KR20180003699A - A new plant type rice having high anthocyanin contents and cultivation method thereof - Google Patents

Abstract

The present invention relates to a new variety of rice with high content of anthocyanin, a method for breeding the new rice variety with high content of anthocyanin including a step for artificially breeding Hanarum rice (a specific variety of rice) and early picked glutinous black rice, and a method for cultivating rice with increased content of anthocyanin including a step for cultivating the new rice variety. The new rice variety contains anthocyanin, which is highly antioxidant functional substance, 2.5 times higher than early picked glutinous black rice, thereby highly applicable as healthy foods such as mixed steamed rice. In addition, the new rice variety can also be useful for processing various functional foods such as production of black rice chocolate.

Description

[0001] The present invention relates to an anthocyanin-containing high yield anthocyanin content and cultivation method thereof,

The present invention relates to an anthocyanin-rich new crop variety and an improved method of cultivating the same. More specifically, the present invention relates to an anthocyanin-rich new crop variety rice containing anthocyanin- A method of breeding, and a method of cultivating rice with an increased content of anthocyanin including the step of growing the new variety rice.

One of the world's three largest food crops, rice is a crop that is used by stocks in Asia, including Korea. Accordingly, the development of rice varieties until now has been a major goal of improvement in yield. However, due to the changes in the social and economic conditions due to the improvement of the income level, interest in health has risen more than quantity and taste, and the demand for functional varieties has increased.

As an alternative to the development of varieties for the niche market of functional specialty rice, recently, the institute of the local autonomous organization has been optimally adapted to the cultivation environment of each region and the development of specialized local brand special rice varieties has progressed. And the shinto black rice of Jeollabuk-do were developed. However, the above-mentioned varieties have a problem in that they have significant differences in the content of effective functional ingredients depending on the cultivation area or environment, and thus it has been necessary to supplement the cultivation method for high quality functional specialty rice.

In particular, the color-developed rice cultivars have a disadvantage in that the content of the functional ingredient varies greatly depending on the cultivation environment such as the ecology of early-stage and middle-aged, the time of transition and the cultivation area (Oh Sung Hwan et al., 2015, J. Agr. Sci. (2), 143-149). In order to improve this, there have been developed high antioxidant black rice Milyang 290 (Korean Patent Laid-Open No. 2016-0027850) in which black rice gene and red rice gene are simultaneously expressed. However, due to lack of low yield and stability of cultivation, It is still not enough to meet.

On the other hand, oxidation is a phenomenon in which various unsaturated fatty acids in the body are oxidized by oxygen, resulting in an increase in lipid peroxidation, and an aging phenomenon such as DNA damage is promoted. Oxidation of various types of radicals (O ₂ ^- , superoxide, nitric oxide, NO ₂ , hydroxyl, ROO, proxyl, RO, alkoxyl and NO ₃ peroxynitrite) . Antioxidant activity is a phenomenon that protects cells from oxidative damage by removing free radicals from harmful radicals such as antioxidant enzymes, superoxide dismutase (SOD), catalase (GSHpx), and glutathione.

As described above, black rice, which is one kind of color pigments, has various antioxidant activities as anthocyanin and reddish polyphenol are deposited on the seed coat. In particular, black rice has been known to show effects such as heart disease, stroke, adult disease, cancer prevention through high antioxidative effect, and consumers are receiving high attention. Therefore, it is necessary to develop rice cultivars which express high amounts of anthocyanin and cultivation methods that minimize changes in their expression depending on cultivation environment.

Under these circumstances, the present inventors have made extensive efforts to develop new cultivars capable of developing anthocyanin-rich new crop varieties, further improving their contents, and minimizing changes in anthocyanin content depending on the cultivation environment. As a result, It was confirmed that anthocyanin content of the new cultivated rice selected by artificial crossing of the early insecticide was high and that the content of anthocyanin was greatly increased when the cultivated cultivar was cultivated in the new cultivar or general cultivar.

One object of the present invention is to provide an anthocyanin-rich new crop variety rice.

Another object of the present invention is to provide a method of breeding the new variety of rice.

Another object of the present invention is to provide a method for cultivating rice with an increased content of anthocyanin including the step of growing the new variety rice.

As one embodiment for achieving the above object, the present invention provides an anthocyanin-rich new variety rice plant deposited with the deposit number KACC 98047P.

The deposited new crop variety of the present invention may have the following botanical characteristics: heading on August 20 to September 5; Liver of 70 to 85 cm; A head of 15 to 20 cm; 15 to 20 transmutations; 75 to 95 allowances to be established; A brown rice yield of 450 to 550 kg / 10a; A protein content of 4 to 6%; Amylose content of 15 to 25%.

The term "anthocyanin" of the present invention refers to a pigment mainly contained in flowers and fruits, and is characterized by a flavonoid-based substance with almost no odor and taste. Anthocyanin inhibits the oxidation of cells by eliminating reactive oxygen species that are generated in cells, and thus has an advantageous effect on health such as anti-aging or immunity enhancement.

Specific examples of the anthocyanin include delphinidin, cyanidin, petunidin, pelargonidin, peonidin, or malvidin, Anthocyanin may be cyanidin, specifically cyanidin-3-glucoside (C3G), but is not limited thereto.

The seeds of the new variety of rice of the present invention may contain anthocyanin in the range of 200 to 350, particularly 250 to 300 mg / 100g when the nutrient-free treated seeds are cultivated; It can be included in the content of 800 to 950, specifically 850 to 900 mg / 100g in nutrient treatment cultivation, but is not limited thereto.

In the present invention, the nutrient may be "florone ".

The floren is a nutritional product manufactured and sold by Atlantica Agricole, and is a growth-converting functional product that converts the nutritional growth of a plant into a reproductive growth, and is known to have excellent fertilization, suppression of coating and fruitful enlargement.

According to one embodiment of the present invention, the content of cyanidin-3-glucoside contained in the brown rice sample of the new variety of the present invention was 262.2 mg / 100g for the nutrient-treated rice, The cultivated rice contained 886.0 ㎎ / 100g of anthocyanin (Table 3).

The new variety of rice of the present invention KACC 98047P on Apr. 18, 2016 at the National Institute of Agricultural Science and Technology in Suidong-dong, Gyeonggi-do, Suwon-gu, Gyeonggi-do. The new variety of rice is readily available through the above-mentioned engines.

In another embodiment, the present invention provides a method of breeding a new variety of anthocyanin-rich rice plants comprising the step of artificially crossing the cultivars of japanese rice and early-stage black rice, cultivating artificially crossed rice, and harvesting the later rice .

At this time, the harvested late rice is not particularly limited as long as it contains high anthocyanin in its seed, but may be, for example, a new variety of rice deposited with deposit No. KACC 98047P.

In a specific embodiment of the present invention, artificial crosses were carried out using the early breed, which is a short-season rice and early-born rice, as a part of the short-breed variety Milyang 227, whose free sugar content is about 7 times higher than that of ordinary rice. F2 and F3 of the above-mentioned sample and the replicate were respectively cultured, and the system YR26501-17-1-2 fixed by the system breeding method was selected from the F4 generation to the F6 generation in the Jindo group test package. As a result of the preliminary selection test for productivity test and the 2-year productivity test, it was confirmed that the anthocyanin content was high and the seedling cultivation was high. Therefore, the date of April 18, 2016 to the National Institute of Agricultural Science, As a deposit number KACC 98047P.

In another embodiment, the present invention provides a method for cultivating rice with an increased content of anthocyanin, comprising culturing the new variety of rice.

The cultivation method may further include a step of treating the rice with the nutrient before harvesting after cultivating the new variety of rice. At this time, fluoren may be used as the nutrient.

The concentration, treatment amount and treatment method of the fluorene can be appropriately adjusted to achieve the object of the present invention.

Specifically, the concentration of the fluorene may be 0.0001 to 0.001% (v / v), more specifically 0.000625% (v / v), but is not limited thereto.

In addition, the florene may be sprayed for a total of one to three times, specifically two times before harvesting the rice, more specifically once 40 to 50 days before; And 25 to 35 days prior to application.

The florene may be applied by spraying 100 to 300 L, specifically 150 to 250 L per 10 a cultivation area of rice before harvesting the rice, but the present invention is not limited thereto.

The new variety of rice of the present invention has a high antioxidant activity and anthocyanin content of about 2.5 times higher than that of early-stage insecticide. Therefore, it is very useful as health food such as mixed rice, and can be usefully used for various functional food processing have.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart of a new variety of rice (YR26051-17-1-2) of the present invention.
Fig. 2 is a line drawing of a new variety of rice of the present invention.
Fig. 3 is an image showing the growth phase of a new variety of rice of the present invention. Fig.
4 is an image showing the brown rice appearance of the new variety of rice of the present invention.
Figure 5 is a chromatogram showing the anthocyanin content of the new variety of rice of the present invention.

Hereinafter, the present invention will be described more specifically in the following examples. However, these examples are provided only to aid understanding of the present invention, and the present invention is not limited thereto.

Example  1. How to breed new varieties of rice

In order to develop a functional new crop variety with high anthocyanin content, we cultivated a mature soybean cultivar, Milyang 227, which is about 7 times higher in free sugar content than ordinary rice, and artificially cultivated black rice, .

Specifically, Milyang 277, which has a low sweetness of starch, was used as the mating sample. The artificial mating was carried out in the summer of 2006 by using early - born insect, In the summer of 2008 and 2009, F2 and F3 were cultivated, respectively, and the YR26501-17-1-2 strain selected from the F4 generation in FY2010 to the F6 generation in FY2010 . As a result of the preliminary selection test for productivity test in 2013 and the two-year productivity test from 2014 to 2015, it was confirmed that the planting stability and the anthocyanin content were high.

The new varieties of the present invention were deposited with the National Agricultural Research Service Agricultural Genetics Center, Seodun-dong, Gwon Seon-gu, Gyeonggi-do, Korea, under the deposit number KACC 98047P dated April 18,

The cultivation scheme of the new variety of rice and the cultivation diagram of the new variety are shown in Fig. 1 and Fig.

Example  2. Analysis of Growth Characteristics of Newly Produced Rice

Growth characteristics including the agricultural characteristics, tolerance to diseases and tillage characteristics of the newly cultivated rice were investigated at the National Crop Science Division of the National Institute of Food Science for two years from 2014 to 2015.

Specifically, the seeds of each line were sown in a seedling box according to the RDA, and after 30 days of seedlings, they were transplanted on June 5. The planting distance was 30 ㎝ (hot distance) × 15 ㎝ (daytime distance), and 3 trees were planted per week. Nitrogen fertilizer, fertilizer ratio and fertilizer ratio were measured at 50%, 20% and 30%, respectively. Nitrogen fertilizer, calcium fertilizer and potassium fertilizer were distributed at the ratio of N-P2O5-K2O = 9-4.5-5.7㎏ / The defense was divided into 70% and 30%. The pest management and other cultivation management were based on the standard cultivation law of RDA and the characteristics such as major agricultural traits such as heading date, yield component and yield were investigated in accordance with the research standard of RDA.

The results of analysis of the agricultural characteristics, tolerance to disease, physicochemical properties and content of functional materials of the new variety were confirmed in the following Examples 2-1 to 2-4.

Example  2-1. Identification of agricultural characteristics of new variety rice

Breed name Heading machine ^{z )}
(Mon. Sun.) Soy sauce
(cm) stripe
(cm) Sorghum
(dog) Allowable lips
several) Maturity rate
(%) Suit
(1-9) Brown rice quantity
(kg / 10a) Indices Miryang magnitude New variety rice 8.22 8.24 78 18 17 81 84.3 One 486 98 Early Morning 8.10 8.14 71 23 13 97 32.5 One 493 100

^z) Date of transition (Mon. and Sun.): Miryang (6.5), Jindo (6.17)

As shown in the above Table 1, in the case of the newly grown variety rice, it was confirmed that the emergence period was the mid-life on August 22, June 23rd, June 17th from August 23rd to June 24th. Soy sauce was 78 ㎝ in diameter, about 7 ㎝ larger than the early breed, which is the standard variety. The length of the soy sauce was shorter than that of the early-stage, and the number of syringes was 17, Also, the ripening rate was 84.3%, there was no clothing in the packaging, and the yield of brown rice was 486kg / 10a, which was about 2% of that of the early breed.

FIG. 3 shows the ripening phase of the new variety rice having the above agricultural characteristics.

Example  2-2. Confirmation of loneliness of new crop variety rice

Breed name leaf
Blast
Blight of blight Viral disease Brownfly Leo K ₁ K ₂ K ₃ K _3a stripe
Leaf blight Orgasm Black line
Orgasm New variety rice about about about about about about about about about about Early Morning about about about about about about medium about about about

As shown in Table 2, it was confirmed that the new variety of rice was susceptible to various pests, as in the case of the early breed, which is a standard variety. Specifically, the new variety Rice was susceptible to leaf blast and susceptible to all strains of K ₁ -K _3a . Were susceptible to viral pathogens such as stripe blight, mildew disease, and black streak mite, and were also susceptible to brown planthoppers and lycopodia.

Example  2-3. Physicochemical Properties and Anthocyanin of New Rice Plants C3G ) Content analysis

In order to analyze the physicochemical properties such as amylose content and protein content of the new variety of the present invention, samples cultivated at Milyang National Food Research Institute in Gyeongsangnam-do were used. Anthocyanin as a main functional substance, specifically cyanidin-3-glucoside -3-glucoside; C3G) was used for the analysis, and UPLC (waters, ACQUITY) instrument was used for analysis.

Specifically, in order to cultivate a new variety of rice functional material in a high content, a nutrient (Floren, Atlantica agricola) was diluted to 1600 times (250 ml of a nutrient / 400 liters of water) (30 days and 45 days before).

After that, new variety rice or early - stage black rice was harvested 35 ~ 45 days after heading. After harvesting the rice, it was naturally dried to a moisture content of 15% or less and treated with a laboratory microscope (SsangYong SY88-TH). The brown rice or white rice removed from the rice was removed, Were used for the analysis.

Amylose content was analyzed by the following method. 100 mg of brown rice flour was mixed with 1 ml of 95% ethanol and 9 ml of 1N NaOH for 10 minutes in boiling water, and then 100 ml of distilled water was added. 5 ml of the mixture was extracted with 1 ml of 1N acetic acid and 2% 2 ml of a potassium iodide solution (I2-KI solution) was added and the mixture was filled up to 100 ml with distilled water. Then, the absorbance was measured by a spectrophotometer at a wavelength of 620 nm to calculate the amylose content.

Protein content was determined by Kjeldahl nitrogen determination method. Specifically, the amount of protein was determined by multiplying the nitrogen conversion factor by 5.95 by the nitrogen conversion factor.

The content of anthocyanin (C3G) was extracted with 1 g of brown rice samples and 20 ml of extraction solvent (1% HCl in 80% MeOH) using a constant temperature water bath (30 ° C, 24 hours). The analytical instrument used was UPLC (waters, ACQUITY) and the column was a Waters ACQUITY BEH C18 column (particle size 1.7 mm, 2.1 × 50 mm, 35 ° C.). The PDA detector (530 nm) was used as the detector. TFA (in D.W (A)) and TFA (in MeOH (B) were used as the mobile phase and the sample temperature was 15 ° C.

The measured amylose, protein, and anthocyanin (C3G) contents and the like are summarized in Tables 3 and 4 below.

Physicochemical properties and anthocyanin (C3G) content of new varieties Breed name Protein content
(%) Amylose Content
(%) Anthocyanin (C3G)
(mg / 100g) Nutrient treatment Nutrient-free treatment New variety rice 5.1 21.1 886.0 262.2 Early Morning 5.9 7.3 358.7 105.1

Detailed contents of anthocyanin (C3G) year kind Nutrients C3G content (mg / 100g) One 2 3 Average 2014 New variety rice process 727.9 746.4 801.2 758.5 No treatment 222.9 222.2 230.8 225.3 Early Morning process 319.0 312.7 291.2 307.6 No treatment 101.4 89.2 86.3 92.3 2015 New variety rice process 746.4 801.2 1492.8 1013.5 No treatment 222.2 230.8 444.4 299.1 Early Morning process 312.7 291.2 625.5 409.8 No treatment 89.2 86.3 178.4 118.0 system New variety rice process 737.2 773.8 1147.0 886.0 No treatment 222.5 226.5 337.6 262.2 Early Morning process 315.9 302.0 458.3 358.7 No treatment 95.3 87.8 132.4 105.1

As shown in Tables 3 and 4, the content of anthocyanin (C3G), which is a major functional ingredient of the new variety, is 262.2 mg / 100 g, which is about 2.5 times higher than that of the early- Respectively. In particular, when the foliar application was carried out twice at 30 days and 45 days before harvest, the anthocyanin (C3G) content was 886.0 mg / 100g, which is about 3.4 times higher than that of untreated and 8.4 times higher than that of the early cultivation.

On the other hand, the protein content of the new variety of the present invention was lower than that of the control soybean pheromone, 5.1%, and the amylose content was about 21.1%. FIG. 4 shows the brown rice appearance of the newly cultivated rice having such physicochemical properties and high anthocyanin content. By confirming that the degree of deposition of anthocyanin (C3G) pigment is much higher in the brown rice of the new variety rice than that of the early cultivar, It was found that the function of the new variety rice was excellent.

As can be seen from FIG. 5, the analysis of anthocyanin (C3G) content of the new variety of rice showed that the degree of deposition of the brown rice pigment of the new variety was much higher than that of the early type of the old variety, And the sex was very excellent.

Korea Agricultural Microbiology Resource Center KACC98047P 20160418

Claims (12)

Accession number KACC 98047P deposited with anthocyanin-rich new crops.
The method according to claim 1,
Wherein the anthocyanin is cyanidin-3-glucoside (C3G).
The method according to claim 1,
Wherein the anthocyanin is contained in an amount of 200 to 350 mg / 100 g when the nutrient is not treated.
The method according to claim 1,
Wherein the anthocyanin is contained in an amount of 800 to 950 mg / 100 g in the nutrient-treated cultivation.
The method according to claim 1,
The above-mentioned new variety rice paddies were cultivated in a variety of cultivars such as cultivars, And early-stage inscription were artificially cross-breeded.
A method of breeding an anthocyanin-rich new crop variety rice, comprising culturing artificially crossed rice and artificially crossing the cultivars of Korean horse radish and early-stage black rice, and harvesting the later rice.
The method according to claim 6,
Wherein the latter rice is a new variety of anthocyanin-rich rice deposited with the deposit number KACC 98047P.
A method for cultivating rice with an increased anthocyanin content, comprising cultivating the new variety rice of any one of claims 1 to 5.
9. The method of claim 8,
Further comprising the step of treating the rice with the nutrient before harvesting after cultivating the new variety of rice.
10. The method of claim 9,
Wherein the treatment concentration of the nutrient is 0.0001 to 0.001% (v / v).
10. The method of claim 9,
The nutrient may be administered once 40-50 days before harvest; And once 25-35 days prior to treatment.
10. The method of claim 9,
Wherein the nutrient is treated with a spraying amount of 100 to 300 L per 10 < RTI ID = 0.0 > a < / RTI >

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