaa aaagctttaa agtataacgt atagaatacg 49860 ttaagataaa agattaaaac ttaatagtta cttaaaagag gcttacatgc agaaaatgtt 49920 tgttcaccca gacatagctg actttgttag agctttgcag aagttggaag aacttgacat 49980 tgctgctcaa cgggaatatg ctcaccatca caggagaatg gttgatatac aacatgaaat 50040 agagttgtct gaaaactatg aagatgactt aaaatgctct gtatttgagc atatgaaaga 50100 tgttgctaca gcgagacgta aagctaaaga cacagttgct ttactagatt ctcttaaaaa 50160 gaggttgcaa agtggtacag acctgtgtaa tctagcatca ctgattaacg atgttgaaac 50220 ttcttgggat aggcactatt atccacgttc tgaaaagaca cttgactttt cttcatccga 50280 aaacttaaaa tgttctagaa agaaactcaa ccaactgaga gagaaataat atgaatatca 50340 atgtagaaaa gatgcttgaa gaaaaaggta ttgacgaagc gttcttaatc aatgccgtag 50400 aaagcatggc tatgattctt cgtggcaaca actacacaga acagtttatg cctgcaagct 50460 ataacttgcc taaagataag tctgataacg gtgcttttgc agaacaggtt aatatgatgc 50520 tgtgtgataa aacacttgct ttagcatcta tcgttgtagg tgttgatgct cttgcagagt 50580 tcatttttta tgagatgcag aaaaatgact atattgccat gacctctgaa gaaaaagact 50640 cgctactgct cttaggttat gatgagctag ttaacctgtt aatttcgtca acgagtgctg 50700 ttatggctgc tcttgagaag cgaatggaag agaagctgtc acgatatgaa aagcttgatt 50760 ccaggaatca tggaagaagc tgtgaaaaat gattctgtag ctgacataga gccacttcag gtgaaagttg 50880 cattgatgat tccagccatc tccacgatga tgaatatcgt tgcactaatc cagctttcac 50940 agatgatggg cacaagcatt gagttcattg agagtatgac tggtggtgtg agtatgcaag 51000 caatcaatga tgttctggtg agcattggtg cttcaatcat tgaagagaag ctgcgcatta 51060 actttggtga tgattttgtg aagcaaatct cagctatggc tgaaaaagag tattcacagg 51120 ctaatactga ccaactgcac taagtcttta taaattttac aagcctcctt agttgggggc 51180 tttttagtaa ttaagggaga taaaatgagt aatattatcg catttactgg aaaggcacgt 51240 tctggaaaag acacctcatg ttctattgtg aagaatatct tagaggatga gtacggctac 51300 aatgttgcag taatggccta tgcagacaac cttaaactct ctgcttcaaa gatatttgac 51360 ctgacttgga atgacctgta cggtgaaact aaagagactc cacaggtttt tgatttatca 51420 tactctgaac ttatgcctaa agttacggag gctatcgagt tcaccttcag agatgaacgt 51480 tatcatatgg actttaagct tatgtcagag ttaaccggac gattagtcat ggagcttaag 51540 aaggtcgcta aaccgactct attgacacgt ctagggttta gtaaaaagta taaattctca 51600 tcaaggcaaa ttcaacagat ttggggtact gaagtcatcc gtaaagttat gggtgacaaa 51660 ttctgggcta aagaccttga aaaacgaatg gctcgcttct ttgaatcttg ttcacttatg 51720 aatcaagagg gtgttgtttt aatcagtgac ttaaggtttg actctgaagc ggagtggctg 51780 agtagatttg cacaccagac tattgaagta aaaagagaca atgtggataa ggtttcatca 51840 catgtttcag aaaatgggat ttctaccaaa tatgcgcgtg acattatcca taataatggt 51900 actcttgcag accttgaaag taagctaaga gctatcctta aaatttaaaa gagagcatga 51960 agatgagagt aaaagataat ttcaaagtta ttgaccatcg tttagtggaa ctctcatctc 52020 tttccaatga ggtaatgatt gaacgtctta agagagttga atcacgaagg aaagagattg 52080 cagatgaaat ccatgaactg gacaagattg aaaatggatt gaaggcagaa ctacaacgaa 52140 gaggtgctaa tgtctaaagg tcgtaaattg aaagaggctg gtcagtttgt tggtcattgt 52200 gcatgtccac gttgtggttc atcagatgct ggttcaatct accaccatga cgatgattct 52260 tattcgatga cttgctttag ctgtaacaaa ggtttcccag agtgggattt tgataaagga 52320 cagattgtga gcacttattc tactggttc gataataaaa accgtacttt ccgtgggatg 52380 gatttagacg atgtaaaaga aaaccttgaa gcaatggact tgaaagacag gaagattcct 52440 gcaaaagttc ttgaacgctt aggtatcaag gtcgacattg acagtgatgg tgaaattgat 52500 gcacatttct atccgactta caaacgtaat gaagatggca agctagaaca tgttggctac 52560 cgtgttcgtc accgttaccc agaagaccat ccaaaagaac acctacgtgg taagttaaaa 52620 gacttttcag gtggtgttgg agacattaaa ggtgaactgg caatgttcgg ttcatggatt 52680 gctccagaag gtggcaaccg tctattcatc tgggaaggtg agatggagtg tgctacagca 52740 atctacatga cttctctggc aattaaagac aagtctcgtc gtaagaacta ctgtcacgta 52800 tctgttccat caggtgcaaa cattaagtct atcaaagaca actatcagta cattacatca 52860 tttgatgaga tttacttgtg ctttgataac gatgaagcag gtgctaaagc aacaaaagag 52920 gctgctggca tcctacctat tgagaaggtt cgtttattcc agtatccaga aggtgtaaaa 52980 gaccttaatg aatggtggac aaagttctat aaagagaaag acacagttct ggaaggattt 53040 aagcagcgta tctacaatgc acctcgttat tgccctgctg gtatcaagaa ctttgcagat 53100 ggttttgagg caatgaagaa tcgtggtcag attccattga ttcctttccc agaatctttc 53160 ggggatttga acagactaac ttacggtggt tatggtttag gagagattac aactctggca 53220 gcaccatctt cagtaggtaa atcagcttac actcgtgaga tgatttattc agcttggaag 53280 gaaaccgatt acaatattgg tgtaattccg gtagaagata cttacgaaga acttatggaa 53340 atgctctgtg caatccacct gagcaagcag atttctgaga ttccttatga tgaacgtgat 53400 tgggatgaac taaagagagc acacgcagaa ctatctaaag gtcgtcgtat ccatatcgtt 53460 gaccatcaag gggcaattga ccaagataac ctgctggaat ttgttgacta tcttgttaat 53520 agtttagact gtaagattat cattcttgac cctattacgt tggctctgtc acgttcagat 53580 actgatgaag aggaagtttt gtctgagcta ttgcgtcgtt gcaaacgcta ccagtatgca 53640 caggtaaacg tctgtcacgt tcgtaagagt gcaggtggtc agaaggctaa ctctgaaggt 53700 ggggatatct ctgaagagga tattaaaggt tctggtgcgt atttccagat ttctatgaac 53760 aacatcctgt taatgcgtaa caaggttgac ccagaccctg ttaagaaaaa cttgacaaaa 53820 atcaagctaa ctaaatgtcg tcggcatggt aagtcaacgg gtattgctgg tcatacttgg 53880 tacaatccag atacaggacg cctcatcaaa gcatctggtt gtggagttga cattgatggt 53940 gcagcagaaa atattcgcca acagtttggt attggtgaag tggaagacca ttacgatgac 54000 tttttgccac attatgagga tgaagtgttt gaccgtgaga ctggtgaagt ctatactgaa 54060 gagcagcgtc aaagctcaac aattccacct gtattaagtg aggatgcaga tgactgccca 54120 ttcgaaactg agtgatagtt ataaaagaga ggggttcata cccttctttg aagaaaaaag 54180 tttaaataaa tttcaggaag agtgtttgac aaagattgat aggttctata gaatgctcct 54240 catgaaatgt gatggggagg ttgctataaa agaagattcc taccaaagca cgattacagt 54300 catcattaca ctgcctcagc ataatattag ttggctgttt ataattaaag aaaatgtgtt 54360 tgaatatcaa gtatatcgga gaataccatg aaacactcta aagcatttga aaaagttttt 54420 ggggattcct taaaagccac tgctggaaaa ccagcaaaat actatgaaga aaaacgtgta 54480 agaactggaa aaactgcacg taaagcagct tctaaagata agcacaactt ccagtaatta 54540 gtattggaca atagagtatc aacaaactag aattggtact cttcataaat tgagatagag 54600 gtttaaataa tgtctaaagt tgttaaaatg aaagctccgg tagagaaata caatggtact 54660 gaacgccaaa ctctgcgata cctgctgaaa gatgtctggt tttattacct gaacacttca 54720 ccacgtccgg gaaaaggtaa gtctattgat aagaaattcc caggcaaaga ctgtaactac 54780 agcgtatcaa ttctggcaga agacggtaac aagctgttta aagaatttac taagtctaag 54840 aaaaacccag aaggttggga taaagttact actgaagcag ttgatgcaga tgacttcgaa 54900 gagaagtttg gttgcaaacc accatttgaa gcagatactt actacatctt gaaagtaagt 54960 cgtgcagcag cttataaaga tggtgctgta tggacagcta aacagtcatt ccctgtaatg 55020 ctgattgaag aagtaaatgg taagcgtgta gctgttaagc agccgatgaa gaaaatcaaa 55080 gctcaagcat ctgataaaca tgaagatgac aagaactatg atgtaattca tccagatatt 55140 gcagttggta acggttcttt tggtagcgtg attctttcta ctcacttcta cacttttgag 55200 aacaatgttc tgacaaaacc tattcaggaa cagtttatca ttgatactct tgtaccttac 55260 actggtggta acggtgctaa cggagaacct gaactggatg aagacgaact ggctatgctt 55320 ggtcttgatg gtgtcgaaga taacggtgaa atcactgaag aagacgcaac agaccataaa 55380 tcttcgaatg attctgatga tggtgatgat gaagatttgc cagacccaga tgacgaagaa 55440 gatgaagact tcgatacaga agattaatct ctaaagttac tttaaagccc tgtactccgt 55500 atggggcttt ttcatatgga gagccataat ggagaagtac acattaacaa aacttcccga 55560 ttcagttaca catgttttta ttgactctga cagtattgcc tataaaggtg cttgtgtagt 55620 tgagaaagca aagtataaat atgtcaataa actcacagca gaagagtctg agccatttga 55680 taatgcgaaa gatgctgcaa gatggttagc agaccagaga atccttgtgg aagagcttgg 55740 cctgacattt gatgaagatg aatgggaaag acagacttgg aaagaagcta agagtgaaaa 55800 agaagctatc atggctactc aacaggtgct tcaggaatgg cttaaggttg ttggtaaaga 55860 gagaacttgg gtaggttact taacagagaa aggtgtgcat aaacacaaag acattaaagg 55920 tcttgagcac caatatcaag gtaaccgcaa agatgctgtc acaccaacac acttagttgc 55980 ttgtcgtgag tatcttctgt caagaccaga gttcaagctg attcttgatg gatttgaagc 56040 tgactctatc gtaattgcta aagctgaaaa gatgggtaaa aaggctgcct taatgagtat 56100 tgataaagac cttcgacaag ctgaaggtac ttactgcatt gacatgacct atgaaaaatc 56160 acctttaatt tttattgctg ataacaatgt tggtgaaatc tgggattgtc caataaaatc 56220 gacaccaaaa gctaagaaga cagttggggt aggttttaaa tttctttgtt accaagctgt 56280 ggctggtgat aatgctgata attattttgg tttaaagggc gttggtaagg ctactgtaat 56340 gaaagccctt gaagggaaaa caacttacaa agagtgtctt gatgctatct atgaacttta 56400 tgctaagaaa gagtcataca cttatgtctc gtgggatggt caaacaatta ccagaacacc 56460 tttagagtta atgcagcaac actttttctt agcttatcag gaaagaaaca agaaagatga 56520 tttcactttt aagaaatata attgggagcc ttgtatgaat gaagattaaa ggatttggta 56580 attgccccac ctatggtcat tgggtatctt tatgtgggga agttgacccc actaaacatt 56640 ttggttttgt ctatttagta cactgtaaga aaaccggaca atattatata ggaaggaaac 56700 agtttaacag tgttaccaaa agaaaagttg ctggaaagac tcggaagaag gtagtcacta 56760 aggagagtga ttggatgact tatgagactt cttctgagta tattaaaaaa gatattgaga 56820 gctttggaaa agaatttttt gacttttaca tcattcaaac ctactacaca aaaggtggcc 56880 tagtctatgg tgaagcaaac cttcaacata aattcgatgt aatgacaaaa aggattgact 56940 cgaagctcag actcttctac aatgccaata ttgcagcaat taagtttatt actaaagaaa 57000 cttatgaaga tgctgaaaaa agaatccata aggtaatgaa agcaaattgt gcttgataac 57060 tattgagaga gaataaaatg tttaacaaaa gtaaagctgt gagccatgta gcaaaagttg 57120 acagcaagat tgaagaactt gagcgcattc ttgcaaatgc cagagagtca ataattaaag 57180 aagttgaagc tgttgaatct caaatgcagc acctaatgct taaacgtcaa gagttacgtg 57240 agcatctgga atatattgaa acacgagagc taaaagttaa cagattttta aggagccaaa 57300 atgtttaact caagagagtc agtaaaaaac tggaaccttc gttgtggaaa cactcaaaag 57360 caaccttaca gtgatgagta ttgggaatct ttaaaatctc aatctctgtg tatgcttgaa 57420 gaagcaaaag agcttgtaaa agcaattgaa gagaaagacc ccattgagac actggatgct 57480 caggcagatt tgcaatatgt tcttgatggc ttgatttatc tgtcacaaca tgaccataat 57540 ggcgctatga aagctgtttg ccataataat gacctgaagt acacagacga ctatgaagaa 57600 gctttaaaac gtcttgcaga tattgagaag cgtactggtc aagagtgtat catcagaatg 57660 tcagtagttg atggtaaaga atggtatgca attgttcgtg cagccgatgg gaagattatg 57720 aagcaatcta atctccctaa agttcagctt ggtgaataca ttgtagaact tgaaagccaa 57780 gaactttttg tggtagtatc tgacacatgc gttatctgca aaggtattgt atgtagctta 57840 aaggatttgg gtgtagatgg ttttgtagaa gttaatccaa ttacctctaa agcagataaa 57900 gatttctgta aagagaatgg actatggatt gcagatattg tctactatga tggtgagcag 57960 ttccatgtaa cctcataccc gaaactgaat tatgatgcta ataacctgaa gtgctggtta 58020 aaaggggttg gttataatgg attcacagaa cattaataaa gaaggtgtgg ctcagaagag 58080 ccacttctca aactacaata tttctatgac ggtgtttatg aatgacccgt tacttgagaa 58140 gtatggtgag actccagata cacttctgga taatgaacaa gttttaaaag cagtcctgta 58200 caaatacggg attgatattg agaaagagta ttcttttgaa atctgtcaac acaggaatac 58260 ttttggtaaa gttgtaatgg ctccactctt catgggtgta gaaaggactg actatggttg 58320 gttatatcta aaaagaaact tggagaaata ccgtgtctaa agcaaaaaag ttatcttatg 58380 atgacattat ctcaggtgct aaattaggtg ttgatagtat cggacaagat gttaagcacg 58440 gagacacagt tatgtactgt gacgaccgaa gaggcagaag tccaatcttg tttggaagag 58500 ttgtttgcaa gatgcgaggt aattatgttg tttcagatat ggatgtcaac gtcgcacaaa 58560 agctcaaaac aatcatggat agcaagattt catcatggct cttaaattgt atgcacgcat 58620 cgtcaatcac aaaggtaagt gataagtttt acgatatgtg gcagaatgag caaattttca 58680 agatttaaac tagggagcct cttcggaggc tcttttcatc tgtaggattt aaaaatgatt 58740 aagacaatta aaaaatcaaa cggtacagta gtaagctttg acccagaaag actgaataaa 58800 tgggcatcat gggcagataa gcgtggaatt atctggtcag aagtcactat ggaagctatg 58860 aaacgtgtct atgaaggttg caccacaaaa gagatgcacc aagccatgat tgatgtttgt 58920 gttgataagc aaactcaaga gtactcagat atggctggac ggctacttct gggaattatc 58980 tacaaagaag cctttggagg ttttactaag gttcctacgc tggttacctt cgttaaaaat 59040 atggagagag caggactttg ggagaagatg gattattcac aggaagagct tgaatacctg 59100 caaggttaca tcgtgcactc aaaagatatc tcttacggtt atgcagtctt gaaacagttc 59160 agagacaagt atggtatccg tgatattaaa actggaaggc tttttgagtc acctcaattt 59220 atgtttatgg gtatggctat gaaagccttt gagaagcaac caaagcaccg tagactgcaa 59280 gatgttatca agctgtacac ttacctatct gacctgaaga ttaacgctcc tacgccttat 59340 ttaaacggtt taagagcaac taaatcaggc tatgcgtcat gctgtttgat taaggcaaat 59400 gatactgctg aatcacttgg tattgctgca aaggttgctt atgacatgac tacaaagcaa 59460 gctggtattg gtatgttgat ggagactcgc actattggtg atggtatccg tcaaaatact 59520 attgagcaca tgggtaagct accttattac aagcttgtac gttcatctgt agaggcaaac 59580 aaacagaaga gtcgtggtgg ttcagctaat aacttctaca ctgctctaga cccgcagatt 59640 gaagatttac tgcgtttgaa gcaccctaca acggttcctt ctaaacgtat taatgagatg 59700 gactactcat tcggtacaaa cgattatttc tggcagtgtg ttcagtatga tacagattgg 59760 ttgctattct cttacaaaga tgcaccaaaa ctctatgaca tgttctacac agcatctgct 59820 gatgagtttg ctatggcagt tggtcatgca gtacattcag gtgttaaaca cagacgagtc 59880 aaggctcgtg aaattgctaa gctgtttatt caacagcgtt atgctacagg tcgtgtgtat 59940 ccattcttca ctaacaatgc aaacacacat acaccattta aagagccttt gaagatgtca 60000 aatctttgca tggaaattgt gttgccagtg tacggctttg agaaagagac agacctttac 60060 agagacgatg ctgtgaagga ggatggtgag gtagctcttt gcttcctagc tagtttggtt 60120 gcagggagaa tttcagaaga tgaatacgct gacgttgctt attatgctct tgcaatggtt 60180 gactccgtta ttgaccttat ggattatccg tatccgtcga tgcgtaacca tgttcagaag 60240 cgtcgctctg ttggcattgg ccttacaaat gtggctcatt accttgcgaa gaactacgtg 60300 aactactctt cacgagcagg taaaacgaaa cttcatgaac ttgcagagat gcactcttac 60360 tatctgcatg aagcctctct gagacttgct aaagagcgtg gcgttccaga gtacatgaag 60420 ttcactaagt atcctgaagg ctgggttcct ccaaagacag ctaacaagaa gattgatgaa 60480 aagcatgatg caaaactacg atatgattgg gatgacttag cacaacgtat caaagaaaat 60540 ggtggaatcc gaaactctgt attagaagct tacatgccta atgagagttc ttcactagca 60600 actaatacga caaatggctt gtacccaatt cgtgacttta ttttaactaa aaagtctgca 60660 actggtaacg tactgtttat tgttccagat tatgaagaac tgaagtatgt ctatgaaatt 60720 gcttgggata ttgacacctt tgatatgatt gattgttatg caattgttca aaagttcact 60780 ggtcaggcta tctcttcaga cttctatgtt gactatgcaa agtctaagaa ggtatcattg 60840 gctcaagctt tgaagtacat gatttatgcc aactcggtag gtatgaaaac catgtactac 60900 cttaacagtc gcattggtgt aggtaaatct gcactgcaag atgcttattg cgagggttgt 60960 ggtgtttagt tttaacaact atgagggtcg taaaagaccc tctaaaaata actttggaga 61020 gaccatgaaa gatttaatag aaaaacatga gcgacctata tacttgctcc ataagcctcg 61080 gaaaacgatg tactatgtga gtagcacaga catgatgata aaacaaaatg atgactcatg 61140 ggtcgatggc gtctcttaca tctttacagc agatggtaaa atctacgcaa gaccttatga 61200 gatgtttaac aaagaaaact gggaagtttt agacagaaaa caagccctag aaatgataaa 61260 gaaaggagaa atcacgctat gattaaccag cacccaatct ttttaggtgg tgagagaaaa 61320 acttttgact cacttaataa acactaccca aaaatctttg aactttataa acaacaaaaa 61380 gcacaagact ggtcagaaga tgagttccct tttgaacaat cacgtcttga ttttgagagt 61440 gtcccagcat caatgtcagg tgtaatgctt gagattctta agtggcagtg ggaagcagat 61500 acccaagttg ctaagagctt ggcatttgcc tttgcaccct ttatctctga tgacatctat 61560 gcaactgcaa ttatgaagca gtctgagatt gaaaacctac atgctcttac ttactcagag 61620 attgtaaggc aatgtattaa aaaccctgaa accatcttag atgagattaa ccagaatgtt 61680 gctgtgcaag accgattaaa aactgtgaat cgtgttcttg aagaattact ggatgaaggt 61740 ataaactacc gtctgagtta tgtgcgtgat tcacttctgg acaaagaccc tttacacttc 61800 cataaagtga ttctgaaagg tctatttgca gtgactgcac tggaaggtat atcttttatg 61860 gcatcctttg catgtacttt tgcacttgat gctcaagata aatttcaggg tattgctcaa 61920 gctgtccaga aaattatgct tgacgaaatc cttcacacta aaattgatat tgaagtttta 61980 aaagaaactt taagagatga tgagtggcag aaagcttttc aacaaattct tccagagatt 62040 aaagtaatct tagatgaagt agttgaaagt gaagagaaat ggtcgtatta tatcttctcc 62100 gaaggacgtg ctgtagttgg cctaaataca aagcttcttc atgagtgggt ttactataat 62160 gctgccccac tgtatgatat gtttggcatt caaagagatt ttgtagctcc taaagaacca 62220 cctttgaaat atatgattaa gaagatggaa attgataaag agcagaatgc taatcaggag 62280 caacagaatg gcgcgtacct attgaatact gttgtagatg atttgaatag tggattttta 62340 gaggttcctt aatgacttat gtaatttact ccaaagctgg atgccctcag tgtgagactg 62400 caaagaattt tgcaaaagct cgtggtattg gccatgttgt gagaatgtta gggcaggatt 62460 atgaactatc agacctgatg gagattgcac agatgccagt tcgtcagatg ccattcatca 62520 tgaaaactga tggacaaaac ctaaaacctg ttgggacgct acagaatttt atggcagagg 62580 tgaataatgc ttaaacgcat ttgggaaggt ttggttgttg atgcaccagc tattgtgatt 62640 ggtatgctta ttgttaacct atttactgac tttgaacaag gttcattgtt tggagccatg 62700 ttactatggg ttatctttga aatattagag atacagttag gtatcactga aaaactaaga 62760 aaactctttg tgaagttttc taaaaagatt taaaatgaaa gggtctcttc ggagaccttt 62820 ttagcatgta aaggggcaat aatgaacaaa gtacagatta ttaaaaagag tggctcactc 62880 gaagaacctg atatcaaaaa agttttagca gcagtcacaa agtcagctaa tagggttggt 62940 tataaagagc ttccaccaga tgttactcaa gctcttgagt cagcatttat gaggattctg 63000 gtaaaatcta ctaagcagaa taatttactc gtttcagtaa atgatattca cagtattgtt 63060 gagggtgctt tggcagaagt caatcacgag atttatgagt cttactcaac atatagaaat 63120 taccgtaaag aagttgctg aaattgggat gaactttacc agaagaccaa agatacactc 63180 ttcttaggtg acagagagaa tgctaacttt gacagcagtt taatttctac aaaaggttca 63240 attattcgtg gttatctgac taaagaaatc tttaaacagt accatttaac accagaggaa 63300 cttgaagcca ttgaaaaagg gtttatctat atccatgatt taagagacct aatttttggt 63360 ggtatcaact gttgcctgtt tgacattggt aaagtcctga aaggtggctt tgaaatgtct 63420 ggcatcgaat attgtgaacc aaagtctgta ctgtcagcct tacaggttat tggtgatgta 63480 gttctttcag caactgcaca gcaatttggt ggctttactt tagcagagat tgataaggta 63540 cttgtaccgt atgctaagaa gtctctacgc tatcatgctg agaaagcagc atcttatggt 63600 attcctaaag aacattacca caattatgtc atggagcagc tacagattga attaactcaa 63660 ggcttccagt cactagagat gaaactgaat actgtaccat gtagtcgtgg ggattttgca 63720 ttcacaactt tgacattcgg gttacttgac tcagatatgt ccaatgaaga taaccgactg 63780 caatacatga ttgcaagtac tctcctagat gttcgtatga atggacaagg caagagcaag 63840 aaacctgttg tattccctaa actggtttat atctatgacc agaagagaca taatgagaat 63900 atctgtcaag gccaactgta cagcaaagct attgagtgtt gctctaaagc gatgtatcca 63960 gattttttaa gtgtatctgg tcatggtgct gtagcagagg cttttgagcg ttctggtaag 64020 gtaatttcac cgatgggtta acaagctcta tagctcatct aaaacgtgcc taaacaggga 64080 aactctacag gtgtagacaa tcctgtgcta aatgatgtac gatgtaagaa actatataac 64140 ataggagtct tacatgccaa ataacttcga acatgaattt gaatacatct aaatgccgaa 64200 cgactaaccg tgatgaatgt agcggtgtag ggtcaagcga ctcgaaatgg tacgctactt 64260 agaaataagt agaagatata gtctggactt actggcgaca gtaagcagct tgaataaagc 64320 ggggtaagct tagcgaactt acctgaacaa caagtgtaga gcatttttat caccatacca 64380 taacgaatat ggtgaagagt tttatgtagg tcgtgcaaac attggtgctg tatcattgaa 64440 cttaccaatg atttaccagt attctaaaga gaatggttta gatttctgga aagagcttga 64500 taagtaccta gagatgattc gtagtttcca taagaaacgc tatgaaatga ttgccaatat 64560 gccagcaagt tctaaccctc ttgcatttac acaaggtggt ctgtacaaag gaactaagaa 64620 gcctactgac agggttggtt gggatattgt gaagtccttc acagcttctt ttggggttac 64680 tgcgcttgat gagctatctg ttcttgctga aggtaaacga cttcatgaag ttggaagctg 64740 tagttttgca tacgatgttc tggaatacat caacatgaaa actgaagagt ttaaaaatga 64800 agatggcttc ttatatgcag tttatggcac tccagcagaa tcactttgtg gaactcagct 64860 aaaacagttc agagatatgt ttggtgttat caaaggtgtt tctgataagg aatactttac 64920 aaacagtttc catatgaatg ttgcagcaga tattacacca tttgagaagc aggacttaga 64980 agaacctttc ttccatatct gtagaggtgg tagaatccag tatgtcagag tagctaaccc 65040 agaaaactta ccagcactta aaagttgtat tacaagaggg atgttgaaag gtttctatca 65100 gggacttaac tttgacctag caatctgtga acattgtggt aacagaccaa aggctgatgt 65160 tgaagaatgt gaggtttgcc attcacacga tatctctgtg attaacaggg tgtgcgggta 65220 cttgtcattc acaaaaatta aaggtcagtc tcgaatgaat gacgctaaga tggcagaaat 65280 taaggatagg gtttctatgt aaaacttgac atggtggtgt gagtcttgat aggcttgcat 65340 caccttttta tttggagtta cattatggac ttgtggcttt atgtagcagt ttgtgggatt 65400 gtatgtctga taatcttatt tattcttgga gtaatgcgat ttagagacat catacagtca 65460 gattttttgc ctgagtttgc catgatagtt ggggttatta ccctttgtgt cttggcagtg 65520 ttctattcat acaaaactgt gatatggatg gaggcaaaca attgtgtaaa aactcatcag 65580 catcgtgagg ttactcattt tgtgagtgta cttagtggta gcaccactat acttgtccca 65640 caaacttctg tagagtatct ttacacatgt gataatggta aaactatatg gtggaattaa 65700 caatgacaac actcttaaaa gtattgatgc ccatactaca tatagcagtc ttcttctttg 65760 gtattagtgt tatggtatgg ggcttttcag acccacaatg gagtttttca tatcatggtc 65820 agatggactt atggtcatgc ttcaaaccat ttttagggtt agctatagca ttcagtgcat 65880 taccaactaa ggtgaaatta tgattaagtt gaaccaaaaa cagctagagt ggattaaaga 65940 ctacgcctca gagtgtggct cttgtgaaaa gaaccaccta agatactcaa cattccatac 66000 gaccttcaca ttgtatattg gtgacaatgt tctaagtgac tccgtagaag ataatgtagc 66060 attacctaat gagttgcttg acaaattagc tgtagtcact ggaacttggt ctgaagaaga 66120 cggtcatgaa ttatctaacg atgttgtctt ctacagtctt gaaaacatta tgaatccaga 66180 gtacattatg ttaatgactt gtgcacaaga ctgtgtgcca ttacaaaact tcattaaaga 66240 acactgtgaa gagtttatta ctaaacaggt tccttgtcag gtggtgtttg aatgagtaaa 66300 aggcttaaat aatattggtg aggtaactag atgaattaca tggagattag accatttgac 66360 acagctaatg gtgaaggggt tcgtgtaagc ctcttcgtag ctggctgcaa acatcactgt 66420 gaaggctgct ttaacaggga gtcttggaaa tttaatgctg gtaaagagtt tacttatgca 66480 aacctctacg gcatcattaa gttaatggat gataatgcta tcagtggact gtcaatactt 66540 ggtggggagc ctctggatga cagaaacatt caagaggtca ctaatatatg caagcatatt 66600 aaaaccgttt atccagagaa gtctatctgg ctctggacag gttttcagtt acacgaaaaa 66660 attcacctag atgtgatgaa atacgttgac gtggtgattg atggtaagta tgattcatct 66720 aaaccaacag ttaagccgta tcgtggttct gataaccaaa acctctggag aaaagagtat 66780 gggtggcaag gtgactgtca gtggagagcc gagtaagtct cgtcgtaaag tgggttacag 66840 aaaaggtgag ataatggttc tttcttttaa cagtgcttta ttgggtactt ataaaattaa 66900 gaccgagcta cttgatgaat ggagaaagta tacaaactcc cttttcggta atgttggaat 66960 ggaagcctta gcagagattg agaaggctgg aacatttgaa gttgttggta aaggtaacta 67020 cacaatgttc tacaaaacaa atttaaacgt tggtgacagt attgttggtg tcactgaaga 67080 agaacttaaa acattttgtg aaaagattga gagagatgcg aaatgggact ctggaacagt 67140 tttaaatcaa aagtaaaagc agcactagca tcattaggca tactcactgg cattgatgtg 67200 actcatctta tgcagaactt taagatggat gagaagctgg ctaatgagat tatgaaaagt 67260 atctatgtag gccgtggcaa aggtggtaag aaacaagccc accgtccgtc tggtgcagca 67320 gctattaaac gagcagctaa gaaagctcgt aaccgtaaac gtaataagaa ggctaaataa 67380 tcatgagcaa agtttataac actcgtaaat tacagatttt cgtactgtgc cagtttatgg 67440 ctaaagaata taacttctac tattgtggta caggttttat tagcgatgat gatggctcat 67500 acctcccatt taaagaagct gtgaaactct ttaatgaaga gaaaagctct aagaaagata 67560 ttgaaaaggt taagctaacc tacagcaaga aagataagaa gattatctgc ctagataact 67620 ttgtgaaagt tactggagaa gcaaaagagt ttatggatga gagtgaaatc tcattcacaa 67680 gcatcttgaa agtagctcag taaacagata ctataagggg actgtaaagg ttcccttttt 67740 attttggaga taatatgtat ctgtcaaatc tgaaacgttc tgttgcaatg tcagtgttaa 67800 gactcagttt tgaggaacgt caagagttca ttgactccca ccaatacgac ccttccaact 67860 ccaatcacat gattctctgg aatcgtgaga gtactcgtga aagagcatta tttcgatatt 67920 atccacacta caccatagac aacctgtatg aatgttttgt tgtgaagaac accattaaag 67980 tactcaataa actttgcaga tacacaggca gtcaatcctt cacattaggc caccacaaac 68040 ctgttacaaa aggtggtgaa catcactgtg caaactggtt tattcagact aaagctgata 68100 accagaagca aggagatagc ctcctaagta tccctaagat gacctatgaa gagcaagaga 68160 gatatatcaa aaataatatg ccagatgtgc ttgacaacaa ctatacagat ttggcaatat 68220 ctctcctgtt gaagttcgag atagtttata gggcaactta caatggctaa agaaaagtgg 68280 gagattttac cattagtcag tgaaggcggt aatggttgtg aaatgtacat gatacgtggt 68340 catgttccag agccaattgc acttgagatg gtaaataatt atacagatgg ttcctgcaaa 68400 gacttaggag aaccaactgt caagcagcaa tgggtaaagc ctgtgccaga tagtacagga 68460 aactgtagtg tactttatca cgttgtagac cctgtaaaat gcaagtctgc aatggcagta 68520 actaacgtaa cttttgattg agagagaaaa ttatgaaaac atctatacgt gttacagttc 68580 attcaccaac taaaggaact catgaagaag agtttaacat catccaattt ccttctggtg 68640 agattggtgg acacttttca ccagagtttg ttgattttac tgcttatgca gcatcatcta 68700 tcaacaatgt gattatgatt gtaaaaggtt atgataagga cacattgttt gctgtggcac 68760 ttgctaaaga agcaattgat gatttagtac ctcataagtt tgctatgaag actgctatct 68820 tttactattt gccaaatgca cgttacgacc gtcacatgtt taaaggtgat gcagcagctt 68880 tgaaagtgtt tgcacaacag gttaatgcaa tgggttttga tgcagtctgt gcagttgacc 68940 ctcacagcta tgtaccagat aacctgttta actgcttcca gagtattcct caaaaggaaa 69000 ttgcagtcca ctatgcaaat gacccacgca ttgatttctt ggtagcccca gatgcaggtg 69060 cagctaagaa gactgaagag actgctaaag aggtggataa accatacatc acaatgtcta 69120 aagtacgtaa ccttaagact ggtgaaatta ctggtatgcg aatccttgat gatgttgatt 69180 tgaccgataa aactgtcatg attctcgatg atatctgtga tggtggtcga accttcatag 69240 aagcagctaa acatcttcgt gaagcaggtg caaaacgtgt ggaactctat gtaactcatg 69300 gcattttctc taaaggtgtt gaaaacttac ttgacaatgg tattgaccat atctacacta 69360 caaactcttt aggggaagct aaagaccgtg gcttaacaca ttatgcccaa gttactgtaa 69420 ttaccattga ttgaaacttt atagggggtt tcaaagcccc attttgagag agattaaaaa 69480 gatgactaaa tcactttatg cagtaccagc aggtttaaac gcggatgctt acaaatctgg 69540 ccatatttat cagtatccta gtgcaacaga gtacctaatg ttcaacctga caccacgtag 69600 tgacaaatgg tttaacagcc ctttagcaat tgacggtgta gtggcttttg gtattcaacg 69660 ttttgttaaa gattacctga tagaccactg gaacgccacc ttctttgaac gtgacaaaaa 69720 agaagcaatt gacgaaatct tagaagtcat gaacggtgtt ctgggtaaag atgctattgg 69780 tcgagaacat tgggaagcac tccatgactt aggttatcta ccagttgaag tatacgctgt 69840 agaagaaggc acagttgttc ctatgcgtgt accaatgatt gtcttccaga acactgtctc 69900 aggtttccat tgggtagcgg ggtatctgga agatgctttc tctgctgaga tttggaaggc 69960 ttgtaccatt gcaactattg cattgcacta caaacgtatc tgtaagaagt ggtctgacct 70020 tacttgtgac aacgacttgc atttgcctta tcagtgccat gactttgcta tgcgtggtat 70080 gtccggcttt accgatgacg catttaacgc tgtaggccac ttaaccagct ttaaaggaac 70140 tgatagcttc cctgctgtgt atacagctaa acgcatctat ggacagtcct acccaatctc 70200 tgatattggt agctctgtac cagccactga acactctgta atgtgtgcaa acattgcttg 70260 ggaaggtggt agtgagttga ttgaagaaga aagacgcttt aaaggtgagt tacaaacctt 70320 ccgtcgtttc ttaacagaaa cttacccaac tggtattgca agtgttgttt cagacactta 70380 taacttctgg agaactgtat cagtcatatt accagaactc cgtaaagaga ttatggaacg 70440 taatggcaaa ctagtaattc gtcctgattc tggagaccct gtacatattg tcacaggata 70500 caaagcaatc catttagagt gtgctaaaaa ggcttattac gaacacctaa gtaagctgga 70560 agccagcgac acaatgctga atgctgttct gaacatgaag cttgaaaaca tcaattatgg 70620 tattgctgga tggctactgt cagaaggtta cgaaatggtt gttgacagag aagactttga 70680 agttgctgat acagtgctgt tgaaaaatgc ttatatggtt ggttctgcaa acgttgtaac 70740 acgtcctgta gcggagattg atggagctat caagacactg tataacatct ttggaggaac 70800 tatcaactct aaaggtttta aagtactgga tgagcatatc gggcttattt atggtgactc 70860 tatcacgttg gaacgtgcaa acgaaatact gaagcgtttg tatgaaatgg gttttgcaag 70920 ctctaacgta gtatttggtg taggttctta cacttaccag tacatgactc gtgacacttt 70980 tgcatttgct gtcaaagcaa ctcttgcaag tattggtggt aaagagatta tgcttgcaaa 71040 agaccctaaa acagatagtg gtgttaagaa gtctgctttt ggtggtgtag cacctatgtg 71100 ggatggtgaa aatcttaaag ctgtagatgg ctatggattc cagagctttg cagatgcact 71160 tgaacaccca gcttgtgctt tacgtctagt ctttagcgac tctgagcagt tcggttacac 71220 aactcttggt gatattcgaa ataatattga caagcagctt taaaagtata tgataagagg 71280 ctcctacggg agccttttta atttctggag aagattatga aaatcaaaga gatgaacatc 71340 aacattgtct tagaagagcg ttgggagaac atcaagaagc ctgaaaatgg tcataagttc 71400 ctcaataaaa tcttagtagc agctaaagaa gaactgactg gcaagattgc agcagcaatc 71460 acaataaagg tctgtgtaaa aggtcttcca gacaatcacc aatttgcact tgacgagttt 71520 aaagagagct tctacaatcc aaacaaacag atgcttgaaa gtaactttgc agtgtctaca 71580 agtatcgtcc atgacagaag ctttatcctt tacaagaata tgagaggtga gtcatgcaag 71640 catattggat agaaattttg ctatcacttg gtagtgtagc agtgtttgtt tatcttcttt 71700 gtaagtactt tgcagagtac aagaagtgtg attactgcaa cggagaaggg tatacaagag 71760 caggttgttg ccctatgtgt ggcggttctg gtaaaatgtt taataagtaa tttaacagta 71820 aactaagagg aaagtattat gcgcatggta aatgaccacg cagaagtgat taagagttca 71880 acttctttag agacgtctca agcacagatt acaatgacac ctgagatgtt cagccttttg 71940 agttctggtg tatatacctt taaagaaagg gcagtgattc gtgaactgtc atgtaacgca 72000 gtagatgctc agaaagaagc tggaaaagag aacatcccgt tccatgtgca tttacctact 72060 cgttttgagc cttactttga agttcgtgat tttggaactg gattgactca tgataaagtt 72120 atgagtttgt acctaaacta cggggcttct acgaagaatg actctaatga ctacattggt 72180 gcaatgggta tcggctcaaa atccccattt gcaattgctc agtcattcac agtatccagc 72240 tatgttgacg gtgttgttaa caagtactct gtttaccttg agaatggtat ccctcaagta 72300 actaagctaa ccaccaaccc aacgaatgaa cctaatggtt tagctgtacg tgtagcagtt 72360 gctgaccatc gtatttcaaa gttctttgaa gaggctggta atgtatactc atactttgct 72420 gtaaaaccag aaagtaatat tgagtatgac gatgtattag cagatatgaa cgtcattgcc 72480 cgtgaagaag gtgtctatga tgccatgatt cataagcaaa gctggcgttc tagtggtaac 72540 aggacagagt ttaatgtggt gatgggtaac attgcctacc ctgttaatat ggaagcatta 72600 cttggtgatg atttcttcaa agtattgcca gaatttttcc gtaggagcgt agaccttgta 72660 aacatctaca tgcctattgg ttcagttgct attgcagctt ctcgtgaagc attgcagatg 72720 aatgacacga caaaaaatgt tatcattgag gctactaaaa agataactga agcaattaca 72780 aaggatgtta tcaagaaagt taatagccaa cctacactca tggatgctgc acaggcttat 72840 gctgagttac gtttgaactc acgagagatg tttaatgctg tgtgtccaaa gctagagtgg 72900 ggaggcgtta agcttgattc tctggaagaa gaattactaa acattcgtcg tggaattatc 72960 tacgcagaag acggctcagt tgtctatgaa cgcgatggta aaggtaatat taaggttgat 73020 aggaacggaa acaacatccc taaagtagat tatctttata atccagttgc ttatgttaag 73080 tttaactctt tagagagtaa gattcgtgca acagcacttt cctacactca agaggcgagt 73140 atgttcaata tttttggtgc aatgcgcaaa agccaaattg aacagttttt gtttgttatc 73200 aatgaccgtc gcaataaaaa tggtactgag aagactgtag gacgcaatca gattctgcgt 73260 ggtgcatgtc gagactatgc cagtgagtca agtctgtttc acagatacaa tggtattgtc 73320 tttgtattct cgactgaaaa agagttagac gacctaatca atctgcacaa acttgataaa 73380 agcttgttaa agattgtgaa gatgtctgat aaggaacacc attatcagcg taaagaagct 73440 gtaagaggtg ttgtaaaact ttggaaagct gttccagcag aaggtgtagc ttcctataaa 73500 gaggtttcag aggactttga tacaattgaa gaacctcagt tttatattaa ggcagtaggc 73560 gacacagttg atggcgaatg tttttgctca tctccagaag atgtagctaa gtcagttgcc 73620 aatgtcattg gaaagacagt atatgttttc cgaaaagcaa attggaaaaa gataccagaa 73680 gactggattg aagtagatga gaagctcttg aacgacagct taactgatgt tcattggatt 73740 aatcataaca gatatatgac acgtatctat atgaatggtg tcctcgacct tacaagcagt 73800 tggattattg ccagaaactt tacgttcaat aacagaaaga tttctcgtgg ttattgttat 73860 tcacgagata caaacaaaac tatcttctta gaaggtaatg aagatgctgt tgaggcaatg 73920 tttggtaaaa tccaatacgt tgctgcaccg tttgcataca cttacactat tagtaggttg 73980 caaactttga aagattgcct tgacaatgat acaaagctgt acaagaaaat taagaaagct 74040 ggtgaccgta tggtcatcaa ggtgacaaac tatctttcaa aaagaaaaca agaaaacttc 74100 ttgctttctc atttagattg gaataaagtg tcacctattg aagtgagtaa gttcttaggc 74160 tttgatgtga agtgtgttcc agaaggaact acaatttacg attaaagtgt ttgacaaggg 74220 gcttcaaagc cccttagaat agccttacaa aatgatttat taactaacaa gagagagtaa 74280 taagatgact actaaaacta aagcacagat tgacgcagaa atttacaaac tggttaaaga 74340 aggcaagctg actaaaacag ctattgcaca gaagttcaat acttcaaccc gttcagttgg 74400 tcgtgctgta gagcgtcatg aagcaacctt aaagggtaag aaaaagacag cttcgacacc 74460 agctacgaag actttgaagc aagttgctaa gacctttaag aagaaagctg gcaaaccagt 74520 tgaaaaggtc gttaaagaca ctgtacagaa agttccagta aataaactgc acgaagctat 74580 gcagaaagat gacaagattg agtacatgat tactggcgac tctgtaatta tgacttacgg 74640 ttcagaatct gaaattgttg agtctactca tccgaactat caagagattg tagtccatgt 74700 tgtgaaaggt gagttcaaga aagctttcga actgatgaac attcgtaaat ctatcgaaaa 74760 cttcactcag ggtgctatca caatcaaagg tgacaagcta ttctacggtg ctgttgaaat 74820 gcgttctact ctggttgacc gtatccttca catgatgaag actggtgata aaggttttga 74880 acgacttgta atgttcttcg aaaaactgat ggaaaaccca tctaaagact ctgtagaaca 74940 actttgggga ttcgtatctc accttgatgt tgaaattgat gaagaaggct acatcattgg 75000 ttggaagaaa gtctctactc gtgaaggcaa gctggttgac tctcacacct acaaagtacc 75060 taatgattta ggtaacattg tagaaatgcc acgttggatg gttgataaca accgtaacgt 75120 aacttgctct cagggtcttc atgttggtgc ttgggattat gttcgttgct tctcaggtga 75180 tacaatcctg aaggttcgtg tccatccacg agatgttgta tctgttccga ctgattacaa 75240 cgatatgaag atgcgttcat cccgttatga agttgcagca atcgttgata atcaacgtaa 75300 agtgttgaaa gcatgggacg gtaagactga agctttgcat gtcatcgtcg gtactgctgg 75360 tgaactcatc tcccaacgta aacgtgaaat ctaataagta atttcttaaa aggctgcttc 75420 ggcggccttt tttatttgta ttttgtgcag agtgctgtat aattgttgtc acgataaact 75480 aaacaggaga accaatgaag aaattgattc taggattgtg cttaatgttt acagcacatt 75540 tatcttatgc agttgactgc ccagagctat caattagcca aaaagttaac atgttaaaag 75600 cttaccagta tggtgagaac aacatgggga aaggctgggg tattactcta gcagctatag 75660 ccttacaaga gtcagagtta ggtctgaagg tagagaataa aaagacccat gactatggta 75720 ttttccagaa tcacttgaag actgttgtaa agcgtaacaa aattagtcct aatgtggcta 75780 aaaagaaact cttaaaagat tttgactatg cagcaaaaga gacacataaa gagcttgagt 75840 tttggactaa ggtacatggt cacccgaagt caaagaagac tttacaaaaa gttttagcat 75900 catataatgc tgggtattcg tacaaaatcc ctaaagctaa gaaatactct caagatgtct 75960 ataacaatat gaaagttatt gctcaatgtg aatttgcaac aaacatttct aaggtaaatc 76020 atgaaaaaat taagaaagcc tgatgaagtc ctgtgccacg cttatgactt gcatcctcat 76080 gagttaggtc ttgattcctg tgtatggact ccagaacagt gcagggattt tgaagacact 76140 gcaagagaag ttgtatgttc acttgaagag ttccacacat cagaaccaat tgtgaacgtt 76200 gtggataaag aaactggaca agctataggt gtaagtcgtg atagtttagt catagtcaat 76260 aaagaccttg tagaaaaagg taacctcatc ttagcagata ttgatggtgt tcttacaaat 76320 tttaatcacg aagattgctc aacggaattg actgatgggt cattctcaca gtacactaat 76380 cttcttgact ctgtaagagc aaaaccaaca tatgttttta gtattattga tgcaattgct 76440 aatcatgcta ccattggact cttgacagct agaggtgaat ctcagagaat acctactgag 76500 atgtttttaa ggcataacat agagcatgat tacatgcttt ttatgcgtgg ttttggaact 76560 aactctataa gtgcagaaag tttaaaagct aggatgattc agtcttgcat tcttccttac 76620 tttaatatag tatgctttat agaagataca gagaagaatg ttcagaaggt aaacagaatc 76680 cttccacaca ttaaaaccat gttagctaaa cattgagaga aaaacttatg aacaatatca 76740 ttacggtagc actggacgat acgacaaaca aatctgtagt agttcgtaaa ttgattcaag 76800 gtaactttga aggtaaaatt tttcgtgctg tcaatgtaaa agctgatggc agcattcgtg 76860 aatatcgtgc attgttgaac gttaagaagc acgtaaaagg tgttggttca acgactgcac 76920 acaaagaaaa cctgatgact atctatgata tgggtaaggc ttcagagtta ggtgctgaag 76980 gtatcgttaa agaaggtgct ccgtatcgct ctttcaatct ggaaactact cttatgcttt 77040 cctttacgag tggttctaaa acaatcactt atctctttac tgatgctgca acggtatctg 77100 ctattaagga tggcactgtc aaagctggcg tagttgctgc tgcaaaagct tcttcgatgg 77160 ctgcaaatat ccttgctaaa gtcctcggtt aaggttaaga tacaggctcc ttcgggagcc 77220 tttttcattt caggagattg ttaaatgaca tttaaagagt tctgtcaagc tactttcata 77280 attgttttct tagttggggc aggtgtctgg ggaggatact cttacagaga ctaccaagtt 77340 gctgaagctg agctaaataa tgagaaacta ataagtgttg ctaaagatgc ttatcaggaa 77400 ggtttagcca cactcagcac caattacaaa aatgatttga aagacgtgct tgctaagaat 77460 aagcatacaa aagaggtact aacgtatgaa aagactaagc cagagtttta taatgtttgt 77520 gttactgata attatgtcag ggtgttcaac gaacaaagtg aacagtacat tcaaaaactc 77580 ccaagtaagt gagagtgaca agtacactca agaagattca agattcactg tcaaaggtaa 77640 tactggtagt gatgtggcat cagcccttga gttttaccgt gatggttttt accagtgcac 77700 aatcaaagct aataacctta ttgatatgat tttattagga aacaaacagc aatgacagag 77760 aatgaagaca ctttctatgt agaagggtac ttactgctac cacgaccaaa agaaacttat 77820 atgcgggttg atttctcacc aaccattatg gataacgtga tgtgtcatat ctttatgcaa 77880 ggtgtcacag cacaacttaa gcatgttggt aaagagtgca aaataaaggt tgacactcat 77940 ccagaaatca aagagaatca ctacacatgg ttcttaccag actctaaaga aatcttagca 78000 gttcttaaaa cgaggaaatg attatgcaat tccagccaac aaatcgtggg ttttcttatg 78060 cagaatttaa agatgctaaa ggagagattt gtactttaca agaaagttca tcatatgaac 78120 catgtatctg gttaggtgct aaagaggtct ctgttcagta cctagatggt ggctggaaaa 78180 cacttgatat cccagcatta gtaggcaaag attttgtagg taaccaaaga atgaaactca 78240 accaagaaca ggtgagagag ttattaccat accttcagta ctttgctgaa aatggtgaat 78300 taccagcaga ggaacttaca aatgcagatt aatggaagag attttgtagc cgtttactac 78360 gagaacgata aagaagttgg cgtagcacaa gtatcttatg gcaatggtaa gtggttgtat 78420 gggacaatcg cagtagtcgg aacaagaagt gatacaaaaa cttttaaaga ctgtgttgac 78480 cttcttgaag aatccatcca caatcattgg tgtctgatat ggatgactga caacgaagtg 78540 atagaacgct tcaaaaagat tggtattaac attgatagca ttgagcatgt tgatttatac 78600 gagctaactg agaaggtaaa ctatgaaagt aatactagcc agagataaaa agacccgtaa 78660 gattgtacgt tcagcagtat tcatagggag agatgaagtc gtcccgtttg caggggaaga 78720 tgtgttaact tataagaagg gcttaaaacg taaattcagt gaccgtttac gtggcttcat 78780 ctctgaacca ttagaagatt tcaaaagtcg ctgtggcgaa ggcatcatca ttcaagaggc 78840 aattattcat ggctgatttc tgcaaagatt gtgctatcga aatgttcgga cgtgatacag 78900 gtgacttaaa aggtctcatc actgaagatg actttaaagc tggttatgca atgccagtaa 78960 tctgtgaagg ttgtggatgt atctgggtag accatgaagg acaacgtgta aaaccttcag 79020 aagataaaga atcttgggag agatgttaaa tgggtattgt aaaaatcatc aatggggata 79080 tcttctcagc atttgataaa ggtaagtttg atattattgg tcacggttgc aactgtatga 79140 atttaatggg tgcaggtatc gctgacaaga tttctaaact ttatccaaaa gcatatgaga 79200 ccgatacaga agtttatctg tatgcaggtg gcataggcca caaaccttgt gaaaatttac 79260 tagggaattt ctctgtagca cgtttaaaac aaggccgtat agctaacctt tacactcagc 79320 ttaagactgg taaagatgcc cgatacagtg ctttagagtc atctttgaaa caacttaaca 79380 gatactgtga agtcaatcag ttgaagaaag ttgggttacc tatgattggt gcaggtatcg 79440 gtggactaga ccctcaagcc gtcgcagtta tcattaatca ggtgatgaag agtgtagatg 79500 tgtatctgta tgtctacgaa ggcgagatgt accacaagtt acgctcaggt tggaagaact 79560 actgtgaacc agaatacttt gcaggtgtag taatgttcac agatgataca gttaccctct 79620 tcagacgaag aaaaggtaag atacatcaaa gtaaccctcc ggttgagaag atgtctctaa 79680 gt; gtgatgcaga tacatatatc tatgcaagaa ctgatgagga tgttgaggaa attttctctt 79800 caccggaagt taccttctta gacgcaaaga actaagaaac tctgtagaat attcacaata 79860 gactaagcct ttcttatctg gtaaattttt caggtaagga gggctttttc atttcaggat 79920 tgtgcaggta gagttctgta gagaaatctg tagagaaaat tttgaactcc aaagagaaaa 79980 ttcattttca aactgtgcag gtaatctcca aagagctttt tcattttcaa aacgtgcaga 80040 ccccgctcag gtcgggcaag aaaaatcatt ttagaattgt gcaggtaggt gggtgcaggt 80100 ggggatgtcg ccatttctca caaggctacc acatctggaa ataaaagtca acagaaaaat 80160 ttgtagcgga ttgaaaaata attcttgatt gtagttctgt tttgtggtag tcgcgtgcgc 80220 ccgtttcatt acagtttttt gcaaaaatat ttttaaaatt tttcttgact ctctgactat 80280 ggttatttaa tatttgcatc aacgggggcg atgacctccc agctaactaa ccttaaacta 80340 ggagattcaa aaatggctta tcgtgcacct aaattcatca acaaagacaa ctttcgcaac 80400 gtactggaga aggtacttga tgaaaaattt aacggtaaaa taattgttgt tcatgcgttc 80460 aatttcaagt atgatatcaa cgggaataaa atcaaccatt acacggcaac catgttagat 80520 gatacgctat caagtgaaaa agccgtactg aatgcactgg caggacgcgg aaaggtatta 80580 gtaagatgcg ataagagacg ttatcaaggc ggcgcatatg gttacgatga tgctgtttat 80640 catcttgaaa atatgggcta ttcagtggag aaagctggag cgtctcaaat aatcggtagt 80700 gatggttatg tgacaatttt caaaattaac tgataaagta ctttacaagg ggcttatagt 80760 tcgataaagt aagccccaga taaagagctt taccactaaa tcctaaattg gagattcaaa 80820 aatggctact atcaaaaacg ttgtaattcc tgtaaaaact cgtgatgacg ctcgcatcat 80880 ggcgaaaaag ttaggcggga aagtggttga caacggaaaa caatctgctg taagatgggg 80940 tgtgaaggct gataaacagt tgaagttaaa aaattcacca ataaatcttt ttacatgtgt 81000 aaacactatc ggaaagacaa atgtatacac gaaaaaggca tatattagac gcgtagcctt 81060 aacatctcca attcgtacaa tgagaggtta tgcaaagctg aaaattagct gataaagtac 81120 tttacaaggg gcttaaaagt tggtatctta agccccagat aaagagcttt accactcaat 81180 cctaaactgg agattcaaaa atgaaattat caaataaagt tcgtgaaaat ctaagcaaat 81240 tactcaacaa aattgatttc attgaaacat caagaaatag ccatgatgtt atggaatggt 81300 tacacggcaa aataaaaggt cgtcgtgtag tgatttgctt atggaatagt tgcagggagt 81360 ataaaaaacc tgtattgcaa gttaatatct atgatgatac tcttaaaagt cctgtcaaat 81420 ctaaaagcga tttactagat tcttatgaga taactgtaaa tggcaaagta tcacgtaaag 81480 aggaagcacc atgttaatga ctggtatcac tgcaatcatt gccattttcg cgctctataa 81540 ggcatataaa gcctataatc tagcaaataa ggcaatcaca cagcaggtta gcaataacct 81600 tgtaatgagc tttctagagc ggttgagcga tgaacaatta aagcggttag aaatgagctt 81660 cagattcagg gcgagaactt atcaaatcag cgacattttc aaagatgatt ttcaattagt 81720 cgatgattat aattctctga taaatgcttt aaatattagt gacttaaaag actatcatac 81780 tgtaattgtt caagagttga gcaaacgaaa acaaaactgt tgacactgat ttttagatag 81840 attaaattac acatcaacgg ggaggcaata aacaccccac taaataaagt ccttaaacta 81900 ggagattcta aaatgtatac tactaacaac ggtcgcactt taaatgtcac tctacgtcac 81960 tatgtagacg gtgtgatgca ttttgaggat ttacaggctg agcaacatat cttagattgg 82020 caagtcgcag gactccaaaa aactgctacc ggatacggca aaaagattcc aactagctgg 82080 aaagtatact atgaaggacg tttgcgcaga atttatcagg atgtatgtag caatagcaca 82140 tcaagttaca tcatagtaaa aggtaaaaaa ctgcatttag tataaagtac tttacagggg 82200 gcttattagt ctgtacagta agccccagat aaagagcttt accactcaat cctaaattgg 82260 agattctaaa atggctatta ataatcgtga gttgtcaatc ttaaaagcac gtttaaccgt 82320 taaccgaatc aatgttatta catcatcagc accggatgaa acattgcaca acattatcgg 82380 aaagattcaa agcgttatct tagacgttga aaacgtaaaa aactcattgg ctgacgttgc 82440 agcaggtatc acgctagacg gtgcacaata tgaaatggtt gacatgttag gcaaatctaa 82500 ggtaatgaat aaagagttag acttgaaaat gtttagattt gccgtgaaag tgtggctatc 82560 tgttgagtat gatgctaatt ttgcaatcgc tgatttcttt gccacttggt tacaacgtaa 82620 tttggcaaat catgactttc gtgacatctg cgacgcaatt tatgcagaac tctaaaaatt 82680 ttattgacac tgatttttag atagattaaa ttacacatca acggggaggg aatgaccttc 82740 cccactaagt aaagtcctta aactaggaga ttcaaaatgg ctattatcaa cggtttaacc 82800 attgaaacca cgcacatcaa agacattaaa gttggtgatg tagttctttc ccacggcgta 82860 gaaaaaacag tcactgcaaa ggatatcaaa gatgattctt tcatgggtag aacactcttc 82920 ggtgattctt attgcttagg ttatcttgca gttttaaaag ttgtcaaaaa ttaactgata 82980 aagtacttta caaggggctt atagttcgat aaagtaagcc ccagataaag agctttacca 83040 ctcaatccta aactggagat tctaaaatgg cttatgtaac cgtaattacc gataaagctg 83100 gctcatcttg gtcaactcaa gtaagtgata agatgtcacc tatgcagtgc ctaaaatact 83160 ttgaacagtg gaacaaaggc gagggtgtaa gcccctttca agtgatgcaa atcatccata 83220 cagataacga agggaacaaa acgaccttga acagtgaata ctacgcaagc cgttttgaaa 83280 cgagaagtaa aacaatgaag cttttacgtg aatccggcta ttctcatatt gccgcgctaa 83340 tctgggatga cctgctaaaa agtcagcgta tcagctatgt aaagcctgaa aaaatcttca 83400 taagttaatc aataacttac aaaaaacttt taaaaaagtg ttgacaatac ccattgacat 83460 tggtaacttt gttcgagggg ggttatctaa aagggattca cttaatagat tctttataga 83520 taaacttaaa aaattgcttg caaaggcaaa tcaaaacaag taaattacta atcaacggga 83580 agggattaac cttcccacta agtaaagtcc ttaaactagg agattcaaaa tgaacaagtt 83640 tcaagctatc aactatattc gctcatccaa cgttatgact aagcccgtta aagatacctt 83700 cgaatttcgt tgcaacggtg tacactttgc aactatcact aaatcagaaa acggggctta 83760 ttatatacat cgtcgtaatg cctctactgt tgtagtctct cactttatgc aagctgtagc 83820 agaactgtta ccgctattct taggtattta cttagatgag tgcaaaaacg ttcgcaacca 83880 cgttaaagac cttttaaatg gttataaaat ggcttatgaa cgttctatca agaaccttaa 83940 caagttctat aaagcccctg aaaatactgt ttcaatggct tacagtgtaa gcggtgagtt 84000 ggttagcctc tttgaagtgg aagaaatcgc gttgcatgac ttacgcccta gcggttactc 84060 taagacacta gaggaaggtt taacgttcaa tatcaagaac ttagaaaaaa ctttaaaaga 84120 tattgaaaaa gatattgacg agacaatcaa aaatctgtaa attgtcaatc aacgggggat 84180 aacctccccc actaaacaaa atcctaaact ggagttatca agatggaaaa cgtagaatta 84240 cacatta agcgatttgt tcaatggtga aatcattaaa gaatcttacg aagttgtcgg attcaaagat 84360 ggtaaagcat ttggcttttt tgacctcgac aaaagcaaag cggttacaat gtacgatgtt 84420 ctatcttgtg aaattctgac aaaatacaac gaaaaaaggc ttgcaaccta cgccaatatg 84480 cagtaaatta ctaatcaacg gggagggaat gaccttccca ctaaacaaag tcctaaactg 84540 gagaatctaa attatgtcaa ctatccgcgt aactgttaat gcttatggct gccacaaact 84600 tgatgcaaaa ttcgatatcg gtggaacaga taacaccttt gaccttatca aaatgctgat 84660 tcagcaggtg ataccagatt ataaccctac tgatgacttc tggaaagatg ccgtaaaaga 84720 agccataaac gaggaatctc actaccatta ttggggtgac ttgattgtct ctctgtacta 84780 cagcaaacaa ggttacaact aatcgacgca gcggggctaa atagccccag atacctgaaa 84840 agctccttct cataggggct tttttacgtc tataaagaga ctctatacaa tccctttcct 84900 ttccttccta gaatctatcc cgtaccttaa aagcaataac tctatagctt actctatagc 84960 cccttatcaa atcaacttaa atataagccc ataacccgaa agggaagggc aaccatttaa 85020 acgctctata aagccctata acagccttat caacctaact taagcaatca tattaaccca 85080 ctctaaaaag ccctttatag agctttctag agccttaaaa gaatcttata agggtgtgag 85140 ctttatcatc tcgttagcta tcttgtgaac tactaaaaag ggcttgttag tgtcttaaaa 85200 gtatgttata ggggctataa agttactttg aagtagctta aaagttacgt aaatgctagc 85260                                                                        85260

Claims (11)

시가톡신 생성 대장균(Shiga Toxin-producing Escherichia coli, STEC)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 ECOH7.
Shiga Toxin-producing Escherichia Bacteriophage ECOH7 consisting of the nucleotide sequence shown in SEQ ID NO: 1, having the killing activity specific for E. coli , STEC.
제1항에 있어서,
상기 시가톡신 생성 대장균은 장관출혈성 대장균(Enterohemorrhagic Escherichia coli, EHEC)인, 박테리오파지 ECOH7.
The method according to claim 1,
The E. coli Shiga toxin produced Minister haemorrhagic Escherichia coli (Enterohemorrhagic Escherichia E. coli , EHEC), bacteriophage ECOH7.
제2항에 있어서,
상기 장관출혈성 대장균은 대장균 O157:H7인, 박테리오파지 ECOH7.
3. The method of claim 2,
The intestinal hemorrhagic Escherichia coli is E. coli O157: H7, bacteriophage ECOH7.
제1항에 있어서,
상기 박테리오파지는 기탁번호 KFCC11732P인, 박테리오파지 ECOH7.
The method according to claim 1,
The bacteriophage is the bacteriophage ECOH7, which is the accession number KFCC11732P.
제1항의 박테리오파지 ECOH7을 포함하는, 항생용 조성물.
7. An antibiotic composition comprising the bacteriophage ECOH7 of claim 1.
제1항의 박테리오파지 ECOH7을 포함하는, 사료 첨가용 조성물.
A composition for feed addition comprising the bacteriophage ECOH7 of claim 1.
제6항의 사료 첨가용 조성물을 포함하는, 사료.
A feed comprising the feed additive composition of claim 6.
제1항의 박테리오파지 ECOH7을 포함하는, 소독제.
A disinfectant comprising bacteriophage ECOH7 of claim 1.
제1항의 박테리오파지 ECOH7을 포함하는, 세척제.
A cleansing agent comprising the bacteriophage ECOH7 of claim 1.
제1항의 박테리오파지 ECOH7을 인간을 제외한 개체에 투여하는 단계를 포함하는, 시가톡신 생성 대장균에 의한 감염성 질병의 예방 또는 치료 방법.
A method for preventing or treating an infectious disease caused by a cytotoxin-producing E. coli, which comprises the step of administering the bacteriophage ECOH7 of claim 1 to an individual other than a human.
제10항에 있어서,
상기 시가톡신 생성 대장균에 의한 감염성 질병은 세균성 식중독, 출혈성 대장염, 용혈성 요독증후군 및 혈전성 혈소판 감소성 자반증으로 이루어진 군에서 선택되는 것인, 시가톡신 생성 대장균에 의한 감염성 질병의 예방 또는 치료 방법.11. The method of claim 10,
Wherein the infectious disease caused by the cytotoxin-producing Escherichia coli is selected from the group consisting of bacterial food poisoning, hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura.
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KR20140140698A (en) * 2013-05-30 2014-12-10 한국외국어대학교 연구산학협력단 Bacteriophage of Escherichia coli and uses thereof

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KR20140140698A (en) * 2013-05-30 2014-12-10 한국외국어대학교 연구산학협력단 Bacteriophage of Escherichia coli and uses thereof

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