KR20190074157A - Composition for improving skin condition comprising chitosan as a effective ingredient and method for preparing the same - Google Patents

Abstract

The present invention relates to a composition for improving skin condition using chitosan as an active ingredient and a method for producing the same. More specifically, the present invention relates to a composition for improving skin condition comprising chitosan having optimized molecular weight and deacetylation as an active ingredient, and a method for producing the same. The composition for improving skin condition according to the present invention is excellent in antimicrobial, anti-inflammatory, and antioxidant effects, and is expected to be useful as a cosmetic, medicine, and food material for improving skin condition.

Description

TECHNICAL FIELD The present invention relates to a composition for improving skin condition comprising chitosan as an active ingredient and a method for producing the same. BACKGROUND ART [0002]

The present invention relates to a composition for improving skin condition using chitosan as an active ingredient and a process for producing the same. More specifically, the present invention relates to a composition for improving skin condition comprising chitosan having optimized molecular weight and deacetylation as an active ingredient, and a method for producing the same. The composition for improving skin condition according to the present invention is antimicrobial, anti-inflammatory, and antioxidative, and is expected to be useful as cosmetics, medicines and food materials for improving skin condition.

Skin is the primary barrier of the human body and protects the organs in the body from the stimulation of the external environment and plays an important role in maintaining the homeostasis of the body. However, as the skin ages, aging progresses gradually. As a result, skin elasticity loss, keratinization, wrinkle formation, skin atrophy, and the like appear. Therefore, attempts have been made to promote collagen synthesis by using retinoic acid and proteins derived from animal placenta as substances for improving skin condition. However, retinoic acid is not stable due to instability of retinoic acid, , And animal placenta-derived proteins have a fatal disadvantage of being able to use bovine extracts from mad cow disease.

On the other hand, chitosan is a natural animal marine polymer having a unique cation in nature and has affinity with human body, has no rejection reaction, no side effects, has no irritation and is a biosurfactant substance and acts on skin to exfoliate, Activity, skin barrier formation, and adsorption of impurities. However, up to now, it has been recognized and used only as a health food that is simply ingested, and development as a cosmetic useful for skin beauty is insufficient.

Accordingly, the present inventors have made intensive efforts to prepare a composition for improving skin condition. As a result, they have found that when controlling the molecular weight and degree of deacetylation (DA: Deacetylation) of chitosan, the antibacterial, anti- And confirming that there is no toxicity and side effects, thereby completing the present invention.

It is an object of the present invention to provide a cosmetic composition for improving skin condition comprising chitosan as an active ingredient.

It is an object of the present invention to provide a pharmaceutical composition for improving skin condition comprising chitosan as an active ingredient.

It is an object of the present invention to provide a skin condition improving food comprising chitosan as an active ingredient.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a concept of the broadest notion that includes scalp and hair, as well as tissue covering the body surface of a face or body.

According to a first embodiment,

The present invention provides a cosmetic composition for improving skin condition comprising chitosan as an active ingredient.

In the cosmetic composition for improving skin condition according to the present invention, the molecular weight of the chitosan is 1 KDa to 100 KDa.

In the cosmetic composition for improving skin condition according to the present invention, the chitosan is characterized by having 50% to 90% deacetylation.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition is characterized by having antibacterial, anti-inflammatory and antioxidative properties.

In the cosmetic composition for skin condition improvement according to the present invention, the cosmetic composition is characterized by exhibiting a skin whitening effect. The skin whitening effect refers to an action of preventing or suppressing the symptoms resulting from the increase of melanin as the production of melanin is inhibited.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition is characterized by exhibiting an effect of improving wrinkles. Such wrinkle improvement includes prevention of wrinkles and removal of wrinkles.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition is characterized by showing skin moisturizing effect. Specifically, the composition according to the present invention is less irritating to the skin, has excellent penetration ability to the skin and excellent water-retaining function in the stratum corneum, and thus has excellent persistence of moisturizing effect.

In the cosmetic composition for skin condition improvement according to the present invention, the cosmetic composition is characterized by exhibiting an effect of preventing skin aging. Specifically, the composition according to the present invention exhibits an antioxidative effect through free radical elimination, and thus can be used for preventing or treating skin aging.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition has little toxicity and side effects, and can be safely used even when taken for a long time.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition may contain, in addition to chitosan, other known compounds or plant extracts known to have a skin condition improving effect. Examples of the compounds and plant extracts already known to have the above skin condition improving effect include mellitus succinic acid, mercaptodextran, teprenone, dihydroxy-isoquinoline, indomethacin, 3-hydroxymannane, vitamin K, But are not limited to, fennel, lemon extract, cucumber extract, rhododendron extract, licorice extract, rosemary extract, acerola cherry extract, bank extract, carob extract or geranium.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition may contain, in addition to chitosan, conventional additives such as stabilizers, solubilizers, surfactants, vitamins, pigments, , And a carrier.

In the cosmetic composition for improving skin condition according to the present invention, the cosmetic composition may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, , Powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it may be manufactured by a formulation of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

According to a second embodiment,

The present invention provides a pharmaceutical composition for treating or improving skin conditions comprising chitosan as an active ingredient.

As used herein, the term "treatment or amelioration" means to stop or delay the progression or onset of disease when used in an object exhibiting an onset symptom.

In the pharmaceutical composition for treating or improving skin condition according to the present invention, the molecular weight of the chitosan is 1 KDa to 100 KDa.

In the pharmaceutical composition for treating or improving skin conditions according to the present invention, the chitosan is characterized by being 50% to 90% deacetylated.

In the pharmaceutical composition for treating or improving skin condition according to the present invention, the pharmaceutical composition is characterized by having antibacterial, anti-inflammatory and antioxidative properties.

In the pharmaceutical composition for treatment or improvement of skin condition according to the present invention, the pharmaceutical composition is characterized by having a skin whitening effect, a wrinkle improving effect, a skin moisturizing effect or a skin aging prevention effect.

In the pharmaceutical composition for treating or improving skin conditions according to the present invention, the pharmaceutical composition may be formulated into various forms for oral and parenteral administration according to a conventional method in the pharmaceutical field. The oral preparation may be formulated into a solid preparation such as powder, granules, tablets, capsules, etc., and a liquid preparation such as a suspension, emulsion or syrup. The preparation for parenteral administration may be a formulation for external application such as cream, ointment, gel, lotion, , Inhalants, aerosols, suppositories, and the like.

In the pharmaceutical composition for treating or improving skin conditions according to the present invention, when the pharmaceutical composition is used as a external preparation, it may be sterilized by a conventional method, such as an aqueous solution, a non-aqueous solvent, a suspending agent, As an appropriate external preparation for skin.

In the pharmaceutical composition for treating or improving skin conditions according to the present invention, the actual dosage or application amount of the pharmaceutical composition may be varied depending on the route of administration, the age, sex and weight of the patient, and the severity of the disease, And may be determined depending on the type of drug that is the component

According to a third embodiment,

The present invention provides a food for improving skin condition comprising chitosan as an active ingredient.

In the food for improving skin condition according to the present invention, the molecular weight of the chitosan is 1 KDa to 100 KDa.

In the food for improving skin condition according to the present invention, the chitosan is characterized by being 50% to 90% deacetylated.

In the food for improving skin condition according to the present invention, the food is characterized by having antimicrobial, anti-inflammatory and antioxidative properties.

In the food for improving skin condition according to the present invention, the food is characterized by having a skin whitening effect, a wrinkle improving effect, a skin moisturizing effect or a skin aging-preventing effect.

In the food for improving skin condition according to the present invention, the food may be in the form of a powder, a granule, a tablet, a capsule or a drink, and may be a candy, a chocolate, a drink, a gum, a tea, a vitamin complex,

Hereinafter, various embodiments are provided to facilitate understanding of the present invention. The following examples are provided to facilitate understanding of the invention and are not intended to limit the scope of the invention.

< Example >

Example  1. Various Deacetylation  Of chitosan library

To produce various chitosan libraries with deacetylation, 200 kg of chitin (DAC 4.1%) was charged to a 5 ton reactor, filled with 5 ton of 50% NaOH, and then stirred at 80 ° C with a 60 rpm impeller. The chitosan of the desired DAC was obtained by sampling each time, washed three times with water, and then adjusted to pH 7 with hydrochloric acid. The water was then drained and chitosan was recovered, dried and then ground. The deacetylation of the prepared chitosan was measured by toluidine blue analysis, and the results are shown in Table 1 below.

[Table 1]

Example  2. Preparation of chitosan library with various molecular weights

The chitosan prepared in Example 1 was classified into deacetylation ~50% (reaction time 3.5 hours), ~70% (reaction time 5.5 hours), and ~ 90% (reaction time 12 hours), chitosanase ) Was used to prepare chitosan having various molecular weights. 2 kg of chitosan was charged into a 50 L reactor, and water was filled up to 39 L, followed by stirring at 40 캜 and 180 rpm. Then, 0.7 L of 35% hydrochloric acid was added to adjust the pH to 4.8. Chitosanase 70,000 U (3.5 U / g) was added to the enzyme to decompose it, and a portion of the solution was recovered at each time point and the enzyme was immediately inactivated at 95 ° C for 30 minutes. Each solution was subjected to W4 paper to remove undifferentiated products, and the solution was lyophilized to prepare a lyophilized powder. The molecular weight of the prepared chitosan was measured by GPC analysis, and the results are shown in Table 2 below.

[Table 2]

< Experimental Example >

Experimental Example  1. Clinical skin irritation test of chitosan library according to the present invention

Chitosan library samples (DAC 50% (10, 20, 50, 100KDa), DAC 70% (5,10,20,50,100KDa), DAC 90% (3, 5,10,20,50,100 KDa) The skin irritation was evaluated according to the standard test method (SOP) of SkinMed Dermatological Research Institute by Skin Med. The subjects were selected from the group consisting of 33 adults (20 years old and 50 years old) who met the criteria for selection and exclusion of the test subjects (final dropout: none). The evaluation method was as follows: 24 hours after the patches and 48 hours The reaction that appeared later was visually evaluated. The results are shown in Table 3 below.

[Table 3]

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

A cosmetic composition for improving skin condition comprising chitosan as an active ingredient.
The method according to claim 1,
Wherein the molecular weight of the chitosan is from 1 KDa to 100 KDa.
The method according to claim 1,
Wherein the chitosan is 50% to 90% deacetylated.
The method according to claim 1,
Wherein the cosmetic composition has antibacterial, anti-inflammatory and antioxidative properties.
The method according to claim 1,
Wherein the cosmetic composition has a skin whitening effect, a wrinkle improving effect, a skin moisturizing effect or a skin aging-preventing effect.
A pharmaceutical composition for treatment or improvement of skin condition comprising chitosan as an active ingredient.
The method according to claim 6,
Wherein the molecular weight of the chitosan is from 1 KDa to 100 KDa.
The method according to claim 6,
Wherein the chitosan is 50% to 90% deacetylated.
The method according to claim 6,
Wherein said pharmaceutical composition is characterized by an antimicrobial, anti-inflammatory and antioxidant efficacy.
The method according to claim 6,
Wherein the pharmaceutical composition has a skin whitening effect, a wrinkle-improving effect, a skin moisturizing effect or a skin aging-preventing effect.