KR20160109093A - Method of preparing combined extract powder of fermented scutellariae radix and gastrodiae rhizoma from compounding solution of micro-organism and natural enzyne - Google Patents

Abstract

The present invention relates to a composite powder of fermented Scutellaria root and gastrodia, which is produced by the following steps: fermenting Scutellaria root using microorganism and natural enzyme; producing fermented Scutellaria root after undergoing aging and drying processes; mixing the fermented Scutellaria root with gastrodia, dried orange peel, pueraria, and ginseng; and extracting the same with a solvent, concentrating the same, and spray-drying the same. According to the present invention, the content of active components increases in the fermented Scutellaria root and the composite powder extract of the fermented Scutellaria root and gastrodia, and antioxidant activity against active oxygen is enhanced as well. Thus, the composite powder extract of the present invention exhibits excellent efficacy of preventing and ameliorating hyperlipidemia, arteriosclerosis, cerebrovascular disease, and cranial nerve disease and also improving memory as well.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for preparing a fermented mixture of chrysanthemum fermented with a microorganism and a natural enzyme,

The present invention relates to a process for producing fermented gold by fermentation of gold using microorganisms and natural enzymes, followed by aging and drying processes, and then fermented gold is mixed with chymase, dermis, gruel and ginseng and extracted with a solvent, The present invention also relates to a fermented golden chromatin composite powder.

Golden (Scutellaria radix) is a peeled root of Scutellaria baicalensis. It is native to Korea, China, Japan, and has greenish purple and bitter taste. The active ingredient of gold is Flavonoid which is the most abundant of Baicaline and Baicalein, Wogonine, Oroxylin A, Flabanone, Skullcapflavone, and the like.

The pharmacological action of gold is known to have mainly antipyretic action, antiinflammatory action, dandruff action, atherosclerosis prevention action, hypotensive action, sedation, hepatocyte protective action, nerve cell protective action and antitumor action. An allergic action, a respiratory disease improvement action, an autoimmune disease prevention action, and a diabetic improvement action. In the oriental medicine, it is prescribing as the anti-inflammatory drug mainly inflammation, hyperemia and fever accompanied diseases such as hypertrophic jaundice, headache, inflammatory conjunctivitis, respiratory infection, gastritis, enteritis,

Among the active ingredients of gold, studies on baicalin, baicalein and wogonin have been actively carried out. Among them, baicalin promotes secretion of bile, inhibits the physiological function of camokine and exhibits anti-inflammatory activity , Baicalein has a much higher antioxidant activity than baicalin, which is effective for circulatory diseases such as blood pressure lowering action, atherosclerosis preventing action, neuronal cell protection action and angiogenesis inhibitory action as well as antipyretic action. Have been reported to exhibit anti-inflammatory action as well as hepatocyte protective action and neuronal protective action.

GASTRODIAE RIZOMA is an orchidaceous plant stem that has a glossy, keratinized shape and has both sweet and savory flavor. The active ingredient of the horse root is a phenol derivative such as gastrodin 4-hydroxybenzylalcohol, 4-hydroxybenzaldehyde, vanillin, vanillyl alcohol and gas Gastrol and the like. It also contains phytosterol derivatives such as β-sitosterol, daucosterol and campesterol.

The pharmacological actions of Chunma are known to induce biliary secretion, hepatic interactions, and smooth muscle relaxation, as well as sedation, analgesic action, analgesic action, and brain cell protective action. In the oriental medicine, it is mainly prescribed as calming, oriental medicine, such as headache, dizziness, hysteria, fullness, hemiplegia, cramps, facial paralysis, pain and rheumatism.

Studies on gastrodine, 4-hydroxybenzaldehyde, vanillin, and the like have been actively carried out as active ingredients of Ganoderma lucidum, among which gastrodine restores and improves memory disorder and suppresses the neurotransmitter GABA 4-hydroxybenzaldehyde and vanillin have been reported to inhibit apoptosis in neuronal cells and enhance neurotransmitter action associated with GABA.

 The dermis (Aurantii Nobilis Pericarpium) is a mature rind of citrus unshiu (Citrus unshiu) with a peculiar odor and a slightly irritating flavor. The active ingredient of the dermis is Flavonoid which contains the most Hesperidin and also contains Rutin, Naringin, Neohesperidin and so on. It also contains limonoids, alkaloids and essential oils.

The pharmacological action of the dermis is mainly known as capillary lowering action, blood lipid lowering action, antiallergic action, antiinflammatory action, dryness action, vaginal action and antitumor action. In one room, it is prescribe mainly as dyspepsia, cough accompanied with sputum, and the like.

Studies on hesperidin as an active ingredient of dermis have been actively conducted, and research results such as capillary-lowering action, blood lipid lowering action, and anti-allergic action have been reported.

Puerariae Radix is a bamboo root of Pueraria lobata that is odorless and tastes sweet. The active ingredient of Pueraria is isoflavonoid which contains Puerarin, Daidzein, Daidzin and Genistein and is a triterpenoid. Soyasaponin, Soyasapogenol A and the like. The pharmacological actions of Puerariae are known to be antipyretic, antipyretic, circulatory, hypoglycemic, hepatoprotective, alcoholic, antithrombotic, and allergic. In one room, cold, fever, pain and muscular pain are mainly prescribed as cold flushing, fever, and asthma.

Studies on isoflavonoids have been actively conducted as an active ingredient of P. grisea. Among them, fuerelin, daidzin, and daidzein have been known to have antipyretic action, antipyretic action, hypoglycemic action, platelet aggregation inhibitory action, Have been reported.

Ginseng (Radix Alba) belongs to the family Araliaceae and has an unusual smell. The taste is slightly sweet at first and then bitter afterwards.

The active ingredient of ginseng is mainly Dammarane saponin, which contains protopanaxadiol derivatives Ginsenoside Ra1, Ra2, Rb1, Rb2, Rb3, Rc, Rd, (Ginsenoside) Re, Rf, Rg1, Rg2, Rh1 and the like, which are Protopanaxatriol derivatives, and Oleanane saponin and Ginsenoside Ro.

The pharmacological action of ginseng inhibits blood triglyceride production, prevents thrombogenesis, promotes blood circulation by inhibiting platelet coagulation, stimulates the cerebral cortex to promote peripheral blood flow, and improves blood circulation in the gastric wall have. It also enhances the protein synthesis ability of bone marrow cells and promotes erythropoiesis, accelerates the excitement conduction of the nerves, enhances the condition reflection ability to alleviate fatigue, enhances the secretion of digestive juices, and strengthens appetite And stimulates the peristalsis of the stomach to promote digestion and absorption. In addition, it has a lipid-decomposing action, and it is known to exhibit prevention and improvement effects of fatty liver due to alcoholic liquor, and to enhance gastrointestinal motility along with tonic action.

Studies on various ginsenosides as an active ingredient of ginseng have been actively carried out. Immunostimulation, action on the central nervous system, action on cardiovascular, hypoglycemic action, action on lipid metabolism, anti-cancer action, hepatocyte protective action , Learning ability improvement action, fatigue recovery and stress reduction action, and hair growth promoting action have been reported.

There is an attempt to extract active ingredients of gold or horse meat and use them as medicines or functional beverages. However, there is a problem in that the active ingredient is not greatly increased in the process of producing the extract, but rather is decreased or the active ingredient can not be uniformly extracted. In addition, there is a need for a composite powder containing all of pharmacological components such as gold, chymase, dermis, pupa, and ginseng for increasing and preventing cerebrovascular disease and brain nerve disease, and increasing their active ingredients.

The present invention provides a composite powder which increases antioxidative activity of golden herbal medicine and enhances neurotransmitter action of chimpanzee.

The present invention is to provide a composite powder containing all of pharmacological components such as gold, chymus, dermis, pupa, ginseng and the like and also having an increased active ingredient.

The present invention provides a composite powder that is effective for preventing and improving hyperlipidemia, arteriosclerosis, cerebrovascular disease, memory improvement, and brain cirrhosis.

The present invention relates to a method for fermenting a fermented microorganism, comprising the steps of: putting a microorganism-enzyme complex fermentation broth obtained by mixing a concentrated microorganism powder and a fruit enzyme concentrate into a scutellaria radix;

Fermenting the gold; And

And aging the fermented trihydrate for a predetermined period of time and drying the microorganism, wherein the microorganism is selected from the group consisting of Lactobacillus acidophilus MG501, Streptococcus thermophilus, and Saccharomyces cerevisiae cerevisiae. < / RTI >

The present invention relates to a method for preparing a fermented golden, chestnut, dermis, pupa, and ginseng complex extract prepared as described above;

A step of preparing a complex concentrate which is obtained by concentrating the complex extract in a concentrator and heating under reduced pressure; And

Adding purified water to the complex concentrate, and spray drying the concentrate to prepare a combined extract powder.

The present invention relates to a method for fermenting a golden herbal medicine by using a microbial and natural enzyme complex fermentation broth, and then fermenting the fermentation product with a mixture of chimma, dermis, pupa, ginseng and the like, To enhance antioxidant activity against active oxygen and to prevent and improve hyperlipidemia, arteriosclerosis, cerebrovascular disease, memory improvement, and brain cirrhosis.

FIG. 1 is a flowchart showing a process for preparing fermented gold by fermentation aging and drying a golden herbal medicine using lactic acid bacteria and natural enzyme complex fermentation broth according to an embodiment of the present invention.
FIG. 2 is a flow chart showing a process of preparing fermented gold by fermentation aging and drying process of golden herbal medicine using enterobacteria and natural enzyme complex fermentation broth according to another embodiment of the present invention.
FIG. 3 is a flow chart showing a process of preparing fermented gold by fermentation aging and drying a golden herbal medicine using yeast and natural enzyme complex fermentation broth according to still another embodiment of the present invention.
FIG. 4 is a flow chart showing a process of preparing a fermented golden chromosome complex extract powder by mixing fermented gold, chymase, dermis, prawn, and ginseng herbal medicine according to an embodiment of the present invention, followed by extraction, concentration and spraying.
Figures 5a and 5b are comparative HPLC chromatograms of gold and fermented gold prepared according to an embodiment of the present invention.
FIG. 6 is a graph showing an HPLC chromatogram of a chimpanzee added according to an embodiment of the present invention.
7 is a graph showing an HPLC chromatogram of dermis added according to an embodiment of the present invention.
FIG. 8 is a graph comparing active oxygen radical scavenging activities of the gold powder and the fermentation golden raw material prepared according to the embodiment of the present invention by the DPPH method. FIG.
9 is a graph comparing the superoxide radical scavenging activity of the golden powder and the fermentation golden raw material prepared according to the embodiment of the present invention by the xanthine method.
10 is a graph comparing the NO radical scavenging activity of a golden powder and a fermentation golden raw material produced according to an embodiment of the present invention by a grease method.
FIG. 11A is an electron microscopic observation of the growth of neurite outgrowth of PC12 neurons according to the concentration of fermented golden chromosomal complex extract powder prepared according to an embodiment of the present invention. FIG.
FIG. 11B is a graph comparing the growth lengths of neurite outgrowths of PC12 neurons according to the concentration of fermented golden chromosomal complex extract powder prepared according to an embodiment of the present invention.

Figures 1 to 3 illustrate a method for preparing fermentation gold. The fermentation gold manufacturing method of the present invention includes a step of immersing the complex fermentation broth in gold, a fermentation step, an aging step and a drying step.

First, the present invention includes a step of preparing a microbial-enzyme complex fermentation broth by mixing a microbial culture concentrated powder and a fruit enzyme concentrate.

1 to 3, the microorganism-enzyme complex fermentation broth is subjected to the following procedure. The microorganisms used in the present invention are lactic acid bacteria and yeast.

The microorganism powder is inoculated with a microorganism strain in a culture medium, followed by subculture, followed by drying and pulverizing.

In addition, the natural enzyme is obtained by mixing white sugar at an immature month and fermenting it for 6 months, followed by filtration and concentration.

The microbial-enzyme complex fermentation broth is prepared by mixing the previously prepared microbial powder with an enzyme concentrate.

The microorganism strain usable in the present invention may be any one or more of Lactobacillus acidophilus, Streptococcus thermophilus, and Saccharomyces cerevisiae.

In the present invention, the microorganism-enzyme complex fermentation broth is put into gold and fermented for a predetermined time. The gold is used to dry and cut gold medicinal herbs.

The fermentation may be carried out in one to three different ways.

The microorganism-enzyme complex fermentation broth is added so that gold is immersed therein. The gold can be allowed to stand in the immersed state for a predetermined time, preferably at room temperature for a day.

The primary fermentation step is a fermentation step for several days in a fermentation tank. For example, the primary fermentation can be fermented for 5 to 10 days, preferably 7 days, while maintaining 45 to 55 ° C.

The secondary fermentation may be performed by spraying the microorganism-enzyme complex fermentation broth onto the gold and fermenting the fermented product for 5 to 10 days, preferably 7 days, while maintaining the temperature at 45 to 55 ° C. The third fermentation can be further fermented under the same conditions as the second fermentation.

The aging and drying steps may be carried out in various ways, but preferably at a temperature of 40 to 50 ° C, while maintaining a humidity of 40 to 60% and a convection heat cycle, Followed by air drying at 30 to 40 ° C.

The fermentation gold manufacturing method of the present invention is advantageous in that the entire process of fermentation, aging, and drying is continuously performed in the same fermentation vessel, so that the process is simple and the uniformity of the product can be obtained. The method of the present invention includes three stages of fermentation, aging and drying in order to make the fermentation state of the product more uniform and increase the commerciality.

In another aspect, the present invention provides a method for preparing a fermented golden chromosomal complex powder through the steps of preparing a complex extract and preparing a complex concentrate.

The combined extract produces a combined extract from the fermented golden, chunmei, dermis, pupa, and ginseng.

 As the method for producing the above extract, various known methods, solvent extraction method, heated pressure extraction method and the like can be used. For example, the fermented gold, chymus, dermis, pupa, and ginseng may be placed in each extraction container, alcohol solution or water may be added, and the extract may be heated and extracted at a predetermined temperature.

In the present invention, an extract is prepared in three steps. By this method, it is possible to extract by minimizing the loss of the active ingredient. Further, in the solvent extraction method of the present invention, gastrodine, which is an active ingredient of horse chestnut, is hydrolyzed to a parahydroxybenzyl alcohol in a third solvent extraction process to increase extraction efficiency of residual gastrodine, Roxy benzyl alcohol has been reported to restore memory impairment by scopolamine, apomorphine, and cycloheximide.

More specifically, in the step of preparing the complex extract, fermented gold, chymus, dermis, pupa, and ginseng are placed in each extraction container, and the mixture is warmed at 80 to 90 ° C with 50% alcohol solution. The extract is filtered through a filter The tea extract and the 30% alcohol solution were added and the mixture was warmed at 80 to 90 ° C. The extract was filtered through a filter, and purified water was added thereto. The extract was warmed at 80 to 90 ° C and the extract was filtered through a filter And the extract can be combined.

The content of each component can be appropriately controlled in the step of preparing the extract. For example, 200 to 400 parts by weight of gold, 150 to 300 parts by weight of ginseng, dermis, and ginseng can be used as 100 parts by weight of ginseng.

In the concentrate preparation step, the combined extract may be put into a concentrator and concentrated under heating at reduced pressure. For example, after adding the above extracts, they are added to a concentrator and concentrated under reduced pressure of 500 to 700 mmHg at 60 DEG C to obtain a concentrate in the range of 60 to 100 brix.

The combined extract powder can be prepared by adding purified water to the combined concentrate and then spray drying.

The objects of the embodiments of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description .

[Example 1]

First, the golden herbal medicine was dried and cut, and then 1 kg of the golden slice was taken. 5.0 g of Lactobacillus acidophilus strain cultured as a kind of lactic acid bacteria (1 x 10 < 10 > cfu / g) Was mixed with 5 L of purified water, and 2 L of lactic acid bacteria / enzyme complex fermentation broth was immersed in dried gold. Then, the gold was allowed to stand at room temperature for 24 hours, and then fermented for 7 days while kept at 45 to 55 ° C in a fermentation vessel. Then, 1.5 L of the fermented lactic acid bacteria / Then, the third fermentation was carried out in the same manner. After the third fermentation, wet heat and convective heat circulation method using convection heat was maintained for 40 ~ 60% at 40 ~ 50 ℃ for 3 days, and then dried at 30 ~ 40 ℃ to obtain fermentation gold.

[Example 2]

The same procedure as in Example 1 was carried out except that Streptococcus thermophilus strain cultured as a microorganism, which is a kind of enterobacteria, was used (1 x 10 < 6 > cfu / g).

[Example 3]

The same procedure as in Example 1 was carried out except that Saccharomyces cerevisiae strain cultured as a microorganism, a kind of yeast, was cultured in a concentration of 1 x 10 < 6 > cfu / g.

[Example 4]

To 3 kg of fermentation gold of Example 1, 2 kg of ginseng, 2 kg of dandruff, 2 kg of puffed ginger and 1 kg of ginseng were put into an extraction container, 100 L of a 50% alcohol solution was added, and the mixture was heated at 80 to 90 ° C for 8 hours. The extract was filtered through a filter After adding 100 L of a 30% alcohol solution, the mixture was warmed at 80 to 90 ° C for 8 hours. The extract was filtered through a filter and used as a secondary extract. 100 L of purified water was further added thereto, For 10 hours, and the extract was filtered through a filter to obtain a tertiary extract.

The primary, secondary and tertiary extracts were combined and concentrated in a concentrator at 60 ° C under reduced pressure of 500 to 700 mmHg to produce a 65brix concentrate. Purified water was added to the concentrate, followed by spray drying at 185 to 195 ° C to prepare a fermented golden chromatin composite extract powder.

FIGS. 5A and 5B are graphs showing HPLC chromatograms of gold and fermented gold prepared according to an embodiment of the present invention. FIG. 5A and FIG. 5B illustrate results of analysis of fermented gold prepared according to the procedure described above.

  When 50 mg of the fermented golden concentrate was dissolved and dissolved in methanol, the total volume was adjusted to 50 mL, and then 50 mg of the golden concentrate was separately added to 50 mL of methanol And then used as a reference solution.

Acetonitrile: 0.5% phosphoric acid solution (27: 73 v / v) was used as a mobile solvent, and 20 μl of the sample solution was injected. Was a UV detector with a wavelength of 328 nm and a flow rate of 1.0 ml / min. The area of the obtained chromatogram was measured and the content was calculated by the calibration curve method.

 As a result, as shown in FIGS. 5A and 5B, the retention times of the baicalin and baicalein among the gold extracts were 3.50 minutes and 5.30 minutes, respectively. Fig. 5A shows the peak of the golden concentrate, Fig. 5B shows the peak of the fermented golden concentrate, BA in Figs. 5A and 5B shows the bicalin peak, and BE shows the bicalan peak. The content of Baikalin was 8.54% in gold compared to that of fermented gold, and increased to 18.67% in fermentation gold. The content of baicalein was 1.04% in gold and 4.28% in fermentation gold. . This indicates that the extraction efficiency is improved by increasing the water solubility of the active ingredient compared to the fermented gold.

FIG. 6 is a graph showing an HPLC chromatogram of a chimpanzee added in accordance with an embodiment of the present invention. The result of analyzing the chimney ingredient added to the fermented golden chromosome complex extracted powder according to the procedure described above will be described.

When the active ingredient was identified and quantitated by high performance liquid chromatography, purified water was added to 50 mg of the fermented golden Gunma complex extracted powder concentrate to dissolve by heating, and the total volume was adjusted to 50 mL, and then used as a sample solution. Gastrodin (GA) A standard product of hydroxybenzyl alcohol (HBA) and parahydroxybenzaldehyde (HBL) was used as a standard solution for comparison.

The columns were Phenomenex Gemini NX C18 (4.6 × 150 mm, 3 μm pore size) and water / acetonitrile (A / B, v / v) as mobile solvents at 0 min -3% B, 5 min -3% 30 min-60% B, 45 min-60% B, 48 min-25% B, 38 min-40% min, 20% B, and 50 min-3%, and 15 μl of the sample solution was injected. The detector was a UV detector with a wavelength of 220 nm and a flow rate of 0.6 ml / min. And the content was calculated by the calibration curve method.

6, the retention times of gastrodine (GA), parahydroxybenzyl alcohol (HBA) and parahydroxybenzaldehyde (HBL) were 7.86 minutes and 13.79, 24.56 minutes Respectively.

The content of gastrolidine (GA) was 33.5μ, and the parahydroxybenzyl alcohol (HBA) was 193.0μ, which was 5.8 times higher than that of gastroline and the content of parahydroxybenzaldehyde (HBL) was as low as 4.5μ .

 These results indicate that gastrodine is hydrolyzed and converted into parahydroxybenzyl alcohol during the third extraction of the fermented Ganoderma lucidum extract powder. Parahydroxybenzyl alcohol has been reported to restore memory impairment by scopulmine, apomorphine, and cycloheximide.

FIG. 7 is a graph showing an HPLC chromatogram of a dermis added according to an embodiment of the present invention. The result of analyzing the dermis component added to the fermented golden chromosome complex extracted powder according to the procedure described above will be described.

Component identification and analysis by high performance liquid chromatography were carried out by dissolving methanol in 50 mg of the fermented golden chromosomal complex extract powder and dissolving the whole amount to 50 mL, and used as a sample solution, and a standard solution of hesperidin (Hesperidin) was used as a standard solution.

The column used was μ-Bondapak C18 (Waters, ID 3.9 mm × 30 cm, USA), methanol: water (40:60 v / v) was used as the mobile solvent and 20 μl of the sample solution was injected. The wavelength was 280 nm and the flow rate was 1.0 ml / min. The area of the obtained chromatogram was measured and the content was calculated by the calibration curve method.

 As a result, the retention time of hesperidin in the dermis was 2.91 minutes,

The content of hesperidin in fermented golden chromosomal complex extract powder was 16.5%.

FIG. 8 is a graph comparing the activity of the radical scavenging activity of DPPH by the gold powder and the fermentation gold powder prepared according to the example of the present invention. As a comparative measure of the antioxidative effect of the golden concentrate and the fermented golden concentrate, The DPPH (1,1-diphenyl-2-picryl hydrazil) method was applied to measure the antioxidative scavenging activity of the main component and the hydrogel.

The test was carried out by adding 1 mL of a diluted sample solution to 0.5 mL of a 0.2 mM DPPH methanol solution, shaking it for 10 seconds with an agitator, incubating at 20 ° C for 30 minutes, and measuring the absorbance at 517 nm. Here, vitamin C was used as a reference material for comparison.

The size of the activity was determined by the following equation for the concentration of the sample (Free Radical Scavenging Activity, FSC50, ug / mL) and the electron donating ability (EDA%) for DPPH in which the concentration of DPPH (66.7uM) 1 < / RTI >

Here, Sample ABS represents the absorbance of the test liquid to which the sample is added, and Control ABS represents the absorbance of the test liquid to which methanol is added instead of the sample.

As a result, gold was 44.3%, but in fermentation gold, it was 89.8%, showing more than 2 times of active oxygen radical scavenging activity.

FIG. 9 is a graph comparing the superoxide radical scavenging activity of the gold powder and the fermentation gold powder prepared according to the embodiment of the present invention by the xanthine method. The comparative measurement of the antioxidative effect of the golden concentrate and the fermented golden concentrate , And the antioxidative scavenging activities of the main components and the hydrogels were measured by the supernatant radical scavenging activity measurement method using the xylanthin method.

The experiment was carried out at 37 ° C for 30 minutes while adding 100 μL of xanthine oxidase (0.05 U / mL) to a buffer solution (20 mM, pH 7.8) of NBT (Nitro Blue Tetrazoliun) The absorbance at 517 nm was used to determine the NBT reduction capacity.

As a result, gold was 15.4%, but in fermentation gold, it was 58.5%, showing more than three times the superoxide radical scavenging activity.

FIG. 10 is a graph comparing nitric oxide (NO) radical scavenging activity of a gold powder and a fermentation gold powder produced according to an embodiment of the present invention by a grease method. The antioxidative scavenging activity of gold and fermented gold was measured by a grease method (Nitrite oxide) radical scavenging activity measurement method.

The test was carried out by adding a sample to a sodium nitroprusside solution (10 mM), shaking it in a grease solution (0.2% naphthenic ethylenediamine hydrochloride, 2% sulfanilamide, 5% phosphoric acid solution) for 10 seconds with a stirrer, After standing for 2 hours, the absorbance was measured at 570 nm. Here, vitamin C was used as a reference material for comparison.

As a result, it was 10.0% in gold, but 34.0% in fermented gold showed NO radical scavenging activity more than 3 times.

FIGS. 11A and 11B are electron micrographs of the neurite outgrowth of PC12 neurons according to the concentration of fermented golden chromosomal complex extract powder prepared according to the embodiment of the present invention. ) And fermented golden chromosomal complex extract powder, and then measured the change in the length of neurite of PC12 neurons.

The experiment was carried out by mixing 5 ng / mL of low concentration NGF and 10 mg / mL of gold fermented Chunmei combined extract powder at 10, 50, 100, 200 and 500 ㎍ / mL, and culturing for 5 days using RPMI 1640 medium The morphology of PC12 cells was observed by electron microscope and the length of neurite was measured using a micrometer.

 Experimental results showed that the length of the neurites treated with 5 ng / mL of NGF alone was 2.53 μm, while the combination of 50 μg / mL of the fermented golden-chromosome complex extract powder with NGF 5 ng / mL was 124.4 μm, , And the combined yield of 100 ㎍ / mL of fermented golden - chromosomal complex extract powder was 98.8 ㎛.

  On the other hand, the combined length of 200 ㎍ / mL and 500 ㎍ / mL was 37.2 ㎛ and 27.2 ㎛, respectively, and the growth length of neurite was remarkably decreased at high concentration.

As described above, the fermented golden and fermented golden ginseng extract powders fermented and aged with the microbial and natural enzyme complexes according to the embodiments of the present invention showed an increase in the active ingredients of fermented ginseng, bicalin and vicalan, The content of gastrodine, which is an active ingredient, also increased by 5.8 times. In fermentation gold, it shows higher ability of radical scavenging than gold.

The fermented gold and fermented golden ginseng extract powder of the present invention can provide an efficacy for the prevention and improvement of hyperlipidemia, atherosclerosis, cerebrovascular disease, memory improvement, and brain cirrhosis.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be readily apparent that such substitutions, modifications, and alterations are possible.

Claims (4)

Adding microbial-enzyme complex fermentation broth obtained by mixing concentrated microbial powder and fruit enzyme concentrate to gold (Scutellaria radix);
Fermenting the gold; And
And aging the fermented trihydrate for a predetermined period of time and drying the microorganism, wherein the microorganism is selected from the group consisting of Lactobacillus acidophilus, Streptococcus thermophilus, and Saccharomyces cerevisiae ). ≪ / RTI > The method according to claim 1, wherein the fermentation step comprises: 1) fermenting the gold immersed in the microbial-enzyme complex fermentation broth for 2 to 10 days while maintaining 45 to 55 ° C in a fermentation tank; Fermenting the microorganism-enzyme complex fermentation broth for 2 to 10 days while maintaining the temperature at 45 to 55 ° C, and re-injecting the microorganism-enzyme complex fermentation broth and then fermenting the fermented product for 3 to 10 days while maintaining the temperature at 45 to 55 ° C Characterized in that the fermentation process comprises the steps of: Preparing a combined extract of fermented gold, chymus, dermis, pupa, and ginseng according to claim 1 or 2;
A step of preparing a complex concentrate which is obtained by concentrating the complex extract in a concentrator and heating under reduced pressure; And
Adding purified water to the complex concentrate, and spray drying the concentrate to prepare a combined extract powder. [Claim 4] The method according to claim 3, wherein the step of preparing the complex extract comprises: fermenting gold, chymus, dermis, pupa, and ginseng in each extraction container, heating the mixture at 80 to 90 DEG C with a 50% alcohol solution, filtering the extract through a filter The first extract and the 30% alcohol solution were added and the mixture was warmed at 80 to 90 ° C. The extract was filtered through a filter, and purified water was added thereto. The extract was warmed at 80 to 90 ° C and the extract was filtered through a filter And extracting the tea extract.

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