KR20190056176A - Cosmetic comoposition for anti-wrinkle comprising Lactobacillus plantarum culture - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin wrinkles comprising Lactobacillus plantarum culture. The culture is obtained by a method comprising: (a) a step of inoculating a Lactobacillus plantarum strain into a medium and culturing the same; (b) a step of centrifuging the resultant product of the step (a); and (c) a step of recovering the supernatant from the resultant product of the step (b). Therefore, the present invention has a skin wrinkle alleviation effect.

Description

[0001] The present invention relates to a cosmetic composition for improving skin wrinkles comprising a culture of Lactobacillus plantarum (Lactobacillus plantarum culture containing an anti-

The present invention relates to a cosmetic composition having a collagen synthesis effect containing a single lactic acid bacterium culture, and more particularly to a cosmetic composition for promoting collagen synthesis and improving skin wrinkles comprising a culture of Lactobacillus plantanium CJLP133 .

Skin protects the body from various oxidants, air pollution, heavy metals, ultraviolet radiation (UV), etc. It prevents bacteria, fungus and virus from invading the skin and acts as a protective barrier against moisture loss. The structure of the skin is divided into three layers of epidermis, dermis and subcutaneous fat, and 90% of the dermis consists of collagen, elastin, hyaluronic acid and glycoprotein. Major components Collagen is an important fibrous protein that accounts for about 25% of the total protein mass of the human body. It occupies most of the connective tissue and functions as intercellular matrix, structural support function, cell division and differentiation induction, skin tensile strength Provide important functions such as providing. Such collagen has been known to be affected by aging, ultraviolet rays, noxious oxygen, and the like, and its structure is damaged, thereby closely affecting the wrinkling of the skin. In recent years, skin barriers are destroyed by environmental factors such as fine dusts, and skin aging is continuously promoted through reduction of collagen synthesis and increase of decomposition.

Therefore, maximizing the effects of each component and minimizing side effects to prevent aging of the skin is a very important problem in cosmetics. It promotes collagen synthesis, develops low-cost, low-cost materials and uses them It is essential to develop cosmetic products that are easy to use.

Lactic acid bacteria are beneficial in the human body to ferment carbohydrates and fiber to produce lactic acid (lactic acid). About 400 species have been found so far, of which 18 have been developed as products. When lactic acid bacteria enter the intestines of the human body, they secrete specific substances through their own metabolic activities. Since these substances have a beneficial effect on the human body, many studies on lactic acid bacteria have been made. Antimicrobial, antioxidant and immunological activities have been reported to date, and in recent years, whitening and moisturizing effects of lactic acid bacteria have been studied, and the possibility of becoming a flourishing material is increasing. Among the lactic acid bacteria, Lactobacillus has been reported to have a great effect on the immunity, antibacterial effect, skin whitening and atopic dermatitis.

Among them, POLAB (Plant Originated Lactic Acid Bacteria) is a lactic acid bacterium isolated from a fermented food of plant raw materials such as pickles, kimchi, and is referred to as an animal lactic acid bacteria. The vegetable lactic acid bacterium is more than 10 times more than the conventional animal lactic acid bacteria, and has excellent adaptability to the external environment. In particular, vegetable lactic acid bacteria isolated from kimchi, which have high salt content, low pH, and high content of natural antimicrobial substances, are produced in a harsh environment, and thus produce various physiologically active substances. The representative physiologically active substances include antimicrobial peptides, immunostimulating substances, and GABA (Gamma-Amino Butyric Acid), which exhibits anti-inflammatory and immunosuppressive effects among various physiological functions. Lactic acid bacteria also produce bacteriocins that act as antimicrobial peptides as heat-stable peptides. Bacteriocin is a natural antimicrobial protein or protein substance produced by microorganisms of various species, and generally refers to a substance having sterilizing activity against a species that is similar to the microorganism producing the same, or phylogenetically similar. Therefore, the development of cosmetic materials using the lactic acid bacteria culture fluid containing the above substances or the lactic acid bacteria fermentation plant extracts is active, which is in line with recent trends favoring environmentally friendly and safe substances.

Today, consumers are expected not only to expect general care effects on cosmetics, but also to have various functions such as anti-aging, anti-wrinkle, anti-irritation, anti-inflammatory and whitening effects. In recent years, various kinds of plant extracts have been used for the purpose of these cosmetics, and a new concept product is expected to show improved performance characteristics distribution by combining various various characteristics.

The present application verifies the effectiveness of the present invention as a cosmetic product that can be used for prevention of aging of skin, improvement of skin elasticity, and improvement of skin wrinkle through safer cultivation of lactic acid bacteria derived from Kimchi, without adverse effects on skin and increase of collagen biosynthesis, Furthermore, the present invention was made to examine the possibility of domestic industrial development, and it was confirmed that Lactobacillus plantarum CJLP133 culture had skin wrinkle improving activity, thus completing the present application.

KR 10-1486999 B1 (2015.01.21)

One object of the present application is to provide a process for the preparation of Lactobacillus plantarum The present invention also provides a cosmetic composition for improving skin wrinkles, which comprises a lactobacillus plantarum culture in which microorganisms of plantarum are removed.

As one aspect for solving the above problems, the present application provides a cosmetic composition for improving skin wrinkles comprising a lactobacillus plantarum culture in which cells of a Lactobacillus plantarum have been removed.

Hereinafter, the cosmetic composition for improving skin wrinkles containing the lactobacillus plantarum culture in the present application will be described in detail.

The term " Lactobacillus < RTI ID = 0.0 > plantarum "is one of the microorganisms of the genus Lactobacillus, is a gram-negative bacterium and is a microorganism producing lactic acid. It is found in fermented food such as sour crout, pickle, kimchi.

The Lactobacillus plantarum strain may specifically be a strain of Lactobacillus plantarum CJLP133, which may be one deposited with the deposit number KCTC 11403BP.

Specifically, the Lactobacillus plantarum CJLP133 strain was deposited internationally on October 16, 2008 under the Budapest Treaty with the KCTC 11403BP as an international depositary to the KCTC, an international depository institution of the Korea Research Institute of Bioscience and Biotechnology.

The term " culture product " in the present application means a culture medium containing a by-product produced by culturing a strain in a culture medium and ingesting nutrients and metabolizing the culture medium. The culture medium containing the diluted solution, concentrate, , A preparation of the culture medium, a preparation of the culture medium or a mixture thereof, and a culture of all the formulations which can be formed using the culture medium.

Specifically, the culture may be a culture obtained by culturing a Lactobacillus plantarum strain, and may be obtained by removing the microorganism of the strain after culturing. That is, a culture medium in which the strain is removed from the strain after culturing the strain may be used as a culture.

More specifically, the culture comprises: (a) inoculating and culturing a strain of Lactobacillus plantarum in a medium; (b) centrifuging the result of step (a); And (c) recovering the supernatant from the product of step (b).

In the step (a), the culture medium may be any medium used in the art, provided that the culture can be cultured and the culture after cultivation is obtainable. Specifically, the medium may be a liquid medium, more specifically, have.

The step (a) may further include a step of culturing the Lactobacillus plantarum strain in a solid medium and culturing the strain in the presence of the step (a) so as to obtain a purely isolated strain, thereby obtaining a confirmed colonies .

The step (b) may be carried out at a temperature of 1 ° C to 7 ° C, specifically 2 ° C to 6 ° C, more specifically 3 ° C to 5 ° C, using a high speed centrifuge at 1,500 rpm to 3,500 rpm, specifically 2,000 rpm to 3,000 rpm , More specifically, centrifuging at a rotational force of 2,200 rpm to 2,800 rpm for 10 minutes to 30 minutes, specifically 15 minutes to 25 minutes, more specifically 17 minutes to 23 minutes.

The step (c) may further comprise filtering the supernatant. The step (c) may further include a step of pulverizing the filtered supernatant using a freeze dryer.

The term " wrinkle " in the present application means a skin formed by aging of the skin, which can be caused by a cause of a gene, a collagen in a skin dermis, and an external environment.

In the present application, the composition may be to improve the wrinkles of the skin by enhancing the level of collagen synthesis to prevent the reduction of collagen.

The collagen is an important fibrous protein that accounts for about 25% of the total protein mass of the human body. It occupies most of the connective tissue and functions as an intercellular matrix, structural support function, induction of cell division and differentiation, and skin tensile strength ≪ / RTI >

The term in this application. &Quot; Improvement " refers to the use of the composition of the present application to inhibit or inhibit the symptoms of wrinkles of the skin, or alleviate the symptoms of skin wrinkles already present.

In the present application, the cosmetic composition according to the present invention can be used as a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, It may be formulated as a suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray, It is not limited.

In the present application, the cosmetic composition may further comprise at least one cosmetically acceptable carrier mixed with a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary components such as oil, water, a surfactant, a moisturizer, a lower alcohol , A thickening agent, a chelating agent, a coloring agent, a preservative, a flavoring agent, and the like, but is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present application varies depending on the formulation of the cosmetic composition.

When the formulations of the present application are ointments, pastes, creams or gels, it is preferable to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used as a carrier component. But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present application is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, an isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, a glycerol, a sugar and the like are used as a carrier component But is not limited thereto. These may be used alone or in combination of two or more.

The present application provides a method for producing a lactobacillus plantarum culture safe without side effects on the skin using the lactobacillus plantarum strain, which is a skin benefit bacterium, and provides a method for producing a lactobacillus plantarum culture product having a skin wrinkle- To provide a cosmetic composition.

Fig. 1 shows the results of collagen synthesis performance of the lactobacillus plantarum CJLP133 culture.

The present application will be described in more detail by the following examples. However, these embodiments are for illustrative purposes only, and the scope of the present application is not limited by these embodiments, and will be apparent to those skilled in the art to which the present application belongs.

Example  One. Lactobacillus Flora Room CJLP133  Preparation of culture

The Lactobacillus plantarum CJLP133 strain deposited with accession number KCTC 11403BP was plated on MRS solid medium (BD Difco, Lactobacilli MRS Agar) and incubated at 37 ° C for about 18 hours. The colonies identified as pure isolates were platinum loops and inoculated into MRS liquid medium (BD Difco, Lactobacilli MRS Broth) and incubated at 37 ° C for approximately 18 hours. After cultivation, the concentration of the culture was 1.0 * 10 ⁹ CFU / mL. The culture broth was centrifuged at 4 ° C and 2,500 rpm for 20 min using a high-speed centrifuge (LABOGENE 1248R) to separate the cells from the culture. The supernatant of the culture was filtered, and the filtered supernatant was pulverized using a freeze dryer (HETO PL90000). The culture prepared by the above method was called " Lactobacillus plantarum CJLP133 culture ".

Example  2. Lactobacillus Flora Room CJLP133  Confirmation of collagen synthesis effect of culture

The amount of collagen expression in the dermal fibroblast was measured to confirm the collagen synthesis effect of the lactobacillus plantarum CJLP133 culture prepared in Example 1 above.

Human fibroblast (NHDF) at a concentration of 5 * 10 ⁴ cells / dish was added to a 24-well plate in DMEM (Gibco, Dulbecco's modified eagle medium) containing 10% fetal bovine serum, The medium was added and incubated at 37 ° C for 48 hours. In the serum-free DMEM medium, the positive control (TGF-β 10 ng / mL), the experimental group MRS medium and the Lactobacillus plantarum CJLP133 culture were treated at 0.25 mg / mL, 0.5 mg / mL and 1.0 mg / Lt; / RTI > After the culture, the collagen synthesis performance was measured at 450 nm using a spectrophotometer using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay KIT, Takara).

As a result of the experiment, it was confirmed that the amount of collagen expression was increased in a concentration-dependent manner in the group treated with Lactobacillus plantarum CJLP133 culture. In particular, it was confirmed that the concentration of 3 (1 mg / mL) increased about 1.6 times as compared with that of the conventional MRS medium, which is equivalent to that of the positive control TGF-β, and that Lactobacillus plantarum CJLP133 It was confirmed that it has an excellent collagen synthesis performance against the cells. The experimental results are shown in Table 1 and FIG.

[Table 1]

From the above results, it was found that the lactobacillus plantarum CJLP133 culture of the present application is effective in reducing the synthesis of matrix proteins constituting the skin or increasing the degradation by the degrading enzyme to produce wrinkles. It was confirmed that the effect of improving the skin wrinkles was improved.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application is to be interpreted as being within the scope of the present application, all changes or modifications derived from the meaning and scope of the following claims and equivalents thereof, rather than the detailed description.

[Access number]

Institution name: Korea Biotechnology Research Institute

Accession number: KCTC11403BP

Payment date: 20081009

Claims (3)

Lactobacillus planta ( Latobacillus The present invention relates to a cosmetic composition for improving skin wrinkles, which comprises a lactobacillus plantarum culture in which microorganisms of plantarum have been removed.
The method according to claim 1,
Wherein the Lactobacillus plantarum strain is a Lactobacillus plantarum CJLP133 strain deposited with accession number KCTC 11403BP.
The method according to claim 1,
The culture comprises (a) inoculating and culturing a strain of Lactobacillus plantarum in a medium; (b) centrifuging the result of step (a); And (c) recovering the supernatant from the product of step (b).

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