KR102209226B1 - Method for producing red pepper paste using rice bran fermented solution - Google Patents
Method for producing red pepper paste using rice bran fermented solution Download PDFInfo
- Publication number
- KR102209226B1 KR102209226B1 KR1020170167457A KR20170167457A KR102209226B1 KR 102209226 B1 KR102209226 B1 KR 102209226B1 KR 1020170167457 A KR1020170167457 A KR 1020170167457A KR 20170167457 A KR20170167457 A KR 20170167457A KR 102209226 B1 KR102209226 B1 KR 102209226B1
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- KR
- South Korea
- Prior art keywords
- rice
- rice bran
- red pepper
- fermented
- gochujang
- Prior art date
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- C12R2001/69—Aspergillus oryzae
Abstract
본 발명은 (a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계; (b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계; (c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및 (d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법 및 상기 방법으로 제조된 미강 발효액을 이용한 장류에 관한 것이다.The present invention comprises the steps of: (a) soaking rice, then steaming, cooling, inoculating the Aspergillus duckweed strain, and fermenting it to prepare rice koji; (b) Zygosaccharomyces lux ( Zygosaccharomyces) in a mixture of water, rice bran, and salt in the rice koji prepared in step (a) rouxii ) preparing a rice bran fermentation broth by fermenting after inoculating the strain culture broth; (c) fermenting after mixing the steamed rice, salt, red pepper powder, and soybean meal with the fermented rice bran fermentation broth prepared in step (b); And (d) mixing red pepper powder, starch syrup, and jocheong with the fermented gochujang fermented product of step (c), and a method for producing red pepper paste using rice bran fermentation broth, and the rice bran fermentation broth prepared by the method. It is about paste using.
Description
본 발명은 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 제조한 미강 발효액을 이용한 고추장의 제조방법 및 상기 방법으로 제조된 미강 발효액을 이용한 장류에 관한 것이다.The present invention is Zygosaccharomyces lux in a mixture of water, rice bran, and salt in rice koji. rouxii ) It relates to a method for producing gochujang using a rice bran fermentation broth prepared by fermentation after inoculating a strain culture broth, and to a paste using the rice bran fermentation broth prepared by the above method.
고추장은 간장, 된장과 함께 예전부터 널리 애용되어 온 우리나라 고유의 전통 발효식품으로, 탄수화물에서 생성되는 당류의 단맛, 단백질에서 생성된 아미노산의 구수한 맛, 고춧가루의 매운맛, 그리고 소금의 짠맛 등이 잘 조화를 이루어 독특한 맛을 형성하는 복합 향신 조미료이다. 또한 된장과 간장에 비해 비타민 B1, B2, C 및 엽산(folic acid) 등이 다량 함유되어 있고, 미생물의 대사 및 발효작용으로 생성되는 유기산, 핵산, 알코올 및 색소 등의 미량성분이 맛, 향 및 색 등에 조화를 이루는 식품이다. 최근에는 소비자들의 식품 선택 시 식품의 관능적인 품질과 함께 기능성을 중시하는 경향으로 식생활 문화가 변화하고 있다. 그러한 변화를 반영하여 고추장에 다양한 식품을 첨가하여 풍미와 기능성을 동시에 향상시키려는 연구들이 보고되고 있다.Gochujang is a traditional fermented food unique to Korea that has been widely used in the past along with soy sauce and miso. It blends well with the sweet taste of sugars produced from carbohydrates, the savory taste of amino acids produced from protein, the spicy taste of red pepper powder, and the salty taste of salt. It is a complex flavoring seasoning that forms a unique taste. In addition, compared to soybean paste and soy sauce, vitamins B1, B2, C and folic acid are contained in a large amount, and trace components such as organic acids, nucleic acids, alcohols, and pigments produced by the metabolism and fermentation of microorganisms are It is a food that harmonizes with color, etc. In recent years, when consumers choose food, the dietary culture is changing with a tendency to value the sensual quality and functionality of the food. Reflecting such changes, studies have been reported to simultaneously improve flavor and functionality by adding various foods to gochujang.
미강은 쌀을 도정한 후의 부산물로, 쌀은 원래 다른 곡류에 비해 당 함량이 높은 반면, 지방 및 단백질 함량이 낮고 특히 미네랄 및 비타민 함량이 매우 낮은 편이다. 그러나 최근 쌀에 함유된 쌀눈은 배유와 비교하여 양질의 단백질과 지방, 그리고 무기질 및 비타민 A, B, E 등이 함유된 좋은 영양원으로서 그 기능성을 인정받고 있으며, 아울러 쌀 기름은 올레산, 리놀레산 및 리놀렌산과 같은 불포화지방산을 다량 함유하고 있을 뿐 아니라 식물스테롤(phytosterol), γ-오리자놀(γ-oryzanol) 및 토코페롤 유도체와 같은 항암, 항고혈압, 항혈액 응고 및 항산화물질의 함량이 높아 기능성 신소재로써 각광을 받고 있다. 또한, 발아(현)미는 항노화 및 항고혈압성 생리활성물질로 알려진 GABA(γ-aminobutyric acid) 뿐만 아니라 필수아미노산인 류신, 이소류신 및 메티오닌 함량이 높고 식이성섬유소를 다량 함유하고 있어 최근 그 소비가 크게 증가하고 있다.Rice bran is a by-product of rice milling. Rice has a high sugar content compared to other grains, but has a low fat and protein content, and especially minerals and vitamins. However, rice sprouts contained in rice are recently recognized for their functionality as a good nutrient source containing high-quality protein, fat, and minerals and vitamins A, B, E, etc. compared to endosperm, and rice oil is also known as oleic acid, linoleic acid and linolenic acid. In addition to containing a large amount of unsaturated fatty acids such as phytosterol, γ-oryzanol and tocopherol derivatives such as anti-cancer, anti-hypertension, anti-blood coagulation and antioxidant content, it has been in the spotlight as a new functional material. Receiving. In addition, germinated (current) rice has high content of essential amino acids leucine, isoleucine and methionine, as well as GABA (γ-aminobutyric acid), which is known as an anti-aging and antihypertensive physiologically active substance, and contains a large amount of dietary fiber. It is increasing significantly.
한국등록특허 제1489999호에는 아스퍼질러스 오리재 CJ 1354 균주를 이용한 쌀 고추장의 제조방법이 개시되어 있고, 한국등록특허 제1062667호에는 김치유산균으로 발효한 저염분 및 고비타민 함유 고추장의 제조방법이 개시되어 있으나, 본 발명의 미강 발효액을 이용한 고추장의 제조방법과는 상이하다.Korean Patent No. 1489999 discloses a method of manufacturing rice gochujang using Aspergillus duckweed CJ 1354 strain, and Korean Patent No. 1062667 discloses a method for preparing red pepper paste containing low salt and high vitamin fermented with kimchi lactic acid bacteria. Although disclosed, it is different from the manufacturing method of red pepper paste using the rice bran fermentation broth of the present invention.
본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 기능성 성분이 강화되면서 기호도가 우수한 고추장을 제조하기 위해, 기능성 균주 선정, 배합비 및 발효조건 등의 제조조건을 최적화하여, 영양성분이 강화되면서 기호도가 향상된 미강 발효액을 이용한 고추장의 제조방법을 제공하는 데 있다.The present invention was conceived by the above requirements, and an object of the present invention is to optimize production conditions such as selection of functional strains, mixing ratios and fermentation conditions in order to manufacture red pepper paste having excellent palatability while enhancing functional ingredients, The purpose of this is to provide a method of manufacturing red pepper paste using fermented rice bran fermented broth with improved palatability while strengthening.
상기 과제를 해결하기 위해, 본 발명은 (a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계; (b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계; (c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및 (d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of: (a) immersing rice, increasing it, cooling it, inoculating the Aspergillus duckweed strain, and fermenting it to prepare rice koji; (b) Zygosaccharomyces lux ( Zygosaccharomyces) in a mixture of water, rice bran, and salt in the rice koji prepared in step (a) rouxii ) preparing a rice bran fermentation broth by fermenting after inoculating the strain culture broth; (c) fermenting after mixing the steamed rice, salt, red pepper powder, and soybean meal with the fermented rice bran fermentation broth prepared in step (b); And (d) mixing red pepper powder, starch syrup, and jocheong with the fermented gochujang fermented product of step (c). It provides a method for producing red pepper paste using a rice bran fermentation broth.
또한, 본 발명은 상기 방법으로 제조된 미강 발효액을 이용한 장류를 제공한다.In addition, the present invention provides a paste using the rice bran fermentation broth prepared by the above method.
본 발명의 방법으로 제조된 고추장은 선정된 우수 효모로 제조된 미강 발효액을 이용함으로써 고추장의 영양성분 및 품질이 우수할 뿐만 아니라, 고추장의 감칠맛 및 구수한 맛이 증진되어 기호도가 우수한 고추장을 제공할 수 있다.Gochujang prepared by the method of the present invention is not only excellent in nutritional components and quality, but also the umami and savory taste of gochujang by using the rice bran fermented broth made from selected excellent yeast, so that the red pepper paste with excellent preference can be provided. have.
도 1은 균주 종류 및 발효기간에 따른 고추장 발효물의 수분 함량(%)을 비교한 그래프이다.
도 2는 균주 종류 및 발효기간에 따른 고추장 발효물의 pH를 비교한 그래프이다.
도 3은 균주 종류 및 발효기간에 따른 고추장 발효물의 아미노태 질소(㎎%) 함량을 비교한 그래프이다.
도 4는 균주 종류 및 발효기간에 따른 고추장 발효물의 산도(%)를 비교한 그래프이다.
도 1 내지 4의 숙성물 1: 지고사카로마이세스 룩시 SRCM100945 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물, 숙성물 5: 지고사카로마이세스 룩시 SRCM100949 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물, 숙성물 9: 지고사카로마이세스 룩시 SRCM100953 균주를 이용한 미강 발효액을 첨가하여 제조한 고추장 발효물을 의미한다.1 is a graph comparing the moisture content (%) of fermented red pepper paste according to strain type and fermentation period.
2 is a graph comparing the pH of fermented red pepper paste according to strain type and fermentation period.
3 is a graph comparing the amino nitrogen (mg%) content of fermented pepper paste according to the strain type and fermentation period.
4 is a graph comparing the acidity (%) of fermented red pepper paste according to strain type and fermentation period.
Aged products of FIGS. 1 to 4: Fermented red pepper paste prepared by adding fermented rice bran fermentation broth using the strain of Saccharomyces lux SRCM100945, aging product 5: Manufactured by adding fermented rice bran fermentation broth using the Saccharomyces lux SRCM100949 strain One fermented product of red pepper paste, aged product 9: refers to a fermented product of red pepper paste prepared by adding fermented rice bran fermented liquid using the Saccharomyces lux SRCM100953 strain.
본 발명의 목적을 달성하기 위하여, 본 발명은 In order to achieve the object of the present invention, the present invention
(a) 쌀을 수침한 후 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 발효하여 쌀 코지를 제조하는 단계;(a) immersing the rice, increasing it, cooling it, inoculating the Aspergillus duckweed strain, and fermenting it to prepare rice koji;
(b) 상기 (a)단계의 제조한 쌀 코지에 물, 미강 및 소금을 혼합한 혼합물에 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 접종한 후 발효하여 미강 발효액을 제조하는 단계;(b) Zygosaccharomyces lux ( Zygosaccharomyces) in a mixture of water, rice bran, and salt in the rice koji prepared in step (a) rouxii ) preparing a rice bran fermentation broth by fermenting after inoculating the strain culture broth;
(c) 증자쌀, 소금, 고춧가루 및 메줏가루와 상기 (b)단계의 제조한 미강 발효액을 혼합한 후 발효하는 단계; 및(c) fermenting after mixing the steamed rice, salt, red pepper powder, and soybean meal with the fermented rice bran fermentation broth prepared in step (b); And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루, 물엿 및 조청을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법을 제공한다.(d) it provides a method for producing a red pepper paste using a rice bran fermented liquid, characterized in that it comprises the step of mixing red pepper powder, starch syrup, and jocheong with the fermented gochujang fermented product of step (c).
본 발명의 고추장의 제조방법에서, 상기 (a)단계의 쌀 코지는 바람직하게는 쌀을 4~6시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 28~32℃에서 44~52시간 동안 발효하여 제조할 수 있으며, 더욱 바람직하게는 쌀을 5시간 동안 수침한 후 121℃에서 10분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 30℃에서 48시간 동안 발효하여 제조할 수 있다. 상기와 같은 조건으로 쌀을 수침한 후 물빼기 및 증자하는 것이 적절히 증자됨으로써 미생물이 이용하는데 적합한 호화도와 과다한 증자에 의한 혐기적 조건 유발로 인한 잡균 오염 등을 방지할 수 있었고, 또한, 상기와 같은 발효과정을 통해 고추장 제조 시 발효를 촉진시킬 수 있는 쌀 코지로 제조할 수 있었다.In the method for producing red pepper paste of the present invention, the rice koji of step (a) is preferably immersed in rice for 4 to 6 hours, then steamed at 110 to 130°C for 8 to 12 minutes and cooled to aspergillus duck material. It can be prepared by inoculating the strain and fermenting it at 28 to 32°C for 44 to 52 hours.More preferably, the rice is soaked for 5 hours and then increased at 121°C for 10 minutes and cooled to obtain the Aspergillus duckweed strain. It can be prepared by inoculation and fermentation at 30° C. for 48 hours. After immersing rice under the above conditions, draining and increasing the water were appropriately increased, so that the gelatinization suitable for use by microorganisms and contamination of various bacteria caused by anaerobic conditions caused by excessive increase could be prevented. Through the fermentation process, it could be made into rice koji that can promote fermentation when making gochujang.
또한, 본 발명의 고추장의 제조방법에서, 상기 (b)단계의 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주는 지고사카로마이세스 룩시 SRCM100945 균주로, 상기 특정 균주를 이용하여 제조한 미강 발효액은 아미노태 질소 함량이 높으면서 풍미가 우수한 미강 발효액을 제조할 수 있었다.In addition, in the method for producing red pepper paste of the present invention, the Zygosaccharomyces lux of step (b) rouxii ) strain is the Saccharomyces lux SRCM100945 strain, and the rice bran fermentation broth prepared using the specific strain was able to prepare a rice bran fermentation broth with high amino nitrogen content and excellent flavor.
또한, 본 발명의 고추장의 제조방법에서, 상기 (b)단계의 미강 발효액은 바람직하게는 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 제조할 수 있으며, 더욱 바람직하게는 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 제조할 수 있다. 상기와 같은 조건으로 제조된 미강 발효액을 첨가한 고추장은 식이섬유 함량이 높고, 감칠맛 및 풍미가 증진되는 이점이 있다. 또한, 상기와 같은 조건으로 발효하는 것이 미강 발효액을 보다 경제적으로 발효하면서 아미노태 질소 함량이 높고 풍미도 증진시킬 수 있었다.In addition, in the manufacturing method of the red pepper paste of the present invention, the fermented rice bran in step (b) is preferably mixed with 180-240 g of rice koji, 450-550 mL of water, 180-240 g of rice bran, and 60-80 g of salt. The mixture can be prepared by inoculating 0.8-1.2% (v/v) strain culture solution of Zygosaccharomyces rouxii relative to the mixture and then fermenting it at 28-32° C. for 6-8 days, and more preferably To a mixture of 210 g of rice koji, 500 mL of water, 210 g of rice bran and 70 g of salt, compared to the mixture, Zygosaccharomyces rouxii ) can be prepared by fermenting for 7 days at 30° C. after inoculating 1% (v/v) strain culture. Gochujang added with fermented rice bran fermented under the above conditions has a high dietary fiber content, and has the advantage of enhancing umami and flavor. In addition, fermentation under the above-described conditions allowed the rice bran fermentation broth to be fermented more economically, while the amino nitrogen content was high and flavor was improved.
또한, 본 발명의 고추장의 제조방법에서, 상기 (c)단계는 바람직하게는 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g, 메줏가루 70~90 g 및 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효할 수 있으며, 더욱 바람직하게는 증자쌀 80 g, 소금 40 g, 고춧가루 90 g, 메줏가루 80 g 및 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효할 수 있다. 상기와 같은 조건으로 발효하는 것이 이미 및 이취가 발생하지 않으면서 아미노태 질소 함량이 높아 품질이 우수한 고추장 제조에 적합한 조건으로 발효할 수 있었다.In addition, in the manufacturing method of the red pepper paste of the present invention, the step (c) is preferably steamed rice 70-90 g, salt 35-45 g, red pepper powder 80-100 g, meso powder 70-90 g, and rice bran fermentation liquid 650- After mixing 750 g, it can be fermented at 28 to 32°C for 18 to 24 days, more preferably steamed rice 80 g, salt 40 g, red pepper powder 90 g, meso powder 80 g, and rice bran fermentation liquid 700 g After that, it can be fermented for 21 days at 30°C. Fermentation under the same conditions as described above could be fermented under conditions suitable for production of high-quality red pepper paste due to the high amino nitrogen content without already occurring and off-flavor.
본 발명의 미강 발효액을 이용한 고추장의 제조방법에서, 상기 (d)단계의 혼합은 바람직하게는 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합할 수 있으며, 더욱 바람직하게는 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합할 수 있다. 상기 (c) 및 (d)단계의 재료 및 배합비로 제조된 고추장은 재료들의 맛과 향이 잘 어우러져 고추장의 매운맛, 짠맛, 구수한 맛 및 감칠맛이 잘 조화되어 풍미가 우수하여 기호도를 향상시킬 수 있었다. 또한, 고춧가루 재료를 상기 (c) 및 (d)단계의 2단계에 걸쳐 배합함으로써 갈변에 의한 고추장 색상의 선호도 저하를 막을 수 있었다.In the method for producing red pepper paste using the rice bran fermentation broth of the present invention, the mixing in step (d) is preferably 80 to 100 g of red pepper powder, 180 to 220 g of starch syrup, and 45 to 55 g of jocheong in the fermented red pepper paste, and , More preferably, 90 g of red pepper powder, 200 g of starch syrup, and 50 g of jocheong may be mixed with the fermented pepper paste. The red pepper paste prepared with the ingredients and mixing ratio of the above steps (c) and (d) was well harmonized with the taste and aroma of the ingredients, and the spicy, salty, savory and umami taste of the red pepper paste were well harmonized, so that the flavor was excellent, and the preference could be improved. In addition, by mixing the red pepper powder material over the two steps of steps (c) and (d), it was possible to prevent a decrease in the preference of the color of red pepper paste due to browning.
본 발명의 미강 발효액을 이용한 고추장의 제조방법은, 보다 구체적으로는The manufacturing method of red pepper paste using the rice bran fermentation broth of the present invention, more specifically
(a) 쌀을 4~6시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 28~32℃에서 44~52시간 동안 발효하여 쌀 코지를 제조하는 단계;(a) After soaking rice for 4-6 hours, increase the rice at 110-130℃ for 8-12 minutes, cool it, inoculate Aspergillus duckweed strain, and ferment it at 28-32℃ for 44-52 hours. Manufacturing a;
(b) 상기 (a)단계의 제조한 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 미강 발효액을 제조하는 단계;(b) To a mixture of 180-240 g of rice koji prepared in step (a) with 450-550 mL of water, 180-240 g of rice bran, and 60-80 g of salt, compared to the mixture, Zygosaccharomyces rouxii ) preparing a rice bran fermentation broth by fermenting for 6-8 days at 28-32°C after inoculating 0.8-1.2 % (v/v) strain culture broth;
(c) 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g 및 메줏가루 70~90 g과 상기 (b)단계의 제조한 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효하는 단계; 및(c) After mixing 70 to 90 g of steamed rice, 35 to 45 g of salt, 80 to 100 g of red pepper powder and 70 to 90 g of buckwheat flour and 650 to 750 g of the fermented rice bran fermented liquid prepared in step (b), 28 to 32 Fermenting for 18 to 24 days at °C; And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합하는 단계를 포함할 수 있으며,(d) mixing 80 to 100 g of red pepper powder, 180 to 220 g of starch syrup, and 45 to 55 g of jocheong in the fermented gochujang fermented product of step (c),
더욱 구체적으로는More specifically
(a) 쌀을 5시간 동안 수침한 후 121℃에서 10분 동안 증자하고 냉각하여 아스퍼질러스 오리재 균주를 접종하고 30℃에서 48시간 동안 발효하여 쌀 코지를 제조하는 단계;(a) preparing rice koji by inoculating the Aspergillus duckweed strain by soaking rice for 5 hours and then increasing the rice for 10 minutes at 121°C and cooling, and fermenting the rice at 30°C for 48 hours;
(b) 상기 (a)단계의 제조한 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 미강 발효액을 제조하는 단계;(b) To a mixture of 210 g of rice koji prepared in step (a) with 500 mL of water, 210 g of rice bran, and 70 g of salt, compared to the mixture, Zygosaccharomyces rouxii ) strain culture solution was 1% ( v/v) preparing a rice bran fermentation broth by fermenting at 30° C. for 7 days after inoculation;
(c) 증자쌀 80 g, 소금 40 g, 고춧가루 90 g 및 메줏가루 80 g과 상기 (b)단계의 제조한 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효하는 단계; 및(c) mixing 80 g of steamed rice, 40 g of salt, 90 g of red pepper powder, 80 g of buckwheat powder and 700 g of the fermented rice bran fermentation broth prepared in step (b) and fermenting at 30° C. for 21 days; And
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합하는 단계를 포함할 수 있다.(d) mixing 90 g of red pepper powder, 200 g of starch syrup, and 50 g of jocheong to the fermented gochujang fermented product of step (c).
본 발명은 또한, 상기 방법으로 제조된 미강 발효액을 이용한 고추장을 제공한다.The present invention also provides a gochujang using the rice bran fermented broth prepared by the above method.
이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, preparation examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Preparation Examples and Examples.
제조예Manufacturing example 1. One. 미강Rice bran 발효액을 이용한 고추장 Gochujang using fermented liquid
(a) 쌀에 물을 첨가하여 5시간 동안 침지한 후 121℃에서 10분 동안 증자하고 30℃로 냉각한 밥에 아스퍼질러스 오리재(Aspergillus oryzae) 균주(105~6 CFU/ml) 0.01%를 접종하고 30℃ 및 습도 80% 조건에서 48시간 동안 발효하여 쌀 코지를 제조하였다.(a) After adding water to rice, immersing it for 5 hours, steaming for 10 minutes at 121°C and cooling to 30°C, Aspergillus duck material ( Aspergillus oryzae ) strain (10 5-6 CFU/ml) 0.01% was inoculated and fermented at 30° C. and 80% humidity for 48 hours to prepare rice koji.
(b) 상기 (a)단계의 제조한 쌀 코지 210 g에 물 500 mL, 미강 210 g 및 소금 70 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액(105~6 CFU/ml)을 1%(v/v) 접종한 후 30℃에서 7일 동안 발효하여 미강 발효액을 제조하였다.(b) To a mixture of 210 g of rice koji prepared in step (a) with 500 mL of water, 210 g of rice bran and 70 g of salt, compared to the mixture, Zygosaccharomyces rouxii ) strain culture solution (10 5 ~ 6 CFU/ml) was inoculated with 1% (v/v) and fermented at 30° C. for 7 days to prepare a rice bran fermentation broth.
(c) 쌀을 5시간 동안 수침한 후 110~130℃에서 8~12분 동안 증자한 쌀 또는 상기 증자한 쌀을 분쇄한 호화 쌀가루 80 g과 소금 40 g, 고춧가루 90 g 및 메줏가루 80 g과 상기 (b)단계의 제조한 미강 발효액 700 g을 혼합한 후 30℃에서 21일 동안 발효하였다.(c) After soaking the rice for 5 hours, rice steamed at 110 to 130°C for 8 to 12 minutes or 80 g of crushed luxury rice flour and 40 g of salt, 90 g of red pepper powder and 80 g of meso powder and After mixing 700 g of the rice bran fermentation broth prepared in step (b), it was fermented at 30° C. for 21 days.
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 90 g, 물엿 200 g 및 조청 50 g을 혼합한 후, 80℃에서 15분 동안 살균하였다.(d) 90 g of red pepper powder, 200 g of starch syrup, and 50 g of jocheong were mixed with the fermented gochujang fermented product of step (c), and then sterilized at 80° C. for 15 minutes.
1. 재료 및 방법1. Materials and methods
1) 재료1) material
고추장 제조에 사용한 쌀, 대두, 고춧가루 등의 재료는 국내산(전북 순창군)을 사용하였다. 고추장을 발효하는데 사용하는 균주는 크게 탄수화물 분해능과 단백질 분해능이 우수한 아스퍼질러스 오리재(Aspergliius oryzae) 균주와 바실러스 서브틸리스(Bacillus subtilis) 균주이며, 곰팡이 균주는 충무발효 종균을 구입하여 사용하여 쌀코지를 제조하였고, 고초균인 바실러스 균주는 당사에서 메주발효에 사용되는 보관 균주를 사용하여 메줏가루를 제조하였다. 또한 고추장에 풍미를 주기 위한 효모로써 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주를 (재)발효미생물산업진흥원으로부터 분양받아서 사용하였다.Ingredients such as rice, soybeans, and red pepper powder used to make gochujang were domestically produced (Sunchang-gun, Jeonbuk). The strain used for fermentation of red pepper paste is Aspergliius Duck ( Aspergliius) , which has excellent carbohydrate resolution and protein resolution. oryzae ) strain and Bacillus subtilis strain, and the fungal strain was made by purchasing and using the chungmu fermented seed, and the Bacillus strain, the Bacillus strain, was used for meju fermentation. The powder was prepared. Also, Zygosaccharomyces lux ( Zygosaccharomyces) is a yeast for giving flavor to red pepper paste. rouxii ) strain was purchased and used from the (Re) Fermentation Microorganism Industry Promotion Agency.
2) 실험방법2) Experiment method
(가) 수분측정(A) Moisture measurement
시료 5 g씩 채취하여 적외선 수분측정기에서 수분을 측정하였으며, 3 반복 진행하였다.5 g of samples were collected each, and the moisture was measured with an infrared moisture meter, and the process was repeated 3 times.
(나) pH 및 적정 산도 측정 (B) Measurement of pH and proper acidity
시료를 채취하여 믹서기로 마쇄한 후 무게 5 g을 취하고 증류수를 가하여 50 mL로 맞춘 후 이를 충분히 혼합한 후 pH 미터(Mettler Toledo Gmbh, Switzerland) 를 이용하여 측정하였고, 적정 산도는 0.1N-NaOH를 가하여 pH 8.4가 될 때까지 적정하였다.After taking a sample and grinding it with a blender, weigh 5 g, add distilled water to adjust it to 50 mL, mix it sufficiently, and measure it using a pH meter (Mettler Toledo Gmbh, Switzerland), and the appropriate acidity was 0.1N-NaOH. And titrated until the pH reached 8.4.
(다) 아미노태 질소(amino-type nitrogen) 측정(C) Measurement of amino-type nitrogen
아미노태 질소는 Formol법으로 측정하였다. 시료 1 g을 비커에 취하고 증류수 50 mL를 가하고 240초 동안 진탕 혼합하여 충분히 용해한 다음 0.1N-NaOH 용액으로 적정하여 pH 8.4가 되도록 적정하였다. 여기에 중성 포르말린(formalin) 20 mL를 가하고 20초 동안 진탕 혼합한 뒤 다시 0.1N-NaOH 용액으로 pH 8.4가 되도록 중화 적정하였다.Amino nitrogen was measured by the Formol method. 1 g of a sample was taken in a beaker, 50 mL of distilled water was added, shaken for 240 seconds, sufficiently dissolved, and then titrated with a 0.1N-NaOH solution to obtain a pH of 8.4. To this, 20 mL of neutral formalin was added and mixed with shaking for 20 seconds, followed by neutralization titration with 0.1N-NaOH solution to pH 8.4.
(라) 관능검사(D) Sensory test
본 발명의 고추장의 출시가능성을 최종 점검하기 위해 관능특성을 분석하였다. 보다 상세하게는 1단계의 풍미 기호도 분석은 연구원을 대상으로 간이 실험을 실시하고 대량 pilot-scale로 가기 위한 기초자료로써 활용하였다. 2단계 풍미 기호도 분석(pilot-scale)은 평점법에서 항목척도를 사용하여 5점 척도로 평가토록 하며 농업회사법인 순창장류(주)의 전직원을 대상으로 짠맛과 감칠맛 농도별 미맹테스트를 진행한 후 선발된 인원인 12명을 대상으로 실시하였다.Sensory characteristics were analyzed in order to finally check the marketability of the red pepper paste of the present invention. In more detail, the flavor preference analysis in step 1 was used as basic data for conducting a simple experiment for researchers and going to a mass pilot-scale. The second-stage flavor preference analysis (pilot-scale) is evaluated on a 5-point scale using an item scale in the rating method, and after conducting a tasteless test by concentration of salty and umami taste for all employees of Sunchang Paste Co., Ltd. It was conducted with 12 selected personnel.
(마) 식이섬유 측정(E) Dietary fiber measurement
고추장 제조 후 1차적으로 전북대학교 바이오식품소재개발 및 산업화연구센터에 의뢰하여 식이섬유 함량을 분석하도록 하고, 최종적으로 과제 제출용으로 공인분석기관에 의뢰하여 식이섬유량에 대한 분석결과를 확보하였다.After the production of red pepper paste, the first request was made to the Chonbuk National University Biofood Material Development and Industrialization Research Center to analyze the content of dietary fiber, and finally, the result of the analysis on the amount of dietary fiber was obtained by requesting an authorized analysis agency for the submission of the project.
실시예Example 1. One. 미강Rice bran 발효액 제조를 위한 균주 선발 Selection of strains for manufacturing fermentation broth
실험 균주는 하기 표 1과 같이 (재)발효미생물산업진흥원으로부터 균주를 제공받아 사용하였으며, 미강 혼합물 제조 후 YM 배지에서 액상 배양한 효모 배양액은 0.3%씩 접종하여 30℃에서 7일 동안 발효를 진행하였다. 풍미 관능검사와 아미노태 질소(AN) 분석치를 기준으로 1차로 3종의 효모를 분리하였다. 균주 선발을 위한 관능검사의 경우 간이실험으로 진행하였으며 연구원을 대상으로 직접 향을 맡아보고 1부터 7까지(1점: 매우 좋지않음, 7점: 매우 좋음)의 점수를 주도록 하였으며 실험결과는 표 2와 같이 나타내었다.The experimental strain was used by receiving a strain from the (Re) Fermentation Microorganisms Industry Promotion Agency as shown in Table 1 below.After the rice bran mixture was prepared, the yeast culture broth cultured in a liquid phase in YM medium was inoculated by 0.3% and fermented at 30°C for 7 days. I did. Three types of yeast were firstly isolated based on the flavor sensory test and amino-type nitrogen (AN) analysis. In the case of the sensory test for strain selection, a simple experiment was conducted, and the researcher directly smelled the fragrance and gave a score of 1 to 7 (1 point: very poor, 7 points: very good). It is expressed as
그 결과, 상대적으로 1번, 3번, 5번, 9번, 10번 균주의 풍미 선호도가 우수한 것으로 나타났으며, 아미노태 질소 함량은 1번, 5번 및 9번 균주에서 높게 나타났다. 따라서 관능 선호도가 높으면서 아미노태 질소 수치가 상대적으로 높게 나타난 1번, 5번, 9번 균주인 지고사카로마이세스 룩시 SRCM100945, 지고사카로마이세스 룩시 SRCM100949, 지고사카로마이세스 룩시 SRCM100953의 3가지 균주를 고추장 제조 비교 실험에 사용하도록 하였다.As a result, it was found that the flavor preference of strains 1, 3, 5, 9, and 10 was relatively excellent, and the amino nitrogen content was high in strains 1, 5 and 9. Therefore, three types of strains 1, 5, and 9, which have high sensory preference and relatively high amino-nitrogen levels, are Saccharomyces lux SRCM100945, Saccharomyces lux SRCM100949, and Saccharomyces lux SRCM100953. The strain was to be used in a comparative experiment for manufacturing gochujang.
실시예Example 2. 발효조건에 따른 2. According to fermentation conditions 미강Rice bran 발효액 최적 발효조건 설계 Design of optimal fermentation conditions for fermentation broth
최적 발효 조건의 설계를 위해 선별된 3종의 발효 균주를 사용한 미강 발효액을 30℃에서 7일과 14일간 발효를 진행하여 품질 특성을 분석해 보았다. 미강 발효액은 발효기간을 달리하여 제조한 후 pH, 아미노태 질소 및 관능 풍미를 분석하였으며, 그 결과는 표 3에 나타내었다.For the design of optimal fermentation conditions, quality characteristics were analyzed by fermenting rice bran fermentation broth using three fermentation strains selected at 30°C for 7 days and 14 days. The rice bran fermentation broth was prepared by varying the fermentation period, and then the pH, amino nitrogen and sensory flavor were analyzed, and the results are shown in Table 3.
pH는 7일 발효 후 5.6~5.8 수준이었으며 14일 발효시 5.4~6.0을 나타내었다. 9번 균주를 이용한 미강 발효액의 경우 14일 발효시 항온항습기 보관 중 표면 효모가 관찰되었으며 좋지 않은 풍미가 나타났다. 1번과 5번 균주를 이용한 미강 발효액의 경우 7일 발효와 14일 발효의 풍미 차이가 크지는 않았으나, 발효가 진행됨에 따라 물성이 매우 묽은 것으로 나타나 차후 소재로 사용시 물성 품질의 조정에 애로 사항이 발생할 것으로 판단되었다. 아미노태 질소(AN) 함량은 발효기간이 늘어남에 따라 일부 증가하였으나 원료로부터 오는 함량에 한계가 있어 차이가 크지 않은 것으로 판단된다. 이상과 같이 발효기간의 연장이 대량 생산시 상기와 같은 문제점이 발생하는 바, 효모를 이용한 미강 발효액의 최적 발효조건으로는 30℃에서 7일간 발효하는 것이 품질 표준화 제품 생산에 유리할 것으로 판단된다.The pH ranged from 5.6 to 5.8 after 7 days fermentation and 5.4 to 6.0 at 14 days fermentation. In the case of rice bran fermentation broth using strain No. 9, surface yeast was observed during storage in a thermo-hygrostat during fermentation for 14 days, and a bad flavor was observed. In the case of rice bran fermentation broth using strains 1 and 5, the difference in flavor between the 7-day fermentation and the 14-day fermentation was not significant, but as the fermentation proceeded, the physical properties were found to be very thin, making it difficult to adjust the quality of properties when used as a later material. It was judged to occur. The content of amino nitrogen (AN) partially increased as the fermentation period increased, but there is a limit to the content from the raw materials, so the difference is not considered to be significant. As described above, the prolongation of the fermentation period causes the above problems when mass-produced. As an optimal fermentation condition for the rice bran fermentation broth using yeast, fermentation at 30° C. for 7 days is considered to be advantageous for the production of quality standardized products.
(7점 척도법)Sensory test
(7-point scale)
실시예Example 3. 3. 미강Rice bran 발효액을 최적화할 수 있는 우량 균주의 선발(2차) Selection of superior strains that can optimize fermentation broth (secondary)
1차 선발과정을 거친 3종의 균주를 이용한 미강 발효액으로 고추장을 제조한 후 품질을 비교 분석함으로써 최종 1종의 균주를 선발코자 하였다. 고추장 발효는 3주간 실시하였으며, 이는 발효를 오래 하더라도 아미노태 질소 수치의 급격한 상승은 없었으며, 오히려 과 발효시 pH의 하락으로 이미나 이취가 생기는 문제가 있어 3주간 발효하였다.Gochujang was prepared with fermented rice bran fermented broth using three strains that went through the first selection process, and the quality was compared and analyzed to select the final strain. Gochujang fermentation was carried out for 3 weeks, and even though fermentation was prolonged, there was no sharp increase in amino nitrogen levels. Rather, there was a problem that an off-flavor was formed due to a drop in pH during over-fermentation, so the fermentation was performed for 3 weeks.
균주 종류 및 발효기간에 따른 고추장의 수분, pH, AN, 적정 산도를 분석한 결과는 도 1 내지 4와 같다. 수분 함량은 고추장을 숙성하는 과정에서 약 3% 가량 증가하는 것으로 나타났으며(도 1), pH는 초기에 비해 하락하여 5.5~5.6 수준을 나타내었다(도 2). 이때 pH의 하락 패턴은 균주별 차이를 보였는데, 1번 균주를 이용한 1번 고추장 발효물의 경우 단계별로 떨어졌고, 5번, 9번 균주를 이용한 5번 고추장 발효물 및 9번 고추장 발효물의 경우 발효 초기에 급격히 떨어지다가 일정수준으로 유지하는 것으로 나타났다. 아미노태 질소 함량은 발효기간이 증가함에 따라 상승하여 3주 발효 후 200 mg% 이상을 나타내었고, 1번 균주를 이용한 고추장 발효물이 가장 높게 나타났다(도 3). 발효기간에 따른 고추장 발효물 적정 산도의 변화는 발효 초기에 증가하였다가 시간이 갈수록 감소하는 경향이 나타났으며, 1번 균주를 이용한 고추장 발효물이 산도가 상대적으로 높아 풍미 유기산 등의 함량이 높은 것으로 판단할 수 있었다(도 4). 본 실험결과를 토대로 1번 균주를 이용하는 것이 고품질의 고추장 제조에 가장 적합할 것으로 판단되어, 1번 균주인 지고사카로마이세스 룩시 SRCM100945 균주를 고추장에 첨가되는 미강 발효액 제조용 효모균으로 최종 선발하였다.The results of analyzing moisture, pH, AN, and proper acidity of Gochujang according to the strain type and fermentation period are shown in FIGS. 1 to 4. The moisture content was found to increase by about 3% during the aging process of red pepper paste (FIG. 1), and the pH decreased compared to the initial stage, indicating a level of 5.5 to 5.6 (FIG. 2). At this time, the pH drop pattern showed a difference by strain. In the case of fermented Gochujang No.1 using strain No.1, it fell step by step, and fermented Gochujang No.5 fermented product using strain No.5 and No.9 and fermented Gochujang No.9 fermented product. It was found that it fell sharply at the beginning and maintained at a certain level. The amino nitrogen content increased as the fermentation period increased, indicating 200 mg% or more after 3 weeks of fermentation, and the fermented product of red pepper paste using strain 1 was the highest (FIG. 3). Changes in the proper acidity of fermented gochujang products according to the fermentation period increased at the beginning of fermentation and then decreased with time, and the acidity of the fermented gochujang fermented product using strain 1 was relatively high, and the content of flavored organic acids was high. It could be determined that it was (Fig. 4). Based on the results of this experiment, it was judged that the use of strain 1 would be most suitable for manufacturing high-quality red pepper paste, and strain 1, Saccharomyces luxy SRCM100945 strain, was finally selected as a yeast for making fermented rice bran added to red pepper paste.
실시예Example 4. 4. 미강Rice bran 발효액을 이용한 고추장 관능검사 Sensory test using fermented broth
실시예 3에서 선정된 지고사카로마이세스 룩시 SRCM100945 균주로 제조된 미강 발효액을 이용한 고추장과 상기 미강 발효액을 사용하지 않고 제조한 고추장(대조구)을 가지고 관능검사를 실시한 결과는 하기 표 4와 같다.The results of a sensory test with gochujang prepared without using the rice bran fermentation broth and gochujang (control) prepared without using the rice bran fermentation broth prepared with the Chigo Saccharomyces lux SRCM100945 strain selected in Example 3 are shown in Table 4 below.
그 결과, 대조구 고추장에 비해 본 발명의 제조예 1의 고추장은 감칠맛 및 풍미가 우수하여 고부가가치 셀링 포인트(selling point)를 갖는 제품으로 판단된다.As a result, the red pepper paste of Preparation Example 1 of the present invention is superior to that of the control red pepper paste, and is judged to be a product having a high value-added selling point.
실시예Example 5. 고추장 시제품의 가스 발생 유무 5. Whether or not gas is generated in the prototype of Gochujang
제조예 1의 고추장 시제품의 유통 안정성 여부를 판단하기 위해, 고추장 제품의 가스 생성 여부를 확인하였다. 미강 발효액 제조에 사용한 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주는 발효 시 가스를 발생시키는 균주로 알려져 있다. 제조예 1의 고추장과 제조예 1의 방법으로 제조하되 미강 발효액 무첨가 고추장(비접종)의 가스 발생 여부를 확인하였다. 제조된 고추장을 각각 50 g씩 PE(Polyethlene) 필름에 담아 밀봉한 후 30℃ 인큐베이터에서 일정기간 동안 팽창 부피를 체크하였다. 부피체크는 메스실린더에 물을 채운 후 샘플봉지에 넣어 늘어난 부피로 가스 발생 여부를 체크하였다.In order to determine whether or not the distribution stability of the Gochujang prototype product of Preparation Example 1, it was confirmed whether or not the gochujang product gas was generated. Zygosaccharomyces used to prepare rice bran fermentation broth rouxii ) strains are known to generate gas during fermentation. It was confirmed whether or not gas was produced in the red pepper paste of Preparation Example 1 and the red pepper paste (non-inoculated) prepared by the method of Preparation Example 1. Each 50 g of the prepared red pepper paste was put in a PE (Polyethlene) film and sealed, and the expansion volume was checked for a certain period in a 30°C incubator. The volume check was performed by filling the measuring cylinder with water and putting it in a sample bag to check whether gas was generated by the increased volume.
그 결과, 미강 발효액 무첨가 고추장과 미강 발효액 첨가 고추장 모두에서 가스발생은 관찰되지 않았다.As a result, gas generation was not observed in both gochujang without rice bran fermentation broth and gochujang with rice bran fermentation broth.
N.D: 검출되지 않음N.D: not detected
실시예Example 6. 고추장 시제품의 식이섬유 함량 분석 6. Analysis of Dietary Fiber Content of Gochujang Prototype
제조예 1의 고추장과 제조예 1의 방법으로 제조하되 미강 발효액 무첨가 고추장(대조구)의 식이섬유 함량을 분석한 결과는 하기 표 6과 같다. 그 결과, 미강 발효액을 사용한 고추장의 식이섬유 함량이 약 2.5 g 높은 7.72 g으로 높게 나타났다.The results of analyzing the dietary fiber content of the red pepper paste prepared by the method of Preparation Example 1 and the red pepper paste without the addition of fermented rice bran (control) are shown in Table 6 below. As a result, it was found that the dietary fiber content of gochujang using fermented rice bran broth was high, about 2.5 g to 7.72 g.
실시예Example 7. 고추장 재료 배합비에 따른 관능검사 7. Sensory test according to the mixing ratio of red pepper paste ingredients
상기 제조예 1의 방법으로 제조된 고추장, 상기 제조예 1의 방법으로 고추장을 제조하되 미강 발효액 및 고추장 재료 배합비를 달리하여 제조된 고추장(비교예 1 내지 4)을 가지고 5점 척도법으로 관능검사를 실시하였다.Gochujang prepared by the method of Preparation Example 1, and the gochujang prepared by the method of Preparation Example 1, but were prepared by varying the mixing ratio of fermented rice bran fermentation broth and gochujang ingredients (Comparative Examples 1 to 4), and a sensory test using a 5-point scale method. Implemented.
그 결과, 다른 재료 및 배합비로 제조된 비교예들의 고추장에 비해 제조예 1의 고추장이 외관, 감칠맛 및 향에 대한 기호도에서 더욱 선호하는 것을 확인할 수 있었고, 전반적인 기호도에서도 4.4점의 높은 점수를 나타냄을 확인할 수 있었다.As a result, it was confirmed that the red pepper paste of Preparation Example 1 was more preferred in the preference for appearance, umami, and aroma compared to the red pepper paste of Comparative Examples prepared with other ingredients and mixing ratios, and the overall preference also showed a high score of 4.4 points. I could confirm.
Claims (4)
(b) 상기 (a)단계의 제조한 쌀 코지 180~240 g에 물 450~550 mL, 미강 180~240 g 및 소금 60~80 g을 혼합한 혼합물에 혼합물 대비 지고사카로마이세스 룩시(Zygosaccharomyces rouxii) 균주 배양액을 0.8~1.2%(v/v) 접종한 후 28~32℃에서 6~8일 동안 발효하여 아미노태 질소 함량이 증진된 미강 발효액을 제조하는 단계;
(c) 증자쌀 70~90 g, 소금 35~45 g, 고춧가루 80~100 g 및 메줏가루 70~90 g과 상기 (b)단계의 제조한 미강 발효액 650~750 g을 혼합한 후 28~32℃에서 18~24일 동안 발효하는 단계; 및
(d) 상기 (c)단계의 발효한 고추장 발효물에 고춧가루 80~100 g, 물엿 180~220 g 및 조청 45~55 g을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 미강 발효액을 이용한 고추장의 제조방법.(a) After soaking rice for 4-6 hours, increase the rice at 110-130℃ for 8-12 minutes, cool it, inoculate Aspergillus duckweed strain, and ferment it at 28-32℃ for 44-52 hours. Manufacturing a;
(b) To a mixture of 180-240 g of rice koji prepared in step (a) with 450-550 mL of water, 180-240 g of rice bran, and 60-80 g of salt, compared to the mixture, Zygosaccharomyces rouxii ) preparing a rice bran fermentation broth with enhanced amino nitrogen content by fermenting for 6-8 days at 28-32°C after inoculating 0.8-1.2 % (v/v) strain culture broth;
(c) After mixing 70 to 90 g of steamed rice, 35 to 45 g of salt, 80 to 100 g of red pepper powder and 70 to 90 g of meso powder and 650 to 750 g of the fermented rice bran fermentation broth prepared in step (b), 28 to 32 Fermenting for 18 to 24 days at °C; And
(d) Gochujang using rice bran fermentation liquid, characterized in that it comprises mixing 80 to 100 g of red pepper powder, 180 to 220 g of starch syrup, and 45 to 55 g of jocheong in the fermented gochujang fermented product of step (c) Method of manufacturing.
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JP2002330723A (en) * | 2001-02-28 | 2002-11-19 | Samjo Celltech Ltd | Noodle using soybean embryo and method for producing the same and hishios produced by using the same |
KR101489999B1 (en) | 2012-10-31 | 2015-02-04 | 씨제이제일제당 (주) | Novel Aspergillus oryzae CJ 1354 isolated from the Korean traditional meju and the method of rice hot pepper paste using this fungi and the rice hot pepper paste from manufactured thereof |
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JP2002330723A (en) * | 2001-02-28 | 2002-11-19 | Samjo Celltech Ltd | Noodle using soybean embryo and method for producing the same and hishios produced by using the same |
KR101489999B1 (en) | 2012-10-31 | 2015-02-04 | 씨제이제일제당 (주) | Novel Aspergillus oryzae CJ 1354 isolated from the Korean traditional meju and the method of rice hot pepper paste using this fungi and the rice hot pepper paste from manufactured thereof |
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