KR20170109362A - Kit and Method for Detecting Bacteria - Google Patents
Kit and Method for Detecting Bacteria Download PDFInfo
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- KR20170109362A KR20170109362A KR1020160033384A KR20160033384A KR20170109362A KR 20170109362 A KR20170109362 A KR 20170109362A KR 1020160033384 A KR1020160033384 A KR 1020160033384A KR 20160033384 A KR20160033384 A KR 20160033384A KR 20170109362 A KR20170109362 A KR 20170109362A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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Abstract
The present invention relates to a kit for easily and easily detecting bacteria and a method thereof. The present invention relates to a method of manufacturing a microfluidic device, which comprises a main body 110 formed in a cylindrical shape and made of glass, transparent or translucent plastic material and containing therein a selective medium for propagation and detection of bacteria; A specimen collection unit 120 for collecting harmful bacteria and immersing the collected bacteria in the selective medium M; The one end of the specimen collection unit 120 is fixed and the specimen collection unit 120 keeps the specimen collection unit 120 spaced apart from the selective medium in a state in which the specimen collection unit 120 does not collect the specimen, And a selection control unit (130) for allowing the specimen collection unit (120) to be immersed in the selective medium when the specimen collection unit (120) collects the specimen. When the present invention is used, it is possible to determine whether a specific bacterium is infected within a very short period of time, and there is an advantage in that a health authority can quickly carry out a subsequent administrative procedure.
Description
The present invention relates to a kit for easily and easily detecting bacteria and a method thereof. More particularly, the present invention relates to a kit for identifying and identifying a person who holds human harmful bacteria from a large number of people to be tested To a bacterial detection kit capable of detecting a target bacteria safely and easily, and a method of detecting bacteria using the kit.
In today 's society, a lot of individuals gather together to perform public activities. These public activities often have a direct impact on others in the community, depending on the health of the individual or the infection of the bacterium. Particularly, when a member of a social community is infected with a contagious bacterium, emergency measures such as spreading various preventive activities or isolating infected patients are conducted in order to prevent the spread of bacterial infection.
In today 's society, it is very important to judge whether or not the bacteria are harmful to human body. Among them, there are a lot of factors related to the eating habits enjoyed by the general public. This diet is becoming more and more important as the food culture is rapidly changing due to the increase in the consumption of food and drink, and the consumption of processed food. In the case of processed food, the HACCP system has been introduced and gradually strengthened due to the increased social interest in food hygiene and safety. On the other hand, as the food delivery business of the general public, order delivery business of lunch boxes, In the case of facilities that supply large quantities of food, issues related to food poisoning are emerging as social problems.
Food poisoning is caused by food poisoning by natural poison (poisonous poison, mushroom poison, etc. Food poisoning by food corruption; And food poisoning due to the inclusion of bacteria in food. However, the most common problem in the industry for the general public is that bacteria are mixed into foods. The contamination or propagation with the food by the bacteria is not generally observed by the naked eye. Once the food poisoning occurs in the facility that supplies the large amount of foods, it affects a considerable number of people. Therefore, It is important to take appropriate preventive measures such as discovery and disinfection early and thoroughly prevent it.
In order to prevent food poisoning, health authorities and hospitals are conducting administrative procedures to examine and inspect whether or not they are infecting food-borne causative organisms every year in the catering industry. This procedure is carried out by incubating specimens collected from foodservice workers on an agar plate using an enrichment medium and a selective medium, and observing the colonies formed on the medium by visual inspection to identify suspected colonies through various identification steps . Recently, polymerase chain reaction (PCR) has been used to determine whether food poisoning bacteria are infected.
However, all of these conventional methods are troublesome to operate and require expertise and skill for detection in order to determine colonies grown on the agar medium. In the case of PCR, Or skills and special equipment.
In addition, such a conventional method has a drawback that the inspection period is too long. First of all, it takes a long time to cultivate and propagate bacteria from a specimen and it takes 7 to 10 days to determine the final infection with food poisoning bacteria. During such an inspection period, workers at the catering establishments who are under inspection are not able to accurately determine whether the bacteria are infected, so that they can not only infect a large number of infected persons but also engage in related work, . In addition, even in the case of administrative agencies such as the health departments of health, it is necessary to carry out related duties continuously for a long period of time.
In order to improve the conventional method as described above, several bacterial detection mechanisms have emerged. However, these bacterial detection mechanisms and the like have a very complicated external structure and a very complicated internal structure, There is a disadvantage that it is cumbersome for the general public to use it effectively.
Prior art related to the conventional bacteria detection method and kit are as follows.
Disclosure of Invention Technical Problem [8] The present invention has been made to solve all the problems of the prior art described above, and it is an object of the present invention to provide a method and apparatus for identifying a person having harmful bacteria from a large number of people, The present invention provides a kit for detecting bacteria.
It is another object of the present invention to provide a method for rapidly detecting harmful bacteria by using a safe and simple detection kit of the bacteria.
In order to achieve the above-mentioned object, the present invention provides a method for producing a microorganism, which comprises a main body having a cylindrical shape and formed of glass, transparent or semitransparent plastic material and containing therein a selective medium for propagation and detection of bacteria; A specimen collecting unit for collecting harmful bacteria and for inoculating the collected specimens into the selective medium (M); Wherein the sample collection unit is held at a position spaced apart from the selection medium in a state in which one end of the sample collection unit is fixed and the sample collection unit does not collect the sample, and when the sample collection unit is collected, A selective regeneration unit which is immersed in the selective medium for inoculation; And a kit for detecting a harmful germ of a human body.
The main body portion is sealed in a rounded manner at its lower end, and has a selective medium accommodating portion containing the selective medium M therein, a central space portion having a center at a center thereof, It is preferable that an upper opening is formed.
The main body may be formed as a two-step separable type in which the selected-medium accommodating portion and the upper opening portion are detachable and detachable.
It is preferable that the sample collecting part has a long rod shape and includes a rod rod whose one end is detachably coupled to the selection control part and a sample collecting rod formed at the other end of the rod and capable of collecting the sample .
Preferably, the selection regulating unit includes a first stopper for coupling one end of the rod of the sample collecting unit and sealing the upper opening of the main body, and a second stopper provided above the first stopper in a state connected to the first stopper Do. It is preferable that a distance L 1 between the first stopper and the second stopper is longer than a distance L 2 between the selection medium M and the sample collecting rod.
The present invention also provides a method of analyzing a sample, comprising: separating the selection controller from a main body and collecting a sample from a subject using the sample collecting rod; The specimen collection rod taken in the specimen is inserted into the main body portion and force is applied so that the first stopper enters the inside of the main body portion and the second stopper comes in close contact with the end of the opening portion of the main body portion, So that the sample collecting rod is fully immersed in the selection medium of the main body part and inoculated; Culturing the selective medium of the body part in a 37 ° C incubator for 24 hours to 36 hours and discriminating specific bacteria by changing the color of the selective medium; .
When the kit according to the present invention is used, it is possible to confirm whether a specific bacterium is infected within 24 to 36 hours.
In addition, when the kit according to the invention is used, it is possible to quickly determine whether a particular bacterium is infected, so that the administrative agency can perform rapid follow-up on it, and a general person such as a foodservice worker, There is also an advantage of confirming the result.
In addition, when the kit according to the invention is used, since the selective medium in the kit is not a fluid having a fluidity as in the conventional art, the culture medium infected with the bacteria by the user's fruit flows out to the outside, There is also an advantage that there is no fear of leakage.
In addition, when the kit according to the present invention is used, since it can be partially recycled, saving of resources and prevention of environmental pollution can be achieved at the same time.
1 is a conceptual diagram of a kit for detecting bacteria according to the present invention,
FIG. 2 is a conceptual view showing a state of using a kit for detecting bacteria according to the present invention,
FIG. 3 is a conceptual diagram showing a decomposition state of a kit for detecting bacteria according to the present invention, which is a preferred embodiment in which the body part is divided into two parts,
FIG. 4A is a preferred embodiment showing the relationship between the sample collecting part and the selection controlling part of the present invention,
FIG. 4B is another preferred embodiment showing the relationship between the sample collecting part and the selection controlling part of the present invention,
5 is an enlarged cross-sectional view showing a coupling relationship between the main body part and the selection control part of the present invention.
Hereinafter, the present invention will be described in more detail and in detail. It is apparent that the present invention is not limited thereto and that the present invention is not limited thereto. In the description of the present invention, the same reference numerals are used for the same parts, and parts that can be easily created by those having ordinary skill in the art are omitted in the description of the present invention .
The present invention provides a kit (100) for detecting a bacterium which can identify a specific bacterium safely and quickly.
The
The
The
It is preferable that the selective medium (M) has non-flowability at room temperature. When the selective medium M is non-ferrous at room temperature, the
Further, it is preferable that the human harmful bacteria suitable for the selective medium (M) are food poisoning bacteria. Examples of the food poisoning bacteria include Salmonella Thyphi , Salmonella spp ., Shigelladysentriae , Staphylococcus aureus , Escherichia Coli And the like.
In the present invention, it is preferable that the
In the present invention, it is preferable that the
The
The
The
The
The
The
The
The principle is as follows.
1, the
On the other hand, when the user inserts the
Further, the present invention provides a method for quickly determining whether or not a bacterium is infected using the
The present invention includes a step of separating the
The sample collection step is the first step performed using the
The present invention includes a step of inoculating and culturing a
In the step of inoculating and culturing the sample in the selective medium, the
When this condition is reached, the
The present invention is characterized in that the selective medium M of the
Hereinafter, the present invention will be described as more specific examples.
One). Selection medium (M):
The selective medium (M) used in the present invention was a chromogenic medium produced by the applicant. The components of the selective medium (M) are shown in Table 1 below.
The components contained in the selective medium M include a nutrient component capable of growing microorganisms and provide a stable environment for the microbial growth of the microorganisms present in the specimen by the added nutrients, indicators, coloring agents, and the like Therefore, the microorganisms can be prevented from drying due to internal components and moisture of the medium even during transportation for confirmation inspection, and viability can be maintained. The selective medium was inserted into the selective medium
2). Strain and sample
The strains were Salmonella Thyphi , Salmonella spp ., Shigelladysentriae , Staphylococcus aureus, Escherichia Coli , Klebsiellaspp ., Enterobacter spp . Citrobacterspp. Were diluted to a constant concentration and used.
3). Experimental Method
In order to confirm the color change according to the species in the selective medium, the results were observed using the strain. The concentration of the strain was diluted using a sterilized PBS buffer with a 0.5 McFarland standard solution as a control, and then inoculated with a sterilized
4). Experiment result
The experiment was carried out by inoculating 5 isolates of each of 8 strains into the selective medium (M) of different serial number 10 times. As a result, the chromagens corresponding to the strains inoculated into the chrome agar plates within 24 to 36 hours in all cases there was.
In particular, Salmonella spp . Group, Shigelladysentriae , and Staphylococcus aureus showed magenta (P) and other intestinal bacteria showed blue (B) color. (See Fig. 6)
Thus, when a specimen is obtained from those who are engaged in the public or entertainment establishments, and the specimen is inoculated into the selective medium according to Table 1, when magenta (P) , The person can be identified as a person infected with food poisoning bacteria.
For these people, government agencies such as public health centers judge them as carriers of food poisoning bacteria, and after that, they are allowed to go through prescribed administrative procedures. On the other hand, medical institutions such as hospitals conduct more precise inspections as foodborne pathogens, We will proceed to treatment of infection.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, The range is determined and limited.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the present invention.
100: kit for detecting bacteria (present invention)
110: main body portion, 112: selective medium accommodating portion,
114: central clearance, 116: upper opening,
118: protrusion, 120: specimen collection part,
122: rod rod, 124: sample collecting rod,
130: selection control unit, 132: first stopper,
134: second stopper, 136: engaging groove
Claims (8)
A main body 110 formed in a cylindrical shape and made of glass, transparent or translucent plastic material and containing a selective medium for propagation and detection of bacteria therein;
A specimen collection unit 120 for collecting harmful bacteria and immersing the collected specimen in the selective medium M;
The one end of the specimen collection unit 120 is fixed and the specimen collection unit 120 keeps the specimen collection unit 120 spaced apart from the selective medium in a state in which the specimen collection unit 120 does not collect the specimen, And a selection control unit 130 for allowing the specimen collection unit 120 to enter the selected medium and to be inoculated when the specimen collection unit 120 collects the specimen
(100) for detecting human harmful bacteria.
The main body 110 is sealed at its lower end and has a selective medium accommodating portion 112 accommodating the selective medium M therein and a central space portion 114 and an upper opening 116 whose upper end is completely opened,
The sample collecting unit 120 has a long rod shape and includes a rod rod 122 whose one end is detachably coupled to the selection and control unit 130 and a sample rod 122 formed at the other end of the rod rod 122, And a sample collecting rod 124 capable of collecting the sample,
The selection controller 130 includes a first stopper 132 for coupling one end of the rod 122 of the sample collecting unit and sealing the upper opening 116 of the body 110, , And a second stopper (134) located above the first stopper (132) in a state connected to the second stopper (132).
The selection and control unit 130 controls the distance L 1 between the first stopper 132 and the second stopper 132 to a distance L 1 between the selective medium M and the sample- Is longer than the distance (L 2 ) between the electrodes (1) and (2).
Wherein the main body part (110) is formed as a two-step separation type in which the selective medium accommodating part (112) and the upper opening part (116) (100).
The kit (100) for detecting human harmful bacteria, wherein the human harmful bacteria are food poisoning bacteria.
A kit for detecting human harmful bacteria according to claim 1,
Separating the selection controller 130 from the main body 110 and sampling the specimen from the examinee using the specimen collecting rod 124;
The sample collecting rod 124 is inserted into the main body 110 and force is applied to insert the first stopper 132 into the main body 110 and the second stopper 134, So that the sample collecting rod 124 completely wets into the selective medium M of the main body 110 and inoculates the specimen by making the sample collecting rod 124 in close contact with the opening 116 of the main body 110 ;
Culturing the selective medium (M) of the main body (110) for 24 hours to 36 hours and discriminating specific bacteria through color change of the selective medium (M); To
And detecting the harmful bacteria in the human body.
The human harmful bacteria are food poisoning bacteria,
Wherein the selective medium (M) is a coloring medium.
Wherein the selective medium (M) is non-flowable at room temperature.
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KR1020160033384A KR101861266B1 (en) | 2016-03-21 | 2016-03-21 | Method for Determining Whether a Person May Be Infected by Food Poisoning Bacteria or Not |
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KR1020160033384A KR101861266B1 (en) | 2016-03-21 | 2016-03-21 | Method for Determining Whether a Person May Be Infected by Food Poisoning Bacteria or Not |
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