KR102010726B1 - A Novel mutant tomato plants with modified determinate shoot architecture - Google Patents

Abstract

The present invention relates to a genetically modified finite growth tomato plant, wherein the genetically modified finite tomato plant comprises a mutant self-arrangement gene, wherein the mutant self-arrangement gene is SEQ ID NO: 1 to SEQ ID NO: : It is characterized by including any one or more of nucleotide sequences of three.

Description

A Novel mutant tomato plants with modified determinate shoot architecture with improved finite growth stem structure

The present invention relates to a novel mutant tomato plant with improved finite growth stem structure. More preferably, it relates to a finite growth type tomato plant having high probability.

Tomato is one of the most consumed vegetables around the world, and is often considered a healthy vegetable, and interest in tomatoes continues to increase in Korea. As interest in tomatoes has increased, the cultivation environment has also diversified. Representative tomato cultivation environments include field cultivation and facility cultivation.

Facility cultivation is a cultivation method that utilizes facilities such as a greenhouse, and has the advantage of maintaining a constant cultivation environment compared to field cultivation, but has a disadvantage in that the number of flowers per plant is small. Typically, determinate (D) type tomato varieties are preferred for field cultivation, and indeterminate (ID) type tomato varieties are preferred for facility cultivation.

After the self-pruning (sp ) mutation of finite-growing tomatoes was discovered in the last century, several breeders tried to control the growth and flowering patterns by using the mutation as a breeding material. Furthermore, through the study of sp mutant, it was revealed that the protein encoded by the sp gene is Antiflorigen , one of the flowering control hormones. It is also well known that mutations in tomato play an important role in determining stem growth and vegetative growth cycles in tomatoes.

Finite growth tomato varieties are known to be effective in reducing labor. In addition, the existing hybrid strength studies reveal that it is possible to secure a large number of finite growth tomato varieties by controlling the stem growth cycle. Specifically, as a method of securing majority probability, a method of increasing the production of tomato plants by increasing the number of livestock topography (sympodial) stems is commonly used.

However, the existing finite growth tomato varieties have not been successful in diversifying the stem growth pattern using only a single mutation, which means that the diversity of the cultivation period suitable for each cultivation environment cannot be guaranteed. In addition, when cultivating finite-growth tomatoes in facilities such as greenhouses, it was also a major problem that the economy was insufficient compared to labor reduction.

US registered patent US 9,157,093 B2

In order to solve the above problems, an object of the present invention is to provide a mutant finite growth type tomato plant having a variety of stem growth patterns and cultivation periods, and securing a large number of probabilities.

In order to achieve the above object, the present invention discloses a genetically modified finite growth tomato plant.

The genetically modified finite-growth tomato plant of the present invention includes a mutant self-cutting gene, and the mutant self-cutting gene may include the nucleotide sequence of SEQ ID NO: 1 (sp-5732).

The genetically modified finite-growth tomato plant of the present invention includes a mutant magnetic-arthropathy gene, and the mutant magnetic-arthroscopic gene may be substituted with A at the 382th base of SEQ ID NO: 4 (SP).

The genetically modified finite-growth tomato plant of the present invention includes a mutant self-cutting gene, and the mutant self-cutting gene may include a nucleotide sequence of SEQ ID NO: 2 (sp-2857).

The genetically modified finite-growth tomato plant of the present invention includes a mutant self-cutting gene, and the mutant self-cutting gene may include the nucleotide sequence of SEQ ID NO: 3 (sp-2798).

The genetically modified finite-growth tomato plant of the present invention includes a mutant self-cutting gene, and the mutant self-cutting gene may encode the polypeptide of SEQ ID NO: 6.

The genetically modified finite-growth tomato plant of the present invention includes a mutant self-cutting gene, and the mutant self-cutting gene may encode the polypeptide of SEQ ID NO: 7.

The genetically modified finite growth tomato plant of the present invention is purebred. The genetically modified finite growth tomato plant of the present invention is a hybrid. This genetically modified finite growth tomato plant is an inbred species.

The finite growth tomato varieties modified with the self-cutting gene of the present invention as described above provide an effect of reducing labor and increasing the amount of tomato production at the same time.

In addition, as a finite growth type tomato cultivar in which the self-cutting gene of the present invention is deleted, a tomato cultivar having a different stem growth pattern from the existing tomato cultivar and increasing the number of flowers provides a tomato cultivar with high probability.

In addition, as a finite-growth tomato variety in which some of the base sequences of the self-cutting gene of the present invention are substituted, a tomato variety having a different stem growth pattern from the existing tomato variety and an increase in the number of flowers provides a tomato variety that has secured multiplexability.

1 shows the result of electrophoresis for the mutation of the magnetic arthrodesis gene disclosed in the present invention.
Figure 2 is a conceptual diagram showing the gene structure of the magnetic arthropathy mutations newly disclosed in the present invention.
3 is a conceptual diagram classified with respect to the stem growth form of a tomato plant.
FIG. 4 is a conceptual diagram showing the number of leaves of a sympodial stem according to a mutant type.
5 is a graph showing the total number of inflorescences of magnetic arthropod gene mutations.
Figure 6 is a comparison of the polypeptide sequences between SP homologs including magnetic arthrodesis gene mutations.
7A to 7F are comparisons of the nucleotide sequences of the novel deletion mutants disclosed in the present invention.
8A to 8B are a comparison of a gene coding sequence (CDS) between novel substitution and deletion mutations disclosed in the present invention.
9 is a comparison of the sequences of polypeptides expressed by novel mutations disclosed in the present invention.

The invention disclosed in the present specification relates to a mutation of a finite growth type eggplant family, preferably a finite growth type tomato plant ( Solanum lycopersicum ) of the self-pruning gene (self-pruning gene, sp gene). The finite growth type tomato plant containing the mutation of the novel self-cutting gene appears to have increased production, preferably the production of tomato, compared to the existing finite growth type tomato plant.

The self-cutting gene is a gene that regulates the stem growth cycle of tomato plants, and mutations in the self-cutting gene appear as a change in the stem growth pattern of the tomato plant. The self-cutting gene encodes anti-flowering hormone (Antiflorigen), and anti-flowering hormone is known as a flowering inhibitor. The specific nucleotide sequence of SP Wild-Type as an auto-articular gene without mutations is the same as the nucleotide sequence ( SP ) of SEQ ID NO: 4.

Infinite growth type (ID) is a growth type in which the generation and growth of sympodial stems are continuously and repeatedly carried out, and is advantageous in terms of high probability, but has a disadvantage in that it requires considerable labor in sowing and harvesting. Tomato plants containing the nucleotide sequence of SEQ ID NO: 4 are generally grown as endless stems.

The polypeptide encoded by the nucleotide sequence of SEQ ID NO: 4 is an SP wild-type polypeptide and is the same as SEQ ID NO: 8.

The finite growth (D) type refers to a form in which sympodial does not occur continuously and its growth mode is not repeated. The nucleotide sequence, which is well known as a mutant magnetic arthropod gene, is the same as the nucleotide sequence ( sp classic ) of SEQ ID NO: 5. The nucleotide sequence ( sp classic ) is the same as the nucleotide sequence in which the 228th base of SEQ ID NO: 4 is substituted and mutated from C to T. Due to the substitution of the nucleotide sequence, the tomato plant becomes a finite growth type.

The finite growth type tomato plant shows the effect of reducing labor in sowing and harvesting, but it is disadvantageous in terms of economy because the tomato production per plant is less than that of the infinite growth type. In particular, when cultivating facilities such as greenhouses, the number of flowers per plant is absolutely insufficient, which is considered to be insufficient in economics.

In particular, the existing finite growth tomato uses only a single mutation factor ( sp classic ) to ensure the diversity of the tomato plant cultivation period, and as described above, there is still a problem that it is not easy to secure economical efficiency during facility cultivation Remained.

The new autophagy gene mutations disclosed in the present invention are different from the existing mutation factors and express various finite growth stem structures, and are genes that play a key role in the aspect of securing the multiplicity of finite growth tomato plants. It is a circle. Specifically, it includes one substitution mutation and two deletion mutations.

The process of securing the novel mutation is as follows.

First, 210 finite growth-type lines and 26 semi-determinate (SD) lines are secured to collect the mutant core group. It is examined whether there is a mutation in the SP gene (SEQ ID NO: 4) from the obtained line.

A specific method to check whether a mutation has occurred in a gene is as follows. First, the well-known sp mutation ( sp classic ) is isolated and excluded by genotyping. In order to investigate the genotype of the lines showing the SP genotype, a gene expression test (RT-PCR) is performed. Also, check the nucleotide sequence and protein of the gene coding part (CDS).

As a result, four mutant lines in which one base was substituted on the SP gene were obtained, and all four lines were confirmed to be the same substitution mutation (SEQ ID NO: 1, sp-5732). In addition, 6 lines in which deletion mutations occurred in the SP promoter region were secured, and it was confirmed that all of the 6 lines were the same deletion mutations (SEQ ID NO: 2, sp-2857). In addition, 6 lines in which deletion mutations occurred in the first intron site were secured, and it was confirmed that all of the 6 lines were the same deletion mutations (SEQ ID NO: 3, sp-2798).

In order to make the genome background of the newly obtained mutant core group the same, the M82 tomato varieties were backcrossed more than 6 times. Also cv. In the background of M82, the stem growth pattern between sp classic and the novel mutant was compared and analyzed. Table 1 shows the novel mutations of the magnetic arthrodesis gene. CC lines # in Table 1 refers to the number of specific plants in which mutations were found.

Hereinafter, more preferred embodiments of the present invention will be described in detail with reference to the drawings. However, embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below. Embodiments of the present invention are provided to more fully describe the present invention to those with average knowledge in the art, and therefore, the shapes and sizes of elements constituting the drawings will be exaggerated for a more clear explanation. I can.

According to an embodiment of the present invention, the genetically modified finite growth tomato plant contains a novel mutant magnetic arthroscopy gene, and the mutant magnetic articulation gene includes the nucleotide sequence of SEQ ID NO: 1 ( sp-5732 ). It is characterized by that. SEQ ID NO: 1 corresponds to the coding portion (CDS) of the mutant magnetic arthroscopy gene. The nucleotide sequence of SEQ ID NO: 1 encodes the polypeptide of SEQ ID NO: 6. The polypeptide of SEQ ID NO: 6 is amino acid sp-5732. According to another embodiment of the present invention, the genetically modified finite growth tomato plant contains a novel mutant magnetic arthroscopy gene, and the mutant magnetic articulation gene includes a nucleotide sequence ( sp-2857 ) of SEQ ID NO: 2 Characterized in that. The nucleotide sequence of SEQ ID NO: 2 encodes the polypeptide of SEQ ID NO: 7. The polypeptide of SEQ ID NO: 7 is amino acid sp-2857. SEQ ID NO: 2 corresponds to the coding portion (CDS) of the mutant autophagy gene. According to another embodiment of the present invention, the genetically modified finite growth tomato plant contains a novel mutant magnetic arthropod gene, and the mutant magnetic arthropathy gene has a base sequence ( sp-2798 ) of SEQ ID NO: 3 It characterized in that it includes.

According to an embodiment of the present invention, the genetically modified finite growth tomato plant includes a mutant magnetic arthropod gene, and the mutant magnetic articulation gene encodes the polypeptide of SEQ ID NO: 6. According to an embodiment of the present invention, the genetically modified finite growth tomato plant includes a mutant magnetic arthropod gene, and the mutant magnetic articulation gene encodes the polypeptide of SEQ ID NO: 7.

In addition, the nucleotide sequence ( SP ) of SEQ ID NO: 4 encodes the polypeptide of SEQ ID NO: 8. The nucleotide sequence represented by SEQ ID NO: 4 is the SP wild-type nucleotide sequence. The polypeptide of SEQ ID NO: 8 is an SP wild-type amino acid. The nucleotide sequence (SP ) of SEQ ID NO: 4 is the coding portion (CDS) of the magnetic arthroscopy gene. The nucleotide sequence ( sp-classic ) of SEQ ID NO: 5 encodes the polypeptide of SEQ ID NO: 9. The nucleotide sequence of SEQ ID NO: 5 is an sp-classic nucleotide sequence. The polypeptide of SEQ ID NO:9 is an sp-classic amino acid. The nucleotide sequence (sp-classic ) of SEQ ID NO: 5 is the coding portion (CDS) of the magnetic arthropod gene.

1 shows the result of electrophoresis for the mutation of the magnetic arthrodesis gene disclosed in the present invention. Referring to FIG. 1, new mutations ( sp-2857 and sp-5732 ) of the magnetic arthroscopy gene can be identified primarily through electrophoresis. Compared with the SP (SEQ ID NO: 4) marker and the existing mutant sp-classic (SEQ ID NO: 5) marker, as a result, both sp-2857 and sp-5732 form different bands from SP and sp-classic. Can be confirmed. In particular, sp-5732 has a finite growth type, but a band is formed at a position similar to that of the infinitely growing SP.

Figure 2 is a conceptual diagram showing the gene structure of the magnetic arthropathy mutations newly disclosed in the present invention. sp-2798 and sp-2857 represent newly developed deletion mutations. In the drawing, this is a part indicated by a dotted line. It can be seen that the deletion of sp-2857 occurred at the first intron site, and the deletion of sp-2798 occurred at the promoter part.

The yellow box in FIG. 2 is EXON, and the white box represents the untranslated area. The white box drawn with a dotted line indicates the deleted portion of the untranslated area. A new substitutional mutation, sp-5732 , can be confirmed that substitution occurred in an axon different from that of the existing finite growth type mutation ( sp-classic ). Additionally, the blue line represents the miss-spliced RNA.

3 is a conceptual diagram classified with respect to the stem growth form of a tomato plant. Bars in black and gray indicate stems that occur continuously. The black and gray ellipses mean leaves that occur in the stem, and the numbers on the leaves indicate the number of leaves that have occurred. Arrows indicate lateral buds, and green bars indicate flower stalks. Green, yellow, and red circles schematically represent the maturity of the fruit, and yellow stars represent flowers.

Tomato plants containing the nucleotide sequence (SP) of SEQ ID NO: 4 generally express an infinite growth (ID) type stem. The first stem produces 8 to 9 leaves, and the growing point is terminated by the development of flower stalks and flowers. Subsequently, the stem of sympodial develops three leaves, and the flower stalk and flowers are generated again, and the growing point is terminated. In the case of an infinitely growing tomato plant, the generation, growth, and termination of the sympodial stem are continuously and repeatedly performed. Economical efficiency is excellent because continuous livestock topography stems and tomatoes are produced in a single plant.

On the other hand, tomato plants containing the nucleotide sequence (sp-classic ) of SEQ ID NO: 5 generally express finite growth (D) type stems. After the first stem growth point produces 7 to 9 leaves, flower stalks and flowers occur, and the growth point is terminated. However, unlike infinity-growing tomato plants, the number of leaves gradually decreases from 3 to 0 in the later-occurring sympodial stems, and finally the generation of sympodial stems ends. Since the stem growth does not occur more than a certain level, it has the advantage of easy sowing and harvesting of tomatoes.

Semi-definite growth (SD) type tomato plants show a delayed pattern in the end of occurrence of sympodial stems compared to finite growth type tomato plants. Therefore, it has the characteristic of showing a stem structure with an increased number of flower stalks. In general, as a method for improving the harvestability of finite-growth tomato plants, a method of delaying the end of the occurrence of sympodial stems, like the semi-finite-growth tomato plants, is preferred.

FIG. 4 is a conceptual diagram showing the number of leaves of a sympodial stem according to a mutant type. Specifically, the number of sympodial stems and the number of leaves of the existing finite growth mutant ( sp-classic ) tomato plant and the novel mutant tomato plant disclosed in the present invention are compared. The black and gray areas in the graph indicate the number of leaves formed per sympodial stem, respectively. The number of leaves indirectly indicates the flowering time of each sympodial stem.

sp-2798 and sp- 2857 show a similar pattern of sympodial stem development to existing finite growth mutations. However, it can be seen that the number of leaves is increased compared to the existing finite growth mutation, and it can be inferred that flowering is delayed. In particular, it can be seen that sp-5732 delayed the termination of additional development of sympodial stems, and thus exhibited stem growth patterns similar to semi-finite growth tomato plants. This means that the tomato plant of sp-5732 is advantageous in securing high probability compared to the existing finite growth mutant tomato plant.

5 is a graph showing the total number of inflorescences of magnetic arthropod gene mutations. It can be inferred that as the number of flower stalks increased, the production of tomatoes per unit plant increased. Specifically, it is a graph comparing the number of stalks of mutant tomato plants grown for 3 months after transplanting. It can be seen that all of the tomato plants including sp-2798 , sp-2857 and sp-5732 increased the number of flower stalks compared to the existing finite growth mutant. In particular, it can be seen that the number of flower stalks in the tomato plant including sp-5732 increased significantly.

Figure 6 is a comparison of the polypeptide sequences between SP homologs including magnetic arthrodesis gene mutations. Specifically, SP homologs refer to several species in which the SP sequence of a common ancestor is inherited. Terminal Flower 1 (TFL1) of Arabidopsis thaliana, an antiflorigen protein, SP of tomato, CENTRORADIALIS (CEN) of gold flower, and Flowering locus T(FT) of Arabidopsis known as Florigen, of tomato The polypeptide sequences of proteins such as Single Flower Truss (SFT) were compared.

The orange boxed area represents the previously well-known joint with 14-3-3, and it can be seen that the same appears between heterogeneous species. The difference in the polypeptide sequence between the anti-flowering hormone and the flowering control hormone is indicated by a blue box. On the other hand, the substitution position of the base-substituted mutations (sp-classic, sp-5732 ) is indicated by a red box. In particular, in each case, it can be seen that the substituted base and polypeptide are well conserved regions between species.

This is a novel substitutional mutation ( sp-5732 ) disclosed in the present invention that enhances the production of other plants of the eggplant family, such as eggplant (Solanum melongena ), potato ( Solanum tuberosum ) tobacco genus ( Solanaceae Nicotiana ). It can be said to be an indicator that there is room for an important genetic resource that contributes to this.

7A to 7F are comparisons of the nucleotide sequences of the novel deletion mutants disclosed in the present invention. Based on the SP nucleotide sequence, it specifically shows at what position the base deletion of the deletion mutations (sp-2798, sp-2857) newly disclosed in the present invention occurs. The deletion site of each nucleotide sequence mutation is indicated by a red dotted line. sp- 2857 shows a mutant form in which 175bp of the first intron of the SP sequence is deleted. sp-2798 shows a mutant form in which 750 bp is deleted from the promoter portion of the SP sequence to the beginning of the first axon.

Figures 8a to 8b is a comparison of the gene coding portion (CDS) between the novel substitution and deletion mutations disclosed in the present invention. The part where the base has been substituted is indicated by a red box, and the part where the base has been deleted is indicated by a red dotted line. It can be seen that one base is substituted for both sp-classic and sp-5732. Specifically, when compared with the SP nucleotide sequence, sp-5732 can be confirmed that the 382th base is substituted from C to A. In the case of sp-2857 , it can also be confirmed that part of the second axon is deleted due to miss-splicing.

9 is a comparison of the sequences of polypeptides expressed by novel mutations disclosed in the present invention. Specifically, it contains information on the polypeptides of SEQ ID NOs: 6 and 7 expressed by the nucleotide sequences of SEQ ID NOs: 1 and 2. When the polypeptide of SEQ ID NO: 6 is compared with the sequence number of the polypeptide expressed by the SP nucleotide sequence, it can be seen that the 128th polypeptide is substituted from glutamine (Q) to lysine (K). The polypeptide of SEQ ID NO: 7 is a mutation in which the second axon is deleted due to miss-splicing, and it can be confirmed that 21 polypeptide sequences have been deleted.

The eggplant family disclosed in the present invention may be a purebred, hybrid, or inbred species on the premise that it contains the novel mutant autophagy gene. The eggplant family includes not only tomato plants, but also plants such as eggplant ( Solanum melongena ), potato ( Solanum tuberosum ) and tobacco genus ( Solanaceae Nicotiana ).

According to an embodiment of the present invention, the amount of production of the tomato plant may be increased by including the novel mutant self-arthrodesis gene in a tomato plant, especially among eggplants. The present invention includes all of the embodiments disclosed below. According to an embodiment of the present invention, the tomato plant may be characterized in that it simultaneously includes a nucleotide sequence of any one of SEQ ID NOs: 4 to 5 and a nucleotide sequence of any one of SEQ ID NOs: 1 to 3 . According to an embodiment of the present invention, the tomato plant may be a heterozygous comprising two base sequences of SEQ ID NOs: 1 to 3. For example, a specific example is a finite growth tomato plant that includes both the nucleotide sequence of SEQ ID NO: 1 and the nucleotide sequence of SEQ ID NO: 2. In addition, according to an embodiment of the present invention, the tomato plant may be homozygous including only any one nucleotide sequence of SEQ ID NOs: 1 to 3. For example, a specific example is a finite growth tomato plant containing only the nucleotide sequence of SEQ ID NO: 1.

All of the above-described tomato plants show the characteristics of delayed flowering time compared to the existing finite-growth tomato plants (sp-classic ) as the object of the present invention and the effect of increasing the production of tomatoes. By combining and utilizing the mutations disclosed in the present invention, it is possible to develop a finite growth type tomato plant having a variety of stem growth patterns. This means that, like the existing finite-growth tomato plants, it is possible to develop a tomato plant that guarantees multiple probabilities in accordance with various cultivation environments while maintaining the inherent advantages of the finite-growth tomato plant, which is labor reduction.

Hereinafter, more preferred embodiments of the present invention will be described in detail with reference to Examples. However, embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

{Example}

Example 1. The nucleotide sequence of the coding portion of the self-arthrodising gene in which a novel substitution mutation occurred (SEQ ID NO: 1, sp-5732 ).

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180

gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240

ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300

tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360

tttgtatttt tgctgtttaa gaagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420

agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480

gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga

Example 2. Base sequence of the coding portion of the magnetic arthropod gene in which the novel deletion mutation occurred (SEQ ID NO: 2, sp-2857 ).

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

gatctcagat ccttcttcac actggattgt cacagacatt ccaggcacta cagattgctc 180

ttttggaaga gaagtggttg ggtatgaaat gccaaggcca aatattggaa tccacaggtt 240

tgtatttttg ctgtttaagc agaagaaaag gcaaacaata tcgagtgcac cagtgtccag 300

agatcaattt agtagtagaa aattttcaga agaaaatgaa cttggctcac cagttgctgc 360

tgttttcttc aattgtcaga gggaaactgc cgctagaagg cgttga

Example 3. The base sequence of the self-removing gene in which a novel deletion mutation occurred (SEQ ID NO: 3, sp-2798 ).

acagttttct caatgatttt ttcttcttct tcatcttctc catcttctat tctcttcatc 60

ttcatagatt tagttacaga ttttcaataa ttcaacaacc atcaccatac tcacaattac 120

taccacctcc accatcacta tcaaccacta caatccttgc gatcaatctc tactacaaac 180

caatgaacca ttttcattat cataactagc acagctacta tcatcaacac atcatcaatt 240

accatatata ttcttcaccc atcgctgtta atatcactaa ctattaatat caatcaactt 300

caccaggaca atcaccatca ccactattaa atgtcatcat cacaccagtc attacaaaac 360

taacagtctc caacattacc agtaatcact aacaacaacc attattacaa acaatctcta 420

cttatttact tttattcaaa tatttattta gacaaaactg attttagtaa aacaaatgag 480

atcaatcttt ttctcgtgat taatttttaa gttggaatta gttccaaaat acatttaata 540

tagacaaata tgatactccc accgtcccat tttatgtaaa aaaacacgtc tcattttctt 600

atatggtaag tatttaaagg tataatttct cttttttacc tttattgatc ttaattttct 660

aatacattta tgagaagaga gaaaaatgag ttactttttt aaagaacgat ttgataaata 720

ttttaaaatc ttcattattt cttaaatttt gtgccagatc aaatgttgtc acataaaatg 780

agacggaaaa agtaaaacat atcaaacaca cccttaattt aaaatagtgt aggtactacc 840

taaaagtgga agttaattat ttgtttcccc aaaattaaat tttacccttg gacagcaatt 900

cctgtttaag ggttaattgt atagggacat acattttctt ctaatagtcc agggtagttt 960

ggttttcgat atgtggaaaa ttatctcgac ataaaatgct acagtaacta attagtacaa 1020

tatttagttt gtatttacca ttacatttag ctccacattc acataaattg gtagtacaac 1080

atttaatctt ctaatttgta ctataacatt ttcattaata aaaagtatgg tttctatctt 1140

caccattagc gtaacgttcg agtagagata tacatattta ttattataat atacgattgc 1200

aaatgccaaa gatggctaat tttgttttga gagactactg cattaagtaa attttttcag 1260

agacatgtat aagattaagt ctattgccaa ttctcaaata ttactctttt ttacttattg 1320

tggttattta tacatattaa gtgaactttc tttccttcct cagtaacttc taaacctagg 1380

gttgaagttc atggtggtga tctcagatcc ttcttcacac tggtatatat taatcttcaa 1440

cacttccaat ttactccgtc tgtctgtcct aatttatgtc acacattttc tatgatatat 1500

agttttagaa attattcaag accataactt tttaaagaaa aaatcataga ctttcttagt 1560

caacgtcaaa taaattgaga cggacaagat gacatgatta gtacatttat cttctattat 1620

tgacctctca ttttctttta tacattattt gacagatcat gatagatcca gatgttcctg 1680

gtcctagtga tccatatctc agggaacatc tacactggta tagacaacat atgccttaaa 1740

actaactcag tcaattttat cttcaattgt ttactttgga aggggaaatg acatgatcat 1800

tatatcatag tacaaattat tatgtaattt ctgttcgtct aaaaaatgtc actttagaaa 1860

aaactgataa tcatatacaa taccacaata aagatagaag aacatgtact aatattgaac 1920

ttaaataatg agtactagga gtattattaa ttaactttaa aaatgctagt caatatacct 1980

atgtttatat gttaaaaaat cctttatatt tggaaacatg agtactccta taccatacaa 2040

tgttgtcgta cagttgatta gacgggcaaa ttaaacaaat gtccaataat tgtactaatt 2100

aataactact tgttctcttc atctattatt agttattacc aaaaaaagag gactgcaaaa 2160

tggtgatatt attatgtgta acggaaaaaa acgtactcta tttaatatga tagaatcaaa 2220

gtgacatatt ttgttctagt tagacaaata agtaactgaa aagaggattt gaccatcttt 2280

acaggattgt cacagacatt ccaggcacta cagattgctc ttttggtatg tatccttaac 2340

ccataaatca aaataatgta ctttcttttt atttgccatt aatatctcta gtacaaaaaa 2400

gaaatattat aaaaaaaatt aatttcaatt tttatattat aggtttaaga taataatatt 2460

aaacgatatt ttagtctcta ccaaatagac gagcaaatta aaactaagaa agcactacat 2520

gttttcttta tattattagt ataaaaatat attataattt gcctggtggt aataggatca 2580

aagtattgat tcttaattat tattatataa ttaataataa tggtaaacaa aaagatataa 2640

agtgcttacc tcctaattcc ctatatgaaa aaatatactt acttaattac tctttttaca 2700

cgtaagcatg catttaaaaa aatattaaaa aattattcca gaggttatat ataatatgta 2760

tggataaaaa aaaaattcac ctatatacat aataatataa ttttcgagtg aattgaccgc 2820

ccttcagcat cattatataa tgttatcgat ctaggtcttt gtgtgaaatt aaaagttatt 2880

tatacggtta gtacgatcgc gtaataacga aggtaaaaat atttcaggaa gagaagtggt 2940

tgggtatgaa atgccaaggc caaatattgg aatccacagg tttgtatttt tgctgtttaa 3000

gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc agagatcaat ttagtagtag 3060

aaaattttca gaagaaaatg aacttggctc accagttgct gctgttttct tcaattgtca 3120

gagggaaact gccgctagaa ggcgttga

Comparative Example 1. The nucleotide sequence of the coding portion of the auto-arthrodesis gene in which the conventional substitution mutation occurred (SEQ ID NO: 5, sp-classic )

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180

gatctcagat ccttcttcac actgatcatg atagatccag atgttcttgg tcctagtgat 240

ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300

tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360

tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420

agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480

gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga

{evaluation}

1. Comparison of number of leaves per stem of sympodial

In the finite growth tomato plants including the nucleotide sequences of Examples 1 to 3, it was found that the number of leaves per stem was different for each plant. The difference in the number of leaves indicates that the time it takes to harvest tomatoes is different. This suggests that the novel mutations disclosed in the present invention are important genetic resources for diversifying the cultivation period of tomato plants as the present invention aims. (See Fig. 4)

2. Comparison of the total number of flowers

When compared with Comparative Example 1, the finite growth type tomato plants including the nucleotide sequences of Examples 1 to 3 showed an increase in the average number of flower stalks. It can be inferred that as the number of flower stalks increases, the production of tomatoes will also increase. In particular, in the case of the finite-growth tomato plant including the nucleotide sequence of Example 3, it was found that the number of flowers increased by 1.5 times or more compared to the existing finite-growth tomato plant. This means that the yield of the finite-growth tomato plant including the nucleotide sequences of Examples 1 to 3 disclosed in the present invention is advantageous in terms of high probability compared to the existing finite-growth tomato plant. (See Fig. 5)

3. Comprehensive evaluation

In light of the above, by utilizing the nucleotide sequences of Examples 1 to 3, it is possible to diversify the cultivation period of the finite-growth tomato plant and achieve multiple probability. Therefore, the finite-growth tomato plant including the nucleotide sequence can be cultivated economically even in facility cultivation while preserving the labor-reduction effect that can be said to be an advantage of the existing finite-growth tomato plant.

Genotype Mutation CC lines # sp-5732
(Example 1, SEQ ID NO: 1) substitution 5732, 6257, 6258, 6259 sp-2857
(Example 2, SEQ ID NO: 2) deletion 871, 1679, 2798, 3249, 3441, 3480 sp-2798
(Example 3, SEQ ID NO: 3) deletion 873, 2857, 3186, 879, 2271, 3234 sp-classic
(Comparative Example 1) substitution

<110> Wonkwang University Center for Industry-Academy Cooperation <120> A Novel mutant tomato plants with modified determinate shoot architecture <130> KL-P-01865 <160> 9 <170> KoPatentIn 3.0 <210> 1 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 1 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gaagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 2 <211> 406 <212> DNA <213> Lycopersicon esculentum <400> 2 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 gatctcagat ccttcttcac actggattgt cacagacatt ccaggcacta cagattgctc 180 ttttggaaga gaagtggttg ggtatgaaat gccaaggcca aatattggaa tccacaggtt 240 tgtatttttg ctgtttaagc agaagaaaag gcaaacaata tcgagtgcac cagtgtccag 300 agatcaattt agtagtagaa aattttcaga agaaaatgaa cttggctcac cagttgctgc 360 tgttttcttc aattgtcaga gggaaactgc cgctagaagg cgttga 406 <210> 3 <211> 3148 <212> DNA <213> Lycopersicon esculentum <400> 3 acagttttct caatgatttt ttcttcttct tcatcttctc catcttctat tctcttcatc 60 ttcatagatt tagttacaga ttttcaataa ttcaacaacc atcaccatac tcacaattac 120 taccacctcc accatcacta tcaaccacta caatccttgc gatcaatctc tactacaaac 180 caatgaacca ttttcattat cataactagc acagctacta tcatcaacac atcatcaatt 240 accatatata ttcttcaccc atcgctgtta atatcactaa ctattaatat caatcaactt 300 caccaggaca atcaccatca ccactattaa atgtcatcat cacaccagtc attacaaaac 360 taacagtctc caacattacc agtaatcact aacaacaacc attattacaa acaatctcta 420 cttatttact tttattcaaa tatttattta gacaaaactg attttagtaa aacaaatgag 480 atcaatcttt ttctcgtgat taatttttaa gttggaatta gttccaaaat acatttaata 540 tagacaaata tgatactccc accgtcccat tttatgtaaa aaaacacgtc tcattttctt 600 atatggtaag tatttaaagg tataatttct cttttttacc tttattgatc ttaattttct 660 aatacattta tgagaagaga gaaaaatgag ttactttttt aaagaacgat ttgataaata 720 ttttaaaatc ttcattattt cttaaatttt gtgccagatc aaatgttgtc acataaaatg 780 agacggaaaa agtaaaacat atcaaacaca cccttaattt aaaatagtgt aggtactacc 840 taaaagtgga agttaattat ttgtttcccc aaaattaaat tttacccttg gacagcaatt 900 cctgtttaag ggttaattgt atagggacat acattttctt ctaatagtcc agggtagttt 960 ggttttcgat atgtggaaaa ttatctcgac ataaaatgct acagtaacta attagtacaa 1020 tatttagttt gtatttacca ttacatttag ctccacattc acataaattg gtagtacaac 1080 atttaatctt ctaatttgta ctataacatt ttcattaata aaaagtatgg tttctatctt 1140 caccattagc gtaacgttcg agtagagata tacatattta ttattataat atacgattgc 1200 aaatgccaaa gatggctaat tttgttttga gagactactg cattaagtaa attttttcag 1260 agacatgtat aagattaagt ctattgccaa ttctcaaata ttactctttt ttacttattg 1320 tggttattta tacatattaa gtgaactttc tttccttcct cagtaacttc taaacctagg 1380 gttgaagttc atggtggtga tctcagatcc ttcttcacac tggtatatat taatcttcaa 1440 cacttccaat ttactccgtc tgtctgtcct aatttatgtc acacattttc tatgatatat 1500 agttttagaa attattcaag accataactt tttaaagaaa aaatcataga ctttcttagt 1560 caacgtcaaa taaattgaga cggacaagat gacatgatta gtacatttat cttctattat 1620 tgacctctca ttttctttta tacattattt gacagatcat gatagatcca gatgttcctg 1680 gtcctagtga tccatatctc agggaacatc tacactggta tagacaacat atgccttaaa 1740 actaactcag tcaattttat cttcaattgt ttactttgga aggggaaatg acatgatcat 1800 tatatcatag tacaaattat tatgtaattt ctgttcgtct aaaaaatgtc actttagaaa 1860 aaactgataa tcatatacaa taccacaata aagatagaag aacatgtact aatattgaac 1920 ttaaataatg agtactagga gtattattaa ttaactttaa aaatgctagt caatatacct 1980 atgtttatat gttaaaaaat cctttatatt tggaaacatg agtactccta taccatacaa 2040 tgttgtcgta cagttgatta gacgggcaaa ttaaacaaat gtccaataat tgtactaatt 2100 aataactact tgttctcttc atctattatt agttattacc aaaaaaagag gactgcaaaa 2160 tggtgatatt attatgtgta acggaaaaaa acgtactcta tttaatatga tagaatcaaa 2220 gtgacatatt ttgttctagt tagacaaata agtaactgaa aagaggattt gaccatcttt 2280 acaggattgt cacagacatt ccaggcacta cagattgctc ttttggtatg tatccttaac 2340 ccataaatca aaataatgta ctttcttttt atttgccatt aatatctcta gtacaaaaaa 2400 gaaatattat aaaaaaaatt aatttcaatt tttatattat aggtttaaga taataatatt 2460 aaacgatatt ttagtctcta ccaaatagac gagcaaatta aaactaagaa agcactacat 2520 gttttcttta tattattagt ataaaaatat attataattt gcctggtggt aataggatca 2580 aagtattgat tcttaattat tattatataa ttaataataa tggtaaacaa aaagatataa 2640 agtgcttacc tcctaattcc ctatatgaaa aaatatactt acttaattac tctttttaca 2700 cgtaagcatg catttaaaaa aatattaaaa aattattcca gaggttatat ataatatgta 2760 tggataaaaa aaaaattcac ctatatacat aataatataa ttttcgagtg aattgaccgc 2820 ccttcagcat cattatataa tgttatcgat ctaggtcttt gtgtgaaatt aaaagttatt 2880 tatacggtta gtacgatcgc gtaataacga aggtaaaaat atttcaggaa gagaagtggt 2940 tgggtatgaa atgccaaggc caaatattgg aatccacagg tttgtatttt tgctgtttaa 3000 gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc agagatcaat ttagtagtag 3060 aaaattttca gaagaaaatg aacttggctc accagttgct gctgttttct tcaattgtca 3120 gagggaaact gccgctagaa ggcgttga 3148 <210> 4 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 4 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 5 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 5 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttcttgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 6 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 6 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly 1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr 20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser 35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser 50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Pro Gly Pro Ser Asp 65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly 85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro 100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Lys 115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe 130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg 165 170 175 <210> 7 <211> 154 <212> PRT <213> Lycopersicon esculentum <400> 7 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly 1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr 20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser 35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser 50 55 60 Phe Phe Thr Leu Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Cys Ser 65 70 75 80 Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro Arg Pro Asn Ile Gly 85 90 95 Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln Lys Lys Arg Gln Thr 100 105 110 Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe Ser Ser Arg Lys Phe 115 120 125 Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala Ala Val Phe Phe Asn 130 135 140 Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg 145 150 <210> 8 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 8 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly 1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr 20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser 35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser 50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Pro Gly Pro Ser Asp 65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly 85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro 100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln 115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe 130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg 165 170 175 <210> 9 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 9 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly 1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr 20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser 35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser 50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Leu Gly Pro Ser Asp 65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly 85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro 100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln 115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe 130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg 165 170 175

Claims (5)

In genetically modified finite-grown tomato plants,
The genetically modified finite-grown tomato plant comprises a mutant self-arranging gene, wherein the mutant self-arthroening gene comprises a nucleotide sequence of SEQ ID NO: 2. Genetically modified finite-grown tomato plant.
The method of claim 1,
The genetically modified finite-grown tomato plant is characterized in that the mutant self-arrangement gene encodes the polypeptide of SEQ ID NO: 7.
The method according to claim 1 or 2,
The genetically modified finite growth tomato plant is genetically modified finite growth tomato plant, characterized in that obedience.
The method according to claim 1 or 2,
The genetically modified finite growth tomato plant is genetically modified finite growth tomato plant, characterized in that the inbred hybrid.
The method according to claim 1 or 2,
The genetically modified finite-grown tomato plant is a genetically modified finite-grown tomato plant, characterized in that hybrid.

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