KR20190055511A - Cosmetic composition containing tangerine fermented broth with lactic acid bacteria - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising fermented tangerine broth fermented with lactic acid bacteria and, more specifically, to a cosmetic composition comprising the fermented broth produced by fermenting tangerines with lactic acid bacteria. The cosmetic composition exhibits skin-exfoliating effects, skin-barrier enhancing effects, skin-restoring effects, and skin-brightening effects.

Description

[0001] The present invention relates to a cosmetic composition containing tangerine fermented broth with lactic acid bacteria,

The present invention relates to a cosmetic composition containing citrus fruits. More specifically, the present invention relates to a cosmetic composition containing a fermentation liquid made of citrus fruits and having skin exfoliating effect, skin barrier strengthening effect, skin regeneration effect and skin whitening effect.

Skin tissue consists of epidermis, dermis and hypodermis. Basal cells forming the basal layer at the innermost part of the epidermis undergo proliferation and division to differentiate into the stratum corneum, and epidermal cells are crossed with keratin to strengthen the interior. At the same time, desmosomes desmosomes) are linked to transient keratins to maintain the overall layer structure. During this differentiation process, the cell membrane is replaced by a unique structure called the cornified envelope. This structure is a membrane structure in which keratin and many insoluble proteins are cross-linked by an enzyme called transglutaminases (TGs) to form a crosslink. The structure is composed of the uppermost part of the epidermis, Lt; / RTI > It also protects the surface of the skin from external harmful environment by preventing the water molecules from leaking into the gap between the cells by closing and closing between the two cells and preventing moisture leakage in the skin.

Recovery is also very important because the epidermis, which is the part of the skin that determines the nature of the skin, is directly and frequently damaged from the outside. However, if the turnover period is long and it does not disappear naturally, the aging skin is removed by an artificial method called peeling. In this case, a chemical product using an acid component such as AHA (alpha hydroxy acid) and BHA (beta hydroxy acid), an enzyme product using a protease, and a physical product removing keratin using friction are used , These cosmetics cause skin irritation and weaken the skin surface when used repeatedly, which may break the skin barrier.

Here, the turnover cycle refers to a cycle in which the outer skin, which is the outermost part constituting the skin, is continuously peeled off. In addition, the turnover cycle refers to a process in which other cells growing underneath replace the place, and as the skin age deteriorates, the pigmentation of skin, freckles, dots, black spots, and skin aging and damage occur . Accordingly, in recent years, there has been an increasing demand for functional cosmetics for effectively exfoliating the skin without irritation and for promoting wound healing and skin regeneration of the skin.

On the other hand, citrus fruits are widely distributed in Korea, India, Myanmar, Malay Peninsula, Indochina, China, and Japan. Citrus fruits, lemons, oranges and grapefruits are typical examples. Currently, the citrus cultivated in Jeju Island is an alkaline food which uses all the ingredients from the grain to the skin. It smoothes the metabolism and strengthens the skin and the mucous membranes, thus preventing cold. It is good for skin beauty and fatigue recovery by the action of vitamin C, and helps absorption of calcium. Vitamin P (Hespyridine) inhibits the increase in permeability to capillaries, which helps prevent arteriosclerosis and hypertension.

Citrus fruits are generally rich in essential oils and aromatics, and flesh is rich in organic acids. Although there are many differences depending on the varieties, they contain about 1% on average, among which citric acid is a kind of alpha hydroxyacid. However, studies on cosmetic compositions containing citrus fruits and fermented liquid and showing a lesser stimulation than conventional AHA (alpha hydroxyacid) have been very limited.

Korean Patent Laid-Open No. 10-2017-0007637 (Apr. 19, 2017) discloses a pharmaceutical composition for prevention or treatment of bladder cancer and a food composition for preventing or ameliorating bladder cancer, which comprises a citrus comb secondary fermentation broth as an active ingredient. Korean Patent Laid-Open Publication No. 10-1999-0079683 (Nov. 11, 1999) discloses a functional health food containing citrus peel powder or peel extract.

The present invention relates to a cosmetic composition containing a fermentation broth made of citrus fruits, and it is intended to provide a cosmetic composition containing natural organic acids and having excellent skin exfoliation effect, skin barrier strengthening effect, skin regeneration effect and skin whitening effect do.

The present invention provides a cosmetic composition comprising a fermentation broth obtained by fermenting citrus with lactic acid bacteria.

In the cosmetic composition of the present invention, the lactic acid bacterium is preferably a strain belonging to the genus Lactobacillus sp.

In the lactic acid bacteria of the present invention, the strain of the genus Lactobacillus is more preferably a strain selected from the group consisting of Lactobacillus plantarum , Lactobacillus casei , and Lactobacillus paracasei It is good.

In the cosmetic composition of the present invention, it is preferable that the fermentation is performed by inoculating lactic acid bacteria at a concentration of 1 x 10 < ⁵ > to 10 < ⁷ > cfu / ml at 15 to 45 DEG C for 1 to 15 days.

In the cosmetic composition of the present invention, the citrus fruit may be an extract solution of citrus fruit pulp, or an extract obtained by adding water to citrus fruit powder.

In the cosmetic composition of the present invention, the cosmetic composition may be used for removing the exfoliating skin.

In the skin exfoliating cosmetic composition of the present invention, the cosmetic composition is preferably a soap or a cleanser.

In the cosmetic composition of the present invention, the cosmetic composition may preferably be for skin barrier strengthening.

In the cosmetic composition of the present invention, the cosmetic composition may preferably be for skin cell regeneration.

In the cosmetic composition of the present invention, the cosmetic composition may preferably be used for skin whitening.

The cosmetic composition containing the fermentation broth made of citrus fruits of the present invention contains natural organic acids and has excellent skin exfoliation effect, skin barrier strengthening effect, skin regeneration effect and skin whitening effect.

FIG. 1 is a photograph taken with a 'moritex viewer V2.' In order to evaluate the exfoliating effect of the citrus lactic acid fermentation broth of the present invention.
FIG. 2 is a graph showing the results of the expression amount of Transglutaminase 1 for measuring the skin barrier enhancing effect of the fermentation liquid of the present invention.
Fig. 3 is a photograph taken by a microscope to measure the skin cell regeneration effect of the citrus lactic acid fermentation broth of the present invention. Fig.
FIG. 4 is a graph showing inhibition rates of melanin production per unit cell to measure the skin whitening efficacy of the fermentation liquid of the present invention.

The present invention provides a cosmetic composition comprising a fermentation broth obtained by fermenting citrus with lactic acid bacterium (hereinafter, also referred to as "citrus lactic acid fermentation broth"). More specifically, in the present invention, lactic acid bacteria are inoculated into extracts of citrus fruit pulp or citrus pulp with purified water, and then fermented to prepare a fermentation liquid which can be added to the cosmetic composition.

The cosmetic composition of the present invention includes a fermentation broth obtained by fermenting citrus with lactic acid bacteria. In addition to the above components, other ingredients may be contained in the form of excipients and adjuvants for the purpose of providing stability or other functions of cosmetic formulations.

The formulation of the cosmetic composition of the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray , And it is preferably a soap or a cleanser.

The lactic acid bacterium to be used in the fermentation of the fermentation broth of the present invention is not particularly limited, but it is preferable to use a strain belonging to the genus Lactobacillus sp., More preferably lactobacillus plantarum It is preferable to use at least one strain selected from Lactobacillus plantarum , Lactobacillus casei and Lactobacillus paracasei .

When lactic acid bacteria are inoculated and fermented, specific fermentation conditions may be those of the art for lactic acid fermentation. Preferably, lactic acid bacteria are inoculated at a concentration of 1 × 10 ⁵ to 10 ⁷ cfu / ml and fermented at 15 to 45 ° C. for 1 to 15 days. In addition, a medium component (for example, skimmed milk powder or the like) for smooth culturing of lactic acid bacteria may be added to citrus fruits (citrus juice or citrus extract) if necessary.

On the other hand, according to the experiment of the present invention, the fermented product of citric acid lactic acid bacteria of the present invention has excellent skin exfoliation efficacy and excellent skin barrier enhancement efficacy. In addition, excellent skin cell regeneration efficacy was confirmed and excellent skin whitening efficacy was confirmed. Therefore, the fermented liquorice of the present invention can be used as an excellent cosmetic raw material.

Hereinafter, the present invention will be described in detail with reference to the following Production Examples and Experimental Examples. However, the scope of the present invention is not limited to the following production examples and experimental examples, and includes modifications of equivalent technical ideas.

[Preparation Example 1: Preparation of citrus fruit juice]

Citrus bark and pulp were selected as a sample and water was added thereto to prepare an extract. The extract was inoculated with Lactobacillus paracasei ATCC25599 at a concentration of 1 × 10 ⁶ cfu / ml to obtain 37 Lt; 0 > C for 7 days. After the incubation, the strain was inactivated and finally filtered using a filter. This was concentrated and used in the following experiments.

[Comparative Example 1-1: Preparation of fermentation broth of citron]

The bark and pulp of citron were selected as a sample, and an extract was obtained by adding water to extract or juice. The extract was inoculated with Lactobacillus paracasei ATCC25599 at a concentration of 1 × 10 ⁶ cfu / ml to obtain 37 Lt; 0 > C for 7 days. After the incubation, the strain was inactivated and finally filtered using a filter. This was concentrated and used in the following experiments.

[Comparative Example 1-2: Preparation of fermentation broth of lemon]

Lactobacillus paracasei ATCC25599 was inoculated at a concentration of 1 × 10 ⁶ cfu / ml to obtain an extract. The extracts were prepared by adding water to extracts and extracts of lemon peel and pulp. Lt; 0 > C for 7 days. After inoculation, the strain was inactivated and finally filtered using a filter. This was concentrated and used in the following experiments.

[Comparative Example 1-3: Preparation of fermentation broth of apple]

The extracts were prepared by inoculating the extracts with Lactobacillus paracasei ATCC25599 at a concentration of 1 × 10 ⁶ cfu / ml to prepare extracts. Lt; 0 > C for 7 days. After inoculation, the strain was inactivated and finally filtered using a filter. This was concentrated and used in the following experiments.

[ Comparative Example  2 : Non fermentation Citrus juice  Produce]

Citrus fruits were selected as a sample, and the extracts were prepared by juice filtration, followed by final filtration using a filter. This was concentrated and used in the following experiments.

[ Experimental Example  1: Skin irritation test]

For the above Production Example 1 and Comparative Example 2, 16 subjects were subjected to a skin patch test (Patch Test) using a Finn Chamber. only. Patients with psoriasis, eczema, other skin lesions, pregnant, lactating or contraceptive, and antihistamines were excluded from the study. Sixteen test subjects were wiped with 70% ethanol, dried and then dropped on a paper disc placed on the Finn chamber with the same amount of Sample and Control, and then fixed on the upper part of the test area.

After applying the patch for 24 hours and removing the patch, the test area was marked with a marking pen. After 30 minutes, 24 hours, and 48 hours, the test site was observed. The judgment was made after 30 minutes, 24 hours, and 48 hours after removal of the patch, and skin reactions were classified according to the criteria of the International Contact Dermatitis Research Group (ICDRG). The mean score was calculated according to the formula for calculating the average skin reactivity, and the presence or absence of stimulation was determined according to the skin patch test result. The results are shown in Table 1 below.

Name of sample ICDRG criteria Mean score Judgment Control group 1.39 Irritation Production Example 1
(Citrus fermentation solution) 1.39 Irritation Comparative Example 2
(Non fermented citrus liquid) 1.56 Light stimulus

As shown in the above Table 1, the mean score of Preparation Example 1 was 1.39, and the control group also had such a slight irritation. In Comparative Example 2, the irritation was 1.56. From the above results, it was found that Production Example 1 of the present invention had less skin irritation than Comparative Example 2.

[ Experimental Example  2: Evaluation of the exfoliation efficacy by keratinocyte diagnosis]

Twelve subjects (9 female, 3 male) aged 26 to 36 years were included in the study. Room temperature of 25 ° C, and room humidity of 55%. Twelve subjects were rinsed with flowing water through the wrist, wiped with Kleenex, and allowed to adapt to the external environment for 10 minutes. In addition, a test area of a certain size (4 cm ² , 2 cm) was designated on the inner part of the wrist, and a normal solid soap on the right side was cleaned with a soap containing 5% of the preparation example 1 on the left. For another person, the left side was washed with soap containing 5% of Comparative Example 2.

Before the experiment, wash the inside of the wrists of all subjects with running water, and immediately after wiping off the water with Kleenex, make a 10 minute adaptation to the external environment and tape stripping using D-squame . In addition, each test site was washed with soap containing a common solid soap and a sample, and the water was wiped with Kleenex. After 10 minutes, the keratin was collected through a tape stripping method.

The obtained keratin materials were observed through 'moritex viewer V2.' After attaching squam lens 50x to the charm view, and then photographing was used to evaluate the exfoliation efficacy. The experimental results are shown in Fig. 1 shows that when the inside of the wrist is washed with water, the stratum corneum is peeled off from the normal solid soap, the soap containing 5% of preparation example 1 and the soap containing 5% of comparison example 2, while the thick stratum corneum is present Respectively. Comparing the horny rinsed skin with the regular solid soap, the soap containing 5% of preparation example 1 and the soap containing 5% of comparison example, the control solid general soap and the 5% Production Example 1 was found to be effective for delamination in the case of containing 5% of Production Example 1, since the thickness of the stratum corneum was thinner and the exfoliation of the stratum corneum was more occurred when the soap containing 5% was used.

[ Experimental Example  3:  Skin barrier  Strengthening effect experiment]

In this Experimental Example, the effect of increasing the skin barrier-related gene expression was measured in the control group, preparation examples 1 to 2. Keratinocytes of human origin (HaCaT cell) to a density of 1.5 × 10 ⁶ cells in a DMEM added with 10% FBS 60 mm culture dish (culture dish) in inoculation and day in the 5% CO _2, at 37 ℃ incubator Lt; / RTI >

Thereafter, the medium was replaced with a fresh DMEM medium containing no FBS, and the control group, Preparation Examples 1 to 2, were treated for each concentration and cultured for 1 day. After 1 day, the cells were washed with 2 ml of phosphate buffered saline (PBS), treated with 1 ml of TriZol, transferred to a 1.5 ml tube and purified by phenol / chloroform extraction and ethanol precipitation. . After that, cDNA synthesis and real-time PCR were performed using quantified RNA and 'High Capacity RNA-to-cDNA kit (Applied Biosystems)'. ACTIN was used as a control, and primers of Transglutaminase 1 (TGase-1) used in real-time PCR were composed of SEQ ID NO: 1 and SEQ ID NO: 2, primers of actin SEQ ID NO: 3 and SEQ ID NO: 4. This is shown in the following Sequence Listing and Table 2. The results of the expression of Transglutaminase 1 are shown in Table 3 and FIG.

SEQ ID NO: 1 5'-TGG GTT ACA GAG GCC CAA GA-3 ' SEQ ID NO: 2 5'-GGC GCA GTG TCA CTG TTT CAT-3 ' SEQ ID NO: 3 5'-GGC ACC CAG CAC AAT GAA G-3 ' SEQ ID NO: 4 5'-CCG ATC CAC ACG GAG TAC TTG-3 '

Condition Experimental concentration (%) TGase1 expression level (%) Control group - 100.00 Production Example 1 0.5 155.66 Comparative Example 1-1 0.5 139.16 Comparative Example 1-2 0.5 121.29 Comparative Example 1-3 0.5 130.62 Comparative Example 2 0.5 141.43 Positive control group 1.2 mM calcium + A23187 0.1 [mu] g / ml 142.05

As shown in Table 3 and FIG. 2, Production Example 1 and Comparative Example 2 of the present invention showed an increase in TGase-1 expression level and a skin barrier enhancement effect, respectively, compared with the control group. In Example 1, TGase- Respectively. In addition, it was confirmed that TGase-1 expression was better than 1.2 mM calcium + A23187 used as a positive control.

[ Experimental Example  4: Cell regeneration effect test]

In this Experimental Example, the cell regeneration effect was measured in the control group, Production Example 1. Human fibroblast cells were inoculated in 96-well plates at a density of 1 × 10 ⁴ cells in IMDM supplemented with 10% FBS and incubated in a 5% CO ₂ incubator at 37 ° C for one day. After one day, the medium was removed, washed with 1 x PBS, and then replaced with a fresh IMDM medium containing no FBS, and treated with 0.25% of the control, Production Example 1 and cultured for 4 days. After 4 days, cells were photographed with a microscope. The results are shown in FIG. 3, it was confirmed that Production Example 1 of the present invention has cell regenerating effect more than the control group.

[ Experimental Example  5: Whitening effect test]

In this Experimental Example, the whitening boosting effect was measured with respect to Production Example 1. The effect of whitening boosting is measured by the method of Fukuda, J. Soc. Cosmetic Chem., 42 (361), 1991) by measuring the inhibitory effect of melanogenesis using B16-F1 melanoma cells Respectively.

The number of wells according to all concentrations was prepared in triplicate. Specifically, B16-F1 melanoma cells were inoculated into 6-well plates at a density of 1 × 10 ⁵ cells in DMEM supplemented with 10% FBS, and cultured in a 5% CO ₂ incubator at 37 ° C. for one day Lt; / RTI > After that, the medium was replaced with a fresh DMEM medium containing 10% FBS, and the sample was treated for each concentration and cultured for 3 days. After 3 days, the cells were washed with 2 ml of PBS (phosphate buffer, saline), treated with 0.25 ml of 1N NaOH, and the cells were collected in a 1.5 ml tube to obtain intracellular melanin. The collected cells were transferred to a 96-well plate, and the absorbance at 450 nm was measured. The inhibitory rate of melanin production per unit cell was calculated according to the following equations (1) and (2).

[Equation 1]

&Quot; (2) "

As a control group, arbutin (SK Bioland Co., Ltd.) at a concentration of 50, 100, and 250 / / ml was used. The experimental results are shown in Table 4 and FIG.

Percent inhibition of melanin production per unit cell (%) Arbutin ([mu] g / ml) 50 100 250 Arbutin 19.79 22.91 33.34 0.1% of Preparation Example 1 + arbutin 23.48 26.28 36.37 0.25% of Preparation Example 1 + arbutin 41.84 46.85 50.48 0.5% of Preparation Example 1 + arbutin 57.50 59.91 60.47

From Table 4 and FIG. 4, it was found that Production Example 1 of the present invention increased the inhibitory rate of melanin production per unit cell in a concentration-dependent manner when arbutin + Preparation Example 1 was treated together with arbutin alone. Thus, it can be concluded that Production Example 1 has a whitening effect.

[ Experimental Example  6: Comparison with fruit fermentation broth]

Preparation Example 1, Comparative Formulation Examples 1-1 to 1-3, and Comparative Formulation Example 2 were prepared using the above-described Preparative Example 1, Comparative Examples 1-1 to 1-3, and Comparative Example 2, .

ingredient
Content (% by weight) Formulation Example
One Comparative Formulation Example 1-1 Comparative Formulation Example 1-2 Comparative Formulation Example 1-3 Comparative Formulation Example 2 Control formulation example Production Example 1 10 - - - - - Comparative Example 1-1 - 10 - - - - Comparative Example 1-2 - - 10 - - - Comparative Example 1-3 - - - 10 - - Comparative Example 2
(Non fermented citrus liquid) - - - - 10 - cellulose 10 10 10 10 10 10 Macadamia nut oil 2 2 2 2 2 2 Squalane 3 3 3 3 3 3 Tocopheryl acetate 1.5 1.5 1.5 1.5 1.5 1.5 Purified water Balance Balance Balance Balance Balance Balance system 100 100 100 100 100 100

Sensory evaluation was carried out on a total of 15 persons, ranging in age from 20 to 40 in each age group. Each of the above-mentioned Formulation Example 1 and Comparative Formulation Examples 1-1 to 1-3 was applied to the right and left hands and massaged Skin irritation, degree of exfoliation, skin moisturization, overall acceptability. The highest score is 10 points, the average score is 5 points, and the score is 0 points. The scores in Table 6 are the average scores of the 15 responses.

Evaluation items Formulation Example 1 Comparative Formulation Example 1-1 Comparative Formulation Example 1-2 Comparative Formulation Example 1-3 Comparative Formulation Example 2 Control formulation example Degree of exfoliation 10 7 9 6 4.3 4 Degree of skin irritation 7.6 6.6 1.3 5 6.6 8.3 Skin moisturizing power 7.6 5.3 6.6 5.3 6.6 5.3 Total points (/ 30 points) 25.2 18.9 16.9 16.3 17.5 17.6 Average (10 points) 8.4 6.3 5.6 5.4 5.8 5.9

As shown in Table 6, Formulation Example 1 showed excellent results in comparison with Comparative Formulation Examples 1-1 to 1-3 and Comparative Formulation Example 2 and Comparative Formulation Example. In particular, Formulation Example 1 showed similar degree of exfoliation to Comparative Formulation Example 2, but showed significantly improved skin irritation degree, indicating hypoallergenicity.

&Lt; 110 > SK bioland Co., Ltd. <120> Cosmetic composition containing tangerine fermented broth with          감산산균 <130> YP-17-150 <160> 4 <170> KoPatentin 3.0 <210> 1 <211> 20 <212> DNA <213> Unknown <220> <223> Forward primer of Transglutaminase 1 <400> 1 tgggttacag aggcccaaga 20 <210> 2 <211> 21 <212> DNA <213> Unknown <220> <223> Reverse primer of Transglutaminase 1 <400> 2 ggcgcagtgt cactgtttca t 21 <210> 3 <211> 19 <212> DNA <213> Unknown <220> <223> Forward primer of actin <400> 3 ggcacccagc acaatgaag 19 <210> 4 <211> 21 <212> DNA <213> Unknown <220> <223> Reverse primer of actin <400> 4 ccgatccaca cggagtactt g 21

Claims (10)

A cosmetic composition comprising a fermentation broth obtained by fermenting citrus with lactic acid bacteria.
The method according to claim 1,
The lactic acid bacteria,
A cosmetic composition characterized by being a strain belonging to the genus Lactobacillus sp.
3. The method of claim 2,
The strain of the genus Lactobacillus,
A cosmetic composition characterized by being at least one strain selected from the group consisting of Lactobacillus plantarum , Lactobacillus casei and Lactobacillus paracasei .
The method according to claim 1,
The above-
Wherein the lactic acid bacteria are inoculated at a concentration of 1 x 10 &lt; ⁵ &gt; to 10 &lt; ⁷ &gt; cfu / ml and fermented at 15 to 45 DEG C for 1 to 15 days.
The method according to claim 1,
The citrus fruits,
Wherein the extract is an extract obtained by adding water to a juice of citrus pulp or citrus pulp.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for removing horny skin.
The method according to claim 6,
In the cosmetic composition,
Soap or a cleanser.
The method according to claim 1,
In the cosmetic composition,
Wherein the composition is for reinforcing skin barrier.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for skin cell regeneration.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for skin whitening.

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