KR20170041971A - Feed additive for livestock comprising ginkgo leaves by-product as effective component and uses thereof - Google Patents
Feed additive for livestock comprising ginkgo leaves by-product as effective component and uses thereof Download PDFInfo
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- KR20170041971A KR20170041971A KR1020150141286A KR20150141286A KR20170041971A KR 20170041971 A KR20170041971 A KR 20170041971A KR 1020150141286 A KR1020150141286 A KR 1020150141286A KR 20150141286 A KR20150141286 A KR 20150141286A KR 20170041971 A KR20170041971 A KR 20170041971A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/32—Foods, ingredients or supplements having a functional effect on health having an effect on the health of the digestive tract
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2122—Gingko Biloba
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
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- Physiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The ginkgo leaf by-product, which is a feed additive of the present invention, is a by-product produced after extracting ginkgo leaf with methanol. Although it has not been used in the past, it contains a large amount of ingredients such as crude protein, crude fat, crude fiber, neutral detergent fiber (NDF) and acid detergent fiber The antioxidant activity and the ginkgo leaf by - product digestion reaction were also confirmed to contain active ingredients. Therefore, by using ginkgo leaf byproduct, which is a byproduct of methanol extract of ginkgo leaf, it is possible to reduce waste, and since the ingestion of Ginkgo biloba byproduct can also provide a sufficient utility value as a feed additive, it can be usefully used as a feed additive for livestock.
Description
The present invention relates to a feed additive for livestock containing a ginkgo leaf by-product as an active ingredient, and a use thereof, and more particularly to a feed additive for livestock containing a ginkgo leaf by-product as an active ingredient and a feed for livestock ≪ / RTI >
Recently, as the level of public awareness of health has increased, there is a strong interest in environmentally friendly livestock products that are safe and have high quality. In the past, antibiotics and various growth promoting agents have been developed to improve the productivity of livestock products. However, due to the problem of tolerance remaining in livestock products, there is a need to suppress the harmful bacteria of the gastrointestinal tract and to increase the homeostasis such as immunity enhancement Feed additives have been developed. A variety of physiologically active substances, such as organic acids, flavonoids and β-carotene, isolated from natural products among feed additives, are effective in preventing diseases by enhancing the immune system and maintaining homeostasis in domestic animals. In particular, there are various plant physiologically active substances in natural plants, among which the flavonoids are the most abundant.
On the other hand, Ginkgo biloba L. is a main active ingredient and has been widely used as an active ingredient in foods such as Flavonol glycoside, proanthocyanidin, organic acid, bilobalide, ginkgolide) A, B, C, and J have been reported. In particular, ginkgo leaf extract is used for the treatment of free radical related diseases such as anoxia and diseases such as brain and cardiac ischemia, arteriosclerosis and cancer. The activity of the Ginkgo biloba extract having such a potency is mainly determined by the flavonoid content having antioxidant ability.
Korean Patent No. 0888939 discloses a method for producing ginkgo leaf feed and ginkgo leaf feed. However, the feed additives for livestock containing the ginkgo leaf by-product of the present invention as an active ingredient and its use have not been mentioned yet.
SUMMARY OF THE INVENTION The present invention has been made in view of the above-described needs, and an object of the present invention is to provide a method of extracting active ingredients from ginkgo leaves using ginkgo biloba, crude fat, crude fiber, neutral detergent fiber (ADF) acid detergent fiber), antioxidant activity, and active ingredient in ginkgo leaf byproduct digestive juice reaction. Accordingly, the present invention has been accomplished by confirming that the ginkgo leaf by-product, which has been regarded as wastes through the present invention, can provide sufficient utility value as a feed additive in case of feed to livestock without extra processing.
To achieve the above object, the present invention provides a feed additive for livestock containing as an active ingredient ginkgo biloba by-product.
In addition, the present invention provides a feed composition for livestock incorporating the feed additives for livestock.
The ginkgo leaf by-product, which is a feed additive of the present invention, is a by-product produced after extracting ginkgo leaf with methanol. Although it has not been used in the past, it contains a large amount of ingredients such as crude protein, crude fat, crude fiber, neutral detergent fiber (NDF) and acid detergent fiber The antioxidant activity and the ginkgo leaf by - product digestion reaction were also confirmed to contain active ingredients. Therefore, it is possible to reduce the waste by using the ginkgo leaf byproduct produced after extracting the ginkgo leaf with methanol, and the ingestion of the ginkgo leaf byproduct can also give a sufficient utility value as a feed additive.
FIG. 1A shows the result of analysis of the active ingredient content before extracting ginkgo leaf with methanol, FIG. 1B shows the result of extracting gingko leaf with methanol Generated It is the result of analysis of the active ingredient content of byproduct of ginkgo leaf.
Fig. 2 shows the result of measuring the antioxidative activity of the by-product of the ginkgo leaf of the present invention. CFW is freeze drying of ginkgo leaf, CNW is natural drying of ginkgo leaf, CHW is ginkgo dry, BFW is freeze drying of ginkgo leaf, BNW is natural drying of ginkgo leaf byproduct, and BHW is ginkgo leaf byproduct drying.
FIG. 3 shows the results of HPLC analysis of digestive juice reactant of the ginkgo leaf and ginkgo leaf byproducts of the present invention.
FIG. 4 shows the results of weighing of pigs fed with general diets containing general diets and by-products of Ginkgo biloba.
In order to achieve the object of the present invention, the present invention provides a feed additive for livestock containing as an active ingredient ginkgo biloba.
The ginkgo leaf by-product of the present invention comprises 1) adding ginkgo leaf to methanol, extracting the mixture at 60 to 80 ° C for 3 to 5 hours, and then centrifuging; And
2) removing the supernatant after centrifugation in step 1), and separating the remaining ginkgo leaf byproduct,
Preferably,
1) adding 5 to 20 parts by weight of ginkgo biloba to 100 parts by weight of 100% (v / v) methanol, extracting the mixture at 60 to 80 ° C for 3 to 5 hours, and centrifuging; And
2) removing the supernatant after centrifugation in step 1), and separating the remaining ginkgo leaf byproduct,
More preferably,
1) adding 10 parts by weight of ginkgo leaf to 100 parts by weight of 100% (v / v) methanol, extracting at 70 ° C for 4 hours and centrifuging; And
2) After centrifuging in step 1), the supernatant is removed and the remaining ginkgo leaf by-products are separated, but the present invention is not limited thereto.
In one embodiment of the invention, the animal may be a pig, but is not limited thereto.
In one embodiment of the invention, the ginkgo leaf by-product may have antioxidant activity, but is not limited to this.
In one embodiment of the invention, the feed additive may additionally contain a carrier which is acceptable to mammals. In the present invention, the feed additive may be added as it is or a known carrier, stabilizer and the like may be added. Various nutrients such as vitamins, amino acids and minerals, antioxidants, antibiotics, antibacterial agents and other additives may be added And the shape thereof may be a suitable state such as powder, granule, pellet, suspension and the like.
In addition, the present invention provides a feed composition for livestock incorporating the animal feed additive.
In one embodiment of the present invention, the feed additives for livestock may be formulated in an amount of 1.5 to 4.5% by weight, preferably 3% by weight, based on feed composition for livestock, but are not limited thereto.
The present invention also provides a method for raising livestock by feeding the livestock feed composition.
1) adding 5-20 parts by weight of ginkgo biloba to 100 parts by weight of 100% (v / v) methanol, extracting the mixture at 60 to 80 ° C for 3 to 5 hours, and then centrifuging; And 2) a step of removing the supernatant after centrifugation in step 1) and separating the remaining ginkgo leaf byproducts, thereby providing a method for preparing a feed additive for livestock containing an extract of ginkgo biloba as an active ingredient.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.
Example 1. Preparation of the Ginkgo leaf by-product of the present invention
10 g of ginkgo leaf was added to 100 ml of 100% (v / v) methanol, and the mixture was centrifuged at 70 ° C for 4 hours. After centrifugation, the supernatant was removed and the remaining ginkgo leaf by-products were separated and used in the present invention.
Example 2. Analysis of constituents of the ginkgo leaf by-products of the present invention
In order to use the by-product of Ginkgo biloba as a feed additive, we requested composition analysis of ginkgo leaf by-product in Feed Research Institute of Korea Dairy Feed Association. As a result, as shown in Fig. 1, the gingko leaf was extracted with methanol Generated When the ingredient content of ginkgo leaf byproduct (FIG. 1B) and the ingredient content of Ginkgo leaf before methanol extraction (FIG. 1A) were compared, the contents of crude protein, crude fiber and calcium were increased. It was analyzed as worthy.
Example 3. Functional investigation of the ginkgo leaf by-product of the present invention
1) Antioxidant activity measurement
Antioxidant activity was measured by DPPH and ABTS assay according to the drying conditions (freezing, hot air and nature) after methanol extraction in order to confirm the effect of enhancing the immunity and physiological activity of the ginkgo leaf of the present invention.
DPPH analysis is the most widely used method in in vitro antioxidant experiments. DPPH (1,1-diphenyl-2-picrylhydrazyl) is purple when it is in a radical state and changes from a stable state without a radical to yellow The radical scavenging ability was confirmed. A sample of the ginkgo leaf and a reference material (Vitamin C) were prepared by diluting each concentration, and a DPPH solution of 50 μM was prepared. The OD of the DPPH solution was diluted to 0.7 at a wavelength of 517 nm. 0.05 ml of sample and 1.45 ml of DPPH solution were mixed and reacted in the dark for 30 minutes, and the absorbance was measured using a spectrophotometer.
The ABTS analysis showed that the free radical scavenging ability of the ginkgo leaf byproducts for stable oxidized free radicals (ABTS) (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) The antioxidant capacity of each sample was tested and compared with Vitamin C as a positive control. A radical solution prepared by adding 1.0 mM of AAPH (2,2'-azobis- (2-amidinopropane) hydrochloride) and 2.5 mM of ABTS to 100 ml of PBS was prepared and incubated at 30 The mixture was heated while stirring for a minute, and allowed to cool to room temperature. The radical solution was filtered using 0.45 占 퐉 PVDF (polyvinylidene fluoride), diluted to show an absorbance of 0.65 at a wavelength of 734 nm, 20 占 퐇 of sample and 980 占 퐇 of radical solution were mixed and reacted at 37 占 폚 for 10 minutes The absorbance at 734 nm was measured and the antioxidant power was calculated using the following equation.
Antioxidant capacity (%) = 100-100 * (OD sample - OD blank ) / (OD control - OD blank )
(OD blank : Absorption of ethanol, OD control : Absorbance when the concentration of the sample is 0)
As shown in FIG. 2, DPPH analysis showed that the antioxidative activities of the samples obtained by lyophilization (BFW and CFW) of Sample B (Ginkgo biloba byproduct) and C (Ginkgo leaf) BHW and CHW) samples showed antioxidant activity. Thus, it was confirmed that the ginkgo leaf by-product of the present invention had antioxidative activity regardless of the drying condition.
2) Analysis of active ingredient of digestive juice
Ginkgo biloba and ginkgo leaf byproducts were finely ground and each 2 g was placed in a 50 ml tube and 15 ml of saline and 3 ml of pepsin (0.04 g pepsin in 0.1 M HCl) were added and the pH was adjusted to 2, And reacted for 1 hour in a constant temperature water bath at 37 ° C. After the reaction, the pH was adjusted to 5.3 with 0.9 M sodium bicarbonate, and then 600 μl of bile salt (0.04 g bile in 1 ml of saline) and 300 μl of pancreatin And the pH was adjusted to 7.4 with 1M NaOH solution. The reaction was carried out at 37 ° C for 2 hours. After the reaction was completed, the mixture was boiled at 100 ° C for 20 minutes to inactivate the enzyme, and the reaction mixture was centrifuged at 8000 rpm for 20 minutes to obtain a supernatant, which was filtered through a 0.45 μm filter and used for HPLC analysis.
As a result, as shown in FIG. 3, the digestive juices reacted with ginkgo leaf and ginkgo leaf byproducts in a similar pattern, but relatively fewer ginkgo leaf by-products than ginkgo leaves. The reason for this is that some of the useful components were extracted from Ginkgo biloba leaf using methanol. In addition, the two peaks having the highest peaks of the compound A and the compound B were selected and the overall peak area was compared. Ginkgoride A was quantified using a standard material. As a result, as shown in Table 1, only about 30% of the ginkgo leaf byproducts were present in the ginkgo leaf by-product. However, in view of the fact that the ginkgo leaf by-product, which has been left unused in the past, can be used as a feed additive, the content of the useful ingredient of the ginkgo leaf by- product does not reach the ginkgo leaf but a considerable amount remains. It is believed that it is possible to make value.
(peak area)
± 286504
± 161407
(peak area)
± 147208
± 48586
Example 4. Feed additives of the ginkgo leaf by-product of the present invention On the payroll Investigation of preference according to
The control group (100% of the general feed powder) and the test group (97% of the general feed powder + 3% of the ginkgo leaf byproduct powder) were fed to the piglets for 2 weeks. As a result, as shown in Fig. 4, when the results of the control group and the test group were compared, the weight of the piglets tended to increase in the test group, compared with the control group in which the general feed powder was used as 100% The weight of piglets tended to increase much more than the control group. In other words, the consumption of Ginkgo biloba by - product could be a useful additive for feed additives.
Claims (6)
1) adding methanol to the ginkgo leaf, extracting it at 60 to 80 ° C for 3 to 5 hours, and then centrifuging; And
2) removing the supernatant after centrifugation in step 1), and separating the remaining ginkgo leaf byproducts.
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| KR20210131629A (en) | 2020-04-24 | 2021-11-03 | 서울대학교산학협력단 | Feed composition for livestock using Methionine hydroxy analogue and preparing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20210131629A (en) | 2020-04-24 | 2021-11-03 | 서울대학교산학협력단 | Feed composition for livestock using Methionine hydroxy analogue and preparing method thereof |
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