KR20180005912A - Manufacturing method of eco-friendly amino acid fertilizer from the slaughtered livestock blood - Google Patents

Abstract

The present invention relates to a method of manufacturing an eco-friendly amino acid fertilizer by using the blood of slaughtered livestock. More specifically, the method includes: a slaughtered livestock blood heating step of heating the blood of slaughtered livestock; a slaughtered livestock blood smashing step of smashing the blood heated through the heating step; a purifying step of purifying the blood smashed through the slaughtered livestock blood smashing step; a decomposing step of decomposing the blood purified through the purifying step by mixing proteolytic enzymes with the blood; a microorganism fermenting step of adding microorganisms to the blood decomposed through the decomposing step to ferment the blood; and a fertilizer component mixing step of mixing the fermented matter with water, nitrogen, phosphorus, potassium, and a mineral mixture. As such, the eco-friendly amino acid fertilizer manufacturing method is capable of providing an eco-friendly amino acid fertilizer without chemical components, having outstanding performance by using the blood of slaughtered livestock.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing an amino acid-free amino acid fertilizer using a slaughter blood,

The present invention relates to a method for producing an environmentally friendly amino acid fertilizer using slaughter blood, and more particularly, to a method for producing an environmentally friendly amino acid fertilizer which is excellent in efficacy using a disposal slaughtered blood, will be.

The types and specifications of fertilizers are determined by the 'Fertilizer Process Specification' notified by the Ministry of Agriculture and Forestry in Korea. The cost of foliar application, which is included in Type 4 compound fertilizer, for nutrient solution cultivation or gardens, Has been developed in the form of solid and liquid fertilizer according to the permitting standards of ordinary fertilizers specified by the fertilizer process standard. The amino acid fermented PFD belonging to the byproduct fertilizer was also developed as a liquid fertilizer according to the 'Fertilizer Process Standard'. However, most of the liquid fertilizers other than the amino acid fermentation PFD are produced by mixing with inorganic compounds such as nitrogen, phosphoric acid, garlic and boric acid, magnesium, manganese, iron, or trace elements And there is no liquid fertilizer manufactured by using directly high-protein raw blood material or protein decomposition product obtained by degrading blood.

On the other hand, protein is an important nitrogen source that supplies various essential and non essential amino acids necessary for growth and metabolism of animals, plants, microorganisms, etc., and supplies calories with carbohydrates and fats. In this respect, proteins, for example, proteins of soybean origin, casein of milk origin, animal proteins such as gelatin are undissolved, or hydrolyzed by acids or enzymes, and they are widely used as food or microbial media.

Blood produced after slaughter has already been used as feed and partly as a nutrient for plants and used as a soil inoculum.

However, conventionally, the technology of using blood as a plant nutrition source is mainly manufactured by a chemical process, so that only the corresponding ingredient is supplied to the plant, and the ingredients that are not absorbed into the growth of the crop are left in the land as they are, In addition, the nutrients absorbed by the crop are still in short supply, and the components necessary for growing the crops and the components remaining in the soil excessively can not be accurately known, the series can not be formed and the nutrients can be supplied unevenly There has been a problem that it can hinder the cropping of crops.

Korean Patent Registration No. 10-1331253 (November 13, 2013). Korean Patent Registration No. 10-1246342 (March 23, 2013).

It is an object of the present invention to provide a method for producing an amino acid fertilizer which is excellent in efficacy using a slaughter blood to be disposed of and does not contain chemical components and is environmentally friendly.

In one embodiment, the content of the disclosure includes a slaughter blood heating step for heating slaughter blood, a slaughter blood slaughter step for slaughtering slaughter blood heated through the slaughter blood heating step, a slaughter blood slaughter step A step of purifying the blood, a step of mixing and decomposing the proteolytic enzyme into the slaughter blood purified through the purification step, a step of fermenting the microorganism by adding the microorganism to the slaughter blood digested through the step of decomposing, And a fertilizer component mixing step of mixing water, nitrogen, phosphorus, potassium, and a mineral mixture into the fermented product fermented through the fermentation step, and a method for producing an environmentally friendly amino acid fertilizer using the slaughter blood.

Preferably, the digestion step comprises mixing and dissolving 0.5 to 1.5 parts by weight of proteolytic enzyme in 100 parts by weight of slaughtered blood purified through the purification step.

More preferably, the decomposition step is carried out at a temperature of 60 to 70 DEG C for 8 to 10 hours.

More preferably, the microbial fermentation step comprises mixing 10 to 20 parts by weight of the microorganism and 10 to 20 parts by weight of the additive in 100 parts by weight of the slaughtered blood decomposed through the decomposition step, and heating the mixture at 30 to 35 DEG C for 2 to 3 days Wherein the microorganism comprises lactobacillus plantarum CU03 KACC 91103, wherein the additive comprises 100 parts by weight of molasses, 0.25 to 0.35 parts by weight of salt, 0.05 to 0.1 parts by weight of brown sugar, 0.05 to 0.1 parts by weight of kiwifruit .

The method for producing an environmentally friendly amino acid fertilizer using slaughter blood as described above is excellent in efficacy using slaughtered blood to be disposed of, and exhibits an excellent effect of providing an environmentally friendly amino acid fertilizer because chemical components are not contained.

In addition, since the process of fermenting slaughter blood into microorganisms proceeds, an animal amino acid having a high absorption rate into plants is a main component, and thus an excellent effect of providing an amino acid fertilizer that does not contaminate the soil due to a small amount of components remaining in the soil is exhibited.

1 is a flowchart illustrating a method for manufacturing an environmentally friendly amino acid fertilizer using slaughter blood according to an embodiment of the present invention.
2 is a flowchart illustrating a method for manufacturing an environmentally-friendly amino acid fertilizer using slaughter blood according to another embodiment.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

A method for producing an environmentally friendly amino acid fertilizer using the disclosed slaughter blood includes a slaughter blood heating step (S101) for heating slaughter blood, a slaughter blood slaughter step (S103) for slaughtering slaughter blood heated through the slaughter blood heating step (S101) (S105) for purifying the slaughtered blood through the slaughter blood crushing step (S103); a decomposition step (S107) for mixing and decomposing the proteolytic enzyme in the purified slaughter blood through the purification step (S105) The fermentation step S109 of fermenting the microorganism by adding the microorganism to the slaughter blood decomposed through the decomposition step S107 and the step S109 of fermenting the microorganism fermentation step S109 to add water, And a fertilizer component mixing step (S111).

The heating step (S101) of heating the slaughter blood comprises heating the slaughter blood at a temperature of 100 to 120 DEG C for 10 to 30 minutes to remove various bacteria remaining in the slaughter blood, It is a step to improve the efficiency of solidification.

If the heating temperature is less than 100 ° C or the heating time is less than 10 minutes, the above effect is insignificant. If the heating temperature exceeds 120 ° C or the heating time exceeds 30 minutes, the above- Thereby increasing the processing time and manufacturing cost.

The slaughter blood (S 103) is a step of pulverizing slaughtered blood heated through the slaughter blood heating step (S 101). The slaughter blood heated in the slaughter blood heating step (S 101) is introduced into an inhaler And then pulverized to a size of 0.01 to 0.05 mesh using a pulverizer after it is suctioned and transferred into the pulverizer.

As the slaughtered blood crushed through the slaughter blood crushing step (S103) is finely crushed, the efficiency of the fermentation process proceeding through the microbial fermentation step (S109) is improved.

If the particle size of the pulverized slaughter blood is less than 0.01 mesh, it is necessary to use a separate pulverizing apparatus, so that the efficiency of the manufacturing process is lowered. If the particle size of the pulverized slaughter blood exceeds 0.05 mesh The efficiency of the microbial fermentation step (S109) is lowered.

At this time, the pulverizer is provided with an inhaler for sucking slaughter blood, and is not particularly limited as long as it can pulverize slaughter blood with the mesh size described above, and any of them can be used.

The purifying step (S105) is a step of purifying the slaughtered blood through the slaughter blood crushing step (S103). The slaughter blood is crushed through the slaughter blood crushing step (S103) It is a step to remove contained impurities.

At this time, the filtration device is not particularly limited, and any filtration device may be used as long as it can remove impurities contained in the pulverized slaughter blood, but it is preferably made of a filter press.

The digesting step (S107) is a step of mixing and decomposing the proteolytic enzyme into the slaughter blood purified through the purifying step (S105). In 100 parts by weight of the slaughter blood purified through the purifying step (S105) and 0.5 to 1.5 parts by weight of a protease are mixed and decomposed at a temperature of 60 to 70 DEG C for 8 to 10 hours.

If the content of the protease is less than 0.5 part by weight, the decomposition temperature is less than 60 占 폚 or the decomposition time is less than 8 hours, the decomposition step (S107) The fermentation efficiency of the slaughter blood is lowered in the microorganism fermentation step (S109). When the content of the protease exceeds 1.5 parts by weight, or when the decomposition temperature exceeds 70 deg. C or the decomposition time exceeds 10 hours, The efficiency of the degradation process of the protein components contained in the protein is not greatly improved but the production cost is increased.

At this time, it is preferable to use an alkaline protease showing the alkalinity of the proteolytic enzyme. If the alkaline protease is used as described above, the pH of the produced fertilizer shows alkalinity, It is possible to suppress the degradation to acidity.

The microorganism fermentation step (S109) is a step of adding microorganisms to the slaughter blood decomposed through the decomposition step (S107) and fermenting the microorganisms. 10 to 20 parts of microorganisms are added to 100 parts by weight of the slaughter blood decomposed through the decomposition step (S107) And 10 to 20 parts by weight of the additive are mixed at a temperature of 30 to 35 DEG C for 2 to 3 days.

In this case, lactobacillus plantarum CU03 KACC 91103 is used as the microorganism. After isolating the strain from an environmental cleaning agent (IFPIX), the strain is cultured using a Lactobacillus selective medium, followed by Gram stain and MRS culture The strain can be selected primarily by culturing it through cultivation, and these strains can be identified with Lactobacillus plantarum CU03 using an API kit.

In addition, the Lactobacillus plantarum CU03 KACC 91103 exhibits acid resistance in the pH range of 2.5 to 7, and has the ability to produce the biliary and lactic acid capable of growing under the condition of oxgall 0.1% (w / v) It has the property of inhibiting the number of microorganisms involved in the odor generation process that occurs during the decay of feces and significantly lowering the amount of ammonia which is the main component of odor generation. It also has antioxidative activity and IgM activity Increase.

The additive is composed of molasses, salt, brown sugar, and kiwi juice, wherein 0.25 to 0.35 parts by weight of salt, 0.05 to 0.1 parts by weight of salt, and 0.05 to 0.1 part by weight of kiwi juice are mixed with 100 parts by weight of molasses.

The molasses may be a byproduct when making sucrose, or honey, which is produced when refining sugar or making ice crystals. Molasses is a pale yellow transparent and viscous sugar solution, and the glacial molasses contains water, protein, carbohydrates and minerals. It is also known as molasses, refined molasses, and molasses. It is also used as a raw material for confectionery and jam, and to be eaten on hot cakes, etc., and sometimes used as a raw material for industrial alcohol.

When the content of the molasses is less than 100 parts by weight, the amount thereof is too small and the effect of improving the fermentation efficiency in the microbial fermentation step is insignificant. When the molasses content exceeds 100 parts by weight, The fermentation by the fermentation process can be excessively advanced.

Also, the salt contains 0.25-0.35 wt%, and plays a role in controlling pests and controlling the growth of weeds and increasing the sugar content and storage stability of plants. The salt contained about 45% of chlorine (Cl), 30.6% of sodium (Na), 7.7% of sulfur (S), and other trace elements such as magnesium (Mg), calcium (Ca) It is an element that supplies trace elements that are insufficient in the soil to further improve plant growth.

At this time, if the content of the salt is less than 0.25 parts by weight, the effects described above are insignificant. If the content of the salt exceeds 0.35 parts by weight, the growth of the plant may be inhibited.

The brown sugar is contained in an amount of 0.05 to 0.1 part by weight and 0.05 to 0.1 part by weight of the kiwi juice. The brown sugar and the kiwi juice are mixed at a weight ratio of 1: 1, and then the fermentation is performed for 10 to 20 days. The protease contained in the kiwi juice is able to decompose the protein contained in the slaughter blood, and thus the efficiency of the fermentation step of the microorganism is further improved do.

The fermentation process of the kiwi juice is described in more detail. The fermentation process of the kiwi juice is carried out by kneading 3 to 4 pieces of kiwi, and then mixing the mixture with the brown sugar at a ratio of 10: 3 by weight to the fermentation vessel such as a jar. The mixture of kiwi and brown sugar put into the container is covered so as not to be exposed to air and fermented for 10 to 20 days at a temperature of 5 to 15 ° C in a shaded place where it is not sunny, . ≪ / RTI >

Also, in the fermentation step (S109), the lactobacillus planta CU03 KACC 91103, which is a microorganism, is fermented, and excreted after digesting the fungi and the bacteria with food, contains a large amount of animal amino acids. It is rapidly absorbed by plants and plays a role in increasing plant growth rate.

If the fermentation temperature is less than 30 ° C., the fermentation rate may be slow or the fermentation effect may be insignificant. If the fermentation temperature is more than 35 ° C., the fermentation process may progress rapidly and the fermentation may proceed excessively.

The fertilizer component mixing step (S111) is a step of mixing water, nitrogen, phosphorus, potassium, and a mineral mixture into the fermented product fermented through the microbial fermentation step (S109) 15 to 25 parts by weight of water, 5 to 7 parts by weight of nitrogen, 1 to 3 parts by weight of phosphorus, 1 to 3 parts by weight of potassium and 0.01 to 0.1 part by weight of a mineral mixture are mixed with 100 parts by weight of the fermented product, 6.5 to 7.0.

At this time, the water serves to dilute the fermented product through the microbial fermentation step (S109) to produce a liquid form.

The mineral mixture is composed of boric acid and molybdenum. The mineral mixture comprises 100 parts by weight of boric acid and 2 to 10 parts by weight of molybdenum. .

The above boric acid and molybdenum are the trace elements required for the crop to which the fertilizer is applied. If the mineral mixture composed of boric acid and molybdenum is contained as described above, the fertilizer containing the trace element is produced, and there is no need to separately dispense the trace element fertilizer Thereby providing a compound fertilizer.

If the content of the mineral mixture is less than 0.01 part by weight, the feeding efficiency of the trace elements to the crops to be cultivated decreases. If the content of the mineral mixture exceeds 0.1 parts by weight, the crops to be cultivated are overproduced , It is not preferable from the viewpoint of manufacturing cost.

Also, between the heating step (S101) of slaughter blood and the step (S103) of slaughtering blood, a water separation step (S102) for water separation of heated slaughter blood through the centrifugal heating step (S101) The water separation step S102 through the centrifugal separator as described above removes the moisture contained in the slaughter blood, thereby further improving the efficiency of the fermentation process in the microbial fermentation step (S109).

At this time, the centrifugal separator is not particularly limited as long as it can remove moisture contained in slaughter blood, and any centrifuge may be used.

The preparation of the environmentally friendly amino acid fertilizer using the disclosed slaughter blood is completed after the fertilizer component mixing step (S111) is completed. The prepared environmentally friendly amino acid fertilizer is put into an individual container using a quantitative injector, .

Therefore, the method of producing environmentally friendly amino acid fertilizer using slaughter blood as described above is excellent in efficacy using slaughtered blood to be disposed of, is environmentally friendly since chemical components are not contained, and also a process of fermenting slaughter blood into microorganisms An amino acid fertilizer that does not contaminate the soil due to a small amount of components remaining in the soil can be provided.

S101; Slaughter blood heating step
S102; Oil-water separation step
S103; Slaughter blood crushing step
S105; Purification step
S107; Decomposition step
S109; Microbial fermentation step
S111; Fertilizer component mixing step

Claims (8)

A slaughter blood heating step of heating the slaughter blood;
A slaughter blood crushing step of crushing slaughtered blood heated through the slaughter blood heating step;
A purification step of purifying the slaughtered blood pulverized through the slaughter blood purification step;
A digestion step of mixing and decomposing the proteolytic enzyme in the slaughter blood purified through the purification step;
A microbial fermentation step in which microbes are added to the slaughtered blood which has been degraded through the decomposition step and fermented; And
And a fertilizer component mixing step of mixing water, nitrogen, phosphorus, potassium, and a mineral mixture into the fermented product fermented through the microbial fermentation step.
The method according to claim 1,
Wherein the step of heating the slaughter blood and the step of heating the slaughter blood are performed between the step of heating the slaughter blood and the step of slaughtering the blood, Gt;
The method according to claim 1,
Wherein the slaughter blood is heated at a temperature of 100 to 120 ° C for 10 to 30 minutes.
The method according to claim 1,
Wherein the digesting step comprises mixing and dissolving 0.5 to 1.5 parts by weight of a protease in 100 parts by weight of purified slaughter blood through the purification step.
The method according to claim 1 or 4,
Wherein the decomposing step is performed at a temperature of 60 to 70 DEG C for 8 to 10 hours.
The method according to claim 1,
Wherein the microbial fermentation step comprises mixing 10 to 20 parts by weight of the microorganism and 10 to 20 parts by weight of the additive in 100 parts by weight of the slaughtered blood decomposed through the decomposing step and at a temperature of 30 to 35 DEG C for 2 to 3 days,
The microorganism is composed of Lactobacillus plantarum CU03 KACC 91103,
Wherein the additive is composed of 100 parts by weight of molasses, 0.25 to 0.35 parts by weight of salt, 0.05 to 0.1 parts by weight of brown sugar, and 0.05 to 0.1 parts by weight of kiwi juice.
The method according to claim 1,
The fertilizer component mixing step may comprise mixing 15 to 25 parts by weight of water, 5 to 7 parts by weight of nitrogen, 1 to 3 parts by weight of phosphorus, 1 to 3 parts by weight of potassium, and 1 to 3 parts by weight of a mineral compound And 0.01 to 0.1 part by weight of a slaughter blood.
The method according to claim 1 or 7,
Wherein the mineral mixture is composed of boric acid and molybdenum.

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