KR20160085231A - Propionibacterium Acnes Antimicrobial and Cosmetics Therewith - Google Patents

Abstract

The present invention relates to an antimicrobial agent for acne, which is parasitic in a hair follicle or sebaceous gland and which can cause skin diseases and cause acne to disappear without adverse effects such as skin irritation or rash, and cosmetics containing the same, wherein a branching powder (Solanum melongena L). ≪ / RTI >

Description

Acne antimicrobial agents and cosmetic compositions containing the same <br> <br> <br> Patents - stay tuned to the technology Antimicrobial acne antimicrobials and cosmetics containing them {Propionibacterium Acnes Antimicrobial and Cosmetics Therewith}

The present invention relates to an antimicrobial agent for acne, comprising an ethanol extract of Solanum melongena L powder, and a cosmetic containing the same. More particularly, the present invention relates to an antimicrobial agent for acne, which causes skin diseases and causes acne in a hair follicle or sebaceous glands The present invention relates to an ethanol extract of Solanum melongena L powder which can be killed without side effects such as skin irritation or rash and a cosmetic product containing this extract and capable of preventing acne and reducing pigmentation of acne spot.

As the economy develops and the level of living improves, the desire for beauty grows. In pursuit of beauty of the skin, it has become a natural phenomenon, and studies that make skin beautiful have been actively conducted in various fields. Recently, acne bacterium has been actively studied among various factors affecting the skin.

Acne is a chronic inflammatory disease of the follicular pouch that affects most young adults and adults. This pathology varies according to location and severity. Young acne is moderate or mild in 85% of cases. Among 15% of severe acne, 3% to 4% of men and 0.4% of women have nodular and chronic acne. A common element in various locations is sebum residues, which appear clinically as specific basal lesions.

This pathology may be manifested by the presence of an identity lesion, a surface inflammatory lesion (pustule and pustule), or a deep inflammatory lesion (nodule) that corresponds to the enlarged folliculocyst in various stages of progression,

Acne lesions leave permanent atrophic scars, hypertrophic scars, or, in general, transient and / or pigmented irritation spots.

Acne is a follicular disease (a very specific pouch with short hair) and is closely related and involves four sequential factors: hyperplasia, hyperkeratosis, Propionibacterium acnes (P. acnes) proliferation and inflammation do.

And boring (sebaceous glands) are the earliest factors of acne. This is hormone-specific and androgen-dependent. Indeed, sebaceous glands are promoted and maintained by dihydrotestosterone (DHT) produced in sebaceous cells by 5-alpha-reductase type 1 in free testosterone. In acne, pathogens are caused by the specific sensitivity of sebaceous glands to androgens due to circulating androgens present at normal levels and partially localized 5-alpha-reductase overactivity.

The sebum consists of a mixture of squalene (12%), wax (26%), cholesterol, cholesterol esters and triglycerides. The sebum of acne skin has a higher concentration of fatty acid and squalene oxide (inflammation-promoting) compared to individuals without acne, due to the triglyceride hydrolysis of lipase secreted by P. acnes. At the same time, linoleic acid (anti-inflammatory) is deficient in cottonseed when compared to the immediate surface of the skin.

This reversal of inflammation-promoting and anti-inflammatory lipid balance is the cause of acne, which is the cause of ovarian epithelial keratinization disorders leading to cotton formation. On the other hand, squalene peroxide activates inflammatory lipid transporters (increasing leukotriene B4 and prostaglandin E2) and IL-6 production. On the other hand, the reduction of linoleic acid leads to keratinization and makes the epidermis more permeable and more vulnerable, promoting microbial growth and the production of cytokines (especially interleukin-1 alpha) and chemotactic substances that lead to inflammation.

And keratinization are abnormal proliferation of cells (keratinocytes) along the inner wall of the follicular pouch. The keratinocytes are very rapidly dissociated to induce microfacial formation. Follicular branch occlusion occurs in the subluxation area. This is due to abnormal growth, adhesion and differentiation of keratinocytes, which are not separated from each other and block the tube. In normal conditions, the keratinocytes are separated, and the sebum passes freely through the cavity. And in the case of keratinization, keratinocytes adhere to each other, and sebum release is disturbed. Therefore, not only is sebum excess secreted, but also the emission is limited by the narrow pores with the end cap. The sebaceous flow is trapped inside the follicle pouch and cellular tissue debris forms a hard, slightly white sebaceous plug called a microcyst or a closed skin.

In the keratinocyte hyperplasia stage, keratinocytes of follicular cysts of individuals with acne have a higher proliferation index (Ki-67 label) as compared to healthy controls. This increase in proliferation index is found in the lesion area and the apparently healthy acne area. This is particularly associated with the production of IL-l [alpha], the cause of micro-facial features (hyperproliferation and aberrant differentiation).

As an adhesion molecule, integrin is an adhesion molecule that enables aggregation between keratinocytes. This specifically regulates keratinocyte proliferation and migration. In acne, there is a change in integrin alpha 2, alpha 3 and alpha 5 expression by the lower keratinocytes of the acinar cells. These changes also contribute to microfacial formation.

In the process of forming sebum, hyperphosphorylation reduces the concentration of linoleic acid in sebum by dilution. Such an increase in linoleic acid deficiency and free fatty acid abnormally induces keratinocyte lower nodule differentiation, which also contributes to the formation of microfilament and triggers inflammation.

Inflammation plays a fundamental role in acne because it causes pathological progression, especially inflammatory lesions as well as scarring.

These inflammatory processes are the result of multiple and direct or indirect P. acnes proliferation. P. acnes is a symbiotic of the epidermis. In normal skin, P. acnes develops from the base of the follicle and reaches the surface of the epidermis through sebum. In acne, abnormal accumulation of keratinocytes and excessive sebum in cystic ducts cause an abnormal environment of P. acnes proliferation and cause an inflammatory reaction during pathology. P. acnes produces a variety of chemotactic factors, inflammation-promoting molecules and proteases that cause acne inflammation.

On the other hand, lipases produced by P. acnes hydrolyze sebum triglycerides into free fatty acids, which stimulate chemotactic substances and release them towards granulocytes. These neutrophil granulocytes emit a rysosomal enzyme in the surrounding tissue of the hair follicle, which ruptures the folliculocyte wall and completely diffuses the inflammation. On the other hand, P. acnes has been shown to induce inflammatory cytokines (IL-1α, TNFα, IL-6, IL-8, TGFα) using immediate immune responses in immune cells via Toll-like receptors (TLR2 and TLR4) Release induces the abrupt activity of the cells. The keratinocytes are also directly involved in the secretion of cytokines, particularly IL-1α, TNFα and chemokine IL-8, after P. acnes stimulation. Increased production of IL-8 is associated with hyperkeratosis of the cysts and causes inflammatory cells to rush toward the follicular parenchyma (chemotactic effect). TLRs and transcription factors by P. acnes NFκB activity also induces MMP (especially MMP-1, MMP-2, MMP-3 and MMP-9) release. These proteases attack connective tissue fibers around the folliculi cage and are involved in inflammation spreading from the dermis as well as softening and rupturing of the expanded cyst wall. These also cause scar formation.

In addition, in acne patients who are susceptible to developing scarring, the inflammatory response continues to be more severe with high blood vessel production. In this respect, vascular endothelial growth factor (VEGF) increases vascular permeability to stimulate the skin, induces inflammatory / endothelial cells and promotes angiogenesis to actively promote these lesions. P. acnes acts as a superantigen that directly binds to receptors and directly activates T lymphocytes without interfering with antigen-presenting cells, thus activating the working cells more rapidly and more effectively. Thus, the inflammatory response is amplified. Ultimately, the prolonged inflammatory response is an important cause of the appearance of papules, pustules and nodules.

P. acnes is known as an acne bacterium called Demodex foliumurum and Demodex brevis. Demodex folicurum is mainly parasitic in the hair follicle, the body is relatively long, Demodex brevis is mainly parasitic in the shallow sebaceous glands, and the body is relatively short. The acne bacteria found in the sebaceous glands and sebaceous glands of the adult eyelids, nose, ear canal, and hair follicles are found to have translucent milky white color, and male and female are separately present and mean length is 0.28 mm The thickness is about 1 / 2-2 / 3 of human hair. It has a stepped head and eight coarse short legs, four on each side, and has a long tail. The shape of the acne bacterium has different characteristics, so it does not spread between human beings or between different animals, but when they come into contact with each other or between the same animals, they become infected and become parasitic continuously. The life of acne bacterium is parasitic in hair follicles and sebaceous glands without intermediate hosts, and all the growth is progressed. The male and female parts are separated and mostly mate at the skin outside the hair follicle and enter the nearby hair follicles or sebaceous glands. The female has a reproductive door in front of the abdomen, and the male has a bifurcated genital between the second and third legs. Reproductive power is very strong, so that male and female breeds 50-60 eggs after 12 hours of mating, becomes an adult in 2 weeks, and lives in the skin for 30-60 days after becoming an adult. In the hair follicles and sebaceous glands, the tissue cells are pierced with nippers to absorb the nutrients and grow and develop. All of these processes are carried out in the hair follicles and sebaceous glands, and secretion feces and debris from the acne bacteria accumulate to pollute the pores and sebaceous glands. do. Acne fungus, which hates light and loves warm, is mainly active at night when a person is asleep. The higher the age, the higher the parasitism rate of acne, and the more frequent the hair cleansing cycle, the lower the parasitism rate and the less the sex and the family history.

As a result of studies on acne bacteria, acne bacteria are parasitized on hair and skin, and not only acne but also acne, spots, freckles, spots, And enlargement of the pores.

To summarize several papers that have been studied so far, acne bacteria can cause acne, spots, freckles, skin spots, enlargement of pores, itching, acne, skin aging, atopic dermatitis and various other skin irritations .

First, when the acne bacterium moves on the sebaceous glands, enters and exits the pore, and crawls over the skin, the skin feels itchy. Acne secretions, feces, dead cells and their wastes cause physiochemical reactions that cause serious skin diseases and keratinization. Acne bacteria are parasitic on the dermis layer and eat collagen and elastin of the dermis layer and destroy the vascular cord entangled in the dermis layer and deteriorate the self-vitality of the skin.

Second, many nutrients contained in various cosmetics used for skin are used as nutrients of many acne bacteria before they are absorbed into the skin, thereby suppressing nutrition of the skin.

Third, when acne bacteria of about 1 / 2-2 / 3 of the thickness of hair are piled up in the pores of one to seven or eight, the pores are stretched out, the skin is stretched and the wrinkles are increased at the same time as the skin is slackened. .

Fourth, acne bacterium causes skin pigmentation. Pigmentation such as spots, freckles, and black spots is caused by various contaminants in the pores, cosmetic residue, and debris from the acne bacterium that have reached the end of its life spoil and rot in the pores, stimulating the skin to promote the secretion of melanin pigment, This is a phenomenon caused by interference.

Fifth, acne bacteria are parasitic on sebaceous glands and digest collagen and elastin, which are the main components of dermis layer, and destroy cellular tissues and capillaries to promote premature aging of skin.

Sixth, patch test was conducted to find the cause of allergic reaction in adults with atopic dermatitis.

Seventh, Acne bacterium spreads pores through the pores, worsens the hair follicle, causing acne.

The acne treatment method is to treat the acne with only mild acne or cottonseed, and the retinoids such as retinoids are treated with topical application method. In case of mixed mucosa with papules, papules and pustules, they are treated with topical antibiotics, It has been known that oral treatment with isotretinoin for systemic acne or severe acne without acne.

However, such treatment methods have problems in that they cause skin irritation and side effects such as malformation.

It is an object of the present invention to solve the problems of the conventional antimicrobial agent using the conventional herbal medicine extract as a main ingredient and to provide a natural product-extracting acne antimicrobial agent having a killing effect against acne bacteria without skin irritation and cosmetics containing the same. have.

Another object of the present invention is to provide a method for treating skin diseases (pemphigus, psoriasis, atopic dermatitis, etc.) by applying to not only scalp of human but also pores and sebaceous glands distributed throughout the body, ), Acne, spots, freckles, spots, enlargement of pores, and the like.

The antimicrobial agent according to the present invention for achieving the above object is characterized by containing an ethanol extract of Solanum melongena L powder.

The ethanol extract of eggplant powder may further contain ethanol extract of lupine seed powder or lupine seed pod powder.

The egg yolk ethanol extract may be further mixed with an ethanol extract of Omiza powder.

The ethanol used for the ethanol extraction is high-temperature ethanol heated to 35 ° C to 78 ° C.

To achieve the above-mentioned object, the cosmetic product according to the present invention is characterized in that the extract mixture is added to the anti-acne cosmetic at a concentration of 1-20%.

Since the antimicrobial agent of the present invention having the above composition has no irritation to the skin and side effects, there is no feeling of use, skin rash after use, and not only scalp but also follicles or sebaceous glands distributed throughout the body, It can treat pemphigus, psoriasis, atopic dermatitis and the like resulting from the killing of the acne causing the skin lesion, and it can treat acne, spots, freckles, spots and pores enlargement. When applied to the scalp, It is possible to prevent the damage of the hair follicle wall by the bacteria, to prevent the cause of the nutrient loss in the hair follicle and to prevent the growth of the pore, thereby reducing the occurrence of acne and the pigmentation of the acne area.

Hereinafter, the acne antibacterial agent and the cosmetic containing it according to the present invention will be described in detail.

The acne antimicrobial agent according to the present invention comprises an ethanol extract of egg yolk powder extracted by adding ethanol heated at 35 ° C to 78 ° to egg yolk powder.

Egg powder ethanol extract can be further mixed with an ethanol extract of lupine seed powder or lupine seed pod powder. The mixing ratio between the ethanol extract of eggplant powder and the ethanol extract of lupine seed powder or lupine seed is 1:99 to 99: 1% by weight.

The ethanol extract of eggplant powder can be further mixed with eggplant powder ethanol extract. The mixing ratio between the ethanol extract of eggplant powder and the ethanol extract of eggplant powder is 1:99 to 99: 1% by weight.

Eggplant powder ethanol extract can be further mixed with ethanol extract of lupine seed powder or lupine seed pod powder and ethanol extract of omija powder. The mixing ratio between the ethanol extract of eggplant powder and the ethanol extract of eggplant powder is 1:99 to 99: 1% by weight. In this case, the mixing ratio is 1 to 99% by weight based on the total weight of the extract. Ethanol used as an extraction solvent is preferably ethanol having a purity of 95% or more.

The branch according to the present invention (Solanum melongena L) is a one-year-old herbaceous plant, the height of the stem is 60-100 cm in height, and the elliptical leaves are staggered and purple flowers bloom in June-September. Fruits are obovate (egg shaped) or cylindrical shaped fruit (奬果: fruit with lots of flesh and water and seeds in the inside). It is usually black, but it is white and yellow.

The method for producing an acne antimicrobial agent according to the present invention comprises the steps of 1) drying and pulverizing branches to prepare a powder state, 2) adding 95% or more of ethanol as an extraction solvent in a weight ratio of 1: 3 to 1:10 And immersing it for 1 to 60 days while heating at 35 DEG C to 78 DEG C to obtain an extract solution. The method for producing an acne antimicrobial agent according to the present invention may further comprise a concentrated pulverization step of obtaining a branching extract solution, 3) filtering and concentrating the branching extract solution, and then lyophilizing the extract solution by freeze drying. Heating the ethanol to 35 ° C to 78 ° C is necessary to selectively extract lupeol from the branch.

The method for producing an acne antimicrobial agent according to the present invention comprises the steps of 1) drying and then pulverizing a pod of a lupine seed or a lupine seed to prepare a powder; 2) preparing a lupine seed powder or a lupine seed pod, Ethanol at a weight ratio of 1: 3 to 1:10 and immersing the solution in a temperature of 35 ° C to 78 ° C for 1-60 days to obtain a pod extract solution of lupine seed or lupine seed. The method for producing an acne antimicrobial agent according to the present invention may further comprise the step of obtaining a pod extract solution of lupine seed or lupine seed, 3) a concentrated pulverization step of filtering and concentrating the extract solution, followed by lyophilization through lyophilization have. The method for producing an antimicrobial agent according to the present invention comprises the steps of: 4) mixing a branched extract solution or an extract solution of the concentrated powder and a pod of a lupine seed or a lupine seed or a concentrated powder thereof at a weight ratio of 1:99 to 99: 1 Mixing step. Heating the ethanol to 35 &lt; 0 &gt; C to 78 &lt; 0 &gt; C is necessary to selectively extract lupeol from the poultice of lupine seed or lupine seed.

The method of producing an acne antimicrobial agent according to the present invention comprises the steps of 1) drying and pulverizing omiza to prepare a powder form, 2) adding 95% or more ethanol as an extraction solvent to the pod of Omiza at a weight ratio of 1: 3 to 1:10 And immersing it for 1 to 60 days while heating at 35 DEG C to 78 DEG C to obtain an extract of Omija. The method for producing an acne antimicrobial agent according to the present invention may further comprise a concentrated pulverization step of obtaining an omija extract solution, 3) filtering and concentrating the extract solution, followed by lyophilization by pulverization. Also, the method for producing an acne antimicrobial agent according to the present invention comprises 4) mixing a branch extract solution or a concentrated solution thereof with an extract solution of pomegranate or a concentrated powder thereof at a weight ratio of 1:99 to 99: 1 . Heating the ethanol to 35 ° C to 78 ° C is necessary to selectively extract Loupeol from Omija.

The method for producing an acne antimicrobial agent according to the present invention comprises mixing the branch extract solution prepared in each of the above steps, the pod extract solution of lupine seed or lupine seed and the extract of Omija in an amount of 1 wt% to 99 wt% Or 1% by weight to 99% by weight of each of the bifurcated powder prepared in each step and the poddered powder of lupine seed or lupine seed and the total mass of the ozonized powder, respectively.

The cosmetic product according to the present invention is obtained by adding the above extract mixture to an anti-acne cosmetic at a concentration of 1-20%.

Hereinafter, concrete examples of the acne antibacterial agent and cosmetic product according to the present invention will be described in detail.

&Lt; Example 1 >

a) Lupine seeds which did not separate branches and pods, 95% ethanol extracts of omija - branches, lupine seeds which did not separate pods, and 2 ㎏ of powdered omija were prepared. 8 kg of 95% ethanol was added to each branch, and the mixture was immersed at 45 ° C for 10 days, and then 6 kg of each extract solution was obtained.

b) Formation of the mixture: A part of the extract solution extracted from the above method is taken out and mixed with a pure extract solution, a 1: 1 mixed solution of branch extract solution and lupine seed extract solution, a 1: 1 mixed solution of branch extract solution and Omija extract solution, A 1: 1: 1 mixed solution of branched extract, lupine seed extract and Omija extract was obtained.

After attaching the electron microscope to the CCD camera and connecting it to the computer, the sebaceous glands were compressed by pressing the sebaceous glands from the sebaceous glands next to the nose of the subject. The sebaceous glands were placed on a slide glass and the vegetable oil was dropped to dissolve the sebaceous glands. And each of the above extracts or mixed solutions was diluted 50 times, dropped on the specimens, covered with cover glass, and observed. It has been confirmed that about 4 hours have elapsed from the time when the appearance of acne bacterium is sharply shrunk, the movement is stopped, and it is disintegrated and nothing is seen. In the above experiment, the extinction test of the acne bacterium was conducted by diluting the extract solution or the mixed solution according to the present invention 50 times to confirm whether the acne bacterium was killed at a concentration that can be used as an external agent such as a cosmetic or a quasi-drug.

c) The antimicrobial agent obtained in Example 1 or 2 was added to the anti-acne cosmetic at a concentration of 1-20% by weight to obtain a cosmetic having an anti-acne function. The acne antimicrobial agent according to the present invention is added to the formulation for cosmetic use such as cream, essence, skin, gel, pawn cleansing, pack, tonic and the like at a concentration of about 1% -20%. It was confirmed that it is preferable to mix the acne antimicrobial agent according to the present invention with cream or foam cleansing at a concentration of 5%, wash it after lapse of 20 minutes so that the sterilizing component acts on the acne bacterium without washing it immediately after applying foam on the skin. It is desirable to apply a pack or tonic type formulation that is free of oil to the acne that is primarily associated with oily skin.

The clinical test results of the acne antimicrobial agent according to the present invention were as follows.

The patients who had severe acne were randomly assigned to one of the following treatments: age, sex, age, sex, age, sex, age, sex, And there were no side effects such as rashes.

Claims (5)

An antimicrobial agent for acne characterized by containing ethanol extract of eggplant powder (Solanum melongena L).
The method according to claim 1,
An acne antimicrobial agent further comprising an ethanol extract of lupine seed powder or poultice seed powder.
The method according to claim 1,
An acne antimicrobial agent containing an acne antimicrobial agent further comprising an ethanol extract of an omia powder.
4. The method according to any one of claims 1 to 3,
Wherein the ethanol used for the ethanol extraction is hot ethanol heated to 35 占 폚 to 78 占 폚.
A cosmetic comprising any one of the antimicrobial agents according to any one of claims 1 to 3.