KR20190053467A - Anti-aging composition comprising culture or its extract of microorganism of serratia genus - Google Patents

Abstract

Disclosed in the present specification are an anti-aging composition comprising Serratia sp. microorganisms, a crushed product thereof, a culture thereof or an extract of the culture as active ingredients; and Serratia sp. Dolsongi-HT4 microorganisms with accession number KCCM12140P. The composition and the microorganisms are effective in reducing skin wrinkles, improving skin elasticity, collagen synthesis, elastin synthesis, antioxidant activities, and inhibiting elastin degradation.

Description

TECHNICAL FIELD [0001] The present invention relates to an anti-aging composition comprising a culture of a microorganism belonging to the genus Serratia or an extract thereof as an active ingredient.

The term " Serratia " sp.) microorganism, a disruption thereof, a culture thereof or an extract of the above culture as an active ingredient.

There are many reasons for promoting aging, but Reactive Oxygen species (ROS) is considered to be one of the major causes. This active oxygen is indispensable for energy metabolism, immune response, etc., and is also an inevitable factor caused by external harmful environment. Active oxygen is highly reactive and causes a series of reactions in the body that cause DNA denaturation, induce excessive signal transduction, and protein denaturation, accumulating adverse health effects. These harmful reactions are supposed to preserve their homeostasis by antioxidants (uric acid, vit.C, vit.E etc.) or antioxidant enzymes (Glutathione peroxidase, superoxide dismutase, catalase, etc.) in vivo.

However, the aging of the antioxidant system due to endogenous aging and the accumulation of active oxygen by continuous noxious stimuli breaks this balance, harming health, promoting aging, causing various diseases such as skin diseases, skin cancer, arteriosclerosis and thrombosis do.

On the other hand, the collagen existing in the dermal layer of the skin occupies about 70 ~ 80% of the dry weight of the skin, and it is known to control skin elasticity together with elastic fiber elastin. MMP plays an important role in collagen degradation, which is involved in the degradation of extracellular matrix and basement membrane. The enzyme increases activity by aging or ultraviolet rays, and by inhibiting MMP, it is possible to reduce skin aging phenomena such as skin thickness increase and wrinkle formation induced by aging or ultraviolet rays.

Korean Patent Publication No. 10-2016-0097539

In one aspect, the present disclosure relates to the use of Serratia spp. It is an object of the present invention to provide a composition for anti-aging comprising microorganism, a disruption product thereof, a culture thereof or an extract of the culture as an active ingredient.

In another aspect, the present disclosure is directed to providing a Serratia sp. Dolsongi-HT4 microorganism having a deposit number of KCCM12140P.

In one aspect, the techniques disclosed herein are based on the genus Serratia sp.) microorganism, a disrupted product thereof, a culture thereof or an extract of the above culture as an active ingredient.

In an exemplary embodiment, the composition comprises (a) skin wrinkle improvement; (b) improving skin elasticity; (c) enhancing collagen synthesis; (d) Enhancing elastin synthesis; And (e) an elastin degradation inhibiting application.

In an exemplary embodiment, the composition may be one having antioxidant use.

In an exemplary embodiment, the composition may be one having DPPH radical scavenging activity.

In an exemplary embodiment, the microorganism may be Serratia sp. Dolsongi-HT4, which has the accession number KCCM12140P.

In an exemplary embodiment, the microorganism may be one having 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.

In an exemplary embodiment, the culture may be a filtrate of the culture or a culture supernatant in which the culture is centrifuged.

In one exemplary embodiment, the extract is one or more selected from the group consisting of a Hexane fraction, a Methylene Chloride fraction, an Ethyl Acetate fraction, a Butanol fraction, and a water fraction .

In an exemplary embodiment, the composition may be a cosmetic composition.

In another aspect, the technology disclosed herein provides a Serratia sp. Dolsongi-HT4 microorganism (Accession No. KCCM12140P).

In an exemplary embodiment, the microorganism may be one having 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.

In an exemplary embodiment, the microorganism may have anti-aging use.

In an exemplary embodiment, the anti-aging application comprises (a) improving skin wrinkles; (b) improving skin elasticity; (c) enhancing collagen synthesis; (d) Enhancing elastin synthesis; (e) inhibiting elastin degradation; And (f) an antioxidant activity application.

In one aspect, the techniques disclosed herein are based on the genus Serratia The present invention also provides an anti-aging composition comprising a microorganism, a disruption product thereof, a culture thereof or an extract of the culture as an active ingredient.

In another aspect, the technique disclosed herein is effective to provide a Serratia sp. Dolsongi-HT4 microorganism with accession number KCCM12140P.

Figure 1 shows the 16S rDNA partial sequence of Serratia sp. Dolsongi-HT4.
Fig. 2 shows the results of antioxidant activity test on cultures of Serratia sp. Dolsongi-HT4.
Figure 3 shows the results of antioxidant activity test for fractions of Serratia sp. Dolsongi-HT4 cultures of the genus Serratia .
Figure 4 shows the antioxidant activity test results for fractions of Serratia sp. Dolsongi-HT4 cultures.
Fig. 5 shows the results of analysis of expression of col1A1 and elastin gene by cultures of Serratia sp. Dolsongi-HT4 ( Serratia sp. Dolsongi-HT4).
FIG. 6 shows the results of analysis of the amount of procollagen I peptide synthesized by a culture of Serratia sp. Dolsongi-HT4 ( Serratia sp. Dolsongi-HT4).
Fig. 7 shows the results of the elastase inhibitory activity test of a culture of Serratia sp. Dolsongi-HT4 ( Serratia sp. Dolsongi-HT4).

Hereinafter, the present invention will be described in detail.

In one aspect, the techniques disclosed herein are based on the genus Serratia sp.) microorganism, a disrupted product thereof, a culture thereof or an extract of the above culture as an active ingredient.

In another aspect, the technology disclosed herein provides a Serratia sp. Dolsongi-HT4 microorganism (Accession No. KCCM12140P).

The Serratia species according to the present invention sp.) microorganisms have the effect of producing a substance having an anti-aging effect and providing an anti-aging use.

The microorganism according to the present specification may be improved or improved so as to have an activity equivalent to or higher than that by the conventional physicochemical mutation method or the like known in the art to which the present specification belongs.

As used herein, the term " active ingredient " refers to a component that can exhibit the desired activity alone, such as a carrier that exhibits the desired activity alone or is itself inactive.

As used herein, the term " anti-aging " means an application for preventing, delaying, and / or improving the aging phenomena caused by internal factors including genetic factors and external factors including ultraviolet rays and the like. For example, skin changes due to aging such as skin thickness increase, skin barrier damage, skin elasticity reduction, wrinkle formation, increase in depth or number of wrinkles, skin dryness, roughness, , Or to improve or alleviate it.

In an exemplary embodiment, the composition may be an anti-aging composition for photoaging.

In an exemplary embodiment, the composition may be an anti-aging composition that improves skin wrinkles or promotes skin elasticity. Specifically, the composition has the effect of preventing or preventing the generation of wrinkles by delaying the generation timing of wrinkles as much as possible, or alleviating or improving wrinkles that have already been generated. In addition, the composition has the effect of preventing or preventing skin elasticity reduction, or improving or improving skin elasticity.

In an exemplary embodiment, the composition may be one that inhibits collagen degradation or promotes collagen synthesis.

In an exemplary embodiment, the composition may be one that inhibits elastin degradation.

In an exemplary embodiment, the composition may be one that inhibits the expression of elastase or inhibits the activity of elastase that is minimally expressed by the compositions herein. In another aspect, the composition may also have specific effects on substances such as up-stream enzymes or proteins that inhibit the expression or activity of elastase.

As used herein, " antioxidant " means to ameliorate, treat and ameliorate the delayed use of the disease or condition causing the active oxygen species.

In an exemplary embodiment, the composition may be one having DPPH radical scavenging activity.

The crushed product may mean a product obtained by crushing the microorganism itself by chemical or physical force.

The culture may refer to a substance including some or all of the substances contained in the medium in which the microorganism has been cultured, regardless of the form of the culture. For example, a substance containing metabolites or secretions resulting from the microbial culture or a fragment thereof , And the microorganism itself may be contained in the culture.

In an exemplary embodiment, the culture may be a culture medium itself in which the microorganism according to the present invention is cultured in a suitable culture medium, a filtrate (filtrate or centrifuged supernatant) from which the microorganism has been removed by filtration or centrifugation of the culture medium, Or a cell lysate obtained by treating the culture broth with a lysozyme, but the present invention is not limited thereto. In one aspect, it may be preferred that the culture is a culture supernatant obtained by centrifuging the filtrate or culture of the culture, but not the material obtained by breaking down the cells.

The extract may be a product obtained by extracting the culture regardless of the extraction method, the extraction solvent, the extracted component or the form of the extract, and includes all substances that can be obtained by processing or treating with other methods after extraction Is a broad concept.

In one exemplary embodiment, the extract is a hexane fraction, a methylene chloride fraction, a hexane fraction, and a hexane fraction obtained by sequentially fractionating in the order of hexane, methylene chloride, ethyl acetate and butanol, Ethyl acetate fraction, and butanol fraction.

In an exemplary embodiment, the extract may be a water fraction.

In an exemplary embodiment, the active ingredient may be included in an amount of 0.001 to 10% by weight based on the total weight of the composition.

In an exemplary embodiment, the composition may be a cosmetic composition.

The cosmetic composition may further contain, in addition to the active ingredient, a functional additive and a component included in a general cosmetic composition. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, powder, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, shampoo , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser, but the present invention is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, have.

According to one exemplary embodiment, the composition may be a pharmaceutical composition.

The pharmaceutical composition may further contain, in addition to the active ingredient, a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure, and other therapeutically useful substances, And can be formulated into various oral or parenteral dosage forms accordingly.

Examples of the oral administration agent include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, powders, powders, granules, granules and pellets, , Diluents (such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (such as silica, talc, stearic acid and magnesium or calcium salts thereof and polyethylene glycols) . The tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. The tablets may be prepared by conventional mixing, granulating or coating methods.

In addition, the parenteral administration forms may be transdermal dosage forms, and may be formulations such as injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, But is not limited thereto.

The dosage of the active ingredient is within the level of ordinary skill in the art and the daily dose of the drug depends on various factors such as the degree of progress of the subject to be administered, the age of onset, age, health condition, The composition may be administered in an amount of 1 / / kg to 200 mg / kg in one aspect, and 50 / / kg to 50 mg / kg in another aspect, divided into 1 to 3 times a day, Are not intended to limit the scope of the invention in any way.

The pharmaceutical composition may be an external preparation for skin, and the external preparation for skin may include any substance applied outside the skin, and may include various formulations of medicines.

According to one exemplary embodiment, the composition may be a food composition.

The food composition may be in a liquid or solid form and may be in the form of, for example, various foods, beverages, gums, tea, vitamin complexes, health supplements and the like and may be in the form of powders, granules, tablets, capsules or beverages . The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients.

There are no particular limitations on the liquid components that can be contained in addition to the active ingredients disclosed in this specification, and may include various flavoring agents or natural carbohydrates such as ordinary beverages as additional ingredients. Examples of the natural carbohydrate include common saccharides such as disaccharides such as monosaccharide, glucose and fructose, polysaccharides such as maltose and sucrose, dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol . As the above flavorings, natural flavors (e.g., tau martin, stevia extract, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (e.g., saccharin, aspartame, etc.) . The ratio of the natural carbohydrate may be generally about 1 to 20 g, and in one aspect about 5 to 12 g per 100 ml of the composition disclosed herein.

In one aspect, the food composition may include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate, etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. On the other hand, flesh for the production of natural fruit juices and vegetable beverages. These components can be used independently or in combination. The proportion of the additive may vary, but is generally selected in the range of from 0.001 to about 20 parts by weight per 100 parts by weight of the composition disclosed herein.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Test Example 1. Isolation of microorganisms having antioxidative activity

The microorganisms having antioxidant activity were isolated from the ground water of the stone paddy field in Jeju Island, Korea as follows.

Microorganisms obtained from water sources are plated on LB (Luria-Bertani), Nutrient Broth (NB), Brain Heart Infusion (BHI) and Yeast extract-peptone-Dextrose (YPD) Lt; / RTI > incubator. After culturing, various microorganisms grown in solid medium were cultured in liquid medium (3 ml) for 24 hours, and then the cells were removed through a centrifuge to obtain a culture solution. Antioxidant activity was measured by DPPH assay.

The microorganisms were cultured as described above, and microorganisms having high antioxidative activity could be selected through the DPPH assay. DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals dissolved in organic solvents have the maximum absorbance at 515 nm. Antioxidants dissolve DPPH radicals and lose their intrinsic color and change their transparency.

Herein, the DPPH assay is as follows. 100 μM of DPPH (2,2-diphenyl-1-picrylhydrazyl) and samples to be treated were prepared, and the prepared samples were mixed at a ratio of 1:20 with DPPH solution and stored at 37 ° C. for 30 minutes. After that, the absorbance of the reacted sample was measured at 517 nm using a spectrophotometer. The lower the absorbance, the higher the antioxidant power.

Test Example  2. Identification of isolated microorganisms

In order to identify the microorganism having high antioxidative activity, which was isolated in Test Example 1, a DNA sequence encoding the 16S ribosomal RNA partial sequence of the microorganism was analyzed.

After the microorganisms were cultured in a liquid medium, genomic DNA was extracted from the microorganisms to obtain 27F (5'-AGAGTTTGATCMTGGCTCAG-3 ', SEQ ID NO: 2) / 1492R (5'-TACGGYTACCTTGTTACGACTT-3' 3) amplification by PCR (polymerase chain reaction) method using primers and its sequence was analyzed.

Sequence analysis showed that Serratia sp. And 99% homology to the 16S ribosomal RNA partial sequence of Se40.

Thus, the novel microorganism strains isolated and cultivated in this specification were confirmed to be Serratia sp. By molecular system taxonomic analysis based on the 16S rDNA nucleotide sequence, and it was confirmed that Serratia sp Dolsongi-HT4), and the novel microorganism strain was deposited on October 26, 2017 with the deposit number KCCM12140P in the Korean Society for Microbiological Care, which is a depository institution. The partial sequence of Serratia sp. Dolsongi-HT4 16S rDNA of SEQ ID NO: 1 is shown in Fig.

Experimental Example 3: Streptomyces sp. Serratia  sp. Antioxidant activity of Dolsongi-HT4) culture

The microorganisms identified in Test Example 2 were dissolved in M9 medium (CaCl ₂ 0.015 g / L, Na ₂ HPO ₄ 6.78 g / L, KH ₂ PO ₄ 3 g / L, NaCl 0.5 g / L, NH ₄ Cl 1 g / L, MgSO ₄ 0.5 g / L, Glucose 1% (w / v)) for 15 hours and then the culture was centrifuged at 5,000 rpm for 10 minutes to obtain a supernatant. The obtained supernatant was assayed for antioxidative activity by DPPH assay.

As a result, as shown in FIG. 2, the cultures were compared with samples (AA-5, AA-2.5, AA-1.25) containing ascorbic acid at a concentration of 1.25 to 5 ppm It was confirmed that similar effects or remarkably excellent effects were obtained.

Test Example 4. Streptomyces sp. Serratia  sp. Antioxidant activity of fractions from Dolsongi-HT4) cultures 1

(N-Hexane (Hex), Methylene Chloride (MC), Ethyl Acetate (EA)) from the culture supernatant obtained by centrifuging the culture to remove the microorganism from the culture obtained in Test Example 3, , n-Butanol (BuOH)), fractions of Hex, MC, EA and BuOH were sequentially prepared.

Concretely, the same amount (1: 1 (v / v)) of the solvent as the sample was mixed, and the mixture was allowed to stand for about 3 hours to separate the layers. The water fraction and the solvent fraction of the layered sample were separated, and the water fraction was mixed with the same amount of the following solvent in the same manner. The above procedure was repeated to obtain a total of five fractions (Hex, MC, EA, BuOH , water). The separated fractions were evaporated using a rotary evaporator and concentrated to dryness. The DPPH assay was used to compare the antioxidant efficacy of the prepared samples.

As a result, as shown in FIG. 3, the fractions of the supernatant of the culture contained samples (AA-10, AA-5, AA-2.5) containing ascorbic acid at a concentration of 2.5 to 10 ppm, It was confirmed that similar effects or remarkably excellent effects were obtained. In particular, it was found that a large amount of antioxidant substances were contained in the methylene chloride fraction and the water fraction as an aqueous solution layer obtained after fractionation of the organic solvent from the culture supernatant.

Experimental Example 5 [0060] Serratia  sp. Antioxidant activity of fractions from Dolsongi-HT4) cultures 2

The culture obtained in Test Example 3 was fractionated using an ethyl acetate solvent, and then separated and purified using a C18 column. Then, the organic solvent was removed, and the following antioxidant activity was measured on the dried sample.

Specifically, a DPPH (1-diphenyl-2-picryl-hydazil) free radical scavenging assay was performed by mixing 0.25 ml of DPPH solution (0.1 mM of DPPH in methanol) with 0.75 ml of various concentrations of test samples . The mixture was incubated at room temperature for 3 hours and the absorbance was measured at 517 nm using an ultraviolet-visible spectrophotometer (SCINCO). A reaction mixture containing no sample or mixed with commercially available butylated hydroxyanisole (BHA) was used as a negative and positive controls, respectively, To calculate the standard deviation.

As a result, it was confirmed that the fraction obtained from the culture had a very strong antioxidant ability with an IC ₅₀ value of 1.35 / / ml in terms of DPPH free radical scavenging activity (see FIG. 4).

Experimental Example 6 [0060] Serratia  sp. Effect of Fractions from Dolsongi-HT4 Cultures on Skin Matrix Proteins

The effect of the fraction obtained from the culture obtained in Test Example 4, specifically methylene chloride fraction, on the amount of expression of collagen (Col1A1), elastin gene expression and the amount of procollagen type I in fibroblast was examined. Collagen is synthesized in the cells as procollagen, secreted extracellularly and polymerized into collagen fibers, wherein the N-terminal and C-terminal propeptides of the procollagen are released by endopeptidase. In this test, PIP was quantified (unit: ng / ml) using human procollagen I peptide (PIP) kit.

Specifically, NHDF (Normal Human Dermal Fibroblast) cells were cultured on a 6-well plate at 4 × 10 ⁵ cells / well. After 24 hours, UVB 50 ml / cm ² was irradiated and the fractions were diluted in serum-free media and treated in each well. Twenty-four hours later, Trizol was used to dissolve the cells. Total RNA was extracted and transformed into cDNA. The expression of colA1 and elastin gene was confirmed by qPCR.

As a result, it was confirmed that irradiation of UVB 50 mJ / cm ² with NHDF (Normal Human Dermal Fibroblast) cells increased the amount of reduced col1A1 and elastin gene expression by 100% or more when the fraction was treated at 2 μg / ml or more Reference). In addition, it was confirmed that the amount of procollagen type I synthesized was increased by 100% or more when the fraction was treated at 2 μg / ml or more (see FIG. 6).

Test Example 7: Serratia  sp. Effect of Fractions from Dolsongi-HT4 Cultures on Skin Matrix Degrading Enzymes

The effect of the fraction obtained from the culture obtained in Test Example 4, specifically the methylene chloride fraction, on elastase activity was confirmed. Elastase is an enzyme that degrades elastin, one of the proteins that form a network structure that regulates the elasticity of the skin in the dermal tissue of the skin. Therefore, inhibition of the activity of this enzyme can inhibit skin aging.

50 μl of 0.4 M Tris-HCl buffer (pH 8.6), 50 μl of sample, and 50 μl of 0.6 U / ml elastase diluted in the same buffer were added and reacted at 37 ° C for 30 minutes. Subsequently, 100 μl of the substrate (N-succinyl-Ala-Ala-Ala-p-nitroanilide) was added and reacted at 37 ° C. for 30 minutes. Absorbance was measured at 410 nm.

As a result, as shown in FIG. 7, 25% at the concentration of 200 μg / ml, 32% at the concentration of 500 μg / ml and 35% of the inhibitory activity of the elastase at the concentration of 1000 μg / ml Respectively.

Formulation examples of compositions according to one aspect of the present disclosure are described below, but are applicable to various other formulations, which are not intended to be limiting, but rather to illustrate only specifically.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) The culture of Test Example 3 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) The culture of Test Example 3 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Compounding ingredient Content (% by weight) The culture of Test Example 3 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Compounding ingredient Content (% by weight) The culture of Test Example 3 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Compounding ingredient Content (% by weight) The culture of Test Example 3 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative Suitable amount Preservative, coloring, fragrance Suitable amount Purified water Balance

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Korea Microorganism Conservation Center KCCM12140P 20171026

<110> AMOREPACIFIC CORPORATION <120> ANTI-AGING COMPOSITION COMPRISING CULTURE OR ITS EXTRACT OF          MICROORGANISM OF SERRATIA GENUS <130> 17P558 / IND <160> 3 <170> KoPatentin 3.0 <210> 1 <211> 1153 <212> DNA <213> Unknown <220> <223> Serratia sp. Dolsongi-HT4 <400> 1 tgtgggggcg gctaccatgc agtcgagcgg tagcacaggg agcttgctcc tgggtgacga 60 gcggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa 120 acggtagcta ataccgcata acgtctacgg accaaagtgg gggaccttcg ggcctcatgc 180 catcagatgt gcccagatgg gattagctag taggtggggt aatggctcac ctaggcgacg 240 atccctagct ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc 300 ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc 360 gcgtgtgtga agaaggcctt cgggttgtaa agcactttca gcgaggagga agggtactgt 420 gttaatagca cagtgcattg acgttactcg cagaagaagc accggctaac tccgtgccag 480 cagccgcggt aatacggagg gtgcaagcgt taatcggaat tactgggcgt aaagcgcacg 540 caggcggttt gttaagtcag atgtgaaatc cccgcgctta acgtgggaac tgcatttgaa 600 actggcaagc tagagtcttg tagagggggg tagaattcca ggtgtagcgg tgaaatgcgt 660 agagatctgg aggaataccg gtggcgaagg cggccccctg gacaaagact gacgctcagg 720 tgcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgct gtaaacgatg 780 tcgacttgga ggttgtgccc ttgaggcgtg gcttccggag ctaacgcgtt aagtcgaccg 840 cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctactct tgacatccag 960 agaattcgct agagatagct tagtgccttc gggaactctg agacaggtgc tgcatggctg 1020 tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct 1080 ttgttgccag cacgtaatgg tgggaactca aaggagactg ccggtgataa accggaggaa 1140 ggtggggatg aca 1153 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F primer <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R primer <400> 3 tacggytacc ttgttacgac tt 22

Claims (13)

A composition for anti-aging comprising a microorganism of the genus Serratia sp., A fragment thereof, a culture thereof or an extract of the culture as an active ingredient.
The method according to claim 1,
Wherein the composition has one or more of the following uses.
(a) improving skin wrinkles;
(b) improving skin elasticity;
(c) enhancing collagen synthesis;
(d) Enhancing elastin synthesis; And
(e) inhibition of elastin degradation.
The method according to claim 1,
Wherein said composition has antioxidant use.
The method of claim 3,
Wherein the composition has DPPH radical scavenging activity.
The method according to claim 1,
Wherein said microorganism is Serratia sp. Dolsongi-HT4 with accession number KCCM12140P.
6. The method of claim 5,
Wherein the microorganism has 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.
The method according to claim 1,
Wherein the culture is a culture supernatant obtained by centrifuging a filtrate or a culture of the culture.
The method according to claim 1,
Wherein the extract is at least one selected from the group consisting of Hexane fraction, Methylene Chloride fraction, Ethyl Acetate fraction, Butanol fraction and Water fraction.
The method according to claim 1,
Wherein the composition is a cosmetic composition.
Serratia genus Stone cluster - HT4 ( Serratia sp. Dolsongi-HT4) microorganism (Accession No .: KCCM12140P).
11. The method of claim 10,
Wherein said microorganism has a 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.
11. The method of claim 10,
Wherein said microorganism has anti-aging use.
13. The method of claim 12,
Wherein the anti-aging application has one or more of the following uses.
(a) improving skin wrinkles;
(b) improving skin elasticity;
(c) enhancing collagen synthesis;
(d) Enhancing elastin synthesis;
(e) inhibiting elastin degradation; And
(f) Antioxidant activity.

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