KR20170028489A

KR20170028489A - Tilting microscope

Info

Publication number: KR20170028489A
Application number: KR1020150124859A
Authority: KR
Inventors: 고국원; 이상준
Original assignee: 선문대학교 산학협력단
Priority date: 2015-09-03
Filing date: 2015-09-03
Publication date: 2017-03-14
Also published as: KR101732923B1

Abstract

A tilting microscope according to the present invention includes an enlargement unit in which an enlarged image of a sample is generated; An optical unit for generating light input to the enlargement unit through the sample; And a moving unit that rotates the magnifying unit about the sample while the sample is fixed.

Description

{TILTING MICROSCOPE}

The present invention relates to a microscope for magnifying and observing a sample as an object to be observed.

The microscope is a mechanism for magnifying and observing microscopic objects or microorganisms that can not be observed with the human eye.

The microscope can be used to sample the sample. A sample may refer to a substance or organism provided for scientific testing, testing, or chemical analysis with a specific purpose.

Korean Patent Laid-Open Publication No. 2007-0114718 discloses a microscope capable of supplying or recovering liquid at the time of observation by local immersion.

Korean Patent Publication No. 2007-0114718

The present invention is to provide a tilting microscope capable of observing a sample in a plurality of directions.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise forms disclosed. Other objects, which will be apparent to those skilled in the art, It will be possible.

The microscope of the present invention includes an enlargement unit in which a magnified image of a sample is generated; An optical unit for generating light input to the enlargement unit through the sample; And a moving unit that rotates the magnifying unit about the sample while the sample is fixed.

The microscope of the present invention may be provided with an enlarging unit that rotates around the sample. Therefore, the sample can be observed from various angles while the sample is fixed.

1 is a schematic view showing a microscope of the present invention.
2 is a perspective view of a microscope of the present invention.
3 is a schematic diagram showing the operation of the microscope of the present invention.
4 is a schematic view showing a magnifying unit;

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The sizes and shapes of the components shown in the drawings may be exaggerated for clarity and convenience. In addition, terms defined in consideration of the configuration and operation of the present invention may be changed according to the intention or custom of the user, the operator. Definitions of these terms should be based on the content of this specification.

1 is a schematic view showing a microscope of the present invention.

1 may include a magnifying unit 100, a light unit 200, and a mobile unit 300. [

An enlarged image of the sample 10 can be generated by the enlargement unit 100. [ The enlargement unit 100 may be provided with a lens unit 110 having a convex lens or the like so that an enlarged image of the sample 10 is generated.

The sample 10 to be observed can be observed while being covered with the transparent slide 30. Due to the geometric characteristics of the slide 30, the enlargement unit 100 for enlarging the image of the sample 10 can be disposed on one side of the slide 30. The light source unit may be disposed on the other side of the slide 30.

The light source unit can generate light input to the enlargement unit 100 through the sample 10.

Since the enlargement unit 100 is disposed on one side of the slide 30, the image obtained from the enlargement unit 100 is limited to the image viewed from the sample 10 at a position facing the slide 30 . Therefore, the user can observe only the front surface of the sample 10 through the microscope.

If it is necessary to observe the side surface or the back surface of the sample 10, the user should observe the slide 30 by tilting it. Therefore, when it is necessary to observe the sample 10 in a plurality of directions, it may be inconvenient for the user to change the arrangement state of the slide 30. [ In addition, during the process of tilting or reversing the slide 30, the sample 10 mounted on the slide 30 may flow down or the activity state of the cells may be changed. In this state, it is clear that the reliability of the observation of the sample 10 is lowered.

As a result, even if it is necessary to observe the sample 10 in various directions, it is preferable to keep the slide 30 on which the sample 10 is mounted fixed.

The moving unit 300 can be used to observe the sample 10 in various directions with the sample 10 fixed.

The moving unit 300 can rotate the magnifying unit 100 around the sample 10 in a fixed state. At this time, the moving unit 300 moves the enlargement unit 100 while keeping the optical axis O1 of the lens unit 110 toward the sample 10 and maintaining the distance D between the sample 10 and the enlargement unit 100 . D is kept constant, the focal point of the magnifying unit 100 can be kept constant without changing even if the position of the magnifying unit 100 is changed.

The mobile unit 300 may be variously formed.

For example, as shown in FIG. 1, the moving unit 300 may include a spherical guide that rotates about the sample 10 so that the sample 10 can be observed in various directions from front to back, left and right. The optical axis O1 of the enlargement unit 100 is always directed to the sample 10 regardless of the rotation of the guide when the enlargement unit 100 is disposed such that the optical axis O1 faces the sample 10 at one point of the moving unit 300 . Further, since the radius of the sphere is constant, D can also be kept constant regardless of the rotation of the guide. In addition, since the spherical guide can be rotated in various directions around the sample 10, the enlarging unit 100 can be moved in a direction desired by the user.

As a result, according to the mobile unit 300 of the present invention, the user can observe not only the front view of the sample 10 but also all directions including the rear view and the side view.

2 is a perspective view of a microscope of the present invention.

The guide in which the enlargement unit 100 is installed in the mobile unit 300 does not necessarily have to have a spherical shape. It is only necessary to rotate the enlargement unit 100 along the surface of the virtual sphere about the sample 10. Therefore, the guide can be formed variously in consideration of convenience of manufacture or convenience of control.

For example, the moving unit 300 may be provided with a first guide 310 and a second guide 320 in an annular shape.

The first guide 310 may be formed in a circular shape with the sample 10 as a center. The enlargement unit 100 may be installed on the first guide 310 so as to be movable along the circumference. According to the first guide 310, the magnifying unit 100 may have only a first rotational degree of freedom centering on a virtual line orthogonal to the first guide 310, instead of the rotational degree of freedom around the point.

A second guide 320 may be used to extend the first rotational degree of freedom to a rotational degree of freedom about the point.

The second guide 320 can support the first guide 310 in a rotatable manner. When the diameter of the first guide 310 connects the first position of the first guide 310 and the second position, the fulcrum of the first guide 310 by the second guide 320 is located at the first and second positions, Position.

According to the second guide 320, the first guide 310 may have a second rotational degree of freedom about an imaginary line extending along the upper diameter. Accordingly, the first guide 310 and the second guide 320 can have both the first rotation degree and the second rotation degree of freedom. This state can be similar to a state in which the enlargement unit 100 has a rotation degree of freedom around a point.

On the other hand, according to the enlargement unit 100 which tilts at various angles, light emitted from the light source unit may hardly be input to the enlargement unit 100. The enlargement unit 100 can be moved to the rear side of the slide 30 by the moving unit 300 in a state where the light source unit is disposed on the rear side of the slide 30. [ When the enlargement unit 100 and the light source unit are disposed in the same direction, light irradiated from the light source unit must be reflected by the sample 10 and then input to the light source unit. However, depending on the configuration of the light source unit, reflection of light may be difficult. Even if light reflection is possible, only a part of the light irradiated from the light source unit is inputted to the enlargement unit 100, so that the brightness of the obtained image will become very dark.

The moving unit 300 can move the optical unit 200 as well as the enlargement unit 100 so that a clear image can be obtained regardless of the movement of the enlargement unit 100. [

1, the moving unit 300 is configured to move the sample 10 so that the sample 10 and the optical unit 200 are disposed on the optical axis O1 of the lens unit 110 irrespective of the movement of the magnifying unit 100. [ The magnifying unit 100 and the optical unit 200 can be rotated together.

The optical unit 200 may be disposed at a position facing the sample 10 in a guide provided in the mobile unit 300 so as to be rotated together with the magnification unit 100. [

On the other hand, the optical unit 200 can be moved along a direction perpendicular to the optical axis O1.

When the optical unit 200 is arranged at an angle of 90 degrees with respect to the slide 30 as shown in Fig. 1 and the optical unit 200 is arranged at an angle of -90 with respect to the slide 30, It is preferable that the optical axis O2 of the enlargement unit 100 coincides with the optical axis O1 of the enlargement unit 100. [

According to the optical unit 200 that is rotated with the enlargement unit 100 at a position opposite to the enlargement unit 100 on the basis of the sample 10, O1 and O2 can coincide with each other regardless of how the enlargement unit 100 is rotated . However, it is not always preferable that O1 and O2 always match.

3 is a schematic diagram showing the operation of the microscope of the present invention.

For example, it is assumed that the optical unit 200 irradiates a short wavelength light such as a laser toward the magnification unit 100. The short wavelength light irradiated from the optical unit 200 due to the coincidence of O1 and O2 can be expected to be inputted to the enlargement unit 100 accurately through the sample 10. [ However, the short wavelength light may not be input to the enlargement unit 100 according to the rotation angle.

The sample 10 is mounted on a slide 30 having a predetermined thickness, in which light passing through the slide 30 is refracted. The axis of the short wavelength light passing through the slide 30 is shifted from the axis of the short wavelength light input to the slide 30 due to the refraction at this time. If O1 and O2 coincide with each other, the short wavelength light can not be input to the enlargement unit 100 due to the shifted distance F1.

The optical unit 200 is shifted in the direction a perpendicular to the optical axis of the mobile unit 300 during the rotation so that light is input to the zoom unit 100 regardless of the refraction of light generated in the slide 30 .

Specifically, the optical axis O2 of the optical unit 200 can be divided into a first optical axis extending from the optical unit 200 to the slide 30 and a second optical axis 2 extending from the slide 30 to the movable unit 300 .

At this time, the first optical axis? And the second optical axis? Can be displaced by F1 by the light refraction due to the slide 30. For reference, the light input to the slide 30 along the first direction travels in the second direction within the slide 30, but may proceed in the first direction after passing through the slide 30.

Since the light input to the enlargement unit 100 travels along the second optical axis 2, it is preferable that the optical axis O1 of the enlargement unit 100 coincides with the second optical axis 2 without considering the first optical axis. The movable unit 300 is moved in the direction of a direction from the second optical axis 2 to the first optical axis by an interval F1 between the first optical axis and the second optical axis so that the second optical axis 2 coincides with the optical axis O1 of the moving unit 300 The optical unit 200 can be shifted.

According to the magnifying unit 100, the optical unit 200 and the moving unit 300 described above, it is possible to observe the sample 10 at various angles while moving the magnifying unit 100 while fixing the sample 10 can do. According to the optical unit 200 that is rotated together with the magnification unit 100 and is shifted along the direction perpendicular to the optical axis, the maximum luminous flux can be provided to the magnification unit 100 regardless of the viewing angle. Therefore, a clear image can be obtained irrespective of the viewing angle.

The enlargement unit 100 may be formed in various ways.

4 is a schematic view showing the enlargement unit 100. Fig.

For example, the zoom unit 100 may include a lens unit 110 and a photographing unit 130.

The lens unit 110 can magnify the image of the sample 10. [

The lens unit 110 may be provided with an objective lens 111 and an auxiliary lens 113. The objective lens 111 is disposed between the photographing unit 130 and the sample 10 and can focus the light input from the sample 10 to provide an enlarged image. The auxiliary lens 113 is disposed between the photographing unit 130 and the objective lens 111 and can convert the light output from the objective lens 111 into a parallel beam.

The photographing unit 130 can photograph an enlarged image output from the lens unit 110. [ The photographing unit 130 may store the image as image data. The image photographed by the photographing unit 130 may be input to the analyzer 90. The analyzer 90 can process the image or analyze the characteristics of the sample 10 using the image.

The photographing unit 130 may include an optical camera, a photoelectric detector, and the like. For example, the photographing unit 130 may be provided with a polarizing plate 131, a condenser lens 133, a slit 135, a collimator lens 137, and a photoelectric detector 139.

The polarizing plate 131 can polarize the light that has passed through the objective lens 111.

The condensing lens 133 can condense the beam that has passed through the polarizing plate 131.

The slit portion 135 can filter the condensed beam at the condenser lens 133. [

The collimator lens 137 can convert the beam passing through the slit of the slit into a parallel beam.

The photoelectric detector 139 photoelectrically converts the parallel beam converted by the collimator lens 137 and outputs the converted electrical signal to the analyzer 90. [

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. Accordingly, the true scope of the present invention should be determined by the following claims.

10 ... sample 30 ... slide
90 ... Analyzer 100 ... Expansion unit
110 ... lens unit 111 ... objective lens
113 ... auxiliary lens 130 ... photographing section
131 ... polarizer 133 ... condenser lens
135 ... slit part 137 ... collimated lens
139 ... photoelectric detector 200 ... optical unit

Claims

An enlargement unit in which an enlarged image of the sample is generated;
An optical unit for generating light input to the enlargement unit through the sample; And
A moving unit for rotating the magnifying unit about the sample in a state where the sample is fixed;
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The method according to claim 1,
Wherein the enlargement unit is provided with a lens unit in which an enlarged image of the sample is generated,
Wherein the moving unit moves the zoom unit while the optical axis of the lens unit faces the sample and maintains the same distance between the sample and the zoom unit.

The method according to claim 1,
Wherein the enlargement unit is provided with a lens unit in which an enlarged image of the sample is generated,
Wherein the moving unit rotates the zoom unit and the optical unit around the sample so that the sample and the optical unit are disposed on the optical axis of the lens unit.

The method according to claim 1,
The moving unit rotates the optical unit and the magnifying unit around the sample,
The optical unit irradiates the short wavelength light toward the enlargement unit,
The sample is mounted on a slide having a predetermined thickness,
Wherein the optical unit is shifted in a direction perpendicular to an optical axis of the movable unit during the rotation.

The method according to claim 1,
The moving unit rotates the optical unit and the magnifying unit around the sample,
The sample is mounted on a slide having a predetermined thickness,
When the optical axis of the optical unit is divided into a first optical axis extending from the optical unit to the slide and a second optical axis extending from the slide to the moving unit, the first optical axis and the second optical axis And,
Wherein the moving unit includes a microscope for shifting the optical unit from the second optical axis toward the first optical axis by an interval between the first optical axis and the second optical axis so that the second optical axis coincides with the optical axis of the moving unit .