KR100316321B1 - Kit for detecting microorganisms - Google Patents

Abstract

PURPOSE: A kit for inspecting microorganisms is provided, thereby easily and rapidly inspecting microorganisms, particularly, bacteria causing food-poisoning. CONSTITUTION: The kit for inspecting microorganisms is composed of a transparent vessel having a hand shaped surface or a hand shape design in an oval; a transparent cover with the same material as the vessel; and a selective medium being coated on the bottom of the vessel to inspect the presence of bacteria when a person imprints the palm on the hand shaped selective medium, in which the selective medium contains 7.0 to 9.0 wt.% of NaCl, 0.7 to 1.0 wt.% of D-mannitol, and 0.001 to 0.003 wt.% of phenol red, or alternatively contain 7.0 to 1.5 wt.% of NaCl, 0.7 to 1.2 wt.% of lactose, 0.1 to 0.3 wt.% of bile salts, 0.001 to 0.005 wt.% of neutral red, 0.00005 to 0.00002 wt.% of crystal violet, and 1.4 to 2.4 wt.% of agar. The selective medium may further contain 0.05 to 6 wt.% of polyacrylamide.

Description

Kit for detecting microorganisms

The present invention relates to a kit for testing microorganisms, and more particularly, by culturing microorganisms contaminated or adhered to a palm in a special medium, the type and number of microorganisms can be changed by changing the color of the medium according to the growth of contaminated bacteria. It relates to a microbiological test kit consisting of a container and a lid containing a selection medium that can be easily identified.

Food poisoning accidents due to unsanitary treatment of food are increasing at home and abroad, and food poisoning accidents caused by microorganisms in the hands of food handlers account for 20% of the total. Therefore, if the food handler's hand can be easily inspected from time to time before handling the food, the secondary contamination of the food by the food handler's hand can be prevented in advance and the food handler's hygiene concept can be improved.

There are a large number of microorganisms attached to or grown on the hands, and microorganisms causing food poisoning are mainly staphylococcus, Clostridium, Salmonella, etc., but the type that can be attached or grown under the environment of the hand is staphylococcus. These food poisoning-inducing microorganisms are grown after being attached to or attached to the hands of food handlers by contacting food handlers with food or objects already contaminated with the microorganisms. Diarrhea, vomiting, convulsions will cause so-called food poisoning symptoms. In addition, E. coli or E. coli usually tend to be used as an indicator of the overall bacterial contamination.

Here, E. coli group refers to E. coli, which belongs to E. coli, but has not yet been identified as a microorganism with slightly different characteristics. E. coli bacteria are particularly mutated, so it is not easy to identify them completely. However, E. coli and E. coli are infected by similar pollutants, require a similar growth environment, and produce similar products. Therefore, in the present specification, E. coli and E. coli group are bundled and handled.

Conventionally, in order to examine the hands of food handlers, using a cotton swab to collect the bacteria from a certain area (5cm X 5cm) of the hand, put a cotton swab in sterile distilled water and suspended using a vibrator and inoculate a certain amount into a normal medium After incubation, colonies of the cultured microorganisms were purely separated and subjected to biochemical and serological tests to finally determine whether the food poisoning microorganisms.

However, such a conventional method is difficult to target the whole hand, and also requires equipment such as swabs, sterile distilled water, suspension vibrators, micro pipettes, biochemical and serological test reagents, and the like. There was a disadvantage in that it needed the help of the equipped test personnel and finally took 3-5 days to determine food poisoning microorganisms. Therefore, since the method has a lot of difficulties in the field inspection, only the sampling is performed in the field, and most of the subsequent processes are transferred to the laboratory for inspection.

The present invention has been made to solve the problems of the prior art as described above, in the kit prepared by dispensing a high-selectivity food poisoning microorganisms test medium prepared in advance in a container made of a plastic material in the palm of the preparation without additional preparation at the food production site The object of the present invention is to provide a food poisoning microorganism testing kit for hand to easily test the food poisoning microorganisms present in the hand through the color of the colony after contacting the test medium on the culture.

1 is a plan view of an example of a container according to the present invention (lattice and microlattice show only a portion).

Figure 2 is a plan view of another example of a container according to the present invention (lattice and microlattice show only a portion).

In order to achieve the above object, the present invention, there is provided a container and a lid corresponding to the container, the bottom of the container provides a kit for testing microorganisms coated with a selective medium.

The microbial examination kit may have a round shape, a quadrangular shape or a polygon shape, a palm shape or an oval shape, and a palm shape. The material may be any material capable of preventing infection by an undesired microorganism during transport, culture, and use of the microbiological test kit such as PET, plastic, glass, aluminum, or stainless steel.

Square lattice and microlattices may be made inside or outside the bottom of the vessel. The length of one side of the grating is 5 ~ 20mm, the microlattice can be produced in a variety of 0.5 ~ 3mm.

In the present invention, a selective medium may be used to selectively grow Staphylococcus and E. coli or E. coli groups. In order to test for staphylococcus, the medium in the present invention contains 7.0 to 9.0% by weight of salt (NaCl), 0.7 to 1.0% by weight of D-Mannitol, and 0.001 to 0.003% by weight of phenol red. do. In addition, the medium in the present invention in order to test for E. coli or E. coli group salt (NaCl) 0.7 ~ 1.5% by weight, lactose (Lactose) 0.7 ~ 1.2% by weight, Bil salts (Bile salts) 0.1 ~ 0.3% by weight, neutral red (Neutral Red) 0.001 to 0.005% by weight, Crystal violet (Crystal violet) 0.00005 to 0.0002% by weight, Agar (Agar) includes 1.4 to 2.4% by weight. It is also possible to add general nutrients necessary for the growth of other microorganisms.

In the medium, salt acts to selectively inhibit non-food-addictive microorganisms with low salinity resistance, and as a carbon source, D-mannitol and lactose, respectively, function to grow only staphylococci and Escherichia coli that can magnetize them. do. For E. coli, additional salt is added to increase selectivity. In the selective medium of the present invention, a pancreatic digest of casein, peptic digest of animal tissue, beef extract, etc., to provide nutrients other than a carbon source, An appropriate amount of agar is added to solidify and ensure strength.

Staphylococcus and E. coli magnetize the carbon source to produce organic acids, so that the indicators, phenol red, neutral red and crystal violet, change color according to the pH change of the medium, thereby confirming the presence of the bacteria.

On the other hand, in the present invention, in order to increase the strength of the selective medium, 0.05 to 6% by weight of polyacrylamide may be added.

Hereinafter, the configuration of the present invention will be described in detail by quoting an embodiment.

Example 1

NaCl 90g, D-Mannitol 10.0g, Pancreatic digest of casein 5.0g, Peptic digest of animal tissue 5.0g, Beef extract 1.0g Phenol Red 0.025g, Agar 15.0g are dissolved in 1 liter of distilled water and the pH is set to 7.4 (25). After fitting, sterilization was performed at 121 ° C. for 15 minutes using autoclave. After 40 minutes in the kit as shown in Figure 1 and left for 1 hour at room temperature.

Example 2

NaCl 100g, D-Mannitol 10.0g, Pancreatic digest of casein 5.0g, Peptic digest of animal tissue 5.0g, Beef extract 1.0g Phenol Red 0.025g, Agar 15.0g, 1.3g polyacrylamide in 1 liter of distilled water To 7.4 (25) and sterilized for 15 minutes at 121 ℃ by autoclave. Thereafter, 40 ml was dispensed into the kit as shown in FIG. 1 and left at room temperature for 1 hour.

Example 3

NaCl 110g, D-Mannitol 10.0g, Pancreatic digest of casein 5.0g, Peptic digest of animal tissue 5.0g, Beef extract 1.0g Phenol Red 0.025g, Agar 25.0g, Polyacrylamide 6g in 1 liter of distilled water 7.4 (25) was then sterilized for 15 minutes at 121 ℃ by autoclave. Thereafter, 40 ml was dispensed into the kit as shown in FIG. 1 and left at room temperature for 1 hour.

Example 4

NaCl 10.0g, Pancreatic digest of gelatin 17.0g, Lactose 10.0g, Bile salts 2.0g, Pancreatic digest of casein 1.5g Peptic digest of animal tissue 1.5g, Neutral Red 0.03g, Crystal violet 1.0mg, Agar 15.0g, Polyacrylic After dissolving 4.0 g of amide in 1 liter of distilled water, the pH was adjusted to 7.1 (25) and sterilized at 121 ° C. for 15 minutes using an autoclave. Thereafter, 40 ml was dispensed into the kit as shown in FIG. 1 and left at room temperature for 1 hour.

Example 5

NaCl 20.0g, Pancreatic digest of gelatin 17.0g, Lactose 10.0g, Bile salts 2.0g, Pancreatic digest of casein 1.5g, Peptic digest of animal tissue 1.5g, Neutral Red 0.03g, Crystal violet 1.0mg, Agar 15.0g, Poly 2.5 g of acrylamide was dissolved in 1 liter of distilled water, and then the pH was adjusted to 7.1 (25), followed by sterilization at 121 ° C. for 15 minutes using an autoclave. Thereafter, 40 ml was dispensed into the kit as shown in FIG. 1, and then left at room temperature for 1 hour.

Application example

Thirty food store workers were selected and their hands were brought into contact with the test kit prepared in Example 1-5 for 3 seconds, and the kit was examined after 18-24 hours, according to the number of microorganisms detected in the medium. The result was obtained.

The number of colonies detected Example 1 Example 2 Example 3 Example 4 Example 5 0 21 people 25 persons 23 persons 15 people 17 people 1-10 4 people 2 people 3 people 7 people 6 people 11-100 3 people 1 person 3 people 4 people 4 people 101 or more 2 people 1 person 1 person 4 people 3 people

* Example 1-3 is yellow colony, Example 4-5 is pink colony

150 yellow colonies and 150 pink colonies detected in Application Example 1 were purely separated and subjected to biochemical and serological tests. As a result, 150 yellow colonies were identified as staphylococci, food poisoning microorganisms, and 97 pink colonies were E. coli and the rest. 53 were identified as E. coli.

The present invention made up of the above objects and configurations is prepared by dispensing a high selectivity food poisoning microorganism test medium prepared in advance in the hand-shaped PET material plate for 3 seconds without a separate preparation at the food production site The food poisoning microorganism test can be carried out quickly by contacting, and the test time can be shortened.

Since the food poisoning microorganisms existing in the hands of the workers are conventionally performed by a specialist in the laboratory, it is difficult for food handlers to directly read the results, but the food poisoning microorganisms can be distinguished by color, so that the food poisoning microorganisms can be read in the field.

In addition, by directly observing food poisoning microorganisms contaminated in the hands of food handlers can be expected to prevent food hazards in advance by improving the hygiene concept of food handlers.

Claims (5)

There is a transparent container made of a flat hand shape or a palm-shaped shape inside an oval, and a transparent lid corresponding to the container, and a selective medium is coated on the inner bottom of the container so that the user directly The microbiological test kit, characterized in that by immediately taking the palm of the culture medium to determine whether or not the selective bacteria present in the medium of the user's hand. The method of claim 1, The selective medium is 7.0 to 9.0 wt% of salt (NaCl), 0.7 to 1.0 wt% of D-Mannitol, 0.001 to 0.003 wt% of phenol red (Phenol Red) for the test Kit. The method of claim 1, The selective medium is 0.7 to 1.5% by weight of salt (NaCl), 0.7 to 1.2% by weight of lactose (Lactose), 0.1 to 0.3% by weight of salt salt (Neutral Red) 0.001 to 0.005% by weight, crystal Violet (Crystal violet) 0.00005 ~ 0.0002% by weight, agar (Agar) kit for microorganisms, characterized in that it comprises 1.4 to 2.4% by weight. The method according to claim 2 or 3, The selective medium is a microbial test kit, characterized in that it further comprises 0.05 to 6% by weight of polyacrylamide. The method according to any one of claims 1 to 3, The bottom of the container is divided into a lattice and a fine lattice microbiological test kit, characterized in that to easily count the number of microorganisms cultured on the medium.