KR20190067483A - Method for producing red pepper paste using rice bran fermented solution - Google Patents
Method for producing red pepper paste using rice bran fermented solution Download PDFInfo
- Publication number
- KR20190067483A KR20190067483A KR1020170167457A KR20170167457A KR20190067483A KR 20190067483 A KR20190067483 A KR 20190067483A KR 1020170167457 A KR1020170167457 A KR 1020170167457A KR 20170167457 A KR20170167457 A KR 20170167457A KR 20190067483 A KR20190067483 A KR 20190067483A
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- South Korea
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- rice
- fermented
- rice bran
- red pepper
- fermenting
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Abstract
Description
본 발명은 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 제조한 미강 발효액을 이용한 고추장의 제조방법 및 상기 방법으로 제조된 미강 발효액을 이용한 장류에 관한 것이다.The present invention relates to a method for producing rice koji by mixing a mixture of water, rice gruel and salt in rice koji with a mixture of Zygosaccharomyces The present invention relates to a method for preparing a koji paste using fermented rice bran prepared by inoculating a fermented rice bran cultivated with a rouxii culture broth and a fermented soybean fermented by the above method.
고추장은 간장, 된장과 함께 예전부터 널리 애용되어 온 우리나라 고유의 전통 발효식품으로, 탄수화물에서 생성되는 당류의 단맛, 단백질에서 생성된 아미노산의 구수한 맛, 고춧가루의 매운맛, 그리고 소금의 짠맛 등이 잘 조화를 이루어 독특한 맛을 형성하는 복합 향신 조미료이다. 또한 된장과 간장에 비해 비타민 B1, B2, C 및 엽산(folic acid) 등이 다량 함유되어 있고, 미생물의 대사 및 발효작용으로 생성되는 유기산, 핵산, 알코올 및 색소 등의 미량성분이 맛, 향 및 색 등에 조화를 이루는 식품이다. 최근에는 소비자들의 식품 선택 시 식품의 관능적인 품질과 함께 기능성을 중시하는 경향으로 식생활 문화가 변화하고 있다. 그러한 변화를 반영하여 고추장에 다양한 식품을 첨가하여 풍미와 기능성을 동시에 향상시키려는 연구들이 보고되고 있다.Kochujang is a traditional Korean fermented food that has been widely used with soy sauce and soybean paste in the past. The sweetness of carbohydrate-derived sugars, the savory taste of amino acids produced by proteins, the hot spicy pepper powder, and the salty taste of salt To form a unique flavor. In addition, it contains a large amount of vitamin B1, B2, C and folic acid compared with soybean paste and soy sauce, and trace elements such as organic acid, nucleic acid, alcohol and pigment produced by metabolism and fermentation of microorganisms, It is food that harmonizes with color. Recently, the food culture of consumers is changing with the tendency to emphasize functionality along with sensual quality of food. Studies have been reported to improve flavor and functionality at the same time by adding various foods to kochujang reflecting such changes.
미강은 쌀을 도정한 후의 부산물로, 쌀은 원래 다른 곡류에 비해 당 함량이 높은 반면, 지방 및 단백질 함량이 낮고 특히 미네랄 및 비타민 함량이 매우 낮은 편이다. 그러나 최근 쌀에 함유된 쌀눈은 배유와 비교하여 양질의 단백질과 지방, 그리고 무기질 및 비타민 A, B, E 등이 함유된 좋은 영양원으로서 그 기능성을 인정받고 있으며, 아울러 쌀 기름은 올레산, 리놀레산 및 리놀렌산과 같은 불포화지방산을 다량 함유하고 있을 뿐 아니라 식물스테롤(phytosterol), γ-오리자놀(γ-oryzanol) 및 토코페롤 유도체와 같은 항암, 항고혈압, 항혈액 응고 및 항산화물질의 함량이 높아 기능성 신소재로써 각광을 받고 있다. 또한, 발아(현)미는 항노화 및 항고혈압성 생리활성물질로 알려진 GABA(γ-aminobutyric acid) 뿐만 아니라 필수아미노산인 류신, 이소류신 및 메티오닌 함량이 높고 식이성섬유소를 다량 함유하고 있어 최근 그 소비가 크게 증가하고 있다.Rice bran is a by-product after rice is cultivated. Rice has higher sugar content than other grains, but low in fat and protein content, especially in minerals and vitamins. However, recently, rice husk contained in rice has been recognized as a good nutrition source containing high quality protein, fat, minerals and vitamins A, B, and E as compared with that of endosperm. In addition, rice oil is oleic acid, linoleic acid and linolenic acid As well as phytosterol, γ-oryzanol and tocopherol derivatives, which are high in content of anticancer, anti-hypertension, anti-blood coagulation and antioxidant. . In addition, GABA (gamma-aminobutyric acid), which is known as anti-aging and antihypertensive physiologically active substance, has a high content of leucine, isoleucine and methionine, which are essential amino acids, and a large amount of dietary fiber, Has increased significantly.
한국등록특허 제1489999호에는 아스퍼질러스 오리재 CJ 1354 균주를 이용한 쌀 고추장의 제조방법이 개시되어 있고, 한국등록특허 제1062667호에는 김치유산균으로 발효한 저염분 및 고비타민 함유 고추장의 제조방법이 개시되어 있으나, 본 발명의 미강 발효액을 이용한 고추장의 제조방법과는 상이하다.Korean Patent No. 1489999 discloses a process for producing rice kochujang using Aspergillus oryzae CJ-1354. Korean Patent No. 1062667 discloses a process for preparing low-salt and high-vitamin-containing kochujang fermented with Kimchi lactic acid bacteria However, this method is different from the method for producing kochujang using the rice bran fermentation broth of the present invention.
본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 기능성 성분이 강화되면서 기호도가 우수한 고추장을 제조하기 위해, 기능성 균주 선정, 배합비 및 발효조건 등의 제조조건을 최적화하여, 영양성분이 강화되면서 기호도가 향상된 미강 발효액을 이용한 고추장의 제조방법을 제공하는 데 있다.SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to optimize manufacturing conditions such as selection of a functional strain, a blending ratio and a fermentation condition in order to manufacture a kochujang, The present invention provides a method of manufacturing a koji paste using the rice bran fermentation broth.
상기 과제를 해결하기 위해, 본 발명은 (a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계; (b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계; (c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및 (d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법을 제공한다.In order to solve the above problems, the present invention provides a method for producing rice koji, comprising the steps of: (a) watering rice, boiling it and cooling it, inoculating an Aspergillus oryzae strain, fermenting rice koji; (b) adding a mixture of water, rice gruel, and salt to the rice koji prepared in step (a) above to a mixture of Zygosaccharomyces rouxii ) and then fermenting the same to prepare a rice bran fermentation broth; (c) fermenting the mixture of rice ginseng, salt, red pepper powder and fermented rice flour, and the rice bran fermentation liquid prepared in step (b); And (d) mixing the fermented red pepper paste fermented in step (c) with red pepper powder, millet and starch. The present invention also provides a method of manufacturing a red pepper paste using the fermented rice bran.
또한, 본 발명은 상기 방법으로 제조된 미강 발효액을 이용한 장류를 제공한다.The present invention also provides a soybean paste using the rice bran fermentation broth prepared by the above method.
본 발명의 방법으로 제조된 고추장은 선정된 우수 효모로 제조된 미강 발효액을 이용함으로써 고추장의 영양성분 및 품질이 우수할 뿐만 아니라, 고추장의 감칠맛 및 구수한 맛이 증진되어 기호도가 우수한 고추장을 제공할 수 있다.Using the fermented rice bran prepared by the method of the present invention, the fermented rice bran prepared by the method of the present invention is excellent in the nutritional quality and quality of the fermented soybean paste, and enhances the richness and savory taste of the fermented soybean paste. have.
도 1은 균주 종류 및 발효기간에 따른 고추장 발효물의 수분 함량(%)을 비교한 그래프이다.
도 2는 균주 종류 및 발효기간에 따른 고추장 발효물의 pH를 비교한 그래프이다.
도 3은 균주 종류 및 발효기간에 따른 고추장 발효물의 아미노태 질소(㎎%) 함량을 비교한 그래프이다.
도 4는 균주 종류 및 발효기간에 따른 고추장 발효물의 산도(%)를 비교한 그래프이다.
도 1 내지 4의 숙성물 1: 지고사카로마이세스 룩시 SRCM100945 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물, 숙성물 5: 지고사카로마이세스 룩시 SRCM100949 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물, 숙성물 9: 지고사카로마이세스 룩시 SRCM100953 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물을 의미한다.FIG. 1 is a graph comparing the moisture content (%) of the fermented product of Kochujang with the type of strain and fermentation period.
FIG. 2 is a graph comparing the pH of fermented product of Kochujang with the type of strain and fermentation period.
FIG. 3 is a graph comparing amino acid nitrogen (㎎%) contents of the fermented product of Kochujang with the type of strain and fermentation period.
4 is a graph comparing the acidity (%) of the fermented product of Kochujang according to the strain type and fermentation period.
1 to 4: Fermented product of Kochujang prepared by adding rice bran fermentation broth using Jigoku Sakamisaisu Lucix SRCM100945 strain, fermented product 5: prepared by adding rice bran fermentation broth using Gigosakaromyces roxi SRCM100949 strain A fermented product of Kochujang, a fermented product 9: means a fermented product of Kochujang prepared by adding a rice bran fermentation broth using a strain of Gishi Sakaromai Kosirushi SRCM100953.
본 발명의 목적을 달성하기 위하여, 본 발명은 In order to achieve the object of the present invention,
(a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계;(a) immersing the rice in water, adding and cooling the rice, inoculating the Aspergillus oryzae strain and fermenting it to prepare rice koji;
(b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계;(b) adding a mixture of water, rice gruel, and salt to the rice koji prepared in step (a) above to a mixture of Zygosaccharomyces rouxii ) and then fermenting the same to prepare a rice bran fermentation broth;
(c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및(c) fermenting the mixture of rice ginseng, salt, red pepper powder and fermented rice flour, and the rice bran fermentation liquid prepared in step (b); And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법을 제공한다.(d) mixing the fermented red pepper paste fermented in step (c) with red pepper powder, millet and starch to prepare a koji paste using the fermented rice bran.
본 발명의 고추장의 제조방법에서, 상기 (a)단계의 쌀 코지는 바람직하게는 쌀을 4~6시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 28~32℃에서 44~52시간 동안 발효하여 제조할 수 있으며, 더욱 바람직하게는 쌀을 5시간 동안 수침한 후 121℃에서 10분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 30℃에서 48시간 동안 발효하여 제조할 수 있다. 상기와 같은 조건으로 쌀을 수침한 후 물빼기 및 증자하는 것이 적절히 증자됨으로써 미생물이 이용하는데 적합한 호화도와 과다한 증자에 의한 혐기적 조건 유발로 인한 잡균 오염 등을 방지할 수 있었고, 또한, 상기와 같은 발효과정을 통해 고추장 제조 시 발효를 촉진시킬 수 있는 쌀 코지로 제조할 수 있었다.The rice koji of the step (a) of the present invention is preferably prepared by soaking rice for 4 to 6 hours and then heating at 110 to 130 ° C for 8 to 12 minutes and cooling. As a result, The strain may be inoculated and fermented at 28 to 32 ° C for 44 to 52 hours. More preferably, the rice is soaked for 5 hours and then heated at 121 ° C for 10 minutes and cooled to produce Aspergillus oryzae strain Followed by inoculation and fermentation at 30 ° C for 48 hours. As a result, rice was soaked in water under the above-mentioned conditions and then water was drained and increased. Thus, it was possible to prevent sucking bacteria suitable for microorganisms and contamination of germs caused by anaerobic conditions caused by excessive growth. Through the fermentation process, it was possible to produce koji rice which can promote fermentation in the production of koji paste.
또한, 본 발명의 고추장의 제조방법에서, 상기 (b)단계의 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주는 지고사카로마이세스 룩시 SRCM100945 균주로, 상기 특정 균주를 이용하여 제조한 미강 발효액은 아미노태 질소 함량이 높으면서 풍미가 우수한 미강 발효액을 제조할 수 있었다.Further, in the method for producing koji puddings of the present invention, it is preferable that the step (b) is carried out in the presence of Zygosaccharomyces rouxii strain of the present invention was a strain of Gigasakaromai Kosirushi SRCM100945, and the rice bran fermentation broth prepared using the above strain was able to produce a fermentation broth having a high amino nitrogen content and excellent flavor.
또한, 본 발명의 고추장의 제조방법에서, 상기 (b)단계의 미강 발효액은 바람직하게는 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 제조할 수 있으며, 더욱 바람직하게는 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 제조할 수 있다. 상기와 같은 조건으로 제조된 미강 발효액을 첨가한 고추장은 식이섬유 함량이 높고, 감칠맛 및 풍미가 증진되는 이점이 있다. 또한, 상기와 같은 조건으로 발효하는 것이 미강 발효액을 보다 경제적으로 발효하면서 아미노태 질소 함량이 높고 풍미도 증진시킬 수 있었다.In addition, in the method for manufacturing a kochujang of the present invention, the rice bran fermentation liquid in step (b) is preferably prepared by mixing 450 to 550 mL of water, 180 to 240 g of rice bran, and 60 to 80 g of salt in 180 to 240 g of rice koji The mixture can be prepared by inoculating 0.8 to 1.2% (v / v) of a culture solution of Zygosaccharomyces rouxii strain to the mixture and then fermenting at 28 to 32 ° C for 6 to 8 days, To a mixture of 210 g of rice koji mixed with 500 mL of water, 210 g of rice bran and 70 g of salt was added a mixture of Zygosaccharomyces rouxii ) in a 1% (v / v) inoculum followed by fermentation at 30 ° C for 7 days. Kochujang added with the rice bran fermentation broth prepared under the above conditions has an advantage of high dietary fiber content, richness and flavor. In addition, fermenting the rice bran fermentation broth under the above conditions enabled the fermentation of the rice bran fermentation broth more economically, while increasing the amino nitrogen content and flavor.
또한, 본 발명의 고추장의 제조방법에서, 상기 (c)단계는 바람직하게는 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g, 메줏가루 70~90 g 및 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효할 수 있으며, 더욱 바람직하게는 증자쌀 80 g, 소금 40 g, 고춧가루 90 g, 메줏가루 80 g 및 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효할 수 있다. 상기와 같은 조건으로 발효하는 것이 이미 및 이취가 발생하지 않으면서 아미노태 질소 함량이 높아 품질이 우수한 고추장 제조에 적합한 조건으로 발효할 수 있었다.In step (c) of the present invention, preferably, the step (c) is carried out by mixing 70 to 90 g of rice ginseng, 35 to 45 g of salt, 80 to 100 g of red pepper powder, 70 to 90 g of fermented powder, 750 g, and then fermented at 28 to 32 ° C for 18 to 24 days. More preferably, 80 g of rice ginseng, 40 g of salt, 90 g of red pepper powder, 80 g of molasses, and 700 g of rice bran Followed by fermentation for 21 days at 30 ° C. Fermentation under the conditions described above could be carried out under conditions suitable for the production of high quality kochujang having a high content of amino nitrogen because no fermentation had already occurred and no offensive odor was generated.
본 발명의 미강 발효액을 이용한 고추장의 제조방법에서, 상기 (d)단계의 혼합은 바람직하게는 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합할 수 있으며, 더욱 바람직하게는 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합할 수 있다. 상기 (c) 및 (d)단계의 재료 및 배합비로 제조된 고추장은 재료들의 맛과 향이 잘 어우러져 고추장의 매운맛, 짠맛, 구수한 맛 및 감칠맛이 잘 조화되어 풍미가 우수하여 기호도를 향상시킬 수 있었다. 또한, 고춧가루 재료를 상기 (c) 및 (d)단계의 2단계에 걸쳐 배합함으로써 갈변에 의한 고추장 색상의 선호도 저하를 막을 수 있었다.In the method of manufacturing a koji paste using the rice bran fermentation broth according to the present invention, the mixing of the step (d) may be carried out by mixing 80 ~ 100 g of red pepper powder, 180 ~ 220 g of starch syrup and 45 ~ , More preferably 90 g of red pepper powder, 200 g of starch syrup and 50 g of crude oil can be mixed with the fermented product of Kochujang. The hot pepper paste prepared according to the materials and mixing ratio of the above (c) and (d) was well mixed with the taste and flavor of the ingredients, so that the hot, salty, savory and rich flavors of the hot pepper paste were well harmonized. In addition, by blending the red pepper powder material in two steps of (c) and (d), it was possible to prevent the degradation of the color preference of the red pepper paste caused by browning.
본 발명의 미강 발효액을 이용한 고추장의 제조방법은, 보다 구체적으로는The method for producing koji sauce using the rice bran fermentation broth of the present invention, more specifically,
(a) 쌀을 4~6시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 28~32℃에서 44~52시간 동안 발효하여 쌀 코지를 제조하는 단계;(a) After soaking the rice for 4 to 6 hours, it was heated at 110 to 130 ° C for 8 to 12 minutes and cooled to inoculate the Aspergillus oryzae strain, fermented at 28 to 32 ° C for 44 to 52 hours, ;
(b) 상기 (a)단계의 제조한 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 미강 발효액을 제조하는 단계;(b) 180 to 240 g of the rice koji prepared in the step (a) is mixed with 450 to 550 mL of water, 180 to 240 g of rice bran, and 60 to 80 g of salt to prepare a mixture of Zygosaccharomyces rouxii) a strain culture solution 0.8 ~ 1.2% (v / v ) and then inoculated to the fermentation at 28 ~ 32 ℃ for 6-8 days to prepare a rice bran fermentation broth;
(c) 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g 및 메줏가루 70~90 g과 상기 (b)단계의 제조한 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효하는 단계; 및(c) Mixing 70 to 90 g of rice ginseng, 35 to 45 g of salt, 80 to 100 g of red pepper powder and 70 to 90 g of fermented powder and 650 to 750 g of the fermented rice bran prepared in step (b) ≪ / RTI > for 18-24 days; And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합하는 단계를 포함할 수 있으며,(d) mixing 80 to 100 g of red pepper powder, 180 to 220 g of starch syrup and 45 to 55 g of fermented cornstarch into the fermented fermented product of step (c)
더욱 구체적으로는More specifically,
(a) 쌀을 5시간 동안 수침한 후 121℃에서 10분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 30℃에서 48시간 동안 발효하여 쌀 코지를 제조하는 단계;(a) submerging rice for 5 hours, heating at 121 ° C for 10 minutes and cooling, inoculating aspergillus oryzae strain and fermenting at 30 ° C for 48 hours to prepare rice koji;
(b) 상기 (a)단계의 제조한 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 미강 발효액을 제조하는 단계;(b) 210 g of the rice koji prepared in the above step (a) was mixed with 500 ml of water, 210 g of rice bran and 70 g of salt, and the culture solution of Zygosaccharomyces rouxii strain was mixed with 1% v / v) and then fermented at 30 ° C. for 7 days to prepare an undiluted fermented broth;
(c) 증자쌀 80 g, 소금 40 g, 고춧가루 90 g 및 메줏가루 80 g과 상기 (b)단계의 제조한 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효하는 단계; 및(c) fermenting 80 g of rice ginseng, 40 g of salt, 90 g of red pepper powder, 80 g of fermented rice flour and 700 g of fermented rice bran prepared in step (b), followed by fermentation at 30 ° C for 21 days; And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합하는 단계를 포함할 수 있다.(d) mixing 90 g of red pepper powder, 200 g of starch syrup and 50 g of crude starch into the fermented fermented product of Kochujang fermented in step (c).
본 발명은 또한, 상기 방법으로 제조된 미강 발효액을 이용한 고추장을 제공한다.The present invention also provides a koji paste using the fermented rice bran prepared by the above method.
이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, production examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples are illustrative of the present invention, and the content of the present invention is not limited to the following Production Examples and Examples.
제조예Manufacturing example 1. One. 미강Rice bran 발효액을 이용한 고추장 Kochujang using fermented liquid
(a) 쌀에 물을 첨가하여 5시간 동안 침지한 후 121℃에서 10분 동안 증자하고 30℃로 냉각한 밥에 아스퍼질러스 오리재(Aspergillus oryzae) 균주(105~6 CFU/ml) 0.01%를 접종하고 30℃ 및 습도 80% 조건에서 48시간 동안 발효하여 쌀 코지를 제조하였다.(a) Water was added to the rice, and the rice was immersed for 5 hours. Then, the rice was heated at 121 ° C for 10 minutes and cooled to 30 ° C. Aspergillus oryzae strains (10 5 to 6 CFU / ml) were inoculated and fermented at 30 ° C and 80% humidity for 48 hours to prepare rice koji.
(b) 상기 (a)단계의 제조한 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액(105~6 CFU/ml)을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 미강 발효액을 제조하였다.(b) 210 g of the rice koji prepared in the above step (a) is mixed with 500 ml of water, 210 g of rice bran and 70 g of salt, and the mixture of Zygosaccharomyces rouxii (10 5 - 6 CFU / ml) was inoculated at 1% (v / v) and fermented at 30 ℃ for 7 days.
(c) 쌀을 5시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자한 쌀 또는 상기 증자한 쌀을 분쇄한 호화 쌀가루 80 g과 소금 40 g, 고춧가루 90 g 및 메줏가루 80 g과 상기 (b)단계의 제조한 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효하였다.(c) After soaking the rice for 5 hours, 80 g of the rice rice grown in the temperature range of 110 to 130 ° C for 8 to 12 minutes or the rice rice added with the rice, 40 g of the salt, 90 g of red pepper powder, 80 g of the red pepper powder, 700 g of the rice bran fermentation solution prepared in the step (b) was mixed and fermented at 30 ° C for 21 days.
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합한 후, 80℃에서 15분 동안 살균하였다.(d) 90 g of red pepper powder, 200 g of starch syrup and 50 g of crude oil were mixed in the fermented fermented product of step (c) and sterilized at 80 ° C for 15 minutes.
1. 재료 및 방법1. Materials and Methods
1) 재료1) Materials
고추장 제조에 사용한 쌀, 대두, 고춧가루 등의 재료는 국내산(전북 순창군)을 사용하였다. 고추장을 발효하는데 사용하는 균주는 크게 탄수화물 분해능과 단백질 분해능이 우수한 아스퍼질러스 오리재(Aspergliius oryzae) 균주와 바실러스 서브틸리스(Bacillus subtilis) 균주이며, 곰팡이 균주는 충무발효 종균을 구입하여 사용하여 쌀코지를 제조하였고, 고초균인 바실러스 균주는 당사에서 메주발효에 사용되는 보관 균주를 사용하여 메줏가루를 제조하였다. 또한 고추장에 풍미를 주기 위한 효모로써 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주를 (재)발효미생물산업진흥원으로부터 분양받아서 사용하였다.The ingredients of rice, soybean, red pepper powder used in the manufacture of kochujang were domestic (Chunbuk Sunchang Army). The strains used for fermentation of kochujang are aspergillus oryzae, which are highly resistant to carbohydrate degradation and protein degradation oryzae strains and Bacillus subtilis strains. Fungus strains were purchased from Chungmu fermentation broth and used to produce rice koji. Bacillus strains of Bacillus subtilis were purchased from our company using a storage strain used for meju fermentation. Powder. In addition, as a yeast for imparting flavor to red pepper paste, Zygosaccharomyces rouxii strain was purchased from the Korea Fermentation Industry Promotion Agency.
2) 실험방법2) Experimental method
(가) 수분측정(A) Moisture measurement
시료 5 g씩 채취하여 적외선 수분측정기에서 수분을 측정하였으며, 3 반복 진행하였다.Five grams of each sample was sampled and moisture was measured by an infrared moisture meter.
(나) pH 및 적정 산도 측정 (B) Measurement of pH and titratable acidity
시료를 채취하여 믹서기로 마쇄한 후 무게 5 g을 취하고 증류수를 가하여 50 mL로 맞춘 후 이를 충분히 혼합한 후 pH 미터(Mettler Toledo Gmbh, Switzerland) 를 이용하여 측정하였고, 적정 산도는 0.1N-NaOH를 가하여 pH 8.4가 될 때까지 적정하였다.The sample was collected and ground with a blender, and weighed 5 g. Distilled water was added to make 50 mL. The mixture was thoroughly mixed and measured using a pH meter (Mettler Toledo Gmbh, Switzerland). Titratable acidity was 0.1 N NaOH And the pH was adjusted to 8.4.
(다) 아미노태 질소(amino-type nitrogen) 측정(C) Amino-type nitrogen measurement
아미노태 질소는 Formol법으로 측정하였다. 시료 1 g을 비커에 취하고 증류수 50 mL를 가하고 240초 동안 진탕 혼합하여 충분히 용해한 다음 0.1N-NaOH 용액으로 적정하여 pH 8.4가 되도록 적정하였다. 여기에 중성 포르말린(formalin) 20 mL를 가하고 20초 동안 진탕 혼합한 뒤 다시 0.1N-NaOH 용액으로 pH 8.4가 되도록 중화 적정하였다.Amino nitrogen was measured by the Formol method. 1 g of the sample was taken in a beaker, 50 mL of distilled water was added, and the mixture was thoroughly dissolved by shaking for 240 seconds. The solution was titrated with 0.1 N NaOH solution to a pH of 8.4. To this was added 20 mL of formalin, shaken for 20 seconds, and neutralized to pH 8.4 with 0.1 N NaOH solution.
(라) 관능검사(D) Sensory evaluation
본 발명의 고추장의 출시가능성을 최종 점검하기 위해 관능특성을 분석하였다. 보다 상세하게는 1단계의 풍미 기호도 분석은 연구원을 대상으로 간이 실험을 실시하고 대량 pilot-scale로 가기 위한 기초자료로써 활용하였다. 2단계 풍미 기호도 분석(pilot-scale)은 평점법에서 항목척도를 사용하여 5점 척도로 평가토록 하며 농업회사법인 순창장류(주)의 전직원을 대상으로 짠맛과 감칠맛 농도별 미맹테스트를 진행한 후 선발된 인원인 12명을 대상으로 실시하였다.The sensory characteristics of the present invention were analyzed in order to finally check the possibility of launching kochujang. More specifically, the first-stage flavor preference analysis was used as a basic data to conduct a pilot experiment on researchers and to go to a large pilot-scale. The pilot scale of the second stage was evaluated by using the item scale in the rating method and evaluated on a scale of 5 points. All the employees of the agricultural company SUNCHANG JOURNAL Co., Ltd. were tested for their salty taste and richness concentration The selected 12 persons were selected.
(마) 식이섬유 측정(E) Dietary fiber measurement
고추장 제조 후 1차적으로 전북대학교 바이오식품소재개발 및 산업화연구센터에 의뢰하여 식이섬유 함량을 분석하도록 하고, 최종적으로 과제 제출용으로 공인분석기관에 의뢰하여 식이섬유량에 대한 분석결과를 확보하였다.After the preparation of kochujang, we first asked the bio-food material development and industrialization research center of Chonbuk National University to analyze the dietary fiber content. Finally, the analytical result of dietary fiber amount was secured by the authorized analytical agency for submission.
실시예Example 1. One. 미강Rice bran 발효액 제조를 위한 균주 선발 Selection of strains for fermentation
실험 균주는 하기 표 1과 같이 (재)발효미생물산업진흥원으로부터 균주를 제공받아 사용하였으며, 미강 혼합물 제조 후 YM 배지에서 액상 배양한 효모 배양액은 0.3%씩 접종하여 30℃에서 7일 동안 발효를 진행하였다. 풍미 관능검사와 아미노태 질소(AN) 분석치를 기준으로 1차로 3종의 효모를 분리하였다. 균주 선발을 위한 관능검사의 경우 간이실험으로 진행하였으며 연구원을 대상으로 직접 향을 맡아보고 1부터 7까지(1점: 매우 좋지않음, 7점: 매우 좋음)의 점수를 주도록 하였으며 실험결과는 표 2와 같이 나타내었다.The test strains were used as the strains from the Korea Institute of Fermentation and Biotechnology (KFDA) as shown in Table 1. The fermentation was carried out at 30 ° C for 7 days by inoculating 0.3% of the yeast culture liquid in the YM medium after the preparation of the rice bran mixture Respectively. Three kinds of yeast were isolated on the basis of flavor sensory test and amino acid analysis (AN). In the case of the sensory test for the strain selection, the experiment proceeded to a simple experiment and the researchers were asked to give a score of 1 to 7 (1 point: not very good, 7 points: very good) Respectively.
그 결과, 상대적으로 1번, 3번, 5번, 9번, 10번 균주의 풍미 선호도가 우수한 것으로 나타났으며, 아미노태 질소 함량은 1번, 5번 및 9번 균주에서 높게 나타났다. 따라서 관능 선호도가 높으면서 아미노태 질소 수치가 상대적으로 높게 나타난 1번, 5번, 9번 균주인 지고사카로마이세스 룩시 SRCM100945, 지고사카로마이세스 룩시 SRCM100949, 지고사카로마이세스 룩시 SRCM100953의 3가지 균주를 고추장 제조 비교 실험에 사용하도록 하였다.As a result, the flavor preference of strains 1, 3, 5, 9, and 10 was relatively good, and amino acid nitrogen content was high in strains 1, 5, and 9. Therefore, there are three types of strains: Glycyrrhiza majoris Lucixi SRCM100945, Gigusaccharomyces lucilus SRCM100949, and Gigusakaromiasu Lucixi SRCM100953, which have higher amino acid nitrogen levels and higher sensory preference. The strains were used for comparative experiments of kochujang production.
실시예Example 2. 발효조건에 따른 2. Depending on fermentation conditions 미강Rice bran 발효액 최적 발효조건 설계 Design of optimal fermentation conditions for fermentation broth
최적 발효 조건의 설계를 위해 선별된 3종의 발효 균주를 사용한 미강 발효액을 30℃에서 7일과 14일간 발효를 진행하여 품질 특성을 분석해 보았다. 미강 발효액은 발효기간을 달리하여 제조한 후 pH, 아미노태 질소 및 관능 풍미를 분석하였으며, 그 결과는 표 3에 나타내었다.For the design of optimal fermentation conditions, the fermentation of rice bran fermentation broth using 3 selected fermentation strains was carried out at 30 ℃ for 7 days and 14 days. The fermented rice bran was prepared by varying the fermentation period, and pH, amino nitrogen and sensory flavor were analyzed. The results are shown in Table 3.
pH는 7일 발효 후 5.6~5.8 수준이었으며 14일 발효시 5.4~6.0을 나타내었다. 9번 균주를 이용한 미강 발효액의 경우 14일 발효시 항온항습기 보관 중 표면 효모가 관찰되었으며 좋지 않은 풍미가 나타났다. 1번과 5번 균주를 이용한 미강 발효액의 경우 7일 발효와 14일 발효의 풍미 차이가 크지는 않았으나, 발효가 진행됨에 따라 물성이 매우 묽은 것으로 나타나 차후 소재로 사용시 물성 품질의 조정에 애로 사항이 발생할 것으로 판단되었다. 아미노태 질소(AN) 함량은 발효기간이 늘어남에 따라 일부 증가하였으나 원료로부터 오는 함량에 한계가 있어 차이가 크지 않은 것으로 판단된다. 이상과 같이 발효기간의 연장이 대량 생산시 상기와 같은 문제점이 발생하는 바, 효모를 이용한 미강 발효액의 최적 발효조건으로는 30℃에서 7일간 발효하는 것이 품질 표준화 제품 생산에 유리할 것으로 판단된다.The pH was 5.6 ~ 5.8 after 7 days fermentation and 5.4 ~ 6.0 at 14 days fermentation. In the case of rice bran fermented with strain No. 9, surface yeast was observed during fermentation for 14 days during storage at constant temperature and humidity. Although the difference in flavor between 7 and 14 days fermentation was not large in the rice bran fermentation broth using strains 1 and 5, the fermentation showed that the physical properties were very dilute as the fermentation progressed. . Amino - nitrogen (AN) content increased with fermentation period but it was not significant because there was a limit of content from raw materials. As described above, when the fermentation period is prolonged in mass production, the above problems occur. As the optimum fermentation conditions of the rice bran fermentation broth using yeast, fermentation at 30 ° C for 7 days is considered to be advantageous for production of quality standardized products.
(7점 척도법)Sensory test
(7 point scaling method)
실시예Example 3. 3. 미강Rice bran 발효액을 최적화할 수 있는 우량 균주의 선발(2차) Selection of Superior Strain to Optimize Fermentation Liquor (2nd)
1차 선발과정을 거친 3종의 균주를 이용한 미강 발효액으로 고추장을 제조한 후 품질을 비교 분석함으로써 최종 1종의 균주를 선발코자 하였다. 고추장 발효는 3주간 실시하였으며, 이는 발효를 오래 하더라도 아미노태 질소 수치의 급격한 상승은 없었으며, 오히려 과 발효시 pH의 하락으로 이미나 이취가 생기는 문제가 있어 3주간 발효하였다.We selected the final strains by comparing the quality of Kochujang prepared with rice fermented broth using three strains selected through the first selection process. Kochujang fermentation was carried out for 3 weeks, and the fermentation did not show a rapid increase in amino nitrogen level even after long fermentation, but fermentation occurred for 3 weeks due to the problem of imina odor due to decrease in pH during fermentation.
균주 종류 및 발효기간에 따른 고추장의 수분, pH, AN, 적정 산도를 분석한 결과는 도 1 내지 4와 같다. 수분 함량은 고추장을 숙성하는 과정에서 약 3% 가량 증가하는 것으로 나타났으며(도 1), pH는 초기에 비해 하락하여 5.5~5.6 수준을 나타내었다(도 2). 이때 pH의 하락 패턴은 균주별 차이를 보였는데, 1번 균주를 이용한 1번 고추장 발효물의 경우 단계별로 떨어졌고, 5번, 9번 균주를 이용한 5번 고추장 발효물 및 9번 고추장 발효물의 경우 발효 초기에 급격히 떨어지다가 일정수준으로 유지하는 것으로 나타났다. 아미노태 질소 함량은 발효기간이 증가함에 따라 상승하여 3주 발효 후 200 mg% 이상을 나타내었고, 1번 균주를 이용한 고추장 발효물이 가장 높게 나타났다(도 3). 발효기간에 따른 고추장 발효물 적정 산도의 변화는 발효 초기에 증가하였다가 시간이 갈수록 감소하는 경향이 나타났으며, 1번 균주를 이용한 고추장 발효물이 산도가 상대적으로 높아 풍미 유기산 등의 함량이 높은 것으로 판단할 수 있었다(도 4). 본 실험결과를 토대로 1번 균주를 이용하는 것이 고품질의 고추장 제조에 가장 적합할 것으로 판단되어, 1번 균주인 지고사카로마이세스 룩시 SRCM100945 균주를 고추장에 첨가되는 미강 발효액 제조용 효모균으로 최종 선발하였다.The results of analysis of moisture, pH, AN and titratable acidity of kochujang according to strain type and fermentation period are shown in Figs. 1 to 4. Moisture content increased about 3% in the process of aging of kochujang (Fig. 1), and the pH dropped from 5.5 to 5.6 (Fig. 2). At this time, the drop pattern of pH showed difference by strains. In case of 1st fermented Kochujang fermented with No. 1 strain, It was found that it fell rapidly at the beginning and kept at a certain level. Amino nitrogen contents increased with increasing fermentation period and showed more than 200 mg% after 3 weeks fermentation, and fermented Kochujang fermented with 1 st strain was the highest (Fig. 3). Changes in titratable acidity of fermented Kochujang fermented during fermentation period increased at the early stage of fermentation and gradually decreased with time. The fermented product of Kochujang fermented with strain No. 1 had relatively high acidity, (Fig. 4). Based on the results of this experiment, it was concluded that the use of the strain No. 1 is most suitable for producing high quality kochujang. Thus, the strain Gosakaromyces Lucix SRCM100945, the first strain, was finally selected as a yeast strain for the preparation of rice bran fermented with koji.
실시예Example 4. 4. 미강Rice bran 발효액을 이용한 고추장 관능검사 Sensory Evaluation of Gochujang Using Fermented Liquid
실시예 3에서 선정된 지고사카로마이세스 룩시 SRCM100945 균주로 제조된 미강 발효액을 이용한 고추장과 상기 미강 발효액을 사용하지 않고 제조한 고추장(대조구)을 가지고 관능검사를 실시한 결과는 하기 표 4와 같다.The results of the sensory evaluation of Kochujang prepared with the rice germicidal fermented SRCM100945 strain selected in Example 3 and the Kochujang prepared without using the above fermented rice bran (control) are shown in Table 4 below.
그 결과, 대조구 고추장에 비해 본 발명의 제조예 1의 고추장은 감칠맛 및 풍미가 우수하여 고부가가치 셀링 포인트(selling point)를 갖는 제품으로 판단된다.As a result, the product of Preparation Example 1 of the present invention was superior to the control kochujang in that it had a rich flavor and flavor, and thus it was judged to be a product having a high value-added selling point.
실시예Example 5. 고추장 시제품의 가스 발생 유무 5. Whether gas is generated from the crude product of kochujang
제조예 1의 고추장 시제품의 유통 안정성 여부를 판단하기 위해, 고추장 제품의 가스 생성 여부를 확인하였다. 미강 발효액 제조에 사용한 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주는 발효 시 가스를 발생시키는 균주로 알려져 있다. 제조예 1의 고추장과 제조예 1의 방법으로 제조하되 미강 발효액 무첨가 고추장(비접종)의 가스 발생 여부를 확인하였다. 제조된 고추장을 각각 50 g씩 PE(Polyethlene) 필름에 담아 밀봉한 후 30℃ 인큐베이터에서 일정기간 동안 팽창 부피를 체크하였다. 부피체크는 메스실린더에 물을 채운 후 샘플봉지에 넣어 늘어난 부피로 가스 발생 여부를 체크하였다.In order to determine the distribution stability of the crude product of the preparation example 1, it was confirmed whether or not the product of the production of kochujang produced gas. The fermentation broth of Zygosaccharomyces rouxii ) is known as a strain which generates gas upon fermentation. The kochujang prepared in Preparation Example 1 and the method in Production Example 1 were used to confirm whether or not the gas generation of the kochujang (non-inoculated) without added rice bran fermentation solution was confirmed. The prepared kochujangs were sealed in PE (Polyethlene) film in an amount of 50 g each, sealed in a 30 ° C incubator, and the expansion volume was checked for a predetermined period. The volume check was performed by filling the measuring cylinder with water and putting it in a sample bag to check whether the gas was generated in an increased volume.
그 결과, 미강 발효액 무첨가 고추장과 미강 발효액 첨가 고추장 모두에서 가스발생은 관찰되지 않았다.As a result, no gas generation was observed in both of the kochujang without added rice bran and the added kochujang without bran.
N.D: 검출되지 않음N.D: Not detected
실시예Example 6. 고추장 시제품의 식이섬유 함량 분석 6. Analysis of Dietary Fiber Contents of Kochujang Prototype
제조예 1의 고추장과 제조예 1의 방법으로 제조하되 미강 발효액 무첨가 고추장(대조구)의 식이섬유 함량을 분석한 결과는 하기 표 6과 같다. 그 결과, 미강 발효액을 사용한 고추장의 식이섬유 함량이 약 2.5 g 높은 7.72 g으로 높게 나타났다.The dietary fiber contents of the kochujang prepared in Preparation Example 1 and the method of Preparation Example 1 were analyzed and the content of dietary fiber in the kochujang containing no rice germ fermentation broth (control) was analyzed. As a result, the content of dietary fiber of Kochujang using rice bran fermented liquid was as high as 7.72 g which is about 2.5 g higher.
실시예Example 7. 고추장 재료 배합비에 따른 관능검사 7. Sensory evaluation according to ingredients ratio of kochujang
상기 제조예 1의 방법으로 제조된 고추장, 상기 제조예 1의 방법으로 고추장을 제조하되 미강 발효액 및 고추장 재료 배합비를 달리하여 제조된 고추장(비교예 1 내지 4)을 가지고 5점 척도법으로 관능검사를 실시하였다.The kochujang prepared according to the method of Preparation Example 1 and the kochujang prepared according to the method of Preparation Example 1 were subjected to the sensory evaluation by the 5 point scale method using the koji paste prepared according to the mixing ratio of the raw rice bran meal and the koji paste (Comparative Examples 1 to 4) Respectively.
그 결과, 다른 재료 및 배합비로 제조된 비교예들의 고추장에 비해 제조예 1의 고추장이 외관, 감칠맛 및 향에 대한 기호도에서 더욱 선호하는 것을 확인할 수 있었고, 전반적인 기호도에서도 4.4점의 높은 점수를 나타냄을 확인할 수 있었다.As a result, it was confirmed that the kochujang prepared in Preparation Example 1 was more preferable in terms of appearance, flavor and aroma than the comparative examples prepared in other materials and blending ratio, and the score of 4.4 was higher than the overall likelihood I could confirm.
Claims (4)
(b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계;
(c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법.(a) immersing the rice in water, adding and cooling the rice, inoculating the Aspergillus oryzae strain and fermenting it to prepare rice koji;
(b) adding a mixture of water, rice gruel, and salt to the rice koji prepared in step (a) above to a mixture of Zygosaccharomyces rouxii ) and then fermenting the same to prepare a rice bran fermentation broth;
(c) fermenting the mixture of rice ginseng, salt, red pepper powder and fermented rice flour, and the rice bran fermentation liquid prepared in step (b); And
(d) mixing the fermented red pepper paste fermented in step (c) with red pepper powder, millet and starch.
(a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계;
(b) 상기 (a)단계의 제조한 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 28~32℃에서 6~8일 동안 발효하여 미강 발효액을 제조하는 단계;
(c) 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g 및 메줏가루 70~90 g과 상기 (b)단계의 제조한 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효하는 단계; 및
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법.The method according to claim 1,
(a) immersing the rice in water, adding and cooling the rice, inoculating the Aspergillus oryzae strain and fermenting it to prepare rice koji;
(b) 180 to 240 g of the rice koji prepared in the step (a) is mixed with 450 to 550 mL of water, 180 to 240 g of rice bran, and 60 to 80 g of salt to obtain a mixture of Zygosaccharomyces rouxii ) and then fermenting at 28 to 32 ° C for 6 to 8 days to prepare a rice bran fermentation broth;
(c) Mixing 70 to 90 g of rice ginseng, 35 to 45 g of salt, 80 to 100 g of red pepper powder and 70 to 90 g of fermented powder and 650 to 750 g of the fermented rice bran prepared in step (b) ≪ / RTI > for 18-24 days; And
(d) mixing 80 ~ 100 g of red pepper powder, 180 ~ 220 g of starch syrup and 45 ~ 55 g of fermented cornstarch in the fermented fermented product of step (c). ≪ / RTI >
(a) 쌀을 4~6시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 28~32℃에서 44~52시간 동안 발효하여 쌀 코지를 제조하는 단계;
(b) 상기 (a)단계의 제조한 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 미강 발효액을 제조하는 단계;
(c) 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g 및 메줏가루 70~90 g과 상기 (b)단계의 제조한 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효하는 단계; 및
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법.3. The method of claim 2,
(a) After soaking the rice for 4 to 6 hours, it was heated at 110 to 130 ° C for 8 to 12 minutes and cooled to inoculate the Aspergillus oryzae strain, fermented at 28 to 32 ° C for 44 to 52 hours, ;
(b) 180 to 240 g of the rice koji prepared in the step (a) is mixed with 450 to 550 mL of water, 180 to 240 g of rice bran, and 60 to 80 g of salt to prepare a mixture of Zygosaccharomyces rouxii) a strain culture solution 0.8 ~ 1.2% (v / v ) and then inoculated to the fermentation at 28 ~ 32 ℃ for 6-8 days to prepare a rice bran fermentation broth;
(c) Mixing 70 to 90 g of rice ginseng, 35 to 45 g of salt, 80 to 100 g of red pepper powder and 70 to 90 g of fermented powder and 650 to 750 g of the fermented rice bran prepared in step (b) ≪ / RTI > for 18-24 days; And
(d) mixing 80 ~ 100 g of red pepper powder, 180 ~ 220 g of starch syrup and 45 ~ 55 g of fermented cornstarch in the fermented fermented product of step (c). ≪ / RTI >
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2002330723A (en) * | 2001-02-28 | 2002-11-19 | Samjo Celltech Ltd | Noodle using soybean embryo and method for producing the same and hishios produced by using the same |
KR20050050750A (en) * | 2003-11-26 | 2005-06-01 | 김무겸 | A manufacturing method of fermented soybean sauce etc. by rice bran |
KR101489999B1 (en) * | 2012-10-31 | 2015-02-04 | 씨제이제일제당 (주) | Novel Aspergillus oryzae CJ 1354 isolated from the Korean traditional meju and the method of rice hot pepper paste using this fungi and the rice hot pepper paste from manufactured thereof |
KR20160130536A (en) * | 2015-05-04 | 2016-11-14 | 위현숙 | Manufacturing method of toenjang and soy source included rice bran |
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JP2002330723A (en) * | 2001-02-28 | 2002-11-19 | Samjo Celltech Ltd | Noodle using soybean embryo and method for producing the same and hishios produced by using the same |
KR20050050750A (en) * | 2003-11-26 | 2005-06-01 | 김무겸 | A manufacturing method of fermented soybean sauce etc. by rice bran |
KR101489999B1 (en) * | 2012-10-31 | 2015-02-04 | 씨제이제일제당 (주) | Novel Aspergillus oryzae CJ 1354 isolated from the Korean traditional meju and the method of rice hot pepper paste using this fungi and the rice hot pepper paste from manufactured thereof |
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