KR101983902B1 - A Novel mutant tomato plants with modified determinate shoot architecture - Google Patents

Abstract

The present invention relates to a genetically modified finely grown tomato plant, wherein the genetically modified finely grown tomato plant comprises a mutant self-mutation gene, wherein the mutant self-mutation gene has a sequence identity of SEQ ID NO: 1 to SEQ ID NO: : 3 < / RTI >

Description

[0001] The present invention relates to a novel mutant tomato plant having improved finite stem structure,

The present invention relates to a novel mutant tomato plant having improved finite growth stem structure. More preferably, the present invention relates to a finely grown tomato plant having a multiple cropping property.

Tomatoes are one of the most consumed vegetables in the world, and they are often regarded as healthy vegetables. As the interest in tomatoes grows, the cultivation environment has also diversified. Typical tomato cultivation environments include open field cultivation and plant cultivation.

Facility cultivation is a cultivation method that utilizes facilities such as a greenhouse. It has the advantage of being able to keep the cultivation environment constant compared with the cultivation of the non-cultivated land, but it has a disadvantage that the number of flowers per plant is small. Typically, tomato varieties of Determinate (D) type are preferred for non-growing, and indeterminate (ID) tomato varieties are preferred for cultivation.

After the self-pruning (sp ) mutation of the finely grown tomatoes was discovered in the last century, several breeders tried to control the growth pattern and flowering pattern by utilizing the mutation as a breeding material. Furthermore, a study of sp mutants revealed that the protein encoded by the self-spreading gene ( sp gene ) is an antiflorigen , one of the regulated hormones, Is also known to play an important role in the stem growth and nutrient growth cycle of tomatoes.

The finely grown tomato varieties are known as effective varieties for labor saving. In addition, the existing hybrids study confirms that it is possible to obtain a high degree of accuracy of the finely grown tomato varieties by regulating the stem growth period. Specifically, a method of increasing the yield of tomato plants by increasing the number of stem (symmetrical) sympodial stem is often used as a method of ensuring the plurality of elongation.

However, the existing finely grown tomato varieties were not successful in diversifying stem growth patterns using only a single mutation, which means that diversity of cultivation period suitable for each cultivation environment can not be guaranteed either. In addition, finned growth type tomatoes were also a serious problem in terms of lack of economic efficiency compared to labor reliance in cultivation in facilities such as greenhouses.

U. S. Patent No. 9,157, 093 B2

In order to solve the above-mentioned problems, it is an object of the present invention to provide a mutant finely grown tomato plant which has a variety of stem growth patterns, can ensure diversity of cultivation period,

In order to achieve the above object, the present invention discloses a genetically modified finely grown tomato plant.

The genetically modified finite-growth tomato plant of the present invention comprises a mutant self-mutation gene, and the mutant self-mutation gene may include a nucleotide sequence of SEQ ID NO: 1 ( sp-5732 ).

The genetically modified finite growth tomato plant of the present invention comprises a mutant self-mutation gene, and the mutant self-mutation gene can be substituted with C at position 382 of the nucleotide sequence of SEQ ID NO: 4 ( SP ).

The genetically modified finitely grown tomato plant of the present invention comprises a mutant self-mutation gene, wherein the mutant self mutation gene can encode the polypeptide of SEQ ID NO: 6.

The genetically modified finely grown tomato plant of the present invention is obedient. The genetically modified finely grown tomato plant of the present invention is a hybrid. This genetically modified finely grown tomato plant is an inbreed hybrid.

The finely grown tomato varieties modified by the self-mutation gene of the present invention as described above provide an effect of reducing the labor force and increasing the production amount of tomato.

In addition, the present invention provides a tomato varieties with a deficient self-knotting gene of the present invention, in which stem growth patterns are different from those of conventional tomato varieties, and the number of flower buds is increased to secure a large number of varieties.

Also, the finely grown tomato varieties in which some of the nucleotide sequences of the magnetic arthropods of the present invention are substituted are provided, and the tomato varieties having different stem growth patterns from those of the existing tomato varieties and having increased number of flower buds and securing a large number of varieties are provided.

Fig. 1 shows the results of electrophoresis on the mutation of the self-elongation gene disclosed in the present invention.
FIG. 2 is a conceptual diagram showing the gene structure of the newly developed self-mutation mutants in the present invention.
Fig. 3 is a conceptual diagram classified into stem growth types of tomato plants. Fig.
4 is a conceptual diagram showing the number of leaves of sympodial stem according to the mutation type.
5 is a graph showing the total number of inflorescence of self-mutation gene mutations.
Figure 6 compares polypeptide sequences between SP homologs, including self-mutant gene mutations.
Figures 7A-7F compare the nucleotide sequences of the novel deletion mutants disclosed in the present invention.
Figures 8A-8B compare the Coding Sequence (CDS) between the novel substitutions and deletion mutations disclosed in the present invention.
Figure 9 compares the sequences of the polypeptides expressed by the novel mutations disclosed in the present invention.

The invention disclosed herein relates to a mutation of the self-pruning gene ( sp gene ) of a plant with a finite growth-type branch, preferably Solanum lycopersicum . A finite growth type tomato plant containing a mutation of a novel self-mutation gene appears to have increased production, preferably tomato production, compared to the conventional finely grown tomato plant.

The self - mutation gene is a gene that regulates the stem growth period of tomato plants. Mutation of the self - mutation gene appears as a change in stem growth pattern of tomato plant. The self-mutation gene encodes the antiflorigen , and the anti- inflammatory hormone is known as the inhibitor of flowering. The specific nucleotide sequence of the SP wild-type as a self-arginine gene without mutation is the same as the nucleotide sequence ( SP ) of SEQ ID NO: 4.

The endless growth type (ID) is a growth type in which the occurrence and growth of the sympodial stem is continuous and repeated, which is advantageous from the viewpoint of the graininess, but requires a considerable labor force for sowing and harvesting. Tomato plants containing the nucleotide sequence of SEQ ID NO: 4 generally grow to infinite growth-type stems. The polypeptide encoded by the nucleotide sequence of SEQ ID NO: 4 is the SP wild-type polypeptide and is as shown in SEQ ID NO: 8.

The finite growth (D) type means that the occurrence of the sympodial is not continuous and its growth style is not repetitive. The nucleotide sequence well known as the mutant self-mutation gene is the same as the nucleotide sequence of SEQ ID NO: 5 ( sp classic ). The above-mentioned base sequence ( sp classic ) is the same as the base sequence in which the 228th base of SEQ ID NO: 4 is substituted with C to T. Due to the substitution of the nucleotide sequence, tomato plants become finite growth type.

The finely grown tomato plants have the effect of reducing the labor force in sowing and harvesting, but they are disadvantageous in terms of economy because the yield of tomato per plant is less than that of infinite growth type. Especially, it is considered that the number of plants per plant is absolutely insufficient when cultivating plants such as a greenhouse, resulting in lack of economic efficiency.

In particular, the existing finned growth tomatoes utilize only a single variant factor ( sp classic ), so that the variety of tomato plant cultivation period is not guaranteed, and the problem that the above- It remained.

The novel self-mutation gene mutations disclosed in the present invention are different from existing mutant genes and express various finite stem-type stem cells. The gene mutants that play a key role in securing the large- It is a circle. Specifically, it includes one substitution mutation and two deletion mutations.

The process for ensuring the new mutation is as follows.

First, we have 210 lines of finite growth and 26 lines of semi-determinate (SD) to collect the core mutation groups. The presence of a mutation on the SP gene (SEQ ID NO: 4) from the ensured line is checked.

Specific methods for determining whether a mutation has occurred on the gene are as follows. First, the well known sp mutation ( sp classic ) is isolated and excluded by genotyping. Genetic expression studies (RT-PCR) are conducted to investigate the genotype of lines showing SP genotype. It also identifies the nucleotide sequence and protein of the gene coding portion (CDS).

As a result, it was confirmed that 4 mutants were substituted with one base on the SP gene, and all of the 4 lines had the same substitution mutation (SEQ ID NO: 1, sp-5732 ). In addition, six lines with deletion mutations were found in the SP promoter region and all six lines were identified as the same deletion mutant (SEQ ID NO: 2, sp-2857 ). In addition, six lines with deletion mutations in the first intron region were obtained, and all six lines were identified as the same deletion mutant (SEQ ID NO: 3, sp-2798 ).

In order to make the genomic background of the newly obtained mutant core population identical, M82 tomato cultivars were subjected to reverse crossing more than 6 times. Also cv. In the background of M82, the stem growth patterns between sp classic and new mutants were compared and analyzed. Table 1 summarizes the new mutations in the self-mutation gene. The CC lines # in Table 1 indicate the number of the specific plant in which the mutation was found.

Hereinafter, more preferred embodiments of the present invention will be described in detail with reference to the drawings. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments. It is to be understood that both the foregoing general description and the following detailed description of the present invention are exemplary and explanatory and are intended to provide further explanation of the invention to those skilled in the art. .

According to one embodiment of the present invention, the genetically modified finely grown tomato plant comprises a novel mutant self-knockout gene, wherein the mutant self knockout gene comprises the nucleotide sequence of SEQ ID NO: 1 ( sp-5732 ) . SEQ ID NO: 1 corresponds to the coding portion (CDS) of the mutant self-mutation gene. The nucleotide sequence of SEQ ID NO: 1 encodes the polypeptide of SEQ ID NO: 6. The polypeptide of SEQ ID NO: 6 is the sp-5732 amino acid. According to another embodiment of the present invention, the genetically modified finely grown tomato plant comprises a novel mutant self-knockout gene, wherein the mutant self knockout gene comprises the nucleotide sequence ( sp-2857 ) of SEQ ID NO: 2 . The nucleotide sequence of SEQ ID NO: 2 encodes the polypeptide of SEQ ID NO: 7. The polypeptide of SEQ ID NO: 7 is sp-2857 amino acid. SEQ ID NO: 2 corresponds to the coding portion (CDS) of the mutant self-mutation gene. According to another embodiment of the present invention, a genetically modified finely grown tomato plant comprises a novel mutant self-knockout gene, wherein the mutant self knockout gene has a nucleotide sequence of SEQ ID NO: 3 ( sp-2798 ) .

According to an embodiment of the present invention, the genetically modified finite-growth tomato plant includes a mutant self-mutation gene, and the mutant self-mutation gene encodes a polypeptide of SEQ ID NO: 6. According to an embodiment of the present invention, the genetically modified finely grown tomato plant includes a mutant self-mutation gene, and the mutant self mutation gene is characterized by encoding the polypeptide of SEQ ID NO: 7.

In addition, the base sequence ( SP ) of SEQ ID NO: 4 encodes the polypeptide of SEQ ID NO: 8. The nucleotide sequence represented by SEQ ID NO: 4 is the SP wild-type nucleotide sequence. The polypeptide of SEQ ID NO: 8 is a SP wild-type amino acid. The nucleotide sequence ( SP ) of SEQ ID NO: 4 is the coding region (CDS) of the self-elongation gene. The nucleotide sequence of SEQ ID NO: 5 ( sp-classic ) encodes the polypeptide of SEQ ID NO: 9. The nucleotide sequence of SEQ ID NO: 5 is the sp-classic nucleotide sequence. The polypeptide of SEQ ID NO: 9 is an sp-classic amino acid. The nucleotide sequence of SEQ ID NO: 5 ( sp-classic ) is the coding region (CDS) of the self - elongation gene.

Fig. 1 shows the results of electrophoresis on the mutation of the self-elongation gene disclosed in the present invention. Referring to FIG. 1, new mutations ( sp-2857 and sp-5732 ) of the self - mutation gene can be confirmed primarily by electrophoresis. SP (SEQ ID NO: 4) markers and the conventional mutants sp-classic (SEQ ID NO: 5) was compared with the marker, and as a result of forming the both sp-2857 and sp-5732 SP and sp-classic with different band . Especially for sp-5732 , bands are formed at positions similar to SP , which grows infinitely though it is a finite growth type.

FIG. 2 is a conceptual diagram showing the gene structure of the newly developed self-mutation mutants in the present invention. sp-2798 and sp-2857 represent a newly developed deletion mutation. In the drawing, it is a dotted line portion. In sp-2857 , deletion occurred in the first intron region, and sp-2798 can be confirmed that deletion occurred in the promoter region.

The yellow box in FIG. 2 is a maxon (EXON), and the white box represents a non-translation zone. A white box drawn with a dashed line represents the missing part of the non-translation area. The new substitution mutation, sp-5732 , can be confirmed to have been replaced by a different axon than the conventional fin-grown mutant ( sp-classic ). In addition, blue lines represent mis-spliced RNA.

Fig. 3 is a conceptual diagram classified into stem growth types of tomato plants. Fig. The black and gray bars indicate successive stems. The black and gray ellipses indicate the leaves that occur on the stem, and the numbers on the leaves represent the number of leaves that have occurred. The arrows indicate the side, and the green bars indicate the pedicles. The green, yellow, and red circles are a rough representation of fruit maturity, and yellow stars are flowers.

Tomato plants containing the nucleotide sequence (SP) of SEQ ID NO: 4 generally express infinite growth (ID) stems. The first occurring stem produces 8 to 9 leaves and the growth point is terminated by the occurrence of flower buds and flowers. The subsequent sympodial stem generates three leaves, and again the flower buds and flowers are generated, resulting in the end of the growing point. In the case of infinite growth tomato plants, the occurrence, growth and termination of the above-mentioned Sympodial stem are continuously and repeatedly performed. It is economically superior because the production of continuous livestock stalks and the production of tomatoes in a single plant.

On the other hand, tomato plants containing the nucleotide sequence of SEQ ID NO: 5 ( sp-classic ) generally express finite growth (D) type stems. After the first stem grows 7 to 9 leaves, the flower buds and flowers are produced and the growth point is terminated. However, unlike the inflorescence-growing tomato plant, the subsequent sympodial stem gradually decreases the number of leaves from 3 to 0 and eventually ends the generation of the sympodial stem. Since stem growth is not over a certain level, it is advantageous that sowing and tomatoing are easy to harvest.

Semi finite growth (SD) type tomato plants show a delayed termination of the development of sympodial stem compared to finite growth type tomato plants. Therefore, it is characterized by showing the stem structure of the form of increased number of flower buds. In general, a method for delaying the termination of the development of the sympodial stem, such as the semi-finite growth tomato plant, is preferred as a method for improving the yield of the finely grown tomato plant.

4 is a conceptual diagram showing the number of leaves of sympodial stem according to the mutation type. Specifically, the number of leaves and the number of the sympodial stem of the existing mutant tomato plant of the present invention disclosed in the present invention are compared with those of the existing fin-grown mutant ( sp-classic ) tomato plant. In the graph, black and gray indicate the number of leaves formed per Sympodial stem, respectively. The number of leaves indirectly indicates the flowering time of each of the sympodial stems.

sp-2798 and sp- 2857 show the appearance of sympodial stem similar to the existing finite growth mutation. However, it can be confirmed that the number of leaves is increased compared to the existing finite growth mutants, and it can be deduced that the flowering is delayed. In particular, sp-5732 is delayed in the end of the further development of the sympodial stem, indicating that the stem growth pattern is similar to that of the finely grown tomato plant. This means that the tomato plant of sp-5732 is more advantageous than the conventional finely grown mutant tomato plant in ensuring the high degree of certainty.

5 is a graph showing the total number of inflorescence of self-mutation gene mutations. It can be inferred that as the number of flowers increases, the yield of tomato per unit plant increases. Specifically, it is a graph comparing the number of flower beds of mutant tomato plants grown for 3 months after transplanting. The numbers of spikelets were increased in all tomato plants including sp-2798 , sp-2857 and sp-5732 compared with the existing finite growth mutants. In particular, the number of spikelets in the tomato plants including sp-5732 was significantly increased.

Figure 6 compares polypeptide sequences between SP homologs, including self-mutant gene mutations. Specifically, SP homologs refer to several species in which the SP base sequence of the common ancestor is inherited. Terminal Flower 1 of Arabidopsis thaliana wherein the blooming hormone (Antiflorigen) protein (TFL1), Flowering locus T ( FT) of the tomato SP, gold plants known as CENTRORADIALIS (CEN), and flowering hormone (Florigen) Arabidopsis thaliana, tomato Single Flower Truss (SFT).

The orange box region indicates the binding site with the well-known 14-3-3, and it can be confirmed that the same is observed in the other species. The difference in the polypeptide sequence between anti-androgenic hormones is indicated by the blue box. On the other hand, the substitution position of the base-substituted mutants ( sp-classic, sp-5732 ) is indicated by a red box. In particular, in each case, the substituted base and the polypeptide can be confirmed as well-conserved regions.

This is because the novel mutation ( sp-5732 ) disclosed in the present invention enhances the yields for plants such as Solanum melongena , Solanum tuberosum tobacco ( Solanaceae Nicotiana ) This is an indicator that there is room to become an important genetic resource contributing to

Figures 7A-7F compare the nucleotide sequences of the novel deletion mutants disclosed in the present invention. ( Sp-2798, sp-2857 ) , which is newly disclosed in the present invention, based on the SP base sequence, in which position the base deletion of the newly deleted deletion mutants ( sp-2798, sp-2857 ) occurs. The deletion site of each nucleotide sequence mutation is indicated by a red dotted line. sp- 2857 shows a mutant form in which 175 bp of the base sequence of the first intron of the SP nucleotide sequence has been deleted. sp-2798 shows a mutant form with 750 bp deletion from the promoter region of the SP base sequence to the start of the first axon.

Figures 8a-8b compare the gene coding portions (CDS) between the novel replacement and deletion mutants disclosed in the present invention. Replacement of the base is indicated by a red box, and the area where the base is deleted is indicated by a red dotted line. Both sp-classic and sp-5732 can be confirmed to be substituted with one base. Specifically, when sp-5732 is compared with the SP base sequence, it can be confirmed that the 382nd base is substituted with A in C. In the case of sp-2857 , it is also confirmed that some of the second axons are lost due to mis-splicing.

Figure 9 compares the sequences of the polypeptides expressed by the novel mutations disclosed in the present invention. Specifically, it contains information on the polypeptides of SEQ ID NOS: 6 and 7 expressed by the nucleotide sequences of SEQ ID NOS: 1 and 2. When the polypeptide of SEQ ID NO: 6 is compared with the sequence number of the polypeptide expressed by the SP base sequence, it can be confirmed that the 128th polypeptide is substituted with lysine (K) in glutamine (Q). The polypeptide of SEQ ID NO: 7 is a mutant in which the second axon is deleted due to mis-splicing, indicating that 21 polypeptide sequences have been deleted.

The branches and plants disclosed in the present invention may be pure, hybrid, or inbreeding hybrid on the assumption that they contain the novel mutant self-mutation gene. Branches not only contain tomato plants, but also include plants such as Solanum melongena and Solanum tuberosum tobacco ( Solanaceae Nicotiana ).

According to one embodiment of the present invention, the tomato mutant self-mutation gene is contained in the tomato plant, and the yield of the tomato plant can be increased. The present invention includes all of the embodiments disclosed below. According to an embodiment of the present invention, the tomato plant may include a base sequence of any one of SEQ ID NOS: 4 to 5 and a base sequence of any one of SEQ ID NOS: 1 to 3 . According to one embodiment of the present invention, the tomato plant may be a heterozygous sequence comprising two nucleotide sequences of SEQ ID NO: 1 to 3. For example, a specific example is a finely grown tomato plant comprising the nucleotide sequence of SEQ ID NO: 1 and the nucleotide sequence of SEQ ID NO: 2. According to an embodiment of the present invention, the tomato plant may be a homozygous junction comprising only a base sequence of any one of SEQ ID NOS: 1 to 3. For example, a specific example is a finely grown tomato plant comprising only the nucleotide sequence of SEQ ID NO: 1.

As described above, the tomato plants described above are characterized in that the flowering time is delayed and the production amount of tomato is also increased as compared with the existing fin- grown tomato plants ( sp-classic ). By combining and utilizing the mutations disclosed in the present invention, it is possible to develop a finely grown tomato plant having various stem growth patterns. This means that tomato plants can be developed that can guarantee diversity in various cultivation environments while maintaining the inherent advantages of finite growth type tomato plants such as labor-saving reduction, as in the case of the existing finite growth tomato plants.

Hereinafter, more preferred embodiments of the present invention will be described in detail with reference to examples. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments.

{Example}

Example 1: The nucleotide sequence of the coding portion of the self-knockout gene (SEQ ID NO: 1, sp-5732 ).

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180

gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240

ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300

tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360

tttgtatttt tgctgtttaa gaagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420

agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480

gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga

Example 2: The nucleotide sequence of the coding portion of the self-arginine gene (SEQ ID NO: 2, sp-2857 ).

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

gatctcagat ccttcttcac actggattgt cacagacatt ccaggcacta cagattgctc 180

ttttggaaga gaagtggttg ggtatgaaat gccaaggcca aatattggaa tccacaggtt 240

tgtatttttg ctgtttaagc agaagaaaag gcaaacaata tcgagtgcac cagtgtccag 300

agatcaattt agtagtagaa aattttcaga agaaaatgaa cttggctcac cagttgctgc 360

tgttttcttc aattgtcaga gggaaactgc cgctagaagg cgttga

Example 3: A nucleotide sequence of a self-elongation gene in which a novel deletion mutation occurred (SEQ ID NO: 3, sp-2798 ).

acagttttct caatgatttt ttcttcttct tcatcttctc catcttctat tctcttcatc 60

ttcatagatt tagttacaga ttttcaataa ttcaacaacc atcaccatac tcacaattac 120

taccacctcc accatcacta tcaaccacta caatccttgc gatcaatctc tactacaaac 180

caatgaacca ttttcattat cataactagc acagctacta tcatcaacac atcatcaatt 240

accatatata ttcttcaccc atcgctgtta atatcactaa ctattaatat caatcaactt 300

caccaggaca atcaccatca ccactattaa atgtcatcat cacaccagtc attacaaaac 360

taacagtctc caacattacc agtaatcact aacaacaacc attattacaa acaatctcta 420

cttatttact tttattcaaa tatttattta gacaaaactg attttagtaa aacaaatgag 480

atcaatcttt ttctcgtgat taatttttaa gttggaatta gttccaaaat acatttaata 540

tagacaaata tgatactccc accgtcccat tttatgtaaa aaaacacgtc tcattttctt 600

atatggtaag tatttaaagg tataatttct cttttttacc tttattgatc ttaattttct 660

aatacattta tgagaagaga gaaaaatgag ttactttttt aaagaacgat ttgataaata 720

ttttaaaatc ttcattattt cttaaatttt gtgccagatc aaatgttgtc acataaaatg 780

agacggaaaa agtaaaacat atcaaacaca cccttaattt aaaatagtgt aggtactacc 840

taaaagtgga agttaattat ttgtttcccc aaaattaaat tttacccttg gacagcaatt 900

cctgtttaag ggttaattgt atagggacat acattttctt ctaatagtcc agggtagttt 960

ggttttcgat atgtggaaaa ttatctcgac ataaaatgct acagtaacta attagtacaa 1020

tatttagttt gtatttacca ttacatttag ctccacattc acataaattg gtagtacaac 1080

atttaatctt ctaatttgta ctataacatt ttcattaata aaaagtatgg tttctatctt 1140

caccattagc gtaacgttcg agtagagata tacatattta ttattataat atacgattgc 1200

aaatgccaaa gatggctaat tttgttttga gagactactg cattaagtaa attttttcag 1260

agacatgtat aagattaagt ctattgccaa ttctcaaata ttactctttt ttacttattg 1320

tggttattta tacatattaa gtgaactttc tttccttcct cagtaacttc taaacctagg 1380

gttgaagttc atggtggtga tctcagatcc ttcttcacac tggtatatat taatcttcaa 1440

cacttccaat ttactccgtc tgtctgtcct aatttatgtc acacattttc tatgatatat 1500

agttttagaa attattcaag accataactt tttaaagaaa aaatcataga ctttcttagt 1560

caacgtcaaa taaattgaga cggacaagat gacatgatta gtacatttat cttctattat 1620

tgacctctca ttttctttta tacattattt gacagatcat gatagatcca gatgttcctg 1680

gtcctagtga tccatatctc agggaacatc tacactggta tagacaacat atgccttaaa 1740

actaactcag tcaattttat cttcaattgt ttactttgga aggggaaatg acatgatcat 1800

tatatcatag tacaaattat tatgtaattt ctgttcgtct aaaaaatgtc actttagaaa 1860

aaactgataa tcatatacaa taccacaata aagatagaag aacatgtact aatattgaac 1920

ttaaataatg agtactagga gtattattaa ttaactttaa aaatgctagt caatatacct 1980

atgtttatat gttaaaaaat cctttatatt tggaaacatg agtactccta taccatacaa 2040

tgttgtcgta cagttgatta gacgggcaaa ttaaacaaat gtccaataat tgtactaatt 2100

aataactact tgttctcttc atctattatt agttattacc aaaaaaagag gactgcaaaa 2160

tggtgatatt attatgtgta acggaaaaaa acgtactcta tttaatatga tagaatcaaa 2220

gtgacatatt ttgttctagt tagacaaata agtaactgaa aagaggattt gaccatcttt 2280

acaggattgt cacagacatt ccaggcacta cagattgctc ttttggtatg tatccttaac 2340

ccataaatca aaataatgta ctttcttttt atttgccatt aatatctcta gtacaaaaaa 2400

gaaatattat aaaaaaaatt aatttcaatt tttatattat aggtttaaga taataatatt 2460

aaacgatatt ttagtctcta ccaaatagac gagcaaatta aaactaagaa agcactacat 2520

gttttcttta tattattagt ataaaaatat attataattt gcctggtggt aataggatca 2580

aagtattgat tcttaattat tattatataa ttaataataa tggtaaacaa aaagatataa 2640

agtgcttacc tcctaattcc ctatatgaaa aaatatactt acttaattac tctttttaca 2700

cgtaagcatg catttaaaaa aatattaaaa aattattcca gaggttatat ataatatgta 2760

tggataaaaa aaaaattcac ctatatacat aataatataa ttttcgagtg aattgaccgc 2820

ccttcagcat cattatataa tgttatcgat ctaggtcttt gtgtgaaatt aaaagttatt 2880

tatacggtta gtacgatcgc gtaataacga aggtaaaaat atttcaggaa gagaagtggt 2940

tgggtatgaa atgccaaggc caaatattgg aatccacagg tttgtatttt tgctgtttaa 3000

gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc agagatcaat ttagtagtag 3060

aaaattttca gaagaaaatg aacttggctc accagttgct gctgttttct tcaattgtca 3120

gagggaaact gccgctagaa ggcgttga

Comparative Example 1: The nucleotide sequence of the coding portion of the self-arginine gene in which the substitution mutation occurred (SEQ ID NO: 5, sp-classic )

atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60

tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120

ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180

gatctcagat ccttcttcac actgatcatg atagatccag atgttcttgg tcctagtgat 240

ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300

tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360

tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420

agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480

gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga

{evaluation}

1. Comparison of number of leaves per stem of Sympodial

The finely grown tomato plants containing the nucleotide sequences of Examples 1 to 3 were found to have different numbers of leaves per stem of Sympodial plant. The difference in the number of leaves indicates that the time taken to harvest tomatoes is different. This suggests that the novel mutations disclosed in the present invention are important genetic resources for diversifying the cultivation period of tomato plants as the present invention is aimed at. (See Fig. 4)

2. Comparison of total number of flags

The fin-grown tomato plants containing the base sequences of Examples 1 to 3 showed an increase in the number of average flower buds as compared with Comparative Example 1. [ It can be inferred that as the number of flowers increases, the yield of tomatoes will also increase. In particular, in the case of the finely grown tomato plant containing the nucleotide sequence of Example 3, the number of flowers was 1.5 times higher than that of the conventional finely grown tomato plant. This means that the yields of the finely grown tomato plants containing the nucleotide sequences of Examples 1 to 3 disclosed in the present invention are advantageous from the viewpoint of the large graininess compared to the existing finely grown tomato plants. (See Fig. 5)

3. Overall evaluation

In view of the above, by utilizing the nucleotide sequences of Examples 1 to 3, it is possible to diversify the cultivation period of the finely grown tomato plants and to improve the cultivability. Therefore, the finely grown tomato plant containing the above base sequence can be cultivated economically, even in the plant cultivation, while preserving the labor-saving effect, which is an advantage of the existing finely grown tomato plant.

Genotype Mutation CC lines # sp-5732
(Example 1, SEQ ID NO: 1) Substitution 5732, 6257, 6258, 6259 sp-2857
(Example 2, SEQ ID NO: 2) deletion 871, 1679, 2798, 3249, 3441, 3480 sp-2798
(Example 3, SEQ ID NO: 3) deletion 873, 2857, 3186, 879, 2271, 3234 sp-classic
(Comparative Example 1) substitution

<110> Wonkwang University Center for Industry-Academy Cooperation <120> A Novel mutant tomato plants with modified determinate shoot          architecture <130> KL-P-01865 <160> 9 <170> KoPatentin 3.0 <210> 1 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 1 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gaagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 2 <211> 406 <212> DNA <213> Lycopersicon esculentum <400> 2 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 gatctcagat ccttcttcac actggattgt cacagacatt ccaggcacta cagattgctc 180 ttttggaaga gaagtggttg ggtatgaaat gccaaggcca aatattggaa tccacaggtt 240 tgtatttttg ctgtttaagc agaagaaaag gcaaacaata tcgagtgcac cagtgtccag 300 agatcaattt agtagtagaa aattttcaga agaaaatgaa cttggctcac cagttgctgc 360 tgttttcttc aattgtcaga gggaaactgc cgctagaagg cgttga 406 <210> 3 <211> 3148 <212> DNA <213> Lycopersicon esculentum <400> 3 acagttttct caatgatttt ttcttcttct tcatcttctc catcttctat tctcttcatc 60 ttcatagatt tagttacaga ttttcaataa ttcaacaacc atcaccatac tcacaattac 120 taccacctcc accatcacta tcaaccacta caatccttgc gatcaatctc tactacaaac 180 caatgaacca ttttcattat cataactagc acagctacta tcatcaacac atcatcaatt 240 accatatata ttcttcaccc atcgctgtta atatcactaa ctattaatat caatcaactt 300 caccaggaca atcaccatca ccactattaa atgtcatcat cacaccagtc attacaaaac 360 taacagtctc caacattacc agtaatcact aacaacaacc attattacaa acaatctcta 420 cttatttact tttattcaaa tatttattta gacaaaactg attttagtaa aacaaatgag 480 atcaatcttt ttctcgtgat taatttttaa gttggaatta gttccaaaat acatttaata 540 tagacaaata tgatactccc accgtcccat tttatgtaaa aaaacacgtc tcattttctt 600 atatggtaag tatttaaagg tataatttct cttttttacc tttattgatc ttaattttct 660 aatacattta tgagaagaga gaaaaatgag ttactttttt aaagaacgat ttgataaata 720 ttttaaaatc ttcattattt cttaaatttt gtgccagatc aaatgttgtc acataaaatg 780 agacggaaaa agtaaaacat atcaaacaca cccttaattt aaaatagtgt aggtactacc 840 taaaagtgga agttaattat ttgtttcccc aaaattaaat tttacccttg gacagcaatt 900 cctgtttaag ggttaattgt atagggacat acattttctt ctaatagtcc agggtagttt 960 ggttttcgat atgtggaaaa ttatctcgac ataaaatgct acagtaacta attagtacaa 1020 tatttagttt gtatttacca ttacatttag ctccacattc acataaattg gtagtacaac 1080 atttaatctt ctaatttgta ctataacatt ttcattaata aaaagtatgg tttctatctt 1140 caccattagc gtaacgttcg agtagagata tacatattta ttattataat atacgattgc 1200 aaatgccaaa gatggctaat tttgttttga gagactactg cattaagtaa attttttcag 1260 agacatgtat aagattaagt ctattgccaa ttctcaaata ttactctttt ttacttattg 1320 tggttattta tacatattaa gtgaactttc tttccttcct cagtaacttc taaacctagg 1380 gttgaagttc atggtggtga tctcagatcc ttcttcacac tggtatatat taatcttcaa 1440 cacttccaat ttactccgtc tgtctgtcct aatttatgtc acacattttc tatgatatat 1500 agttttagaa attattcaag accataactt tttaaagaaa aaatcataga ctttcttagt 1560 caacgtcaaa taaattgaga cggacaagat gacatgatta gtacatttat cttctattat 1620 tgacctctca ttttctttta tacattattt gacagatcat gatagatcca gatgttcctg 1680 gtcctagtga tccatatctc agggaacatc tacactggta tagacaacat atgccttaaa 1740 actaactcag tcaattttat cttcaattgt ttactttgga aggggaaatg acatgatcat 1800 tatatcatag tacaaattat tatgtaattt ctgttcgtct aaaaaatgtc actttagaaa 1860 aaactgataa tcatatacaa taccacaata aagatagaag aacatgtact aatattgaac 1920 ttaaataatg agtactagga gtattattaa ttaactttaa aaatgctagt caatatacct 1980 atgtttatat gttaaaaaat cctttatatt tggaaacatg agtactccta taccatacaa 2040 tgttgtcgta cagttgatta gacgggcaaa ttaaacaaat gtccaataat tgtactaatt 2100 aataactact tgttctcttc atctattatt agttattacc aaaaaaagag gactgcaaaa 2160 tggtgatatt attatgtgta acggaaaaaa acgtactcta tttaatatga tagaatcaaa 2220 gtgacatatt ttgttctagt tagacaaata agtaactgaa aagaggattt gaccatcttt 2280 acaggattgt cacagacatt ccaggcacta cagattgctc ttttggtatg tatccttaac 2340 ccataaatca aaataatgta ctttcttttt atttgccatt aatatctcta gtacaaaaaa 2400 gaaatattat aaaaaaaatt aatttcaatt tttatattat aggtttaaga taataatatt 2460 aaacgatatt ttagtctcta ccaaatagac gagcaaatta aaactaagaa agcactacat 2520 gttttcttta tattattagt ataaaaatat attataattt gcctggtggt aataggatca 2580 aagtattgat tcttaattat tattatataa ttaataataa tggtaaacaa aaagatataa 2640 agtgcttacc tcctaattcc ctatatgaaa aaatatactt acttaattac tctttttaca 2700 cgtaagcatg catttaaaaa aatattaaaa aattattcca gaggttatat ataatatgta 2760 tggataaaaa aaaaattcac ctatatacat aataatataa ttttcgagtg aattgaccgc 2820 ccttcagcat cattatataa tgttatcgat ctaggtcttt gtgtgaaatt aaaagttatt 2880 tatacggtta gtacgatcgc gtaataacga aggtaaaaat atttcaggaa gagaagtggt 2940 tgggtatgaa atgccaaggc caaatattgg aatccacagg tttgtatttt tgctgtttaa 3000 gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc agagatcaat ttagtagtag 3060 aaaattttca gaagaaaatg aacttggctc accagttgct gctgttttct tcaattgtca 3120 gagggaaact gccgctagaa ggcgttga 3148 <210> 4 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 4 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttctcgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 5 <211> 528 <212> DNA <213> Lycopersicon esculentum <400> 5 atggcttcca aaatgtgtga accccttgtg attggtagag tgattggtga agttgttgat 60 tatttctgtc caagtgttaa gatgtctgtt gtttataaca acaacaaaca tgtctataat 120 ggacatgaat tctttccttc ctcagtaact tctaaaccta gggttgaagt tcatggtggt 180 gatctcagat ccttcttcac actgatcatg atagatccag atgttcttgg tcctagtgat 240 ccatatctca gggaacatct acactggatt gtcacagaca ttccaggcac tacagattgc 300 tcttttggaa gagaagtggt tgggtatgaa atgccaaggc caaatattgg aatccacagg 360 tttgtatttt tgctgtttaa gcagaagaaa aggcaaacaa tatcgagtgc accagtgtcc 420 agagatcaat ttagtagtag aaaattttca gaagaaaatg aacttggctc accagttgct 480 gctgttttct tcaattgtca gagggaaact gccgctagaa ggcgttga 528 <210> 6 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 6 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly   1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr              20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser          35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser      50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Pro Gly Pro Ser Asp  65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly                  85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro             100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Lys         115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe     130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg                 165 170 175 <210> 7 <211> 154 <212> PRT <213> Lycopersicon esculentum <400> 7 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly   1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr              20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser          35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser      50 55 60 Phe Phe Thr Leu Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Cys Ser  65 70 75 80 Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro Arg Pro Asn Ile Gly                  85 90 95 Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln Lys Lys Arg Gln Thr             100 105 110 Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe Ser Ser Arg Lys Phe         115 120 125 Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala Val Phe Phe Asn     130 135 140 Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg 145 150 <210> 8 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 8 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly   1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr              20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser          35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser      50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Pro Gly Pro Ser Asp  65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly                  85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro             100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln         115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe     130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg                 165 170 175 <210> 9 <211> 175 <212> PRT <213> Lycopersicon esculentum <400> 9 Met Ala Ser Lys Met Cys Glu Pro Leu Val Ile Gly Arg Val Ile Gly   1 5 10 15 Glu Val Val Asp Tyr Phe Cys Pro Ser Val Lys Met Ser Val Val Tyr              20 25 30 Asn Asn Asn Lys His Val Tyr Asn Gly His Glu Phe Phe Pro Ser Ser          35 40 45 Val Thr Ser Lys Pro Arg Val Glu Val His Gly Gly Asp Leu Arg Ser      50 55 60 Phe Phe Thr Leu Ile Met Ile Asp Pro Asp Val Leu Gly Pro Ser Asp  65 70 75 80 Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly                  85 90 95 Thr Thr Asp Cys Ser Phe Gly Arg Glu Val Val Gly Tyr Glu Met Pro             100 105 110 Arg Pro Asn Ile Gly Ile His Arg Phe Val Phe Leu Leu Phe Lys Gln         115 120 125 Lys Lys Arg Gln Thr Ile Ser Ser Ala Pro Val Ser Arg Asp Gln Phe     130 135 140 Ser Ser Arg Lys Phe Ser Glu Glu Asn Glu Leu Gly Ser Pro Val Ala 145 150 155 160 Ala Val Phe Phe Asn Cys Gln Arg Glu Thr Ala Ala Arg Arg Arg                 165 170 175

Claims (9)

In genetically modified finely grown tomato plants,
Wherein the genetically modified finely grown tomato plant comprises a mutant self-mutation gene and the mutant self-mutation gene comprises the nucleotide sequence of SEQ ID NO: 1.
4. The genetically modified finely grown tomato plant according to claim 1, wherein the mutant self-mutation gene has the 382nd base of SEQ ID NO: 4 substituted by C in A.
The genetically modified finely grown tomato plant according to claim 1, wherein the mutant self-mutation gene encodes the polypeptide of SEQ ID NO: 6.
delete delete delete 4. The method according to any one of claims 1 to 3,
Wherein the genetically modified finely grown tomato plant is a pure genetically modified finely grown tomato plant.
4. The method according to any one of claims 1 to 3,
Wherein the genetically modified finely grown tomato plant is an inbreeding hybrid.
4. The method according to any one of claims 1 to 3,
Wherein the genetically modified finely grown tomato plant is a hybrid genetically modified finely grown tomato plant.

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