KR20190046218A - Genetically modified tomato plants with a novel growth pattern - Google Patents

Abstract

The present invention relates to a genetically modified tomato plant with an increased yield of tomato, which comprises at least one base sequence of SEQ ID NO: 1 and SEQ ID NO: 2.

Description

The present invention relates to a novel genetically modified tomato plant with a novel growth pattern,

The present invention relates to a novel genetically modified tomato plant with a growth aspect, and more preferably to a tomato plant which can be cultivated by a hanging-bud type cultivation method.

Tomatoes (Solanum Lycopersicum or Lycopersicon Esculentum) are crops containing a variety of vitamins and lycopene, which are considered healthy vegetables. Tomatoes are one of the most consumed vegetables in the world, and the popularity of tomatoes is continuously increasing in Korea. In order to effectively cope with the increase in demand for tomatoes, it is necessary to improve the cultivation method of tomato plants.

The categories of plant cultivation methods are divided into non-cultivation and cultivation. Noi cultivation means outdoor cultivation utilizing natural climate change and precipitation. There is no need to invest in facilities and the number of plants per plant is relatively large. However, the cultivation of the soil has a decisive disadvantage that it is difficult to control the amount of water. Plant cultivation, on the other hand, means planting plants in a facility where temperature and humidity remain constant. In case of facility cultivation, it is advantageous to obtain uniform quality products by regulating cultivation environment constantly as opposed to cultivating novi. However, there is a drawback that the cultivation area of the facility is narrow compared to the investment cost of the facility compared with the cultivation of the non-cultivated area.

In the case of tomato plants, the stem does not grow straight up due to its growth characteristics. Therefore, in order to increase the yield of the tomato, the supporting rod or the man-in line should be installed separately and fixed so that the stem can continue to grow up. When cultivating the facility, it is usually preferable to install a rail or a braiding wire on the ceiling of the facility and to send down the tow line from the rail or braiding wire.

However, it takes a lot of work force to fix the tomato plants by utilizing the manned ropes. It is necessary to fix the stem of the plant to the attraction line and remove unnecessary side line in accordance with the growth of the tomato plant. If the above work is not accompanied by the cultivation process, there is a risk that the yield of the tomato itself will be reduced if the tomato quality is not obtained uniformly or if it is severe.

Conventionally, in the cultivation of tomato plants, the troublesome work as described above was solved by labor input. In recent years, however, aging of the rural population has intensified and the absolute number of laborers has been reduced, making it difficult to meet the growing demand for tomatoes with labor input alone. Therefore, it is urgent to develop tomato plants and their cultivation methods that can reduce labor force required for cultivation of tomato plants.

US registered patent US 5,333,409

In order to solve the above-mentioned problems, the present invention aims to provide a tomato plant with reduced labor required for cultivation and increased yield. The aim is to provide a tomato plant which is grown in a different manner, preferably as a genetically modified plant, with reduced labor required for cultivation and increased yield.

In order to achieve the above object, the present invention discloses a genetically modified tomato plant comprising a specific base sequence. The genetically modified tomato plant of the present invention comprises the nucleotide sequence of SEQ ID NO: 1 or the nucleotide sequence of SEQ ID NO: 2.

In addition, the genetically modified tomato plant of the present invention may contain a base sequence in which the 299th base in the nucleotide sequence of SEQ ID NO: 3 is substituted with C to T. The nucleotide sequence in which the 299th base in the nucleotide sequence of SEQ ID NO: 3 is substituted with C to T is the same as the nucleotide sequence of SEQ ID NO:

In addition, the genetically modified tomato plant of the present invention may contain a nucleotide sequence in which the 676th base in the nucleotide sequence of SEQ ID NO: 4 is substituted with A to T. The base sequence in which the 676th base in the nucleotide sequence of SEQ ID NO: 4 is replaced by A to T is the same as the nucleotide sequence of SEQ ID NO:

In addition, the genetically modified tomato plant of the present invention may be a tomato plant comprising a base sequence encoding the polypeptide of SEQ ID NO: 5, wherein the genetically modified tomato plant of the present invention has the sequence identity of SEQ ID NO: 6 Lt; RTI ID = 0.0 > a < / RTI > base sequence encoding a polypeptide.

Preferably, the nucleotide sequence encoding the polypeptide of SEQ ID NO: 5 is the same as the nucleotide sequence of SEQ ID NO: 1, or may be a form in which some nucleotides of SEQ ID NO: 1 are substituted. The nucleotide sequence encoding the polypeptide of SEQ ID NO: 6 may be the same as or different from the nucleotide sequence of SEQ ID NO: 2, or the nucleotide sequence of SEQ ID NO: 2.

The present invention also encompasses a tomato plant wherein the nucleotide sequence of SEQ ID NO: 1 is a homozygous sequence and the nucleotide sequence of SEQ ID NO: 2 is a homozygous junction. In addition, the present invention provides a tomato plant wherein the base sequence of SEQ ID NO: 1 is a homozygous sequence and the base sequence of SEQ ID NO: 2 is a heterozygous sequence or a base sequence of SEQ ID NO: 1 is a heterozygous sequence, Tomato plants whose nucleotide sequences are homozygous. The present invention also encompasses tomato plants wherein the base sequence of SEQ ID NO: 1 is a heterozygous sequence and the base sequence of SEQ ID NO: 2 is a heterozygous sequence.

Thus, the genetically modified tomato plant of the present invention may be submerged against SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2. The genetically modified tomato plant of the present invention may also be an inbreeding hybrid for SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2. In addition, the genetically modified tomato plant of the present invention may be a hybrid for SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2.

In order to achieve the above-mentioned object, the genetically modified tomato plant of the present invention shows different stem growth pattern from the existing tomato plant. As a result, the hatching-type cultivation method can be applied to the tomato plant of the present invention. By applying the hanging-bud type cultivation method, labor force required for cultivation of tomato plant is reduced and yield of tomato is increased.

FIGS. 1A to 1B are schematic diagrams comparing the conventional tomato plant cultivation method and the genetically modified tomato plant cultivation method of the present invention.
Figures 2a-2f compare growth patterns, inflorescence, and loss of tomato plants between the existing tomato plants and genetically modified tomato plants of the present invention.
Figure 3 conceptually shows the mutation positions of the nucleotide sequences of SEQ ID NO: 1 contained in genetically modified tomato plants of the present invention.
Fig. 4 conceptually shows the mutation position of the nucleotide sequence of SEQ ID NO: 2 contained in the genetically modified tomato plant of the present invention.
Figures 5A and 5B compare the nucleotide sequence of the wild type cDNA with the nucleotide sequence of SEQ ID NO:
FIGS. 6A to 6C show the nucleotide sequences of the wild type cDNA and the nucleotide sequences of SEQ ID NO: 2.
7 compares the polypeptide sequence of the wild type polypeptide with the polypeptide sequence of SEQ ID NO: 5.
Figure 8 compares the polypeptide sequence of the wild type polypeptide with the polypeptide sequence of SEQ ID NO: 6.
Figure 9 is a simplified representation of the manner in which the genetically modified tomato plants of the present invention are obtained.

The invention disclosed herein relates to a mutation in a plant of a branch, preferably a tomato plant. Tomato plants containing novel genetic mutations have different characteristics of cultivation methods compared to existing tomato plants and have more favorable characteristics in terms of labor saving and efficient cultivation, management and harvesting.

Harvesting of the tomato plant means obtaining the product from the tomato plant, preferably the product may be tomato. Therefore, it is appropriate that the contents described in the following description of tomatoes include reference to the generic product of the tomato plant rather than simply tomatoes.

Tomato plants are largely classified into two types, ie, non-cultivation and plant cultivation. For the proper growth of tomato plants, it is necessary to fix the stem of the tomato plant, and this necessity is effective for both non-cultivation and cultivation. In the case of cultivation in the field, a separate support stand is set up and the tomato stem is fixed by installing a mesh or manned line connecting the struts. On the other hand, in the case of plant cultivation, there is a case where a support stand is set up, but generally, a method of fixing a tomato plant by installing rails or braiding wires in the inside of the facility and installing the manned line downward from the rail or wire Is preferred.

The excitation is largely divided into the change of the growth direction of the plant by the external stimulus, and it is divided according to the kind of the stimulus circle in detail. If the stimulus circle is a light, it is a phototropism. If the stimulus circle is a gravity, it is a gravitropism. If the stimulus circle is a humidity, it is a hydrotropism. If the stimulus circle is a chemical substance, Chemotrophism), the stimulus source is physical contact (Thugmotropism), and if the stimulus source is injured, it is called osteomorphism (traumatropism).

On the other hand, positive irritability means that the plant grows in a direction in which the external stimulus is applied, and negative irritation means that the plant grows in a direction away from the external stimulus. For example, positive roughening means that growth takes place in the direction of gravity, and voice roughening means that growth takes place in the opposite direction of gravity.

It is known that Auxin plays an important role in the manifestation of various kinds of excitations, especially in terms of luminosity and opacity. Auxin is a kind of plant growth hormone, which is involved in the growth of the stem. It is well known that oxine tends to move away from the direction in which the external light stimulus is given and is localized on the side where no light stimulus is given. Conversely, in the case of gravity, auxin tends to be attracted in the direction of gravity, and as a result, tends to be localized at the bottom of the stem of the plant. Influx carriers and efflux carriers intervene in the transport of auxin.

Polypeptides encoded by the LAZY1 wild-type sequence are known to interfere with Polar Auxin Transports and control the distribution of auxin in the stem tissues of plants. Since auxin is required to be asymmetrically distributed in the lower part of the plant stem in order for the vigorous expression to be expressed, the polar distribution of auxin through the expression of the function of the LAZY1 polypeptide is an essential condition for the stem of the plant to exhibit negative actinic activity.

On the other hand, the polypeptide expressed by the LAZY1-mutant nucleotide sequence is a form in which the asymmetric regulation of the polarity distribution of the auxin is inhibited. When the LAZY1-mutant polypeptide is expressed, the distribution of the auxin is not maintained asymmetrically. Thus, the length of the plant stem grows throughout the plant stem, not the bottom of the stem asymmetrically. Asymmetric growth is excluded, and gravity, which can be regarded as an external force, is the most important factor determining the growth direction of the plant stem. As a result, the stem of the plant exhibits a positive agonism.

The mechanism by which the LAZY1-mutant nucleotide sequence is expressed and the stem of the plant exhibits a positive activity is not limited to tomato plants. The above mechanism is also applicable to plants that inherit the LAZY1 wild-type sequence from the common ancestor and have homology with the tomato plant in the nucleotide sequence. For example, the LAZY1 sequence of rice (Oryza sativa) and Arabidopsis thaliana is homologous to the tomato plant.

The SCR nucleotide sequence is an important nucleotide sequence that determines the differentiation patterns of the endothelial and cortical layers of plant roots and stems. Typically, the endothelia of plant stems contain statoliths, which participate in the gravitational orientation of the plant. In order for the phylogeny of the plant stem to be expressed, the process of recognizing the direction of gravity must be preceded, so that if there is a problem in the direction of the gravity direction of the plant, the voice actinism may not be expressed.

Tomato plants containing the SCR-mutant base sequence are unable to synthesize polypeptides capable of expressing the function required during endothelial development due to deletion of excess base on the SCR nucleotide sequence. Therefore, tomato plants containing the above-mentioned SCR-mutant base sequence are defective in endothelial development, and there is a problem in recognition of the gravitational direction. As a result, since the osseous segregation can not be achieved corresponding to the direction of gravity, continuous pressure due to gravity is a factor that determines the growth pattern of the plant stem.

Hereinafter, the present invention will be described in more detail with reference to examples and drawings of the present invention. It is to be understood, however, that such description is provided to enable those of ordinary skill in the art to understand the present invention properly, and that the embodiments of the present invention may be modified into various other forms. It is to be understood that the scope of the present invention is not limited to the drawings and examples described below, but is only determined by what is described in the claims.

The genetically modified tomato plant of the present invention comprises the nucleotide sequence of SEQ ID NO: 1 or the nucleotide sequence of SEQ ID NO: 2. The nucleotide sequence of SEQ ID NO: 1 is otherwise referred to as the LAZY1-mutant and the nucleotide sequence of SEQ ID NO: 2 is otherwise referred to as the SCR-mutant .

In addition, the genetically modified tomato plant of the present invention may have a nucleotide sequence in which the 299th base in the nucleotide sequence of SEQ ID NO: 3 is substituted with C to T, and the nucleotide sequence of SEQ ID NO: 3 is LAZY1 -Wild type . The base sequence in which the 299th base of LAZY1-Wild type is replaced by C to T is the same as the LAZY1-mutant of SEQ ID NO:

Meanwhile, the genetically modified tomato plant of the present invention may have a nucleotide sequence in which the 676th base in the nucleotide sequence of SEQ ID NO: 4 is substituted with A to T, and the nucleotide sequence of SEQ ID NO: 4 is SCR -Wild type . The nucleotide sequence in which the 676th nucleotide of SCR-Wild type is replaced by A to T is the same as the SCR-mutant of SEQ ID NO:

In addition, the genetically modified tomato plant of the present invention may be a tomato plant comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO: 5. The polypeptide of SEQ ID NO: 5 is otherwise referred to as a LAZY1-mutant polypeptide . The nucleotide sequence encoding the polypeptide of SEQ ID NO: 5 may be the nucleotide sequence of SEQ ID NO: 1 or the nucleotide sequence of SEQ ID NO: 1.

The genetically modified tomato plant of the present invention may be a tomato plant comprising the nucleotide sequence encoding the polypeptide of SEQ ID NO: 6. The polypeptide of SEQ ID NO: 6 is otherwise referred to as the SCR-mutant polypeptide . The nucleotide sequence encoding the polypeptide of SEQ ID NO: 6 may be the nucleotide sequence of SEQ ID NO: 2, or may be a form in which some nucleotides of the nucleotide sequence of SEQ ID NO: 2 are substituted.

The present invention also encompasses a tomato plant wherein the nucleotide sequence of SEQ ID NO: 1 is a homozygous sequence and the nucleotide sequence of SEQ ID NO: 2 is a homozygous junction. In addition, the present invention provides a tomato plant wherein the base sequence of SEQ ID NO: 1 is a homozygous sequence and the base sequence of SEQ ID NO: 2 is a heterozygous sequence or a base sequence of SEQ ID NO: 1 is a heterozygous sequence, Tomato plants whose nucleotide sequences are homozygous. The present invention also encompasses tomato plants wherein the base sequence of SEQ ID NO: 1 is a heterozygous sequence and the base sequence of SEQ ID NO: 2 is a heterozygous sequence.

Thus, the genetically modified tomato plant of the present invention may be submerged against SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2. The genetically modified tomato plant of the present invention may also be an inbreeding hybrid for SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2. In addition, the genetically modified tomato plant of the present invention may be a hybrid for SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 1 and SEQ ID NO: 2.

In addition, it is assumed that the branches and plants disclosed in the present invention include a tomato plant as well as a base sequence which is homologous with the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2 Solanum melongena, Solanum tuberosum Solanaceae Nicotiana rice (Oryza sativa), Arabidopsis thaliana, and the like. The homologous sequence includes LAZY1 homologs or SCR homologs, and Homologs generally refers to several species in which the corresponding nucleotide sequence ( LAZY 1 nucleotide sequence or SCR nucleotide sequence ) is derived from a common ancestor. Therefore, although the present invention mainly discloses a tomato plant, the technical content of the present invention is not limited to a tomato plant, but may be a plant in which all or some of the branches and branches and the homology of the two base sequences are recognized Can be expanded.

FIGS. 1A to 1B are schematic diagrams comparing the conventional tomato plant cultivation method and the genetically modified tomato plant cultivation method of the present invention. The stem of the existing tomato plant had a negative response. On the other hand, the stem of the genetically modified tomato plant disclosed in the present invention has a positive roughening property. Figs. 1A to 1B schematically show that the aspects of the cultivation method differ depending on the difference in the positive and negative activity.

FIG. 1A depicts a method of cultivating an existing tomato plant based on facility cultivation. When the existing tomato plants were seeded with the seedlings of the existing tomato plants, the stem was grown in the opposite direction of gravity. Therefore, in order to assist the growth, it was necessary to install an attracted string (or attracted string). During the cultivation of the facility, strong wires or rails were installed on the upper part to connect the above manned lines.

Figure 1b, on the other hand, depicts the manner in which the genetically modified tomato plants of the present invention are grown. The method shown in FIG. 1B is called a hanging bed type cultivation method. The stem of the genetically modified tomato plant of the present invention grows in the direction of gravity because it has a negative rogue. This gives the impression that the stem is lying or sagging. The roots of roots of tomato plants have the same positive roots as the existing tomato plants. Therefore, it is not necessary to develop a separate container to contain the seedling seedling.

It is sufficient if the soil for the genetically modified tomato plant of the present invention is planted on one side of the container. Thus, FIG. 1B depicts the top of the soil contained in the container being exposed, so that the stem of the genetically modified tomato plant is stretched from the top of the container. However, the exposure direction of the tomato plant must be the upper It does not. For example, a cultivation method in which some soil is revealed to the underside of a container and the genetically modified tomato plant of the present invention is planted in the soil exposed to the underside is also included in the present invention.

The stem of a genetically modified tomato plant of the present invention exhibits negative sonographicity. As shown in FIG. 1B, such characteristics can be achieved by simultaneously planting several seedlings at different heights in one space, which means that the efficiency of cultivation of tomato plants is increased. Therefore, the tomato yield per unit area can be increased by growing the genetically modified tomato plant of the present invention. It is also an important advantage of the present invention that the cultivation environment suitable for the physical condition of the grower can be created by varying the height at which the seedlings are planted.

Figures 2a-2f compare growth patterns, inflorescence, and loss of tomato plants between the existing tomato plants and genetically modified tomato plants of the present invention. Figures 2a and 2d are photographs of the rooting and inflorescence and deletion of the wild-type tomato plant stem. FIGS. 2B and 2E are photographs of stem profiling and inflorescence and deletion of genetically modified tomato plants containing the LAZY1-mutant base sequence. FIGS. 2c and 2f are photographs of the stem roots and inflorescence and deletion of genetically modified tomato plants containing the SCR-mutant base sequence.

Referring to FIG. 2A, it can be seen that the stem of the wild-type tomato plant exhibits negative actinic activity. On the other hand, referring to FIGS. 2b and 2c, it can be seen that the stem of the genetically modified tomato plant including the LAZY1-mutant or the SCR-mutant exhibits positive agonism.

It should also be noted that a genetically modified tomato plant comprising the LAZY1-mutant or SCR-mutant is only different in stalacticity from the existing tomato plant, and the other characteristics remain intact. In particular, referring to Figures 2d to 2f, it can be seen that the inflorescence and fruiting aspect are the same in the tomato plant (see Figure 2d) and the genetically modified tomato plant of the present invention (see Figure 2e and Figure 2f) have. Therefore, when comparing single tomato plants, it can be expected that tomato yield will be kept at least equal.

Figure 3 conceptually shows the mutation positions of the nucleotide sequences of SEQ ID NO: 1 contained in genetically modified tomato plants of the present invention. The nucleotide sequence of SEQ ID NO: 1 is otherwise LAZY1-mutant . LAZY1-mutant is a nucleotide sequence in which the 299th base of the gene coding for the LAZY1 - Wild type in tomato chromosome 5 is replaced by C to T.

In addition, Figure 3 is in the 5 chromosome of tomato, LAZY1 - The nucleotide sequence of Wild type and location LAZY1 - shows the configuration of a Wild type. In the structure at the bottom of the figure , a thick line indicates an exon, a thin line indicates an intron, and a LAZY1- Wild type and a LAZY1-mutant include six major ones .

Figure 4 conceptually shows the mutation positions of the nucleotide sequences of SEQ ID NO: 2 contained in genetically modified tomato plants of the present invention. The nucleotide sequence of SEQ ID NO: 2 is otherwise SCR-mutant . SCR is an abbreviation of SCARECROW. The SCR-mutant is a nucleotide sequence in which the 676th nucleotide of the SCR - wild type coding sequence located in chromosome 10 of tomato is replaced by A to T.

In addition, Figure 4 in the chromosome 10 of the tomato, SCR - The nucleotide sequence of Wild type indicates the location and configuration of the SCR-type Wild. In the structure at the bottom of the figure, the bold line indicates the exon, and the thin line indicates the intron. The SCR - Wild type and the SCR-mutant include two axons.

Figures 5A and 5B compare the nucleotide sequence of the wild type cDNA with the nucleotide sequence of SEQ ID NO: The wild-type cDNA base sequence is the same as the nucleotide sequence of SEQ ID NO: 3. Referring to FIG. 5A, it can be confirmed that the nucleotide sequence of SEQ ID NO: 1 is different from the nucleotide sequence of SEQ ID NO: 3 only in the 299th nucleotide and all of the remaining bases are the same. Preferably, the 299th base is substituted with C to T.

FIGS. 6A to 6C show the nucleotide sequences of the wild type cDNA and the nucleotide sequences of SEQ ID NO: 2. The wild-type cDNA base sequence is the same as the nucleotide sequence of SEQ ID NO: 4. Referring to FIG. 6B, it can be confirmed that the nucleotide sequence of SEQ ID NO: 2 differs from the nucleotide sequence of SEQ ID NO: 4 only in the 676th nucleotide, and all the remaining bases are the same. Preferably, the 676th base is substituted by A to T.

7 compares the polypeptide sequence of the wild type polypeptide with the polypeptide sequence of SEQ ID NO: 5. The wild-type polypeptide sequence is a polypeptide sequence encoded by the nucleotide sequence of SEQ ID NO: 3. Comparing the two sequences, it can be seen that only the 299th base in the nucleotide sequence of SEQ ID NO: 3 was replaced by C to T, so that the 100th amino acid of SEQ ID NO: 5 was replaced with isoleucine in the existing threonine. It is confirmed that all the amino acids contained in the other polypeptide sequences are the same.

Figure 8 compares the polypeptide sequence of the wild type polypeptide with the polypeptide sequence of SEQ ID NO: 6. The wild-type polypeptide sequence is a polypeptide sequence encoded by the nucleotide sequence of SEQ ID NO: 4. Comparing the two sequences, it can be seen that only the 676th base in the nucleotide sequence of SEQ ID NO: 4 was replaced by A to T, so that the polypeptide of SEQ ID NO: 6 contained only 208 amino acids in total and was terminated. This is because the nucleotide sequence encoding the existing Lysine is a termination codon as the base A is substituted with T. The other polypeptides of SEQ ID NO: 6 are identical to the polypeptides encoded by the nucleotide sequence of SEQ ID NO: 4 up to the 208th amino acid.

Figure 9 is a simplified representation of the manner in which the genetically modified tomato plants of the present invention are obtained. The X shown in Fig. 9 means mating and the X drawn with a circle means self-correction. The light blue and blue bars indicate respectively the chromatin powder and the yellow line on the chromatin powder indicates that the mutation occurred. Genetic Linkage Mapping analysis and Next Generation sequencing (NGS) were used for sequence analysis.

The genetically modified tomato plants of the present invention were obtained by crossing a mutant tomato plant with positive roots with wild tomato plants. More specifically, a hybrid F1 mother line is obtained by crossing a mutant tomato plant with a positive actininess and a S. pimpinellifolium . The F2 mapping group is constructed by self-correction of the hybrid F1 generation.

The approximate location of the mutant gene is confirmed by using the nucleotide sequence of the previously constructed mutant tomatoes and wild tomatoes as indel markers. Genomic DNA of more than 20 mutant phenotype tomato plants and more than 20 wild type phenotype tomato plants contained in the F2 mapping group are separated and DNA pool is formed and whole genome sequencing (WGS) is performed .

Wild type phenotype (Negative roots) Tomato Plants and Mutant phenotype (Positive roots) Tomatoes WSG results of the plants are analyzed to reveal mutant nucleotide sequences. In detail, the single nucleotide polymorphism (SNP) of the chromosome, which is confirmed to be mutated, needs to be considered. Finally, we reverse-link three or more plants showing mutant phenotype to verify the association between genotype and phenotype.

Hereinafter, a tomato plant including a mutant base sequence secured by the above-described method and a wild-type tomato plant are compared as an example.

{Example}

Example 1. LAZY1-mutant  Tomato plants containing the base sequence

ATGAAGTTAC TTGGTTGGAT GCATCGTAAG TTTCGACAGA ATAGCAGTGA ACCACTAAAG 60

GAATTTTCTG TAGGAAATCC TTGTACTTGT CTTACTGGGC AGCCATCACT AGATGATATA 120

CATTGCTACC CAAAGTCAAA TTTTTACAAT AAGTCTTTGA GTAAAACTCA GAGAGACAAT 180

CACTTGAGAA AATCATTTGC TGGTCTAGAG GCAGCAGCAA GGGCAGATCA TTACGATCTC 240

GAAGAAGAGT CATCTGCTGC TCTTTCTGAG TTGTTCCATG GATTCCTTGC AATTGGTATA 300

CTTGGTACTG ATCCCCTTCT TGATGATCCT TCAACACCGA CATTTTCCAT CTCTGTGGAA 360

AATATTGCTG AAAAGGATAC TGAAGTGACT GAAAATGAAC TGAAGCTGAT TAATGATGAA 420

CTCGAGAAGG TGTTGGGAGC TGAAGCTAAA GATGATACTT GTAATCTTTC TTCAGGAAGA 480

AACAGCTATG TTAGCACTGG AAGAAGCAGT CATGGTAGCA CCATTACACT AAGTGGAAAG 540

CATTGGAAA GTGCAGAGAA CAATGGAAAC GGAACAACGG TCTGTCCACT TCAGGGCTAT 600

CTATTTGGAT CAACTGTTGA GATGCAAGAG ACAACTTCTG CTTCTGCTAA GAAAGAACAT 660

AGGCCTTCGC TCGGTGAGCT ATTTCAAAAA ACTAAACTAG CAGAAGAAAA CTATGGACCT 720

AAATATCATG AGAAGCGAAC AGATAAGGAT TCTGATAAAT CTGCTGTGCA CCTCATGAAG 780

AAAATACTGA AGAAAAAGAT GCTTCATGCT TCCTCCCGAA ACTCAGTATC AGCTTCAGGG 840

GGAACTGTTG ATTCTGTTTC AGCAGAGTCA AAACCCCATA AGATCCTACA AATGTTCCAC 900

AGAAAAGTCC ATCCTGAGAG CTCAATGAAG CCTGATAAAT TCTTAAAAAA TGAGAGGGCA 960

CATGACCGTG GAGGACTATC ACTTGCACGT GATGATATCA CAATTATCCC TCACCATCGC 1020

CTCTCAAAGG ACAGCATCAA GGGTCAGCCT ATCATGCAAC AGTCCACGGT AGATGGTGAT 1080

TCAAATGAAA ACCGAGAATG CTGGATCAAA ACTGATGCAG ATTACTTTGT GCTGGAGCTC 1140

TAA 1143

Example 2. SCR-mutant  Tomato plants containing the base sequence

ATGGCCAAGG CTTTACCTGT GAGTGGTGAG AACTGCACCG GTGTGAACGT CGTCGGTGAT 60

AGTAGTGGTG AGAAGACAAG CAAGCAACTT TTCCACTGTG AAAACGGAAA AATGGTTAGG 120

AAGAGAGCGG CGTCTGACAT GGAGATCCAG ACCGGCGCCG GTGAAGAGCA TAGATATTTA 180

CGCCGGCCTG CTATGATAGG GGGGTCTCAT TCTCAGGTTG GTGATTCAAG GGTTTGTGGT 240

AATAGTAATT TTGGTCATGG TATGAACAGT AATTTGACAA CGACGATGAC GATGACGACT 300

CAGGTTTCTA ACTACTCCAC TATGCAAATG TTACCTTCTT CCACGAACTT GTGCGGTGTG 360

ACGTCAAGAG GAGGGCCTGG GATTGATACC GGATTTTCTA ATTCAACCCC AAACCTAACT 420

TACACAGACG CCATAACATC GCATCATCAG CCACAAGGTA CTCAAACCCA GAACAACAAC 480

AGTCAATCCC CATCTGTTTG TGTTTTCTCC GGTCTGCCGC TTTTTCCTCC AGATAGAAAC 540

CGCCAAAACA GTGGTTTACT ACTGCAACAA CCTCCTGCTG CTGCAGCAGC TGTTGTATCT 600

TCACCTCTTA CAACTGGAAG AATCGATTCC ATGGAGGATA GTACGTCGGC AACGGCATGG 660

ATTGACAGTA TTATATAGGA TTTAATCAAC AGCTCCGCAC AAGTATCGGT GCCCCAACTC 720

ATACAGAATG TAAGGGAGAT TATTCACCCT TGCAATCCGT ATCTCGCATC TCTTCTCGAG 780

TACAGGCTTC GCTCTCTCAC TAGTAATAAT AATGGCGGTG CTGATCAAAA TGACCCCATG 840

GAGTGCTGGA GAAGAAAAGA ATCGCTTCCG GCACAGCTCG CTGGTTTACA ACAGGCCCAA 900

AATAATGCCA ATTTATTGCA GCACAATATT TTATCCCTTC CTGATTCCTC AAATAATCAA 960

TACTTAAACT GGGATATAGC TTTGCCAAAC AGCCATAACG CTCCGGTAGC GCCTTCTCAT 1020

AACCAGCACC AGCAGCTAGG CGGCAATAAT CCTACTGCAA CAGATCTTTC ATTTGTTACA 1080

CTTTCTCCTC AAGTGCAGCA GCAGCAACAA CAACAACAAC AAGAATCTCC CCATTCTCAT 1140

TCTCAGCAAC AGGCCGCAGT AGACTTAGAT CAACAACAGA AGCAGCAGCA ATCTTCTAGT 1200

TCTCTATCTC CAACATCGGT AGCCGACAAT AGCGCCAAAA CAAAAACTTC AACCCCAGCT 1260

CCACCAGTTC CGATCAACAC GTACCGAGAA AAAAAGGAAG AAGAACGACA ACAGAAGAGG 1320

GATGAAGAAG GATTACACCT TCTGACCTTG CTATTGCAAT GTGCAGAAGC CGTATCCGCT 1380

GATAATTTAG AAGAGGCAAA TAAAATGCTA TTAGAAGTAT CCGAACTCTC CACACCGTTC 1440

GGCACATCTG CACAGCGTGT TGCAGCATAT TTCTCAGAAG CCATGTCAGC AAGGCTGTTG 1500

AATTCATGCT TAGGAATATA CGCAGCCCTA CCAATGACCA GCGTCCCCAT GCTTTACACA 1560

CAGAAAATGG CATCTGCTTT CCAGGTGTTT AACGGAATCA GCCCTTTCAT CAAATTCTCA 1620

CATTTCACAG CCAATCAAGC TATACAAGAA GCTTTCGAGA GAGAAGACCG GGTACACATA 1680

ATCGACCTTG ATATAATGCA AGGTCTTCAA TGGCCCGGTC TTTTCCACAT CTTGGCTTCC 1740

AGGCCCGGTG GACCTCCTTA CGTTCGGCTC ACGGGGCTTG GAACCTCGAT GGATGCTCTT 1800

GAAGCAACCG GCAAACGACT ATCCGATTTT GCCGAACGAT TAGGCCTTCC TTTCGAGTTT 1860

TTACCAGTTG CTGATAAAGT TGGAAACTTA GACCCTGAGA AATTGAATGT GAGCAAAAGA 1920

GAAGCTGTAG CAGTTCATTG GTTGCAACAT TCCCTTTACG ATGTTACTGG CAGCGACCCT 1980

AATACACTCT CCCTCTTACA AAGGTTGGCT CCAAAGGTGG TGACGGTGGT GGAGCAGGAT 2040

CTGAGCCACG CGGGTTCATT CCTGGGGAGG TTTGTAGAGG CAATACATTA CTACTCAGCT 2100

CTATTTGACT CACTGGGCGC GTGTTACGGG GAGGAGAGTG AAGAACGACA CGTGGTGGAG 2160

CAACAACTAT TATCAAAGGA GATACGTAAT GTACTAGCCG TAGGAGGTCC ATCAAGGAGC 2220

GGGGATGCTA AATTCAACAA CTGGAGAGAG AAGCTTCAAC AATCTGGATT TAGATCCTTG 2280

TCTCTTGCAG GTAATGCTGC TGCACAAGCC ACTCTTTTAC TCGGAATGTT CCCGTCTCAT 2340

GGATACACTT TGGTGGAGGA TAATGGAACA CTTAAGCTTG GATGGAAAGA TCTTTGCTTG 2400

TTTACTGCTT CTGCATGGAG ACCTAATTCA TTGCATGCTG CACCAGGTTC TCGTCACTTC 2460

TCTCGTCCTA ATATGGATTAE 2481

Comparative Example 1 LAZY1  Tomato plants containing wild type sequence.

ATGAAGTTAC TTGGTTGGAT GCATCGTAAG TTTCGACAGA ATAGCAGTGA ACCACTAAAG 60

GAATTTTCTG TAGGAAATCC TTGTACTTGT CTTACTGGGC AGCCATCACT AGATGATATA 120

CATTGCTACC CAAAGTCAAA TTTTTACAAT AAGTCTTTGA GTAAAACTCA GAGAGACAAT 180

CACTTGAGAA AATCATTTGC TGGTCTAGAG GCAGCAGCAA GGGCAGATCA TTACGATCTC 240

GAAGAAGAGT CATCTGCTGC TCTTTCTGAG TTGTTCCATG GATTCCTTGC AATTGGTACA 300

CTTGGTACTG ATCCCCTTCT TGATGATCCT TCAACACCGA CATTTTCCAT CTCTGTGGAA 360

AATATTGCTG AAAAGGATAC TGAAGTGACT GAAAATGAAC TGAAGCTGAT TAATGATGAA 420

CTCGAGAAGG TGTTGGGAGC TGAAGCTAAA GATGATACTT GTAATCTTTC TTCAGGAAGA 480

AACAGCTATG TTAGCACTGG AAGAAGCAGT CATGGTAGCA CCATTACACT AAGTGGAAAG 540

CATTGGAAA GTGCAGAGAA CAATGGAAAC GGAACAACGG TCTGTCCACT TCAGGGCTAT 600

CTATTTGGAT CAACTGTTGA GATGCAAGAG ACAACTTCTG CTTCTGCTAA GAAAGAACAT 660

AGGCCTTCGC TCGGTGAGCT ATTTCAAAAA ACTAAACTAG CAGAAGAAAA CTATGGACCT 720

AAATATCATG AGAAGCGAAC AGATAAGGAT TCTGATAAAT CTGCTGTGCA CCTCATGAAG 780

AAAATACTGA AGAAAAAGAT GCTTCATGCT TCCTCCCGAA ACTCAGTATC AGCTTCAGGG 840

GGAACTGTTG ATTCTGTTTC AGCAGAGTCA AAACCCCATA AGATCCTACA AATGTTCCAC 900

AGAAAAGTCC ATCCTGAGAG CTCAATGAAG CCTGATAAAT TCTTAAAAAA TGAGAGGGCA 960

CATGACCGTG GAGGACTATC ACTTGCACGT GATGATATCA CAATTATCCC TCACCATCGC 1020

CTCTCAAAGG ACAGCATCAA GGGTCAGCCT ATCATGCAAC AGTCCACGGT AGATGGTGAT 1080

TCAAATGAAA ACCGAGAATG CTGGATCAAA ACTGATGCAG ATTACTTTGT GCTGGAGCTC 1140

TAA 1143

Comparative Example 2 SCR  Tomato plants containing wild type sequence.

ATGGCCAAGG CTTTACCTGT GAGTGGTGAG AACTGCACCG GTGTGAACGT CGTCGGTGAT 60

AGTAGTGGTG AGAAGACAAG CAAGCAACTT TTCCACTGTG AAAACGGAAA AATGGTTAGG 120

AAGAGAGCGG CGTCTGACAT GGAGATCCAG ACCGGCGCCG GTGAAGAGCA TAGATATTTA 180

CGCCGGCCTG CTATGATAGG GGGGTCTCAT TCTCAGGTTG GTGATTCAAG GGTTTGTGGT 240

AATAGTAATT TTGGTCATGG TATGAACAGT AATTTGACAA CGACGATGAC GATGACGACT 300

CAGGTTTCTA ACTACTCCAC TATGCAAATG TTACCTTCTT CCACGAACTT GTGCGGTGTG 360

ACGTCAAGAG GAGGGCCTGG GATTGATACC GGATTTTCTA ATTCAACCCC AAACCTAACT 420

TACACAGACG CCATAACATC GCATCATCAG CCACAAGGTA CTCAAACCCA GAACAACAAC 480

AGTCAATCCC CATCTGTTTG TGTTTTCTCC GGTCTGCCGC TTTTTCCTCC AGATAGAAAC 540

CGCCAAAACA GTGGTTTACT ACTGCAACAA CCTCCTGCTG CTGCAGCAGC TGTTGTATCT 600

TCACCTCTTA CAACTGGAAG AATCGATTCC ATGGAGGATA GTACGTCGGC AACGGCATGG 660

ATTGACAGTA TTATAAAGGA TTTAATCAAC AGCTCCGCAC AAGTATCGGT GCCCCAACTC 720

ATACAGAATG TAAGGGAGAT TATTCACCCT TGCAATCCGT ATCTCGCATC TCTTCTCGAG 780

TACAGGCTTC GCTCTCTCAC TAGTAATAAT AATGGCGGTG CTGATCAAAA TGACCCCATG 840

GAGTGCTGGA GAAGAAAAGA ATCGCTTCCG GCACAGCTCG CTGGTTTACA ACAGGCCCAA 900

AATAATGCCA ATTTATTGCA GCACAATATT TTATCCCTTC CTGATTCCTC AAATAATCAA 960

TACTTAAACT GGGATATAGC TTTGCCAAAC AGCCATAACG CTCCGGTAGC GCCTTCTCAT 1020

AACCAGCACC AGCAGCTAGG CGGCAATAAT CCTACTGCAA CAGATCTTTC ATTTGTTACA 1080

CTTTCTCCTC AAGTGCAGCA GCAGCAACAA CAACAACAAC AAGAATCTCC CCATTCTCAT 1140

TCTCAGCAAC AGGCCGCAGT AGACTTAGAT CAACAACAGA AGCAGCAGCA ATCTTCTAGT 1200

TCTCTATCTC CAACATCGGT AGCCGACAAT AGCGCCAAAA CAAAAACTTC AACCCCAGCT 1260

CCACCAGTTC CGATCAACAC GTACCGAGAA AAAAAGGAAG AAGAACGACA ACAGAAGAGG 1320

GATGAAGAAG GATTACACCT TCTGACCTTG CTATTGCAAT GTGCAGAAGC CGTATCCGCT 1380

GATAATTTAG AAGAGGCAAA TAAAATGCTA TTAGAAGTAT CCGAACTCTC CACACCGTTC 1440

GGCACATCTG CACAGCGTGT TGCAGCATAT TTCTCAGAAG CCATGTCAGC AAGGCTGTTG 1500

AATTCATGCT TAGGAATATA CGCAGCCCTA CCAATGACCA GCGTCCCCAT GCTTTACACA 1560

CAGAAAATGG CATCTGCTTT CCAGGTGTTT AACGGAATCA GCCCTTTCAT CAAATTCTCA 1620

CATTTCACAG CCAATCAAGC TATACAAGAA GCTTTCGAGA GAGAAGACCG GGTACACATA 1680

ATCGACCTTG ATATAATGCA AGGTCTTCAA TGGCCCGGTC TTTTCCACAT CTTGGCTTCC 1740

AGGCCCGGTG GACCTCCTTA CGTTCGGCTC ACGGGGCTTG GAACCTCGAT GGATGCTCTT 1800

GAAGCAACCG GCAAACGACT ATCCGATTTT GCCGAACGAT TAGGCCTTCC TTTCGAGTTT 1860

TTACCAGTTG CTGATAAAGT TGGAAACTTA GACCCTGAGA AATTGAATGT GAGCAAAAGA 1920

GAAGCTGTAG CAGTTCATTG GTTGCAACAT TCCCTTTACG ATGTTACTGG CAGCGACCCT 1980

AATACACTCT CCCTCTTACA AAGGTTGGCT CCAAAGGTGG TGACGGTGGT GGAGCAGGAT 2040

CTGAGCCACG CGGGTTCATT CCTGGGGAGG TTTGTAGAGG CAATACATTA CTACTCAGCT 2100

CTATTTGACT CACTGGGCGC GTGTTACGGG GAGGAGAGTG AAGAACGACA CGTGGTGGAG 2160

CAACAACTAT TATCAAAGGA GATACGTAAT GTACTAGCCG TAGGAGGTCC ATCAAGGAGC 2220

GGGGATGCTA AATTCAACAA CTGGAGAGAG AAGCTTCAAC AATCTGGATT TAGATCCTTG 2280

TCTCTTGCAG GTAATGCTGC TGCACAAGCC ACTCTTTTAC TCGGAATGTT CCCGTCTCAT 2340

GGATACACTT TGGTGGAGGA TAATGGAACA CTTAAGCTTG GATGGAAAGA TCTTTGCTTG 2400

TTTACTGCTT CTGCATGGAG ACCTAATTCA TTGCATGCTG CACCAGGTTC TCGTCACTTC 2460

TCTCGTCCTA ATATGGATTAE 2481

{evaluation}

1. Actuation evaluation

Sixteen tomato plants of the plants of Examples 1, 2 and Comparative Examples 1 and 2 were thoroughly planted. As a result, all the tomato plants of Examples 1 and 2 were positive for stem roots, whereas the tomato plants of Comparative Examples 1 and 2 were all negative for stem roots.

2. Comparison of number of stem and number of peduncle

The number of stems and the number of peduncles after 60 days after 16 planting of tomato plants of Examples 1 and 2 and Comparative Examples 1 and 2 were compared as follows. The number of stems of the animal topography was 4 on average in Examples 1 and 2, and 4 on average in Comparative Examples 1 and 2. On the other hand, the number of flower buds was about 5 in the case of Example 1, and about 5 in the case of Example 2, and was about 5 in the Comparative Example 1 and 5 in the Comparative Example 2.

It can be predicted that the tomato yields of tomato plants per plant are increased as the number of stems and the number of stalks are increased, so that the approximate tomato yields of Examples 1 and 2 and Comparative Examples 1 and 2 will be similar.

3. Overall evaluation

Compared to the tomato plants of Examples 1 and 2, the yield of tomato was similar but the stem of the stem was positive. Therefore, by cultivating the tomato plant with different cultivation height, the yield of tomato per unit area Was significantly increased.

The tomato plants of Examples 1 and 2 were also found to have the effect of reducing the labor required for cultivation compared to the existing tomato plants when cultivating tomato plants.

<110> Wonkwang University Center for Industry-Academy Cooperation <120> Genetically modified tomato plants with a novel growth pattern &Lt; 130 > KL-P-01862 <160> 6 <170> KoPatentin 3.0 <210> 1 <211> 1143 <212> DNA <213> Lycopersicon esculentum <400> 1 atgaagttac ttggttggat gcatcgtaag tttcgacaga atagcagtga accactaaag 60 gaattttctg taggaaatcc ttgtacttgt cttactgggc agccatcact agatgatata 120 cattgctacc caaagtcaaa tttttacaat aagtctttga gtaaaactca gagagacaat 180 cacttgagaa aatcatttgc tggtctagag gcagcagcaa gggcagatca ttacgatctc 240 gaagaagagt catctgctgc tctttctgag ttgttccatg gattccttgc aattggtata 300 cttggtactg atccccttct tgatgatcct tcaacaccga cattttccat ctctgtggaa 360 aatattgctg aaaaggatac tgaagtgact gaaaatgaac tgaagctgat taatgatgaa 420 ctcgagaagg tgttgggagc tgaagctaaa gatgatactt gtaatctttc ttcaggaaga 480 aagactatg ttagcactgg aagaagcagt catggtagca ccattacact aagtggaaag 540 caattggaaa gtgcagagaa caatggaaac ggaacaacgg tctgtccact tcagggctat 600 ctatttggat caactgttga gatgcaagag acaacttctg cttctgctaa gaaagaacat 660 aggccttcgc tcggtgagct atttcaaaaa actaaactag cagaagaaaa ctatggacct 720 cctcatgaag 780 aaaatactga agaaaaagat gcttcatgct tcctcccgaa actcagtatc agcttcaggg 840 ggaactgttg attctgtttc agcagagtca aaaccccata agatcctaca aatgttccac 900 agaaaagtcc atcctgagag ctcaatgaag cctgataaat tcttaaaaaa tgagagggca 960 catgaccgtg gaggactatc acttgcacgt gatgatatca caattatccc tcaccatcgc 1020 ctctcaaagg acagcatcaa gggtcagcct atcatgcaac agtccacggt agatggtgat 1080 tcaaatgaaa accgagaatg ctggatcaaa actgatgcag attactttgt gctggagctc 1140 taa 1143 <210> 2 <211> 2481 <212> DNA <213> Lycopersicon esculentum <400> 2 atggccaagg ctttacctgt gagtggtgag aactgcaccg gtgtgaacgt cgtcggtgat 60 agtagtggtg agaagacaag caagcaactt ttccactgtg aaaacggaaa aatggttagg 120 aagagagcgg cgtctgacat ggagatccag accggcgccg gtgaagagca tagatattta 180 cgccggcctg ctatgatagg ggggtctcat tctcaggttg gtgattcaag ggtttgtggt 240 aatagtaatt ttggtcatgg tatgaacagt aatttgacaa cgacgatgac gatgacgact 300 caggtttcta actactccac tatgcaaatg ttaccttctt ccacgaactt gtgcggtgtg 360 acgtcaagag gagggcctgg gattgatacc ggattttcta attcaacccc aaacctaact 420 tacacagacg ccataacatc gcatcatcag ccacaaggta ctcaaaccca gaacaacaac 480 agtcaatccc catctgtttg tgttttctcc ggtctgccgc tttttcctcc agatagaaac 540 cgccaaaaca gtggtttact actgcaacaa cctcctgctg ctgcagcagc tgttgtatct 600 aacggcatgg attgacagta ttatatagga tttaatcaac agctccgcac aagtatcggt gccccaactc 720 atacagaatg taagggagat tattcaccct tgcaatccgt atctcgcatc tcttctcgag 780 tacaggcttc gctctctcac tagtaataat aatggcggtg ctgatcaaaa tgaccccatg 840 gagtgctgga gaagaaaaga atcgcttccg gcacagctcg ctggtttaca acaggcccaa 900 aataatgcca atttattgca gcacaatatt ttatcccttc ctgattcctc aaataatcaa 960 tacttaaact gggatatagc tttgccaaac agccataacg ctccggtagc gccttctcat 1020 aaccagcacc agcagctagg cggcaataat cctactgcaa cagatctttc atttgttaca 1080 ctttctcctc aagtgcagca gcagcaacaa caacaacaac aagaatctcc ccattctcat 1140 tctcagcaac aggccgcagt agacttagat caacaacaga agcagcagca atcttctagt 1200 tctctatctc caacatcggt agccgacaat agcgccaaaa caaaaacttc aaccccagct 1260 ccaccagttc cgatcaacac gtaccgagaa aaaaaggaag aagaacgaca acagaagagg 1320 gatgaagaag gattacacct tctgaccttg ctattgcaat gtgcagaagc cgtatccgct 1380 gataatttag aagaggcaaa taaaatgcta ttagaagtat ccgaactctc cacaccgttc 1440 ggcacatctg cacagcgtgt tgcagcatat ttctcagaag ccatgtcagc aaggctgttg 1500 aattcatgct taggaatata cgcagcccta ccaatgacca gcgtccccat gctttacaca 1560 cagaaaatgg catctgcttt ccaggtgttt aacggaatca gccctttcat caaattctca 1620 catttcacag ccaatcaagc tatacaagaa gctttcgaga gagaagaccg ggtacacata 1680 atcgaccttg atataatgca aggtcttcaa tggcccggtc ttttccacat cttggcttcc 1740 aggcccggtg gacctcctta cgttcggctc acggggcttg gaacctcgat ggatgctctt 1800 gaagcaaccg gcaaacgact atccgatttt gccgaacgat taggccttcc tttcgagttt 1860 ttaccagttg ctgataaagt tggaaactta gaccctgaga aattgaatgt gagcaaaaga 1920 gaagctgtag cagttcattg gttgcaacat tccctttacg atgttactgg cagcgaccct 1980 aatacactct ccctcttaca aaggttggct ccaaaggtgg tgacggtggt ggagcaggat 2040 ctgagccacg cgggttcatt cctggggagg tttgtagagg caatacatta ctactcagct 2100 ctatttgact cactgggcgc gtgttacggg gaggagagtg aagaacgaca cgtggtggag 2160 caacaactat tatcaaagga gatacgtaat gtactagccg taggaggtcc atcaaggagc 2220 ggggatgcta aattcaacaa ctggagagag aagcttcaac aatctggatt tagatccttg 2280 tctcttgcag gtaatgctgc tgcacaagcc actcttttac tcggaatgtt cccgtctcat 2340 ggatacactt tggtggagga taatggaaca cttaagcttg gatggaaaga tctttgcttg 2400 tttactgctt ctgcatggag acctaattca ttgcatgctg caccaggttc tcgtcacttc 2460 tctcgtccta atatggatta a 2481 <210> 3 <211> 1143 <212> DNA <213> Lycopersicon esculentum <400> 3 atgaagttac ttggttggat gcatcgtaag tttcgacaga atagcagtga accactaaag 60 gaattttctg taggaaatcc ttgtacttgt cttactgggc agccatcact agatgatata 120 cattgctacc caaagtcaaa tttttacaat aagtctttga gtaaaactca gagagacaat 180 cacttgagaa aatcatttgc tggtctagag gcagcagcaa gggcagatca ttacgatctc 240 gaagaagagt catctgctgc tctttctgag ttgttccatg gattccttgc aattggtaca 300 cttggtactg atccccttct tgatgatcct tcaacaccga cattttccat ctctgtggaa 360 aatattgctg aaaaggatac tgaagtgact gaaaatgaac tgaagctgat taatgatgaa 420 ctcgagaagg tgttgggagc tgaagctaaa gatgatactt gtaatctttc ttcaggaaga 480 aagactatg ttagcactgg aagaagcagt catggtagca ccattacact aagtggaaag 540 caattggaaa gtgcagagaa caatggaaac ggaacaacgg tctgtccact tcagggctat 600 ctatttggat caactgttga gatgcaagag acaacttctg cttctgctaa gaaagaacat 660 aggccttcgc tcggtgagct atttcaaaaa actaaactag cagaagaaaa ctatggacct 720 cctcatgaag 780 aaaatactga agaaaaagat gcttcatgct tcctcccgaa actcagtatc agcttcaggg 840 ggaactgttg attctgtttc agcagagtca aaaccccata agatcctaca aatgttccac 900 agaaaagtcc atcctgagag ctcaatgaag cctgataaat tcttaaaaaa tgagagggca 960 catgaccgtg gaggactatc acttgcacgt gatgatatca caattatccc tcaccatcgc 1020 ctctcaaagg acagcatcaa gggtcagcct atcatgcaac agtccacggt agatggtgat 1080 tcaaatgaaa accgagaatg ctggatcaaa actgatgcag attactttgt gctggagctc 1140 taa 1143 <210> 4 <211> 2481 <212> DNA <213> Lycopersicon esculentum <400> 4 atggccaagg ctttacctgt gagtggtgag aactgcaccg gtgtgaacgt cgtcggtgat 60 agtagtggtg agaagacaag caagcaactt ttccactgtg aaaacggaaa aatggttagg 120 aagagagcgg cgtctgacat ggagatccag accggcgccg gtgaagagca tagatattta 180 cgccggcctg ctatgatagg ggggtctcat tctcaggttg gtgattcaag ggtttgtggt 240 aatagtaatt ttggtcatgg tatgaacagt aatttgacaa cgacgatgac gatgacgact 300 caggtttcta actactccac tatgcaaatg ttaccttctt ccacgaactt gtgcggtgtg 360 acgtcaagag gagggcctgg gattgatacc ggattttcta attcaacccc aaacctaact 420 tacacagacg ccataacatc gcatcatcag ccacaaggta ctcaaaccca gaacaacaac 480 agtcaatccc catctgtttg tgttttctcc ggtctgccgc tttttcctcc agatagaaac 540 cgccaaaaca gtggtttact actgcaacaa cctcctgctg ctgcagcagc tgttgtatct 600 aacggcatgg attgacagta ttataaagga tttaatcaac agctccgcac aagtatcggt gccccaactc 720 atacagaatg taagggagat tattcaccct tgcaatccgt atctcgcatc tcttctcgag 780 tacaggcttc gctctctcac tagtaataat aatggcggtg ctgatcaaaa tgaccccatg 840 gagtgctgga gaagaaaaga atcgcttccg gcacagctcg ctggtttaca acaggcccaa 900 aataatgcca atttattgca gcacaatatt ttatcccttc ctgattcctc aaataatcaa 960 tacttaaact gggatatagc tttgccaaac agccataacg ctccggtagc gccttctcat 1020 aaccagcacc agcagctagg cggcaataat cctactgcaa cagatctttc atttgttaca 1080 ctttctcctc aagtgcagca gcagcaacaa caacaacaac aagaatctcc ccattctcat 1140 tctcagcaac aggccgcagt agacttagat caacaacaga agcagcagca atcttctagt 1200 tctctatctc caacatcggt agccgacaat agcgccaaaa caaaaacttc aaccccagct 1260 ccaccagttc cgatcaacac gtaccgagaa aaaaaggaag aagaacgaca acagaagagg 1320 gatgaagaag gattacacct tctgaccttg ctattgcaat gtgcagaagc cgtatccgct 1380 gataatttag aagaggcaaa taaaatgcta ttagaagtat ccgaactctc cacaccgttc 1440 ggcacatctg cacagcgtgt tgcagcatat ttctcagaag ccatgtcagc aaggctgttg 1500 aattcatgct taggaatata cgcagcccta ccaatgacca gcgtccccat gctttacaca 1560 cagaaaatgg catctgcttt ccaggtgttt aacggaatca gccctttcat caaattctca 1620 catttcacag ccaatcaagc tatacaagaa gctttcgaga gagaagaccg ggtacacata 1680 atcgaccttg atataatgca aggtcttcaa tggcccggtc ttttccacat cttggcttcc 1740 aggcccggtg gacctcctta cgttcggctc acggggcttg gaacctcgat ggatgctctt 1800 gaagcaaccg gcaaacgact atccgatttt gccgaacgat taggccttcc tttcgagttt 1860 ttaccagttg ctgataaagt tggaaactta gaccctgaga aattgaatgt gagcaaaaga 1920 gaagctgtag cagttcattg gttgcaacat tccctttacg atgttactgg cagcgaccct 1980 aatacactct ccctcttaca aaggttggct ccaaaggtgg tgacggtggt ggagcaggat 2040 ctgagccacg cgggttcatt cctggggagg tttgtagagg caatacatta ctactcagct 2100 ctatttgact cactgggcgc gtgttacggg gaggagagtg aagaacgaca cgtggtggag 2160 caacaactat tatcaaagga gatacgtaat gtactagccg taggaggtcc atcaaggagc 2220 ggggatgcta aattcaacaa ctggagagag aagcttcaac aatctggatt tagatccttg 2280 tctcttgcag gtaatgctgc tgcacaagcc actcttttac tcggaatgtt cccgtctcat 2340 ggatacactt tggtggagga taatggaaca cttaagcttg gatggaaaga tctttgcttg 2400 tttactgctt ctgcatggag acctaattca ttgcatgctg caccaggttc tcgtcacttc 2460 tctcgtccta atatggatta a 2481 <210> 5 <211> 380 <212> PRT <213> Lycopersicon esculentum <400> 5 Met Lys Leu Leu Gly Trp Met His Arg Lys Phe Arg Gln Asn Ser Ser   1 5 10 15 Glu Pro Leu Lys Glu Phe Ser Val Gly Asn Pro Cys Thr Cys Leu Thr              20 25 30 Gly Gln Pro Ser Leu Asp Asp Ile His Cys Tyr Pro Lys Ser Asn Phe          35 40 45 Tyr Asn Lys Ser Leu Ser Lys Thr Gln Arg Asp Asn His Leu Arg Lys      50 55 60 Ser Phe Ala Gly Leu Glu Ala Ala Ala Arg Ala Asp His Tyr Asp Leu  65 70 75 80 Glu Glu Ser Ser Ala Ala Leu Ser Glu Leu Phe His Gly Phe Leu                  85 90 95 Ala Ile Gly Ile Leu Gly Thr Asp Pro Leu Leu Asp Asp Pro Ser Thr             100 105 110 Pro Thr Phe Ser Ile Ser Val Glu Asn Ile Ala Glu Lys Asp Thr Glu         115 120 125 Val Thr Glu Asn Glu Leu Lys Leu Ile Asn Asp Glu Leu Glu Lys Val     130 135 140 Leu Gly Ala Glu Ala Lys Asp Asp Thr Cys Asn Leu Ser Ser Gly Arg 145 150 155 160 Asn Ser Tyr Val Ser Thr Gly Arg Ser Ser His Gly Ser Thr Ile Thr                 165 170 175 Leu Ser Gly Lys Gln Leu Glu Ser Ala Glu Asn Asn Gly Asn Gly Thr             180 185 190 Thr Val Cys Pro Leu Gln Gly Tyr Leu Phe Gly Ser Thr Val Glu Met         195 200 205 Gln Glu Thr Thr Ser Ala Ser Ala Lys Lys Glu His Arg Pro Ser Leu     210 215 220 Gly Glu Leu Phe Gln Lys Thr Lys Leu Ala Glu Glu Asn Tyr Gly Pro 225 230 235 240 Lys Tyr His Glu Lys Arg Thr Asp Lys Asp Ser Asp Lys Ser Ala Val                 245 250 255 His Leu Met Lys Lys Ile Leu Lys Lys Lys Met Leu His Ala Ser Ser             260 265 270 Arg Asn Ser Val Ser Ala Ser Gly Gly Thr Val Asp Ser Val Ser Ala         275 280 285 Glu Ser Lys Pro His Lys Ile Leu Gln Met Phe His Arg Lys Val His     290 295 300 Pro Glu Ser Ser Lys Pro Asp Lys Phe Leu Lys Asn Glu Arg Ala 305 310 315 320 His Asp Arg Gly Gly Leu Ser Leu Ala Arg Asp Asp Ile Thr Ile Ile                 325 330 335 Pro His His Arg Leu Ser Lys Asp Ser Ile Lys Gly Gln Pro Ile Met             340 345 350 Gln Gln Ser Thr Val Asp Gly Asp Ser Asn Glu Asn Arg Glu Cys Trp         355 360 365 Ile Lys Thr Asp Ala Asp Tyr Phe Val Leu Glu Leu     370 375 380 <210> 6 <211> 225 <212> PRT <213> Lycopersicon esculentum <400> 6 Met Ala Lys Ala Leu Pro Val Ser Gly Glu Asn Cys Thr Gly Val Asn   1 5 10 15 Val Val Gly Asp Ser Ser Gly Glu Lys Thr Ser Lys Gln Leu Phe His              20 25 30 Cys Glu Asn Gly Lys Met Val Arg Lys Arg Ala Ala Ser Asp Met Glu          35 40 45 Ile Gln Thr Gly Ala Gly Glu Glu His Arg Tyr Leu Arg Arg Pro Ala      50 55 60 Met Ile Gly Gly Ser His Ser Gln Val Gly Asp Ser Arg Val Cys Gly  65 70 75 80 Asn Ser Asn Phe Gly His Gly Met Asn Ser Asn Leu Thr Thr Thr Met                  85 90 95 Thr Met Thr Thr Gln Val Ser Asn Tyr Ser Thr Met Gln Met Leu Pro             100 105 110 Ser Ser Thr Asn Leu Cys Gly Val Thr Ser Arg Gly Gly Pro Gly Ile         115 120 125 Asp Thr Gly Phe Ser Asn Ser Thr Pro Asn Leu Thr Tyr Thr Asp Ala     130 135 140 Ile Thr Ser His His Gln Pro Gln Gly Thr Gln Thr Gln Asn Asn Asn 145 150 155 160 Ser Gln Ser Ser Val Cys Val Phe Ser Gly Leu Pro Leu Phe Pro                 165 170 175 Pro Asp Arg Asn Arg Gln Asn Ser Gly Leu Leu Leu Gln Gln Pro Pro             180 185 190 Ala Ala Ala Ala Ala Val Ser Ser Ser Le Le Thr Thr Gly Arg Ile         195 200 205 Asp Ser Met Glu Asp Ser Thr Ser Ala Thr Ala Trp Ile Asp Ser Ile     210 215 220 Ile 225

Claims (9)

For genetically modified tomato plants,
Wherein the genetically modified tomato plant comprises the nucleotide sequence of SEQ ID NO: 1.
For genetically modified tomato plants,
Wherein the genetically modified tomato plant is characterized in that the 299th base in the nucleotide sequence of SEQ ID NO: 3 is substituted by C to T.
For genetically modified tomato plants,
Wherein the genetically modified tomato plant comprises a nucleotide sequence encoding a polypeptide of SEQ ID NO: 5.
For genetically modified tomato plants,
Wherein the genetically modified tomato plant comprises the nucleotide sequence of SEQ ID NO: 2.
For genetically modified tomato plants,
Wherein the genetically modified tomato plant is characterized in that the 676th base in the nucleotide sequence of SEQ ID NO: 4 is substituted by A to T.
For genetically modified tomato plants,
Wherein said genetically modified tomato plant comprises a nucleotide sequence encoding a polypeptide of SEQ ID NO: 6.
5. The method according to any one of claims 1 to 4,
Wherein the genetically modified tomato plant is a genetically modified tomato plant.
5. The method according to any one of claims 1 to 4,
Wherein the genetically modified tomato plant is an inbreeding hybrid.
5. The method according to any one of claims 1 to 4,
Wherein the genetically modified tomato plant is a hybrid.

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