KR101861266B1 - Method for Determining Whether a Person May Be Infected by Food Poisoning Bacteria or Not - Google Patents

Method for Determining Whether a Person May Be Infected by Food Poisoning Bacteria or Not Download PDF

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KR101861266B1
KR101861266B1 KR1020160033384A KR20160033384A KR101861266B1 KR 101861266 B1 KR101861266 B1 KR 101861266B1 KR 1020160033384 A KR1020160033384 A KR 1020160033384A KR 20160033384 A KR20160033384 A KR 20160033384A KR 101861266 B1 KR101861266 B1 KR 101861266B1
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selective medium
rod
bacteria
collection unit
sample
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KR1020160033384A
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KR20170109362A (en
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안영기
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주식회사 아이앤이
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

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Abstract

The present invention relates to a kit for easily and easily detecting bacteria and a method thereof. The present invention relates to a method of manufacturing a microfluidic device, which comprises a main body 110 formed in a cylindrical shape and made of glass, transparent or translucent plastic material and containing therein a selective medium for propagation and detection of bacteria; A specimen collection unit 120 for collecting harmful bacteria and immersing the collected bacteria in the selective medium M; The one end of the specimen collection unit 120 is fixed and the specimen collection unit 120 keeps the specimen collection unit 120 spaced apart from the selective medium in a state in which the specimen collection unit 120 does not collect the specimen, And a selection control unit (130) for allowing the specimen collection unit (120) to be immersed in the selective medium when the specimen collection unit (120) collects the specimen. When the present invention is used, it is possible to determine whether a specific bacterium is infected within a very short period of time, and there is an advantage in that a health authority can quickly carry out a subsequent administrative procedure.

Description

Method for Determining Whether a Food Poisoning Bacterium Is Infected [

The present invention relates to a method for determining whether a food poisoning microorganism has been infected or not, and more particularly, to a method for identifying a food poisoning microorganism belonging to human harmful bacteria from a large number of people And a method for determining whether a food poisoning bacterium is infected by a large number of people safely and easily.

In today 's society, a lot of individuals gather together to perform public activities. These public activities often have a direct impact on others in the community, depending on the health of the individual or the infection of the bacterium. Particularly, when a member of a social community is infected with a contagious bacterium, emergency measures such as spreading various preventive activities or isolating infected patients are conducted in order to prevent the spread of bacterial infection.

In today 's society, it is very important to judge whether or not the bacteria are harmful to human body. Among them, there are a lot of factors related to the eating habits enjoyed by the general public. This diet is becoming more and more important as the food culture is rapidly changing due to the increase in the consumption of food and drink, and the consumption of processed food. In the case of processed food, the HACCP system has been introduced and gradually strengthened due to the increased social interest in food hygiene and safety. On the other hand, as the food delivery business of the general public, order delivery business of lunch boxes, In the case of facilities that supply large quantities of food, issues related to food poisoning are emerging as social problems.

Food poisoning is caused by food poisoning by natural poison (poisonous poison, mushroom poison, etc. Food poisoning by food corruption; And food poisoning due to the inclusion of bacteria in food. However, the most common problem in the industry for the general public is that bacteria are mixed into foods. The contamination or propagation of food by the bacteria is generally not observed by the naked eye. Once food poisoning occurs in a facility that supplies a large amount of food, it affects a considerable number of people. Therefore, It is important to take appropriate preventive measures such as discovery and disinfection early and thoroughly prevent it.

In order to prevent food poisoning, health authorities and hospitals are conducting administrative procedures to examine and inspect whether or not they are infecting food-borne causative organisms every year in the catering industry. This procedure is carried out by incubating specimens collected from foodservice workers on an agar plate using an enrichment medium and a selective medium, and observing the colonies formed on the medium by visual inspection to identify suspected colonies through various identification steps . Recently, polymerase chain reaction (PCR) has been used to determine whether food poisoning bacteria are infected.

However, all of these conventional methods are troublesome to operate and require expertise and skill for detection in order to determine colonies grown on the agar medium. In the case of PCR, Or skills and special equipment.

In addition, such a conventional method has a drawback that the inspection period is too long. First of all, it takes a long time to cultivate and propagate bacteria from a specimen and it takes 7 to 10 days to determine the final infection with food poisoning bacteria. During such an inspection period, workers at the catering establishments who are under inspection are not able to accurately determine whether the bacteria are infected, so that they can not only infect a large number of infected persons but also engage in related work, . In addition, even in the case of administrative agencies such as the health departments of health, it is necessary to carry out related duties continuously for a long period of time.

In order to improve the conventional method as described above, several bacterial detection mechanisms have emerged. However, these bacterial detection mechanisms and the like have a very complicated external structure and a very complicated internal structure, There is a disadvantage that it is cumbersome for the general public to use it effectively.

Prior art related to the conventional bacteria detection method and kit are as follows.

Japanese Patent Laid-Open No. 62-171671 entitled " Tampon for sample collection with culture container " (Jul. 28, 1987); Korean Patent No. 10-433784 entitled "Portable Microorganism Detector" (May 20, 2004); Korean Patent No. 10-453094 " Bacteria detection device " (2004. 10. 06); Korean Patent No. 10-1366965 "Food Poisoning Monitoring Kit" (Apr. 14, 2014); Korean Patent No. 10-1494978 " Food Poisoning Monitoring Kit " (Feb. 12, 2015); Korean Patent No. 10-1548513 " Method and medium for detecting the presence of a stigmatizing member of Staphylococcus aureus in a test sample " (Aug. 25, 2015)

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The present invention has been made to solve all the problems of the prior art described above, and it is an object of the present invention to provide a method and apparatus for identifying and distinguishing a person who holds human harmful bacteria from a large number of people to be inspected, The purpose of this paper is to provide a method for determining whether the

It is another object of the present invention to provide a method for determining whether or not food-borne bacteria are infected by using a safe and simple detection kit for bacteria.

In order to achieve the above-mentioned object, the present invention provides a method for producing a microorganism, which comprises a main body having a cylindrical shape and formed of glass, transparent or semitransparent plastic material and containing therein a selective medium for propagation and detection of bacteria; A specimen collecting unit for collecting harmful bacteria and for inoculating the collected specimens into the selective medium (M); Wherein the sample collection unit is held at a position spaced apart from the selection medium in a state in which one end of the sample collection unit is fixed and the sample collection unit does not collect the sample, and when the sample collection unit is collected, A selective regeneration unit which is immersed in the selective medium for inoculation; And a kit for detecting a harmful germ of a human body.

The main body portion is sealed in a rounded manner at its lower end, and has a selective medium accommodating portion containing the selective medium M therein, a central space portion having a center at a center thereof, It is preferable that an upper opening is formed.

The main body may be formed as a two-step separable type in which the selected-medium accommodating portion and the upper opening portion are detachable and detachable.

It is preferable that the sample collecting part has a long rod shape and includes a rod rod whose one end is detachably coupled to the selection control part and a sample collecting rod formed at the other end of the rod and capable of collecting the sample .

Preferably, the selection regulating unit includes a first stopper for coupling one end of the rod of the sample collecting unit and sealing the upper opening of the main body, and a second stopper provided above the first stopper in a state connected to the first stopper Do. It is preferable that a distance L 1 between the first stopper and the second stopper is longer than a distance L 2 between the selection medium M and the sample collecting rod.

The present invention also provides a method of analyzing a sample, comprising: separating the selection controller from a main body and collecting a sample from a subject using the sample collecting rod; The specimen collection rod taken in the specimen is inserted into the main body portion and force is applied so that the first stopper enters the inside of the main body portion and the second stopper comes in close contact with the end of the opening portion of the main body portion, So that the sample collecting rod is fully immersed in the selection medium of the main body part and inoculated; Culturing the selective medium of the body part in a 37 ° C incubator for 24 hours to 36 hours and discriminating specific bacteria by changing the color of the selective medium; .

When the kit according to the present invention is used, it is possible to confirm whether a specific bacterium is infected within 24 to 36 hours.

In addition, when the kit according to the invention is used, it is possible to quickly determine whether a particular bacterium is infected, so that the administrative agency can perform rapid follow-up on it, and a general person such as a foodservice worker, There is also an advantage of confirming the result.

In addition, when the kit according to the invention is used, since the selective medium in the kit is not a fluid having a fluidity as in the conventional art, the culture medium infected with the bacteria by the user's fruit flows out to the outside, There is also an advantage that there is no fear of leakage.

In addition, when the kit according to the present invention is used, since it can be partially recycled, saving of resources and prevention of environmental pollution can be achieved at the same time.

1 is a conceptual diagram of a kit for detecting bacteria according to the present invention,
FIG. 2 is a conceptual view showing a state of using a kit for detecting bacteria according to the present invention,
FIG. 3 is a conceptual diagram showing a decomposition state of a kit for detecting bacteria according to the present invention, which is a preferred embodiment in which the body part is divided into two parts,
FIG. 4A is a preferred embodiment showing the relationship between the sample collecting part and the selection controlling part of the present invention,
FIG. 4B is another preferred embodiment showing the relationship between the sample collecting part and the selection controlling part of the present invention,
5 is an enlarged cross-sectional view showing a coupling relationship between the main body part and the selection control part of the present invention.

Hereinafter, the present invention will be described in more detail and in detail. It is apparent that the present invention is not limited thereto and that the present invention is not limited thereto. In the specification of the present invention, the same reference numerals are used for the same parts, and parts that can be easily created by those having ordinary skill in the art are omitted in the description of the present invention. .

The present invention provides a kit (100) for detecting a bacterium which can identify a specific bacterium safely and quickly.

The bacterial detection kit 100 according to the present invention is formed in a cylindrical shape and includes a main body portion 110 accommodating a selective medium therein.

The main body 110 has a cylindrical shape in cross section and is formed of a glass material or a transparent or translucent plastic material. The main body 110 functions to accommodate a selective medium M that can reproduce and cultivate germs collected from the outside and then determine whether or not the germs are bacteria to be detected. When it is handled by the general public, it is preferable to be formed of a plastic material which is free from the risk of external damage, but it is not necessary to exclude the transparent glass.

The main body 110 is sealed at its lower end and has a selective medium accommodating portion 112 accommodating the selective medium M therein and a central space portion 114 and an upper opening 116 whose upper end is completely opened. It is preferable that the selective medium accommodating portion 112 is made of a transparent material in order to observe a change in color of the selective medium M present therein.

It is preferable that the selective medium (M) has non-flowability at room temperature. When the selective medium M is non-ferrous at room temperature, the main body 110 may not be raised in the process of using or managing the kit 100 of the present invention, or may be laid laterally or obliquely, There is no possibility that the selective medium M will flow out or leak from the selective medium accommodating portion 112. Further, in the selective medium M, when a specific bacterium is harvested and cultured, a coloring medium which changes the hue of the selected medium is used, or the shape of the selected medium is changed to judge whether or not the specific bacterium is infected Can be used.

Further, it is preferable that the human harmful bacteria suitable for the selective medium (M) are food poisoning bacteria. Examples of the food poisoning bacteria include Salmonella Thyphi , Salmonella spp ., Shigelladysentriae , Staphylococcus aureus , Escherichia Coli And the like.

In the present invention, it is preferable that the central space 114 surrounds the outer surface of the central space 114 with a label such as an indication of the product, usage, precautions, manufacturer, date of manufacture or the like. The upper opening 116 preferably has a plurality of protrusions 118 formed therein. The plurality of protrusions 118 keep the sample collecting rod 124 away from the selective medium M when the first stopper 132 is hooked to the first stopper 132, The sample collecting rod 124 is completely pinched in the selective medium M so that the sample can be inoculated into the selective medium. Therefore, when the user accidentally presses the selection control unit 130 by mistake, the sample collecting rod 124 is prevented from being pushed in at once, and the user can detect the carelessness.

In the present invention, it is preferable that the main body 110 divide the central space part 114 into two stages and use the separate medium accommodating part 112 and the upper opening part 116 separately from each other . In this case, the sample collecting rod 124 may be removed while the sample collecting rod 124 can be used to confirm whether or not a specific bacterium has been infected. Then, the sample collecting rod 124 may be used to perform another culture test or identification This is because there is an advantage in that the inoculum can be conveniently used. This means that a large number of bacteria detection tasks can be performed using the once obtained sample.

The kit 100 for detecting a bacterium according to the present invention includes a sample collecting unit 120 for collecting a sample and inoculating the collected sample into the selective medium M.

The specimen collecting unit 120 takes a predetermined specimen from a person to be inspected and transfers the specimen to a selective medium present inside the main body 110. The sample collecting unit 120 is completely detached from the selection medium M in a state in which the sample collecting unit 120 is detachably fixed to the selection controlling unit 130 and before the sample is collected and after the sample is collected . This is because the sample collecting unit 120 is coupled to the inside of the main body 110 and the sample collecting unit 120 is connected to the sample collecting unit 120 in accordance with the coupling position of the selection controlling unit 130 with respect to the main body 110. [ Which means that the contact patterns of the culture medium 120 with the selective medium M are different from each other, which is quite different from the conventional bacterial detection kits.

The sample collecting unit 120 includes a rod rod 122 having a long rod shape and a sample collecting rod 124 formed at the other end of the rod rod 122. One end of the rod of the rod 122 is coupled to the selection controller 130, while a sample collecting rod 124 is formed at the other end of the rod. The sample collecting rod 124 may be a conventionally used swab. It is preferable that the sample collecting rod 124 is coupled to the coupling hole 136 of the selective regulating part 136 in an interference fit manner. It is preferable that a plurality of the coupling grooves 136 are formed. When a plurality of coupling grooves 136 are formed in the selection and control unit 130, a plurality of sample collection rods 124 can be used for one selection control unit 130.

The bacterial detection kit 100 according to the present invention includes a selection controller 130 that fixes one end of the sample collection unit 120 and seals the opening 116 of the body 110.

The selection control unit 130 may fix one end of the sample collection unit 120 so that the user can conveniently collect an external sample while the sample collection unit 120 is connected to the main body 110 The main body 110 is sealed.

The selection controller 130 includes a first stopper 132 for coupling one end of the rod 122 of the sample collecting unit and sealing the upper opening 116 of the body 110, And a second stopper 134 located above the first stopper 134 in a state connected to the second stopper 132. The distance (L 1) spaced between the first stopper 132 and second stopper 132 is compared to the distance (L 2) spaced between the selective medium (M) and the sample collection rod 124 It is preferable that they are formed longer.

The sample regeneration unit 130 may be configured such that the sample collection rod 124 is inserted into the selective medium (not shown) while the one end of the sample collection unit 120 is fixed, M, while when the sample collecting rod 124 collects the sample, the sample collecting rod 124 enters into the selective medium M and cultivates the bacteria .

The principle is as follows.

1, the first stopper 132 of the selection control unit 130 is slightly inserted into the opening 116 when the sample collecting rod 124 has not yet sampled the specimen. At this time, the first stopper 132 is engaged with the projection 118 near the opening 116. In this state, the sample collecting rod 124 is located above the selective medium M and is not brought into contact with the selective medium M.

On the other hand, when the user inserts the sample collecting rod 124 into the main body 110 after infecting the bacteria at the site of bacterial infection using the sample collecting rod 124, So that the first stopper 132 is further lowered and the second stopper 134 is brought into close contact with the outer circumferential edge of the end of the opening 116 of the main body 110. In this state, the sample collecting rod 124 slips into the inside of the selective medium M, and the sample collected outside is brought into contact with the selective medium M and then cultured therein. This is possible because the separation distance L 1 of the stoppers is longer than the separation distance L 2 between the selection medium M and the sample collection rod 124.

Further, the present invention provides a method for quickly determining whether or not a bacterium is infected using the kit 100 for detecting a bacterium.

The present invention includes a step of separating the selection controller 130 from the main body 110 and collecting a specimen from a subject using the specimen collection rod 124. [

The sample collection step is the first step performed using the kit 100 for detecting bacteria according to the present invention. The user or the inspector separates the selection control unit 130 from the main body 110 by holding the bacterial detection kit 100 of the present invention by hand. When the selection controller 130 is detached from the main body 110, the sample collecting rod 124 can be freely used, and the sample is collected from the examinee. Depending on the bacterium to be detected, the part to be sampled may be different. In order to determine whether the subject is infected with food poisoning bacteria, the food poisoning bacteria are collected from excreta (eg, stool) derived from the site where the food poisoning bacteria are most present or from the site.

The present invention includes a step of inoculating and culturing a sample collection rod 124, which is a sample to be tested, into a selection medium M of the main body 110. [

In the step of inoculating and culturing the sample in the selective medium, the sample collecting rod 124 from which the sample is collected is inserted into the main body 110 and the force is applied to the first stopper 132, (110). Thereafter, more force is applied to the second stopper 134 until the second stopper 134 is in close contact with the opening 116 of the main body 110. When the second stopper 134 is brought into close contact with the end of the opening 116, the sample collecting rod 124 penetrates through the selective medium M of the main body 110, So that the specimen is inoculated into the selective medium (M).

When this condition is reached, the kit 100 of the present invention is delivered for culturing in an incubator. At this time, since the selective medium M is non-fluid at room temperature, its handling is free and safe.

The present invention is characterized in that the selective medium M of the main body 110 is cultured in a 37 ° C incubator for 24 hours to 36 hours and the change of the color of the selective medium M or the change of the shape of the selective medium M And a step of discriminating a specific germ. The culture of the selective medium (M) is preferably carried out in an incubator and can be carried out in a conventional manner.

Hereinafter, the present invention will be described as more specific examples.

One). Selection medium (M):

The selective medium (M) used in the present invention was a chromogenic medium produced by the applicant. The components of the selective medium (M) are shown in Table 1 below.

                       Contents of Medium (gm / 0.5 liter) Contents    Bacteriological Agar   6.0g    Peptone   5.0g    ChromogenicMix   14.0 g    Antibiotic   8.5 mg    Indicator   0.02 g    Samolnella activator   10ml    Distilled water   500ml

The components contained in the selective medium M include a nutrient component capable of growing microorganisms and provide a stable environment for the microbial growth of the microorganisms present in the specimen by the added nutrients, indicators, coloring agents, and the like Therefore, the microorganisms can be prevented from drying due to internal components and moisture of the medium even during transportation for confirmation inspection, and viability can be maintained. The selective medium was inserted into the selective medium accommodating portion 112 at the lower end of the main body 110.

2). Strain and sample

The strains were Salmonella Thyphi , Salmonella spp ., Shigelladysentriae , Staphylococcus aureus, Escherichia Coli , Klebsiellaspp ., Enterobacter spp . Citrobacterspp. Were diluted to a constant concentration and used.

3). Experimental Method

In order to confirm the color change according to the species in the selective medium, the results were observed using the strain. The concentration of the strain was diluted using a sterilized PBS buffer with a 0.5 McFarland standard solution as a control, and then inoculated with a sterilized swab 124. This was inoculated into the selected medium accommodating portion 112 inside the main body 110, In the selective medium (M). The main body 110 and the selective medium M were placed inside the incubator. The inside of the incubator was maintained at 37 ° C, and after 24-36 hrs of incubation, the color of the selective medium (M) was observed.

4). Experiment result

The experiment was carried out by inoculating 5 isolates of each of 8 strains into the selective medium (M) of different serial number 10 times. As a result, the chromagens corresponding to the strains inoculated into the chrome agar plates within 24 to 36 hours in all cases there was.

In particular, Salmonella spp . Group, Shigelladysentriae , and Staphylococcus aureus showed magenta (P) and other intestinal bacteria showed blue (B) color. (See Fig. 6)

Thus, when a specimen is obtained from those who are engaged in the public or entertainment establishments, and the specimen is inoculated into the selective medium according to Table 1, when magenta (P) , The person can be identified as a person infected with food poisoning bacteria.

For these people, government agencies such as public health centers judge them as carriers of food poisoning bacteria, and after that, they are allowed to go through prescribed administrative procedures. On the other hand, medical institutions such as hospitals conduct more precise inspections as foodborne pathogens, We will proceed to treatment of infection.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, The range is determined and limited.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the present invention.

100: kit for detecting bacteria (present invention)
110: main body portion, 112: selective medium accommodating portion,
114: central clearance, 116: upper opening,
118: protrusion, 120: specimen collection part,
122: rod rod, 124: sample collecting rod,
130: selection control unit, 132: first stopper,
134: second stopper, 136: engaging groove

Claims (8)

In a method for determining whether a food poisoning infection is caused by the excrement of persons engaged in public or entertainment,
A main body 110 formed in a cylindrical shape and made of glass, transparent or translucent plastic material and containing a selective medium M for propagation and detection of bacteria therein; A bar rod 122 having a long rod shape and one end of which is detachably coupled to the selection control part 130; A sample collecting part 120 formed at the other end of the rod 122 and including a sample collecting rod 124 capable of collecting a sample; One end of the specimen collection unit 120 is fixed and the specimen collection unit 120 is kept spaced apart from the selective medium M in a state in which the specimen collection unit 120 does not collect the specimen , And a selection control unit (130) for allowing the specimen collection unit (120) to worm the inside of the selective medium (M) and to be inoculated when the specimen collection unit (120) collects the specimen A kit 100 for detecting bacteria is used,
Obtaining the sample by a sample collecting rod 124 formed at the other end of a rod rod 122 having a long rod shape;
Placing the sample collecting rod (124) in a selective medium (M) inside the bacterial detection kit (100) to inoculate the sample;
Culturing the bacterial detection kit (100) in an incubator for 24 to 36 hours;
When the internal selection medium M of the bacterial detection kit 100 exhibits magenta color P when the bacterial detection kit 100 is visually observed, it is determined that the person is infected with food poisoning bacteria ; To
The method comprising the steps of:
The method according to claim 1,
The main body 110 is sealed at its lower end and has a selective medium accommodating portion 112 accommodating the selective medium M therein and a central space portion 114), and an upper opening (116) in which the upper end thereof is completely opened.
The method according to claim 1,
The selection controller 130 includes a first stopper 132 for coupling one end of the rod 122 of the sample collecting unit and sealing the upper opening 116 of the body 110, And a second stopper (134) located above the first stopper (132) while being connected to the second stopper (132).
The method of claim 3,
Wherein the main body 110 is formed as a two-stage detachable type that can be detached and attached by separating the accommodating portion 112 of the selective medium M and the upper opening 116 into two, Way.
5. The compound according to any one of claims 1 to 4,
The food poisoning bacteria to be inoculated into the selective medium (M) contained in the main body part (110) is any one selected from the group consisting of Salmonella spp., Shigelladysentriae, and Staphylococcus aureus . .
6. The method of claim 5,
Wherein said selective medium (M) is a color development medium.
The method according to claim 6,
The selective medium (M)
Contents of Medium (gm / 0.5 liter)
Bacteriological Agar 6.0g
Peptone 5.0 g
Colorant (ChromogenicMix) 14.0 g
Antibiotic 8.5mg
Indicator 0.02 g
Samolnella Activator 10ml
Distilled water 500ml
Wherein the method is used to determine whether or not a food poisoning infection is caused.
8. The method of claim 7,
Wherein the selective medium (M) is non-active at room temperature.

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