KR20170119460A - Artificial rumen model equipment - Google Patents

Abstract

The present invention relates to an artificial rumen model device, and more particularly, to an artificial rumen model device, which comprises a main fermenter, a connection pipe discharged according to a water surface height inside the fermenter, a cylinder connected to the correlation, and a cylinder brush, To an artificial rumen continuous culture system comprising an inlet. Particularly, the feed to be fed is formed into a pellet shape including a forage, and includes an automatic feeder in which the feed is stored vertically on the feed port and is automatically supplied with time. According to the present invention, it is possible to evaluate rumen fermentation characteristics, rumen digestibility and nutritional value of feed and feed ingredients, feed additive, and automation of feeding device, automatic recording of temperature and pH, It provides a system developed to improve the welfare of researchers and increase the reproducibility of research results.

Description

[0001] Artificial rumen model equipment [0002]

The present invention relates to an artificial rumen model device, and more particularly, to an artificial rumen model device, which comprises a main fermenter, a connection pipe discharged according to a water surface height inside the fermenter, a cylinder connected to the correlation, and a cylinder brush, To an artificial rumen continuous culture system comprising an inlet.

Since ruminants use VFA (volatile fatty acid), which is decomposed by microorganisms in the rumen, as an energy source, unlike unit animals, fermentation in rumen greatly affects the productivity of fowl. Therefore, in order to investigate the fermentation characteristics and feed utilization of rumen rumen, in vitro studies and in vivo studies are underway. In vitro experiments using ruminal juice were performed by in vitro batch cultures (Miler and Wolin, 1974), in which an artificial saliva (McDougall's buffer) in a serum bottle and anaerobic culture Is widely used. However, this method differs from the in vivo fermentation phase which takes place in the actual rumen, which makes it difficult to actually evaluate the digestibility and the value of the nutrients in the rumen. In addition, in vivo methods directly measure the nutrient digestion of feed in animals, so it takes a lot of time and money (Van Straalen and Tamminga, 1990). Therefore, In order to overcome the disadvantages of the batch culture and the in vivo experiments, various studies on the rumen continuous culture system are under way.

Muetzel et al. Used a model developed by Muetzel et al., But there is no commercially available model and lab scale model makes it difficult to operate and manage research equipments. In particular, conventional devices are not automated, so labor force is required to feed experimental feed, conduct research, and collect samples.

Korean Patent No. 1449144 discloses a continuous rumen model continuous culture system, but there is no description of an artificial rumen model including an automatic feed feeder of the present invention and an outlet clog prevention device.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems,

By automating the feeder and buffer feeder, it compensates for the disadvantages of the conventional manual artificial rumen model. The automatic recording of temperature and pH and the system to prevent the clogging of the outlet block can reduce the labor force and improve the welfare of researchers. The objective of this study was to develop an artificial rumen modeling system to verify the fermentation characteristics of rumen, rumen digestibility and nutritional value of raw feed and feed additives.

To achieve the above object, the present invention provides a fermentation apparatus comprising: a fermentation tank having a discharge port formed at one side thereof and a feed port formed at the other side thereof;

A fully automatic cylinder brush device connected to a discharge port of the fermenter to prevent the inside of the discharge port from being clogged;

And an automatic feed input device connected to a feed inlet of the fermenter to feed the feed into the fermenter. The present invention also relates to an artificial rumen model device.

Further, the fermenter of the present invention is characterized in that the interior thereof is hollow so as to open the upper part, and a lid which opens and closes the hollow interior is formed in the opened upper part.

A buffer inlet, a gas inlet, a pH meter inlet, a DO sensor inlet, a thermometer inlet, and a gas outlet are formed on one end surface of the lid, respectively, and a stirrer directly connected to a stirring rod formed up to the inside of the fermenter is installed in the lid of the present invention. To the artificial rumen model device.

In addition, the discharge port of the present invention is formed to be inclined in the upper diagonal direction, and an automatic cylinder brush device is connected to an end portion of the discharge port, and a discharge pipe that branches in a lower diagonal direction is protruded from one end of the discharge port. .

In addition, the present invention relates to an artificial rumen model device, which is connected to a sample storage device in which a rumen overflowed in a fermentation tank is transferred and stored.

In addition, the sample storage device of the present invention is surrounded by a jacket on the outer periphery, and cooling water is circulated inside the jacket and connected to a constant temperature water tank for circulating the cooling water.

Further, the buffer injection port of the present invention is connected to a bottle containing a buffer by a flow pipe, and relates to an artificial rumen model device.

In the gas outlet of the present invention, gas generated by fermentation is discharged from the fermenter, and the gas generated by the flow tube connected to the tether bag is collected in the tether bag. .

Further, the fully automatic cylinder brush device of the present invention comprises: a brush cylinder which is detachably attached to an outlet of the fermentation tank;

A cleaning brush connected to one side of the brush cylinder and provided in an outlet of the fermentation tank for performing a piston motion by the brush cylinder to clean the inside of the outlet of the fermentation tank;

And a motor which is formed on the other side of the brush cylinder and operates the brush cylinder according to setting conditions.

Further, the automatic feed feeder of the present invention comprises: a feed storage unit connected to the feed inlet of the fermenter vertically and storing pellet-shaped feed therein;

A feed inlet cylinder connected to a feed inlet of the fermenter;

A feed hole is formed in the feed inlet of the fermentation tank so as to support the feed dropped from the feed storage unit at a lower end of the feed storage unit and connected to one side of the feed feed cylinder, Wherein the feed hole is transported to the inside of the fermenter and the feed located in the feed hole is removed from the inside of the fermenter;

And a motor formed on the other side of the feeding cylinder to operate the feed feeding cylinder in accordance with setting conditions.

As described above, the artificial rumen model device of the present invention can confirm the fermentation property by obtaining the fermentation product generated from the rumen, further automating the feeding device, automatically recording the temperature and pH, and automating sampling It has the effect of reducing the labor force, improving the welfare of researchers, and increasing the reproducibility of research results.

Also, there is an effect of providing a system that can easily study the functional aspects of rumen in vitro by improving feed inlet clogging and outlet clogging, which are disadvantages of conventional models, through an automatic feed feeder and a cylinder brush.

1 is a front view showing a main fermenter according to an embodiment of the present invention,
2 is a plan view of a lid of a fermenter according to an embodiment of the present invention,
3 is a front view showing a fermenter with an automatic feed input device according to an embodiment of the present invention,
4 is a schematic view showing the operation of the automatic feeder according to the embodiment of the present invention,
5 is a front view of a fully automatic cylinder brush device according to an embodiment of the present invention,
6 is a front view showing a sample storage device according to an embodiment of the present invention,
7 is a front view showing an artificial rumen modeling apparatus according to an embodiment of the present invention.

The present invention having such characteristics can be more clearly described by the preferred embodiments thereof.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Before describing in detail several embodiments of the present invention with reference to the accompanying drawings, it is to be understood that the present invention is not limited to the details of construction and the arrangement of components shown in the following detailed description or illustrated in the drawings will be. The invention may be embodied and carried out in other embodiments and carried out in various ways. It should also be noted that the device or element orientation (e.g., "front," "back," "up," "down," "top," "bottom, Expressions and predicates used herein for terms such as "left," " right, "" lateral, " and the like are used merely to simplify the description of the present invention, Or that the element has to have a particular orientation. Also, terms such as " first "and" second "are used herein for the purpose of the description and the appended claims, and are not intended to indicate or imply their relative importance or purpose.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and do not represent all the technical ideas of the present invention. Therefore, It is to be understood that equivalents and modifications are possible.

FIG. 1 is a front view showing a main fermenter according to an embodiment of the present invention, FIG. 2 is a plan view showing a lid of a fermenter according to an embodiment of the present invention, FIG. 3 is a cross- FIG. 4 is a schematic view showing the operation of the automatic feeding device according to an embodiment of the present invention, and FIG. 5 is a view showing a fully automatic cylinder brush device according to an embodiment of the present invention. FIG. 6 is a front view showing a sample storage device according to an embodiment of the present invention, and FIG. 7 is a front view showing an artificial rumen modeling apparatus according to an embodiment of the present invention.

1 to 7, the artificial rumen model device of the present invention includes a fermentation tank 100 having a discharge port 200 formed at one side thereof and a feed port 130 formed at the other side thereof; A fully automatic cylinder brush device 230 connected to the discharge port 200 of the fermenter 100 to prevent the inside of the discharge port 200 from being clogged; An automatic feed input device 500 connected to the feed inlet 130 of the fermentation tank 100 to feed the feed into the fermentation tank 100 and an artificial rumen sample storage device 600 and a control unit 700 .

As shown in FIGS. 1 to 3 and 7, the fermentation tank 100 is made of stainless steel in order to facilitate the management of the fermentation tank 100 and to prevent breakage. The fermentation tank 100 includes an observation window 110 Install it. The fermenter 100 maintains the temperature of the fermenter 100 in a hydrothermal circulation manner through a constant temperature water tank installed on the outside of the stainless steel jacket 120 and separates all the components for washing and sterilization of the fermenter 100 itself Is designed. At this time, the fermenter (100) has a capacity of 1.5 L and a total capacity of 3 L.

The outlet (200) connected to the fermenter (100) is fabricated so that the fermentation product can overflow by feeding the buffer at a culture capacity of 1.5L. In addition, the discharge port 200 is provided with a clogging prevention device by applying the brush cylinder 250 used in the automatic feed input device 500 to solve the clogging of the discharge port 200, which may occur due to high rumen characteristics do.

The outlet 200 is inclined upward in the diagonal direction and the automatic cylinder brush device 230 is connected to an end of the discharge port 200. A discharge pipe 220 branched in the lower diagonal direction is protruded from one end of the discharge port 200 . At this time, the lower pipe 220 of the discharge port 200 is connected to the sample storage device 600 in which the overflowed rumen from the fermentation tank 100 is transferred and stored.

The lid 300 of the fermentation tank 100 is connected to the stirrer 400 and the lid 300. The lid 300 is connected to the stirrer 400, The stirrer 400 of the artificial rumen is provided with a screw type stirrer 400 for smooth stirring at a low speed in the fermenter 100. The stirrer 400 is attached to the fermenter lid 300, And a pH measuring rod inlet 350 and a dissolved oxygen amount measuring rod inlet 340 are formed in the upper portion of the reactor 300. A buffer inlet 360, a carbon dioxide and nitrogen gas inlet 380, a thermometer inlet 370 and a gas outlet 320 are formed . In this case, a motor for rotating the stirrer 400 attached to the fermentation tank lid 300 is provided at the upper end of the fermenter in a prefabricated form, and the stirrer 400 rotates the rotation speed of the stirrer 310 from 0 to 120 rpm .

The buffer input port 360 is connected to a bottle 760 containing a buffer. The bottle 760 is connected to the fermentation tank 100 through a fluid pipe. The charging rate is controlled through a peristaltic pump 750, The peristaltic pump 750 supplies the buffer in an amount of 0.75 to 2.5 ml / min.

In order to prevent water evaporation in the fermentation tank 100, the gas outlet 320 is maintained at a predetermined temperature by a cold water circulating type constant temperature water tank provided with a condenser 420. The material of the fermenter lid 300 is also made of stainless steel, and all the devices can be separated. A pH measuring rod, a dissolved oxygen measuring bar, a buffer inlet pipe, a gas inlet pipe, a gas outlet, and a thermometer are all designed to be detachable from the lid 300 of the fermenter.

The fermentation tank 100 is connected to a constant temperature water tank that is kept warm by the metal jacket 120 and circulates hot water therein and the temperature of the constant temperature water tank connected to the fermentation tank 100 is maintained at 37 to 39 ° C do.

The gas introduced through the gas inlet 380 is connected to the inside of the fermenter 100 by a fluid pipe. The gas is connected to a gas input control valve (not shown), and the gas is supplied to the inside of the fermenter 100 through carbon dioxide or nitrogen (Not shown).

Meanwhile, the gas generated by the fermentation in the fermentation tank 100 is discharged through the gas outlet 320, and is collected by the fluidizing tube connected to the thermistor bag.

3 to 4, the automatic feed feeder 500 is connected to the feed inlet 130 of the fermenter 100 and includes a pellet feed storage (510); A feed inlet cylinder 520 connected to the feed inlet 130 of the fermenter 100; The feed storage unit 510 is connected to one side of the feeding cylinder 520 and is disposed inside the feed inlet 130 of the fermentation tank 100. The feed stored in the feed storage unit 510 is positioned at the lower end of the feed storage unit 510, A feed hole 560 is formed in the feed hole 560 so that the feed hole 560 is moved by the feeding cylinder 520 and the feed hole 560 is fed to the inside of the fermentation tank 100. At the same time, (550) which is detached from the inside of the feeder (100); And a motor 530 formed on the other side of the feeding cylinder 520 for operating the feed feeding cylinder 520 in accordance with setting conditions.

Here, the automatic feeder 500 is designed to feed a pellet-shaped feed in a vertically-collapsed manner and feed a certain amount of feed in a predetermined time. In addition, the feeding device 500 is designed to input the desired time and amount to be fed to the main controller since the feeding cylinder 520 is connected to the controller 700. Feeds fed into the fermenter include press-type stainless steel molds for processing forage and concentrated feed. The roughage and the concentrated feed are crushed, blended at a predetermined ratio, added with moisture, dried in a dry oven, and put into an automatic feed storage unit 510.

Reference numeral 530 denotes a motor, and reference numeral 540 denotes an electric connection line.

1 and 5, the fully automatic cylinder brush device 230 includes a brush cylinder 250 detachably attached to a discharge port 200 of the fermenter 100; Is connected to one side of the brush cylinder 250 and is provided inside the discharge port 200 of the fermentation tank 100 and performs piston motion by the brush cylinder 250 to clean the inside of the discharge port 200 of the fermentation tank 100 Cleaning brush 240; And a motor 260 formed on the other side of the brush cylinder 250 to operate the brush cylinder 250 in accordance with setting conditions.

A brush 230 is installed at a front portion of the cylinder 250 and is designed to automatically clean the fermentation tank 100 and the discharge port 200 through a piston movement according to a preset time have. That is, the brush 240 moves from the discharge port 200 to the inside of the fermentation tank 100 to clean the inside thereof.

6 to 7, the sample storage device 600 may be opened or closed through a lid 610 to block the inflow of all the air. The lid 610 of the sample storage device 600 may be made of stainless steel. The sample storage device 600 is made of glass, and is surrounded by a jacket 620 and circulates cooling water therein, and is connected to a constant temperature water tank for circulating cooling water.

In this case, the constant temperature water tank connected to the sample storage device 600 is maintained at a temperature of 3-4 ° C., and the sample storage device 600 includes a lid 610, and can block the inflow of all the air.

Reference numeral 631 denotes a cooling water circulation connection 1, 632 denotes a cooling water circulation connection 2, 640 denotes an opening and closing screw, 650 denotes a sampling port, 660 denotes a sample storage device connection site, and 670 denotes a sampling connection pipe.

7, the control unit 700 is connected to the fermentation tank 100, and controls the amount of the buffer, amount of feed, the cylinder brush 240 connected to the discharge port 200, The rpm of the agitator, and the overall on / off of the device.

The control unit 700 can periodically check the pH, the DO, the internal temperature of the fermenter, the internal temperature of the sample storage, and the rpm of the agitator through the monitor 710 attached to the device. And can automatically monitor pH, DO, fermenter internal temperature, sample storage internal temperature and stirrer rpm and store the data in USB. At this time, the controller 700 may set the entire automatic or manual operation of the device.

As shown in FIG. 7, in the artificial rumen model device of the present invention, the buffer continuously fed into the fermentation tank 100 is supplied from the outer side of the fermentation tank 100 through a connection pipe, and the connection pipe is a pump 750 installed in the artificial rumen device ) Into the fermenter (100). A total of three buffer pumping pumps 750 are installed in the artificial rumen device, and all of the pumps are connected to the body control unit 700 to set the amount of the pump, and are automatically supplied. The charged buffer overflows the fermented fermented product in the fermentation tank 100 and is stored in the sample storage device 600 through the outlet 200 to control the pH of the fermentation tank 100. The storage of the buffer may be a glass sample bottle 760, and the connection pipe connecting the bottle 760 and the fermentation tank 100 may be a silicon tube. The incubation conditions can be input to the control unit 700 located at the upper left of the device and can be automatically operated. All the information on the incubation is automatically stored. The artificial rumen operation and real-time monitoring are performed through the touch- It is possible. In addition, the stored culture information can be connected to the USB through a USB port installed in the main body, and the stored culture information can be stored and checked by an external control unit 700. All the operations are operated by the control unit 700 to enable more accurate and convenient operation.

Unsigned 720 is pH / D.O. A sensor panel 730, a power supply 740, an operation indicator 770, and an internal storage 770.

Hereinafter, an experimental method and an experimental result using an artificial rumen modeling apparatus according to an embodiment of the present invention will be described.

First, the preparation method of the buffer and feed pellet to be used in the experiment will be described.

The buffer used in the experiment is called McDougall's buffer (McDougall, 1948) and is referred to as artificial saliva. The addition components and amounts of 1L are shown in [Table 1] below.

ingredient Addition amount (g) NaHCO ₃ 9.8 Na ₂ HPO ₄ 7H ₂ O 7.0 KCl 0.57 NaCl 0.47 MgSO ₄ 7H ₂ O 0.12 CaCl ₂ 0.04

After adding all of the above ingredients, thoroughly dissolve and thoroughly mix. After adjusting the pH of the buffer to 7.0, it is anaerobically injected with carbon dioxide and nitrogen gas.

Feed pellets are ground and pulverized to 1 mm and then dried and mixed at a constant ratio. After that, water is added and put into a feed fixture and pressed to form a pellet. The molded pellets are dried in a dry oven at 60 ° C until the water is completely removed.

The initial culture was prepared by firstly filtering the gastric juice taken directly from the rumen equipped with a cannula into a 4-ply cheese cloth, filtering it again with a 4-ply cheese cloth before being injected into the fermenter (100) Carbon dioxide and nitrogen gas were sufficiently injected to maintain the anaerobic state. After the sterilized fermentation tank 100 is installed, the automatic feed feeder 500 is connected to the fermentation tank 100, and the outlet 200 and the sample storage device connection pipe sample storage device 600 are installed . In the sample storage device 600, the inflow of air is completely closed by the lid 610, and the temperature is set to 4 ° C in advance by using the constant temperature water bath. Thereafter, the rumen juice diluted with artificial saliva and cheese cloth prepared in advance is mixed at a ratio of 1: 1 (750 ml: 750 ml), and is put into the fermenter (100) while maintaining the anaerobic state. The lid 300 of the fermenter is closed and a pH measuring rod, a dissolved oxygen meter, a thermometer, a buffer inlet pipe and a gas inlet pipe are connected to each other and a Tedlar bag is connected to the gas outlet port 420, Prepare. Then, the control unit 700 inputs the culture conditions. First, carbon dioxide and nitrogen were injected to maintain the anaerobic condition for 30 minutes to 1 hour. Then, all the apparatuses were closed to prevent air inflow. Then, the gas injection was stopped. To stabilize the microorganisms, Cultivation is carried out at a stirring speed of 39 to 40 DEG C and 120 rpm or less without adding. After 12 hours from the initiation of the culture, 15 to 30 g of the pellet-shaped feed are put into the fermentation tank and incubated at 39 ° C at a stirring rate of 10 to 120 rpm for anaerobic fermentation under no anaerobic fermentation conditions. Feeds were fed 7-15g once every 12 hours twice a day, and the buffer was added at about 0.75-1ml per minute (1080-1440ml / day). Thereafter, cultivation was performed for a total of 9 days, and samples were collected from the sample storage device 600 at intervals of 24 hours for analysis. The total amount of gas generated by the microorganisms in the fermenter 100 was collected in a Tedlar bag connected to the gas outlet 420. The amount of gas was measured using a glass syringe and the collected gas was passed through a glass column and a TCD detector And the amount of methane gas and carbon dioxide gas was measured using the GC.

The results of the above experiment were as follows.

A. pH: Initial pH of the rumen fluid at pH 6.51 was maintained at 6.22 ~ 6.54 at 9 days of total culture. The pH tended to decrease in the first 2 ~ 3 hours after feeding, and then the condition was recovered again.

B. Temperature: The temperature inside the culture was constantly maintained at 39 to 40 ° C for 9 days of the total culture period, and the stored samples in the sample storage were maintained at a temperature of 3 to 4 ° C.

C. Dissolved Oxygen: The amount of dissolved oxygen remained constant between 0 and 1% during the total incubation period, and it was confirmed that the overall culture was well anaerobicized.

D. Total amount of generated gas: It was confirmed that the amount of generated gas was 500 ml in the initial day, and 1100 to 1190 ml / day in 5 days after the culture.

E. Generation of methane gas: The amount of methane produced by GC was not significantly increased on the first day of the initial culture, but was significantly increased from 94.3 to 475.7 ml / day on day 2. Methane gas in the rumen is produced by methanogens, and most methanogens grow in anaerobic conditions. Therefore, anaerobic fermentation in the artificial rumen was relatively well maintained.

F. Carbon dioxide generation: It was confirmed that the amount of carbon dioxide gas generated by GC was 375.8 ~ 1043.9ml / day among 9 days of culture.

G. Changes in ruminal microbial protein content: The ruminal microbial protein content of the fermented samples from sample storage was measured. The initial protein content was 2.86 g / l and the incubation period was 1.44 ~ 2.11 g / l, indicating that the ruminal microorganisms were stabilized in the fermenter.

100: Fermenter 110: Observation window inside
120: Metal jacket 130: Feed inlet
200: Outlet 210: Cylinder connector
220: Lower pipe 230: Full automatic cylinder brush
240: Cleaning brush 250: Brush cylinder
260: Motor 270: Electrical connection line
300: lid 310: stir bar
320: gas outlet 330: handle hole
340: DO sensor input port 350: pH meter input port
360: buffer input port 370: thermometer input port
380: gas inlet port 390: spare port
400: stirrer 410: stirring rod connecting portion
420: Capacitor
500: Automatic feed feeder 510: Feed feeder
520 feed feed cylinder 530 motor
540: electrical connection line 550: feed delivery unit
560:
600: sample storage device 610: lid
620: Cooling water jacket 631: Cooling water circulation connector 1
632: Coolant circulation connector 2 640: Closing screw
650: Sampling port 660: Sample storage connection
670: Sampling connector
700: artificial rumen control unit 710: monitor
720: pH / DO sensor panel 730: instrument power
740: Operation indicator 750: Peristaltic pump
760: buffer bottle 770: internal storage

Claims (10)

A fermenter (100) having an outlet (200) formed on one side and a feed inlet (130) on the other side;
A fully automatic cylinder brush device 230 connected to the discharge port 200 of the fermenter 100 to prevent the inside of the discharge port 200 from being clogged;
An automatic feed feeder 500 connected to the feed inlet 130 of the fermenter 100 to feed the feed into the fermenter 100;
And an artificial rumen modeling device.
The method according to claim 1,
Wherein the fermenter (100) is hollowed to open the upper part, and a lid (300) for opening and closing the hollow interior is formed in the opened upper part.
3. The method of claim 2,
The lid 300 is provided with a stirrer 400 directly connected to a stirring rod 310 formed up to the inside of the fermentation tank 100. A buffer inlet 360 and a gas inlet 380 are provided on one end surface of the lid 300, ), a pH meter input port (350), a DO sensor input port (340), a thermometer input port (370), and a gas exhaust port (320).
The method according to claim 1,
The discharge port 200 is formed to be inclined upward in the diagonal direction, and an automatic cylinder brush device 230 is connected to an end portion of the discharge port 200. A discharge pipe 220, which branches in a lower diagonal direction, is protruded from one end of the discharge port 200 Wherein the artificial rheumatoid model device is characterized in that:
5. The method of claim 4,
Wherein the lower tube (220) of the outlet (200) is connected to a sample storage device (600) in which the overflowed rumen in the fermenter is transferred and stored.
6. The method of claim 5,
Wherein the sample storage device (600) is surrounded by a jacket (620) at an outer periphery, and cooling water is circulated inside the jacket (620) and connected to a constant temperature water tank for circulating the cooling water. Device.
The method of claim 3,
Wherein the buffer input port (360) is connected to a bottle (760) containing a buffer by a flow pipe.
The method of claim 3,
The gas exhaust port (320) discharges gas generated by fermentation in the fermentation tank (100), and the gas generated by the flowtube is connected to the tether bag and is collected in the tether bag.
The method according to claim 1,
In the fully automatic cylinder brush device 230,
A brush cylinder 250 detachably attached to an outlet 200 of the fermenter 100;
Is connected to one side of the brush cylinder 250 and is provided inside the discharge port 200 of the fermentation tank 100 and performs piston motion by the brush cylinder 250 to clean the inside of the discharge port 200 of the fermentation tank 100 Cleaning brush 240;
A motor 260 formed on the other side of the brush cylinder 250 to operate the brush cylinder 250 according to setting conditions;
And an artificial rumen modeling device.
The method according to claim 1,
The automatic feed input device (500)
A feed storage unit 510 vertically connected to the feed inlet 130 of the fermentation tank 100 and storing pelletized feed therein;
A feed inlet cylinder 520 connected to the feed inlet 130 of the fermenter 100;
The feed storage unit 510 is connected to one side of the feeding cylinder 520 and is disposed inside the feed inlet 130 of the fermentation tank 100. The feed stored in the feed storage unit 510 is positioned at the lower end of the feed storage unit 510, A feed hole 560 is formed in the feed hole 560 so that the feed hole 560 is moved by the feed feeding cylinder 520 to move the feed hole 560 to the inside of the fermentation tank 100. At the same time, (550) which is detached from the inside of the feeder (100);
A motor 530 formed on the other side of the feeding cylinder 520 to operate the feed feeding cylinder 520 in accordance with setting conditions;
And an artificial rumen modeling device.

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