KR20180020761A - Composition for Enhancement of Immunity - Google Patents

Abstract

The present invention relates to a composition for immunity enhancement, and specifically, to a composition for immunity enhancement, which promotes the production of an antibody, increases the activity of immune cells, has no side effects and is safe for human body by comprising Canavalia gladiata and Arctium lappa, wherein the composition for immunity enhancement can be used in a health functional food or a pharmaceutical composition.

Description

[Composition for Enhancement of Immunity]

The present invention relates to a composition for improving immunity, and more particularly, to a composition for improving immunity comprising Canavalia gladiata ( beans ) and burdock ( Arctium lappa L. ) to promote the production of antibodies and increase the activity of immune cells, and is free from side effects and safe for human body.

Immunity is a self-defense system that exists in the body and is a process of recognizing, removing and metabolizing various substances or life forms that the human body invades from the outside as a foreign substance to oneself.

It may defend itself from damage by external stimuli or invasion of hospital microorganisms, but may damage its own tissues such as inflammatory reactions. Immune function to regulate the change to normal or to reduce the range of changes to suppress the immune function and enhance immunity is divided into. Hypersensitivity Immunosuppression means to inhibit an undesirably increased immune response, such as a response to an allergic autoantigen or a modified autoantigen caused by adverse reaction to an external agent.

Factors affecting immune function include genetic and environmental factors, age, sex, psychological stress, nutritional status, dietary habits, and disease.

To date, health functional foods having the function of regulating immune function are divided into functionalities for promoting decreased immune function, functionalities for controlling excessive immune function, and autoimmune function controlling functions for controlling immune responses to magnetic components.

These hypersensitivity reactions, such as asthma, seasonal or congenital rhinitis, allergic sinusitis, conjunctivitis, allergies due to food and medication, atopic dermatitis, allergies due to urticaria and bee venom .

Various immune enhancers and therapeutic agents are used in order to overcome the problems of the immunity function due to these various reasons. However, there are side effects problems and mainly commercial ones are used for therapeutic purposes rather than prevention. And a composition for improving immunity that is suitable for prevention is also desired.

The present inventors have completed the present invention by examining the synergistic effect of a mixture of soybean curd and burdock which has excellent immunity enhancing effect while screening natural foods so as to avoid side effects.

This product was funded by the Ministry of Food, Agriculture, Forestry, and Livestock and was supported by the Ministry of Agriculture, Forestry and Fisheries Technology Planning and Evaluation and supported by the High Value Added Food Technology Development Project (115036-3).

Related Prior Art: Korean Patent No. 0350911

              Korean Patent No. 0394584

It is an object of the present invention to provide a composition for immunity enhancement which is free from side effects and has no harmful effects on the human body even when taken for a long period of time, .

In order to achieve the above object, the present invention provides a composition for improving immunity comprising a mixture of soybean curd and burdock. In addition, in embodiments of the present invention, it may include its bud instead of the soybean curd.

The composition for improving immunity according to the present invention may include soybean curd and burdock as an extract, and these mixed extracts may be directly contained in the form of powder or solution.

According to one embodiment of the present invention, the mixed extract may be in the form of an organic solvent extract, a water extract, a supercritical extract, and a hot water extract of kojong and burdock.

Examples of the extraction solvent used for preparing the mixed extract of soybean curd and burdock include purified water; Lower alcohols having 1 to 10 carbon atoms such as methanol, ethanol, isopropyl alcohol and butanol; Polyhydric alcohols having 2 to 30 carbon atoms such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol; And hydrocarbon solvents having 1 to 30 carbon atoms such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform.

According to one embodiment of the present invention, the extracting solvent is preferably an extract obtained by extracting a 50 to 80% by volume of ethanol with respect to water in a solvent.

According to one embodiment of the present invention, the mixture of soybean curd and burdock is preferably mixed in a weight ratio of 1: 3 to 1: 5

The mixed extracts of soybean and burdock can be extracted and mixed, or the soybean and burdock raw materials can be mixed and extracted simultaneously by the above method.

According to an embodiment of the present invention, the composition for improving immunity may be used as a health functional food composition for prevention and / or treatment showing an immunological effect.

The composition for improving immunity according to the present invention may be contained in an amount of 0.0001 to 90% by weight based on the total weight of the health functional food composition.

According to one embodiment of the present invention, the composition for improving immunity can be used as a pharmaceutical composition for prevention and / or treatment showing an immunological effect.

According to one embodiment of the present invention, the pharmaceutical composition may contain 1-100 mg / kg body weight of the composition for enhancing immunity as an effective amount per day.

The composition for improving immunity of the present invention includes natural materials such as soybean curd and burdock, has no side effects, and can be used in food compositions without side effects even when taken for a long time. In addition, it promotes the production of antibodies and increases the activity of immune cells, thereby exerting an effect of enhancing immunity. In particular, the composition for improving immunity of the present invention can be used for the rapid recovery of immune function of a patient suffering from impaired immune function. In addition, even in the case of a semi-healthy person with impaired immune function or a normal person without immune function abnormality, The composition for improving immunity of the present invention can be used.

FIG. 1 shows the results of the measurement of changes in the number and activity of B cells in PEC (peritoneal leukocyte) isolated from the oral administration model of soybean curd extract.
FIG. 2 is a test result showing the activity of immune cells in a spleen isolated from an oral administration model of soybean curd extract.
FIG. 3 shows the results of measurement of the amount of reactive oxygen species (ROS) produced by extractive mixture treatment of koji: burdock in a mouse macrophage cell line (RAW264.7 cell).
FIG. 4 is a graph showing the results of measurement of the amount of ROS produced by a mixed treatment of koji: 70% koji extract of cormorant in mouse macrophage cell line (RAW 264.7 cell).
FIG. 5 shows the results of the blood IgA level change test by the ingestion of soybean curd and burdock complex in passivation stress-induced mice.
FIG. 6 is a graph showing the results of a change in white blood cell count (WBC) production by consumption of soybean and burdock complexes in a passive stress-induced mouse.
FIG. 7 shows the result of weight change test by consumption of soybean and burdock complex in passivation stress-induced mice.
FIG. 8 shows the results of measurement of changes in the amounts of IgA and IgG produced in the oral administration model of soybean curd and burdock extract.
FIG. 9 shows the result of the measurement of B cell change in peritoneal leached cells (PEC) isolated from the oral administration model of soybean curd and burdock extract.
FIG. 10 shows the results of the test for the activity of CD4 + CD69 + immune cells in a spleen isolated from a passive stress-induced mouse administered with soybean and burdock complexes.
FIG. 11 shows the results of test for the activity of CD8 + CD69 + immune cells in a spleen isolated from a passive stress-induced mouse administered with a combination of soybean curd and burdock.
FIG. 12 shows the results of the test for the activity of NK1.1 + CD69 + immune cells in spleens isolated from a passive stress-induced mouse administered with a soybean and soybean complex.
FIG. 13 shows the results of the test for the activity of CD4 + & CD8 + immune cells in the thymus isolated from the passive stress-induced mice administered with the soybean and soybean complex.
CG or J in the drawing is an abbreviation meaning soybean koji, and AL or W is an abbreviation meaning burdock.
IM_S in the drawing is an abbreviation for immobilization stress.

Hereinafter, the present invention will be described in more detail.

According to the present invention, jakdukong (Canavalia gladiata , and sword bean) and burdock ( Arctium lappa L. ).

Canavalia gladiata ) is a perennial vine plant belonging to the rosewood legume. It has no hairs on the stem. It has a length of 2 ~ 3 cm and a weight of 1.5 ~ 3 g. Leaves have long petiole and three small leaves. The flower of the soybean is light reddish purple or white, and a long flower stem grows from the axil of the leaf in the summer and runs with a gunshot. The end of the pod is curved or hooked, 20 ~ 30cm long, 5cm wide, and contains about 10 beans. The beans are about 3 cm long and have long seats on one side. The color of red beans is red or white, all of which can be used for food. Seeds, shells, leaves, stems and roots can be used as food materials. Soybean seeds contain the hydrolytic enzymes urease, hemagglutinin, canavalin, linolenic acid, saponin, choline, It contains various physiologically active substances such as lecithin, dietary fiber, and protein.

Soybean is called red bean in one room, red bean is used in pod, white bean is mainly used in white bean.

Burdock (Arctium lappa L. , Burdock) is 1.5 m high and the stem is purple and grows straight. Roots are thick and straight. Roots and seeds are used as medicinal herbs, and rootstocks are edible. The varieties have long roots, thick agar, good quality meat, and short roots.

The burdock root contains inulin and some palmitic acid, and contains arctin, arctigenin, isoarctigenin, archidic acid, alkaloid, and fatty oil. Especially, isochlorogenic acid is contained in large quantity and it is oxidized and discolored when it is left to slice.

The mixture of soybean curd and burdock may be contained in the form of an extract, and the mixed extract may be contained in the form of lyophilized powder or concentrated essence.

The mixture of soybean curd and burdock may be directly dried and pulverized and powdered. However, it may be included in the form of an extract in order to enhance the immunity enhancing effect and absorption rate, and the extract is preferably contained in a lyophilized or concentrated form .

According to one embodiment of the present invention, the mixed extract may be in the form of an organic solvent extract, a water extract, a supercritical extract, and a hot water extract.

Examples of the extraction solvent include purified water; Lower alcohols having 1 to 10 carbon atoms such as methanol, ethanol, isopropyl alcohol and butanol; Polyhydric alcohols having 2 to 30 carbon atoms such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol; And hydrocarbon solvents having 1 to 30 carbon atoms such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform.

The extract may be extracted by adding 4 to 10 times the extraction solvent to soybean curd and / or ground burdock. When the extraction solvent is water, it is preferably subjected to hot water extraction at 70 to 120 hours for 3 hours to 12 hours. When the extraction solvent is an organic solvent, it is preferable to perform cold extraction at 20 to 40 hours for 12 hours to 72 hours. Or when it is extracted with a mixed solvent of water and an organic solvent, it is preferable to carry out cold extraction.

The extraction solvent is preferably a solvent having 50 to 80% by volume of ethanol relative to water in order to extract the active ingredient better. The extract thus extracted may be lyophilized or evaporated using a reduced pressure concentrator to prepare a dry powder.

According to one embodiment of the present invention, the soybean curd (or an extract thereof) and the burdock (or an extract thereof) As a weight ratio of 1: 3 to 1: 5.

Mixing of the burdock to soybean showed a desirable synergistic effect, especially when included at the above mixing ratio, a synergistic effect on the enhancement of immunity.

The mixed extracts of soybean and burdock can be extracted and mixed, or the soybean and burdock raw materials can be mixed and extracted simultaneously by the above method. When the soybean and burdock raw materials are to be mixed and then extracted, it is preferable that the two raw materials are cut in the same or similar size or pulverized and mixed to be extracted.

According to one embodiment of the present invention, the composition for enhancing immunity further comprises at least one of a carrier, a diluent, an excipient and an additive, and is selected from the group consisting of tablets, pills, powders, granules, powders, capsules, It may be used in a form which is formulated as one.

Examples of the additive that can be further included in the present invention include natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors and the like), colorants, fillers (cheese, At least one component selected from the group consisting of fumaric acid and its salt, alginic acid and its salts, organic acid, protective colloid thickener, pH adjusting agent, stabilizer, preservative, antioxidant, glycerin, alcohol, .

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tautatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharine, aspartame, etc.) can be advantageously used as the flavor.

In addition to the above, the composition according to the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, Its salts, organic acids,

PH adjusters, stabilizers, antiseptics, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the composition according to the present invention may contain flesh for the production of natural fruit juices and vegetable drinks.

These components may be used independently or in combination.

Specific examples of the carrier, excipient, diluent and additives include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium phosphate, calcium Silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate And a mineral oil are preferably used.

When the composition according to the present invention is formulated, it is prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used.

According to an embodiment of the present invention, the composition for improving immunity may be used as a health functional food composition for prevention and / or treatment showing an immunological effect.

The composition for improving immunity according to the present invention may contain 0.0001 to 90% by weight, preferably 1 to 60% by weight, more preferably 30 to 50% by weight based on the total weight of the health functional food composition.

The foods to which the composition for improving immunity of the present invention can be added include various foods, powders, granules, tablets, capsules, syrups, beverages, gums, tea, vitamin complexes and health functional foods.

According to one embodiment of the present invention, the composition for improving immunity can be used as a pharmaceutical composition for prevention and / or treatment showing an immunological effect.

The content of the extract according to the present invention as an active ingredient in the pharmaceutical composition may be appropriately adjusted depending on the mode and purpose of use, the condition of the patient, the type of symptoms and the severity of the symptoms. According to one embodiment of the present invention, The composition for improving immunity may contain 1 to 100 mg / kg of body weight as an effective amount per day, but is not limited thereto.

The composition according to the present invention relates to the enhancement of immune function, and refers to a composition that contributes to enhancement of the immune function of the lowered human body, and may include the case of promoting normal immune function.

The biomarkers for confirming the function of enhancing immune function are NK cells, cytokines (IFN-α, IFN-β, TNF-α, IL-1, IL-6 and IL-12) , IL-5, IL-6, IL-9, IL-10 and IL-10 in the case of adaptive immunity. 13), percentage of lymphocyte population, and immunoglobulin. In addition, there are common cell survival rates (increased number of immune cells), splenocyte proliferation, and tissue weights (spleen, thymus) in congenital and adaptive immunity. The contents of each biomarker are as follows.

The cell survival rate means that the immune function increases as the number of immune cells increases.

Nitric oxide (NO) is produced by the action of the iNOS (Inducible Nitric Oxide Synthase) enzyme in macrophages, and the NO produced inhibits the degeneration of the predominant microorganism (pathogen) constituents and the action of enzymes containing Fe-S And exhibit antimicrobial action. NO is also secreted from activated macrophages or neutrophils, killing extracellular pathogens.

Inducible nitric oxide synthase (iNOS) oxidizes L-arginine to produce L-citrulline and NO, and iNOS is expressed by stimulation of cytokines in almost all cells.

COX2 (Cyclooygenase-2) is an enzyme that converts arachidonic acid into prostaglandin E2 (PGE2). It is expressed by inflammation or immunostimulation and is induced in response to various cytokine growth factors.

Phagocytosis, also called phagocytosis, is the action of macrophages such as white blood cells to remove bacteria or foreign matter. Macrophages have receptors for a variety of molecular substances, which when interacted with ligands and microorganisms Causes a reaction to increase the ability of the pathogen to pick up.

Cytokines are interleukin (IL), interferon (IFN), and tumor necrosis factors (TNF) that mediate intercellular signaling. IL-12, IL-15, IL-18, IFN-ν, and TNF-α, which are mainly produced by activated macrophages and lymphocytes, and the types of cytokines secreted from TH1-assisted T lymphocytes are IL-1, , Which are cytotoxic as inflammatory cytokines and are involved in cell mediated immune responses.

The lymphocyte population is divided into B lymphocyte, T lymphocyte, and NK cell, and the distinction between T lymphocyte and B lymphocyte and their subgroups is made by electron microscopy It is difficult to morphologically including the observation by the microscope. Their distinction is possible by using antibodies of the cell surface differentiation (CD) characteristic of each cell. CD3, CD56, CD16, CD94, and CD161, and B lymphocytes are CD10, CD19, CD20, CD21, CD22, CD23, CD24, CD40 and the like, and T lymphocytes are CD3 , Auxiliary T lymphocytes express CD4, cytotoxic T lymphocyte CD8 as a marker on the cell surface.

The spleen and thymus are tissues related to immune function. The spleen is a tissue involved in lymphocyte production, and the thymus is a tissue involved in T lymphocyte differentiation and maturation. Thus, the weight of spleen thymus tissue is measured to see the systemic effects of immune-related tissues.

The spleen is a tissue that makes lymphocytes and destroys aging red blood cells. This means that the immune function is improved as the number of splenocytes isolated from an experimental animal ingested the test substance increases.

Immunoglobulin (Ig) is a serum protein that plays a major role in humoral immunity. IgA, IgD, IgE, IgG and IgM are classified into IgA, IgG and IgM. Immunoglobulin, IgA accounts for about 20% of immunoglobulins in serum and is present in mucus, such as saliva and secretion fluid, and is involved in defense of mucous membrane infection. IgG is divided into four subgroups IgG1, IgG2, IgG3, and IgG4, and participates in neutralization, sedimentation, aggregation reaction of toxin or virus. IgM has a beam-tightening synthesis and high coagulation reactivity. Increased immunoglobulin (IgA, IgG, IgM) means increased immune function.

Complement is a protein contained in blood and lymph. The complement does not have immunological specificity and binds to the complex of antigen and antibody, and acts as hemolysis and lytic action to remove bacteria and antigens that have passed the physical barrier. Complement consists of more than 20 proteins, mainly C3, C4, C5. Activation of complement is associated with a variety of immune responses such as activation of macrophages and lymphocytes and enhancement of immune function.

NK cells are white blood cells in the blood that are responsible for innate immunity. These cells recognize virus-infected cells or stressed cells and directly kill or secrete inflammatory cytokines. Changes in NK cell activity can be used to determine whether the immune function is enhanced.

Hereinafter, the present invention will be described in more detail with reference to examples and comparative examples. It should be understood, however, that the present invention is not limited to the above-described embodiments, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It is to be understood that equivalents and modifications are possible.

Example : Preparation of Soybean and Burdock Extracts

Soybean koji and burdock were mixed with ethanol and purified water according to the standards of the Korean Pharmacopoeia, and the mixture was cooled to room temperature (20 ± 5) for 5 days by adding about 4 to 10 times of 30% and 70% alcohol. Soybean and burdock were pulverized and added with about 4 to 10 times of purified water, followed by cooling at room temperature (20 ± 5) for 1 day. The extracted extracts were filtered using Wattman # 3 filter paper, and the extracted extracts were completely evaporated at 45 using a vacuum concentrator to obtain dried extracts.

Experimental Example  1: Isolate from soybean extract extract Of PEC (peritoneal leached cells)  B cell change measurement

PEC (peritoneal leukocyte) was extracted from a mouse model of oral extract of koji, a 30% ethanol extract and a 70% ethanol extract, and then fluorescent stained with B220 (B cell marker) and CD69 (activation marker) Respectively. As a result, it was confirmed that the number and activity of B cells were increased most in 70%

The results are shown in Fig.

Experimental Example  2: Identification of immune cell activity in spleen isolated from oral extract of soybean curd extract

The spleen, which is a major immunoreactor, was isolated from the mouse, which was orally administered with 30% ethanol extract and 70% ethanol extract, and the cells were separated, and then CD4 (CD4 T cell marker), CD8 (CD8 T cell marker) , NK1.1 (NK cell marker) and CD11b + F4 / 80 (macrophage marker) antibodies and analyzed by flow cytometry. As a result, it was confirmed that the number and activity of CD4, CD8 T cell and macrophage were most increased in the spleen of mouse group administered with 70% extract of koji malt extract.

The results are shown in Fig.

Experimental Example  3: Mouse macrophage cell line ( RAW264 .7 cells) in soybean: burdock extract mixture treatment ROS  Production amount measurement

To determine the reduction of ROS production by the mixing ratio of soybean and burdock extracts, 70% kojong extract was fixed at 100 μg / ml in RAW264.7 cell line and the ratio of soybean and burdock extracts was 1: 4, 1: 2 , 30% and 70% ethanol extracts were mixed with 1: 1, 2: 1 and 4: 1 rum extracts. When LPS (500 ng / ml), which is capable of promoting the production of ROS, was treated 12 hours before the measurement of ROS, the amount of ROS increased by LPS was mixed with the koji and burdock 70% alcohol extract at a ratio of 1: 4 , The highest decrease was observed

The results are shown in Fig.

Experimental Example  4: mouse macrophage line ( RAW264 .7 cells): koji: 70% of burdock by percentage ration of alcohol extract ROS  Production amount measurement

In order to determine the optimal concentration of kojong and burdock extracts, the ratio of kojong and burdock extracts was 1: 4, 1: 2, 1: 1 , And 2, 1, 4: 1, 5: 1, 6: 1, 7: 1 and 8: 1, and the treatment of burdock extract alone, 25, 50 and 100 ㎍ / : 4, the most decrease in ROS was observed.

The results are shown in Fig.

Experimental Example  5: dissimilation  In the stress-induced mice, IgA  Level change test

70% koji extract (CG70% E), 70% burdock extract (AL70% E) and 70% soybean koji were added to the mouse, which caused the stress to be disturbed for 2 days each day for 2 hours in a narrow frame. (CG + AL, 1: 4) mixed with 1: 4 ratio of extracts and 70% of burdock extracts were orally administered to C57BL / 6 at 8 weeks of passivation stress. As a result of measuring IgA levels in serum, 70% koji extract (CG70% E) 100 mg / kg (p <0.05) and 70% koji extract were mixed with 1: 4 ratio of 70% IgA levels were significantly increased in the 100 mg / kg (p <0.01) and 50 mg / kg (p <0.05) groups compared with the passive control group (IM_S-CTL) appear.

In particular, the highest IgA level was observed in the combination of 70% kojong extract and 70% burdock extract in a ratio of 1: 4.

The results are shown in Fig.

Experimental Example  6: dissimilation  Changes in Leucocyte Count (WBC) Production by Intake of Soybean and Burdock Complex in Stress-Induced Mice

(CG + AL, 1: 4) mixture of 70% koji extract (CG70% E), 70% burdock extract (AL70% E), 70% koji extract and 70% 4) was orally administered to stress-induced 8 week old C57BL / 6 by immobilization for 21 days for 2 hours each day. The number of WBCs in the serum was significantly higher than that of the immortalized control (IM_S-CTL). In particular, the number of white blood cells (WBC) (CG + AL, 1: 4) 100 mg / kg (p <0.01), 50 (mg / kg, p <0.001) and 70% of the soybean extract and the 70% mg / kg (p <0.05) showed an increase in the number of white blood cells (WBC) statistically significant compared with the immobilized control group (IM_S-CTL).

In particular, the combination of 70% kojong extract and 70% burdock extract at a ratio of 1: 4 showed the highest increase in WBC counts.

The results are shown in Fig.

Experimental Example  7: dissimilation  Weight change test by ingestion of soybean and burdock complex in stress-induced mice

(CG + AL, 1: 4) mixture of 70% koji extract (CG70% E), 70% burdock extract (AL70% E), 70% koji extract and 70% 4) was orally administered to stress-induced 8 week old C57BL / 6 by immobilization for 21 days for 2 hours each day. (70%) of alcohol extract (CG70% E) 100 mg / kg, 70% of burdock extract (AL70% E) and 100% of body weight of immature control group (IM_S-CTL) (CG + AL, 1: 4) 100 mg / kg of the mixture of 1: 4 ratio of the extract of cormorant and 70% of the koji extract to the weight of the control group (IM_S-CTL) Decrease.

In particular, the lowest weight loss was observed in the combination of 70% koji extract with 70% koji extract and 1: 4 ratio.

The results are shown in Fig.

Experimental Example  8: Oral administration of soybean and burdock extract IgA  And IgG  Production change measurement test

A sample (JW) of 70% koji extract (J), 70% burdock extract (W), 70% koji extract and 70% koji extract of burdock were mixed at a ratio of 1: 4 to 8-week-old C57BL / 6 After daily oral administration for weeks, mice were sacrificed and the amount of IgA and IgG produced was measured in the extracted blood. As a result, it was confirmed that the IgA and IgG production in mouse group treated with the mixture of soybean koji and burdock at the ratio of 1: 4 was the greatest increase compared to the control group.

The results are shown in Fig.

Experimental Example  9: Measurement of B cell change in peritoneal leached cells (PEC) isolated from oral administration model of soybean curd and burdock extract

Peritoneal leukocyte (PEC) was extracted from the mouse model of oral administration of soybean curd and burdock extract and fluorescent stained with B220 (B cell marker) and CD69 (activation marker) antibody and analyzed by flow cytometry. As a result, And the number and activity of B cells were increased most in mouse group treated with burdock.

The results are shown in Fig.

Experimental Example  10: Administration of soybean and burdock complex dissimilation  Test for the activation of CD4 + CD69 + immune cells in spleens isolated from stress-induced mice

The number of CD4 + CD69 + T cells was decreased in spleen-treated mice compared to normal mice. The number of CD4 + CD69 + T-activated cells was decreased in 100, 50 mg / kg of soybean curd extract, burdock extract, The CD4 + CD69 + T cell activity was significantly increased compared to the control group.

In particular, the highest immunocellular activity was observed in the combination of soybean extract and burdock extract at a ratio of 1: 4.

The results are shown in Fig.

Experimental Example  11: Administration of soybean and burdock complex dissimilation  Tests for activation of CD8 + CD69 + immune cells in spleens isolated from stress-induced mice

In Spleen, the number of CD8 + CD69 + T active cells decreased in the passive control group compared with the normal mice. In the group of 100, 50 mg / kg of soybean extract, Burdock extract, The CD8 + CD69 + T cell activity was significantly increased compared to the control group.

In particular, the highest immunocellular activity was observed in the combination of soybean extract and burdock extract at a ratio of 1: 4.

The results are shown in Fig.

Experimental Example  12: Administration of soybean and burdock complex dissimilation  Activation test of NK1.1 + CD69 + immune cells in spleen isolated from stress-induced mice

In Spleen, NK1.1 + CD69 + NK cells were decreased in the immobilized control group compared with normal mice, and 100, 50 and 25% of the soybean koji extract, burdock extract and 1: 4 mixture of soybean koji mg / kg, NK1.1 + CD69 + NK cell activity was significantly increased in a concentration-dependent manner compared with the control group.

In particular, the highest immunocellular activity was observed in the combination of soybean extract and burdock extract at a ratio of 1: 4.

The results are shown in Fig.

Experimental Example  13: Administration of soybean and burdock complex dissimilation  Test for the activation of CD4 + & CD8 + immune cells in thymus isolated from stress-induced mice

In the thymus, CD4 + & CD8 + activated cells were decreased in the passive control group compared to the normal mice, and 100, 50 and 25 mg / kg of the soybean koji extract, Burdock extract, , CD4 + & CD8 + cell differentiation was significantly increased in a concentration-dependent manner compared with the control group.

In particular, the highest immunocellular activity was observed in the combination of soybean extract and burdock extract at a ratio of 1: 4.

The results are shown in Fig.

Formulation examples of health functional foods and pharmaceutical compositions comprising the composition for improving immunity according to the present invention will be described, but the present invention is not limited thereto.

[ Manufacturing example  1: Composition for enhancing immunity]

According to Example 1, 70% of the extracts of soybean curd and burdock were respectively prepared, dried and pulverized to prepare powders. The prepared powder was mixed with soybean curd and burdock at a weight ratio of 1: 4 to prepare a composition for improving immunity.

[Food for immunity promotion]

(One) Wire  Produce

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and then the mixture was prepared into powder having a particle size of 60 mesh by a pulverizer. Black beans, black sesame seeds and perilla seeds were each steamed and dried by known methods, and then power distribution and pulverization were carried out to prepare powder having a particle size of 60 mesh. Thereafter, 30% by weight of brown rice, 15% by weight of yulmu, 20% by weight of barley, 9% by weight of glutinous rice, 7% by weight of perilla seeds, 8% by weight of black soybeans, 7% by weight of black sesame seeds, %, 0.5% by weight of manure, and 0.5% by weight of sulfur.

(2) Chewing gum  Produce

Chewing gum was prepared by mixing 20% by weight of a gum base, 76.9% by weight of sugar, 1% by weight of a flavor, 2% by weight of water and 0.1% by weight of a composition for improving immunity prepared in Preparation Example 1.

(3) Health drinks manufacturing

0.0001 wt.% Of niacinamide, 0.0001 wt.% Of sodium riboflavin, 0.0001 wt.% Of pyridoxine hydrochloride, 0.001 wt.% Of inositol, 0.002 wt.% Of orthoacetic acid, 98.7362 wt.% Of water and 0.2 wt. 1% by weight of the composition for improving immunity, which was prepared in Example 1, was blended to prepare a health drink by a conventional method.

(4) health supplement manufacturing

A mixture of 55% by weight of spirulina, 10% by weight of guar gum enzyme hydrolyzate, 0.01% by weight of vitamin B1 hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, And 10% by weight of the composition for enhancing immunity prepared in Preparation Example 1 were mixed to prepare a refillable health supplement food by a conventional method.

(5) Manufacture of alcoholic beverages

0.5% by weight of the composition for enhancing immunity prepared in Preparation Example 1 was dissolved in a mixture of soju, beer,

Mixed with fruit wine to make an emulsion state, and mixed at 9,000 rpm with a high-speed mixer to prepare a liquor.

[Health Functional Food or Pharmaceutical Composition for Enhancing and Treating Immunity]

(One) Powder  Produce

2 g of the composition for enhancing immunity prepared in Preparation Example 1 was mixed with 1 g of lactose and filled in an airtight container to prepare a powder.

(2) Preparation of tablets

100 mg of the immunomodulating composition prepared in Preparation Example 1, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and tableted by a conventional tableting method.

(3) Capsule  Produce

100 mg of the immunomodulating composition prepared in Preparation Example 1, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and filled in gelatin capsules to prepare capsules.

CG or J in the drawing is an abbreviation meaning soybean koji, and AL or W is an abbreviation meaning burdock.
IM_S in the drawing is an abbreviation for immobilization stress.

Claims (11)

Canavalia gladiata , 刀豆) and burdock ( Arctium lappa L. ). &lt; / RTI &gt; [Claim 2] The composition for immunomodulation according to claim 1, wherein the soybean and burdock mixture is a dry or crushed powder or an extract. [Claim 3] The composition according to claim 2, wherein the extract is an organic solvent extract, a water extract, a supercritical extract, or a hot water extract. 4. The method according to claim 3, wherein the extraction solvent of the extract is purified water; Lower alcohols having 1 to 10 carbon atoms; Polyhydric alcohols having 2 to 30 carbon atoms; And a hydrocarbon-based solvent having 1 to 30 carbon atoms. [Claim 4] The composition for immunity enhancement according to claim 3, wherein the extraction solvent of the extract is a solvent having 50 to 80% by volume of ethanol relative to water. [Claim 2] The composition for immunity enhancement according to claim 1, wherein the mixture of soybean curd and burdock is a mixture of soybean curd and burdock at a ratio of 1: 3 to 1: 5. [Claim 3] The composition for enhancing immunity according to claim 2, wherein the extract is a mixed extract obtained by mixing extracts of soybean curd or burdock, or a mixed extract of soybean curd and burdock at the same time after mixing. 7. The immunostimulating food comprising the immunoconjugate composition according to any one of claims 1 to 7. The immunostimulating food according to claim 8, wherein the composition for enhancing immunity contains 0.0001 to 90% by weight based on 100% by weight of the food composition. A pharmaceutical composition for immunostimulation comprising the immunoconjugate composition according to any one of claims 1 to 7. [Claim 11] The pharmaceutical composition according to claim 10, wherein the composition for enhancing immunity contains 1 to 100 mg / kg body weight as an effective amount per day in the immunoenhancing pharmaceutical composition.

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