KR20170112688A - Method for inducing artificial ovulation and spawn of eel - Google Patents
Method for inducing artificial ovulation and spawn of eel Download PDFInfo
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- KR20170112688A KR20170112688A KR1020160040116A KR20160040116A KR20170112688A KR 20170112688 A KR20170112688 A KR 20170112688A KR 1020160040116 A KR1020160040116 A KR 1020160040116A KR 20160040116 A KR20160040116 A KR 20160040116A KR 20170112688 A KR20170112688 A KR 20170112688A
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a method for inducing artificial ovulation and egg laying of freshwater eel, and more particularly, to a method for inducing artificial ovulation and egg laying in a freshwater eel, And to a method for inducing artificial ovulation of freshwater eel to induce an artificial ovulation under mixed water conditions.
Description
The present invention relates to a method for inducing artificial ovulation and egg laying of freshwater eel, and more particularly, to a method for inducing artificial ovulation and egg laying in a freshwater eel, And to a method for inducing artificial ovulation of freshwater eel to induce an artificial ovulation under mixed water conditions.
In the case of freshwater eel eels, the gonadotropins (GTHs) of the pituitary gland are insufficiently synthesized in the anthropogenic environment and the development of the gonads is not performed (Nagahama and Yamamoto, 1973) (Kumar et al., 1997; Ma et al., 2004), which is essential for the formation and development of vertebrate gonads.
Therefore, in the case of female freshwater eel, the abdominal injection of salmon pituitary extracts (SPE), which is a type of exogenous GTH, induces the completion of egg yolk formation and final maturation by 17α, 20β-dihydroxyprogesterone (DHP) Adachi et al., 2003), and repeatedly injected human chorionic gonadotropin (HCG) in male freshwater eels to induce the placement of human eosinophils (Yamamoto and Yamauchi, 1974; A study was also presented.
Here, referring to the results of artificial induction of ovulation of freshwater eels, SPE administration times differ according to the administration timing of SPE, and the results show that the difference in GTH responsiveness to ovaries and the difference (Fertilization rate, fertilization rate, and hatching rate).
In addition, when SPE is administered repeatedly to induce artificial ovulation in freshwater eels, the dose of each individual is excessively or insufficiently influenced, which in turn affects the ovulation that is artificially ovulated.
In addition, the freshwater eel is a submerged fish, while the salmon is a submerged fish. Freshwater eels move to seawater for scattering, while salmon migrate to freshwater for spawning, In the case of fish including freshwater eel, the pituitary extract (SPE) of salmon is used for promoting ovulation of fish including freshwater eel because species specificity is not so high. However, It is also necessary to study how to promote ovulation and scattering in a state where ovulation and scattering conditions approximate to natural environment while pituitary extract is applied.
SUMMARY OF THE INVENTION The present invention has been made in view of the circumstances of the prior art described above, and it is an object of the present invention to provide a stimulation-inducing substance (for example, 17 beta-Estradiol) for inducing magnetization over a certain period of time, When the induction period is reached, by mixing flathead mullet pituitary extracts (FPE), which is a descendant fish, as an artificial ovulation promoting substance in the aquaculture tank, the artificial ovulation can be promoted without applying the administration method by intraperitoneal injection And to provide a method for inducing artificial ovulation and egg laying of freshwater eel.
In order to achieve the above object, according to a preferred embodiment of the present invention, there is provided a method for inducing artificial ovulation and scattering of a freshwater eel in a culture aquarium, characterized in that conditions of fresh water, Inducing a magnetization inducing substance to induce magnetization and oviposition of the spermatozoa in the fresh water condition of the aquaculture water bath to induce magnetization, Wherein the osmotic pressure is adjusted by setting the water number of the water tank in the water tank condition so as to adjust the osmotic pressure of the water tank, Introducing an ovulation inducing substance collected from a fish; and a step of feeding the ovulation inducing substance, A method of inducing artificial ovulation and scattering of a freshwater eel is provided.
Also, according to the present invention, the magnetization inducing substance is 17 [beta] -estradiol.
Further, according to the present invention, in the step of inducing the autotrophy of the spermatozoa, the temperature of the aquaculture water is raised under a condition of gradually increasing from 20 ° C to 26 ° C.
Further, according to the present invention, the step of inducing the spermatogenesis of the spermatozoa is performed under the condition that the light is illuminated at a predetermined lighting period with the rise of the water temperature.
Further, according to the present invention, the lighting period is 8 to 10 hours.
Further, according to the present invention, the step of inducing the autonomousization of the siblings takes place for 3 to 6 weeks.
Further, according to the present invention, an ovulation inducing substance for inducing artificial ovulation is derived from a subtropical fish, which is a descending fish.
According to the present invention, in the step of controlling the osmotic pressure of the siblings, the sea water or salt water is supplied to the aquaculture water tank while the supply of the fresh water is stopped.
According to the present invention, in the step of inducing ovulation and natural scattering, the temperature of the culture water is gradually lowered.
According to the present invention, in the step of inducing ovulation induction and natural scattering, the temperature of the culture water is gradually decreased from 20 ± 0.5 ° C to 16-17 ° C.
In addition, according to the present invention, each step is carried out in the aquaculture apparatus, the aquaculture apparatus comprises a aqua regeneration tank, a warming unit in which a heater is installed to supply high-temperature aquaculture water to the aqua regeneration tank, A cooling unit installed in the aquaculture tank for periodically lighting the light, and a control unit for controlling temperature and light of the aquaculture water.
Further, according to the present invention, a light shielding film for blocking natural light is provided on the upper side of the aquarium, and the light source is disposed below the light shielding film.
Further, according to the present invention, the light source is irradiated with blue light having a wavelength of 400 to 500 nm.
Further, according to the present invention, the illuminance of the light source is 150 to 200 lux.
According to another aspect of the present invention, there is provided a control method of a temperature control apparatus, including a heater driving unit for driving a heater, an illumination driving unit for driving the light source, a water conditioning unit provided with a water temperature setting button and a light conditioning setting button, Wherein the temperature fluctuation period data and the light control data are provided by the water temperature setting button and the light condition setting button Respectively.
In addition, according to the present invention, a water temperature detection sensor is provided inside the aqua regia to detect the temperature of the aquaculture water and provide it to the control unit.
According to the artificial ovulation and scattering induction method of the freshwater eel according to the present invention, fresh water, nose and sea water conditions are established in the aquarium to induce sexual maturity and ovulation of the freshwater eel, and the irradiation period of the light is arbitrarily set Whereby the ovulation time can be shortened.
Further, in order to induce complete autotyping of the spermatozoa in the aquaculture tank, the autotrophy inducing substance is introduced as a pretreatment condition, then the osmotic control is performed via the base station, and then the seawater condition is set, The ovulation induction substance extracted from the mullet having a similar ecological migration characteristic to that of the freshwater eel, is introduced into the ovary, Deterioration can be suppressed to the utmost.
Brief Description of the Drawings Fig. 1 is a view showing a culture water tank for applying an ovulation induction method of a freshwater eel according to the present invention. Fig.
FIG. 2 is a view for explaining the morphological characteristics of egg development, which is referred to the explanation of artificial ovulation and scattering induction method of freshwater eel according to the present invention. FIG.
Hereinafter, the present invention will be described in detail.
First, according to a preferred embodiment of the present invention, a culture tank (fresh water) for breeding freshwater eel (for example, female) is immersed in a certain amount of a magnetization inducing substance (for example, 17β-Estradiol) Lt; / RTI > over a period of time.
In other words, in the case of freshwater eel culture, there are many cases of becoming male due to unclear reasons. Therefore, in order to secure the mother's female, In the aquaculture water, the magnetization inducing substance is mixed over a certain period (for example, 3 to 6 weeks).
On the other hand, according to the present invention, a magnetization inducing substance may be injected into the feed to feed the artificial ovulation induction subject.
In the pretreatment process, the mother's spermatozoa are kept in the condition of being kept in the condition of the wastewater condition for a certain period of time in a state in which the mother is secured. In this case, the seawater is gradually increased by 5 psu per day To be applied to seawater.
After the adaptation of the freshwater eel to the seawater as aquaculture water, the mullet pituitary extract (FPE) of the mullet is weighed at 20 to 120 mg per 1 kg of culture aquaculture It is mixed so that it gradually increases by date.
According to the present invention, in the process for inducing ovulation induction described above, the temperature of the culture water is reduced from 20 ± 0.5 ° C. to 16 to 17 ° C., and the salt concentration of the culture water is increased from fresh water to nadir To 35 psu so as to control the scattering conditions of the natural freshwater eel.
In the period during which the artificial inducing substance is introduced, the light source is provided in the culturing water tank corresponding to the artificial ovulation induction period so that the lighting period of the light source is, for example, 8 to 10 hours , It is preferable that the spontaneous scattering is induced by completely turning off during a period of 15 days in the switching process.
FIG. 1 is a culture water tank for applying the ovulation induction method of the freshwater eel according to the present invention. In the culture water tank, the condition of fresh water / navy water / sea water is electronically controlled and control of water temperature, Function.
In other words, referring to the drawings, '10' is a cultivated water tank which is made of transparent synthetic resin or a glass material and houses a fresh water eel. The lower end of the
The construction construction of '16' is configured to set a temperature fluctuation period of the water cultured in the
The control unit of '18' controls the overall form of the corresponding aquaculture system and controls the operation of the water conditioning system according to the temperature fluctuation period set by the water
The
A
The cooling unit of '28' performs a function of supplying the cultivated
The
According to the present invention, it is preferable to provide a water temperature detecting sensor for detecting the present temperature of the culture water and providing the water temperature sensor to the control unit.
The ovulation and scattering induction method of the freshwater eel according to the present invention applied to the above-described configuration will be described.
First, in the culture water tank, the number of the culture water is set to the fresh water condition by the water temperature setting button of the condition setting unit, the initial temperature is set to 20 DEG C, and the period during which the magnetization induction as the pre- And the lighting period of the light source is set to 10 hours so that the control by the control unit is performed. In the aquaculture water tank, the size of 30 to 40 cm (or 2.5 to 3 cm) Year-old) from the wild or cultured.
In this state, the culture water fed to the aquaculture tank is subjected to a pretreatment process for the magnetization by administering a magnetization inducing substance such as 17 beta-Estradiol or isoflavones for a predetermined period of time.
During the period during which the magnetization induction is performed, the mixed feed composition containing the above-described magnetization inducing substance may be fed to the culture tank two to five times a day.
Specifically, the mixed feed composition may include 100 parts by weight of fish meal, 1 to 10 parts by weight of a magnetization inducing substance, 1 to 5 parts by weight of a sulfurized powder, and 1 to 5 parts by weight of mandarin.
Since the curcumin contains curcumin as an active ingredient, the curcumin has a physiological activity such as an antioxidative effect and an anti-inflammatory effect, and thus promotes growth and magnetization of freshwater eel. When the content is less than 1 part by weight It is difficult to expect the above-mentioned physiological activity. If it exceeds 5 parts by weight, palatability may be deteriorated due to a unique flavor, so that it is preferable to limit the above-mentioned 1 to 5 parts by weight. Since the sulfurized powder is insoluble in water, it is preferable to further include 0.5 to 2 parts by weight of oligosaccharide to improve water solubility.
Mandarin acts to prevent microbial propagation and deterioration of the mixed feed composition through antioxidant action. Unlike the synthetic preservative used in fish, mandarin can minimize water pollution and genetic modification. When the amount is less than 5 parts by weight, it is difficult to sufficiently perform the antioxidative action described above, and if the amount exceeds 5 parts by weight, it is difficult to expect an increase in the effect. Therefore, the amount is preferably limited to 1 to 5 parts by weight.
And mandarins contain vitamin P and ascorbic acid, carotenoids, limonoid flavonoids, guarine, and naringenin ingredients. Among them, the Naringenin component is a component in the mandarin shell, which is an excellent antioxidant, and plays an important role in preventing the growth of microorganisms that come into contact with oxygen.
Meanwhile, an experiment was conducted to determine the growth rate increase by the mixed feed composition of the present invention.
For this experiment, a mixed feed composition (feed 1) containing 1 kg of squid fish meal, 15 g of 17 棺-Estradiol, 30 g of turmeric powder and 10 g of mandarin was placed in a water tank containing 100 kg of a test tube having an average length of 35 cm and a weight of 45 g Twice a day, 10 g / kg of feed was fed over 5 weeks. The feed composition (feed 2), which was prepared by mixing 1 kg of squid fish meal and 15 g of 17β-Estradiol, was fed into the control group of 34 cm in average body weight and 43 g of body weight over 5 weeks with 1 g of feed per 10 g of feed per day.
After the end of breeding by feed 1, the body size of the test group was 147 cm and the body weight was 285 g. After the end of breeding by the feed 2, the control body was grown to 127 cm in body weight and 185 g in body weight. It was confirmed from this experiment that the mixed feed composition of the present invention greatly contributes to the physiological activity and growth of the freshwater eel.
By the above-mentioned pretreatment process, the induction of the magnetization is confirmed in a known manner for the corresponding freshwater eel, and when the induction of the magnetization is completed, the process for ovulation and scattering proceeds.
That is, in the process for artificial ovulation and scattering, the water number setting mode is set by the water temperature setting button of the condition setting unit, and the initial temperature is set to 20 ° C and the lighting period of the light source is set to 8 to 9 hours So that the control by the control unit is executed.
Therefore, the control unit may supply the aquarium to the aquaculture tank for the aquaculture condition (in this case, supply the saline to the aquarium (or separately supply the saline water) to the aquaculture tank so that the saline is gradually supplied to the aquaculture water by 5 psu per day And is applied to seawater by an osmotic control process for about 7 days.
Then, the supply of fresh water to the aquaculture tank is stopped and the sea water condition is set so that the salt concentration is finally reached to 35 psu. In addition, the temperature of the aquaculture water is maintained at 20? The temperature is gradually raised from 0.5 DEG C to 16 DEG C to 17 DEG C so that the control is performed.
In addition to this, the culture water is mixed with 1 ~ kg of the culture water so that the FPE of 20 ~ 120 mg is gradually increased by the day according to the lighting period of the light source to induce ovulation, thereby inducing ovulation.
In addition, feeding of the feed is stopped while sufficient oxygen is supplied to the above-mentioned aquaculture tank.
For the FPE, an extract liquid homogenized with an Eel Ringer solution is applied to induce an artificial maturity, and a weight gain (weight gain = [(final body weight - initial body weight)] / initial body weight x 100) to determine maturity.
In the above-mentioned ovulation induction process, when the FPE is mixed with the aquaculture water and the embryo is visually ameliorated, the DHP (5-10 μg / g body weight concentration) or by injecting DHP with 2 μg / g body weight.
According to the present invention, the control unit controls so that the light source is turned off completely before and after the lighting period of the light source is on or off based on a report that the freshwater eel is scattered before and after the last day.
On the other hand, for male spermatozoa, high concentration of HCG (1000 IU / g body weight) is injected in accordance with the ovulation period of the female eel so that female and male are housed in the water tank at a ratio of 1: 2 to induce natural scattering and correction.
The spawning column is collected by piping the natural spawner and the fertilized egg to the aquaculture tank through the above-described process to induce the hatching in a state where a minute amount of oxygen is supplied from the hatching tank.
FIG. 2 is a view for explaining the morphological characteristics of egg development, which is referred to the explanation of artificial ovulation and scattering induction method of freshwater eel according to the present invention.
That is, 'A' in FIG. 2 represents the stage of egg yolk and embryogenesis of the freshwater eel. In the 1 hour after the modification, the division starts at the back of the animal pole, and 'B' (1.39 ± 0.07 mm) at the time point of passage of time, and 'C' progressed to the 16 cell stage at 3 hours after fertilization.
In addition, referring to 'D', when the fertilization egg is composed of 1 large oocyte and 2 ~ 3 small and medium oocyte at 5 hours after the modification, the state of progressing to the blastocyst is observed, At the end of the 14th hour of fertilization, it progresses to the middle part of the larvae, and 15 or more small petroglyphs are observed around the central large petri dish.
When 'F' is referred to, the embryo starts to form with the lapse of 21 hours, and after the lapse of 24 hours of correction, the embryo is fused into one, and 12 to 15 exfoliations are observed.
In addition, referring to 'G', anopharyngeal was formed at the passage of 27 hours of correction, and debridement was observed at the posterior part of the anopharynx. At 30 hours after correction, heart formation was observed in the lower part of the head, Blood vessels are observed, and the number of eradication is 24 ~ 30.
When referring to 'H', 28 to 35 exfoliations are observed when 33 hours have elapsed after the fertilization, and the tail is separated from the egg yolk in the form of a tail, and heart beating is observed. Referring to 'I' (Preleptocephalus) immediately after hatching is a little curved about 3 mm in total length, with 40 to 44 exterminations, and about one hour later, the body starts to hatch It is observed to be horizontal.
The present invention is not limited to the above-described embodiments, and various modifications and changes may be made without departing from the spirit and scope of the invention.
That is, a salt water tank may be separately added to the aquaculture tank so that the salt water is supplied to the aquaculture water tank under the control of the control unit.
10: Aquaculture tank 14: Sunshield
16: Joe construction 18: Control
20: control data storage unit 22:
24: heating section 26: heater driving section
28: cooling section 30: light source
Claims (2)
Controlling the conditions of the fresh water, the nose water, and the seawater to be sequentially set in the aquarium;
Inducing magnetization by injecting a magnetization inducing substance for inducing magnetization and oviposition of the spermatozoa in the fresh water condition of the aquaculture tank,
Setting the number of the aquarium of the aquarium to be an atmospheric condition after the magnetization induction step is performed,
Introducing the ovulation inducing material collected from the descending fish to induce ovulation of the sperm while setting the number of aquaculture of the aquaculture tank to seawater condition after the osmotic control step is executed,
Inducing natural spawning after the step of injecting the ovulation inducing substance,
In the step of inducing the autotrophy, the mixed feed composition containing the magnetization inducing substance is put into the aquaculture tank for breeding,
Wherein the mixed feed composition comprises 100 parts by weight of fish meal, 1 to 10 parts by weight of a magnetization inducing substance, 1 to 5 parts by weight of a sulfurized powder, and 1 to 5 parts by weight of mandarin. Induction method.
Wherein the magnetization inducing substance is 17 [beta] -estradiol,
In the step of inducing the autotrophy of the spermatozoa, the temperature of the culture water is set to be gradually increased from 20 ° C to 26 ° C,
The step of inducing the autotrophy of the spermatozoa is carried out under the condition that the light is turned on at a predetermined lighting period with the rise of the water temperature,
Wherein the lighting period is 8 to 10 hours. ≪ RTI ID = 0.0 > 8. < / RTI >
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108056050A (en) * | 2017-12-22 | 2018-05-22 | 钦州市钦州港国鸿水产养殖专业合作社 | A kind of turbot aquaculture method |
| WO2019225990A1 (en) * | 2018-05-23 | 2019-11-28 | 제이제이앤구골홀딩스 주식회사 | Integrated management platform for artificial seedling and raising of anguilla japonica |
| KR20200132240A (en) * | 2019-05-16 | 2020-11-25 | 대한민국(관리부서:국립수산과학원) | The method of sex determination of Anguilla japonica by measurement of hormone in blood |
| CN113475432A (en) * | 2021-07-26 | 2021-10-08 | 董俊武 | Domestication method of wild fries in finless eel culture |
| KR102563229B1 (en) * | 2022-02-16 | 2023-08-03 | 이장원 | Seawater transfer regime of steelhead trout for improving hypoosmoregulatory adaptation, feed efficiency, and growth performance |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000139361A (en) * | 1998-11-02 | 2000-05-23 | Hayashikane Sangyo Kk | Antibacterial feed for culturing fish |
| JP2008154459A (en) | 2006-12-20 | 2008-07-10 | Irago Institute Co Ltd | Method for accelerating maturation of eel |
| CN102511424A (en) | 2011-12-23 | 2012-06-27 | 上海海洋大学 | Artificial eel propagation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108056050A (en) * | 2017-12-22 | 2018-05-22 | 钦州市钦州港国鸿水产养殖专业合作社 | A kind of turbot aquaculture method |
| WO2019225990A1 (en) * | 2018-05-23 | 2019-11-28 | 제이제이앤구골홀딩스 주식회사 | Integrated management platform for artificial seedling and raising of anguilla japonica |
| KR20200132240A (en) * | 2019-05-16 | 2020-11-25 | 대한민국(관리부서:국립수산과학원) | The method of sex determination of Anguilla japonica by measurement of hormone in blood |
| CN113475432A (en) * | 2021-07-26 | 2021-10-08 | 董俊武 | Domestication method of wild fries in finless eel culture |
| CN113475432B (en) * | 2021-07-26 | 2024-01-05 | 董俊武 | Domestication method of wild fries in finless eel culture |
| KR102563229B1 (en) * | 2022-02-16 | 2023-08-03 | 이장원 | Seawater transfer regime of steelhead trout for improving hypoosmoregulatory adaptation, feed efficiency, and growth performance |
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