KR20160090956A - Composition for sanitation promotion of oral cavity comprising natural medicinal ingredient extract as effective component for halitosis inducing microoranism and infectious disease of mucous membrane - Google Patents

Abstract

The present invention relates to a composition for enhancing sanitation of an oral cavity comprising a natural medicinal herb extract as an active ingredient. More particularly, the present invention relates to a composition for enhancing sanitation of an oral cavity, comprising extract of at least one selected from Impatiens balsamina L. and Xanthii Fructus and a natural medicinal herb extract of Scutellaria baicalensis Georgi / Paeonia suffruticosa as an active ingredient for halitosis-inducing microorganisms and mucous membrane infection. The composition according to the present invention provides excellent effects of preventing and treating periodontal diseases, preventing dental caries and eliminating halitosis and effects of a natural antibiotic and preservative. Particularly, the composition according to the present invention enhances a synergic effect against halitosis-generating microorganisms and oral cavity mucous membrane infection-inducing microorganisms, an effect of inhibiting plaque formation and an effect of alleviating gingivitis through an adequate combination of naturally occurring material complexes providing antibacterial and antifungal activities. In addition, the composition uses no alcohol to prevent oral cavity dryness. Further, the composition has an effect of preventing oral cavity mucous membrane infection (oral candidiasis) and vaginal infection that children or women frequently experience.

Description

TECHNICAL FIELD The present invention relates to a composition for enhancing oral hygiene comprising a natural medicinal extract as an active ingredient for oral hygiene bacteria and oral mucosal infections. }

The present invention relates to a composition for enhancing oral hygiene comprising a natural medicinal herb extract as an active ingredient for a bad breath inducing microorganism and oral mucosal infectious disease, and more particularly, to a composition for enhancing oral hygiene comprising one kind of extract selected from the group consisting of Balsonia and Chinese persimmon, The present invention relates to a composition for enhancing oral hygiene comprising a medicinal extract as an active ingredient for a bad breath inducing microorganism and an oral mucosal infection. The composition enhances the antimicrobial activity, plaque formation inhibition effect and gingival inflammation relaxation effect on halitosis causing bacteria and oral mucosal infectious bacteria by proper mixing of the complexes of natural materials that express antibacterial and antifungal activity .

In ancient times, ancient Chinese used various herbal medicines for the prevention or treatment of oral diseases. Especially ancient Chinese used Inhin Tong, a medicinal herb for gingival inflammation and gingival bleeding, The Enishi used honey and the like. In the Middle Ages, opium and rose oil were used. In the 14th and 15th centuries, white wine and roasted salt were used. In the Asian region, Chinese medicine was used for prevention and treatment of various oral diseases.

As an effort to prevent periodontal diseases and tooth decay and halitosis, antibacterial agents such as chlorohexidine, quaternary ammonium compounds such as cetylpyridinium chloride, triclosan, and xanthine, which can sterilize bacteria present in the oral cavity, Antibiotics such as penicillin, enzyme inhibitors such as fluoride compounds such as sodium fluoride and sodium monofluorophosphate and dextranase which inhibit the demineralization of teeth or accelerate remineralization to prevent the occurrence of cavities have been used variously, Similarly, herbal medicine extracts have been studied in various ways and applied to products.

For example, in Japanese Patent Laid-Open No. 55-120,509, herbal medicine extracts such as bone cucumber, mulberry leaf, oilseed oil, lotus root, and lavender are used. In Japanese Patent Publications 57-5641 and 57-85319, 87-268613 and 60-75418 in Japanese Patent Application No. 60-75418 in order to obtain a bacteriostatic effect in the oral cavity by blending sage or rosemary.

However, all of the ingredients used in the above-described prior arts have a problem that the continuous oral antimicrobial inhibitory effect is so weak that the effect of prevention and treatment of periodontal disease, prevention of tooth decay and removal of bad breath is weak and the usability is poor when applied to a product have.

Cetylpyridinium chloride (CPC) is a quaternary ammonium cation used for mouth cleaners, toothpaste, mouth spray, nasal spray, etc., which has a relatively low human side effect. It has antibacterial effect against bacteria and microorganisms, plaque) and reduce gingivitis (Source: www.wikipedia.com ). In this regard, US Patent No. 6,861,047 discloses toothpastes and mouthwashes comprising CPC and sodium dihydroacetate, and Korean Patent Application No. 10-2001-0045337 discloses that seeds such as bean sprouts, Disclosed is a method of preventing CPC from being infested by immersion, spraying or irrigation. In addition, international application PCT / US2000 / 011425 discloses four components of CPC and behentolinium methosulfate and behentrimonium methosulfate and stearyl alcohol quaternium and Cetrimonium chloride, A sanitary napkin, and a sanitary napkin product selected from the group of wipes products. However, CPC begins to freeze at about 15 ° C when dissolved in purified water, and recrystallization starts at about 13 ° C. Therefore, there is a problem in commercialization when purified water is used as a solvent.

In general, tooth decay and periodontal disease are important diseases that occur in teeth and surrounding tissues. The cause of tooth decay is known to be caused by plaque (or plaque), which is a metabolite of bacteria and bacteria residing in the oral cavity. The cause of periodontal disease is also known as the metabolism and metabolism of specific bacteria in the tooth and gum .

Oral dryness is a disease in which saliva is secreted less than 0.1 mL per minute and can not decompose food waste in the mouth and can not inhibit bad bacteria. People with dry mouth have a smell in their mouth because they do not act as a saliva. However, the use of oral cleaning agents to mask the odor of dry mouth patients is rather counterproductive.

Oral cleansers are largely divided into alcohol-containing products and non-alcoholic products. The problem is alcohol-containing oral cleansers. Using alcohol-containing mouthwashes will leave alcohol in your mouth. This reduces the saliva secretion, making the mouth dry more quickly, and bacteria that harm oral health reproduce quickly. The more bacteria, the worse the bad breath.

There are many people who use mouthwash to prevent tooth decay. However, people with dry mouth can use alcohol-containing mouthwashes, which can cause bad breath. Dental specialists say, "People with dry mouth should not use mouthwash with alcohol." If oral hygiene is an issue with extraction or oral surgery, it is advisable to use mouthwash only for 1 to 2 weeks "Is recommended.

Oral candidiasis is a fungal disease called Candida albicans that grows in the oral mucosa and is often called throat. Candida is a kind of fungus that is always present in the mouth, and it is easy for the elderly who are weak in resistance or infants of malnutrition. It is a kind of infectious disease. It appears as a fine white deposit on the red spot on the oral mucosa, or as a soft, raised white spot, like condensed milk. When rubbed with cotton or gauze, the membrane is peeled off, followed by a red mucosal surface. When food comes in here, it causes pain and bitter pain. Such oral mucosal infections caused by Candida albicans are separated from the mucous membranes of healthy individuals, which are caused by infiltration into the oral mucosa when the immune system weakens. Causes are those that are caused by diseases with malnutrition such as malnutrition, diabetes, rheumatoid arthritis, and are often caused by weakness of immunity such as long-term use of antibiotics, corticosteroids and immunosuppressants. In the case of oral candidiasis, the mother is often infected. In the elderly, it is often reported that the denture is not cleaned properly or under the dentures.

Streptococcus mutans is a major factor, although many species are known to form microorganisms. Streptococcus mutans secretes an enzyme called glucosyltransferase (GTase) in the outer layer of cells or on the surface of cells to produce glucose and fructose from sucrose as a substrate, To form an insoluble polymer, glucan. This glucan causes various microorganisms in the oral cavity to attach to the tooth surface to form a bacterial membrane, which is called plaque (or plaque).

When lactobacillus including Streptococcus mutans is propagated in the prage, lactic acid is formed by using fructose as a carbon source. When this lactic acid is captured in the adherent glucan, it is concentrated with a high concentration of acid to dissolve calcium ions in the teeth and as a result enamel (J. Periodontol. 63: 322-331, 1992).

In order to prevent such tooth decay and gum disease, it is necessary to inhibit or sterilize the growth of Streptococcus mutans and Poppyromonas juniperis. Examples of substances having such functions include penicillin, erythromycin, tetracycline, and spiramycin There are antibiotics, especially triclosan, a non-ionic disinfectant. Korean Patent Publication No. 1999-0079220 discloses an oral composition using triclosan, and Korean Patent No. 10-2006-0089890 suggests a method of increasing the residual effect of triclosan in the oral cavity.

Antibiotics, however, have side effects such as the development of resistant bacteria, hypersensitivity reactions, and gastrointestinal disorders. In particular, antibacterial agents such as chlorohexidine have the effect of inhibiting the formation of cavities, but they are known to have side effects such as destruction of the ecosystem in the mouth, have. Therefore, studies on antimicrobial and fungicides utilizing natural products that have been used for a long time are being actively pursued as preparations which can minimize or supplement the side effects exhibited by these chemical agents.

The oral cleanser compositions may include solubilizing agents, wetting agents, solvents, fragrances, sweeteners, preservatives, buffers, pigments, and the like. However, as discussed below, it should be noted that many of the ingredients present in most current mouthwashes marketed in the country may cause side effects.

If mouthwash is applied after brushing, mucous membrane is stimulated by alcohol and infection of fungus such as oral candidiasis which is caught in mouth by long-term use can come.

On the other hand, in the case of elderly people who have dry mouth or dry mouth frequently, it is better not to use oral cleansers that are currently marketed with alcoholic ingredients after tooth brushing because the symptoms may become worse.

There have also been many reports of adverse effects of alcohol contained in mouthwashes on the human body as they are oxidized to acetaldehyde in oral cavity [Lachenmeier DW. Safety evaluation of topical applications of ethanol on the skin and inside the oral cavity. J Occup Med Toxicol. 2008; 3: 26]. Especially for children under the age of 14, alcohol is strongly recommended not to use mouthwashes containing alcohol [Lachenmeier DW. Safety Assesment of Alcohol-Containing Mouthwashes and Oral Rinses. SOFW-Journal, 134, 10, 2008].

The following ingredients are frequently used in conventional mouthwashes.

The solubilizer gives a cooling sensation when used in the product, and it can dissolve fragrance or preservative that does not dissolve in water. Alcohols, polyoxypropylene copolymerized polymers, polyoxyethylene hydrogenated castor oil, high alkyl acetates, and polyoxyethylene derivatives of sorbitan fatty acid esters, and from these, at least one selected from 20 to 60 By weight, preferably 5 to 20% by weight.

Wetting agents serve to impart stability, sweet taste and slight viscosity of the product. Glycerin, sorbitol solution, polyethylene glycol, etc. are used alone or in admixture of two or more kinds in an amount of 20 to 60% by weight, preferably 20 to 50% by weight.

As the preservative, 0.05 to 0.25% by weight of methylparaben and propylparaben are used alone or in combination. In Korea, the KFDA has limited use, but the following side effects have been reported when overexposed. It reacts with oxalic acid to stimulate central nerve paralysis, hemorrhagic gastritis, liver adverse effects, carcinogenicity, chromosomal aberration, eye, and skin mucosa. Parabens such as methylparaben, ethylparaben, and propylparaben are widely used as preservatives for cosmetics. They are classified as harmful substances because they are known to cause endocrine disruption, skin aging, and breast cancer when they are absorbed into the human body.

As the pH adjusting agent, 0.02 to 1.5% by weight of phosphoric acid, sodium phosphate, citric acid, sodium citrate, succinic acid, sodium succinate, tartaric acid and sodium tartrate are preferably used singly or in combination of two kinds.

Examples of the material constituting the perfume include aromatic materials used in general toothpaste and materials used for food flavoring such as peppermint oil, menthol, spearmint oil, carbohydrate, anise oil, anthol, eucalyptus oil, clove oil A flavor enhancer, a wintergreen oil system, a methyl salicylate, a cinnamon oil system, a cinnamic aldehyde and other perfume ingredients are used, and the material constituting the perfume is mixed with one or more of the above ingredients in an amount of 0.1 to 2.0 wt. %, Preferably 0.5 to 1.5 wt%.

Accordingly, the present inventors conducted experiments using various natural medicinal plant extracts in order to prepare oral compositions excellent in the effect of suppressing bad breath, and found that the extracts of Balsonia, Chinese persimmon and Chinese medicinal herbs extracts of bad breath causing substances and odor causing bacteria The present inventors completed the present invention by confirming that it is possible to maximize the antibacterial activity and exhilaration by mixing the natural materials used in the conventional products with an appropriate amount.

It is an object of the present invention to provide a composition for promoting oral hygiene which is excellent in antibacterial activity against oral microorganisms, oral anti-inflammatory activity and ability to remove bad breath inducing substances, and thus has an excellent effect of inhibiting bad breath.

Specifically, it is an object of the present invention to provide an oral composition for suppressing bad breath by suppressing and eliminating malodor causing substances that cause bad breath, and more particularly, to provide an oral composition which inhibits halitosis, And a composition containing the mixture of the substance groups having an optimal composition ratio as an active ingredient, and to provide an oral composition for removing harmful bacteria in the oral cavity.

In order to achieve the above object, the present invention provides a composition for promoting oral hygiene comprising an extract of one kind selected from the group consisting of Balsam and Chinese persimmon, and a natural / medicinal herbal extract of gold / woody as an active ingredient for a bad breath inducing bacteria and an oral mucosal infection do.

Hereinafter, the present invention will be described in detail.

In the present invention, what is described as "golden / woody" is meant to include both gold and woody.

In the composition for enhancing oral hygiene of the present invention, it is preferable that the composition contains all of the balsam, the sorghum, and the golden / herring extract, and the composition is at least one selected from the group consisting of copper sulfate, betaine and N- Is further preferably contained.

In addition, in the composition for enhancing oral hygiene of the present invention, it is preferable that the balsam bark, the leaf extract and the golden / bark extract are contained in the range of 0.001 to 5.0 wt.% Of the total composition weight, It is more preferable that the extract, copper sulfate, betaine, and N-oxide are contained in the range of 0.001 to 5.0 wt% of the total composition, and the balsam, the genus, the golden / It is most preferable to contain the composition in the range of 0.01 to 1.0 wt% of the weight of the composition in view of the effect or the stability of the preparation.

When the content of each medicinal ingredient is 0.001% by weight or less, the medicinal effect is hardly exhibited. When the medicinal ingredient content is 5.0% by weight or more, precipitates may be formed or the color of the preparation solution may be changed.

In addition, in the composition for enhancing oral hygiene of the present invention, the composition may be selected from the group consisting of sodium fluoride, glycerin, citric acid, sodium citrate, xylitol, sodium saccharin, poloxamer 407, combination mint flavor, cocamidopropyl betaine, _4. 5H ₂ O, balsam, chrysanthemum, golden / herring extract and purified water.

In addition, in the composition for enhancing oral hygiene of the present invention, the betaine may be at least one selected from the group consisting of alkyl-N-betaine, alkyl-N-sulfobetaine, acyl-N-betaine, alkyl N-substituted aminopropionic acid and alkylimidazolinium And betaine, and it is more preferable that the betaine has a structural formula of the following formula (1) or (2).

(화학식 1)

(Formula 1)

(화학식 2)

(2)

In the above Chemical Formulas 1 and 2, R is a radical of a higher alkyl group of 10 to 18 carbon atoms selected from the group consisting of decyl, undecyl, lauryl, tridecyl, myristyl, cetyl, stearyl, isostearyl and oleyl .

In the composition for enhancing oral hygiene of the present invention, the amine oxide may be selected from the group consisting of alkyl-N, N-dimethylamine oxide represented by the following formula (3), alkyl-N, N-dihydroxyethylamine oxide represented by the formula Acylamide t-amine oxide, and the amine oxide is at least one selected from the group consisting of decyl-N, N-dimethylamine oxide, lauryl-N, N-dimethylamine oxide, stearyl- Dimethylamine oxide, oleyl-N, N-dimethylamine oxide, coco-N, N-dihydroxyethylamine oxide, cetyl-N, N-dihydroxyethylamine oxide, oleyl- N, N-dihydroxyethylamine oxide and oleyl-N, N-dihydroxyethyl-amine oxide are more preferred.

(화학식 3)

(Formula 3)

(화학식 4)

(Formula 4)

(화학식 5)

(Formula 5)

The composition for enhancing oral hygiene of the present invention is a natural antimicrobial agent which is not toxic to humans and is used as a synthetic antimicrobial agent for which side effects have been reported. In addition, a non-synthetic preservative that does not use alcohol that causes dryness in the oral cavity, .

In addition, the compositions for enhancing oral hygiene of the present invention may be prepared into pharmaceutically acceptable non-toxic salts and solvates by conventional methods in the art.

Pharmaceutically acceptable salts include acid addition salts formed by free acids. The acid addition salt is prepared by a conventional method, for example, by dissolving the compound in an excess amount of an acid aqueous solution, and precipitating the salt using a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile. The same molar amount of the compound and the acid or alcohol (e.g., glycol monomethyl ether) in water may be heated and then the mixture may be evaporated to dryness, or the precipitated salt may be subjected to suction filtration.

Pharmaceutically acceptable salts of the compounds of the present invention include, unless otherwise indicated, salts of acidic or basic groups that may be present in the compounds of the present invention. For example, pharmaceutically acceptable salts include the sodium, calcium, and potassium salts of the hydroxy group, and other pharmaceutically acceptable salts of the amino group include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, But are not limited to, salts known in the art such as, for example, salts of phosphoric acid, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate (mesylate) and p- toluenesulfonate (tosylate) And can be manufactured through a manufacturing method or a manufacturing process.

The pharmaceutical compositions containing the balsameris, genus, and golden / herring extract of the present invention as active ingredients can be formulated into formulations such as powders, suspensions, emulsions, syrups and aerosols according to the conventional methods.

The pharmaceutical composition of the present invention containing the balsam or the extract of Aspergillus oryzae as an active ingredient may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

Examples of carriers, excipients, and diluents that can be contained in the composition containing the above-described balsamic shark, Chinese lobster, golden / mulberry extract as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol , Starch, acacia rubber alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and Mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

The preferred amount of the composition of the present invention can be suitably selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably used at a daily dose of 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg. It can be used once a day or divided into several times. The amount used is not intended to limit the scope of the invention in any way.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so important, but is generally selected in the range of 0.001 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

First, the characteristics of each component contained in the oral composition of the present invention will be described.

≪ Herbal medicine materials and extraction method >

The extracts were prepared by filtration after stirring for 3 days at room temperature (15 ~ 25 ℃) using 70% ethanol extraction method.

The contents of indicator substances and heavy metals were confirmed based on the test method of Korea Food and Drug Administration.

(1) Balsam Extract (鳳仙花, Garden Balsam)

It is a year early in the year, growing about 60 ~ 80㎝ in height. The stem stands upright, and the nodule of the lower part stands out by the flesh quality and the red light turns. Leaf length is 8 ~ 10㎝, lanceolate and both ends are narrow. There is a stump on the edge of the leaves, and it is off. Flowers bloom in July ~ August in leaves (axilla) 2 ~ 3, and vary in purple, white, red, and variegated. The fruit is oval and oval with hairs. When ripe, it bursts and yellowish brown seed pops out.

Balsam extract is a yellowish brown liquid with a characteristic odor and a slight bitter taste. Balsonic shoots were obtained by extracting with water ethanol at room temperature. Quercetin was the main component.

(2) The cherry tree (蒼 耳 子, Xanthii Fructus)

It is a ripe fruit of Xanthium strumarium Linne (Asteraceae Compositae). Fusiform or oval, 10 to 15 mm in length and 4 to 7 mm in diameter. There is a peculiar smell and the taste is slightly bitter. The main ingredients are Xanthostrumarin, Linoleic acid and Vitamin C. The pharmacological action is antimicrobial action by Xanthatin, treatment of sinusitis and rhinitis, treatment of skin disease is known.

(3) Moutan Root Bark

Paeonia suffruticosa is a root shell of Andrews (peonies and Paeoniaceae). Its shape is cylindrical or semicylindrical, 5 ~ 20㎝ in length, 0.5 ~ 1.2cm in diameter, and 0.1 ~ 0.4cm in thickness. There is a peculiar smell and the taste is a bit spicy. The main ingredients are Paeoniflorin, Oxypaeoniflorin, Paeonol, Paeonolide, Paeonoside, and Tannin. Antimicrobial effects, antioxidant, anti-inflammatory, antiviral and anticonvulsive effects are known as pharmacological actions.

(4) Golden (黄 芩, Scutellaria Root)

The spiny grass belonging to Lamiaceae and Labiatae is the root of Scutellaria baicalensis Georgi as it is or has been removed. The shape is circular, semi-tubular or flat, 5 ~ 25㎝ in length and 5 ~ 30㎜ in diameter. There is little smell and taste a little bit. The main ingredients are Baicalein, Baicalin, Wogonin, Wogonoside, Neobaicalein, and β-Sitosterol. The antimicrobial effect, anti-cancer, anti-inflammation, ejaculation, hypotension, and diuretic effect are known as pharmacological actions.

(5) copper sulfate

Vitar pigment, used as a coloring agent for vegetables and fruits, such as storage products and kelp.

The formula is CuSO ₄ .5H ₂ O. It is a blue crystal, lump or bluish white crystalline powder. It is soluble in water and slightly soluble in ethanol. The aqueous solution is blue and acidic during hydrolysis. When left in the air, it gradually becomes enriched, loses crystals and discolors to white. When heated, it becomes anhydrous at 25 ℃. Color is colorless and it becomes copper oxide when heated to 650 ℃. It is known that it acts on the cell walls of algae and fungi and exhibits antibacterial and antifungal properties.

(6) Xylitol

Xylitol can help relieve symptoms by stimulating salivation when patients with dry mouth develop dry mouth due to aging. At this time, xylitol should be kept in the oral cavity for a long period of time.

Xylitol inhibits the growth of hemolytic streptococci including Pneumoniae, and steadily ingesting xylitol inhibits the growth of pathogenic bacteria in the respiratory and digestive tracts. It is also effective in preventing childhood otitis media.

(7) C31G

Certain mixtures of amines are known to be effective antimicrobial agents against gram negative and gram positive bacteria. (A) alkyl-N-betaine, alkyl-N-sulfobetaine, acyl-N-betaine, alkyl N- substituted amino propionic acid or alkyl imidazolinium betaine and (b) It is known that certain mixtures of dimethylamine oxides, alkyl-N, N-dihydroxyethylamine oxides or acylamide t-amine oxides can be used for skin germ removal, purification and deodorization (see, for example, EB Michaels US Pat. No. 4,183,952; US Pat. No. 4,075,350 to EB Michaels and US Pat. No. 4,107,328 to EB Michaels). These compositions are also useful for prolonged inhibition of body odor. Mixtures of the same active ingredients are also known to enhance oral hygiene by eliminating oral microorganisms and inhibiting the formation of jigs (US Pat. No. 4,839,158 to E. B. Michaels). This type of composition is also known to be an effective fungicide (Corner, AM, Antimicrobial Agents and Chemotherapy, 32 (3): 350-353 (1988, 3); Corner, AM, The Journal of Clinical Dentistry, Vol. 2 (3): 35-38 (1990)). (See also U.S. Pat. Nos. 4,075,350, 4,107,328, 4,183,952 and 4,839,158, issued to E. B. Michaels). It is also known that non-ionic surfactants which possess ether or amide bonds between the hydrophilic and hydrophobic sites of the molecule rapidly inactivate the infectivity of herpes simplex viruses I and II (Asculai, SS, an antimicrobial agent Chemotherapy Vol. 13: P 686-690 (1978)). The composition used in the process of the invention consists of a mixture of betaines and amine oxides. The betaine used in the present invention may be selected from (a) alkyl-N-betaines, alkyl-N-sulfobetaines, acyl-N-betaines, alkyl N-substituted aminopropionic acids, alkylimidazolinium betaines, And mixtures thereof. Typically, they have two lower alkyl groups on the nitrogen atom. Most effectively they have two methyl substituents on the nitrogen atom. The amine oxides used in the present invention are selected from the group consisting of (b) alkyl-N, N-dimethylamine oxides, alkyl-N, N-dihydroxyethylamine oxides or acylamide t-amine oxides and mixtures of two or more thereof do. Typically, the betaine and amine oxide components are present in a molar ratio of 1: 5 to 5: 1, preferably in a molar ratio of 1: 1. Alkyl-N-sulphobetaine, acyl-N-betaine, alkyl-N-substituted amino propionic acid and alkyl imidazolinium betaine ( Each of which is also referred to as cocoamphoacetate) has the following structure: in an amount of 0.001 to 1.0% by weight based on the total weight of the composition.

Wherein R is a radical such as a higher alkyl group of 10 to 18 carbon atoms such as decyl, undecyl, lauryl, tridecyl, myristyl, cetyl, stearyl, isostearyl or oleyl.

Examples of amine oxides include decyl-N, N-dimethylamine oxide, lauryl-N, N-dimethylamine oxide, stearyl-N, N-dimethylamine oxide, oleyl- N, N-dihydroxyethylamine oxide, N, N-dihydroxyethylamine oxide, cetyl-N, N-dihydroxyethylamine oxide, oleyl- N, N-dihydroxyethyl-amine oxides, and mixtures thereof.

The composition for enhancing oral hygiene according to the present invention maximizes the antibacterial and antifungal activities through mutual synergistic action of the pharmacological components, thereby providing excellent effects in preventing oral mucosal infections (oral candidiasis) and removing bad breath In addition, the composition can be preserved for a long period of time without adding a human body harmful preservative. In addition, by not using alcohol that causes side effects such as dry mouth, it can reduce concern about oral hygiene and health safety of the general public.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings. However, it should be understood that the following embodiments are provided so that those skilled in the art may understand the present invention without departing from the scope and spirit of the present invention. It is not.

≪ Example 1 > Preparation of a saliva composition 1

The compositions of the composition were prepared in various composition ratios according to the contents and contents shown in Table 1 below.

Specifically, cocamidopropyl betaine, lauramin oxide, poloxamer 407, and glycerin were dissolved in 10 g of purified water and completely dissolved therein, followed by complete dissolution by adding Baejonhwa, Changsai, golden / herbaceous and combination mint flavor. To the completely dissolved solution, CuSO ₄ .5H ₂ O, sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added.

≪ Example 2 >

A vial solution composition was prepared in the same manner as in Example 1 above. However, it was not added only ganoderma and gold / mulberry extract.

≪ Example 3 >

A vial solution composition was prepared in the same manner as in Example 1 above. However, only balsam and golden / herring extracts were not added.

<Example 4>

A vial solution composition was prepared in the same manner as in Example 1 above. However, there was no addition of balsam and alternate extracts.

&Lt; Comparative Example 1 &

Cocamidopropyl betaine, lauramin oxide, poloxamer 407, glycerin, and purified water (10 g) were added and stirred to complete dissolution, followed by complete dissolution with a combination mint flavor. To the completely dissolved solution, CuSO ₄ .5H ₂ O, sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added.

&Lt; Comparative Example 2 &

Cocamidopropyl betaine, lauramin oxide, poloxamer 407, glycerin, and purified water (10 g) were added and stirred to complete dissolution, followed by complete dissolution with a combination mint flavor. Sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added to the completely dissolved solution.

&Lt; Comparative Example 3 &

Poloxamer 407, glycerin, and purified water 10 g were added and stirred to complete dissolution, followed by complete dissolution of the combined mint flavor. To the completely dissolved solution, CuSO ₄ .5H ₂ O, sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added.

&Lt; Comparative Example 4 &

The product of L company sold on the market and its composition is as shown in Table 1 below.

Specifically, 5 g of alcohol, Poloxamer 407, glycerin and 10 g of purified water were added and stirred to complete dissolution, followed by complete dissolution in combination of mint flavor. Sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added to the completely dissolved solution.

&Lt; Comparative Example 5 (blank control group) >

Poloxamer 407, glycerin, and purified water 10 g were added and stirred to complete dissolution, followed by complete dissolution of the combined mint flavor. Sodium fluoride, citric acid, sodium citrate, xylitol, sodium saccharin and other purified water were added to the completely dissolved solution.

Table 1 summarizes the components and contents described in the above Examples and Comparative Examples.

Ingredients Example Comparative Example One 2 3 4 One 2 3 4 (L Company
product) Sodium fluoride 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 Citric acid 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.02 Sodium citrate 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.10 Xylitol 1.00 1.00 1.00 1.00 1.00 1.00 1.00 - Sodium saccharin 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Poloxamer 407 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.08 Combination mint  incense 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.15 Cocamidopropyl  Betaine (30%) 0.23 0.23 0.23 0.23 0.23 0.23 - - Raoul
Amine oxide
(30%) 0.15 0.15 0.15 0.15 0.15 0.15 - -

5H

O CuSO

5H

O 0.16 0.16 0.16 - 0.16 - 0.16 - Balsam extract 0.40 0.80 - - - - - - Ganoderma extract 0.40 - 0.80 - - - - - Golden / Root Extract 0.60 - - 0.60 - - - - Ethanol (95%) - - - - - - - 5.00 Purified water to 100.0 to 100.0 to 100.0 to 100.0 to 100.0 to 100.0 to 100.0 to 100.0

(Unit: wt%)

&Lt; Test Example 1 >

&Lt; 1-1 >

The strains used in the experiments were purchased from the Center for Microbial Resources, Korea Research Institute of Bioscience and Biotechnology. Specifically, the present inventors have found that Streptococcus mutans KCTC 3065, a cause of cavity and bad breath, Candida albicans KCTC 7965, a cause of oral mucosal infections, and Aspergillus, Aspergillus fumigatus KCTC 26426 was used.

Streptococcus ( S. mutans ) is the most common oral bacteria and is a major causative agent of halitosis and dental caries. Streptococcus produces glucosyl transferase, which produces insoluble glucan from sugar It attaches to the tooth surface and through proliferation forms a dental plaque as a sticky, colorless substance. Of the tartar, 70% are bacteria, and 30% are sugar, enzymes, and lactic acid. The abortion releases calcium and phosphorus from the tooth enamel layer, degrades the hydroxyapatite, and erodes the enamel layer. Bacteria enter the teeth and induce cavities, which usually takes one to two years.

Candida albicans is the most common causative organism of fungal infections. It is present in almost all natural systems such as animals, plants, soil and marine, and there are about 20 Candida species isolated from humans. Candida albicans is the most common Is a human body. Candida albicans, known as the major pathogen of Candidiasis, is a species that accounts for 60% of Candida spp. Causing disease. It is parasitized as a persistent bacterium in humans and other warm-blooded animals. When host immune status deteriorates, It is an opportunistic pathogen.

Oral candidiasis accounts for more than 50% of the total, and oral candidiasis is also known as thrush. It is characterized by the appearance of white spots and ulcers in the mucous membranes of the mouth, tongue, and throat, and it is known to occur in denture users, mainly in patients with weakened immunity due to antibiotics, chemotherapy, and AIDS.

A. niger, A. fumigatus, and A. flavus cause Aspergillosis. Healthy people have no infectivity, so they are pure opportunistic fungi, but they are highly infectious for immunosuppressants and long-term antibiotics. In addition, Aspergillus (Aspergillus) are distributed worldwide and causes an infection only mycelial form. The main infection infiltration pathway may be respiratory tract respiratory aspergillosis due to inhaled conidia.

Table 2 and Table 3 below show the characteristics of the strain and the medium composition for culturing, respectively.

List of test strains for antimicrobial testing Strain number Strain name badge Incubation temperature 3065 Streptococcus mutans Brain heart infusion 37 ℃ 7965 Candida albicans Yeast Malt 25 ℃ 26426 Aspergillus fumigatus PDB (Potato Dextrose Broth) 25 ℃

Medium composition for test strain culture Culture protocol Brain Heart Extract Broth 37.0g / L + Distilled Water 1.0L + Agar 15.0g / L Yeast Malt 23.0g / L + Distilled Water 1.0L + Agar 15.0g / L PDB 27.0 g / L + distilled water 1.0 L + agar 15.0 g / L

<1-2> Antimicrobial test using disk diffusion method

Each strain was adjusted to 2.0 x 10 &lt; ⁷ &gt; cfu / ml in a solid medium, and 100 [mu] l was added thereto, followed by spreading using a spreader. 100 μl of each of Examples 1 to 4 and Comparative Examples 1 to 4 was dripped onto a sterilized paper disc (Ф10mm, Advantec, Japan), followed by drying and placing the strain on the smear medium. S. mutans (37 ℃) was cultured for 48 hours and C. albicans (25 ℃) and A. fumigatus (25 ℃) were cultured for 24 hours. After the culture, the diameter (mm) of the transparent ring around the paper disk was measured. The results are shown in Table 4 below.

Test bacteria Example Comparative Example One 2 3 4 One 2 3 4 (L Company
product) S. mutans 30 15 17 15 13 11 0 0 C. albicans 27 11 0 0 0 0 0 0 A. fumigatus 24 15 13 11 0 0 0 0

(Unit: mm)

The results of Table 4 can be summarized as follows.

Those containing natural extracts were divided into Examples and those not contained were divided into Comparative Examples.

First, the antibacterial activity against Streptococcus mutans was divided into the examples and the comparative examples, and the results of the examples showed excellent results. These results showed that when the proper amount of extracts of natural medicinal herbs such as balsam extracts, gerbera, golden, The antibacterial activity of C. albicans was compared in Examples 1 to 4. Examples 2 and 3 show the antibacterial activity of C. albicans depending on the presence or absence of the balsam extract. The extract of chrysanthemum extract (Example 3) has activity against bad breath inducing bacteria, but shows no antibacterial activity against oral mucosal symptoms. In addition, comparing Example 1 with Example 2, the antibacterial activity of C. albicans did not increase even when the content of the balsam extract was doubled, but the mixture with the extract was more active. Comparative Examples 1, 2 and 3 showed antibacterial activity depending on the presence or absence of C31G (for example, the same equivalent mixture of cocamidopropyl betaine and raulamine oxide) and copper sulfate. Comparing Comparative Example 3 with 1 and 2, there was no antibacterial activity against bad breath inducing bacteria in the absence of C31G (equivalent equivalent of betaine and amine oxide).

The activity against the strain of Aspergillus fumigatus was not observed when the copper divalent ion was present alone (Comparative Example 3), but when the gold / mulberry extract was added (Example 4) (Examples 2 and 3), and the activity was maximized when each natural product was added in a ratio of 1.5: 1: 1.

Comparative Example 4 is one of commercially available products of Company L. The solution containing 5% alcohol is known to have activity against bad breath inducing bacteria. However, as a result of testing the antimicrobial activity through the paper disk method as in the present invention, no activity was observed. That is, it can be seen that the composition is not a composition in which the antimicrobial activity is expressed in the absence of alcohol as pursued by the present inventors.

&Lt; Test Example 2 > Measurement of deodorizing effect

A blank control (Comparative Example 5) in which neither of the above Examples 1 to 4 and Comparative Examples 1 to 4 and C31G, natural extract, or mint flavor was added (Comparative Example 5) And the odor inhibitory effect on the saliva composition was measured as follows. Fifty men (15 males, 35 females) without dental caries were selected and 1.0 g of fresh gum was masticated. After 5 minutes, the mouth was washed with water, and then the degree of bad breath was measured with a halide meter And the initial value was set for each individual. The test group was divided into 5 groups (3 males and 7 females) and fingered with each of the toothpastes prepared in Examples 1 to 4 and Comparative Examples 1 to 4 and negative control group (Comparative Example 5) for 2 minutes After 30 minutes, the bad breath was measured again with a breath analyzer, and the final value was compared with the above initial value. The method of using the breathalyzer is as follows. Before the measurement, the mouth was closed for 2 minutes, and the breath of the choromone was allowed to be maintained. In the measurement, the straw was kept in the mouth for about 15 seconds while being put in the mouth for about 1.5 seconds. At this time, the straw was kept from touching the tongue and the lips were maintained at a distance of about 3 to 4 mm. Table 5 shows the odor suppression values (average value of 10 persons) of each test group obtained by measuring the measured values (in ppb units) of the breath analyzer obtained from the respective subjects. The calculation formula of the halitosis suppression rate is shown in the following Equation 1.

Bad breath inhibition rate (%) = 100 x (final value - initial value) / initial value <Formula 1>

Example Comparative Example One 2 3 4 One 2 3 4 (manufactured by L company) Blank Average odor suppression 93 81 80 83 57 46 45 79 20

(unit: %)

As shown in Table 5, the halothane produced after raw garlic chewing showed a suppression rate even in a negative control (no composition containing alcohol in Comparative Example 5, product L) containing no active ingredient, but less than about 20% . The increase in halitosis inhibition rate is proportional to the intensity of the activity against Streptococcus mutans . When mixed with C31G, a combination of mint series, a natural medicinal substance having antimicrobial activity and a copper sulfate solution as a badodurative substance remover, It was confirmed that halitosis could be suppressed more efficiently by the synergistic effect of.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, This is possible.

Claims (11)

A composition for enhancing oral hygiene comprising one kind of extract selected from the group consisting of Bongsulhwa and Changsik, and a natural / medicinal herbal extract of gold / woody as an active ingredient for bad breath inducing bacteria and oral mucosal infections.
The composition for promoting oral hygiene according to claim 1, wherein the composition contains both a balsam sword, a sorter, and a golden / herring extract.
The composition for promoting oral hygiene according to claim 1 or 2, wherein the composition further comprises at least one selected from the group consisting of copper sulfate, betaine, and N-oxide.
The composition for promoting oral hygiene according to any one of claims 1 to 3, wherein the balsameris, ginseng and golden / ginseng extract are contained in an amount of 0.001 to 5.0% by weight of the total composition.
4. The composition for enhancing oral hygiene according to claim 3, wherein the balsam sausage, sorter, golden / herring extract, copper sulfate, betaine and N-oxide are contained in an amount of 0.001 to 5.0% by weight of the total composition.
6. The composition for promoting oral hygiene according to claim 5, wherein the balsam ation, ginseng, golden / herring root extract, copper sulfate, betaine and N-oxide are contained in an amount of 0.01 to 1.0 wt% of the total composition weight.
The composition of claim 1 wherein said composition is selected from the group consisting of sodium fluoride, glycerin, citric acid, sodium citrate, xylitol, sodium saccharin, poloxamer 407, combination mint flavor, cocamidopropyl betaine, raul amine oxide, CuSO ₄ .5H ₂ O, , Ginseng extract, golden / ginseng extract, and purified water.
4. The composition of claim 3, wherein the betaine is selected from the group consisting of alkyl-N-betaine, alkyl-N-sulfobetaine, acyl-N-betaine, alkyl N-substituted aminopropionic acid, and alkylimidazolinium betaine. Or more of the composition.
9. The composition for enhancing oral hygiene according to claim 8, wherein the betaine has the following structural formula (1) or (2)

(Formula 1)

(2)
In the above Chemical Formulas 1 and 2, R is a radical of a higher alkyl group of 10 to 18 carbon atoms selected from the group consisting of decyl, undecyl, lauryl, tridecyl, myristyl, cetyl, stearyl, isostearyl and oleyl .
4. The method of claim 3, wherein the amine oxide is selected from the group consisting of alkyl-N, N-dimethylamine oxides of Formula 3, alkyl-N, N-dihydroxyethylamine oxides of Formula 4 and acylamide t- Wherein the composition is at least one selected from the group consisting of:

(Formula 3)

(Formula 4)

(Formula 5)
11. The method of claim 10 wherein the amine oxide is selected from the group consisting of decyl-N, N-dimethylamine oxide, lauryl-N, N-dimethylamine oxide, stearyl- N-dihydroxyethylamine oxide, cetyl-N, N-dihydroxyethylamine oxide, oleyl-N, N-dihydroxyethylamine oxide, N, N-dihydroxy Ethylamine oxide, and oleyl-N, N-dihydroxyethyl-amine oxide.